Sample cartridge

Abstract

The application discloses a sample box for containing a frozen transmission electron microscope sample and a carrier net, and the sample box comprises a box body and a cover plate, one end of the box body is provided with an open port, at least one sample groove is formed in the box body, and a limiting portion is further arranged in the box body and arranged adjacent to the sample groove and the open port; the cover plate is arranged on the open port, at least part of the cover plate extends into the box body and abuts against the limiting portion to be spaced apart from the sample groove. Thus, the cover plate can be limited in the vertical direction and the horizontal direction, on one hand, the probability of physical damage and chemical damage of the carrier net and the sample can be reduced, the accuracy of characterization can be improved, and the experimental precision and the experimental success rate can be improved, on the other hand, the cover plate can be transferred synchronously with the box body, the opening and closing difficulty of the cover plate is lower, the use convenience of the sample box can be improved, and the sample can be quickly taken and transferred.

Description

Technical Field

[0001] This application relates to the field of sample box technology, and in particular to a sample box. Background Technology

[0002] In related technologies, sample boxes are used to hold test samples and mesh carriers. The size of the sample slot is slightly larger than the size of the mesh carrier. After the mesh carrier and sample are placed in the sample box, a cover plate needs to be added to reduce the probability of sample contamination. However, the cover plate makes the mesh carrier prone to deformation, and the cover plate is fixed to the box body, which reduces the convenience of taking out and putting in the mesh carrier. Utility Model Content

[0003] This application aims to at least solve one of the technical problems existing in the prior art. To this end, one object of this application is to provide a sample box with a more stable lid, more convenient placement and removal of the mesh, which can improve experimental accuracy and reduce the probability of mesh deformation.

[0004] This application provides a sample box for containing samples for cryo-transmission electron microscopy and a grid. The sample box includes a box body and a cover plate. One end of the box body has an open opening. At least one sample slot is formed inside the box body. The box body also has a limiting part, which is disposed adjacent to the open opening relative to the sample slot. The cover plate is disposed on the open opening, and at least a portion of the cover plate extends into the box body and is pushed against the limiting part to be spaced apart from the sample slot.

[0005] According to the sample box of the present application embodiment, by setting a limiting part and allowing at least a portion of the cover plate to extend into the box body and push against the limiting part, the cover plate can be limited in the vertical and horizontal directions. On the one hand, it can reduce the probability of physical and chemical damage to the carrier and sample, thereby improving the accuracy of characterization, and thus improving experimental precision and success rate. On the other hand, the cover plate can be transferred synchronously with the box body, and the opening and closing of the cover plate is easier, which can improve the convenience of using the sample box and facilitate the rapid retrieval and transfer of samples.

[0006] According to some embodiments of this application, the box body has a first surface, which is spaced apart from the bottom wall of the box body, and a sample groove extending toward the bottom wall is formed on the first surface. A limiting part is formed on the first surface and / or the inner side wall of the box body.

[0007] In the above technical solution, by setting a limiting part located in the accommodating space of the box and above the sample slot, the cover plate is vertically limited. The limiting part is used to connect and fix at least one of the first surface or the side wall of the box. The position of the limiting part is more reasonable and the structural stability of the limiting part is higher. This can improve the limiting effect of the limiting part on the box, thereby further reducing the probability of the cover plate causing deformation of the carrier mesh and improving the reliability of the sample box.

[0008] According to some embodiments of this application, the limiting part is constructed as a limiting boss, and the limiting boss is at least connected to the inner sidewall of the box, or the limiting part is constructed as a limiting post, and at least one end of the limiting post is connected to the first surface.

[0009] In the above technical solution, by constructing a limiting part as a limiting boss or a limiting post, the limiting part can limit the cover plate. The force distribution of the limiting boss is more uniform, which can improve the limiting and load-bearing reliability and stability of the cover plate. In the embodiment where the limiting post is used for limiting, the limiting post is easier to process and uses less material than the limiting boss, which can reduce the processing difficulty of the box and reduce material costs.

[0010] According to some embodiments of this application, the end of the limiting portion away from the first surface defines a second surface spaced apart from the first surface, and a receiving cavity is formed between the first surface and the second surface. The receiving cavity is used to receive the freezing medium so that the sample and the carrier are both immersed below the surface of the freezing medium.

[0011] In the above technical solution, by setting up the accommodating cavity, the space volume inside the box for accommodating the freezing medium is larger, which can store more freezing medium, extend the freezing time of the sample, and improve the preservation effect of the sample. Moreover, the freezing medium can completely wet the sample and the grid, and can also achieve the isolation effect of isolating the sample and the grid from the air, which can further reduce the probability of chemical damage to the sample, such as chemical reaction and deterioration, thereby further improving the preservation effect of the sample, improving the accuracy of characterization, and improving the success rate and experimental precision of subsequent experiments.

[0012] According to some embodiments of this application, there are multiple sample cells, and each sample cell is constructed as a rhomboid cell.

[0013] The above technical solution can reduce the difficulty of picking up and placing the carrier net, improve the convenience of picking up and placing the carrier net and the sample, and at the same time reduce the manufacturing difficulty of the sample tank and facilitate the demolding of the box.

[0014] According to some embodiments of this application, at least two opposing sidewall surfaces of the sample cell are constructed as a first slope.

[0015] In the above technical solution, by setting a first inclined plane, the upper opening of the sample cell can be larger than the lower opening, thereby guiding and fixing the carrier mesh. Under the guidance of the first inclined plane, the carrier mesh can gradually move until it is fixed in the sample cell, which can reduce the probability of the carrier mesh tipping over. This not only reduces the difficulty of picking up the carrier mesh, but also reduces the probability of puncturing the carrier mesh coating and causing damage to the carrier mesh during the picking process, thus having a lower impact on subsequent sample loading and higher characterization accuracy.

[0016] Furthermore, in the embodiment where all four sidewalls are constructed as first inclined surfaces, while achieving the same technical effect as two first inclined surfaces, the carrier mesh only contacts the sample groove through two points in its own tangential direction. The contact area is smaller, the probability of stress deformation of the carrier mesh is lower, the probability of physical damage to the carrier mesh is lower, the impact on subsequent sample loading is smaller, and the characterization accuracy can be further improved.

