A sample processing and spotting device for traditional Chinese medicine powder microscopic identification

Abstract

The utility model relates to a sample processing and spotting device for traditional Chinese medicine powder microscopic identification, which can effectively solve the problem of rapid detection in traditional Chinese medicine powder microscopic identification, and the technical solution is that a push rod assembly is arranged in the spotting device cylinder, a spotting port is arranged at the front end of the spotting device cylinder, a filter screen and a liquid leakage port matched with the spotting port are arranged at the front end of the spotting device cylinder, the push rod assembly comprises an outer push rod and an inner push rod, the outer push rod is composed of an outer push rod handle connected with an outer push rod middle column and a terminal part, the outer push rod middle column accommodates the inner push rod in the inner cavity, the front end of the inner push rod is provided with a convex rod, a sealing sheet I and a sealing sheet II are sequentially arranged on the convex rod from back to front, the spotting port is composed of an inner thread cylinder, an adjusting cylinder and a liquid outlet, the sample permeation, filtration and spotting functions are integrated in a closed system, the safety and convenience of operation are realized through the unique mechanical design, the ideal permeation effect of the sample can be ensured, the tablet preparation efficiency is improved by more than 10 times, and high-quality and efficient tablet preparation is realized.

Description

Technical Field

[0001] This utility model relates to the field of microscopic identification, and in particular to a sample processing and spotting device for microscopic identification of traditional Chinese medicine powder. Background Technology

[0002] Microscopic identification is a method that uses microscopic techniques to analyze traditional Chinese medicines in order to determine their variety and quality. Among them, powder microscopic identification is an important means of identifying the authenticity and quality of medicinal materials.

[0003] The 1977 edition of the Chinese Pharmacopoeia first stipulated the microscopic identification of medicinal materials. To date, the chloral hydrate permeabilization method remains the most commonly used sample preparation method and has been widely used since then. The specific method of chloral hydrate permeabilization is as follows: Take a small amount of the medicinal material powder, place it on a glass slide, add chloral hydrate solution, stir well with a dissecting needle, and gently heat it above an alcohol lamp flame until bubbles appear at the edges. Immediately stop heating and repeat 2-3 times to make the cell tissue transparent. Often, a small amount of dilute glycerin is added after permeabilization, followed by a coverslip.

[0004] The processing of powder samples directly affects the results of microscopic observation. However, there have long been five main problems with permeation preparation using chloral hydrate: 1. Current sample processing requires repeated sampling, placement, addition of chloral hydrate solution, stirring, lighting of an alcohol lamp, heating, cooling, addition of glycerol, and sealing. The process is complicated, time-consuming, and labor-intensive.

[0005] 2. When heating with a flame, it is difficult for operators to accurately judge the degree of permeation based solely on their subjective visual inspection. Inexperienced operators often fail to achieve the desired permeation effect. Insufficient permeation requires repeated operations, which is time-consuming and labor-intensive; boiling of the solution may cause excessive dissolution or destruction of the sample, affecting the permeation effect. Boiling also produces bubbles, which may interfere with microscopic observation and affect the clarity of the sample's tissue structure; overheating may dry out or even scorch the material.

[0006] 3. If there are a few large particles in the powder, these large particles may lift the coverslip, and the reagent cannot be evenly distributed in the space between the slide and the coverslip. The powder is easy to move on the slide, affecting the observation effect. Therefore, in actual operation, it is necessary to use tweezers to remove the large particles.

[0007] 4. The bubbles generated during heating of chloral hydrate solution originate from a combination of factors: the volatilization of thermal decomposition products, the release of dissolved gases, and the evaporation of water. Chloral hydrate is of low toxicity; when heated with an alcohol lamp during permeation, the temperature easily exceeds 98°C, causing it to decompose into trichloroacetaldehyde and water. If the temperature rises further, trichloroacetaldehyde may further volatilize or decompose, releasing chloroform. These volatile substances may escape as gases, forming bubbles. These toxic gases directly endanger the health of operators and pollute the environment. Chloral hydrate solution may contain dissolved air or other gases. As the temperature rises, gas solubility decreases, and the initially dissolved gases gradually precipitate out, forming bubbles. Water in the aqueous solution evaporates during heating, especially near boiling point, where water vapor forms larger bubbles.

[0008] 5. The open flame during the use of alcohol lamps poses a flammable and explosive hazard.

