A reactor provided with a make-up device

Abstract

The utility model discloses a reactor with feeding device, including at least one reaction container and feeding device, and feeding device includes pneumatic generator and feeding container, and feeding container includes at least one storage container and at least one temporary storage container, and the direct communication of three between storage container, reaction container, pneumatic generator, and pneumatic generator communicates with temporary storage container, still including at least one valve, and the valve makes temporary storage container unable to communicate with storage container and reaction container simultaneously, the utility model discloses a reactor with feeding device utilizes the gas pressure generated by pneumatic generator as power source drive the flow of liquid, according to the amount of control increasing or reducing gas pressure directly control the amount of supplementary liquid, realize high accuracy's feeding, under the whole reactor keeps sealed state, carry out sterilization to gas can guarantee the sterile state inside whole reactor, realized simple, high -efficient feeding's while effectively reduce cost and operation difficulty.

Description

Technical Field

[0001] This utility model relates to the field of bioreactor feeding, and in particular to a reactor equipped with a feeding device. Background Technology

[0002] Small / micro bioreactors require the addition of various feed solutions to replenish nutrients during bio-fermentation. Existing methods include peristaltic pump feeding, peristaltic pump feeding combined with a balance, centrifugal pump feeding combined with a flow sensor / balance and flow valve, and manual feeding with a pipette. However, due to variations in pump tubing, feeding using a peristaltic pump is slow. Even when combined with a balance, the balance itself lacks sufficient precision, failing to improve feeding accuracy. Feeding using a centrifugal pump combined with a flow sensor / balance and flow valve not only struggles to achieve microliter accuracy but is also complex to operate. Feeding with a pipette requires manual operation, and the accuracy is highly dependent on the operator's skill. Furthermore, pipette feeding is limited to clean laminar flow environments, making it impractical for feeding reactors. Utility Model Content

[0003] To address the problems existing in the prior art, this utility model provides a reactor equipped with a feeding device.

[0004] A reactor equipped with a feeding device includes at least one reaction vessel and a feeding device. The feeding device includes a pressure generator and a feeding container. The feeding container includes at least one storage container and at least one temporary storage container. The storage container, the reaction vessel, and the pressure generator are not directly connected to each other. The pressure generator is connected to the temporary storage container.

[0005] It also includes at least one valve, with each temporary storage container corresponding to a valve, and the valve prevents the temporary storage container from being connected to the storage container and the reaction container at the same time.

[0006] Specifically, the pressure generated by the pressure generator is used to replenish the liquid in the storage container to the reaction vessel. The specific operation process is as follows: initially, there is liquid in the storage container and no liquid in the temporary storage container. The valve is controlled to connect the temporary storage container and the storage container. The pressure generator is activated to reduce the pressure in the temporary storage container. Under the action of the pressure difference, the liquid in the storage container enters the temporary storage container. When the liquid in the temporary storage container reaches the specified amount, the valve is switched to connect the temporary storage container to the reaction vessel. At this time, the pressure generator is activated to increase the pressure in the temporary storage container, and the liquid in the temporary storage container enters the reaction vessel, thus realizing the replenishment of the liquid.

[0007] Preferably, when multiple temporary storage containers and valves are provided, each temporary storage container is independent of the others, and each temporary storage container is connected to the air pressure generating device, and the capacity of each temporary storage container is 1 to 500 μl.

[0008] Specifically, when a reaction vessel needs to be replenished with multiple liquids, each temporary storage container is connected to the same reaction vessel to replenish the multiple liquids; when multiple reaction vessels need to be replenished simultaneously, each reaction vessel can be paired with a temporary storage container, and several temporary storage containers can be connected to storage containers (selected to be the same storage container or different storage containers depending on the type of liquid being replenished), and several temporary storage containers can be connected to a pressure generating device, which is used to replenish the liquids in multiple reaction vessels; depending on the specific situation of each reaction vessel, the above two structures can be combined to replenish each reaction vessel.

[0009] Preferably, the temporary storage container is connected to the air pressure generating device via an independent air pressure pipeline;

[0010] The pneumatic pipeline is equipped with a first multi-way valve, which is used to control the connection status between each temporary storage container and the pneumatic generator.

[0011] Specifically, the timing of refueling for multiple reaction vessels is not uniform. By setting up a first multi-way valve, the connection between each gas pressure pipeline can be controlled, thereby controlling the connection status between the temporary storage vessel and the gas pressure generator to achieve on-demand refueling.

[0012] Preferably, the pressure generator includes a pressure generating device, which is used to increase or decrease the pressure inside the temporary storage container;

[0013] The pressure generating device is connected to the temporary storage container via a pressure pipeline. The pressure generating device is also equipped with an exhaust port, which is connected to the pressure generating device via an exhaust pipeline.

