A cell culture dish

Abstract

The utility model discloses a cell culture dish relates to cell culture technical field, and the utility model discloses a dish body is personally experienced sth, the inside of dish body is equipped with the separation component, the upper surface of dish body, the gas permeable component includes the gas permeable hole, the gas permeable hole is set up in the upper surface of sealing cover, and the inner wall of gas permeable hole is fixedly connected with first round plate, and the application realizes the flexible adjustment of gas permeable hole size through the gas permeable component, can according to cell type, the actual demand such as culture period, the accurate control gas exchange rate, simultaneously, the filter screen built -in of this component can high -efficiently intercept the outside microorganism, particulate impurity to make the culture dish realize the ideal gas permeable effect, and the application is through the separation component, and the interior space of its culture dish is reasonably flexible and is separated, can be accurate control the spatial distribution of different cells in the co -culture experiment, and the use efficiency of culture space is optimized, and the functional potential of culture dish is exerted.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a cell culture dish. Background Technology

[0002] A petri dish is a laboratory vessel used for the culture of microorganisms or cells. It consists of a flat, disc-shaped base and a lid, and is generally made of glass or plastic. Petri dishes are basically divided into two categories: plastic and glass. Glass dishes can be used for the culture of plant materials, microorganisms, and may also be used for the adherent culture of animal cells. Plastic dishes are likely made of polyethylene and are available in disposable and reusable versions. They are suitable for laboratory inoculation, streaking, and bacterial isolation operations, and can be used for the culture of plant materials. However, existing cell culture dishes still have some problems when used: First, the existing aeration structures in cell culture dishes are too simple, generally consisting of simple vents and sealing plugs. During the culture process, the size of the vents cannot be flexibly adjusted according to the actual culture requirements, and there is a lack of gas filtration structures, thus failing to achieve the best aeration effect during culture. Secondly, the internal space of existing cell culture dishes is designed as a whole, which means that different cells can only be directly mixed in co-culture experiments, and the spatial distribution cannot be controlled, resulting in low utilization efficiency of the culture space. Utility Model Content

[0003] To address the issues of limited design of the breathable structure and lack of scientific functional zoning in the internal space of existing cell culture dishes, the purpose of this invention is to provide a cell culture dish.

[0004] To solve the above-mentioned technical problems, the present invention adopts the following technical solution: a cell culture dish, including a dish body, wherein a partition component is provided inside the dish body, a sealing cap is movably snapped onto the upper surface of the dish body, and multiple sets of venting components are provided on the upper surface of the sealing cap. Each venting component includes a vent hole, which is opened on the upper surface of the sealing cap. A first circular plate is fixedly connected to the inner wall of the vent hole. A first circular hole is arranged in a ring array on the upper surface of the first circular plate. A second circular plate is rotatably connected to the upper surface of the first circular plate through a rotating shaft damping, and a second circular hole is arranged in a ring array on the upper surface of the second circular plate to cooperate with the first circular hole. A rotating rod is fixedly connected to the upper surface of the second circular plate.

[0005] Preferably, the separating component includes a column, which is fixedly installed inside the dish body. The outer surface of the column has a first slot arranged in a ring array, and the inside of the dish body has a second slot arranged in a ring array. The separating plate is movably engaged with the corresponding first and second slots.

[0006] Compared with the prior art, the beneficial effects of this utility model are as follows: 1. This application achieves flexible adjustment of the size of the vent holes through the venting component, which can precisely control the gas exchange rate according to actual needs such as cell type and culture cycle. At the same time, the filter built into the component can efficiently intercept external microorganisms and particulate impurities, thereby enabling the culture dish to achieve ideal venting effect. 2. This application uses a partition component to rationally and flexibly divide the internal space of the culture dish, which can precisely control the spatial distribution of different cells in co-culture experiments, optimize the utilization efficiency of the culture space, and give full play to the functional potential of the culture dish. Attached Figure Description

[0007] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0008] Figure 1 This is a schematic diagram of the structure of this utility model.

[0009] Figure 2 This is a schematic diagram of the explosive structure of this utility model.

[0010] Figure 3 This is a schematic diagram of the exploded cross-sectional structure of the breathable component of this utility model.

[0011] Figure 4 This utility model Figure 3 Enlarged schematic diagram of the structure at point A in the middle.

[0012] Figure 5 This is a schematic diagram of the exploded structure of the separator component of this utility model.

