An enzyme digestion reaction thermostat
By designing regulating and auxiliary devices in the constant temperature bath for the enzymatic digestion reaction, the problem of heat loss during the enzymatic digestion reaction was solved, and stable temperature control and efficient enzymatic digestion reaction were achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- NANTONG CHANGLI BIOPHARMACEUTICAL CO LTD
- Filing Date
- 2025-08-18
- Publication Date
- 2026-07-21
AI Technical Summary
When existing enzyme digestion reaction thermostats are operated at 37°C, the open chamber continuously loses heat through air convection and thermal radiation, leading to fluctuations in enzyme activity, reduced digestion efficiency, and experimental failures.
An enzyme digestion reaction constant temperature bath was designed. The opening and closing of the bath cavity is controlled by a closing plate in the adjustment device, and the sealing gasket and positioning plate are used to fix it to reduce heat loss. The auxiliary device ensures the stable connection of the water inlet pipe and the water outlet pipe to avoid temperature fluctuations.
It significantly reduced heat loss, stabilized temperature control, improved the efficiency and success rate of enzymatic digestion reactions, and avoided the generation of nonspecific bands.
Smart Images
Figure CN224530913U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of thermostats for enzyme digestion reactions, and in particular to a thermostat for enzyme digestion reactions. Background Technology
[0002] Enzyme digestion reaction thermostat is a highly efficient, precise, and reliable device that provides a stable temperature-controlled environment for enzyme digestion reactions, thereby ensuring the smooth progress of the reaction. It is commonly used in biochemical experiments, molecular biology research, and the pharmaceutical and food industries. It maintains optimal reaction conditions by precisely controlling the temperature to ensure that the enzyme digestion reaction can be carried out in an ideal environment, and it is quite common in daily life.
[0003] Existing technologies, such as the utility model patent with publication number CN211725834U, disclose a semiconductor temperature-controlled thermostatic bath. This patent includes a housing, an integrated head, a thermostatic pool, and a thermostatic bath body. The housing houses a motor and the thermostatic bath body, and the thermostatic bath body contains an oil tank. A fixing block is provided around the outer wall of the oil tank, and the oil tank is fixedly connected to a semiconductor temperature control device via the fixing block. This utility model uses a semiconductor chip for temperature control, resulting in a simple product structure and convenient maintenance. It allows the thermostatic bath to quickly reach a predetermined temperature with a maximum temperature difference of up to 400 degrees Celsius, enabling the thermostatic bath to adapt to chemical reactions within a multi-temperature range. By electrically connecting the semiconductor chip to the motor, and with a commutator inside the motor that can change the direction of the DC power supply through the semiconductor chip, the cold and hot ends of the semiconductor chip can be switched. This allows for both cooling and heating of the liquid medium inside the oil tank by using the semiconductor chip.
[0004] In the constant temperature bath equipment for enzyme digestion reaction, it was found that since the enzyme digestion reaction is usually carried out at 37°C, the open chamber will continuously lose heat through air convection and thermal radiation during the process, which will lead to fluctuations in enzyme activity, reduced digestion efficiency, and the generation of non-specific bands or even experimental failure. Utility Model Content
[0005] The purpose of this invention is to address the shortcomings of existing technologies, such as enzyme digestion reactions typically being performed at 37°C, during which the open chamber continuously loses heat through air convection and thermal radiation, leading to fluctuations in enzyme activity, reduced digestion efficiency, the generation of nonspecific bands, and even experimental failure.
[0006] To solve the above-mentioned technical problems, this utility model provides an enzyme digestion reaction constant temperature bath, comprising: a body, a cavity provided inside the upper end of the body, a stirring rack provided inside the cavity, a control processor mounted on the upper surface of the body, a water inlet pipe provided on the upper surface of the body corresponding to the cavity, the bottom end of the water inlet pipe being fixedly connected to the interior of the cavity, a water outlet pipe provided on one side of the upper surface of the body, the water outlet pipe and the water inlet pipe being fixedly connected by the same connecting pipe, the connecting pipe being a plastic flexible hose, a water tap installed on one side of the surface of the body, and an adjustment device provided on the surface of the body corresponding to the cavity, the adjustment device including a fixed frame, the bottom surface of the fixed frame being fixedly connected to the upper surface of the body, a rotating frame being rotatably connected to the inner wall of the fixed frame, one end of the rotating frame being fixed. A closing plate is connected, and a connecting block is fixedly connected to one end surface of the closing plate. The two sides of the connecting block are concave. The position of the closing plate corresponds to the position of the slot cavity. The lower surface of the closing plate abuts against the surface of the machine body. Positioning plates are fixedly connected to both sides of the end of the closing plate away from the fixing frame. The cross-section of the positioning plate is "U". A connecting plate is fixedly connected to the surface of the machine body at the position corresponding to the positioning plate. A moving rod slides through the surface of the connecting plate. A retaining frame is fixedly connected to the end of the moving rod near the positioning plate. The cross-section of the retaining frame is "U". A positioning post is fixedly connected to the inner wall of the end of the retaining frame near the positioning plate. The arc surface of the positioning post is inserted into the surface of one end of the positioning plate. A spring is sleeved on the arc surface of the moving rod. The two ends of the spring are fixedly connected to the moving rod and the connecting plate, respectively.
