An efficient ultra-micro pathological sample preparation device

Abstract

The utility model discloses a kind of high-efficiency ultra-micro pathological sample preparation devices, belong to biological tissue section technical field, the embedding block placement seat of multiple different through slot number, through slot interval and corresponding different blade number, blade interval multi-blade slice seat of the present application can be selected to replace different thickness demand and different slice quantity demand, and the embedding block placement seat of the present application is the structure of one end opening, plus transverse propulsion mechanism extrudes embedding block from embedding block placement seat opening direction, so that embedding block of different size, shape can be fixed on embedding block placement seat, in conclusion, the present application realizes the technical effect of high-efficiency section, and can adapt to embedding block of multiple different size, shape, improve the universality of the present application.

Description

Technical Field

[0001] This invention belongs to the field of biological tissue sectioning technology, specifically relating to a highly efficient ultramicro pathological sample preparation device. Background Technology

[0002] The general procedure for preparing pathological samples includes: removing tissue from a living organism, cutting it into small pieces of about 1 mm³, fixing the pieces before and after, dehydrating them, embedding and polymerizing them to form hard embedded blocks, sectioning the embedded blocks, and finally staining the sections to obtain pathological samples.

[0003] However, during typical slicing operations, the slicing machine slices the embedded blocks one slice at a time, which is inefficient. Furthermore, the slicing machine has requirements for the shape and size of the embedded blocks, making it impossible to adapt to various types of embedded blocks and increasing the workload of the operators. Utility Model Content

[0004] To address the problems mentioned in the background art, this utility model provides a highly efficient ultra-micro pathological sample preparation device to solve the problem that the efficiency of existing slicers needs to be improved and that they cannot adapt to various types of embedding blocks during the slice operation.

[0005] To achieve the above objectives, this utility model provides the following technical solution: A highly efficient ultramicro pathological sample preparation device, comprising: Mounting substrate; The mounting substrate is provided with a rectangular placement slot; An embedding block placement seat; the embedding block placement seat is a cubic box structure, which consists of a base plate, a first side wall, a second side wall, and a third side wall. The embedding block placement seat does not have a top surface or a fourth side wall. The two parallel side walls are the first side wall and the third side wall, respectively. The embedding block placement seat has multiple parallel through slots. The first end of one through slot is located on the top surface of the first side wall, and the other end passes through the inner surface of the first side wall, the top surface of the base plate, and the inner surface of the third side wall in sequence before extending to the top surface of the third side wall. The base plate is placed in a rectangular placement slot. The top surface of the base plate is flush with the top surface of the mounting substrate. Each side of the base plate is in contact with the inner surface of the rectangular placement slot. The embedding block is placed in the embedding block placement seat, and the embedding block covers the portion of all through slots located on the base plate. Lateral propulsion mechanism; The lateral propulsion mechanism is mounted on the mounting base plate. The lateral propulsion mechanism includes a lateral power output end and a lateral movement end. The lateral power output end is used to drive the lateral movement end to laterally squeeze or release the compression of the embedding block set in the embedding block placement seat, so that the embedding block is squeezed into contact or released from contact with the inner side of the second side wall. Longitudinal propulsion mechanism; The longitudinal propulsion mechanism is mounted on the mounting base plate and includes a mounting frame, a longitudinal power output end and a longitudinal motion end. The longitudinal power output end is mounted on the mounting frame and the longitudinal motion end is positioned pointing towards the top surface of the base plate of the embedding block placement seat. Multi-blade slicing holder; The multi-blade slicing holder includes a base and a blade. The blade is composed of multiple blades arranged parallel to each other on the first surface of the base. The second surface of the base is detachably connected to the longitudinal moving end. The longitudinal pushing mechanism is used to drive the blades in the blade to be inserted into or withdrawn from the grooves one by one. When the blade moves to the lower extreme point, the two sides of the blade and the cutting edge are respectively located in the inner side of the first side wall, the inner side of the third side wall, and the top surface of the base plate. Control module; both the lateral power output end and the longitudinal power output end are connected to the control module, which is used to output preset control commands to the lateral power output end and the longitudinal power output end.

[0006] Preferably, the lateral propulsion mechanism includes: Lateral telescopic motor; The lateral telescopic motor is connected to the control module and is fixedly mounted on the mounting base plate. The telescopic rod of the lateral telescopic motor is set to point towards the inner side of the second side wall. Elastic buffer mechanism; the first end of the elastic buffer mechanism is connected to the telescopic rod of the lateral telescopic motor; Push plate; the push plate is connected to the second end of the elastic buffer mechanism, and the push plate and the second side wall are arranged parallel to each other.