[0009] Especially in teaching experiments for the microscopic identification of traditional Chinese medicine (TCM), multiple students often perform chloral hydrate permeation slide preparation, leading to the aforementioned problems, which have long plagued teachers, students, and laboratory technicians. Microscopic identification is a mandatory test item in the pharmacopoeia of TCM materials and processed TCM slices. Microscopic identification techniques require high levels of professional skills and experience from operators. Currently, there is a severe shortage of relevant professionals, resulting in a predicament for microscopic identification. Furthermore, manual testing not only has a high error rate but is also extremely labor-intensive. With the maturity and widespread adoption of fully automated slide scanning microscopy systems, microscopic imaging of TCM powders is expected to achieve unattended, fully automated scanning and image analysis, enabling easy and efficient work. The rapid development of artificial intelligence makes it possible to establish an AI-based recognition system for TCM microscopic identification, potentially achieving low-cost, accurate, rapid, and real-time target detection of TCM in the future. The current shortcomings of chloral hydrate permeation slide preparation urgently need to be addressed. This method falls far short of achieving high-quality and efficient slide preparation and is a major bottleneck in achieving rapid detection in the microscopic identification of TCM powders.

[0010] Overcoming the above-mentioned defects has become a key issue that needs to be addressed in the microscopic slide preparation process. Therefore, it is urgent to design a sample processing and spotting device that is multifunctional, efficient, controllable, safe and environmentally friendly for the microscopic identification of traditional Chinese medicine powders. Utility Model Content

[0011] In view of the above situation and to overcome the shortcomings of the existing technology, the purpose of this utility model is to provide a sample processing and spotting device for microscopic identification of traditional Chinese medicine powder, which can effectively solve the problem of rapid detection in microscopic identification of traditional Chinese medicine powder.

[0012] The technical solution provided by this utility model includes a sampler cylinder, a pusher assembly, and a sample outlet. The sampler cylinder contains the pusher assembly, with a sample outlet at the front end. The front end of the sampler cylinder is equipped with a filter screen and a leakage port that match the sample outlet, together forming a liquid discharge control module. The pusher assembly includes an outer pusher and an inner pusher. The outer pusher consists of an outer pusher central column and an outer pusher handle connected to the end. The inner pusher is accommodated in the inner cavity of the outer pusher central column. The front end of the inner pusher is provided with a protruding rod, and sealing sheet I and sealing sheet II are sequentially installed on the protruding rod from back to front. The sample outlet consists of an internally threaded cylinder, an adjusting cylinder, and a liquid outlet. The inner wall of the internally threaded cylinder is provided with internal threads that match the external threads on the leakage port. The adjusting cylinder is sleeved outside the internally threaded cylinder, and a liquid outlet is provided at the front end of the adjusting cylinder. By rotating the adjusting cylinder, sealing or discharging of liquid can be achieved.

[0013] Furthermore, the sample applicator tube is a hollow cylindrical cavity.

[0014] Furthermore, the filter screen is made of high-precision stainless steel or polytetrafluoroethylene material, with a pore size of 100-200μm.

[0015] Furthermore, the rear part of the leakage port is a thin-walled hollow cylinder with external threads, which is connected to the internally threaded cylinder and axially penetrates to form a cavity. The front part is a thin-walled cone with six symmetrical leakage holes symmetrically opened on the side wall of the cone. The cavity is connected to the leakage holes.

[0016] Furthermore, the rear part of the adjusting cylinder is provided with an internal thread groove, and the front part of the internal thread cylinder is provided with an external thread ring that matches the internal thread groove. The rotating adjusting cylinder can move axially along the internal thread cylinder.

[0017] Furthermore, both sealing sheet I and sealing sheet II are circular. Sealing sheet I is fixedly installed at the front end of the middle column of the outer push rod, and the front end of sealing sheet I is attached to sealing sheet II through the locking block on the protrusion of the inner push rod.

[0018] Furthermore, the sealing sheet I and sealing sheet II are made of chemically resistant elastic material, and their outer diameter matches the inner diameter of the sample dispenser cylinder, thus forming a sliding seal.

[0019] Furthermore, the outer push rod handle is provided with positioning block I and positioning block II, which are used to engage with the lever on the inner push rod handle.

[0020] Furthermore, the inner push rod is coaxially nested in the internal cavity of the outer push rod central column, and can slide and rotate relative to the outer push rod central column. Its rear end is provided with an inner push rod handle, and a lever is provided on the inner push rod handle. The lever is used to selectively engage with positioning block I or positioning block II on the outer push rod handle, thereby limiting the rotational position of the inner push rod relative to the outer push rod.