[0007] The effect achieved by the above components is as follows: during the operation of the enzyme digestion reaction constant temperature bath equipment, the open chamber will continuously lose heat through air convection and thermal radiation. At this time, the chamber can be opened and closed by means of the closing plate in the regulating device, so that the closing plate forms a physical barrier, significantly reducing heat loss and reducing the burden on the temperature control system.
[0008] Preferably, both ends of the inner wall of the fixed frame are fitted with coil springs, and the two ends of the coil springs are fixedly connected to the fixed frame and the rotating frame, respectively.
[0009] The effect achieved by the above components is that the torsional force generated by the coil spring can squeeze and limit the position of the rotating frame, which facilitates the position limit protection of the entire closed plate.
[0010] Preferably, a sealing gasket is fixedly connected to one side surface of the closed plate corresponding to the groove cavity. The sealing gasket has a rectangular frame cross-section and is a rubber gasket.
[0011] The effect achieved by the above-mentioned components is that when opening and closing the closing plate and the cavity for protection, they can be used in conjunction with the closing plate using a rubber sealing gasket.
[0012] Preferably, one end surface of the two movable rods is fixedly connected to the same auxiliary rod, and the cross-section of the auxiliary rod is U-shaped.
[0013] The effect achieved by the above-mentioned components is that when the position of the moving rod is pulled and moved, it can be operated with the help of the auxiliary rod fixed on the surface of the moving rod.
[0014] Preferably, auxiliary devices are provided at the corresponding positions of the inlet and outlet pipes on their arc surfaces. Each auxiliary device includes a mounting bracket, the inner wall of which is fixedly connected to the arc surface of the inlet pipe. A rotating shaft is rotatably connected to the surface of the mounting bracket. The arc surfaces at both ends of the rotating shaft are provided with threads in opposite directions. Adjusting plates are threaded through the arc surfaces at both ends of the rotating shaft. A pressing plate is fixedly connected to the upper surface of the adjusting plate. The pressing plate has an arc-shaped cross-section. A connecting shaft is fixedly connected to one end of the rotating shaft. The arc surface of the connecting shaft is provided with anti-slip texture.
[0015] The effect achieved by the above components is as follows: during the water circulation process between the inlet and outlet pipes, the auxiliary device will be used to position and protect both ends of the connecting pipe to prevent the connecting pipe from becoming loose or falling off. At this time, the extrusion plates set at both ends of the rotating shaft will be used to extrude and limit the arc surface of the connecting pipe.
[0016] Preferably, a plurality of protrusions are fixedly connected to the side of the extrusion plate near the surface of the connecting pipe. The cross-section of the protrusions is arc-shaped, and the surface of the protrusions abuts against the surface of the connecting pipe.
[0017] The effect achieved by the above-mentioned components is that the arc-shaped protrusions can effectively compress and limit the surface of the connecting pipe.
[0018] Preferably, a connecting plate is fixedly connected to one side of the bottom end of the adjusting plate, and a limiting rod is fixedly connected to the surface of the mounting bracket, with the arc surface of the limiting rod slidingly penetrating the surface of the connecting plate.
[0019] The effect achieved by the above-mentioned components is that when the position of the extrusion plate is moved and adjusted, the limiting rod can be used for auxiliary guidance and protection.
[0020] Compared with related technologies, the enzymatic digestion reaction constant temperature bath provided by this utility model has the following beneficial effects:
[0021] By operating the adjustment device, the surface of the tank cavity can be opened and closed. By flipping and fixing the position of the closing plate, the positioning plates fixed on both sides of one end of the closing plate are inserted and fixed with the sliding adjustment frame and the locking post on the upper surface of the machine body. This allows the entire closing plate to be positioned, which helps to avoid interference from the outside air, reduce temperature fluctuations, and facilitates precise control of the reaction temperature.