[0007] Preferably, the buffer mechanism includes: Mounting plate; the first side of the mounting plate is fixedly connected to the end face of the telescopic rod of the horizontal telescopic motor, and the mounting plate is provided with at least one positioning through hole; At least one positioning rod; the first end of the positioning rod is fixedly installed on the first surface of the push plate, and the second end of the positioning rod passes through the first positioning through hole; Spring; one end of the spring is fixedly mounted on the second surface of the mounting plate, and the other end of the spring is fixedly mounted on the first surface of the push plate.

[0008] Preferably, a rubber pad is provided on the second surface of the push plate.

[0009] Preferably, the longitudinal propulsion mechanism includes: Two support plates; the two support plates are vertically and fixedly installed on the mounting base plate; Connecting plate; the connecting plate is positioned between the tops of the two support plates; Longitudinal telescopic motor; The longitudinal telescopic motor is connected to the control module. The longitudinal telescopic motor is fixedly installed on the top surface of the connecting plate, and the connecting plate is provided with a first through hole. The telescopic rod of the longitudinal telescopic motor passes through the first through hole and points to the top surface of the bottom plate of the embedded block placement seat.

[0010] Preferably, the high-efficiency ultramicro pathological sample preparation device also includes a rotating mechanism, which is used to drive the connecting plate to rotate.

[0011] Preferably, the rotating mechanism includes: Motor mount; The motor mount is fixedly installed on the mounting base plate; Drive motor; The drive motor is fixedly mounted on the motor mount; Two second through holes and a third through hole; one second through hole connects the top two sides of a support plate, and the third through hole is set through the two sides of the connecting plate; the central axes of the two second through holes and the third through hole are coaxial. Two bearings; one bearing is set in a second through hole, and the outer ring of the bearing is fixedly connected to the inner wall of the second through hole. The shaft of the drive motor passes through one bearing, a third through hole and another bearing in sequence, wherein the shaft of the drive motor is fixedly connected to the inner ring of each bearing and the shaft of the drive motor is fixedly connected to the third through hole.

[0012] Preferably, the blade is a diamond tool.

[0013] Compared with the prior art, the beneficial effects of this utility model are: This application allows for the selection and replacement of various embedding block placement seats with different numbers and spacings of through slots, as well as corresponding multi-blade slicing seats with different numbers and spacings of blades, to meet different thickness and slice quantity requirements. Furthermore, the embedding block placement seat of this application has an open-end structure, and the lateral propulsion mechanism compresses the embedding block from the opening direction of the embedding block placement seat, so that embedding blocks of different sizes and shapes can be fixed on the embedding block placement seat. In summary, this application achieves the technical effect of efficient slicing and can adapt to various sizes and shapes of embedding blocks, thus improving the versatility of this application. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the specific structure of this application; Figure 2 A schematic diagram showing the positional relationship between the embedding block placement seat and the push plate; Figure 3 A schematic diagram of the specific structure of the embedding block placement seat; The diagram is marked as follows: 1-Mounting base plate; 2-Motor base; 3-Drive motor; 4-Longitudinal telescopic motor; 5-Seat; 6-Blade; 7-Support plate; 8-Embedded block placement seat; 9-Rubber pad; 10-Push plate; 11-Spring; 12-Positioning rod; 13-Mounting plate; 14-Transverse telescopic motor. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Example 1 like Figure 1 , Figure 2 and Figure 3 As shown, a highly efficient ultramicro pathological sample preparation device includes: Mounting substrate 1; Mounting substrate 1 is provided with a rectangular placement slot; Embedded block placement seat 8; Embedded block placement seat 8 has a cubic box structure, which consists of a base plate, a first side wall, a second side wall and a third side wall. Embedded block placement seat 8 does not have a top surface and a fourth side wall. The two parallel side walls are the first side wall and the third side wall, respectively. Embedded block placement seat 8 has multiple parallel through slots. The first end of one through slot is located on the top surface of the first side wall, and the other end passes through the inner side surface of the first side wall, the top surface of the base plate, and the inner side surface of the third side wall in sequence before extending to the top surface of the third side wall. The base plate is placed in a rectangular placement slot. The top surface of the base plate is flush with the top surface of the mounting base plate 1. Each side of the base plate is in contact with the inner side surface of the rectangular placement slot. Embedded block placement seat 8 is fixed by friction. When replacing, it can be pulled out and inserted simply by overcoming friction. The embedded block is placed in embedded block placement seat 8, and the embedded block covers the part of all through slots located on the base plate. Lateral propulsion mechanism; The lateral propulsion mechanism is mounted on the mounting base plate 1. The lateral propulsion mechanism includes a lateral power output end and a lateral movement end. The lateral power output end is used to drive the lateral movement end to laterally squeeze or release the squeeze on the embedding block set in the embedding block placement seat 8, so that the embedding block is squeezed into contact or released from contact with the inner side of the second side wall. Longitudinal propulsion mechanism; The longitudinal propulsion mechanism is mounted on the mounting base plate 1. The longitudinal propulsion mechanism includes a mounting frame, a longitudinal power output end and a longitudinal motion end. The longitudinal power output end is mounted on the mounting frame, and the longitudinal motion end is positioned pointing towards the top surface of the bottom plate of the embedding block placement seat 8. Multi-blade slicing holder; The multi-blade slicing holder includes a base 5 and a blade. The blade is composed of multiple blades 6 arranged parallel to each other on the first surface of the base 5. The second surface of the base 5 is detachably connected to the longitudinal moving end. The longitudinal pushing mechanism is used to drive the blades 6 in the blade to be inserted into or withdrawn from the grooves one-to-one. When the blade moves to the lower extreme point, the two sides and the cutting edge of the blade 6 are respectively located in the inner side of the first side wall, the inner side of the third side wall, and the top surface of the base plate. Control module; Both the lateral and longitudinal power output ends are connected to the control module. The control module is used to output preset control commands to the lateral and longitudinal power output ends. The control module adopts the ACS MotionControl series module.

