Nucleic acid extraction module and nucleic acid extraction device

Abstract

This application discloses a nucleic acid extraction module and a nucleic acid extraction device. The nucleic acid extraction module includes: a mounting frame with an internal mounting space for mounting a reagent cartridge, the reagent cartridge having a detection chamber and multiple receiving chambers; a first rotating member rotatably mounted on the mounting frame; a piston rod vertically movable and inserted into the receiving chamber of the reagent cartridge; a first driving assembly drivenly connected to the piston rod; and a second driving assembly for driving the first rotating member to rotate, causing the piston rod to switch between a first preset position and a second preset position. When the piston rod is in the first preset position, the material to be transferred can be transferred between different receiving chambers; when the piston rod is in the second preset position, the nucleic acid detection solution can be transferred from the receiving chamber to the detection chamber. This nucleic acid extraction module and device have advantages such as simple structure, few parts, low energy consumption, and low cost.

Description

Technical Field

[0001] This application belongs to the field of medical testing technology, specifically relating to a nucleic acid extraction module and a nucleic acid extraction device. Background Technology

[0002] Nucleic acid extraction devices (such as nucleic acid extractors) belong to the field of molecular detection equipment and are widely used in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry, and molecular biology research. Nucleic acid extraction devices include a nucleic acid extraction module, which comprises multiple piston rods, magnetic components, and multiple driving elements. However, the structural arrangement of these components in existing nucleic acid extraction modules is not optimal, resulting in overall drawbacks such as complex structure, high energy consumption, and high cost. Utility Model Content

[0003] The purpose of this application is to provide a nucleic acid extraction module and a nucleic acid extraction device, which have the advantages of simple structure, few parts, low energy consumption and low cost.

[0004] To achieve the above objectives, the first aspect of this application provides a nucleic acid extraction module, which includes:

[0005] The mounting frame has an internal mounting space for mounting reagent cartridges, and the reagent cartridges have a detection chamber and multiple receiving chambers.

[0006] The first rotating component is rotatably mounted on the mounting bracket;

[0007] The piston rod is vertically movable and inserted into the receiving cavity of the reagent card box;

[0008] The first drive assembly is connected to the piston rod drive.

[0009] The second driving component is used to drive the first rotating component to rotate so that the piston rod switches between a first preset position and a second preset position. When the piston rod is in the first preset position, the object to be transferred can be transferred between different receiving cavities. When the piston rod is in the second preset position, the nucleic acid detection solution can be transferred from the receiving cavity to the detection cavity.

[0010] In embodiments of this application, the plurality of receiving cavities includes a piston receiving cavity and a plurality of mixing liquid receiving cavities, the plurality of mixing liquid receiving cavities being distributed longitudinally, the piston receiving cavity being located on the same transverse side of the plurality of mixing liquid receiving cavities, the number of detection cavities being plurality, the plurality of detection cavities being distributed one-to-one with the plurality of mixing liquid receiving cavities, the central axis of the piston rod being located on one side of the central axis of the first rotating member, the first driving assembly including a first connecting member and a first driving member, the first connecting member being disposed above the piston rod and being movable vertically, the first driving member being drivenly connected to the first connecting member, and the nucleic acid extraction module further including:

[0011] The second rotating component is rotatably disposed at the bottom end of the first connecting component and connected to the piston rod, with the central axis of the piston rod located on one side of the central axis of the second rotating component.

[0012] In an embodiment of this application, a groove is formed on the outer peripheral wall of the first rotating member, spiraling upwards from the bottom end to the top end of the first rotating member; the second driving assembly includes:

[0013] The first mounting base is mounted on the mounting bracket;

[0014] The second connector is vertically movable and mounted on the first mounting base;

[0015] The slide rod has a first end connected to a second connector, and a second end that can extend into a slide groove and move along the extension direction of the slide groove. The second end of the slide rod forms a sliding contact with the side wall of the slide groove.

[0016] The second driving component is mounted on the first mounting base and drivenly connected to the second connector.

[0017] In embodiments of this application, the nucleic acid extraction module further includes:

[0018] A first guide assembly is vertically disposed on a first mounting base and is used to guide the second connector.

[0019] In embodiments of this application, the nucleic acid extraction module further includes:

[0020] A limit stop is provided on the first slider of the first guide assembly;

[0021] The detection component is mounted on the first mounting base and is used to detect the limit stop plate.

[0022] In embodiments of this application, the nucleic acid extraction module further includes:

[0023] A magnetic attachment assembly is provided on the second connector and is used to attract magnetic beads.

[0024] In an embodiment of this application, the bottom end of the chute is provided with a chute outlet for the slide rod to slide out of the chute.

[0025] In embodiments of this application, the nucleic acid extraction module further includes:

[0026] The pressure-down assembly is located at the bottom of the mounting base and is used to press down the reagent cartridge.

