Culture dish for assisted reproduction egg sorting

Abstract

The utility model discloses a culture dish for assisted reproductive egg picking, culture dish includes dish seat, the dish seat includes the bottom disc and the side plate surrounded on the bottom disc, the bottom disc is combined with the side plate and forms the accommodation space, the boss is set up in the inner surface of bottom disc, the boss is used for guiding oocyte to gather on its surface. The utility model discloses through setting up the boss on the bottom disc of dish seat and setting up the angle between the outer side of boss and bottom disc is greater than 90 degrees, like this, through setting up the boss on the bottom disc of dish seat and setting up the angle between the outer side of boss and bottom disc is greater than 90 degrees, the embryo teacher can place the ovum on the outer side of boss, only needs single -handed operation to peel the egg needle and carries out the egg peeling, need not to hold culture dish, greatly reduced the operation difficulty, improved the convenience of operation, makes the embryo teacher to be able to more easily complete the egg peeling work, even long -time operation is not easy to produce fatigue.

Description

Technical Field

[0001] This utility model belongs to the field of assisted reproductive culture dish technology, specifically relating to a culture dish for egg retrieval in assisted reproduction. Background Technology

[0002] In the field of assisted reproduction, 100mm round culture dishes are commonly used for oocyte retrieval. During oocyte retrieval, to facilitate the rapid removal of granulosa cells and tissues adhering to the oocytes, embryologists typically tilt the culture dish with one hand while using a dissecting needle to place the oocytes to be processed on the tilted surface for dissection and cutting. The process requires periodically returning the oocytes to the submerged state to maintain warmth. Because oocytes are highly sensitive to temperature, operating on a tilted surface can sometimes expose the oocytes above the constant-temperature liquid surface, causing oocyte degeneration and apoptosis. Furthermore, manually tilting the culture dish cannot guarantee a consistent slope and affects microscopic observation, significantly reducing operational efficiency. The prolonged oocyte retrieval process also leaves embryologists extremely fatigued. Utility Model Content

[0003] The purpose of this invention is to overcome the shortcomings of the prior art, which is that the culture dish cannot guarantee a consistent slant and affects the observation effect under the microscope, resulting in a significant reduction in operational efficiency. This invention provides a culture dish for egg retrieval in assisted reproduction.

[0004] To achieve the above objectives, the technical solution of this utility model is: a culture dish for assisted reproductive egg retrieval, comprising:

[0005] A dish holder, the dish holder including a base and side plates surrounding the base, the base and side plates being combined to form an accommodating space;

[0006] A boss is provided on the inner surface of the chassis, and the boss is used to guide oocytes to gather on its surface.

[0007] Furthermore, the outer side of the boss includes at least one inclined surface, and the angle between the inclined surface and the chassis is greater than 90 degrees.

[0008] Furthermore, the inclined plane includes:

[0009] The first inclined surface is connected to the chassis;

[0010] The second inclined plane is connected to the first inclined plane; wherein the angle between the second inclined plane and the chassis is greater than the angle between the first inclined plane and the chassis.

[0011] Furthermore, the angle between the first inclined plane and the chassis is 135 degrees; the angle between the second inclined plane and the chassis is 175 to 176 degrees.

[0012] Furthermore, the height between the top of the first inclined surface and the chassis is 2.5 mm, and the height between the top of the second inclined surface and the top of the first inclined surface is 1 mm.

[0013] Furthermore, the center of the boss is coaxial with the center of the chassis.

[0014] Furthermore, it also includes a lid that can be placed on the dish base.

[0015] Furthermore, the lid is a transparent lid, and the base is a transparent base.

[0016] Compared with the prior art, the beneficial effects of this utility model are: by setting a protrusion on the base of the petri dish and setting the angle between the outer side of the protrusion and the base to be greater than 90 degrees, the embryologist can place the eggs on a fixed inclined surface and remove the eggs with only one hand by operating the egg removal needle, without having to hold the culture dish, which greatly reduces the difficulty of operation, improves the convenience of operation, and allows the embryologist to complete the egg removal work more easily, and is less likely to cause fatigue even after long-term operation. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1 This is a schematic diagram of the structure of the petri dish provided by this utility model;

[0019] Figure 2 This is a schematic diagram of the structure of the dish holder provided by this utility model;

[0020] Figure 3 This is a side view of the dish holder provided by this utility model;

[0021] Figure 4 yes Figure 3 Sectional view of AA;

[0022] Figure 5 This is a diagram illustrating the use of the egg-removing needle.

[0023] Explanation of icon numbers: 10. Dish base; 11. Base plate; 12. Side plate; 20. Dish lid; 30. Boss; 31. Inclined surface; 311. First inclined surface; 312. Second inclined surface; 40. Egg stripping needle. Detailed Implementation

[0024] The embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the embodiments of the present invention, and should not be construed as limiting the present invention.