[0014] The exhaust pipe and the air pressure pipe are equipped with a second multi-way valve, which controls that the exhaust port and the temporary storage container cannot be connected to the air pressure generating device at the same time.

[0015] Specifically, the air pressure generator needs to be calibrated or reset before its first use or after a period of use. Therefore, an exhaust pipe needs to be installed. When calibration or reset is required, the second multi-way valve is switched to connect the air pressure generator to the exhaust port for calibration or reset.

[0016] When multiple temporary storage containers are provided, the first multi-way valve and the second multi-way valve can be combined into one.

[0017] Preferably, the second multi-way valve is a solenoid valve.

[0018] Preferably, the air pressure generating device includes a syringe and a motor that drives the piston to move. The direction of the piston movement is controlled by the rotation direction of the motor. The working pressure of the syringe is -1.62 kPa to +1.62 kPa.

[0019] Specifically, the structure of the syringe allows for more precise control over the amount of gas injected into or extracted from the temporary storage container, thereby enabling precise control over the increase or decrease in gas pressure within the container. The amount of increase or decrease in gas pressure is directly related to the amount of liquid added.

[0020] Preferably, a filtration device is provided between the air pressure generator and the temporary storage container, the filtration device being used for sterilization and / or filtration.

[0021] Specifically, in order to maintain a sterile environment inside the reaction vessel, it is necessary to sterilize the gas.

[0022] Preferably, the valve is a rotary valve or a solenoid valve.

[0023] Preferably, both the feeding container and the reaction container are consumables.

[0024] Preferably, the valve is driven by a valve motor.

[0025] Compared with the prior art, the advantages of this utility model are:

[0026] The gas pressure generated by the gas pressure generator is used as a power source to drive the flow of the feed liquid. At the same time, the amount of feed liquid replenished can be directly controlled by controlling the increase or decrease of the gas pressure, which realizes high-precision feeding. Meanwhile, in the state of keeping the entire reactor sealed, only the gas needs to be sterilized to ensure the sterility of the entire reactor. This achieves simple and efficient feeding while effectively reducing costs and operating difficulty. Attached Figure Description

[0027] Figure 1 This is a schematic diagram of the structure of a reactor equipped with a feeding device provided by this utility model;

[0028] Figure 2 This is a schematic diagram of another embodiment of the reactor equipped with a feeding device provided by this utility model. Detailed Implementation

[0029] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0030] like Figure 1As shown, a reactor equipped with a feeding device includes at least one reaction vessel 80 and a feeding device. The feeding device includes a pressure generator and a feeding container. The feeding container includes at least one storage container 10 and at least one temporary storage container 20. The storage container 10, the reaction vessel 80, and the pressure generator are not directly connected to each other. The pressure generator is connected to the temporary storage container 20.

[0031] It also includes at least one valve 30, with a one-to-one correspondence between the temporary storage container 20 and the valve 30. The valve 30 prevents the temporary storage container 20 from being connected to the storage container 10 and the reaction container 80 at the same time.

[0032] The liquid material in the storage container 10 is replenished to the reaction vessel 80 by the air pressure generated by the air pressure generator. The specific operation process is as follows: initially, there is liquid material in the storage container 10 and no liquid material in the temporary storage container 20. The control valve 30 connects the temporary storage container 20 to the storage container 10. The air pressure generator is activated to reduce the air pressure in the temporary storage container 20. Under the action of the air pressure difference, the liquid material in the storage container 10 enters the temporary storage container 20. When the liquid material in the temporary storage container 20 reaches the specified amount, the valve 30 is switched to connect the temporary storage container 20 to the reaction vessel 80. At this time, the air pressure generator is activated to increase the air pressure in the temporary storage container 20, so the liquid material in the temporary storage container 20 enters the reaction vessel 80, realizing the replenishment of the liquid material.

[0033] When multiple temporary storage containers 20 and valves 30 are provided, each temporary storage container 20 is independent of each other, and each temporary storage container 20 is connected to the air pressure generating device. The capacity of each temporary storage container 20 is 1 to 500 μl.

[0034] like Figure 2 As shown, when a reaction vessel 80 needs to be replenished with multiple liquids, each temporary storage container 20 is connected to the same reaction vessel 80 to replenish multiple liquids.

[0035] When multiple reaction vessels 80 need to be replenished simultaneously, each reaction vessel 80 can be associated with a temporary storage container 20. Several temporary storage containers 20 are connected to the same or different storage containers 10 depending on the type of replenishing liquid. Several temporary storage containers 20 are connected to a pressure generating device, and the pressure generating device is used to replenish the liquid in multiple reaction vessels 80.

[0036] Depending on the specific circumstances of each reaction vessel 80, the aforementioned two structures can be combined to feed each reaction vessel 80.

[0037] The temporary storage container 20 is connected to the air pressure generating device via an independent air pressure pipeline;

[0038] The pneumatic pipeline is equipped with a first multi-way valve, which is used to control the connection status between each temporary storage container 20 and the pneumatic generator.