[0027] In embodiments of this application, the nucleic acid extraction module further includes:

[0028] The second mounting base is mounted on the mounting frame, and the first connector is vertically movable and mounted on the second mounting base;

[0029] The first driving component is mounted on the second mounting base and drivenly connected to the first connecting component;

[0030] The second guide assembly is disposed on the second mounting base and is used to guide the first connector.

[0031] A second aspect of this application provides a nucleic acid extraction device, which includes the aforementioned nucleic acid extraction module.

[0032] As can be seen from the above technical solution, the nucleic acid extraction module includes a mounting frame, a first rotating component, a piston rod, a first driving assembly, and a second driving assembly. The mounting frame has an internal mounting space for mounting reagent cartridges, and the reagent cartridges have a detection chamber and multiple receiving chambers. The first rotating component is rotatably mounted on the mounting frame. The piston rod is vertically movable and inserted into the first rotating component and extends into the receiving chamber of the reagent cartridge. The first driving assembly is driven to the piston rod. The second driving assembly is used to drive the first rotating component to rotate so that the piston rod switches between a first preset position and a second preset position. When the piston rod is in the first preset position, the material to be transferred can be transferred between different receiving chambers. When the piston rod is in the second preset position, the nucleic acid detection solution can be transferred from the receiving chamber to the detection chamber. The layout of the first rotating component, piston rod, first driving component, and second driving component of the nucleic acid extraction module is reasonable. Only one piston rod is needed to transfer the object to be transferred between different containment cavities or to transfer the nucleic acid detection solution from the containment cavity to the detection cavity. It also has the advantages of simple structure, reduced number of parts in the nucleic acid extraction module, and reduced energy consumption and manufacturing cost of the nucleic acid extraction module.

[0033] Other features and advantages of the embodiments of this application will be described in detail in the following detailed description section. Attached Figure Description

[0034] The accompanying drawings are provided to further illustrate the embodiments of this application and form part of the specification. They are used together with the following detailed description to explain the embodiments of this application, but do not constitute a limitation on the embodiments of this application. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without any inventive effort. In the drawings:

[0035] Figure 1 This is a first-view structural diagram of the nucleic acid extraction module in an embodiment of this application;

[0036] Figure 2 This is a second-view structural diagram of the nucleic acid extraction module in an embodiment of this application;

[0037] Figure 3 This is a first-view structural diagram of the second driving component in an embodiment of this application;

[0038] Figure 4 This is a schematic diagram of the second perspective structure of the second driving component in the embodiments of this application;

[0039] Figure 5 This is a partial structural diagram of the nucleic acid extraction module in an embodiment of this application;

[0040] Figure 6 This is a first-view structural diagram of the reagent cartridge in an embodiment of this application;

[0041] Figure 7 This is a second-view structural diagram of the reagent cartridge in an embodiment of this application;

[0042] Figure 8 This is a third-view structural diagram of the reagent cartridge in an embodiment of this application;

[0043] Figure 9 This is a cross-sectional schematic diagram of the reagent card box in an embodiment of this application;

[0044] Figure 10 This is a schematic diagram of the plunger structure in an embodiment of this application;

[0045] Figure 11 This is a schematic diagram of the structure of the first rotating component in an embodiment of this application;

[0046] Figure 12 This is a schematic diagram of the structure of the liquid circuit switching module in the embodiment of this application;

[0047] Figure 13 This is a third-view structural diagram of the nucleic acid extraction module in an embodiment of this application;

[0048] Figure 14 This is a schematic diagram of the structure of the third mounting base in the embodiments of this application.

[0049] Explanation of reference numerals in the attached figures

[0050] 1-Mounting bracket; 101-Mounting space; 2-Reagent cartridge; 201-Receiving cavity; 202-Reaction tube section; 203-Reagent tube section; 204-Base section; 205-Cyclic chamber; 206-Piston receiving cavity; 207-Washing solution receiving cavity; 208-Mixed solution receiving cavity; 209-Plunger cavity; 210-Pipette channel; 3-First rotating component; 301-Slide groove; 302-First slide groove outlet; 303-Second slide groove outlet; 4-First connecting component; 5-Piston rod; 6-Second drive assembly; 601-First mounting base; 602-Second connecting component; 603-Slide rod; 604-Second drive component; 7-First guide assembly; 701-First guide rail; 702-First slider; 8-Limit stop; 9-Detection component; 10-Magnetic suction assembly; 1001-Contact surface; 11-Pressing assembly; 1101-Pressing mounting component; 1102-Pressing roller; 12-Second mounting base; 13-First driving component; 14-Second guide assembly; 1401-Second guide rail; 1402-Second slider; 15-First lead screw; 16-Second lead screw; 17-Plunger; 1701-Pipette; 1702-Isolation structure; 18-Liquid circuit on / off module; 1801-Horizontal movement assembly; 1802-Longitudinal movement component; 1803-Push rod assembly; 19-Third mounting base; 1901-Mounting groove; 20-Second rotating component. Detailed Implementation

[0051] The specific embodiments of this application will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit this application.

