A low disturbance cell collection system

Abstract

The utility model discloses a low disturbance cell collection system, including the outer tube, the inner tube is arranged in the outer tube, the inner tube is covered and has the pipe cover, wherein, the bottom of inner tube is provided with the sedimentation cavity for the cell precipitation, and the sidewall of inner tube and located above the sedimentation cavity is provided with a plurality of drainage groove, and the baffle is inserted in the drainage groove. The utility model discloses a baffle is inserted in the drainage groove sealing, and the inner tube is placed in the outer tube and pours into the centrifugal liquid and covers the pipe cover, and then is placed into the centrifugal equipment and is centrifuged. After centrifugation, the liquid is layered, and the cell is precipitated in the sedimentation cavity, and the upper layer is liquid. At this time, only need to open the pipe cover, and the baffle is pulled up, and the bottom of drainage groove is opened, and the upper layer liquid will flow into the outer tube naturally, and the cell is steady and stays in the sedimentation cavity, and solid-liquid separation is realized easily. The whole process is simple to operate, and the complicated transfer or filtering step is not needed, so that the disturbance of human operation to the cell is reduced greatly, and the integrity and activity of the cell are protected to the maximum extent.

Description

Technical Field

[0001] This utility model relates to a low-disturbance cell collection system and belongs to the field of cell collection technology. Background Technology

[0002] In cell-related experiments, solid-liquid separation is a crucial step; however, traditional separation methods have many drawbacks. Commonly used methods, such as using a pipette for multiple aspirations to achieve solid-liquid separation, are not only cumbersome, requiring repeated and precise control of the pipette, consuming significant time and effort, but also susceptible to inaccurate aspiration volumes due to human error, affecting separation results. Another method is to pour the liquid directly from the separation flask. During pouring, the cell pellet is often not compacted and easily spills out with the liquid, leading to cell loss and compromising the accuracy and reliability of subsequent experiments. Furthermore, these traditional methods cause significant disturbance to the cells during operation, easily damaging cell viability and structure, which is detrimental to further cell culture and research. Therefore, a low-disturbance cell collection system is proposed. Utility Model Content

[0003] To address the aforementioned technical shortcomings, the purpose of this invention is to provide a low-disturbance cell collection system that improves experimental efficiency, reduces operational difficulty, and minimizes cell disturbance.

[0004] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: The present invention provides a low-disturbance cell collection system, comprising: outer tube; An inner tube, which is disposed inside the outer tube; A pipe cap, which is attached to the inner pipe; The inner tube has a sedimentation chamber at the bottom for settling cells, and multiple drainage channels are provided on the side wall of the inner tube above the sedimentation chamber. Baffles are inserted into the drainage channels to seal them.

[0005] Preferably, it also includes a ring, and the upper ends of the plurality of baffles are fixed on the ring; When the baffle is inserted into the drain tank, the ring is located at the inlet of the inner tube.

[0006] Preferably, the outer wall of the inner tube opening is provided with a first annular groove, the outer side of the ring is provided with a second annular groove, and the inside of the tube cover is symmetrically connected to two sliders by springs, each slider being provided with a first locking block and a second locking block; When the cap is placed on the inner tube, the first locking block and the second locking block are respectively engaged in the first annular groove and the second annular groove.

[0007] Preferably, the width of the first annular groove is greater than the width of the second annular groove; When the cap moves upward relative to the inner tube, the second locking block first pulls the ring upward, and then the first locking block pulls the inner tube upward.

[0008] Preferably, the upper part of the slider extends through the tube cover to the outside to form a handle.

[0009] Preferably, the inner tube has a shoulder on its upper part, the shoulder overlaps the upper contour of the outer tube, the cap is pressed onto the shoulder, and the cap is threadedly connected to the opening of the outer tube.

[0010] Preferably, a rubber sleeve is fixedly fitted onto the outer wall of the shoulder; When the inner tube is placed inside the outer tube, the rubber sleeve is located at the connection between the outer tube opening and the inner tube.

[0011] Preferably, the outer tube is provided with a support plate inside for supporting the inner tube.

[0012] Preferably, the inner wall of the inner tube is provided with a layer of filter paper.

[0013] Preferably, a sealing gasket for sealing is adhered to the outer contour of the baffle.

[0014] Compared with existing technologies: 1. This invention involves sealing a drain tank with a baffle, placing the inner tube inside the outer tube, pouring in the liquid to be centrifuged, and then capping the tube before centrifuging. After centrifugation, the liquid separates into layers, with cells settling in the sedimentation chamber and the upper layer being liquid. Simply open the tube cap, pull the baffle upwards to open the bottom of the drain tank, and the upper liquid will naturally flow into the outer tube, while the cells remain stably in the sedimentation chamber, easily achieving solid-liquid separation. The entire process is simple to operate, requiring no complex transfer or filtration steps, greatly reducing human disturbance to the cells and maximizing the protection of cell integrity and viability.