[0017] This application allows for the selection and replacement of various embedding block placement seats 8 with different numbers and spacings of through slots and corresponding multi-blade slicing seats with different numbers and spacings of blades 6 to meet different thickness and slice quantity requirements. For example, if a slice thickness in the mm range is required, a multi-blade slicing seat with a blade spacing in the mm range will be used. When a smaller slice thickness, such as μm or nm, is required, a blade spacing of corresponding intervals will be used. At the same time, an appropriate blade thickness will be selected according to the actual situation. Furthermore, the embedding block placement seat 8 of this application has an open structure at one end. With the addition of a transverse pushing mechanism that compresses the embedding block from the opening direction of the embedding block placement seat 8, embedding blocks of different sizes and shapes can be fixed on the embedding block placement seat 8. In summary, this application achieves the technical effect of efficient slicing and can adapt to various sizes and shapes of embedding blocks, thus improving the versatility of this application.

[0018] Example 2 The difference between this embodiment and Embodiment 1 is that, as Figure 1 , Figure 2 and Figure 3 As shown, the lateral propulsion mechanism includes: Lateral telescopic motor 14; The lateral telescopic motor 14 is connected to the control module and is fixedly mounted on the mounting base plate 1. The telescopic rod of the lateral telescopic motor 14 is set to point towards the inner side of the second side wall. Elastic buffer mechanism; the first end of the elastic buffer mechanism is connected to the telescopic rod of the transverse telescopic motor 14; Push plate 10; push plate 10 is connected to the second end of the elastic buffer mechanism, and push plate 10 and the second side wall are arranged parallel to each other.

[0019] Example 3 The difference between this embodiment and Embodiment 2 is that, as Figure 1 As shown, the buffer mechanism includes: Mounting plate 13; the first surface of mounting plate 13 is fixedly connected to the end face of the telescopic rod of the transverse telescopic motor 14, and two positioning through holes are provided on mounting plate 13; Two positioning rods 12; the first end of the positioning rod 12 is fixedly installed on the first surface of the push plate 10, and the second end of the positioning rod 12 passes through the first positioning through hole. Spring 11; one end of spring 11 is fixedly installed on the second surface of mounting plate 13, and the other end of spring 11 is fixedly installed on the first surface of push plate 10.

[0020] In this embodiment, the embedding block is rigid. If the advancing distance of the lateral telescopic motor 14 is adjusted according to the length of the embedding block each time, there may be problems such as insufficient advancing distance, loosening of the embedding block, and excessive advancing, which may cause the embedding block to be squeezed and cracked. Therefore, this application sets up a buffer mechanism, which can solve the above problems by storing energy through the spring 11 when the lateral telescopic motor 14 advances.

[0021] Example 4 The difference between this embodiment and embodiment 3 is that, as Figure 1 , Figure 2 As shown, a rubber pad 9 is provided on the second surface of the push plate 10, which can provide further buffer protection between the embedded block and the buffer mechanism.

