A filter structure for measuring the wet weight of a bacterial cell

Abstract

The utility model discloses a kind of filter structures of measuring bacterial wet weight, including container a and container b, the container b is nested in container a, the top of the container b is provided with a platform, and the bottom end of container b is provided with support net, the container b is located above the position of support net and is provided with filter membrane, the top of the container a is provided with top cover, the gasket layer is nested in the top cover and is provided with spacer layer. In the utility model, the container b and container a of telescopic can be stable storage to the bacterial body to be filtered, facilitate subsequent filtrate work, cooperate the setting of top cover, gasket layer, platform, annular groove structure, can effectively guarantee the compactness of whole container storage;The upper layer membrane of double-layer membrane is large aperture membrane, part of bacterial body can be pre-filtered, the lower layer membrane is small aperture membrane, can intercept the remaining whole bacterial body, double-layer membrane has higher bacterial body interception flow than single-layer membrane, effectively improve filtrate efficiency, ensure the accuracy of subsequent weighing result.

Description

Technical Field

[0001] This utility model relates to the field of microbial detection technology, and in particular to a filter structure for measuring the wet weight of bacterial cells. Background Technology

[0002] In numerous fields such as microbial research, fermentation engineering, and pharmaceuticals, accurate measurement of bacterial wet weight is crucial for assessing microbial growth and fermentation efficiency. Bacterial wet weight measurement reflects microbial biomass by collecting and weighing bacterial cells. Traditional centrifugation methods often suffer from drawbacks such as cumbersome operation, long processing time, low accuracy (due to residues or sediment loss during supernatant removal after centrifugation, affecting weighing results and reducing accuracy), and susceptibility to human error. Traditional filtration and weighing methods involve filtering the bacterial culture sample with filter paper and calculating the bacterial wet weight by weighing the filter paper before and after filtration. This method requires manual filtration, washing, and weighing, which may result in bacterial loss, is time-consuming, and is susceptible to environmental factors. Therefore, developing an efficient and accurate bacterial wet weight measurement technology is urgently needed.

[0003] To address the aforementioned pain points, this invention proposes an integrated device that combines centrifugation, filtration, and sealing functions. Through structural innovation and material optimization, it achieves a breakthrough in "high precision, high efficiency, and strong adaptability" for bacterial wet weight measurement. The device is used for horizontal rotor centrifugation, and the filter membrane is made of a high-flux material, capable of handling large-volume samples, samples with high bacterial concentration, or samples with high viscosity. Through innovative designs such as integrated centrifugation and filtration, efficient retention by composite filter membrane, and precise sealing to prevent leakage, this invention systematically solves the core problems of traditional methods, such as cumbersome operation, low accuracy, and poor sample adaptability. It provides a "highly efficient, accurate, and stable" tool for measuring bacterial wet weight in fields such as microbiology research and fermentation production, and has significant scientific research and industrialization value. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by proposing a filter structure for measuring the wet weight of bacterial cells.

[0005] To achieve the above objectives, the present invention adopts the following technical solution: a filter structure for measuring the wet weight of bacterial cells, comprising container a and container b, wherein container b is nested inside container a, a platform is provided on the top of container b, and a support net is provided at the bottom of container b, a filter membrane is provided inside container b above the support net, and a top cover is provided on the top of container a, wherein a gasket layer is nested inside the top cover;

[0006] The filter membrane comprises an upper membrane and a lower membrane, both of which are provided with membrane pores.

[0007] As a further description of the above technical solution:

[0008] The pore size of the upper membrane ranges from 1 to 3 μm, and the pore size of the lower membrane ranges from 0.2 to 0.6 μm. Both the upper and lower membranes are made of PES.

[0009] As a further description of the above technical solution:

[0010] A notched groove is provided on the inner wall of container b, above the filter membrane, and a sealing ring is nested in the notched groove.

[0011] As a further description of the above technical solution:

[0012] The gasket layer comprises an inner gasket made of nitrile rubber and an outer gasket made of fluororubber.

[0013] As a further description of the above technical solution:

[0014] The bottom of the outer pad is provided with an annular groove, and the platform and the annular groove are nested together.

[0015] As a further description of the above technical solution:

[0016] The outer surface of container a near the top and the inner surface of the top cover near the bottom are both provided with mating threads.

[0017] As a further description of the above technical solution:

[0018] The outer surface of the top cover is provided with anti-slip embossed texture.

