A device for continuous labelling of indoor plants with carbon isotopes

Abstract

The utility model relates to a kind of indoor plant carbon isotope continuous marking device, belong to isotope tracer device technical field.The device includes marking box, gas mixing temperature and humidity device, reaction device, light supplementing device and connecting pipe.Mark box is made of box and base, gas mixing temperature and humidity device includes ordinary fan, air conditioner fan, dehumidification bag and ice box, reaction device includes control type infusion set, magnetic stirrer and reaction bottle, for continuously and stably generating 13 CO2, light supplementing device is divided into two parts plant light supplementing lamp in marking box, monitoring connecting pipe penetrates marking box body and is connected with high-precision carbon isotope analyzer, realizes the real-time monitoring of CO2 concentration and 13 C abundance.The utility model effectively overcomes the adverse effect of outdoor environment fluctuation on marking process, realizes continuous, stable, controllable carbon isotope marking, significantly improves experimental precision and efficiency, provides strong technical support for accurately revealing plant photosynthetic carbon allocation and turnover rule.

Description

Technical Field

[0001] This utility model belongs to the technical field of isotope tracing devices, and particularly relates to an indoor plant carbon isotope continuous labeling device. Background Technology

[0002] Isotope tracing technology uses radioactive or stable isotope atoms as "labels" to track the movement, transfer, and changes of matter. Because isotopes of the same element have the same chemical properties but different masses, isotopes undergo fractionation in physical, chemical, and biological processes, leading to systematic differences in the isotopic composition of reactants and products. By detecting these mass differences, the migration and transformation pathways of matter can be accurately tracked. Therefore, isotope tracing technology is widely used in research fields such as plant physiological ecology, soil science, and global change ecology, and is a key tool for studying the distribution, turnover, and transformation of photosynthetic carbon in the "plant-soil" system. This technology requires a system that isolates the plant from the external environment and allows for the injection of labeled carbon dioxide (such as...). 13 A closed labeling device (CO2) is used to create a stable and controllable labeling environment.

[0003] A plant carbon isotope labeling device is a closed artificial climate system used to provide plants with stable isotopes to track photosynthetic carbon allocation. Its core is a well-lit, sealed labeling chamber used to isolate the plant from the external environment. The device is equipped with lighting, temperature and humidity control, and gas circulation systems to create stable conditions for plant growth. The labeling gas is typically generated by reacting a carbon isotope source with an acid solution in the reaction apparatus and then delivered into the chamber for the plant to assimilate and absorb.

[0004] However, current plant carbon isotope labeling devices have certain limitations: 1. The labeling box uses air conditioning and humidifiers to regulate temperature and humidity. The airflow generated by these devices disrupts the indoor air distribution, leading to... 13 Uneven CO2 concentrations affect the consistency of plant markers; improper humidification control can also cause condensation, reducing light transmittance and interfering with photosynthesis.

[0005] 2. The supplemental light in the marking box is placed inside. The heat generated by the light accumulates in the sealed space, which raises the temperature inside the box. This increases the temperature control load and inhibits the activity of photosynthetic enzymes, reducing CO2 assimilation efficiency.

[0006] 3. The marking box did not match the contents of the device. 13 Real-time online monitoring of C abundance cannot achieve dynamic quantification and feedback control of the labeling effect.

[0007] In summary, existing carbon isotope labeling devices still have significant shortcomings in terms of the precision and non-interference nature of environmental control, the synergy between illumination and temperature control, and the monitorability of the labeling process. Therefore, there is an urgent need for a new type of labeling device that can overcome these shortcomings, achieve precise and stable environmental control, continuous and uniform labeling, and provide real-time feedback. Summary of the Invention

[0008] This invention provides an indoor plant carbon isotope continuous labeling device to solve the problems of existing devices in terms of the accuracy and non-interference of environmental control, the coordination of light and temperature control, and the monitorability of the labeling process.

[0009] The technical solution adopted by this utility model includes a marking box, a gas mixing temperature and humidity control device, a reaction device, a supplementary lighting device, and a monitoring connection tube, wherein: The marking box consists of a box body and a base, with the box body placed inside an annular water trough on the base; The mixed-air temperature and humidity control device includes a regular fan, an air conditioning fan, a dehumidifying bag, and an ice box. The regular fan is located on the top and side walls of the cabinet, the air conditioning fan is placed on the base, the dehumidifying bag is placed on the surface of the plant pots inside the cabinet, and the ice box is placed on the side walls and base of the cabinet. The reaction apparatus includes a controllable infusion set, a magnetic stirrer, and a reaction flask. The controllable infusion set is inserted through an opening at the top of the housing, with its end aligned with the reaction flask on the magnetic stirrer. Both the reaction flask and the magnetic stirrer are placed on a base. The supplemental lighting device includes external plant supplemental lights and internal plant supplemental lights. The external plant supplemental lights are installed below the top of the light frame, and the internal plant supplemental lights are located at the top of the box. The monitoring connection pipe passes through the side wall of the enclosure, with one end inside the enclosure and the other end outside. It is used to connect to a high-precision carbon isotope analyzer and is equipped with a manual control valve.