[0022] Example 5 The difference between this embodiment and Embodiment 1 is that, as Figure 1 As shown, the longitudinal propulsion mechanism includes: Two support plates 7; the two support plates 7 are vertically fixed on the mounting base plate 1; Connecting plate; the connecting plate is disposed between the top ends of the two support plates 7; Longitudinal telescopic motor 4; The longitudinal telescopic motor 4 is connected to the control module. The longitudinal telescopic motor 4 is fixedly installed on the top surface of the connecting plate, and the connecting plate is provided with a first through hole. The telescopic rod of the longitudinal telescopic motor 4 passes through the first through hole and points to the top surface of the base plate of the embedding block placement seat 8. The end of the telescopic rod of the longitudinal telescopic motor 4 is provided with a thread. The second side of the seat 5 of the multi-blade slicing seat is provided with a screw hole. The two are connected by threads to achieve a detachable connection. In order to ensure that the relative position of the two is consistent each time they are tightened, a mark is provided on the thread and the screw hole. During installation, the two marks are aligned, and the lead, number of threads and direction of rotation of the thread and the screw hole are strictly matched. It is designed that when tightened to a fixed position, the cumulative rotation angle is an integer multiple of 360°. Thus, the alignment of the marks determines that the relative position of the two is consistent each time they are tightened.

[0023] Example 6 The difference between this embodiment and embodiment 5 is that, as Figure 1 As shown, the high-efficiency ultramicro pathological sample preparation device also includes a rotating mechanism, which is used to drive the connecting plate to rotate.

[0024] In this embodiment, when the multi-blade slicing holder is in the downward-facing working state, it is inconvenient to replace the multi-blade slicing holder or remove the slices between the blades 6. Therefore, this application provides a rotating mechanism that can rotate the drive connecting plate to change its orientation and increase the operating space of the multi-blade slicing holder.

[0025] Example 7 The difference between this embodiment and embodiment 6 is that, as Figure 1As shown, the rotating mechanism includes: Motor mount 2; Motor mount 2 is fixedly mounted on mounting base plate 1; Drive motor 3; Drive motor 3 is fixedly mounted on motor base 2, and drive motor 3 is connected to the control module; Two second through holes and a third through hole; one second through hole connects the top two sides of a support plate 7, and the third through hole is set through the two sides of the connecting plate. The central axes of the two second through holes and the third through hole are coaxial. Two bearings; one bearing is set in a second through hole, and the outer ring of the bearing is fixedly connected to the inner wall of the second through hole. The shaft of the drive motor 3 passes through one bearing, a third through hole and another bearing in sequence. The shaft of the drive motor 3 is fixedly connected to the inner ring of each bearing and to the third through hole.

[0026] Example 8 The difference between this embodiment and Embodiment 1 is that the blade 6 is a diamond cutter. Diamond cutters have extremely high hardness and very sharp cutting edges, enabling ultra-thin slicing with a thickness that can be controlled from tens of nanometers to a few micrometers. Furthermore, the cut of a diamond cutter is smooth and even, which can reduce sample damage.

[0027] Those skilled in the art will recognize that the embodiments described herein are intended to help the reader understand the principles of this application, and should be understood as not limiting the scope of protection of this application to such specific statements and embodiments. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. A highly efficient ultramicro pathological sample preparation device, characterized in that, include: Mounting substrate (1); The mounting substrate (1) is provided with a rectangular placement slot; Embedded block placement seat (8); The embedded block placement seat (8) is a cubic box structure, which consists of a bottom plate, a first side wall, a second side wall and a third side wall. The embedded block placement seat (8) does not have a top surface and a fourth side wall. The two parallel side walls are the first side wall and the third side wall, respectively. The embedded block placement seat (8) has multiple parallel through slots. The first end of one through slot is located on the top surface of the first side wall, and the other end passes through the inner side surface of the first side wall, the top surface of the bottom plate, and the inner side surface of the third side wall in sequence before extending to the top surface of the third side wall. The bottom plate is placed in a rectangular placement slot. The top surface of the bottom plate is flush with the top surface of the mounting base plate (1). Each side of the bottom plate is pressed against the inner side surface of the rectangular placement slot. The embedded block is placed in the embedded block placement seat (8), and the embedded block covers the part of all through slots located on the bottom plate. Lateral propulsion mechanism; The lateral propulsion mechanism is set on the mounting base plate (1). The lateral propulsion mechanism includes a lateral power output end and a lateral movement end. The lateral power output end is used to drive the lateral movement end to laterally squeeze or release the compression of the embedded block set in the embedded block placement seat (8), so that the embedded block is squeezed into contact or released from contact with the inner side of the second side wall. Longitudinal propulsion mechanism; The longitudinal propulsion mechanism is set on the mounting base plate (1). The longitudinal propulsion mechanism includes a mounting frame, a longitudinal power output end and a longitudinal motion end. The longitudinal power output end is set on the mounting frame, and the longitudinal motion end is set on the top surface of the bottom plate of the embedding block placement seat (8). Multi-blade slicing holder; The multi-blade slicing holder includes a base (5) and a blade. The blade is composed of multiple blades (6) arranged parallel to each other on the first surface of the base (5). The second surface of the base (5) is detachably connected to the longitudinal moving end. The longitudinal pushing mechanism is used to drive the blades (6) in the blade to insert or withdraw from the groove one by one. When the blade moves to the lower extreme point, the two sides of the blade (6) and the cutting edge are respectively located in the groove on the inner side of the first side wall, the inner side of the third side wall and the top surface of the bottom plate. Control module; both the lateral power output end and the longitudinal power output end are connected to the control module, which is used to output preset control commands to the lateral power output end and the longitudinal power output end.

