A detection card for detecting content of jujuboside A in traditional Chinese medicinal materials

Abstract

The utility model relates to detection card technical field, and disclose a detection card of detecting the content of zizyphi spinae-rosae semen in traditional Chinese medicinal material, including upper card shell and lower card shell, the bottom of inside of lower card shell is installed with test paper fixed card shell no.

Description

Technical Field

[0001] This utility model relates to the field of detection card technology, specifically a detection card for detecting the content of jujube seed saponin A in traditional Chinese medicine. Background Technology

[0002] Some traditional Chinese medicinal herbs contain jujube seed saponin A, which has a variety of significant pharmacological activities, including sedation, sleep improvement, anti-oxidation, and protection of myocardial cells. Under current technology, its content is detected by a test card.

[0003] Current detection cards typically use colloidal gold-labeled antibodies and consist of a base plate, NC membrane, sample pad, conjugate pad, absorbent paper, and card casing. The amount of antibody can be determined through colorimetric results.

[0004] However, in actual operation, the inventors found the following problems: during the process of combining and installing the cartridge and the test paper, if the test paper shifts and the sample drop hole and observation hole are not aligned, the upper and lower cartridges need to be pried up manually, which is extremely inconvenient and can easily cause hand injuries during the prying process.

[0005] Based on this, the present invention provides a detection card for detecting the content of jujube seed saponin A in traditional Chinese medicine. Utility Model Content

[0006] To address the shortcomings of existing technologies, this utility model provides a detection card for detecting the content of jujube seed saponin A in traditional Chinese medicine materials. It has the advantages of convenient installation and disassembly with a snap-fit ​​design, thus solving the problems mentioned in the background technology.

[0007] This utility model provides the following technical solution: a detection card for detecting the content of jujube seed saponin A in traditional Chinese medicine, comprising an upper card shell and a lower card shell. A test strip fixing card shell is installed at the bottom of the lower card shell. A sample pad is abutted inside the test strip fixing card shell. A gold-labeled pad is fixedly connected to the side of the sample pad. An NC membrane is fixedly connected to the side of the gold-labeled pad. The middle portion of the NC membrane has a detection line T coated with jujube seed saponin A antigen and a control line C coated with goat anti-mouse secondary antibody. An absorbent pad is fixedly connected to the side of the NC membrane. Fixing plates are fixedly connected to both sides of the upper and lower card shells. Two fixing sleeves are fixedly installed on the side of the fixing plates. Rubber elastic beads are disposed inside both fixing sleeves. A pressing column is attached to the top of each rubber elastic bead. A locking protrusion is fixedly connected to the top of the pressing column. A C-shaped locking block is attached to the outer wall of the locking protrusion.

[0008] Preferably, the diameter of the cavity inside the fixed sleeve is greater than the diameter of the rubber elastic bead, and the inner wall of the fixed sleeve is in contact with the outer wall of the pressing column.

[0009] Preferably, the diameter of the cavity inside the fixing sleeve is larger than the diameter of the rubber elastic bead, and the inner wall of the fixing sleeve fits against the outer wall of the pressing column.

[0010] Preferably, the upper casing has an observation window located at the center.

[0011] Preferably, a second test paper fixing clip is installed inside the lower clip, and an absorbent pad is abutted against the inner side of the second test paper fixing clip.

[0012] Preferably, the upper and lower retainers cooperate to form a hollow cuboid, the length and width of the upper and lower retainers are the same, and the gold pad is coated with colloidal gold particles adsorbed with anti-jujube seed saponin A monoclonal antibody or latex microspheres (or Yingguang microspheres) chemically bonded with anti-jujube seed saponin A monoclonal antibody.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] This test card for detecting the content of jujube seed saponin A in traditional Chinese medicine uses two sets of locking protrusions in contact with the top and bottom of the C-shaped locking block cavity. During the contact process, the pressing column fixedly connected to the bottom of the locking protrusion squeezes the rubber elastic bead inside the fixed shell at the top of the fixed plate. As a result, the top of the locking protrusion is in a sliding state that fits against the C-shaped locking block cavity until the locking protrusion and the slot opened in the C-shaped locking block come into contact and fit together. The rubber elastic bead loses its resistance. The two sets of C-shaped locking blocks can be used to install and remove the upper and lower locking shells. During the entire operation, the shells can be quickly separated and the position of the test strip can be adjusted when the internal test strip shifts. This avoids the need to pry the shells with fingernails or tools, reducing the difficulty of disassembly. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the overall structure of the device of this utility model;

[0016] Figure 2 This utility model Figure 1 A schematic diagram of a partial structure;

[0017] Figure 3 This utility model Figure 1 A schematic diagram of the lower retaining structure;

[0018] Figure 4 This utility model Figure 1 A schematic diagram of the bottom structure of the upper casing;

[0019] Figure 5 This utility model Figure 2 A schematic diagram of a partial structure;

[0020] Figure 6 This is a schematic diagram of the internal structure of the fixed sleeve of this utility model.