[0013] In the diagram: 1. Dish body; 2. Ventilation component; 21. Ventilation hole; 22. Rotary rod; 23. Second circular plate; 24. First circular plate; 25. Filter screen; 26. Mounting groove; 27. First circular hole; 28. Second circular hole; 3. Separator component; 31. Positioning sleeve; 32. Separator plate; 33. Second slot; 34. First slot; 35. Column; 4. Sealing cap; 5. First magnetic block; 6. Second magnetic block. Detailed Implementation

[0014] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0015] Example: Figure 1-5 As shown, this utility model provides a cell culture dish, including a dish body 1, which serves as the basic carrier for cell culture and provides a space for containing cells. The interior of the dish body 1 is provided with a partition component 3, which can divide the interior space of the dish body 1. A sealing cap 4 is movably snapped onto the upper surface of the dish body 1. A first magnetic block 5 is symmetrically fixedly installed on the outer surface of the dish body 1, and a second magnetic block 6 is symmetrically fixedly installed on the outer surface of the sealing cap 4. The first magnetic block 5 and the second magnetic block 6 are magnetically snapped together. Compared with traditional snap-fit ​​or threaded connections, the magnetic snap-fit ​​method of the sealing cap 4 with the first magnetic block 5 and the second magnetic block 6 is more convenient to operate and can quickly realize the installation and removal of the sealing cap 4 and the dish body 1. At the same time, the magnetic connection is tight and can effectively prevent external contamination from entering the culture environment. The upper surface of the sealing cap 4 is provided with multiple sets of venting components 2. The multiple sets of venting components 2 work together to achieve ideal venting effect in the culture dish.

[0016] The ventilated component 2 includes a ventilated hole 21, which is located on the upper surface of the sealing cover 4. The ventilated hole 21 serves as a channel for gas to enter and exit. A first circular plate 24 is fixedly connected to the inner wall of the ventilated hole 21. A first circular hole 27 is arranged in a ring on the upper surface of the first circular plate 24. A second circular plate 23 is rotatably connected to the upper surface of the first circular plate 24 via a rotating shaft. The outer surface of the second circular plate 23 is rotatably connected to the inner wall of the ventilated hole 21. A second circular hole 28, which is used in conjunction with the first circular hole 27, is arranged in a ring on the upper surface of the second circular plate 23. An installation groove 26 is provided inside the ventilated hole 21. A filter screen 25, which is used in conjunction with the first circular hole 27 and the second circular hole 28, is fixedly installed inside the installation groove 26. The filter screen 25, installed in the installation groove 26, can effectively intercept external microorganisms and particulate impurities, preventing them from entering the culture environment through the ventilated hole 21.

[0017] A rotating rod 22 is fixedly connected to the upper surface of the second circular plate 23. The first circular plate 24 and the second circular plate 23 cooperate with the first circular hole 27 and the second circular hole 28. By rotating the rotating rod 22, the second circular plate 23 can be rotated, which can flexibly adjust the overlapping area of ​​the first circular hole 27 and the second circular hole 28, thereby changing the ventilation volume of the vent 21. The ventilation effect can be precisely adjusted according to the gas exchange requirements of different cell types and culture stages.

[0018] The partition component 3 includes a column 35, which is fixedly installed inside the dish 1. The outer surface of the column 35 has a first slot 34 arranged in a ring array, and the inside of the dish 1 has a second slot 33 arranged in a ring array. The partition plate 32 is movably engaged with the corresponding first slot 34 and second slot 33. The first slot 34 and second slot 33 cooperate to fix the partition plate 32. By changing the installation position and number of partition plates 32, the internal space of the dish 1 can be flexibly partitioned to realize the partition culture of multiple cells, accurately control the spatial distribution of different cells, and facilitate the study of cell interactions.

[0019] The upper surface of the column 35 is movably engaged with a positioning sleeve 31 for use with the partition plate 32. The sealing cover 4 is movably engaged with the upper surface of the positioning sleeve 31. The positioning sleeve 31 is engaged with the column 35, which can position the top of the partition plate 32 and enhance the stability of the partition plate 32 after installation. The movable engagement of the sealing cover 4 with the upper surface of the positioning sleeve 31 further enhances the stability of the partition plate 32 engagement. The partition plate 32 is a sealing plate, which effectively improves the sealing performance of the overall structure, making each partition area relatively independent and not interfering with each other, thus improving the rationality and scientificity of the internal space utilization of the petri dish.

[0020] Working principle: First, according to the experimental requirements, the internal space of the dish 1 is divided using the partition component 3. The partition plate 32 is used as a sealing plate and is snapped into the first slot 34 on the outer surface of the column 35 and the second slot 33 inside the dish 1. By flexibly adjusting the number and position of the partition plates 32, the interior of the dish 1 can be divided into multiple independent culture areas, thereby realizing the partition culture of different cells and facilitating precise control of the spatial distribution between cells.