[0019] As a further description of the above technical solution:

[0020] The container b is equipped with a placement seat.

[0021] This utility model has the following beneficial effects:

[0022] This filtration structure for measuring the wet weight of bacteria uses nested containers b and a to stably store the bacteria to be filtered, facilitating subsequent filtration. The top cover, gasket layer, platform, and annular groove structure effectively ensure the tightness of container a. The upper membrane of the double membrane is a large-pore membrane, which pre-filters out some bacteria, while the lower membrane is a small-pore membrane, which retains all remaining bacteria. The double membrane has a higher bacterial throughput than a single membrane, which can effectively improve filtration efficiency and ensure the accuracy of subsequent weighing results.

[0023] The entire measurement process involves fewer steps, shorter time, and simpler operation. Centrifugation can remove moisture from the bacterial precipitate to the maximum extent, reducing the impact of residual moisture on bacterial weight. It has high accuracy, fewer steps that could lead to human error, and high repeatability, greatly improving the convenience and accuracy of bacterial wet weight measurement. Moreover, its structure is more adaptable and more practical. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure of a filter structure for measuring the wet weight of bacteria proposed in this utility model;

[0025] Figure 2 This is a front view of a filter structure for measuring the wet weight of bacteria according to the present invention.

[0026] Figure 3 This invention proposes a filter structure for measuring the wet weight of bacterial cells. Figure 2 Enlarged view of A in the middle;

[0027] Figure 4 This is a schematic diagram of the placement structure of a filter structure for measuring the wet weight of bacteria proposed in this utility model;

[0028] Figure 5 The front view of the container b placed inside the placement seat is shown in the filter structure for measuring the wet weight of bacteria proposed in this utility model.

[0029] Legend:

[0030] 1. Container a; 2. Container b; 3. Platform; 4. Support mesh; 5. Filter membrane; 51. Upper membrane; 52. Lower membrane; 53. Membrane pores; 6. Notch groove; 7. Sealing ring; 8. Top cover; 9. Gasket layer; 91. Inner gasket; 92. Outer gasket; 10. Thread; 11. Annular groove; 12. Anti-slip ridge; 13. Placement seat. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model; the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. In addition, unless otherwise explicitly specified and limited, the terms "installed," "connected," and "linked" should be interpreted broadly. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. For those skilled in the art, the specific meaning of the above terms in this utility model can be understood according to the specific circumstances.

[0033] Reference Figure 1-5 This utility model provides an embodiment of a filter structure for measuring the wet weight of bacteria, including container a1 and container b2. Container b is an inner container for holding the bacterial solution to be tested and can be used for single use. Container a is a centrifugal filtrate collection container and can be reused. Container b2 is nested inside container a1. A platform 3 is provided on the top of container b2. The platform 3 allows container b2 to be stably placed inside container a1. The outer surface of container b2 can be set with volume scale lines as needed. The inner surface is sprayed with a polytetrafluoroethylene (PTFE) superhydrophobic coating, which can reduce liquid residue on the inner wall.

[0034] A support mesh 4 is provided at the bottom of container b2. A filter membrane 5 is provided inside container b2 above the support mesh 4. The support mesh 4 is in the form of a mesh plate to ensure the flow of filtrate and to provide stable support for the filter membrane 5. The filter membrane 5 consists of an upper membrane 51 and a lower membrane 52. Both the upper membrane 51 and the lower membrane 52 are provided with membrane pores 53. The upper membrane 51 is a large-pore membrane with pores 53 ranging from 1 to 3 μm in diameter, preferably 2 μm, and a thickness of 1.5 mm. The lower membrane 52 is a small-pore membrane with pores 53 ranging from 0.2 to 0.6 μm in diameter, preferably 0. The membrane has a diameter of 45μm and a thickness of 0.5mm. Both the upper membrane 51 and the lower membrane 52 are made of PES. They are stacked together to form a stable composite membrane, which allows for efficient filtrate processing during centrifugal filtration. A notch 6 is provided on the inner wall of container b2 above the filter membrane 5. A sealing ring 7 is nested inside the notch 6. The sealing ring 7 can be a ring-shaped silicone rubber ring. With the notch 6, it can be stably positioned inside container b2 to fix the filter membrane 5. This prevents the filter membrane 5 from shifting or wrinkling during centrifugation.