[0010] The box described in this utility model is a five-sided, bottomless cube made of acrylic glass.

[0011] The base described in this utility model is made of polyethylene.

[0012] The ordinary fan described in this utility model is located at the top four corners and above the inner side wall of the housing.

[0013] The air conditioning fan of this utility model is arranged in a triangular pattern.

[0014] The dehumidifier bag described in this invention contains orange color-changing silicone.

[0015] The upper end of the adjustable infusion set of this utility model is inserted into the drip bottle, and the drip bottle is hung on the infusion stand.

[0016] The lamp holder of this utility model is fixedly connected to a base support at the bottom, and a caster wheel is installed under the base support.

[0017] A temperature sensor is installed on the upper part of the inside of the box described in this utility model.

[0018] The outer wall of the box described in this utility model is equipped with a pair of handles.

[0019] The advantages of this utility model are: 1. This utility model achieves precise and stable control of temperature, humidity and light inside the chamber through a mixed-air temperature and humidity control device and an internal and external zoned supplemental lighting device, effectively avoiding problems such as internal airflow disturbance, temperature accumulation and condensation, and creating a uniform and stable climatic environment for plant growth and marking.

[0020] 2. The reaction rate is precisely controlled by a controllable infusion set, thereby continuously and stably supplying ¹³CO2 to the labeling chamber, avoiding the abundance fluctuations of traditional pulse labeling methods, and truly reflecting the continuous distribution and transformation dynamics of photosynthetic carbon.

[0021] 3. It is connected to a high-precision carbon isotope analyzer via a monitoring connection tube and is equipped with a manual control valve, which can monitor the CO2 concentration and ¹³C isotope abundance in the label box in real time and online as needed, greatly improving the accuracy, controllability and efficiency of the experiment.

[0022] Therefore, this utility model has a novel structure and integrates three core advantages: precise environmental control, continuous and stable labeling process, and real-time abundance monitoring. It effectively solves the key technical bottlenecks caused by external environmental fluctuations, internal temperature and light interference, discontinuous labeling process, and inability to quantify in real time in traditional devices. It has become an efficient, reliable, and controllable research tool, providing strong technical support for accurately revealing the distribution and turnover patterns of photosynthetic carbon in plants. Attached Figure Description

[0023] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2 This is a structural diagram of the box body, ordinary fan, ice box, partially adjustable infusion set, plant grow light inside the box, temperature sensor and handle of this utility model. Figure 3 This is a structural schematic diagram of the base, water tank, air conditioning fan, dehumidifier bag, ice box, magnetic stirrer and reaction flask of this utility model; Figure 4 This is a structural schematic diagram of the lamp holder of this utility model. Detailed Implementation

[0024] The present invention will now be further described in conjunction with specific embodiments. It should be noted that the accompanying drawings are merely illustrative and are not drawn to scale. Some conventional structures or components known to those skilled in the art may be omitted, and should not be construed as limiting the scope of protection of the present invention.

[0025] See Figures 1-4 It includes a marking box, a gas mixing and temperature / humidity control device, a reaction device, a supplementary lighting device, and a monitoring connection pipe, wherein: The marking box consists of a box body 1 and a base 2, with the box body 1 placed inside the annular water tank 3 of the base 2; The mixed-air temperature and humidity control device includes a regular fan 4, an air conditioning fan 5, a dehumidifying bag 6, and an ice box 7. The regular fan 4 is located on the top and side walls of the chamber 1 to achieve three-dimensional air circulation inside the chamber. The air conditioning fan 5 is placed on the base 2 to enhance airflow and assist in cooling. The dehumidifying bag 6 is placed on the surface of the plant pot 23 inside the chamber 1 for humidity control. The ice box 7 is located on the side walls of the chamber 1 and the base 2 for temperature control.

[0026] The reaction apparatus includes a controllable infusion set 8, a magnetic stirrer 9, and a reaction bottle 10. The controllable infusion set 8 is inserted through an opening at the top of the housing 1, with its end aligned with the reaction bottle 10 on the magnetic stirrer 9. Both the reaction bottle 10 and the magnetic stirrer 9 are placed on the base 2.