[0021] In the diagram: 1. Upper clamping shell; 101. Observation window; 102. Sample drop hole; 103. Conical drain shell; 104. Drain tube; 2. Lower clamping shell; 201. Test paper fixing clamping shell one; 202. Sample pad; 203. Gold label pad; 204. NC membrane; 205. Detection line T; 206. Quality control line C; 207. Absorbent pad; 208. Test paper fixing clamping shell two; 3. C-type locking block; 301. Pressing column; 302. Fixing sleeve; 303. Fixing plate; 304. Clamping protrusion; 305. Rubber elastic bead. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] Please see Figure 1-6A detection card for detecting the content of jujube seed saponin A in traditional Chinese medicine includes an upper card shell 1 and a lower card shell 2. A test strip fixing card shell 201 is installed at the bottom inside the lower card shell 2. A sample pad 202 is abutted inside the test strip fixing card shell 201. A gold label pad 203 is fixedly connected to the side of the sample pad 202. An NC membrane 204 is fixedly connected to the side of the gold label pad 203. The middle portion of the NC membrane 204 has a detection line T205 coated with jujube seed saponin A antigen and a quality control line C206 coated with goat anti-mouse secondary antibody. An absorbent pad 207 is fixedly connected to the side of the NC membrane 204. Fixing plates 303 are fixedly connected to both sides of the upper card shell 1 and the lower card shell 2. Two [unclear characters] are fixedly installed on the side of the fixing plates 303. The fixed housing 302 has rubber elastic beads 305 inside each of the two fixed housings 302. The top of the rubber elastic beads 305 is attached to the pressure post 301. The top of the pressure post 301 is fixedly connected to the locking protrusion 304. The outer wall of the locking protrusion 304 is attached to the C-shaped locking block 3. The top and bottom of the cavity of the C-shaped locking block 3 and the two sets of locking protrusions 304 come into contact. During the contact process, the pressure post 301 fixedly connected to the bottom of the locking protrusion 304 compresses the rubber elastic beads 305 inside the fixed housing 302 on the top of the fixed plate 303. As a result, the top of the locking protrusion 304 is in a sliding state attached to the cavity of the C-shaped locking block 3 until the slot of the locking protrusion 304 and the C-shaped locking block 3 makes contact. When the rubber elastic bead 305 is in contact with the sample, it loses its binding force. Two sets of C-shaped locking blocks 3 can be used to install and remove the upper casing 1 and the lower casing 2. During the entire operation, the casings can be quickly separated to adjust the position of the test strip when it shifts internally, avoiding the need to pry it open with fingernails or tools, thus reducing the difficulty of disassembly. The upper casing 1 has an observation window 101 located in the center. Sample liquid is dripped in through the sample drop hole 102 and flows through the conical drain shell 103 and drain tube 104 onto the sample pad 202. Then, the jujube seed saponin A in the sample liquid competes with the jujube seed saponin A antigen on the detection line T205 for binding to the gold-labeled antibody. When the content of jujuboside A in the sample solution is high, more gold-labeled antibodies bind, resulting in less gold-labeled antibody binding to the detection line T205, and a lighter color at detection line T205. Conversely, when the content of jujuboside A in the sample solution is low, fewer gold-labeled antibodies bind, resulting in more gold-labeled antibody binding to the detection line T205, and a darker color at detection line T205. The goat anti-mouse antibody at the quality control line C206 can bind to all gold-labeled antibodies. The content level of jujuboside A can be determined by directly comparing the colors of the quality control line C206 and the detection line T205. A darker detection line T205 indicates a lower content of the target substance in the sample solution. The quantitative detection card can be read numerically by the color of the detection line T205 and the quality control line C206 using a reader; different values ​​represent different contents.

[0024] After completing the colorimetric operation described above, place the test card on an incubator. Use a pipette to draw 120 μL of the sample solution containing jujuboside A and add it vertically into the sample well of the test card. Cover the incubator and incubate for 10 minutes. Immediately after incubation, remove the test card and insert it into the liquid inlet of the reader, pointing outwards. Click "Start Test" to read the results. This test card can also detect the content of jujuboside A in samples such as traditional Chinese medicine, medicinal herbs, health products, and cosmetics for quantitative or qualitative analysis.

[0025] Please see Figure 2 , Figure 3 and Figure 4 The upper casing 1 has a sample drop hole 102, and a conical drain shell 103 is fixedly connected inside the sample drop hole 102. A drain pipe 104 is fixedly installed at the bottom of the conical drain shell 103, and the bottom end of the drain pipe 104 is in contact with the top of the sample pad 202. The lower casing 2 has a test paper fixing casing 208 installed inside, and an absorbent pad 207 is abutted against the inner side of the test paper fixing casing 208. The upper casing 1 and the lower casing 2 cooperate to form a hollow cuboid. The length and width of the upper casing 1 and the lower casing 2 are the same. The gold label pad 203 is sprayed with colloidal gold particles adsorbed with anti-jujube seed saponin A monoclonal antibody or latex microspheres (or luminescent microspheres) chemically bonded with anti-jujube seed saponin A monoclonal antibody.