[0039] The timing of replenishment for multiple reaction vessels 80 is not uniform. By setting up a first multi-way valve, the connection between each gas pressure pipeline can be controlled, thereby controlling the connection between the temporary storage container 20 and the gas pressure generator to achieve on-demand replenishment.

[0040] The pressure generator includes a pressure generating device, which is used to increase or decrease the pressure inside the temporary storage container 20;

[0041] The air pressure generating device is connected to the temporary storage container 20 via an air pressure pipeline. The air pressure generating device is also equipped with an exhaust port 70, which is connected to the air pressure generating device via an exhaust pipeline.

[0042] A second multi-way valve is installed on the exhaust pipe and the air pressure pipe. The second multi-way valve controls the exhaust port 70 and the temporary storage container 20 to prevent them from being connected to the air pressure generating device at the same time.

[0043] The air pressure generator needs to be calibrated or reset before its first use or after a period of use. Therefore, an exhaust pipe needs to be installed. When calibration or reset is required, the air pressure generator is connected to the exhaust port 70 by switching the second multi-way valve to perform calibration or reset.

[0044] When multiple temporary storage containers 20 are provided, the first multi-way valve and the second multi-way valve can be combined into one.

[0045] The second multi-way valve is a solenoid valve.

[0046] The air pressure generating device includes a syringe 40 and a motor 50 that drives the piston 41 to move. The direction of movement of the piston 41 is controlled by the rotation direction of the motor 50. The working pressure of the syringe 40 is -1.62 kPa to +1.62 kPa.

[0047] The structure of the syringe 40 allows for more precise control over the amount of gas injected into or extracted from the temporary storage container 20, thereby enabling precise control over the increase or decrease in gas pressure within the temporary storage container 20. The amount of increase or decrease in gas pressure is directly related to the amount of liquid added.

[0048] A filter device 60 is provided between the air pressure generator and the temporary storage container 20. The filter device 60 is used for gas sterilization and / or filtration.

[0049] To maintain a sterile environment within the reaction vessel 80, sterilization and / or filtration are necessary.

[0050] Valve 30 is a rotary valve or a solenoid valve; the feeding container and the reaction container 80 are both consumables; the operation of valve 30 is driven by a valve motor.

Claims

1. A reactor equipped with a feeding device, comprising at least one reaction vessel and a feeding device, characterized in that, The feeding device includes a pressure generator and a feeding container. The feeding container includes at least one storage container and at least one temporary storage container. The storage container, reaction container, and pressure generator are not directly connected to each other. The pressure generator is connected to the temporary storage container. It also includes at least one valve, with each temporary storage container corresponding to a valve, and the valve prevents the temporary storage container from being connected to the storage container and the reaction container at the same time.

2. The reactor equipped with a feeding device according to claim 1, characterized in that, When multiple temporary storage containers and valves are provided, each temporary storage container is independent of the others, and each temporary storage container is connected to the air pressure generating device. The capacity of each temporary storage container is 1 to 500 μl.

3. The reactor equipped with a feeding device according to claim 2, characterized in that, The temporary storage container is connected to the air pressure generating device via a separate air pressure pipeline; The pneumatic pipeline is equipped with a first multi-way valve, which is used to control the connection status between each temporary storage container and the pneumatic generator.

4. The reactor equipped with a feeding device according to claim 1 or 3, characterized in that, The pressure generator includes a pressure generating device, which is used to increase or decrease the pressure inside the temporary storage container. The pressure generating device is connected to the temporary storage container via a pressure pipeline. The pressure generating device is also equipped with an exhaust port, which is connected to the pressure generating device via an exhaust pipeline. The exhaust pipe and the air pressure pipe are equipped with a second multi-way valve, which controls that the exhaust port and the temporary storage container cannot be connected to the air pressure generating device at the same time.

5. The reactor equipped with a feeding device according to claim 4, characterized in that, The second multi-way valve is a solenoid valve.

6. The reactor equipped with a feeding device according to claim 4, characterized in that, The pressure generating device includes a syringe and a motor that drives the piston. The direction of the piston's movement is controlled by the rotation direction of the motor. The working pressure of the syringe is -1.62 kPa to +1.62 kPa.

7. The reactor equipped with a feeding device according to claim 1, characterized in that, A filtration device is provided between the air pressure generator and the temporary storage container, and the filtration device is used for sterilization and / or filtration.

8. The reactor equipped with a feeding device according to claim 1, characterized in that, The valve is a rotary valve or a solenoid valve.

9. The reactor equipped with a feeding device according to claim 1, characterized in that, Both the feeding container and the reaction container are consumables.

10. The reactor equipped with a feeding device according to claim 1, characterized in that, The valve's operation is driven by a valve motor.

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