Rapid fluorescence detection device for aflatoxin in nuts

Abstract

The utility model relates to the technical field of fluorescence detection, specifically, a quick fluorescence detection device for aflatoxin of nut, which comprises a centrifugal component, the centrifugal component comprises a centrifugal shell, the side wall of the centrifugal shell is connected with a liquid inlet pipe for connecting the sample extraction solution of nut, the side wall of the centrifugal shell is connected with a liquid outlet pipe, the pipe end of the liquid outlet pipe is connected with a detection box, the detection box comprises a box cover and a box body, the fluorescence detector and the singlechip are fixedly installed on the bottom surface of the box cover, an incubation chamber is opened on the top surface of the box body, a plurality of detection chambers are also opened on the top surface of the box body, the inner side wall of each detection chamber is provided with a counterbore for installing a light emitting diode, and a marker tube is installed on the outer side wall of the box body and connected with the incubation chamber. The utility model can remove impurities, improve the purity of the sample liquid through centrifugal separation, accurately control the temperature of the incubation chamber through the temperature sensor and the electric heating sheet, and ensure that the antigen-antibody reaction is carried out under the best conditions.

Description

Technical Field

[0001] This utility model relates to the field of fluorescence detection technology, and more specifically, to a rapid fluorescence detection device for aflatoxin in nuts. Background Technology

[0002] Nuts are susceptible to aflatoxin contamination during storage, which poses a serious threat to food safety due to its high toxicity and carcinogenicity. Traditional detection methods are complex, time-consuming, and rely on large equipment, making it difficult to meet the needs of rapid on-site testing. Therefore, there is an urgent need for efficient and portable testing devices.

[0003] In the background technology of such devices, the pretreatment stage is crucial. The centrifugal pretreatment module works as follows: First, the rotating spiral conveyor blades crush and grind the nut sample, transforming the lumpy nuts into uniform powder, increasing the contact area with the extract; then, the mixture containing the powder enters the centrifuge chamber, where the spiral conveyor blades assist in creating a vortex, accelerating solid-liquid separation under centrifugal force, causing the supernatant containing toxins to precipitate rapidly, providing a pure test solution for subsequent fluorescence detection, significantly shortening the pretreatment time and improving detection efficiency.

[0004] Utility model patent CN221280922U discloses an aflatoxin detection device, including a detection assembly. The detection assembly includes: a detection box with an installation groove on the bottom inner side; grooves symmetrically located on both sides of the installation groove; and a partition on the top inner side of the detection box. In use, the device opens the door, controls the components inside the detection box via a controller, places the object to be tested on top of the detection platform, and then activates the telescopic rod to adjust the UV lamps and light sensors on both sides to a suitable height. Since the UV lamps are hinged to opposite sides of the concave frame, their angle can be adjusted. The device is then fixed with bolts. The detection platform is then placed inside the installation groove, and springs on both sides drive limiting blocks to restrict the detection platform, facilitating UV irradiation from the top for detection.

[0005] Although the aflatoxin detection device generally provides accurate and reliable results, it suffers from the following drawbacks in practical use: it lacks a pretreatment function, directly detecting the test solution, making it susceptible to impurities; and it lacks a temperature control component, meaning ambient temperature fluctuations may affect detection stability. Therefore, we propose a rapid fluorescent detection device for aflatoxin in nuts. Utility Model Content

[0006] In view of the above-mentioned defects or deficiencies in the prior art, it is desirable to provide a rapid fluorescent detection device for aflatoxin in nuts.

[0007] In a first aspect, this application provides a rapid fluorescent detection device for aflatoxin in nuts, including a centrifuge assembly. The centrifuge assembly includes a centrifuge shell. The side wall near the lower end of the centrifuge shell is connected to an inlet pipe for externally connecting nut sample extract. The side wall near the top of the centrifuge shell is also connected to an outlet pipe. The end of the outlet pipe away from the centrifuge shell is connected to a detection box. The detection box includes a matching lid and a box body. A fluorescence detector for monitoring fluorescence signals and a microcontroller for controlling the operation of the device are fixedly installed on the bottom surface of the lid. An incubation chamber for providing space for antigen-antibody reaction is opened on the top surface of the box body. Several test chambers are also opened on the top surface of the box body. The inner side walls of the test chambers are all provided with countersunk holes for installing light-emitting diodes. The incubation chamber and the test chambers are connected by a drainage groove. A labeling tube for adding markers is installed on the outer side wall of the box body and is connected to the incubation chamber. An openable and closable sealing cap is installed on the labeling tube.