[0021] After the space is divided, the positioning sleeve 31 is snapped onto the upper surface of the column 35 to position the partition plate 32 from the top, further enhancing the stability of the partition plate 32 and preventing it from shaking or shifting in subsequent operations.

[0022] Next, the cells to be cultured are placed in the appropriate positions inside the dish 1. Then, the magnetic attraction between the first magnetic block 5 on the outer surface of the dish 1 and the second magnetic block 6 on the outer surface of the sealing cap 4 is used to securely cover the dish 1, effectively preventing the intrusion of external microorganisms, dust and other impurities, ensuring the sterility of the culture environment, and enabling quick opening of the cap for sampling or observation during the experiment, significantly improving the convenience of operation.

[0023] During cell culture, the ventilated component 2 plays a crucial role. The vent 21 serves as a channel for gas to enter and exit. By rotating the rotating rod 22, the experimenter can drive the second circular plate 23, which is connected to the first circular plate 24 for damping rotation, to rotate, thereby changing the overlapping area of ​​the first circular hole 27 and the second circular hole 28, and thus flexibly adjusting the ventilation volume of the vent 21 to meet the gas exchange needs of different cells.

[0024] Meanwhile, the filter 25 fixed in the mounting groove 26 inside the vent 21 can effectively intercept external microorganisms and particulate impurities, ensuring gas flow while maintaining the cleanliness of the culture environment and ensuring ideal air permeability during cell culture.

[0025] Throughout the cell culture process, the separator 3 scientifically divides the culture area as needed, the ventilator 2 precisely adjusts gas exchange according to the actual needs of the cells, and the sealing cap 4 is tightly connected to the dish body 1 by magnetic attraction. The three work together to provide a stable and controllable culture environment for the cells, fully meeting the diverse needs of cell culture experiments.

[0026] Obviously, those skilled in the art can make various modifications and variations to this utility model without departing from its spirit and scope. Therefore, if these modifications and variations fall within the scope of the claims of this utility model and their equivalents, this utility model also intends to include these modifications and variations.

Claims

1. A cell culture dish, comprising a dish body (1), characterized in that: The interior of the dish body (1) is provided with a partition component (3), and a sealing cap (4) is movably attached to the upper surface of the dish body (1). The upper surface of the sealing cap (4) is provided with multiple sets of breathable components (2). The breathable component (2) includes a breathable hole (21), which is opened on the upper surface of the sealing cover (4). The inner wall of the breathable hole (21) is fixedly connected to a first circular plate (24). The upper surface of the first circular plate (24) is provided with a first circular hole (27) in an annular array. The upper surface of the first circular plate (24) is rotatably connected to a second circular plate (23) through a rotating shaft damping. The upper surface of the second circular plate (23) is provided with a second circular hole (28) in an annular array to cooperate with the first circular hole (27). The upper surface of the second circular plate (23) is fixedly connected to a rotating rod (22).

2. The cell culture dish as described in claim 1, characterized in that: The partition component (3) includes a column (35), which is fixedly installed inside the dish body (1). The outer surface of the column (35) is provided with a first slot (34) in an annular array, and the inside of the dish body (1) is provided with a second slot (33) in an annular array. The partition plate (32) is movably engaged inside the corresponding first slot (34) and second slot (33).

3. The cell culture dish as described in claim 1, characterized in that: The outer surface of the dish body (1) is symmetrically fixed with a first magnetic block (5), and the outer surface of the sealing cover (4) is symmetrically fixed with a second magnetic block (6). Correspondingly, the first magnetic block (5) and the second magnetic block (6) are magnetically attracted to each other.

4. The cell culture dish as described in claim 1, characterized in that: The ventilation hole (21) has an installation groove (26) inside, and a filter screen (25) that is used in conjunction with the first round hole (27) and the second round hole (28) is fixedly installed inside the installation groove (26).

5. A cell culture dish as described in claim 1, characterized in that: The outer surface of the second circular plate (23) is damped and rotatedly connected to the inner wall of the vent hole (21).

6. A cell culture dish as described in claim 2, characterized in that: The partition plate (32) is a sealing plate.

7. A cell culture dish as described in claim 2, characterized in that: The upper surface of the column (35) is movably engaged with a positioning sleeve (31) for use with the partition plate (32), and the sealing cover (4) is movably engaged with the upper surface of the positioning sleeve (31).

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