[0017] According to some embodiments of this application, the angle formed by the intersection of the two edges on the long diagonal of the sample groove is the first edge angle, which is 20°-30°, and the groove depth of the sample groove is 3.4mm-4.3mm.

[0018] Specifically, the edge formed by the intersection of two adjacent first inclined surfaces on the long diagonal of the sample groove and the vertical surface perpendicular to the first surface and coinciding with the short diagonal of the sample groove form a first included angle, which ranges from 10° to 15°.

[0019] In the above technical solution, when the angle of the first included angle is greater than the upper limit of 15°, the guiding effect of the first inclined surface will be reduced. When the angle of the first included angle is less than the lower limit of 10°, the groove depth needs to be set deeper, which will not only increase the overall size of the box, but also increase the distance between the bottom of the sample groove and the first surface, making it more difficult to pick up the net in the sample groove. The more reasonable first included angle in this application can take into account both the guiding effect on the net and the convenience of picking up the net.

[0020] This application makes the angle range of the first included angle more reasonable. Under the premise that the length of the long diagonal is fixed, the short diagonal can be set to be shorter, so that more sample slots can be arranged in the first surface. At the same time, the depth of the sample slots can be made more reasonable, which can avoid the screen protruding from the first surface due to the slot depth being too shallow, thereby reducing the probability of the cover plate pressing against the screen. It can also avoid the slot depth being too deep, which would result in the screen being too far below the first surface, thus taking into account the convenience of removing the screen.

[0021] According to some embodiments of this application, a connecting groove is provided between adjacent sample cells to allow multiple sample cells to communicate with each other.

[0022] In the above technical solution, the liquid level of the freezing medium in multiple sample cells is more consistent, which can reduce the occurrence of situations where a sample cell is not completely filled with freezing medium, or even where a sample cell has no freezing medium at all. This improves the wetting and isolation effects of the sample and the carrier mesh in the sample cell, reduces air exposure and subsequent chemical damage to the sample caused by insufficient freezing medium, and improves the accuracy of characterization, especially for highly water- and oxygen-sensitive samples.

[0023] According to some embodiments of this application, the connecting slot is used to connect the short diagonal corner regions of adjacent sample slots.

[0024] In the above technical solution, the sample cell is constructed as a rhomboid cell, with one short diagonal and one long diagonal. The two edges connected by the short diagonal are called short diagonal edges, and the two edges connected by the long diagonal are called long diagonal edges. The two long diagonal edge areas are used to hold and limit the carrier and the sample. A connecting groove is further provided on the short diagonal edge area. While improving the consistency of the liquid level in multiple sample cells, the opening space on the first surface can be further widened. This makes it easier to pick up and put down the carrier and the sample through the short diagonal angle area with tweezers. The tweezers can have more room to move, which can reduce the difficulty of picking up and putting down the carrier and improve the convenience of picking up and putting down.

[0025] According to some embodiments of this application, the first center line of the box body passes sequentially through the short diagonals of a plurality of sample slots; or the short diagonals of adjacent sample slots are arranged parallel to each other in the first surface.

[0026] In the above technical solution, multiple sample slots can be connected sequentially, and the first center line of the box body can pass through the short diagonals of multiple sample slots sequentially. Alternatively, the multiple sample slots can be disconnected, and the first center line of the box body can pass through the short diagonals of multiple sample slots sequentially. This can make the load distribution within the box body more uniform, reduce the probability of the sample box tipping over, and improve the reliability and stability of sample and net transfer, as well as the preservation integrity of the net and sample. In embodiments where the short diagonals of adjacent sample slots are arranged parallel in the first plane, the sample slots can be rotated at a certain angle relative to the first center line. During the process of picking up the net, there is no need to rotate the wrist to align with the net, making the picking process more convenient and reducing the probability of the net being punctured. This not only reduces the difficulty of picking up the net but also reduces the probability of physical damage to the net or sample during the picking process, thereby improving the transfer speed and success rate.

[0027] According to some embodiments of this application, at least one sidewall of the connecting groove is constructed as a second inclined surface.

[0028] In the above technical solution, the connecting groove is constructed as a V-shaped groove, and at least one of the two side walls of the connecting groove is constructed as a V-shape, so that the top opening size of the connecting groove is larger. The connecting groove with a larger opening size is used to avoid the tweezers, which can give the tweezers more operating space and further reduce the difficulty of picking up and placing the net.

[0029] According to some embodiments of this application, a plurality of sample slots are spaced apart on the first surface.

[0030] In the above technical solution, the freezing media inside the multiple sample cells will not mix, which can reduce the probability of cross-contamination between multiple samples and improve the accuracy of characterization.

[0031] According to some embodiments of this application, two first anti-rotation limiting recesses are provided on the outer periphery of the box body, the two first anti-rotation limiting recesses are arranged opposite to each other, and a second anti-rotation limiting recess corresponding to the first anti-rotation limiting recesses is provided on the cover plate.

[0032] In the above technical solution, a pair of first anti-rotation limiting recesses are provided on the outer periphery of the box body, and a pair of second anti-rotation limiting recesses are provided on the outer periphery of the corresponding cover plate. The two symmetrical first anti-rotation limiting recesses can reduce the local outer diameter of the box body, making it easier for the tweezers to grip the outer peripheral wall of the box body, thus making it easier to handle the sample box and further reducing the difficulty of transporting the sample box. Both first anti-rotation limiting recesses can be used to cooperate with the positioning protrusions on the freezing worktable, making the positioning and cooperation between the box body and the freezing worktable easier and more convenient to operate. Furthermore, the first anti-rotation limiting recesses can cooperate with the second anti-rotation limiting recesses to limit the rotation of the cover plate, which can reduce the movement of the cover plate relative to the box body, thereby improving the stability and reliability of the box body fixing the cover plate, reducing the probability of the cover plate falling off, reducing the probability of sample contamination, and improving the accuracy of subsequent characterization.