[0008] According to the technical solution provided in the embodiments of this application, the centrifugal assembly further includes a rotating shaft passing through the bottom side wall of the centrifugal housing. The centrifugal housing includes a cylindrical chamber and a conical chamber that are connected vertically. A spiral conveying blade is fixedly sleeved on the outside of the rotating shaft in the cylindrical chamber. A plurality of triangular blades that are distributed in an annular pattern are fixedly connected on the outside of the rotating shaft in the conical chamber.

[0009] In this setting, the test solution is centrifuged using a centrifuge assembly to remove impurities.

[0010] According to the technical solution provided in the embodiments of this application, a limiting sleeve is fixedly installed at the center of the inner side wall of the top of the centrifuge shell, a centrifuge motor is fixedly installed at the bottom of the centrifuge shell, the output shaft of the centrifuge motor is coaxially connected to the rotating shaft, and a first shut-off valve is installed on the liquid outlet pipe.

[0011] In this setup, the centrifugal motor provides the driving force for centrifugation, while the limiting sleeve ensures stability during the centrifugation process.

[0012] According to the technical solution provided in the embodiments of this application, a plurality of positioning holes are opened at the bottom corner of the box cover, and a plurality of positioning posts are fixedly connected at the top corner of the box body, which are the same number as the positioning holes and correspond one-to-one in position. The inner diameter of the positioning holes and the outer diameter of the positioning posts are adapted to each other. When the plurality of positioning holes and the corresponding positioning posts are all engaged one-to-one, the fluorescence detector covers all the chambers to be inspected.

[0013] This setting ensures the positioning and locking between the lid and the body of the box, guaranteeing the accuracy and comprehensiveness of the testing.

[0014] According to the technical solution provided in the embodiments of this application, a second shut-off valve is installed on the box body near the incubation chamber at the location of the drainage channel, for connecting or disconnecting the connection between the incubation chamber and the drainage channel;

[0015] In this setup, the connection between the incubation chamber and the drainage channel is controlled by a second shut-off valve, allowing for flexible detection.

[0016] According to the technical solution provided in the embodiments of this application, a light-transmitting barrier film of a suitable size is installed at the port of a plurality of countersunk holes in which the light-emitting diodes are installed;

[0017] In this setup, a light-transmitting barrier film protects the LED from corrosion by the solution.

[0018] According to the technical solution provided in the embodiments of this application, a temperature sensor is fixedly installed on one side wall of the box, and the probe of the temperature sensor extends through the side wall of the box into the incubation chamber;

[0019] According to the technical solution provided in the embodiments of this application, an electric heating element is fixedly installed inside the box below the incubation chamber, and the size of the electric heating element is larger than the cross-sectional size of the incubation chamber;

[0020] In both of these settings, the temperature of the incubation chamber is controlled by a temperature sensor and an electric heating element.

[0021] According to the technical solution provided in the embodiments of this application, the microcontroller is connected to the fluorescence detector, the light-emitting diode, the temperature sensor, and the electric heating element via signal lines, and the microcontroller, the fluorescence detector, the light-emitting diode, the temperature sensor, and the electric heating element are all electrically connected to an external power source via wires.

[0022] In this setup, signals are received via signal lines and wires, and temperature control and fluorescence detection are performed.

[0023] In summary, this technical solution specifically discloses a rapid fluorescent detection device for aflatoxin in nuts, which includes a centrifugation component, an incubation chamber, a temperature sensor, and an electric heating element. Centrifugation can remove impurities and improve the purity of the test solution, while the temperature sensor and electric heating element can precisely control the temperature of the incubation chamber to ensure that the antigen-antibody reaction takes place under optimal conditions. Attached Figure Description

[0024] Other features, objects, and advantages of this application will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0025] Figure 1 This is a schematic diagram of the overall structure of the utility model;

[0026] Figure 2This is a schematic diagram of the centrifugal assembly in the utility model;

[0027] Figure 3 This is a schematic diagram of the detection box in the utility model;

[0028] Figure 4 This is a bottom view of the structure of the box cover of the utility model;

[0029] Figure 5 This is a schematic diagram of the structure of the box in the utility model;

[0030] Figure 6 For utility model Figure 5 Schematic diagram of the structure at point A;

[0031] Figure 7 This is a cross-sectional structural diagram of the box body in the utility model;