[0052] Embodiments of this application provide a nucleic acid extraction module, such as... Figures 1-2 As shown, the nucleic acid extraction module includes:

[0053] Mounting frame 1, the interior of mounting frame 1 has an installation space 101 for mounting reagent card box 2, and the reagent card box 2 has a detection cavity and multiple receiving cavities 201.

[0054] The first rotating component 3 is rotatably mounted on the mounting bracket 1;

[0055] The piston rod 5 is vertically movable and inserted into the first rotating part 3 and extends into the receiving cavity 201 of the reagent card box 2;

[0056] The first drive assembly is driven by the piston rod 5.

[0057] The second driving component 6 is used to drive the first rotating component 3 to rotate so that the piston rod 5 switches between a first preset position and a second preset position. When the piston rod 5 is in the first preset position, the object to be transferred can be transferred between different receiving cavities 201. When the piston rod 5 is in the second preset position, the nucleic acid detection solution can be transferred from the receiving cavity 201 to the detection cavity.

[0058] Specifically, the nucleic acid extraction module in this embodiment is applicable to a nucleic acid extraction device, which also includes a reagent cartridge 2 and a liquid flow switching module 18. The nucleic acid extraction module also includes a controller, which is communicatively connected to the liquid flow switching module 18, the second drive component 6, and the first drive component. Figures 6-10 As shown, the reagent cartridge 2 includes a reagent tube section 203, a base section 204, a reaction tube section 202, and multiple plungers 17. The base section 204 is located at the bottom of the reagent tube section 203. Multiple (e.g., 11) concave-to-vertical receiving cavities 201 are formed on the reagent tube section 203 (each receiving cavity 201 can hold different types of solutions, such as washing solution, elution solution, or protease solution). A detection cavity is formed in the reaction tube section 202. A pipetting channel 210 and multiple plunger cavities 209 are formed on the base section 204. The pipetting channel 210 can connect different receiving cavities 201, or connect the receiving cavity 201 with the detection cavity. The multiple plunger cavities 209 are connected to the multiple receiving cavities 201. The plunger cavity 209 is located between the receiving cavity 201 and the pipetting channel 210 in a one-to-one correspondence configuration (in this embodiment, after the reagent cartridge 2 is installed on the reagent cartridge mounting base, the plunger cavity 209 is distributed longitudinally). The plunger 17 is disposed in the plunger cavity 209 and can move axially along the plunger cavity 209. A pipetting groove 1701 and an isolation structure 1702 are formed on the plunger 17. The pipetting groove 1701 is used to connect the pipetting channel 210 and the receiving cavity 201, and the isolation structure 1702 is used to isolate the pipetting channel 210 and the receiving cavity 201. The liquid circuit switching module 18 is used to drive the plunger 17 to move axially in the plunger cavity 209 to connect or isolate the pipetting channel 210 and the receiving cavity 201.

[0059] In one embodiment of this application, such as Figures 12-13 As shown, the liquid circuit switching module 18 includes a lateral moving component 1801, a longitudinal moving component 1802, and a push rod assembly 1803. The lateral moving component 1801 is movably disposed below the mounting bracket 1, and the longitudinal moving component 1802 is movably disposed on the lateral moving component 1801. Part of the longitudinal moving component 1802 extends into the mounting space 101. The push rod assembly 1803 is disposed on the longitudinal moving component 1802 and is used to push the plunger 17 in the plunger cavity 209 to connect or disconnect the receiving cavity 201 and the liquid transfer channel 210.

[0060] Specifically, such as Figure 14As shown, the nucleic acid extraction device also includes a third mounting base 19 that is laterally movable in the installation space 101 and is used to install the reagent cartridge 2. The third mounting base 19 has a mounting groove 1901 for installing the reagent cartridge 2, and the base portion 204 of the reagent cartridge 2 is accommodated in the mounting groove 1901. When it is necessary to connect two receiving cavities 201 (such as the washing liquid receiving cavity 207 and the piston receiving cavity 206) through the pipetting channel 210, the lateral moving assembly 1801 first performs a first lateral movement operation, so that the lateral moving assembly 1801 drives the longitudinal moving member 1802 and the push rod assembly 1803 to move laterally until the push rod assembly 1803 and the corresponding plunger cavity 209 of the washing liquid receiving cavity 207 are aligned laterally; then the longitudinal moving member 1802 performs a first longitudinal movement operation, so that the first axial end of the push rod assembly 1803 applies a first thrust to the plunger 17, so that the pipetting channel 210 and the washing liquid receiving cavity 207 are connected; after the first longitudinal movement operation is completed, the longitudinal moving member 1802 performs a reset operation, so that the push rod assembly 1803 returns to its initial position in the longitudinal direction.