[0021] Furthermore, a locking block is installed on the outer peripheral wall of the protruding rod, and an elastic ball is mounted on the locking block. The inner wall of the sealing plate II is provided with a locking groove that matches the locking block and a positioning groove that matches the elastic ball. The shape of the locking groove matches the locking block, allowing the locking block to be inserted axially. The shape and position of the positioning groove are designed to accommodate and lock the elastic ball after the sealing plate II rotates at a certain angle relative to the protruding rod, providing positioning and holding force.

[0022] The beneficial technical effects of this utility model are as follows: By integrating sample permeation, filtration, and spotting functions into a closed system, and through unique mechanical design, the system achieves operational safety and convenience, ensuring ideal sample permeation results and increasing slide preparation efficiency by more than 10 times. This enables high-quality and efficient slide preparation, providing crucial technical support for rapid detection in the microscopic identification of traditional Chinese medicine powders. Furthermore, it reduces indoor air pollution and avoids the fire risk associated with open flame heating. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0024] Figure 2 This is a schematic diagram of the push rod assembly structure of this utility model.

[0025] Figure 3 This is an exploded view of the overall structure of this utility model.

[0026] Figure 4 This is a schematic diagram of the cross-sectional structure of the sampling port of this utility model.

[0027] Figure 5 This is a schematic diagram showing the connection relationship between the central column of the external push rod and the sealing plate of this utility model.

[0028] Figure 6 This is an enlarged schematic diagram of the front end of the push rod assembly of this utility model.

[0029] Figure 7 This is a schematic diagram of the sealing sheet II of this utility model.

[0030] Figure 8 This is a schematic diagram of the sealing sheet II of this utility model.

[0031] Figure 9 This is a cross-sectional structural diagram of the sample dispensing cylinder and push rod assembly of this utility model.

[0032] Figure 10 This is a schematic diagram of the filter structure of this utility model.

[0033] In the attached diagram: 1. Sample dispenser cylinder; 2. Outer push rod; 3. Outer push rod handle; 4. Inner push rod handle; 5. Lever; 6. Positioning block I; 7. Positioning block II; 8. Internal threaded cylinder; 9. Adjusting cylinder; 10. Liquid outlet; 11. Internal threaded groove; 12. External threaded ring; 13. Leakage outlet; 14. Outer push rod center column; 15. Sealing plate I; 16. Sealing plate II; 17. Locking block; 18. Elastic ball; 19. Locking groove; 20. Positioning groove; 21. Protruding rod; 22. Inner push rod; 23. Filter screen. Detailed Implementation

[0034] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0035] refer to Figure 1-10 This utility model includes a sample dispensing cylinder, a push rod assembly, and a sample dispensing port. The sample dispensing cylinder 1 contains the push rod assembly, and the front end is equipped with a sample dispensing port. The front end of the sample dispensing cylinder 1 is provided with a filter screen 23 and a leakage port 13 that match the sample dispensing port, which together constitute a liquid discharge control module. The push rod assembly includes an outer push rod 2 and an inner push rod 22. The outer push rod 2 is composed of an outer push rod central column 14 and an outer push rod handle 3 connected to the end. The inner push rod 22 is accommodated in the inner cavity of the outer push rod central column 14. The front end of the inner push rod 22 is provided with a protruding rod 21. Sealing plate I 15 and sealing plate II 16 are sequentially installed on the protruding rod 21 from back to front. The sample dispensing port is composed of an internal threaded cylinder 8, an adjusting cylinder 9, and a liquid outlet 10. The inner wall of the internal threaded cylinder 8 is provided with an internal thread that matches the external thread on the leakage port 13. The adjusting cylinder 9 is sleeved outside the internal threaded cylinder 8. The front end of the adjusting cylinder 9 is provided with a liquid outlet 10. By rotating the adjusting cylinder 9, sealing or discharging of liquid can be achieved.

[0036] Furthermore, the sample applicator tube 1 is a hollow cylindrical cavity.

[0037] Furthermore, the filter screen 23 is made of high-precision stainless steel or polytetrafluoroethylene.

[0038] Furthermore, the filter screen 23 has a pore size of 100-200μm.