[0035] The container a1 is topped with a cap 8. The outer surface of container a1 near the top and the inner surface of cap 8 near the bottom both have mating threads 10. This threaded structure allows the cap 8 to be securely connected to container a1, ensuring a stable structural bond. A gasket layer 9 is nested inside the cap 8. Gasket layer 9 consists of an inner nitrile gasket 91 and an outer fluororubber gasket 92. Each gasket is 0.5 mm thick. The inner nitrile gasket is used to seal the aqueous sample. The outer fluororubber gasket is resistant to organic solvents, ensuring durability and sealing. The bottom of the outer gasket 92 is provided with an annular groove 11. The platform 3 and the annular groove 11 are nested together. The groove depth can be 0.1mm to fit the platform 3. This allows the top cover 8 and container a1 to be precisely connected after installation, providing a stable seal and preventing liquid leakage. The outer surface of the top cover 8 is provided with anti-slip ridges 12. The anti-slip ridges 12 ensure smooth screwing of the top cover 8 and prevent slipping.

[0036] Container b2 is equipped with a base 13, which serves as a base for container b2, making it convenient to place container b2 during the weighing process.

[0037] Working principle: When using the filter structure for measuring the wet weight of bacterial cells:

[0038] Place container b2 with filter membrane 5 in the placement seat 13, weigh it, record the weight as x, and then place container b2 into container a1 for collecting centrifugal supernatant.

[0039] The culture medium containing bacteria is thoroughly mixed to avoid uneven distribution of bacteria or precipitation that could lead to sampling errors. A certain volume is accurately measured and placed into container b2 with filter membrane 5. The top cover 8 is then placed on top and tightened. The platform 3 is inserted into the annular groove 11. With the help of the gasket layer 9, the tightness of the structure can be ensured.

[0040] Place container a1 with the top cover 8 on into the horizontal rotor of the centrifuge. Set the centrifugation speed and time according to the characteristics of the bacteria, start the centrifuge, and centrifuge for separation. After centrifugation, open the top cover 8, take out container b2 with filter membrane 5, place it in the placement seat 13 for weighing, and record the weight as y.

[0041] The wet weight of the bacteria, yx, is calculated based on the total weight of container b2 with filter membrane 5 and placement seat 13 measured before and after centrifugation.

[0042] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A filter structure for measuring the wet weight of bacterial cells, comprising container a (1) and container b (2), characterized in that: The container b (2) is nested inside the container a (1). The top of the container b (2) is provided with a platform (3), and the bottom of the container b (2) is provided with a support net (4). A filter membrane (5) is provided inside the container b (2) above the support net (4). The top of the container a (1) is provided with a top cover (8), and a gasket layer (9) is nested inside the top cover (8). The filter membrane (5) comprises an upper membrane (51) and a lower membrane (52), and both the upper membrane (51) and the lower membrane (52) are provided with membrane pores (53).

2. The filter structure for measuring the wet weight of bacterial cells according to claim 1, characterized in that: The pore size of the membrane pores (53) on the upper membrane (51) ranges from 1 to 3 μm, and the pore size of the membrane pores (53) on the lower membrane (52) ranges from 0.2 to 0.6 μm. Both the upper membrane (51) and the lower membrane (52) are made of PES.

3. The filter structure for measuring the wet weight of bacterial cells according to claim 1, characterized in that: A notch (6) is provided on the inner wall of the container b (2) above the filter membrane (5), and a sealing ring (7) is nested inside the notch (6).

4. The filter structure for measuring the wet weight of bacterial cells according to claim 1, characterized in that: The gasket layer (9) comprises an inner gasket (91) made of nitrile material and an outer gasket (92) made of fluororubber material.

5. The filter structure for measuring the wet weight of bacterial cells according to claim 4, characterized in that: The bottom of the outer pad (92) is provided with an annular groove (11), and the platform (3) and the annular groove (11) are nested together.

6. The filter structure for measuring the wet weight of bacterial cells according to claim 1, characterized in that: The outer surface of the container a (1) near the top and the inner surface of the top cover (8) near the bottom are both provided with mating threads (10).

7. The filter structure for measuring the wet weight of bacterial cells according to claim 1, characterized in that: The outer surface of the top cover (8) is provided with anti-slip ridges (12).

8. The filter structure for measuring the wet weight of bacterial cells according to claim 1, characterized in that: The container b (2) is equipped with a placement seat (13).

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