[0061] After the reset operation is completed, the lateral movement assembly 1801 performs a second lateral movement operation to align the push rod assembly 1803 with the corresponding plunger cavity 209 of the piston receiving cavity 206 laterally. Then, the longitudinal movement member 1802 performs a second longitudinal movement operation so that the push rod assembly 1803 applies a first thrust to the plunger 17, thereby connecting the pipetting channel 210 and the piston receiving cavity 206. At this point, the washing fluid receiving cavity 207 and the piston receiving cavity 206 are connected, and liquid (such as washing fluid) in the washing fluid receiving cavity 207 can flow into the piston receiving cavity 206 through the pipetting channel 210. To isolate the pipetting channel 210 and the piston receiving cavity 206, the longitudinal movement member 1802 drives the push rod assembly 1803 to apply a second thrust to the plunger 17 to isolate the receiving cavity 201 and the pipetting channel 210.

[0062] In this embodiment, the multiple receiving cavities 201 include a washing solution receiving cavity 207, a piston receiving cavity 206, a lysis receiving cavity 201, and a mixed solution receiving cavity 208. During the operation of the nucleic acid extraction module, if it is necessary to transfer the material to be transferred (such as washing solution) between different receiving cavities 201 of the reagent cartridge 2, such as transferring the washing solution in the washing solution receiving cavity 207 to the lysis receiving cavity 201, when the piston rod 5 is in the first preset position (in this embodiment, when the piston rod 5 is in the first preset position, the central axis of the piston rod 5 coincides with the central axis of the piston receiving cavity 206) and has already extended to the bottom of the piston receiving cavity 206, the controller controls the liquid circuit on / off module 18 to perform a first connection operation to connect the washing solution receiving cavity 207 and the piston receiving cavity 206, and then controls the first drive assembly to drive the piston rod. As piston rod 5 rises, a negative pressure is created in piston receiving cavity 206, drawing the washing liquid from washing liquid receiving cavity 207 into piston receiving cavity 206. After the washing liquid is drawn in, the controller controls the liquid circuit switching module 18 to perform a first isolation operation to isolate washing liquid receiving cavity 207 from transfer channel 210. After the first isolation operation is completed, the controller controls the liquid circuit switching module 18 to perform a second connection operation to connect pyrolysis receiving cavity 201 and piston receiving cavity 206. Then, the controller controls the first drive assembly to drive piston rod 5 to descend. During the descent of piston rod 5, piston rod 5 applies pressure to the washing liquid in piston receiving cavity 206 to squeeze the washing liquid into pyrolysis receiving cavity 201. In this way, the transfer of the object to be transferred between different receiving cavities 201 of reagent cartridge 2 can be achieved.

[0063] After the nucleic acid extraction module has completed the elution operation and transferred the purified nucleic acid to the mixing solution container 208, and the purified nucleic acid is mixed with the lyophilized bulbs pre-stored in the mixing solution container 208 to form a nucleic acid detection solution, it is necessary to transfer the nucleic acid detection solution from the mixing solution container 208 to the detection chamber of the reaction tube 202. At this time, the piston rod 5 needs to be switched from the first preset position to the second preset position (in this embodiment, when the piston rod 5 is in the second preset position, the central axis of the piston rod 5 coincides with the central axis of the mixing solution container 208). The controller controls the first drive assembly to drive the piston rod 5 out of the piston receiving cavity 206; then it controls the second drive assembly 6 to drive the first rotating member 3 to rotate. The piston rod 5 rotates with the rotation of the first rotating member 3. Since the central axis of the piston rod 5 is located on one side of the central axis of the first rotating member 3, that is, the piston rod 5 is offset from the rotation center of the first rotating member 3, the position of the piston rod 5 on its circumferential motion trajectory also changes when the first rotating member 3 rotates, and thus it can change from the first preset position to the second preset position. After the first rotating member 3 has finished rotating, the controller controls the first drive assembly to drive the piston rod 5 to descend. During the descent of the piston rod 5, the piston rod 5 applies pressure to the nucleic acid detection solution in the mixed liquid receiving cavity 208 to squeeze the nucleic acid detection solution into the detection cavity of the reaction tube. In this way, the nucleic acid detection solution can be transferred from the mixed liquid receiving cavity 208 of the reagent cartridge 2 to the reaction tube 202.