[0039] Furthermore, the rear part of the leakage port 13 is a thin-walled hollow cylinder with external threads, which is connected to the internally threaded cylinder 8 and forms a through cavity through it axially. The front part is a thin-walled cone with six symmetrical leakage holes symmetrically opened on the side wall of the cone. The through cavity is connected to the leakage holes.

[0040] Furthermore, the rear of the adjusting cylinder 9 is provided with an internal thread groove 11, and the front of the internal thread cylinder 8 is provided with an external thread ring 12 that matches the internal thread groove 11. The rotating adjusting cylinder 9 can move axially along the internal thread cylinder 8.

[0041] Furthermore, both sealing sheet I15 and sealing sheet II16 are circular. Sealing sheet I15 is fixedly installed at the front end of the outer push rod central column 14, and the front end of sealing sheet I15 is in contact with sealing sheet II16 via the locking block 17 on the protrusion 2 of the inner push rod 22.

[0042] Furthermore, the sealing sheet I15 and sealing sheet II16 are made of chemically resistant elastic material, and their outer diameter matches the inner diameter of the sampler cylinder 1, thus forming a sliding seal.

[0043] Furthermore, the outer push rod handle 3 is provided with positioning block I 6 and positioning block II 7, which are used to engage with the lever 5 on the inner push rod handle 4.

[0044] Furthermore, the inner push rod 22 is coaxially nested in the internal cavity of the outer push rod central column 14, and can slide and rotate relative to the outer push rod central column 14. Its rear end is provided with an inner push rod handle 4, and a lever 5 is provided on the inner push rod handle 4. The lever 5 is used to selectively engage with the positioning block I 6 or positioning block II 7 on the outer push rod handle 3, thereby limiting the rotational position of the inner push rod 22 relative to the outer push rod 2.

[0045] Furthermore, a locking block 17 is installed on the outer peripheral wall of the protruding rod 21, and an elastic ball 18 is mounted on the locking block 17. The inner wall of the sealing plate II 16 is provided with a locking groove 19 that matches the locking block 17 and a positioning groove 20 that matches the elastic ball 18. The shape of the locking groove 19 matches the locking block 17, allowing the locking block 17 to be inserted axially. The shape and position of the positioning groove 20 are designed to accommodate and lock the elastic ball 18 after the sealing plate II 16 rotates a certain angle relative to the protruding rod 21, providing positioning and holding force.

[0046] The sample applicator tube of this invention adopts a modified syringe-type structure, consisting of a hollow cylindrical cavity to accommodate the sample to be permeated, with a standard syringe plunger interface at the rear end to receive the external plunger 2. The inner wall of the sample applicator tube 1 is smooth, facilitating uniform sample distribution and flow. A filter screen 23 is installed at the front end of the sample applicator tube. This filter screen is made of high-precision stainless steel or polytetrafluoroethylene, possessing excellent chemical corrosion resistance and filtration performance. The filter screen is fixed to the inner wall of the tube by heat fusion. The filter screen has a pore size of 100-200μm, effectively filtering large particulate impurities in the sample and preventing large particles from lifting the coverslip after subsequent sealing, thus affecting the observation effect. The front end of the sample applicator tube 1 is fixedly connected to the drain port 13 as an integral structure. The drain port 13 has a rear part that is a thin-walled hollow cylinder with external threads, connected to the internally threaded cylinder 8 and axially penetrating to form a through cavity. The front part is a thin-walled cone with six symmetrical holes symmetrically opened on the side wall of the cone. The through cavity communicates with the drain port.

[0047] The liquid outlet comprises an internally threaded cylinder 8, an adjusting cylinder 9, and a liquid outlet 10. The inner wall of the internally threaded cylinder 8 has spiral patterns that match the external threads on the leakage outlet 13. The adjusting cylinder 9 is fitted around the internally threaded cylinder 8 and matches the external threaded ring 12 on the internally threaded cylinder 8 via its rear internally threaded groove 11. By rotating the adjusting cylinder 9, it can move axially along the internally threaded cylinder 8, and its end has a liquid outlet 10 to control the liquid flow rate. When the adjusting cylinder 9 is tightened backward, its front inner wall presses against the front conical end of the leakage outlet 13, sealing the liquid outlet 10 and closing the liquid outlet channel. When the adjusting cylinder 9 is rotated forward at a certain angle, a gap is created between its front end and the front end of the leakage outlet 13, opening the liquid outlet 10. Liquid flows out through the leakage hole and gap from the liquid outlet 10. The larger the rotation angle, the larger the gap, and the corresponding increase in flow rate. This design achieves precise liquid sampling control and reliable on / off functionality.