[0032] In the picture:

[0033] 1. Liquid inlet pipe;

[0034] 2. Centrifuge assembly; 21. Centrifuge shell; 22. Rotary shaft; 23. Spiral conveyor blade; 24. Triangular blade; 25. Centrifuge motor; 26. Limiting sleeve;

[0035] 3. Discharge pipe; 31. First shut-off valve;

[0036] 4. Detection box; 41. Box cover; 411. Fluorescence detector; 412. Microcontroller; 413. Positioning hole; 42. Box body; 421. Incubation chamber; 422. Drainage groove; 423. Test chamber; 424. Light-emitting diode; 425. Light-transmitting barrier film; 426. Second shut-off valve; 427. Temperature sensor; 428. Positioning post; 429. Electric heating element; 43. Marking tube. Detailed Implementation

[0037] The present application will now be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the relevant utility model and not intended to limit the scope of the utility model. Furthermore, it should be noted that, for ease of description, only the parts relevant to the utility model are shown in the accompanying drawings.

[0038] It should be noted that, unless otherwise specified, the embodiments and features described in this application can be combined with each other. This application will now be described in detail with reference to the accompanying drawings and embodiments.

[0039] Please see Figures 1-7A rapid fluorescent detection device for aflatoxin in nuts includes a centrifuge assembly 2, which includes a centrifuge housing 21. An inlet pipe 1 for receiving nut sample extract is connected to the lower side wall of the centrifuge housing 21. An outlet pipe 3 is also connected to the upper side wall of the centrifuge housing 21. A detection box 4 is connected to the end of the outlet pipe 3 away from the centrifuge housing 21. The detection box 4 includes a matching lid 41 and a box body 42. A fluorescence detector 411 for monitoring fluorescence signals and a control device are fixedly installed on the bottom surface of the lid 41. The device is powered by a microcontroller 412. The top surface of the housing 42 has an incubation chamber 421 for providing space for the antigen-antibody reaction. The top surface of the housing 42 also has several test chambers 423. The inner sidewalls of the test chambers 423 have countersunk holes for installing light-emitting diodes 424. The incubation chambers 421 and the test chambers 423 are connected by a drainage channel 422. The outer sidewall of the housing 42 has a labeling tube 43 for adding markers, which is connected to the incubation chambers 421. The labeling tube 43 has an openable and closable sealing cap.

[0040] In this embodiment, as Figure 3 As shown, the centrifuge assembly 2 also includes a rotating shaft 22 passing through the bottom side wall of the centrifuge housing 21. The centrifuge housing 21 includes a cylindrical chamber and a conical chamber that are connected vertically. A spiral conveying blade 23 is fixedly sleeved on the outside of the rotating shaft 22 in the cylindrical chamber to centrifuge the test liquid in the centrifuge housing 21 and push the supernatant to the outlet pipe 3. Several triangular blades 24 that are equidistantly distributed in a ring are fixedly connected on the outside of the rotating shaft 22 in the conical chamber to crush the nuts and mix the nuts and the extract.

[0041] Furthermore, such as Figure 3 As shown, a limiting sleeve 26 is fixedly installed on the inner side wall of the top of the centrifuge housing 21 at the top of the rotating shaft 22, which limits the position of the rotating shaft 22 and ensures its stable rotation. A centrifuge motor 25 is fixedly installed on the bottom of the centrifuge housing 21. The output shaft of the centrifuge motor 25 is coaxially connected to the rotating shaft 22 to provide power for the rotation of the rotating shaft 22 and drive the spiral conveying blade 23 and the triangular blade 24 to work. A first shut-off valve 31 is installed on the liquid outlet pipe 3 to control the flow rate of the supernatant.

[0042] Furthermore, such as Figure 3 As shown, several positioning holes 413 are opened at the bottom corner of the box cover 41, and several positioning posts 428 are fixedly connected at the top corner of the box body 42, which are the same number as the positioning holes 413 and correspond one-to-one in position. The inner diameter of the positioning hole 413 and the outer diameter of the positioning post 428 are matched to ensure that the positioning post 428 and the positioning hole 413 can be engaged one-to-one to ensure the accuracy of the detection. When several positioning holes 413 and corresponding positioning posts 428 are engaged one-to-one, the fluorescence detector 411 covers all the test chambers 423, ensuring that each test chamber 423 can be detected by the fluorescence detector 411, thus ensuring the comprehensiveness of the final data.