[0033] According to some embodiments of this application, the second centerline of the box body passes through two first anti-rotation limiting recesses in sequence, and the second angle formed by the long diagonal line of the sample groove and the second centerline is 15°-30°.

[0034] In the above technical solution, the sample cell can be rotated at a certain angle relative to the second center line, which can reduce the difficulty of picking up the carrier. During the process of picking up the carrier, there is no need to rotate the wrist, reducing the degree of hand twisting and thus reducing the probability of carrier damage.

[0035] Understandably, the lower limit of the second included angle is 15°, and angles less than 15° are unlikely to effectively reduce the difficulty of handling the sample carrier. The upper limit of the second included angle is 30°, and angles greater than 30° will increase the area occupied by a single sample cell on the first surface, resulting in fewer sample cells to be arranged for the same area, making it difficult to balance the number of sample cells. In addition, angles exceeding 30° are also unlikely to effectively reduce the difficulty of handling the sample carrier.

[0036] According to some embodiments of this application, the height of the box is H, the outer diameter of the box is D, and the height-to-diameter ratio is less than or equal to 1.

[0037] In the above technical solution, making the height-to-diameter ratio of the box less than or equal to 1 can reduce the probability of the box tipping over, thereby improving the reliability and stability of the sample box in preserving the sample.

[0038] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0039] The above and / or additional aspects and advantages of this application will become apparent and readily understood from the description of the embodiments taken in conjunction with the following drawings, in which:

[0040] Figure 1 This is a schematic diagram of a sample box according to the first embodiment of this application;

[0041] Figure 2 This is a top view of the box body according to the first embodiment of this application;

[0042] Figure 3 yes Figure 2 Schematic cross-section of midline AA;

[0043] Figure 4 yes Figure 2 Schematic sectional view of the midline BB;

[0044] Figure 5 This is a top view of the box body according to the second embodiment of this application;

[0045] Figure 6 This is a schematic diagram of the cover plate according to the second embodiment of this application;

[0046] Figure 7 This is a top view of the box body according to the third embodiment of this application;

[0047] Figure 8 This is a schematic diagram of a cover plate according to the first or third embodiment of this application.

[0048] Figure label:

[0049] Sample box 100, screen 200

[0050] Box body 10, sample slot 11, limiting part 12, opening 13, connecting groove 14, first anti-rotation limiting recess 15

[0051] Cover plate 20, second anti-rotation limiting recess 21, handle 22,

[0052] First face a, second face b, first inclined plane c, second inclined plane d

[0053] Short diagonal X, long diagonal Y, box height H, box outer diameter D, first center line M, second center line N,

[0054] First included angle α, second included angle β. Detailed Implementation

[0055] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0056] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used in the description of this application is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms "comprising" and "having," and any variations thereof, in the description, claims, and accompanying drawings of this application are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the description, claims, or accompanying drawings of this application are used to distinguish different objects, not to describe a specific order or hierarchy.

[0057] In this application, the reference to "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment that is mutually exclusive with other embodiments.

[0058] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "attachment" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0059] In this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. Additionally, in this application, the character " / " generally indicates that the preceding and following related objects have an "or" relationship.

[0060] In the embodiments of this application, the same reference numerals denote the same components, and for the sake of brevity, detailed descriptions of the same components are omitted in different embodiments. It should be understood that the thickness, length, width, and other dimensions of various components in the embodiments of this application shown in the accompanying drawings, as well as the overall thickness, length, width, and other dimensions of the integrated device, are merely illustrative and should not constitute any limitation on this application.

[0061] In the description of this application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this application.

[0062] In the description of this application, the first feature being "above" or "below" the second feature may include the first and second features being in direct contact, or the first and second features being in contact through another feature between them.

[0063] In the description of this application, the terms "above," "over," and "on top" for the first feature and the second feature include the first feature being directly above or diagonally above the second feature, or simply indicate that the first feature is at a higher horizontal level than the second feature.

[0064] In this application, "multiple" means two or more (including two).

[0065] The sample cassette of a cryo-transmission electron microscopy (cryo-TEM) is used to store and transfer different numbers of samples. The disc-shaped grid is used to support the sample and is placed in the sample slot of the sample cassette. The sample is generally deposited or adsorbed on the grid.

[0066] Currently, the cryotransfer process for samples mainly focuses on preserving the sample structure. However, many samples, especially the materials used in battery preparation and the substances generated during use, are extremely sensitive to air and water. If the samples are directly exposed to air, they will undergo chemical reactions, leading to distortion of subsequent characterization results (see Li, Y. et al. Science 358, 506–510 (2017)). Therefore, in order to ensure the accuracy and success rate of characterization experiments, sample boxes are needed to ensure that the samples do not deteriorate during the transfer process.

[0067] The sample box is covered with a cover plate. The depth of the sample groove is similar to the diameter of the carrier mesh. The carrier mesh is flush with the surface of the box. The carrier mesh can move slightly inside the sample box (such as due to static electricity). When the cover plate is installed, the cover plate can easily cause the carrier mesh to be squeezed and deformed, which will affect the accuracy and feasibility of the characterization (that is, if the carrier mesh is bent, it may be impossible to place the carrier mesh into the clamping groove of the sample rod).

[0068] In some technical solutions, the cover plate designed using its own weight (Nanosoft Quick Lid Grid BoxLid) can achieve quick opening and closing of the cover plate. However, the cover plate has no fixed structure and is easy to fall off during the transfer process. It is not suitable for synchronous transfer with the box body, and the cover plate is easy to squeeze the carrier mesh, causing the carrier mesh to deform.

[0069] In other technical solutions, the cover plate needs to be fixed to the box body by screws, etc. Although the fixation reliability and stability are high, the sample box, the carrier mesh and the sample are all in a frozen environment, and the ease of disassembling and assembling the cover plate on the box body is poor, which is not conducive to the rapid and accurate transfer of samples. For materials that are particularly sensitive to characterization, such as solid electrolyte interface materials (see Zhang, Z. et al. Science 375, 66–70 (2022)), during the sample preparation and loading process, the carrier mesh immersed in liquid nitrogen in the sample box needs to be transferred to the frozen worktable, and then the carrier mesh, which is still immersed in liquid nitrogen, needs to be quickly transferred from the frozen worktable to the sample rod. Therefore, the ease of use of the sample box and the integrity of sample preservation are very important.