[0025] In the description of the embodiments of this utility model, it should be understood that if the embodiments of this utility model involve directional indications, such as up, down, left, right, front, back, inside, outside, etc., the orientation or positional relationship of the indications is based on the orientation or positional relationship shown in the drawings. This is only for the convenience of describing the embodiments of this utility model and simplifying the description, and is not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, it should not be construed as a limitation of this utility model.

[0026] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of embodiments of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0027] In this embodiment of the invention, unless otherwise explicitly specified and limited, terms such as "installation," "connection," "joining," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part of a structure. They can be mechanical or electrical connections. They can be direct connections or indirect connections through an intermediate medium, and can represent the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this embodiment of the invention based on the specific circumstances.

[0028] like Figure 1 As shown in the embodiment of this application, a culture dish for assisted reproductive egg retrieval is provided, including a dish base 10 and a dish lid 20. Both the dish base 10 and the dish lid 20 are made of transparent plastic. In use, culture medium and oocytes are injected into the dish base 10, and the dish lid 20 is placed on the dish base 10 to prevent external contaminants from entering, while maintaining the culture medium in the dish base 10 at a suitable temperature and humidity, and reducing liquid evaporation.

[0029] like Figure 2As shown, the dish holder 10 further includes a base 11 and side plates 12 surrounding the base 11. The base 11 is circular, and the side plates 12 are arranged in a ring around the outer periphery of the base 11. The base 11 and the side plates 12 combine to form a receiving space, in which the culture medium is stored. A protrusion 30 is provided on the inner surface of the base 11. The protrusion 30 is used to provide an operating platform for oocyte retrieval and guides the oocytes to gather on its surface. In this way, a slope 31 is formed between the outer surface of the protrusion 30 and the base 11, which facilitates the placement of oocytes in the culture dish onto the slope 31 for oocyte retrieval.

[0030] In routine procedures, first place the culture dish in a 37°C plate for half an hour to maintain a constant temperature. Then, pour the obtained follicular fluid into the dish holder 10. Following the usual amount of follicular fluid retrieved, after pouring it into the dish holder 10, the follicular fluid should just cover the outer surface of the protrusion 30. When removing the oocytes, simply drag the oocytes gently to the outer surface of the protrusion 30. This allows the oocytes to be removed while remaining immersed in the constant-temperature fluid, thus ensuring the moisture and viability of the oocytes throughout the entire removal process.

[0031] Combination Figure 3 and Figure 4 In one embodiment, the inclined surface 31 includes a first inclined surface 311 and a second inclined surface 312. The first inclined surface 311 is connected to the base plate 11. During oocyte removal, the oocyte can be gently dragged from inside the base plate 11 along the transition connection between the first inclined surface 311 and the base plate 11 onto the first inclined surface 311 for oocyte removal. The second inclined surface 312 is connected to the first inclined surface 311. The angle between the second inclined surface 312 and the base plate 11 is greater than the angle between the first inclined surface 311 and the base plate 11. This allows the first inclined surface 311 to facilitate oocyte removal while keeping the oocyte in an infiltrated state. The second inclined surface 312 is used to support the oocyte. The gentle inclined surface 31 makes it easier for the oocyte to spread out flat, preventing it from slipping and making it easier to cut and peel during processing.

[0032] It should be understood that the number of inclined planes 31 in this application can be set to multiple, such as three or four, and can be adjusted according to actual needs.

[0033] In a specific embodiment, the angle between the first inclined surface 311 and the base plate 11 is 135 degrees, and the height between the top of the first inclined surface 311 and the base plate 11 is 2.5 mm. This facilitates the dragging of oocytes from the culture medium onto the first inclined surface 311 for oocyte removal. The angle between the second inclined surface 312 and the base plate 11 is 175 to 176 degrees, and the height between the top of the second inclined surface 312 and the top of the first inclined surface 311 is 1 mm. This facilitates the flattening and spreading of oocytes, thereby facilitating subsequent operations.

[0034] In this application, the center of the protrusion 30 is coaxial with the center of the base 11, and the outer periphery of the protrusion 30 is also a circular structure, that is, the protrusion 30 is located at the center of the base 11. In this way, oocytes at various angles within the base 11 can be quickly dragged onto the protrusion 30.

[0035] The integral connection between the boss 30 and the base plate 11 ensures that the position of the boss 30 is fixed, thus extending the service life of the petri dish.