[0027] The supplemental lighting device includes an external plant grow light 16 and an internal plant grow light 11, providing the necessary energy source for plant photosynthesis. The external plant grow light 16 is installed below the top of the light stand 12, providing most of the light required for plant growth; the internal plant grow light 11 is located at the top of the box to compensate for the amount of light lost through the acrylic glass plate.

[0028] The monitoring connection pipe 13 passes through the side wall of the chamber 1, with one end inside the chamber and the other end outside, for connecting to the high-precision carbon isotope analyzer 21. The monitoring connection pipe 13 is equipped with a manual control valve 15 to control the flow of gas samples to the high-precision carbon isotope analyzer 21, thereby monitoring the CO2 concentration inside the chamber as needed. 13 C abundance.

[0029] The box 1 described in this utility model is a five-sided bottomless cube made of acrylic glass plate (light transmittance > 95%). The base 2 described in this utility model is made of polyethylene.

[0030] The ordinary fan 4 described in this utility model is located at the top four corners and above the inner side wall of the box.

[0031] The air conditioning fan 5 described in this utility model is arranged in a triangular pattern.

[0032] The dehumidifying bag 6 described in this invention contains orange color-changing silicone.

[0033] The upper end of the adjustable infusion set 10 of this utility model is inserted into the drip bottle 17 to adjust the infusion rate. The drip bottle 17 is hung on the infusion stand 22.

[0034] See Figure 4 The lamp holder 12 of this utility model is fixedly connected to the base support 14 below, and the universal wheel 18 is installed below the base support 14.

[0035] A temperature sensor 19 is installed on the upper part of the inside of the box 1 described in this utility model.

[0036] The outer wall of the box 1 of this utility model is equipped with a pair of handles 20.

[0037] The working principle of this utility model will be explained in detail below using a research case on the application of this utility model to the formation of soil organic matter from the fine root litter of Leymus chinensis.

[0038] (1) Based on the characteristics of plant growth and development and the arrangement of potted plants, calculate and customize the appropriate size of the marking box in advance. The size of the marking box customized in this case is as follows: Box 1 is 1.62m long × 1.08m wide × 0.6m high, water trough is 5cm high and 1.5cm wide, and light stand 12 is 1.63m long × 1.18m wide × 0.8~1.2m high.

[0039] (2) One week before the start of the experiment, the wild sheepgrass seedlings were transplanted into pot 23 and placed in base 2 according to the pre-designed spatial layout. Then, the sheepgrass was cultured in a controlled environment in a laboratory.

[0040] (3) Before the experiment begins, the components are checked and confirmed. The internal and external supplementary lights and ordinary fans are powered by external power sources, the magnetic stirrer is powered by batteries, and the air conditioning fan is rechargeable. The connection of each device is completed in sequence, and the box is installed in the water tank 3. Water is added to the water tank. Finally, a comprehensive airtightness test is performed on the entire carbon isotope labeling device to ensure that it meets the experimental requirements. (4) One hour before the start of the experiment, open chamber 1, isolate the soil in the potted plants with a sealed bag, and place the dehumidifying bag 6 on the surface of the sealed bag; place the ice box 7 on the inner wall of the chamber and in the gaps between the potted plants; slowly pour the excess dilute sulfuric acid into reaction flask 10 (500mL glass beaker); add the weighed Na2 13CO3 is dissolved in deionized water and poured into drip bottle 17 (500mL glass bottle), which is then suspended on infusion stand 22. The drip bottle is connected to the adjustable infusion set 8. The power is turned on, and the magnetic stirrer 9, ordinary fan 4, air conditioning fan 5, and high-precision carbon isotope analyzer 21 are turned on. After everything is ready, two people work together to slowly place the box into the U-shaped water tank on the base, fill it with water to seal it, forming a sealed carbon isotope marking box. The light stand 12 is pushed directly above the marking box, and all the plant grow lights inside and outside the box are turned on to allow the sheepgrass to assimilate the contents of the box. 12 To improve the temperature control and plant assimilation effects within the CO2 chamber, the device can be placed in an air-conditioned room.

[0041] (5) After the label box is closed for 30 minutes, turn on the controllable infusion set and continuously add Na2 to the reaction bottle containing dilute sulfuric acid at the preset flow rate for an extended period of time. 13 CO3 solution, reaction produces 13 CO2 is continuously supplied to the sheepgrass for assimilation and absorption. During this process, the manual control valve 15 on the monitoring connection pipe 13 can be opened every 30 minutes as needed to monitor the CO2 concentration inside the chamber. 13 For C abundance, after each monitoring of the gas level inside the chamber, the valve should be closed to prevent gas waste and leakage.