[0070] Based on this, this application proposes a sample box. By setting a limiting part inside the box and allowing at least a portion of the cover plate to extend into the box and push against the limiting part, on the one hand, the cover plate can be separated from the sample slot to reduce the probability of the cover plate squeezing the mesh, thereby reducing the probability of the mesh plate deforming and improving the accuracy and success rate of the experiment. On the other hand, the side wall of the box can limit the cover plate, allowing the cover plate to be directly placed on the box. The cover plate and the box can be transported synchronously, and the probability of the cover plate falling off is even lower. This not only reduces the difficulty of sample transfer and improves the preservation of the sample, but also makes the cover plate easy to install and remove, reducing the difficulty of retrieving the mesh plate and sample from the sample box.

[0071] The following is for reference. Figures 1-8 The sample box 100 according to an embodiment of this application is described.

[0072] like Figure 1-5 as well as Figure 7As shown, this application provides a sample box 100 for accommodating samples for cryo-transmission electron microscopy and a grid 200. The sample can be fixed on the grid 200 by deposition or adsorption, and the grid 200 and the sample can be synchronously set in the sample groove 11.

[0073] The sample box 100 includes a box body 10 and a cover plate 20. One end of the box body 10 has an opening 13. At least one sample slot 11 is provided inside the box body 10. The box body 10 also has a limiting part 12, which is disposed adjacent to the opening 13 relative to the sample slot 11. The cover plate 20 is placed over the opening 13, and at least a portion of the cover plate 20 extends into the box body 10 and pushes against the limiting part 12 to be spaced apart from the sample slot 11.

[0074] Specifically, at least one sample slot 11 is formed inside the box body 10, and the mesh 200 and the sample are placed in the sample slot 11. The top of the box body 10 is provided with an opening 13, and the cover plate 20 can be placed on the opening 13 to separate the internal space of the box body 10 from the outside world, reduce the probability of the sample coming into direct contact with the air, and reduce the probability of the sample being chemically damaged.

[0075] Furthermore, a limiting part 12 is provided inside the box body 10. The limiting part 12 is located above the sample groove 11 and is positioned relative to the sample groove 11 and adjacent to the open opening 13, so that the end of the limiting part 12 away from the sample groove 11 is spaced apart from the sample groove 11 in the height direction. When the cover plate 20 is placed over the open opening 13, at least part (partial or all) of the cover plate 20 can extend into the box body 10, and the side surface of the cover plate 20 facing the sample groove 11 can be pushed against the limiting part 12, so that the cover plate 20 and the sample groove 11 can be spaced apart.

[0076] It should be noted that the diameter of the carrier 200 is similar to the depth of the sample groove 11, and the cover plate 20 and the sample groove 11 can be spaced apart by the limiting part 12, thereby limiting the cover plate 20 in the vertical direction. This reduces the probability of the cover plate 20 pressing against or contacting the carrier 200, and the probability of mechanical damage to the carrier 200. It also reduces the probability of the carrier 200 being squeezed and deformed, and avoids the sample from peeling off from the carrier 200. In other words, by reducing the deformation and damage of the carrier 200, the carrier 200 can be placed flat on the cryogenic stage, resulting in better electron beam focusing, stable image resolution, improved characterization accuracy, and increased experimental success rate.

[0077] It is understood that the cover plate 20 extends at least partially into the box body 10. This could mean that the cover plate 20 partially extends into the box body 10, with the surface of the cover plate 20 protruding from the box body 10, or the cover plate 20 extends completely into the box body 10, with its upper surface located below or flush with the top surface of the box body 10. In this case, the inner wall of the box body 10 can limit the cover plate 20 in the horizontal direction, reducing its movement. This not only further reduces the probability of the cover plate 20 causing deformation of the carrier mesh 200, but also allows the cover plate 20 to be transported synchronously with the box body 10. During transport, the probability of the cover plate 20 falling off is lower, reducing the probability of chemical damage during sample transport. Furthermore, the cover plate 20 does not need to be fastened to the box body 10 with screws or other fasteners, making opening and closing the cover plate 20 easier and increasing the convenience of using the sample box 100, facilitating rapid sample retrieval and transfer.

[0078] According to the embodiment of this application, the sample box 100, by providing a limiting part 12 and allowing at least a portion of the cover plate 20 to extend into the box body 10 and abut against the limiting part 12, can limit the cover plate 20 in both the vertical and horizontal directions. On the one hand, this can reduce the probability of physical and chemical damage to the carrier net 200 and the sample, thereby improving the accuracy of characterization, and thus improving experimental precision and success rate. On the other hand, the cover plate 20 can be transferred synchronously with the box body 10, and the opening and closing of the cover plate 20 is easier, which can improve the ease of use of the sample box 100 and facilitate the rapid retrieval and transfer of samples.

[0079] In other words, the sample box 100 of this application embodiment can make the cryo-transfer process simple, fast, and highly stable and reliable. It can reduce the probability of mechanical and chemical damage to the sample, ensure the integrity of the sample, especially sensitive samples, and improve the success rate and accuracy of characterization experiments.

[0080] According to some embodiments of this application, the box body 10 has a first surface a, which is spaced apart from the bottom wall of the box body 10, and a sample groove 11 extending toward the bottom wall is provided on the first surface a. A limiting part 12 is formed on the first surface a and / or the inner side wall of the box body 10.

[0081] The box body 10 has a first surface a formed above the bottom wall, the first surface a is spaced apart from the bottom wall, and a sample groove 11 extending toward the bottom wall is formed on the first surface a. The side wall of the box body 10 and the first surface a define an accommodating space above the first surface a. A limiting part 12 is formed above the first surface a. The limiting part 12 can be connected to the first surface a and extends toward the opening 13 of the box body 10 relative to the first surface a. Alternatively, the limiting part 12 can be formed on the inner side wall of the box body 10 and extends toward the interior of the accommodating space to form a limiting part 12 located above the first surface a and closer to the cover plate 20 relative to the first surface a.