[0064] In this embodiment, the layout of the first rotating component 3, piston rod 5, first driving component, and second driving component 6 of the nucleic acid extraction module is reasonable. Only one piston rod 5 is needed to realize the transfer of the object to be transferred between different accommodating cavities 201 of the reagent cartridge 2 or to transfer the nucleic acid detection solution from the accommodating cavity 201 of the reagent cartridge 2 to the reaction tube 202 of the reagent cartridge 2. It also has the advantages of simple structure, reduced number of parts of the nucleic acid extraction module, and reduced energy consumption and manufacturing cost of the nucleic acid extraction module.

[0065] In one embodiment of this application, the plurality of receiving cavities 201 include a piston receiving cavity 206 and a plurality of mixing liquid receiving cavities 208, the plurality of mixing liquid receiving cavities 208 are distributed longitudinally, the piston receiving cavity 206 is located on the same transverse side of the plurality of mixing liquid receiving cavities 208, the number of detection cavities is plurality, the plurality of detection cavities are distributed one-to-one with the plurality of mixing liquid receiving cavities 208, the central axis of the piston rod 5 is located on one side of the central axis of the first rotating member 3, the first driving assembly includes a first connecting member 4 and a first driving member 13, the first connecting member 4 is disposed above the piston rod 5 and can move vertically, the first driving member 13 is drivenly connected to the first connecting member 4, and the nucleic acid extraction module further includes:

[0066] The second rotating member 20 is rotatably disposed at the bottom end of the first connecting member 4 and connected to the piston rod 5. The central axis of the piston rod 5 is located on one side of the central axis of the second rotating member 20.

[0067] Specifically, in this embodiment, there are two mixing chambers 208, namely a first mixing chamber and a second mixing chamber. The piston chamber 206, the first mixing chamber, and the second mixing chamber are triangularly distributed on the reagent cartridge 2. The first mixing chamber and the second mixing chamber are located on the same lateral side of the piston chamber 206, and are aligned laterally. Correspondingly, there are two second preset positions in this embodiment (in this embodiment, regardless of whether the piston rod 5 is in a position that can extend into the first mixing chamber or the second mixing chamber, the piston rod 5 is located in the second preset position). In this embodiment, the reagent cartridge 2 has a first reaction tube section and a second reaction tube section. The first mixing chamber can communicate with the detection chamber of the first reaction tube, and the second mixing chamber can communicate with the detection chamber of the second reaction tube. The above arrangement is beneficial to improving the efficiency of nucleic acid detection.

[0068] When piston rod 5 is in the first preset position, the central axis of piston rod 5 coincides with the central axis of piston receiving cavity 206; when piston rod 5 is in the second preset position, the central axis of piston rod 5 coincides with the central axis of mixture receiving cavity 208. When it is necessary to switch the preset position of piston rod 5, the controller controls the first driving component 13 to first drive the first connecting component 4 to move upwards, and the first connecting component 4 drives the second rotating component 20 (e.g., ...). Figure 7 As shown, the second rotating member 20 (which is in the shape of a circular plate or ring) and the piston rod 5 move upward until the piston rod 5 disengages from the piston receiving cavity 206 or the mixture receiving cavity 208. The controller then controls the second drive assembly 6 to drive the first rotating member 3 to rotate. The piston rod 5 and the second rotating member 20 rotate with the rotation of the first rotating member 3 (at this time, the first connecting member 4 is stationary, i.e., the second rotating member 20 rotates relative to the first connecting member 4). Since the central axis of the piston rod 5 is located to one side of the central axis of the first rotating member 3, i.e., the piston rod 5 is offset from the rotation center of the first rotating member 3, the second rotating member 20 rotates upward. When the first rotating component 3 rotates, the position of the piston rod 5 on its circumferential motion trajectory also changes accordingly, thereby switching the preset work position; and since the central axis of the piston rod 5 is located on one side of the rotation center of the second rotating component 20, that is, the piston rod 5 is offset from the rotation center of the second rotating component 20, the second rotating component 20 will not interfere with the second rotating component 20 when the first rotating component 3 rotates with the piston rod 5; after the first rotating component 3 has finished rotating, the controller controls the first driving component 13 to drive the first connecting component 4 to descend, and the first connecting component 4 then drives the second rotating component 20 and the piston rod 5 to descend in sequence.

[0069] When the reagent cartridge 2 is in working condition, the center of the piston receiving cavity 206, the center of the first mixed liquid receiving cavity, and the center of the second mixed liquid receiving cavity are not collinear on the horizontal cross section. Therefore, a circle can be constructed based on the above three points. Furthermore, on the horizontal plane, the center of this circle, the rotation center of the first rotating member 3, the rotation center of the piston rod 5 (in this embodiment, the rotation center of the piston rod 5 is not the center of the horizontal cross section of the piston rod 5), and the rotation center of the rotating part 402 coincide. The above arrangement enables the central axis of the piston receiving cavity 206, the central axis of the first mixed liquid receiving cavity, and the central axis of the second mixed liquid receiving cavity to be on the circumferential movement trajectory of the piston rod 5. Therefore, when the first rotating member 3 rotates, it can drive the piston rod 5 to perform circumferential movement and change its circumferential position, thereby enabling the piston rod 5 to extend into the piston receiving cavity 206, the first mixed liquid receiving cavity, or the second mixed liquid receiving cavity.

