一种用于免疫印迹的双膜分区抗体同步亲和反应装置
By using a transparent septum and a transparent reaction chamber with a microporous structure in the immunoblotting device, the problems of insufficient simultaneous incubation and washing of two membranes were solved, achieving efficient antibody reactions and accurate experimental results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHENZHEN NANSHAN DISTRICT PEOPLES HOSPITAL
- Filing Date
- 2025-08-18
- Publication Date
- 2026-07-17
AI Technical Summary
Existing incubation devices cannot effectively solve the problems of stacking, squeezing, and friction when two membranes are incubated simultaneously, which leads to blocked antibody binding and insufficient membrane washing, affecting detection accuracy and efficiency.
A removable transparent partition is installed inside a transparent reaction tank. The partition has a microporous structure to achieve physical isolation between the two membranes and uniform distribution of the antibody reaction solution, avoiding fluid dead zones and ensuring thorough washing.
This technology enables simultaneous incubation and efficient washing of two membranes, improving detection accuracy and research efficiency while reducing the occurrence of nonspecific bands and fluorescent background.
Smart Images
Figure CN224518731U_ABST
Abstract
Claims
1. A dual membrane partitioned antibody simultaneous affinity reaction device for immunoblotting, characterized by, include: Transparent reaction tank (1); A positioning baffle (2) is installed on the inner wall of the transparent reaction tank (1); A transparent partition (3) is provided with a microporous structure; the transparent partition (3) is detachably connected to the top of the positioning baffle (2); the transparent partition (3) is in contact with the inner wall of the transparent reaction tank (1); a gap is provided between the transparent partition (3) and the inner bottom wall of the transparent reaction tank (1).
2. The dual membrane partitioned antibody simultaneous affinity reaction device for immunoblotting of claim 1, wherein: The transparent partition (3) has a tweezers gripping hole (4).
3. The dual membrane partitioned antibody simultaneous affinity reaction device for immunoblotting of claim 1, wherein: The microporous structure includes a plurality of first micropores (5) and a plurality of second micropores (6), wherein the diameter of the first micropores (5) is larger than the diameter of the second micropores (6), and the plurality of first micropores (5) and the plurality of second micropores (6) are arranged alternately.
4. The dual membrane partitioned antibody simultaneous affinity reaction device for immunoblotting of claim 3, wherein: The diameter of the first micropore (5) is 2.00 mm to 3.00 mm; the diameter of the second micropore (6) is 1.00 mm to 2.00 mm; and the thickness of the transparent partition (3) is 0.5 mm to 1.5 mm.
5. The dual membrane partitioned antibody simultaneous affinity reaction device for immunoblotting of claim 1, wherein: The transparent reaction tank (1) has a length of 120mm to 160mm and a width of 80mm to 110mm; the positioning baffle (2) has a length of 5mm to 9mm and a width of 2.0mm to 3.0mm.
6. The dual membrane partitioned antibody simultaneous affinity reaction device for immunoblotting of claim 2, wherein: The diameter of the through hole (4) that the tweezers are used to grasp is 6.00 mm to 10.00 mm.
7. The dual-membrane regional antibody simultaneous affinity reaction device for immunoblotting according to claim 1, characterized in that: The transparent reaction tank (1) is a transparent polystyrene reaction tank, the transparent partition (3) is a transparent polystyrene partition, and the positioning baffle (2) is a transparent polystyrene positioning baffle.
8. The dual membrane partitioned antibody simultaneous affinity reaction device for immunoblotting of claim 1, wherein: The transparent reaction tank (1) is detachably connected to a lid (7).