[0082] For example, the limiting part 12 may be connected only to the first surface a, the limiting part 12 may be connected only to the side wall of the box body 10, or the limiting part 12 may be connected to both the first surface a and the side wall of the box body 10.

[0083] Therefore, by setting a limiting part 12 located in the accommodating space of the box body 10 and above the sample slot 11, the cover plate 20 is vertically limited. The limiting part 12 is used to connect and fix at least one of the first surface a or the side wall of the box body 10. The position of the limiting part 12 is more reasonable, and the structural stability of the limiting part 12 is higher. This can improve the limiting effect of the limiting part 12 on the box body 10, thereby further reducing the probability of the cover plate 20 causing the carrier mesh 200 to deform and improving the reliability of the sample box 100.

[0084] Combination Figures 1-5 as well as Figure 7 As shown, according to some embodiments of this application, the limiting part 12 is constructed as a limiting boss, and the limiting boss is at least connected to the inner sidewall of the box 10, or the limiting part 12 is constructed as a limiting post, and at least one end of the limiting post is connected to the first surface a.

[0085] In other words, in some embodiments, the limiting part 12 is constructed as a limiting boss, the orthographic projection outline of the limiting boss facing the first surface a is consistent with the outer outline of the box 10, and the outer surface of the limiting boss is connected to the inner sidewall, the inner surface defines an annular area to facilitate the placement and removal of samples and the carrier mesh 200, and the upper surface of the limiting boss is at a certain distance from the opening 13 to accommodate at least a portion of the cover plate 20, the lower surface of the limiting boss can be spaced apart from the first surface a, or the lower end of the limiting boss is connected to the first surface a, so as to achieve vertical limiting of the cover plate 20 by the limiting boss. In other embodiments, the limiting part 12 is constructed as a plurality of limiting posts, the lower end of the limiting posts is connected to the first surface a, and the upper end of the limiting posts is at a certain distance from the opening 13 to accommodate at least a portion of the cover plate 20.

[0086] In this way, by constructing the limiting part 12 as a limiting boss or a limiting post, the limiting part 12 can limit the cover plate 20. The force distribution of the limiting boss is more uniform, which can improve the limiting reliability and stability of the cover plate 20. In the embodiment where the limiting post is used for limiting, the limiting post is easier to process and uses less material than the limiting boss, which can reduce the processing difficulty of the box body 10 and reduce material costs.

[0087] Combination Figure 2 , Figure 3 as well as Figure 4As shown, according to some embodiments of this application, the end of the limiting part 12 away from the first surface a defines a second surface b spaced apart from the first surface a, and a receiving cavity is formed between the first surface a and the second surface b. The receiving cavity is used to receive the freezing medium so that the sample and the carrier mesh 200 are both immersed below the surface of the freezing medium.

[0088] In other words, the limiting part 12 defines the second surface b, which divides the accommodating space into an accommodating cavity and an accommodating part. The accommodating cavity is located below the accommodating part, and the sample slot 11 is located below the accommodating cavity. The sample slot 11 is used to place the carrier net 200 and the sample, while the accommodating cavity and the sample slot 11 are also used to accommodate a freezing medium (such as liquid nitrogen). The accommodating part is used to accommodate the cover plate 20.

[0089] In this way, by setting up the accommodating cavity, the space volume inside the box 10 for accommodating the freezing medium is larger, which can store more freezing medium, extend the freezing time of the sample, and improve the preservation effect of the sample. Moreover, the freezing medium can completely wet the sample and the grid 200, and can also achieve the isolation effect of isolating the sample and the grid 200 from the air, which can further reduce the probability of chemical damage to the sample, such as chemical reaction and deterioration, thereby further improving the preservation effect of the sample, improving the accuracy of characterization, and improving the success rate and experimental precision of subsequent experiments.

[0090] See Figure 2 , Figure 5 as well as Figure 7 As shown, according to some embodiments of this application, there are multiple sample slots 11, and each sample slot 11 is constructed as a rhomboid slot.

[0091] It is understandable that the sample slot 11 is constructed as a diamond-shaped slot, and the carrier mesh 200 is placed along the long diagonal of the diamond-shaped slot. The short diagonal can provide space for tweezers to pick up and store the carrier mesh 200. The thickness of the carrier mesh 200 is generally low. The diamond-shaped slot can effectively reduce the difficulty of picking up and placing the ultra-thin carrier mesh 200.

[0092] This reduces the difficulty of picking up and placing the carrier 200, improves the convenience of picking up and placing the carrier 200 and the sample, and also reduces the manufacturing difficulty of the sample slot 11, making it easier to demold the box 10.

[0093] like Figure 2 , Figure 5 and Figure 7 As shown, according to some embodiments of this application, at least two opposing sidewall surfaces of the sample groove 11 are constructed as a first inclined surface c.

[0094] That is, the sample cell 11 has four sidewalls, which are opposite to each other in pairs, and at least two of the opposite sidewall surfaces are constructed as the first inclined surface. c means that two or all four of the four sidewalls are constructed as inclined surfaces.

[0095] Understandably, to prevent deformation (e.g., bending) of the carrier 200 and to facilitate easy and convenient placement and removal of the carrier 200 within the sample cell 11, the sample cell 11 is often larger than the carrier 200. This makes it difficult to fix the carrier 200 within the sample cell 11, easily causing it to tilt towards the side where the sample is adsorbed or deposited, increasing the difficulty of handling the carrier 200. Furthermore, during the handling process, the carrier 200 is prone to deformation or even puncture of its coating, increasing the probability of damage. The deformation and damage of the carrier 200 will also affect subsequent sample loading and characterization, such as the inability to place the carrier 200 flat, affecting electron beam focusing, leading to a decrease in image resolution, and ultimately affecting the accuracy of characterization.

[0096] Based on this, by setting the first inclined surface c, the upper opening of the sample groove 11 can be larger than the lower opening, thereby guiding and fixing the carrier mesh 200. Under the guidance of the first inclined surface c, the carrier mesh 200 can gradually move and be fixed in the sample groove 11, which can reduce the probability of the carrier mesh 200 tipping over. This not only reduces the difficulty of picking up the carrier mesh 200, but also reduces the probability of puncturing the coating of the carrier mesh 200 and causing damage to the carrier mesh 200 during the picking process, resulting in less impact on subsequent sample loading and higher characterization accuracy.