[0070] In one embodiment of this application, such as Figures 3-4 As shown, a groove 301 is formed on the outer peripheral wall of the first rotating member 3, spiraling upward from the bottom end to the top end of the first rotating member 3. The second driving assembly 6 includes:

[0071] The first mounting base 601 is mounted on the mounting bracket 1;

[0072] The second connector 602 is vertically movable and mounted on the first mounting base 601;

[0073] The slide rod 603 has a first end connected to the second connector 602, and a second end that can extend into the slide groove 301 and move along the extension direction of the slide groove 301. The second end of the slide rod 603 forms a sliding contact with the side wall of the slide groove 301.

[0074] The second driving component 604 is disposed on the first mounting base 601 and is drivenly connected to the second connector 602.

[0075] Specifically, the first mounting base 601 is disposed on one side of the first rotating member 3, and part of the first mounting base 601 extends downward into the mounting space 101. The second driving member 604 can be a motor. The second driving assembly 6 also includes a first lead screw 15. The second driving member 604 is distributed vertically, and the first lead screw 15 is distributed vertically and connected to the output end of the second driving member 604. The second connecting member 602 is sleeved on the first lead screw 15 and threadedly connected to the first lead screw 15. After the second driving member 604 rotates, it drives the first lead screw 15 to rotate. The second connecting member 602 moves along the axial direction of the first lead screw 15. The second connecting member 602 can move vertically under the drive of the second driving member 604. The second connecting member 602 then moves the sliding rod 603 in the sliding groove 301. When the sliding rod 603 spirals upward along the sliding groove 301, a first frictional force is formed between the outer peripheral wall of the second end of the sliding rod 603 and the upper side wall of the sliding groove 301. This first frictional force causes the first rotating member 3 to rotate clockwise, thereby driving the piston rod 5 to move circumferentially in the clockwise direction. Conversely, when the sliding rod 603 spirals downward along the sliding groove 301, a second frictional force is formed between the outer peripheral wall of the second end of the sliding rod 603 and the upper side wall of the sliding groove 301. This second frictional force causes the first rotating member 3 to rotate counterclockwise, thereby driving the piston rod 5 to move circumferentially in the counterclockwise direction.

[0076] In one embodiment of this application, the nucleic acid extraction module further includes:

[0077] The first guide assembly 7 is vertically disposed on the first mounting base 601 and is used to guide the second connector 602.

[0078] Specifically, the first guide assembly 7 includes a first guide rail 701 and a first slider 702. The first guide rail 701 is vertically disposed on the first mounting base 601 and located on the vertical side wall of the first mounting base 601 away from the first rotating member 3. The first slider 702 is movably disposed on the first guide rail 701 and connected to the second connector 602. When the second connector 602 moves vertically, it carries the first slider 702 along the first guide rail 701. The above arrangement enhances the connection reliability and movement trajectory stability of the second connector 602, prevents the second connector 602 from deviating from its movement trajectory, and helps to improve the movement accuracy of the second connector 602.

[0079] In one embodiment of this application, the nucleic acid extraction module further includes:

[0080] A limiting stop 8 is disposed on the first slider 702 of the first guide assembly 7;

[0081] The detection component 9 is set on the first mounting base 601 and is used to detect the limit stop plate 8.

[0082] Specifically, the detection element 9 is communicatively connected to the controller. If the detection element 9 detects the limit stop 8, it means that the second connecting element 602, the first rotating element 3, and the piston rod 5 are all at their respective origin positions (at this time, the second end of the slide rod 603 is in the slide groove 301). In this case, the second connecting element 602 moves vertically a different distance from its corresponding origin position, the slide rod 603 moves a corresponding distance in the slide groove 301, and the piston rod 5 also rotates at a corresponding angle. The above settings further ensure the accuracy of the movement of the second connecting element 602, the first rotating element 3, and the piston rod 5.

[0083] In one embodiment of this application, the nucleic acid extraction module further includes:

[0084] The magnetic component 10 is disposed on the second connector 602 and is used to attract magnetic beads.