[0036] like Figure 5 As shown, in the culture dish of this application, during the egg removal process, the egg removal needle 40 can be easily operated by simply placing the dish base 10 flat on the constant temperature plate. The fixed protrusion 30 eliminates the need for hand-eye coordination during the operation, reducing the workload of embryologists and making the process more standardized, the temperature more stable, and the observation results clearer, thus leading to better egg removal results.

[0037] The petri dish in this application has the following advantages:

[0038] 1. Ease of Operation: Traditional oocyte retrieval requires embryologists to hold the culture dish and use both hands to retrieve the eggs, which is difficult and tiring. This application, however, by incorporating a protrusion 30 and an inclined surface 31 within the dish holder 10, allows the embryologist to place the eggs on the fixed inclined surface 31 and perform the retrieval with only one hand using the retrieval needle 40. This eliminates the need to hold the culture dish, significantly reducing the difficulty and improving the ease of operation. Embryologists can more easily complete the oocyte retrieval process, and are less prone to fatigue even during prolonged operation.

[0039] 2. Temperature Stability: During oocyte removal, the oocytes require extremely high temperature stability. Previously, when handling the culture dish by hand, temperature fluctuations in the hand and movement during the process could lead to temperature instability within the dish, affecting oocyte viability. In this application, the culture dish lid 20 is placed on the dish base 10 during use, effectively preventing external contaminants from entering while maintaining a suitable temperature and humidity for the culture medium within the base 10, reducing liquid evaporation. The fixed slant 31 also ensures that liquid can continuously soak onto the slant 31 during oocyte removal, providing a stable temperature environment for the oocytes and ensuring they maintain good viability throughout the removal process, thereby improving the success rate of oocyte removal.

[0040] 3. Clarity of Observation: A clear field of view is crucial for embryologists to operate accurately when performing oocyte retrieval under a microscope. The culture dish in this application is made of transparent plastic, and the fixed slant 31 facilitates focus adjustment under the microscope. After the oocytes are placed on the slant 31, the embryologist can observe the morphology and structure of the oocytes more clearly, and perform cutting and retrieval operations more accurately, avoiding errors caused by a blurred field of view, and further improving the quality and effectiveness of oocyte retrieval.

[0041] 4. Standardized Egg Removal: Due to the fixed structural design of the culture dish in this application, the egg removal process is more standardized. Embryologists can perform egg removal according to a unified procedure and method, reducing differences in egg removal results caused by individual operating habits, improving the overall quality and consistency of the egg removal operation, and thus contributing to a higher success rate of assisted reproduction.

[0042] In summary, this application significantly improves the oocyte removal effect in multiple aspects, such as ease of operation, temperature stability, clear observation, and standardized oocyte removal, by optimizing the structural design of the culture dish, thus providing strong technical support for the development of assisted reproductive technology.

[0043] The above are merely preferred embodiments of the present utility model and are not intended to limit the present utility model. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A culture dish for egg retrieval in assisted reproduction, characterized in that, include: The dish holder (10) includes a base (11) and a side plate (12) surrounding the base (11), wherein the base (11) and the side plate (12) are combined to form a receiving space; A boss (30) is provided on the inner surface of the chassis (11), the boss (30) being used to guide oocytes to gather on its surface.

2. The culture dish for assisted reproductive egg retrieval according to claim 1, characterized in that, The outer side of the boss (30) includes at least one inclined surface (31), and the angle between the inclined surface (31) and the chassis (11) is greater than 90 degrees.

3. The culture dish for assisted reproductive egg retrieval according to claim 2, characterized in that, The inclined plane (31) includes: The first inclined surface (311) is connected to the chassis (11); The second inclined plane (312) is connected to the first inclined plane (311); wherein the angle between the second inclined plane (312) and the chassis (11) is greater than the angle between the first inclined plane (311) and the chassis (11).

4. The culture dish for assisted reproductive egg retrieval according to claim 3, characterized in that, The angle between the first inclined plane (311) and the chassis (11) is 135 degrees; the angle between the second inclined plane (312) and the chassis (11) is 175 to 176 degrees.

5. The culture dish for assisted reproductive egg retrieval according to claim 4, characterized in that, The height between the top of the first inclined surface (311) and the chassis (11) is 2.5 mm, and the height between the top of the second inclined surface (312) and the top of the first inclined surface (311) is 1 mm.

6. The culture dish for assisted reproductive egg retrieval according to any one of claims 1 to 5, characterized in that, The center of the boss (30) is coaxial with the center of the chassis (11).

7. The culture dish for assisted reproductive egg retrieval according to any one of claims 1 to 5, characterized in that, Also includes: The lid (20) can be placed on the dish base (10).

8. The culture dish for assisted reproductive egg retrieval according to claim 7, characterized in that, The lid (20) is a transparent lid (20), and the base (10) is a transparent base (10).

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