[0043] It is important to note that, such as Figure 3 As shown, a second shut-off valve 426 is installed on the box body 42 near the incubation chamber 421 in the drainage channel 422. It is used to connect or disconnect the connection between the incubation chamber 421 and the drainage channel 422. The second shut-off valve 426 controls the opening and closing of the incubation chamber 421 and the drainage channel 422, and can adjust the timing of the solution entering the test chamber 423 according to the reaction progress.

[0044] In this embodiment, as Figure 3 As shown, a light-transmitting barrier film 425 of a suitable size is installed at the port of several countersunk holes where LEDs 424 are installed. This film can both ensure light transmission and isolate liquids, thus protecting the LEDs 424.

[0045] Furthermore, such as Figure 3 As shown, a temperature sensor 427 is fixedly installed on one side wall of the box 42. The probe of the temperature sensor 427 extends through the side wall of the box 42 into the incubation chamber 421 to monitor the temperature inside the incubation chamber 421 in real time and ensure that the antigen-antibody reaction is carried out at a suitable temperature.

[0046] Furthermore, such as Figure 3 As shown, an electric heating element 429 is fixedly installed inside the box 42 below the incubation chamber 421. The size of the electric heating element 429 is larger than the cross-sectional size of the incubation chamber 421, which heats the incubation chamber 421 and, together with the temperature sensor 427, achieves precise control of the reaction temperature.

[0047] It is worth mentioning that, such as Figure 3 As shown, the microcontroller 412 is connected to the fluorescence detector 411, the light-emitting diode 424, the temperature sensor 427, and the electric heating element 429 via signal lines. The microcontroller 412, the fluorescence detector 411, the light-emitting diode 424, the temperature sensor 427, and the electric heating element 429 are all electrically connected to an external power supply via wires. The microcontroller controls the light-emitting diode 424 to emit laser light, triggering the fluorescent marker to emit light. It receives the signal from the fluorescence detector 411 to analyze the aflatoxin concentration, adjusts the power of the electric heating element 429 to maintain a constant temperature in the incubation chamber, and optimizes the antigen-antibody reaction efficiency.

[0048] Finally, it should be noted that the centrifugal motor 25, fluorescence detector 411, microcontroller 412, light-emitting diode 424, temperature sensor 427, electric heating element 429, and other components involved in this utility model are all general standard parts or components known to those skilled in the art. Their structures and principles can be learned by those skilled in the art through technical manuals or conventional experimental methods. In the idle space of this device, all the above-mentioned electrical components, which refer to power elements, electrical components, and the matching controller and power supply, are connected by wires. The specific connection methods should refer to the working principle of this utility model. The electrical connections between each electrical component are completed in the order of operation. The detailed connection methods are all technologies known in the art.

[0049] Working principle: In this embodiment, the rapid fluorescent detection device for aflatoxin in nuts is used such that nuts and extract enter the centrifuge assembly 2 through the inlet pipe 1. The rotating shaft 22 in the centrifuge shell 21 drives the triangular blades 24 to rotate and crush the nuts, mixing them with the extract to form the test solution. Then, the rotating spiral conveyor blades 23 provide centrifugal force to separate the test solution, causing impurities to precipitate. The supernatant flows into the incubation chamber 421 of the detection box 4 through the outlet pipe 3 and the first shut-off valve 31. At the same time, fluorescently labeled antigens or antibodies can be added to the labeling tube 43. The antigen-antibody reaction takes place in the incubation chamber 421. After the reaction, the second shut-off valve 426 is opened, and the liquid is drained through the drainage tube. The liquid flows into the test chamber 423 through the trough 422; the light-emitting diode 424 in the test chamber 423 emits a laser, causing the compound to generate a fluorescent signal. The fluorescence detector 411 detects the signal and transmits it to the microcontroller 412. At the same time, the temperature sensor 427 and the electric heating element 429 maintain the temperature of the incubation chamber 421 to ensure the reaction conditions. The positioning column 428 and the positioning hole 413 ensure that the fluorescence detector 411 accurately covers the test chamber 423. The light-transmitting barrier film 425 protects the light-emitting diode 424 from liquid interference. Finally, the microcontroller 412 quickly detects the aflatoxin content in the nuts based on the intensity of the fluorescence signal received by the fluorescence detector 411.