2. The high-efficiency ultramicro pathological sample preparation device according to claim 1, characterized in that, The lateral propulsion mechanism includes: Lateral telescopic motor (14); The lateral telescopic motor (14) is connected to the control module. The lateral telescopic motor (14) is fixedly installed on the mounting base plate (1). The telescopic rod of the lateral telescopic motor (14) is set to point to the inner side of the second side wall. Elastic buffer mechanism; the first end of the elastic buffer mechanism is connected to the telescopic rod of the transverse telescopic motor (14); Push plate (10); push plate (10) is connected to the second end of the elastic buffer mechanism, and push plate (10) and the second side wall are arranged parallel to each other.

3. The high-efficiency ultramicro pathological sample preparation device according to claim 2, characterized in that, The buffer mechanism includes: Mounting plate (13); the first surface of the mounting plate (13) is fixedly connected to the end face of the telescopic rod of the transverse telescopic motor (14), and the mounting plate (13) is provided with at least one positioning through hole; At least one positioning rod (12); the first end of the positioning rod (12) is fixedly installed on the first surface of the push plate (10), and the second end of the positioning rod (12) is set through the first positioning through hole; Spring (11); one end of spring (11) is fixedly installed on the second surface of mounting plate (13), and the other end of spring (11) is fixedly installed on the first surface of push plate (10).

4. The high-efficiency ultramicro pathological sample preparation device according to claim 3, characterized in that, A rubber pad (9) is provided on the second surface of the push plate (10).

5. The high-efficiency ultramicro pathological sample preparation device according to claim 1, characterized in that, The longitudinal propulsion mechanism includes: Two support plates (7); the two support plates (7) are vertically fixed on the mounting base plate (1); Connecting plate; the connecting plate is set between the tops of the two support plates (7); Longitudinal telescopic motor (4); The longitudinal telescopic motor (4) is connected to the control module. The longitudinal telescopic motor (4) is fixedly installed on the top surface of the connecting plate, and the connecting plate is provided with a first through hole. The telescopic rod of the longitudinal telescopic motor (4) passes through the first through hole and points to the top surface of the bottom plate of the embedding block placement seat (8).

6. The high-efficiency ultramicro pathological sample preparation device according to claim 5, characterized in that, The high-efficiency ultramicro pathological sample preparation device also includes a rotating mechanism, which is used to drive the connecting plate to rotate.

7. The high-efficiency ultramicro pathological sample preparation device according to claim 6, characterized in that, The rotating mechanism includes: Motor mount (2); The motor mount (2) is fixedly mounted on the mounting base plate (1); Drive motor (3); The drive motor (3) is fixedly mounted on the motor mount (2), and the drive motor (3) is connected to the control module; Two second through holes and a third through hole; one second through hole connects the top two sides of a support plate (7), and the third through hole is set through the two sides of the connecting plate. The central axes of the two second through holes and the third through hole are coaxial. Two bearings; one bearing is set in a second through hole, and the outer ring of the bearing is fixedly connected to the inner wall of the second through hole. The shaft of the drive motor (3) passes through one bearing, the third through hole and another bearing in sequence. The shaft of the drive motor (3) is fixedly connected to the inner ring of each bearing and the shaft of the drive motor (3) is fixedly connected to the third through hole.

8. The high-efficiency ultramicro pathological sample preparation device according to claim 1, characterized in that, The blade (6) is a diamond cutting tool.

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