[0026] Please see Figure 6 The diameter of the cavity inside the fixed sleeve 302 is greater than the diameter of the rubber elastic bead 305, and the inner wall of the fixed sleeve 302 is in contact with the outer wall of the pressing column 301.

[0027] Working principle: During operation, the top and bottom of the C-shaped locking block 3 cavity contact with two sets of locking protrusions 304. During contact, the pressing column 301 fixedly connected to the bottom of the locking protrusion 304 compresses the rubber elastic bead 305 inside the top fixing sleeve 302 of the fixing plate 303. The top of the locking protrusion 304 is then in a sliding state within the C-shaped locking block 3 cavity until the locking protrusion 304 and the slot of the C-shaped locking block 3 come into contact and adhere, at which point the rubber elastic bead 305 loses its resistance. The two sets of C-shaped locking blocks 3 allow for the installation and removal of the upper locking shell 1 and the lower locking shell 2. Sample liquid is dripped into the sample drop hole 102 and flows through the conical drain shell 103 and drain pipe 104 onto the sample pad 202. Then, the jujube seed saponin A in the sample liquid interacts with the detection... The jujube seed saponin A antigen on test line T205 competitively binds to the gold-labeled antibody. When the jujube seed saponin A content in the sample solution is high, more gold-labeled antibodies bind, resulting in less gold-labeled antibody binding to test line T205, and a lighter color at test line T205. Conversely, when the jujube seed saponin A content in the sample solution is low, fewer gold-labeled antibodies bind, resulting in more gold-labeled antibody binding to test line T205, and a darker color at test line T205. The goat anti-mouse antigen on control line C206 can bind to all gold-labeled antibodies. The content level of jujube seed saponin A is determined by directly comparing the colors of control line C206 and test line T205. A darker test line T205 indicates a lower concentration of the target analyte in the sample solution. The quantitative detection card can be read numerically by measuring the colors of test line T205 and control line C206 using a reader; different values ​​represent different contents.

[0028] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0029] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A test card for detecting the content of jujube seed saponin A in traditional Chinese medicinal materials, characterized in that, Includes an upper casing (1) and a lower casing (2). A test strip fixing casing (201) is installed at the bottom of the lower casing (2). A sample pad (202) is abutted inside the test strip fixing casing (201). A gold-labeled pad (203) is fixedly connected to the side of the sample pad (202). An NC membrane (204) is fixedly connected to the side of the gold-labeled pad (203). The NC membrane (204) has a detection line T (205) coated with jujube seed saponin A antigen and a control line C (206) coated with goat anti-mouse secondary antibody in its middle section. 04) has an absorption pad (207) fixedly connected to its side. The upper casing (1) and lower casing (2) are respectively fixedly connected to the sides of a fixing plate (303). The fixing plate (303) has two fixing sleeves (302) fixedly installed on its side. The two fixing sleeves (302) are each provided with a rubber elastic bead (305). The top of the rubber elastic bead (305) is attached to a pressing column (301). The top of the pressing column (301) is fixedly connected to a locking protrusion (304). The outer wall of the locking protrusion (304) is attached to a C-shaped locking block (3).

2. The detection card for detecting the content of jujube seed saponin A in traditional Chinese medicinal materials according to claim 1, characterized in that: The diameter of the cavity of the fixed sleeve (302) is greater than the diameter of the rubber elastic bead (305), and the inner wall of the fixed sleeve (302) is in contact with the outer wall of the pressing column (301).

3. The detection card for detecting the content of jujube seed saponin A in traditional Chinese medicinal materials according to claim 1, characterized in that: The upper casing (1) has a sample drop hole (102), and a conical drain shell (103) is fixedly connected inside the sample drop hole (102). A drain pipe (104) is fixedly installed at the bottom of the conical drain shell (103), and the bottom end of the drain pipe (104) is in contact with the top of the sample pad (202).

4. The detection card for detecting the content of jujube seed saponin A in traditional Chinese medicinal materials according to claim 1, characterized in that: The upper casing (1) has an observation window (101) located at the center.

5. The detection card for detecting the content of jujube seed saponin A in traditional Chinese medicinal materials according to claim 1, characterized in that: The lower housing (2) is equipped with a test paper fixing housing two (208), and the inner side of the test paper fixing housing two (208) abuts against the absorbent pad (207).

6. The detection card for detecting the content of jujube seed saponin A in traditional Chinese medicinal materials according to claim 1, characterized in that: The upper casing (1) and the lower casing (2) cooperate to form a hollow cuboid. The length and width of the upper casing (1) and the lower casing (2) are the same. The gold pad (203) is sprayed with colloidal gold particles adsorbed with anti-jujube seed saponin A monoclonal antibody or latex microspheres chemically bonded with anti-jujube seed saponin A monoclonal antibody.

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