[0048] The outer push rod handle 3 is provided with positioning block I 6 and positioning block II 7. Connected to the outer push rod handle 3 is the outer push rod central column 14, which is a hollow tubular structure. A circular sealing plate I 15 is fixedly installed at the front end of the outer push rod central column 14. The sealing plate I 15 is preferably made of a chemically resistant elastic material, and its outer diameter matches the inner diameter of the sample applicator cylinder 1, forming a sliding seal.

[0049] The inner push rod 22 is coaxially nested within the internal cavity of the outer push rod central column 14, allowing it to slide and rotate relative to the outer push rod central column 14. Its rear end is provided with an inner push rod handle 4. A lever 5 is provided on the inner push rod handle 4. The lever 5 is used to selectively engage with positioning block I 6 or positioning block II 7 on the outer push rod handle 3, thereby limiting the rotational position of the inner push rod 22 relative to the outer push rod 2. When the lever 5 engages with different positioning blocks, the inner push rod 22 rotates 90° around its central axis relative to the outer push rod central column 14. Its front end is provided with a protruding rod 21. A locking block 17 and an elastic ball 18 are mounted on the protruding rod 21.

[0050] The sealing plate II 16 is an independent circular component, preferably made of polytetrafluoroethylene or other high-temperature and corrosion-resistant rigid or semi-rigid materials. Its outer diameter matches the inner diameter of the sample applicator cylinder 1. A slot 19 and a positioning groove 20 are formed on the inner wall of the sealing plate II 16. The shape of the slot 19 matches the shape of the locking block 17, allowing the locking block 17 to be inserted axially. The shape and position of the positioning groove 20 are designed to accommodate and lock the elastic ball 18 after the sealing plate II 16 rotates a certain angle relative to the protruding rod 21, providing positioning and holding force. Through this connection method, the relative angle between the sealing plate II 16 and the sealing plate I 15 is determined by the rotational position of the inner push rod 22 relative to the outer push rod 2.

[0051] The operation of this utility model is as follows: First, assemble the push rod assembly. Insert the inner push rod 22 into the outer push rod center column 14, move the push rod 5 until it engages with the positioning block I 6, and then align the slot 19 of the sealing plate II 16 with the locking block 17 at the front end of the inner push rod 22, and slide it in axially. Rotate the sealing plate II 16 so that the elastic ball 18 is embedded and locked in the positioning groove 20, thus completing the fixation of the sealing plate II 16. At this time, the lever is in the first position, the vent holes of the sealing plate II 16 and the sealing plate I 15 are aligned, and the device is in the "permeable venting state".

[0052] Next, assemble the sample dispenser cylinder and the outlet. Tighten the internal threaded cylinder 8 onto the external thread at the rear of the outlet 13, and tighten the adjusting cylinder 9.

[0053] Next, sample addition and permeation are performed. The sample to be permeated is added into the cavity through the opening at the rear end of the sample applicator tube 1. The sample volume is approximately 1 / 3 to 1 / 2 of the main container volume. The sample applicator is placed in the heating device, and the heating temperature is controlled at 60-80℃ for 5-10 minutes. During the heating process, the device is in a "permeation and venting state". Air bubbles are discharged through the channel formed by the overlapping of the vent holes of sealing plate II16 and sealing plate I15, which avoids sample overflow or uneven permeation caused by air bubble accumulation.

[0054] Next, switch to the sealed state. After heating is complete, remove the spotting device from the heating device. First, push the push rod until the sealing plate II16 is flush with the sample liquid surface to expel residual gas. Then, move the push rod 5 to engage with the positioning block II7. This operation causes the inner push rod 22 to rotate 90° relative to the outer push rod 2, which in turn causes the sealing plate II16 to rotate 90° relative to the sealing plate I15. At this time, the vent holes of the two sealing plates are misaligned and aligned, that is, the vent holes block each other, and the device is in a sealed state.

[0055] Next, the sample is dispensed. The outlet 10 of the dispensing device is aligned with the glass slide, and the adjusting cylinder 9 is slowly rotated forward at a certain angle to open the dispensing channel. By pushing the push rod, the outer push rod handle 3 is pushed, causing the entire push rod assembly to slide forward within the dispensing cylinder 1. The sealing plate II 16 pushes the permeated liquid, which sequentially passes through the filter screen, the through cavity and leakage hole of the leakage port 13, and the gap formed between the adjusting cylinder 9 and the front end of the leakage port 13, and is evenly discharged from the outlet 10, achieving precise sample dispensing.