[0015] 2. This invention uses a ring to fix the upper part of multiple baffles, and creates an annular groove on the outer wall of the inner tube opening and the outside of the ring. This, combined with a slider with double locking blocks and a spring-loaded sliding connection inside the tube cap, makes installation more convenient and stable. When the tube cap is rotated to tighten, the double locking blocks engage with their corresponding annular grooves, and the tube cap and the upper end of the outer tube also clamp the inner tube's ring shoulder, ensuring overall structural stability during centrifugation. Solid-liquid separation after centrifugation is also very simple and efficient. When the tube cap is rotated upwards, the slider first drives the ring and baffles upwards via the second locking block, opening the lower end of the drainage channel to drain the liquid into the outer tube. Continuing to move the tube cap upwards, when the first locking block is at the top of the first annular groove, the tube cap, inner tube, and ring move upwards simultaneously, easily removing the inner tube and achieving solid-liquid separation where solid cells remain in the sedimentation chamber and liquid remains in the outer tube. This improves experimental efficiency and reduces operational difficulty and disturbance to cells. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is an overall sectional view of the present invention; Figure 3 For the present utility model Figure 2 Enlarged view of point A; Figure 4 This is a cross-sectional view of the outer tube, inner tube, and tube cap of this utility model; Figure 5 This is an exploded cross-sectional view of the outer tube, inner tube, and tube cap of this utility model. Figure 6 This is an exploded cross-sectional view of the inner tube, baffle, and ring of this utility model.

[0017] In the picture: 1. Outer tube, 2. Inner tube, 201. Sedimentation chamber, 202. Drainage trough, 203. Shoulder ring, 204. Filter paper, 205. First annular groove, 3. Tube cover, 4. Baffle, 5. Rubber sleeve, 6. Support plate, 7. Ring, 701. Second annular groove, 8. Spring, 9. Sliding block, 901. First locking block, 902. Second locking block, 10. Sealing gasket. Detailed Implementation

[0018] The present invention is illustrated below with specific embodiments, but these are not intended to limit the scope of the invention.

[0019] Example 1 like Figures 1-6 As shown, in this embodiment, a low-disturbance cell collection system is provided, including an outer tube 1; an inner tube 2 is disposed inside the outer tube 1; and a tube cap 3 is attached to the top of the inner tube 2. The inner tube 2 has a sedimentation chamber 201 at the bottom for settling cells. Multiple drainage channels 202 are provided on the side wall of the inner tube 2 above the sedimentation chamber 201. A baffle 4 is inserted into the drainage channel 202 to seal the drainage channel 202.

[0020] In use, insert the baffle 4 into the drain tank 202, then place the inner tube 2 into the outer tube 1, pour the liquid to be centrifuged into the inner tube 2, and cover the inner tube 2 with the tube cap 3. Place the device on the centrifuge equipment. At this time, the liquid to be centrifuged will be centrifuged in the inner tube 2. After centrifugation, the liquid will separate into layers, the cells will precipitate in the sedimentation chamber 201, and the liquid will be on the upper layer of the cells. Open the tube cap 3, and then pull the baffle 4 upward, causing the bottom of the drain tank 202 to open. The liquid will flow from the drain tank 202 into the outer tube 1, while the cells will remain in the sedimentation chamber 201 of the inner tube 2, thereby achieving solid-liquid separation.

[0021] The outer tube 1 has a support plate 6 inside to support the inner tube 2.

[0022] The inner wall of the inner tube 2 is provided with a layer of filter paper 204.

[0023] A sealing gasket 10 is adhered to the outer contour of the baffle 4 for sealing. The sealing gasket 10 is used to effectively seal the connection between the baffle 4 and the drain tank 202 to prevent liquid leakage.

[0024] Example 2 Based on Embodiment 1, this embodiment also includes a ring 7, and the upper ends of multiple baffles 4 are fixed on the ring 7; When the baffle 4 is inserted into the drain tank 202, the ring 7 is located at the opening of the inner tube 2.

[0025] The inner tube 2 has a first annular groove 205 on the outer wall of the tube opening, and a second annular groove 701 on the outer side of the ring 7. The tube cover 3 has two sliders 9 symmetrically connected by springs 8 inside, and each slider 9 is provided with a first locking block 901 and a second locking block 902. When the cap 3 is placed on the inner tube 2, the first locking block 901 and the second locking block 902 are respectively engaged in the first annular groove 205 and the second annular groove 701.

[0026] The width of the first annular groove 205 is greater than the width of the second annular groove 701; Installation process: The tube cap 3 contacts the thread on the outer tube 1. Rotating the tube cap 3 causes it to tighten continuously. The tube cap 3 moves downward, causing the first locking block 901 on the slider 9 to engage in the first annular groove 205 and the second locking block 902 to engage in the second annular groove 701. After the tube cap 3 is tightened, the tube cap 3 and the upper end of the outer tube 1 clamp and fix the annular shoulder 203 of the inner tube 2. Since the first annular groove 205 on the inner tube 2 is relatively wide, the first locking block 901 slides at the bottom of the first annular groove 205. In this state, centrifugation operation is achieved. After centrifugation, the solid-liquid separation process: Rotate the cap 3 to move it upward. During this process, the slider 9 will first move the ring 7 upward through the second locking block 902, causing the baffle 4 of the ring 7 to slide upward in the drain groove 202. The first locking block 901 located in the first annular groove 205 will also slide upward, and the lower end of the drain groove 202 will open, allowing the liquid in the inner tube 2 to drain into the outer tube 1. As the cap 3 continues to move upward, when the first locking block 901 located in the first annular groove 205 is at the upper part of the first annular groove 205, the cap 3, the inner tube 2, and the ring 7 move upward synchronously, thereby removing the inner tube 2 from the outer tube 1. The liquid is in the outer tube 1, and the solid cells are in the sedimentation chamber 201 of the inner tube 2, achieving solid-liquid separation.