[0022] By operating the auxiliary device, water circulation between the inlet and outlet pipes is achieved, ensuring the stability of the tank temperature. At this time, the squeezing plates on both sides of the pipe can effectively limit the two ends of the entire connecting pipe, preventing the connecting pipe from loosening and falling off, thus preventing liquid leakage. Attached Figure Description
[0023] Figure 1 A schematic diagram of the structure of an enzyme digestion reaction constant temperature bath provided by this utility model;
[0024] Figure 2 for Figure 1 A partial structural diagram of the three-dimensional structure shown;
[0025] Figure 3 for Figure 1 The diagram shows the structure of the regulating device.
[0026] Figure 4 for Figure 1 The enlarged structural diagram at point A is shown below;
[0027] Figure 5 for Figure 1 The enlarged structural diagram at point B is shown.
[0028] The following are the labeling elements in the diagram: 1. Main body; 2. Control processor; 3. Water tap; 4. Adjustment device; 401. Closing plate; 402. Fixing frame; 403. Coil spring; 404. Rotating frame; 405. Connecting block; 406. Positioning plate; 407. Connecting plate; 408. Clip frame; 409. Positioning column; 410. Moving rod; 411. Spring; 412. Auxiliary rod; 413. Sealing gasket; 5. Auxiliary device; 51. Mounting frame; 52. Rotating shaft; 53. Adjustment plate; 54. Extrusion plate; 55. Protrusion; 56. Limiting rod; 57. Connecting shaft; 58. Connecting plate; 6. Tank cavity; 7. Stirring frame; 8. Water inlet pipe; 9. Water outlet pipe; 10. Connecting pipe. Detailed Implementation
[0029] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.
[0030] The specific implementation of this utility model will be described in detail below with reference to specific embodiments.
[0031] Please see Figures 1 to 5 This utility model provides an enzyme digestion reaction constant temperature bath, comprising: a body 1, a cavity 6 provided inside the upper end of the body 1, a stirring rack 7 provided inside the cavity 6, a control processor 2 installed on the upper surface of the body 1, a water inlet pipe 8 provided on the upper surface of the body 1 corresponding to the position of the cavity 6, the bottom end of the water inlet pipe 8 being fixedly connected to the interior of the cavity 6, a water outlet pipe 9 provided on one side of the upper surface of the body 1, the water outlet pipe 9 and the surface of the water inlet pipe 8 being fixedly connected by the same connecting pipe 10, the connecting pipe 10 being a plastic hose, a water tap 3 installed on one side of the surface of the body 1, an adjustment device 4 provided on the surface of the body 1 corresponding to the position of the cavity 6, and auxiliary devices 5 provided on the arc surfaces of the water inlet pipe 8 and the water outlet pipe 9 corresponding to the positions of the connecting pipe 10.
[0032] In the embodiments of this utility model, please refer to Figure 2 , Figure 3 and Figure 4 The adjusting device 4 includes a fixed frame 402, the bottom surface of which is fixedly connected to the upper surface of the body 1. A rotating frame 404 is rotatably connected to the inner wall of the fixed frame 402. A closing plate 401 is fixedly connected to one end of the rotating frame 404. A connecting block 405 is fixedly connected to one end of the closing plate 401. The two sides of the connecting block 405 are concave. The position of the closing plate 401 corresponds to the position of the cavity 6. The lower surface of the closing plate 401 abuts against the surface of the body 1. Positioning plates 406 are fixedly connected to both sides of the end of the closing plate 401 away from the fixed frame 402. The cross-section of the positioning plate 406 is U-shaped. A connecting plate 407 is fixedly connected to the surface of the body 1 at the position corresponding to the positioning plate 406. A moving rod 410 slides through the surface of the connecting plate 407. The end of the moving rod 410 near the positioning plate 406... A frame 408 is fixedly connected, and the cross-section of the frame 408 is U-shaped. A positioning post 409 is fixedly connected to the inner wall of the end of the frame 408 near the positioning plate 406. The arc surface of the positioning post 409 is inserted into the surface of one end of the positioning plate 406. A spring 411 is sleeved on the arc surface of the moving rod 410. The two ends of the spring 411 are fixedly connected to the moving rod 410 and the connecting plate 407 respectively. Both ends of the inner wall of the fixed frame 402 are sleeved with coil springs 403. The two ends of the coil springs 403 are fixedly connected to the fixed frame 402 and the rotating frame 404 respectively. A sealing gasket 413 is fixedly connected to one side surface of the closing plate 401 corresponding to the cavity 6. The sealing gasket 413 has a rectangular frame cross-section and is a rubber gasket. The same auxiliary rod 412 is fixedly connected to one end surface of the two moving rods 410. The cross-section of the auxiliary rod 412 is U-shaped.