[0097] Furthermore, in the embodiment where all four sidewalls are constructed as first inclined surfaces c, under the premise of achieving the same technical effect as two first inclined surfaces c, the carrier mesh 200 only contacts the sample groove 11 through two points in its own tangential direction, resulting in a smaller contact area, a lower probability of stress deformation of the carrier mesh 200, a lower probability of physical damage to the carrier mesh 200, less impact on subsequent sample loading, and further improvement in characterization accuracy.

[0098] Understandably, by setting the first inclined surface c, the stability of the carrier 200 placed in the sample groove 11 can be improved, and the gripping accuracy can be improved. The sample groove 11 gradually narrows downward, which can limit the range of motion of the carrier 200 and can clamp and fix the carrier 200 on both sides of its thickness, reducing the tendency of the carrier 200 to tilt to one side of its own thickness, reducing the probability of the carrier 200 tipping over, and also reducing the frequency of adjusting the carrier 200 with tweezers. This can reduce the probability of puncturing the coating of the carrier 200 or the carrier 200 being bent. In addition, the upper opening size of the sample groove 11 is larger than the lower opening size, which can also provide sufficient space for tweezers to pick up and put down, reduce the difficulty of picking up and putting down the carrier 200, and improve the convenience of operation.

[0099] like Figure 3As shown, according to some embodiments of this application, the first inclined surface c is inclined relative to the vertical surface perpendicular to the first surface a, and the inclination angle of the multiple first inclined surfaces c is consistent. The two opposing first inclined surfaces c can make the sample groove 11 form an open opening 13 with the top end expanding outward relative to the bottom end. The angle between the first edge formed by the intersection of two adjacent first inclined surfaces c of the sample groove 11 and the line connecting the extension lines of the two edges on the long diagonal of the sample groove 11 is 20°-30°, and the groove depth of the sample groove 11 is 3.4mm-4.3mm.

[0100] Specifically, the extension of the edge forms a first included angle with the vertical plane that is perpendicular to the first surface a and coincides with the short diagonal of the sample groove 11, which ranges from 10° to 15°, and the angle of the first included angle is twice the angle of the first included angle α.

[0101] It is understandable that when the angle of the first included angle is greater than the upper limit of 15°, the guiding effect of the first inclined surface c will be reduced. When the angle of the first included angle is less than the lower limit of 10°, the groove depth needs to be set deeper, which will not only increase the overall size of the box 10, but also increase the distance between the bottom of the sample groove 11 and the first surface a, making it more difficult to pick up the mesh 200 in the sample groove 11. The more reasonable first included angle in this application can balance the guiding effect on the mesh 200 and the ease of picking up the mesh 200.

[0102] According to some embodiments of this application, the depth of the sample groove 11 is 3.4mm-4.3mm.

[0103] For example, the long diagonal of the sample groove 11 is 4 mm. When the top of the carrier mesh 200 (diameter 3.05 mm) is flush with the opening of the sample groove 11 (i.e., flush with the first surface a, and the distance between the top of the carrier mesh 200 and the first surface a is 0 mm), the included angle of the first edge is 30.7°. When the distance between the top of the carrier mesh 200 and the first surface a is 1 mm, the included angle of the first edge will decrease to 20.2°.

[0104] The 1mm distance is to account for the fact that if the mesh 200 is placed deeper (i.e., the distance between the mesh 200 and the opening is >1mm), it would be difficult to grasp the mesh 200 with tweezers due to the size of the sample groove 11 opening. Therefore, the angle between the extended lines of the first inclined surfaces c on both sides of the sample groove 11 should be controlled within the range of 20°-30° to ensure that the distance between the opening of the sample groove 11 and the mesh 200 placed in the sample groove 11 is less than 1mm.

[0105] Therefore, the initial design depth of the sample groove 11 is 3.4 mm, with a 0.07 mm gap reserved above the carrier mesh 200 and a 0.28 mm gap reserved below. When the angle between the extended lines of the first inclined surfaces c on both sides of the sample groove 11 is 30.7°, the corresponding groove depth is 3.4 mm. When the angle between the extended lines of the first inclined surfaces c on both sides of the sample groove 11 is 20.2°, the corresponding groove depth should be 4.3 mm.

[0106] Therefore, this application makes the angle range of the first edge angle more reasonable. Under the premise that the length of the long diagonal is fixed, the short diagonal can be set shorter, so that more sample slots 11 can be arranged in the first surface a. At the same time, the depth of the sample slots 11 can be made more reasonable. It can avoid the slot depth being too shallow, causing the carrier 200 to protrude from the first surface a, thereby reducing the probability of the cover plate 20 pressing against the carrier 200. It can also avoid the slot depth being too deep, causing the carrier 200 to be located too far below the first surface a. It can take into account the convenience of taking the carrier 200 out.

[0107] like Figure 1 , Figure 2 and Figure 5 As shown, according to some embodiments of this application, a connecting groove 14 is provided between adjacent sample cells 11 to allow multiple sample cells 11 to communicate with each other; as Figure 7 As shown, according to some embodiments of this application, a plurality of sample slots 11 are spaced apart on the first surface a.

[0108] Exemplary examples show that in the first and second embodiments of this application, the plurality of sample slots 11 are configured to be sequentially connected in the arrangement direction. In the third embodiment of this application, the plurality of sample slots 11 are configured to be spaced apart. Of course, the plurality of sample slots 11 can be configured as follows: Figure 7 The sequential arrangement shown can also be configured to make full use of the irregular arrangement of the space on the first surface a, but the multiple sample slots 11 are spaced apart.