[0050] The above description is merely a preferred embodiment of this application and an explanation of the technical principles employed. Those skilled in the art should understand that the scope of the utility model involved in this application is not limited to the technical solutions formed by specific combinations of the above-described technical features, but should also cover other technical solutions formed by arbitrary combinations of the above-described technical features or their equivalents without departing from the inventive concept. For example, technical solutions formed by substituting the above-described features with (but not limited to) technical features with similar functions disclosed in this application.

Claims

1. A rapid fluorescent detection device for aflatoxin in nuts, characterized in that: The centrifuge assembly (2) includes a centrifuge shell (21). The centrifuge shell (21) has an inlet tube (1) connected to the lower side wall for receiving nut sample extract. The centrifuge shell (21) also has an outlet tube (3) connected to the upper side wall. A detection box (4) is connected to the end of the outlet tube (3) away from the centrifuge shell (21). The detection box (4) includes a matching lid (41) and a box body (42). A fluorescence detector (411) for monitoring fluorescence signals is fixedly installed on the bottom surface of the lid (41). The microcontroller (412) that operates the control device has an incubation chamber (421) on the top surface of the box (42) for providing space for the antigen-antibody reaction. The top surface of the box (42) also has several test chambers (423). The inner sidewalls of the test chambers (423) are all provided with recessed holes for installing light-emitting diodes (424). The incubation chamber (421) and the test chambers (423) are connected by a drainage channel (422). The outer sidewall of the box (42) is provided with a labeling tube (43) for adding markers, which is connected to the incubation chamber (421).

2. The rapid fluorescent detection device for aflatoxin in nuts according to claim 1, characterized in that: The centrifugal assembly (2) also includes a rotating shaft (22) that passes through the bottom side wall of the centrifugal housing (21). The centrifugal housing (21) includes a cylindrical chamber and a conical chamber that are connected vertically. A spiral conveying blade (23) is fixedly sleeved on the outside of the rotating shaft (22) in the cylindrical chamber. Several triangular blades (24) that are distributed in a ring at equal intervals are fixedly connected on the outside of the rotating shaft (22) in the conical chamber.

3. The rapid fluorescent detection device for aflatoxin in nuts according to claim 2, characterized in that: The top of the rotating shaft (22) is fitted with a limiting sleeve (26) fixedly installed in the center of the inner side wall of the top of the centrifuge shell (21). A centrifuge motor (25) is fixedly installed on the bottom of the centrifuge shell (21). The output shaft of the centrifuge motor (25) is coaxially connected with the rotating shaft (22). A first shut-off valve (31) is installed on the liquid outlet pipe (3).

4. The rapid fluorescent detection device for aflatoxin in nuts according to claim 1, characterized in that: The bottom corner of the box cover (41) has several positioning holes (413), and the top corner of the box body (42) is fixedly connected with several positioning posts (428) that are the same number as the positioning holes (413) and whose positions correspond one-to-one. The inner diameter of the positioning holes (413) and the outer diameter of the positioning posts (428) are compatible. When the several positioning holes (413) and the corresponding positioning posts (428) are all engaged, the fluorescence detector (411) covers all the chambers to be inspected (423).

5. The rapid fluorescent detection device for aflatoxin in nuts according to claim 1, characterized in that: The box (42) is equipped with a second shut-off valve (426) near the incubation chamber (421) in the drainage channel (422) for connecting or disconnecting the connection between the incubation chamber (421) and the drainage channel (422).

6. The rapid fluorescent detection device for aflatoxin in nuts according to claim 1, characterized in that: A light-transmitting barrier film (425) of a suitable size is installed at the port of a plurality of countersunk holes in which the light-emitting diodes (424) are installed.

7. The rapid fluorescent detection device for aflatoxin in nuts according to claim 1, characterized in that: A temperature sensor (427) is fixedly installed on one side wall of the box (42), and the probe of the temperature sensor (427) extends through the side wall of the box (42) into the incubation chamber (421).

8. The rapid fluorescent detection device for aflatoxin in nuts according to claim 7, characterized in that: An electric heating element (429) is fixedly installed inside the box (42) below the incubation chamber (421), and the size of the electric heating element (429) is larger than the cross-sectional size of the incubation chamber (421).

9. The rapid fluorescent detection device for aflatoxin in nuts according to claim 8, characterized in that: The microcontroller (412) is connected to the fluorescence detector (411), light-emitting diode (424), temperature sensor (427), and electric heating element (429) via signal lines. The microcontroller (412), fluorescence detector (411), light-emitting diode (424), temperature sensor (427), and electric heating element (429) are all electrically connected to an external power source via wires.

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