[0033] In the embodiments of this utility model, please refer to Figure 5 The auxiliary device 5 includes a mounting frame 51. The inner wall of the mounting frame 51 is fixedly connected to the arc surface of the water inlet pipe 8. A rotating shaft 52 is rotatably connected to the surface of the mounting frame 51. The arc surfaces at both ends of the rotating shaft 52 are provided with threads in opposite directions. An adjusting plate 53 is threaded through the arc surfaces at both ends of the rotating shaft 52. A pressing plate 54 is fixedly connected to the upper surface of the adjusting plate 53. The cross section of the pressing plate 54 is arc-shaped. A connecting shaft 57 is fixedly connected to one end of the rotating shaft 52. The arc surface of the connecting shaft 57 is provided with anti-slip texture. Several protrusions 55 are fixedly connected to the side of the pressing plate 54 near the surface of the connecting pipe 10. The cross section of the protrusions 55 is arc-shaped. The surface of the protrusions 55 abuts against the surface of the connecting pipe 10. A connecting plate 58 is fixedly connected to the bottom side of the adjusting plate 53. A limiting rod 56 is fixedly connected to the surface of the mounting frame 51. The arc surface of the limiting rod 56 slides through the surface of the connecting plate 58.
[0034] The working principle of the enzyme digestion reaction constant temperature tank provided by this utility model is as follows: A liquid medium is usually used as the heat exchange medium in the reaction constant temperature tank. The inlet pipe 8 introduces cooling water or temperature-controlled liquid into the tank cavity 6. The liquid in the tank changes temperature through heating or cooling processes and is then discharged through the outlet pipe 9. The flow and circulation of the liquid help conduct heat, thereby keeping the temperature of the reactants in the tank stable. During this process, the regulating device 4 opens and closes the entire tank cavity 6 to further avoid interference from external air and reduce temperature fluctuations. First, the rotating frame 404 inside the fixed frame 402 is flipped, allowing the rotating frame 404 to overcome the torsional force generated by the coil spring 403, thus causing the rotating frame 404 to drive the entire closing plate 401 to flip until the closing plate 401 is in contact with the surface of the tank cavity 6. At the same time, the sealing gasket 413 fixed to the lower surface of the closing plate 401 is sealed. Then, the auxiliary rod 412 is pulled, causing the auxiliary rod 412 to drive the moving rod 410 on the surface of the connecting plate 407 to overcome the tensile force generated by the spring 411, allowing the moving rod 410 to drive the clamping frame. 408 moves towards the positioning plate 406. When the positioning plate 406 flips with the help of the closing plate 401, it aligns with the frame 408. Simultaneously, the moving rod 410 is released, allowing the moving rod 410 to use the tension of the spring 411 to insert and fix the locking post on one side of the frame 408 into the inner wall surface of the positioning plate 406. At this point, the entire closing plate 401 is effectively fixed and closed. Water then circulates through the inlet pipe 8 and the outlet pipe 9. Temperature control is achieved through the inlet pipe... The auxiliary device 5 on the surface of the water inlet pipe 8 and the water outlet pipe 9 are operated. After the plastic hose connecting pipe 10 is connected to the water inlet pipe 8 and the water outlet pipe 9, the connecting shaft 57 is rotated, which drives the rotating shaft 52 to rotate. At this time, the adjusting plates 53 at both ends of the rotating shaft 52 will drive the squeezing plate 54 to squeeze and limit the surface of the connecting pipe 10, thereby effectively fixing the entire connecting pipe 10. The squeezing plate 54 is guided by the limiting rod 56 fixed on the surface of the mounting bracket 51, which can prevent the squeezing plate 54 from shifting its position.
[0035] The circuits and controls involved in this utility model are all existing technologies and will not be described in detail here.
[0036] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.