[0109] Thus, in embodiments where sample cells 11 are independent storage spaces, the cryogenic media inside multiple sample cells 11 will not mix, reducing the probability of cross-contamination between multiple samples and improving characterization accuracy. In embodiments where there are multiple sample cells 11 that are interconnected, the liquid level of the cryogenic media in multiple sample cells 11 is more consistent, reducing the occurrence of situations where a sample cell 11 is not completely filled with cryogenic media or even has no cryogenic media at all. This improves the wetting and isolation effects of the samples and the carrier mesh 200 within the sample cells 11, reducing air exposure and subsequent chemical damage to the samples due to insufficient cryogenic media, and improving characterization accuracy, especially for highly water- and oxygen-sensitive samples.

[0110] like Figure 1 , Figure 2 and Figure 5 As shown, according to some embodiments of this application, the connecting groove 14 is used to connect the short diagonal corner regions of adjacent sample grooves 11.

[0111] Specifically, the sample cell 11 is constructed as a rhomboid groove, with a short diagonal and a long diagonal. The two corners connected by the short diagonal are called short diagonal corners, and the two corners connected by the long diagonal are called long diagonal corners. The two long diagonal corner areas are used to hold and limit the net 200 and the sample. The short diagonal corner area is further provided with a connecting groove 14. While improving the consistency of the liquid level in multiple sample cells 11, the opening space on the first surface a can be further widened. This makes it easier to pick up and put down the net 200 and the sample through the short diagonal corner area with tweezers. The tweezers can have more room to move, which can reduce the difficulty of picking up and putting down the net 200 and improve the convenience of picking up and putting down.

[0112] like Figure 2 As shown, according to some embodiments of this application, the first center line of the housing 10 sequentially passes through the short diagonals of a plurality of sample slots 11, such as... Figure 7 As shown, according to some other embodiments of this application, the short diagonals of adjacent sample cells 11 are arranged in parallel within the first surface a.

[0113] Therefore, multiple sample slots 11 can be connected sequentially, and the first center line of the box 10 can pass through the short diagonals of multiple sample slots 11 sequentially. Alternatively, multiple sample slots 11 can be disconnected, and the first center line of the box 10 can pass through the short diagonals of multiple sample slots 11 sequentially. This can make the load distribution within the box 10 more uniform, reduce the probability of the sample box 100 tipping over, improve the reliability and stability of sample and net 200 transfer, and improve the preservation integrity of net 200 and sample. In embodiments where the short diagonals of adjacent sample slots 11 are arranged parallel in the first plane, the sample slots 11 can be rotated at a certain angle relative to the second center line. During the process of picking up net 200, there is no need to rotate the wrist to align with net 200, making the picking process more convenient and reducing the probability of net 200 being punctured. This not only reduces the difficulty of picking up net 200, but also reduces the probability of physical damage to net 200 or sample during the picking process, thereby improving the transfer speed and success rate.

[0114] Combination Figure 1 and Figure 3 As shown, according to some embodiments of this application, at least one sidewall of the connecting groove 14 is constructed as a second inclined surface d.

[0115] Specifically, the connecting groove 14 is constructed as a V-shaped groove, and at least one of the two side walls of the connecting groove 14 is constructed as a V-shape, so that the top opening size of the connecting groove 14 is larger. The connecting groove 14 with a larger opening size is used to avoid the tweezers, which can give the tweezers more operating space and further reduce the difficulty of picking up and placing the net 200.

[0116] Combination Figure 1 , Figure 2 , Figure 7 as well as Figure 8 As shown, according to some embodiments of this application, two first anti-rotation limiting recesses 15 are provided on the outer periphery of the box body 10, the two first anti-rotation limiting recesses 15 are arranged opposite to each other, and a second anti-rotation limiting recess 21 corresponding to the first anti-rotation limiting recesses 15 is provided on the cover plate 20.

[0117] In other words, a pair of first anti-rotation limiting recesses 15 are provided on the outer periphery of the box body 10, and a pair of second anti-rotation limiting recesses 21 are provided on the outer periphery of the cover plate 20. The two symmetrical first anti-rotation limiting recesses 15 can reduce the local outer diameter of the box body 10, making it easier for tweezers to grip the outer peripheral wall of the box body 10 and pick up the sample box 100. It can also further reduce the difficulty of transporting the sample box 100. Both first anti-rotation limiting parts 12 can be used to cooperate with the positioning protrusions on the freezing worktable, making the positioning cooperation between the box body 10 and the freezing worktable easier and more convenient to operate. Furthermore, the first anti-rotation limiting recesses 15 can cooperate with the second anti-rotation limiting recesses 21 to limit the rotation of the cover plate 20, which can reduce the movement of the cover plate 20 relative to the box body 10, thereby improving the fixation stability and reliability of the box body 10 on the cover plate 20, reducing the probability of the cover plate 20 falling off, reducing the probability of sample contamination, and improving the accuracy of subsequent characterization.

[0118] like Figure 7 As shown, according to some embodiments of this application, the second centerline of the box body 10 passes through two first anti-rotation limiting recesses 15 in sequence, and the second angle formed by the long diagonal line connecting the sample groove 11 and the second centerline is 15°-30°.

[0119] In this way, the sample slot 11 can be rotated at a certain angle relative to the second center line, which can reduce the difficulty of picking up the carrier 200. During the process of picking up the carrier 200, there is no need to rotate the wrist, reducing the degree of hand twisting and thus reducing the probability of damage to the carrier 200.

[0120] Understandably, the lower limit of the second included angle is 15°, and angles less than 15° are unlikely to effectively reduce the difficulty of handling the carrier net 200. The upper limit of the second included angle is 30°, and angles greater than 30° would increase the area occupied by a single sample slot 11 on the first surface a, resulting in fewer sample slots 11 to be arranged for the same area, making it difficult to balance the number of sample slots 11. Furthermore, angles exceeding 30° are also unlikely to effectively reduce the difficulty of handling the carrier net.

[0121] According to some embodiments of this application, the height of the box 10 is H, the outer diameter of the box 10 is D, and the height-to-diameter ratio is less than or equal to one.

[0122] It should be noted that, in order to increase the storage space for the freezing medium, the depth of the sample tank 11 can be increased based on the above-mentioned size range. However, in order to ensure that the distance between the mesh 200 and the opening is less than 1 mm, the increase in depth should be concentrated in the space below the mesh 200.