[0085] Specifically, when the nucleic acid extraction module performs the lysis operation, the magnetic beads are located in the lysis containment cavity 201 of the reagent cartridge 2. The magnetic suction component 10 is set on one side of the lysis containment cavity 201. The second connector 602 moves the magnetic suction component 10 to a position close to the bottom of the lysis containment cavity 201. The magnetic suction component 10 can adsorb the magnetic beads in the lysis containment cavity 201 so that the magnetic beads are fixed in the lysis containment cavity 201. The nucleic acid lysed from the sample cells is adsorbed on the surface of the magnetic beads, which facilitates subsequent washing or elution of the nucleic acid.

[0086] In one embodiment of this application, the magnetic suction assembly 10 has a mating surface 1001 for mating with the outer cavity wall of the receiving cavity 201.

[0087] Specifically, the magnetic attraction component 10 includes a permanent magnet (such as a samarium cobalt permanent magnet) and an armature. The permanent magnet is connected to the first connector 4, and the armature is disposed on the permanent magnet. The armature can be made of electrical pure iron and nickel-plated on its surface. The permanent magnet and the armature together form a contact surface 1001 for contacting the outer cavity wall of the receiving cavity 201. The above arrangement allows the magnetic attraction component 10 to have a larger contact area with the receiving cavity 201 (such as the fractured receiving cavity 201) where the magnetic bead is located, which is beneficial to improving the attraction effect of the magnetic attraction component 10 on the magnetic bead.

[0088] In one embodiment of this application, the bottom end of the slide groove 301 is provided with a slide groove outlet for the slide rod 603 to slide out of the slide groove 301.

[0089] Specifically, such as Figure 11As shown, in this embodiment, the first rotating member 3 is disposed on the top surface of the mounting bracket 1. When the magnetic suction assembly 10 adsorbs the magnetic beads, both the magnetic suction assembly 10 and the second connecting member 602 need to move into the mounting space 101. The setting of the chute outlet (the chute outlet is the first chute outlet 302) allows the sliding rod 603 disposed on the second connecting member 602 to disengage from the chute 301 and move into the mounting space 101, ensuring that the magnetic suction assembly 10 can reliably achieve the magnetic suction effect. The top of the chute 301 is also provided with a second chute outlet 303 for the sliding rod 603 to slide upward out of the chute 301. The setting of the second chute outlet 303 allows the sliding rod 603 to move upward along the chute 301 according to actual needs and separate from the first rotating member 3. In addition, the above settings also make it unnecessary for the nucleic acid extraction module to set a separate driving member for the magnetic suction assembly 10 to change the position of the magnetic suction assembly 10, which helps to reduce the number of parts of the nucleic acid extraction module and improve the compactness of the layout between the parts of the nucleic acid extraction module.

[0090] In one embodiment of this application, the nucleic acid extraction module further includes:

[0091] The pressing component 11 is located at the bottom of the mounting base and is used to press down the reagent card holder 2.

[0092] Specifically, the pressing assembly 11 includes a pressing mounting part 1101 and a pressing roller 1102. The pressing mounting part 1101 is disposed at the bottom end of the first mounting base 601. The pressing roller 1102 is rotatably disposed on the pressing mounting part 1101 and distributed vertically. The nucleic acid extraction module also includes a reagent cartridge mounting base for mounting the reagent cartridge 2. The pressing roller 1102 can press down on the top wall of the base part 204 when the reagent cartridge 2 is in the working state, which can further improve the stability of the reagent cartridge 2 when it is in the working state and prevent the reagent cartridge 2 from detaching from the reagent cartridge 2 mounting base.

[0093] Furthermore, the number of pressure rollers 1102 is multiple, and the multiple pressure rollers 1102 are arranged laterally at intervals on the pressure mounting component 1101, which helps to further ensure the pressure stability of the pressure assembly 11.

[0094] In one embodiment of this application, such as Figure 5 As shown, the nucleic acid extraction module also includes:

[0095] The second mounting base 12 is mounted on the mounting frame 1, and the first connecting member 4 is vertically movable and mounted on the second mounting base 12;

[0096] The first driving component 13 is mounted on the second mounting base 12 and is driven to connect with the first connecting component 4.

[0097] The second guide assembly 14 is disposed on the second mounting base 12 and is used to guide the first connector 4.

[0098] Specifically, the first driving member 13 is distributed vertically, and the nucleic acid extraction module also includes a second lead screw 16, which is distributed vertically and connected to the output end of the first driving member 13. The first connecting member 4 is sleeved on the second lead screw 16 and threadedly connected to the second lead screw 16. After the first driving member 13 rotates, it drives the second lead screw 16 to rotate, and the first connecting member 4 moves along the axial direction of the second lead screw 16.