[0042] (6) After one day of carbon isotope labeling is completed, first turn off the magnetic stirrer, fan, air conditioning fan, and high-precision carbon isotope analyzer. Remove the lamp stand and infusion set. Two people work together to lift the chamber out and remove the dehumidifying bag, ice box, air conditioning fan, reaction flask, and magnetic stirrer. This completes one day of carbon isotope labeling. According to the experimental requirements, repeat the above operation every day for long-term carbon isotope labeling.

[0043] (7) After continuous carbon isotope labeling is completed, collect all the fine roots of Leymus chinensis in the pot, wash, dry and mix them, take a small amount of dry matter and use an isotope mass spectrometer to determine the concentration of carbon in the fine root litter. 13 C abundance.

[0044] This invention utilizes a polyethylene base to effectively prevent water and soil from seeping out of the pot during indoor cultivation of Leymus chinensis, thus preventing contamination of the laboratory environment. By placing it in an air-conditioned room, and through the combined action of an air conditioning fan, a regular fan, and an ice pack, a low temperature can be regulated and maintained inside the labeling chamber, effectively avoiding the high-temperature environment caused by prolonged closure of the labeling chamber and ensuring the high efficiency and accuracy of isotope labeling. The arrangement of dehumidifying bags continuously absorbs excess moisture from the sealed system, preventing excessive humidity from condensing and affecting light transmittance. Precise control via a regulating infusion set enables the precise labeling of Na2... 13 The CO3 solution is added dropwise at a constant rate to ensure a continuous and stable release of CO3 from the sulfuric acid. 13CO2 provides a stable carbon source for plant photosynthesis. Plant supplemental lighting, strategically placed inside and outside the labeling chamber, not only provides ample and uniform illumination to meet the photosynthetic needs of *Leymus chinensis*, but its adjustable light intensity and photoperiod characteristics also simulate natural light environments or create specific experimental conditions, ensuring normal plant growth during the labeling period. A manual control valve on the monitoring tube connects to a high-precision carbon isotope analyzer, allowing for on-demand, real-time monitoring and feedback of the CO2 concentration within the labeling chamber. 13 The dynamic changes in C abundance provide crucial data support for accurately assessing labeling efficiency and adjusting experimental parameters.

[0045] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A continuous carbon isotope labeling device for indoor plants, characterized in that: Includes a marking box, a gas mixing and temperature / humidity control device, a reaction device, a supplementary lighting device, and a monitoring connection pipe, wherein: The marking box consists of a box body and a base, with the box body placed inside an annular water trough on the base; The mixed-air temperature and humidity control device includes a regular fan, an air conditioning fan, a dehumidifying bag, and an ice box. The regular fan is located on the top and side walls of the cabinet, the air conditioning fan is placed on the base, the dehumidifying bag is placed on the surface of the plant pots inside the cabinet, and the ice box is placed on the side walls and base of the cabinet. The reaction apparatus includes a controllable infusion set, a magnetic stirrer, and a reaction flask. The controllable infusion set is inserted through an opening at the top of the housing, with its end aligned with the reaction flask on the magnetic stirrer. Both the reaction flask and the magnetic stirrer are placed on a base. The supplemental lighting device includes external plant supplemental lights and internal plant supplemental lights. The external plant supplemental lights are installed below the top of the light frame, and the internal plant supplemental lights are located at the top of the box. The monitoring connection pipe passes through the side wall of the enclosure, with one end inside the enclosure and the other end outside. It is used to connect to a high-precision carbon isotope analyzer and is equipped with a manual control valve.

2. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: The box is a five-sided, bottomless cube made of acrylic glass.

3. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: The base is made of polyethylene.

4. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: The ordinary fan is located at the top four corners and above the inner side wall of the box.

5. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: The air conditioning fans are arranged in a triangular pattern.

6. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: The dehumidifier bag contains orange color-changing silicone.

7. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: The upper end of the adjustable infusion set is inserted into the drip bottle, which is then hung on the infusion stand.

8. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: The base support is fixedly connected to the bottom of the lamp holder, and casters are installed under the base support.

9. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: A temperature sensor is installed at the top inside the enclosure.

10. The indoor plant carbon isotope continuous labeling device according to claim 1, characterized in that: Each of the outer walls of the box is equipped with a pair of handles.

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