[0027] The upper part of the slider 9 extends through the tube cap to the outside to form a handle 903. The handle 903 can drive the slider 9 to compress the spring 8 and slide, causing the locking block to disengage from the slot, thus separating the tube cap 3 from the inner tube 2 and the ring 7. In this way, after the inner tube 2 is separated, it is convenient for cell extraction.

[0028] A shoulder 203 is provided above the inner tube 2. The shoulder 203 overlaps the upper contour of the outer tube 1. The tube cap 3 is pressed onto the shoulder 203 and is connected to the opening of the outer tube 1 by thread engagement.

[0029] A rubber sleeve 5 is fixedly fitted onto the outer wall of the shoulder 203; When the inner tube 2 is placed inside the outer tube 1, the rubber sleeve 5 is located at the connection between the outer tube 1 and the inner tube 2. The rubber sleeve 5 between the inner tube 2 and the outer tube 1 provides resistance for the upward movement of the inner tube 2, which ensures that when the ring 7 moves upward with the baffle 4, the positions of the inner tube 2 and the outer tube 1 remain unchanged. In this way, when the inner tube 2 is inside the outer tube 1, the drain trough 202 opens first, and then the inner tube 2 separates from the outer tube 1.

[0030] Finally, it should be noted that the above embodiments are only used to illustrate and not limit the technical solutions of this utility model. Although this utility model has been described in detail with reference to the above embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to this utility model without departing from the spirit and scope of this utility model. Any modifications or partial substitutions should be covered within the scope of the claims of this utility model.

Claims

1. A low-disturbance cell collection system, characterized in that, include: Outer tube (1); Inner tube (2), which is disposed inside outer tube (1); The pipe cap (3) is attached to the inner pipe (2); The inner tube (2) has a sedimentation chamber (201) for settling cells at the bottom. Multiple drainage channels (202) are provided on the side wall of the inner tube (2) above the sedimentation chamber (201). A baffle (4) for sealing the drainage channel (202) is inserted into the drainage channel (202).

2. The low-disturbance cell collection system according to claim 1, characterized in that, It also includes a ring (7), and the upper ends of multiple baffles (4) are fixed on the ring (7); When the baffle (4) is inserted into the drain tank (202), the ring (7) is located at the opening of the inner tube (2).

3. The low-disturbance cell collection system according to claim 2, characterized in that, The inner tube (2) has a first annular groove (205) on the outer wall of the tube opening, and the ring (7) has a second annular groove (701) on the outside. The tube cover (3) has two sliders (9) symmetrically connected by springs (8) inside. Each slider (9) is provided with a first locking block (901) and a second locking block (902). When the cap (3) is placed on the inner tube (2), the first locking block (901) and the second locking block (902) are respectively engaged in the first annular groove (205) and the second annular groove (701).

4. The low-disturbance cell collection system according to claim 3, characterized in that, The width of the first annular groove (205) is greater than the width of the second annular groove (701); When the cap (3) moves upward relative to the inner tube (2), the second locking block (902) first pulls the ring (7) upward, and the first locking block (901) then pulls the inner tube (2) upward.

5. The low-disturbance cell collection system according to claim 3, characterized in that, The upper part of the slider (9) extends through the tube cover to the outside to form a handle (903).

6. The low-disturbance cell collection system according to claim 4, characterized in that, The inner tube (2) is provided with a shoulder (203) on its upper part. The shoulder (203) overlaps the upper contour of the outer tube (1). The tube cap (3) is pressed onto the shoulder (203) and is connected to the opening of the outer tube (1) by thread engagement.

7. The low-disturbance cell collection system according to claim 6, characterized in that, A rubber sleeve (5) is fixedly fitted onto the outer wall of the shoulder (203); When the inner tube (2) is placed inside the outer tube (1), the rubber sleeve (5) is located at the connection between the opening of the outer tube (1) and the inner tube (2).

8. The low-disturbance cell collection system according to claim 1, characterized in that, The outer tube (1) is provided with a support plate (6) for supporting the inner tube (2).

9. The low-disturbance cell collection system according to claim 1, characterized in that, The inner wall of the inner tube (2) is provided with a layer of filter paper (204).

10. A low-disturbance cell collection system according to claim 1, characterized in that, A sealing gasket (10) for sealing is adhered to the outer contour of the baffle (4).

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