[0099] The second guide assembly 14 includes a second guide rail 1401 and a second slider 1402. The second guide rail 1401 is vertically disposed on the vertical side wall of the second mounting base 12. The second slider 1402 is movably disposed on the second guide rail 1401 and connected to the first connector 4. When the first connector 4 moves vertically, it carries the second slider 1402 along the second guide rail 1401. The above arrangement enhances the connection reliability and motion trajectory stability of the first connector 4, prevents the first connector 4 from deviating from its motion trajectory, and helps to improve the motion accuracy of the first connector 4.

[0100] Another embodiment of this application provides a nucleic acid extraction device, which includes the nucleic acid extraction module in the above embodiment.

[0101] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0102] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between components; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0103] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0104] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. A nucleic acid extraction module, characterized in that, The nucleic acid extraction module includes: Mounting frame (1), the interior of which is formed an installation space (101) for mounting a reagent card box (2), the reagent card box (2) having a detection cavity and a plurality of receiving cavities (201); The first rotating component (3) is rotatably mounted on the mounting bracket (1); The piston rod (5) is vertically movable and inserted into the first rotating part (3) and extends into the receiving cavity (201) of the reagent card box (2); The first drive assembly is drivenly connected to the piston rod (5); The second driving component (6) is used to drive the first rotating component (3) to rotate so that the piston rod (5) switches between a first preset position and a second preset position. When the piston rod (5) is in the first preset position, the object to be transferred can be transferred between different receiving cavities (201). When the piston rod (5) is in the second preset position, the nucleic acid detection solution can be transferred from the receiving cavity (201) to the detection cavity.

2. The nucleic acid extraction module according to claim 1, characterized in that, The plurality of containment cavities (201) include a piston containment cavity (206) and a plurality of mixture containment cavities (208). The plurality of mixture containment cavities (208) are distributed longitudinally. The piston containment cavity (206) is located on the same transverse side of the plurality of mixture containment cavities (208). The number of detection cavities is plurality, and the plurality of detection cavities are distributed one-to-one with the plurality of mixture containment cavities (208). The central axis of the piston rod (5) is located on one side of the central axis of the first rotating member (3). The first driving assembly includes a first connecting member (4) and a first driving member (13). The first connecting member (4) is disposed above the piston rod (5) and can move vertically. The first driving member (13) is drivenly connected to the first connecting member (4). The nucleic acid extraction module further includes: The second rotating member (20) is rotatably disposed at the bottom end of the first connecting member (4) and connected to the piston rod (5), wherein the central axis of the piston rod (5) is located on one side of the central axis of the second rotating member (20).

3. The nucleic acid extraction module according to claim 2, characterized in that, The outer peripheral wall of the first rotating member (3) has a groove (301) that spirals upward from the bottom end of the first rotating member (3) to the top end of the first rotating member (3), and the second driving assembly (6) includes: A first mounting base (601) is disposed on the mounting bracket (1); The second connector (602) is vertically movable on the first mounting base (601); A slide rod (603) has a first end connected to the second connector (602), and a second end that can extend into the slide groove (301) and move along the extension direction of the slide groove (301). The second end of the slide rod (603) forms a sliding contact with the side wall of the slide groove (301). The second drive member (604) is disposed on the first mounting base (601) and is drivenly connected to the second connector (602).

4. The nucleic acid extraction module according to claim 3, characterized in that, The nucleic acid extraction module also includes: A first guide assembly (7) is vertically disposed on the first mounting base (601) and is used to guide the second connector (602).

5. The nucleic acid extraction module according to claim 4, characterized in that, The nucleic acid extraction module also includes: A limiting stop (8) is disposed on the first slider (702) of the first guide assembly (7); The detection element (9) is disposed on the first mounting base (601) and is used to detect the limiting baffle (8).

6. The nucleic acid extraction module according to claim 3, characterized in that, The nucleic acid extraction module also includes: A magnetic adsorption assembly (10) is disposed on the second connector (602) and is used to adsorb magnetic beads.

7. The nucleic acid extraction module according to claim 3, characterized in that, The bottom end of the slide (301) is provided with a first slide outlet (302) for the slide rod (603) to slide out of the slide (301).

8. The nucleic acid extraction module according to claim 3, characterized in that, The nucleic acid extraction module also includes: A pressing component (11) is disposed at the bottom end of the first mounting base (601) and is used to press down the reagent card holder (2).

9. The nucleic acid extraction module according to any one of claims 2-8, characterized in that, The nucleic acid extraction module also includes: The second mounting base (12) is disposed on the mounting frame (1), and the first connector (4) is disposed on the second mounting base (12) in a vertically movable manner; The first driving member (13) is disposed on the second mounting base (12) and drivenly connected to the first connecting member (4); A second guide assembly (14) is disposed on the second mounting base (12) and is used to guide the first connector (4).

10. A nucleic acid extraction device, characterized in that, The nucleic acid extraction device includes a nucleic acid extraction module according to any one of claims 1-9.

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