[0123] Understandably, although the groove depth can extend to 5mm or deeper, the overall height of the box 10 must not exceed its diameter. Based on stability criteria, an H / D (height-to-diameter ratio) ≤ 1 minimizes the risk of the box 10 tipping over and ensures good stability.

[0124] Therefore, by making the height-to-diameter ratio of the box 10 less than or equal to 1, the probability of the box 10 tipping over can be reduced, thereby improving the reliability and stability of the sample box 100 in preserving the sample.

[0125] It is understandable that a handle 22 is also provided on the cover plate 20. The cover plate 20 is embedded in the box body 10. The handle 22 makes it easier to open and close the cover plate 20 on the box body 10. When a heavier cover plate 20 is used, the sealing performance of the cover plate 20 on the box body 10 is better. The handle 22 can be constructed as a rounded rectangle to improve the convenience of picking up.

[0126] like Figures 1-4 as well as Figure 8 As shown, in the first embodiment of this application, the interior of the box body 10 is provided with a plurality of interconnected sample slots 11, and the four side walls of the sample slots 11 are constructed as first inclined surfaces c, the two side walls of the connecting slots 14 are constructed as second inclined surfaces d, and the limiting part 12 is constructed as a limiting boss and is located above the first surface. The outer periphery of the box body 10 is formed with a first anti-rotation limiting recess 15, and the outer periphery of the cover plate 20 is formed with a second anti-rotation limiting recess 21.

[0127] like Figure 5 and Figure 6As shown, in the second embodiment of this application, the interior of the box 10 is provided with a plurality of interconnected sample slots 11, and the four side walls of the sample slots 11 are constructed as first inclined surfaces c, the two side walls of the connecting slots 14 are constructed as second inclined surfaces d, and the limiting part 12 is constructed as a limiting boss and is located above the first surface.

[0128] like Figure 7 and Figure 8 As shown, in the third embodiment of this application, the interior of the box 10 is provided with a plurality of spaced sample slots 11, and the four side walls of the sample slots 11 are constructed as first inclined surfaces c. The sample slots 11 are rotated at a certain angle relative to the box 10, that is, the long diagonal line of the sample slots 11 is rotated at an angle of 15°-30° relative to the second center line. The limiting part 12 is constructed as a limiting boss and is located above the first surface.

[0129] Other configurations and operations of the sample box 100 according to the embodiments of this application are known to those skilled in the art and will not be described in detail here.

[0130] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0131] Although embodiments of this application have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the claims and their equivalents.

Claims

1. A sample holder for accommodating samples for cryo-transmission electron microscopy and a grid (200), characterized in that, include: The box body (10) has an open opening (13) at one end. At least one sample slot (11) is provided inside the box body (10). The box body (10) also has a limiting part (12) which is provided adjacent to the open opening (13) relative to the sample slot (11). A cover plate (20) is provided over the opening (13), and at least a portion of the cover plate (20) extends into the box body (10) and is pushed against the limiting part (12) to be spaced apart from the sample slot (11).

2. The sample box according to claim 1, characterized in that, The box body (10) has a first surface (a) inside, the first surface (a) is spaced apart from the bottom wall of the box body (10), and a sample groove (11) extending toward the bottom wall is provided on the first surface (a). The limiting part (12) is formed on the first surface (a) and / or the inner sidewall of the box body (10).

3. The sample box according to claim 2, characterized in that, The limiting part (12) is constructed as a limiting boss, and the limiting boss is at least connected to the inner sidewall of the box body (10), or the limiting part (12) is constructed as a limiting post, and at least one end of the limiting post is connected to the first surface (a).

4. The sample box according to claim 2 or 3, characterized in that, The limiting part (12) defines a second surface (b) spaced apart from the first surface (a) at one end away from the first surface (a). A receiving cavity is formed between the first surface (a) and the second surface (b). The receiving cavity is used to receive the freezing medium so that the sample and the carrier net (200) are both immersed below the surface of the freezing medium.

5. The sample box according to claim 2, characterized in that, There are multiple sample slots (11), and each of the multiple sample slots (11) is constructed as a rhomboid slot.

6. The sample box according to claim 5, characterized in that, The sample groove (11) has at least two opposing sidewall surfaces constructed as a first inclined plane (c).

7. The sample box according to claim 6, characterized in that, The angle formed by the intersection of the adjacent first inclined planes (c) and the extension lines of the two edges located on the long diagonal of the sample groove (11) are intersected is the first edge angle, the angle of the first edge angle is 20°-30°, and the groove depth of the sample groove (11) is 3.4mm-4.3mm.

8. The sample box according to any one of claims 5-7, characterized in that, A connecting groove (14) is provided between adjacent sample cells (11) so that the multiple sample cells (11) can communicate with each other.

9. The sample box according to claim 8, characterized in that, The connecting groove (14) is used to connect the short diagonal corner areas of the adjacent sample groove (11).

10. The sample box according to claim 9, characterized in that, The first centerline of the box body (10) passes through the short diagonals of the plurality of sample slots (11) in sequence; or the short diagonals of adjacent sample slots (11) are arranged in parallel within the first surface (a).

11. The sample box according to claim 8, characterized in that, At least one sidewall of the connecting groove (14) is constructed as a second inclined surface (d).

12. The sample box according to any one of claims 5-7, characterized in that, Multiple sample slots (11) are spaced apart on the first surface (a).

13. The sample box according to claim 1, characterized in that, Two first anti-rotation limiting recesses (15) are provided on the outer periphery of the box body (10), and the two first anti-rotation limiting recesses (15) are arranged opposite to each other. A second anti-rotation limiting recess (21) corresponding to the first anti-rotation limiting recesses (15) is provided on the cover plate (20).

14. The sample box according to claim 13, characterized in that, The second centerline of the box body (10) passes through the two first anti-rotation limiting recesses (15) in sequence, and the second angle formed by the long diagonal line of the sample groove (11) and the second centerline is 15°-30°.

15. The sample box according to claim 1, characterized in that, The height of the box (10) is H, the outer diameter of the box (10) is D, and the height-to-diameter ratio is less than or equal to 1.

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