[0056] Finally, close and complete the process. After the sample is dispensed, tighten the adjusting cylinder 9 backwards to close the outlet 10 and prevent liquid leakage. Relevant parts can be disassembled for cleaning or replacement.

[0057] This invention effectively solves the problems encountered in current chloral hydrate permeation slide preparation methods, ensuring ideal permeation effects and increasing slide preparation efficiency by more than 10 times. This results in high-quality and efficient slide preparation, providing crucial technical support for rapid detection of traditional Chinese medicine powders under microscopic imaging. Furthermore, it reduces indoor air pollution, avoids the fire risk associated with open flame heating, and is convenient and safe to use. It effectively solves the problem of low-cost, accurate, and rapid identification of traditional Chinese medicine powders, possessing practical application value.

Claims

1. A sample processing and spotting device for microscopic identification of traditional Chinese medicine powder, characterized in that, The system includes a sample dispensing cylinder, a push rod assembly, and a sample dispensing port. The sample dispensing cylinder (1) contains the push rod assembly, and the front end is equipped with a sample dispensing port. The front end of the sample dispensing cylinder (1) is provided with a filter screen (23) and a leakage port (13) that match the sample dispensing port, which together constitute the liquid dispensing control module. The push rod assembly includes an outer push rod (2) and an inner push rod (22). The outer push rod (2) is composed of an outer push rod central column (14) and an outer push rod handle (3) connected to the end. The inner push rod (22) is accommodated in the inner cavity of the outer push rod central column (14). The front end of the inner push rod (22) is... The end is provided with a protruding rod (21), and sealing plate I (15) and sealing plate II (16) are sequentially installed on the protruding rod (21) from back to front. The sampling port is composed of an internal threaded cylinder (8), an adjusting cylinder (9) and a liquid outlet (10). The inner wall of the internal threaded cylinder (8) is provided with an internal thread that matches the external thread on the leakage port (13). The adjusting cylinder (9) is sleeved outside the internal threaded cylinder (8). The front end of the adjusting cylinder (9) is provided with a liquid outlet (10). By rotating the adjusting cylinder (9), the liquid can be sealed or discharged.

2. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The sample applicator tube (1) is a hollow cylindrical cavity.

3. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The filter screen (23) is made of high-precision stainless steel or polytetrafluoroethylene material with a pore size of 100-200μm.

4. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The rear part of the drain port (13) is a thin-walled hollow cylinder with external threads, which is connected to the internal threaded cylinder (8) and forms a through cavity through the axis. The front part is a thin-walled cone with six symmetrical drain holes symmetrically opened on the side wall of the cone. The through cavity is connected to the drain holes.

5. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The adjusting cylinder (9) is provided with an internal thread groove (11) at the rear, and the internal thread cylinder (8) is provided with an external thread ring (12) that matches the internal thread groove (11) at the front. The rotating adjusting cylinder (9) can move axially along the internal thread cylinder (8).

6. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, Both sealing sheet I (15) and sealing sheet II (16) are circular. Sealing sheet I (15) is fixedly installed at the front end of the middle column (14) of the outer push rod. The front end of sealing sheet I (15) is attached to sealing sheet II (16) through the locking block (17) on the protrusion (21) of the inner push rod (22).

7. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The sealing sheet I (15) and sealing sheet II (16) are made of chemically resistant elastic material, and their outer diameter matches the inner diameter of the sampler cylinder (1) to form a sliding seal.

8. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The outer push rod handle (3) is provided with positioning block I (6) and positioning block II (7) to engage with the lever (5) on the inner push rod handle (4).

9. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The inner push rod (22) is coaxially nested in the inner cavity of the outer push rod middle column (14), and can slide and rotate relative to the outer push rod middle column (14). Its rear end is provided with an inner push rod handle (4), and a lever (5) is provided on the inner push rod handle (4).

10. The sample processing and spotting device for microscopic identification of traditional Chinese medicine powder according to claim 1, characterized in that, The outer peripheral wall of the protruding rod (21) is equipped with a locking block (17), and an elastic ball (18) is mounted on the locking block (17). The inner wall of the sealing sheet II (16) is provided with a locking groove (19) that matches the locking block (17) and a positioning groove (20) that matches the elastic ball (18).

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