Claims
1. A thermostat for an enzyme digestion reaction, characterized in that, include: The machine body (1) has a cavity (6) inside its upper end, and a stirring rack (7) is installed inside the cavity (6). A control processor (2) is installed on the upper surface of the machine body (1). A water inlet pipe (8) is installed on the upper surface of the machine body (1) at the position corresponding to the cavity (6). The bottom end of the water inlet pipe (8) is fixedly connected to the inside of the cavity (6). A water outlet pipe (9) is installed on one side of the upper surface of the machine body (1). The surface of the water outlet pipe (9) and the surface of the water inlet pipe (8) are fixedly connected by the same connecting pipe (10). The hose is made of plastic. A water tap (3) is installed on one side of the surface of the body (1). An adjustment device (4) is provided on the surface of the body (1) corresponding to the position of the cavity (6). The adjustment device (4) includes a fixing frame (402). The bottom surface of the fixing frame (402) is fixedly connected to the upper surface of the body (1). A rotating frame (404) is rotatably connected to the inner wall of the fixing frame (402). A closing plate (401) is fixedly connected to one end of the rotating frame (404). A connecting block (405) is fixedly connected to one end of the closing plate (401). The connecting block (405) has concave cross-sections on both sides. The position of the closing plate (401) corresponds to the position of the cavity (6). The lower surface of the closing plate (401) abuts against the surface of the body (1). Positioning plates (406) are fixedly connected to both sides of the end of the closing plate (401) away from the fixing frame (402). The cross-section of the positioning plate (406) is "U". A connecting plate (407) is fixedly connected to the surface of the body (1) at the position corresponding to the positioning plate (406). A moving rod slides through the surface of the connecting plate (407). (410) A frame (408) is fixedly connected to one end of the moving rod (410) near the positioning plate (406). The cross-section of the frame (408) is "U". A positioning post (409) is fixedly connected to the inner wall of one end of the frame (408) near the positioning plate (406). The arc surface of the positioning post (409) is inserted into one end surface of the positioning plate (406). A spring (411) is sleeved on the arc surface of the moving rod (410). The two ends of the spring (411) are fixedly connected to the moving rod (410) and the connecting plate (407) respectively.
2. The enzymatic digestion reaction constant temperature bath according to claim 1, characterized in that, Both ends of the inner wall of the fixed frame (402) are fitted with coil springs (403), and the two ends of the coil springs (403) are fixedly connected to the fixed frame (402) and the rotating frame (404) respectively.
3. The constant temperature bath for enzymatic digestion reaction according to claim 1, characterized in that, A sealing gasket (413) is fixedly connected to one side surface of the closed plate (401) corresponding to the groove (6). The sealing gasket (413) has a rectangular frame cross section and is a rubber gasket.
4. The constant temperature bath for enzymatic digestion reaction according to claim 1, characterized in that, The two movable rods (410) are fixedly connected to the same auxiliary rod (412) at one end surface, and the cross section of the auxiliary rod (412) is "U" shaped.
5. The constant temperature bath for enzymatic digestion reaction according to claim 1, characterized in that, Auxiliary devices (5) are provided at the positions of the inlet pipe (8) and outlet pipe (9) corresponding to the connecting pipe (10). The auxiliary device (5) includes a mounting bracket (51). The inner wall of the mounting bracket (51) is fixedly connected to the arc surface of the inlet pipe (8). A rotating shaft (52) is rotatably connected to the surface of the mounting bracket (51). The arc surfaces at both ends of the rotating shaft (52) are provided with threads in opposite directions. An adjusting plate (53) is threaded through the arc surfaces at both ends of the rotating shaft (52). A pressing plate (54) is fixedly connected to the upper surface of the adjusting plate (53). The cross section of the pressing plate (54) is arc-shaped. A connecting shaft (57) is fixedly connected to one end of the rotating shaft (52). The arc surface of the connecting shaft (57) is provided with anti-slip texture.
6. The constant temperature bath for enzymatic digestion reaction according to claim 5, characterized in that, The extrusion plate (54) has several protrusions (55) fixedly connected to one side of the surface of the connecting pipe (10). The cross-section of the protrusions (55) is arc-shaped, and the surface of the protrusions (55) abuts against the surface of the connecting pipe (10).
7. The enzymatic digestion reaction constant temperature bath according to claim 5, characterized in that, A connecting plate (58) is fixedly connected to one side of the bottom end of the adjusting plate (53), and a limiting rod (56) is fixedly connected to the surface of the mounting bracket (51). The arc surface of the limiting rod (56) slides through the surface of the connecting plate (58).