An all-inclusive synthesis apparatus for polypeptide production

Abstract

This application discloses a fully enclosed synthesis apparatus for peptide production, comprising a raw material storage module, a transfer module, and a synthesis reaction module. The raw material storage module includes several independent storage areas; each storage area is equipped with a discharge pipe, and the discharge pipe is equipped with a first regulating valve. The transfer module is equipped with a temporary storage tank. The synthesis reaction module includes several independent reaction zones, which are connected in series via conveying pipes. The first-stage reaction zone is equipped with a feed pipe, and the last-stage reaction zone is equipped with a discharge pipe. The feed pipe, discharge pipe, and each conveying pipe are equipped with a second regulating valve. The discharge pipe is also connected to a drying module. The synthesis apparatus also includes a controller, which is electrically connected to the first regulating valve, the transfer module, and the second regulating valve. Compared with the prior art, this application realizes fully enclosed material transfer and feeding operations, which not only reduces the labor intensity of workers but also effectively avoids raw material contamination and improves product quality.

Description

Technical Field

[0001] This application relates to the field of peptide production equipment technology, specifically to a fully enclosed synthesis apparatus for peptide production. Background Technology

[0002] Peptide production involves a variety of raw materials. In existing technologies, raw materials are generally transferred to the reaction vessel by manual feeding. This method is not only labor-intensive, but also has poor sealing properties during the transfer process. All raw materials are in an open environment and are easily contaminated by microorganisms, which can lead to substandard product quality. Utility Model Content

[0003] The main objective of this application is to provide a fully enclosed synthetic apparatus for peptide production, which aims to overcome the shortcomings of poor enclosure in the prior art.

[0004] This application achieves the above objectives through the following technical solutions: A fully automated peptide synthesis device, comprising, The raw material temporary storage module includes several independent storage areas; each storage area is equipped with a discharge pipe, and the discharge pipe is equipped with a first regulating valve. A transfer module, wherein a temporary storage tank is provided on the transfer module; The synthesis reaction module includes several independent reaction zones, each corresponding to a different reaction step. The reaction zones are connected in series via feed pipes according to the order of the reaction process. The first-stage reaction zone is equipped with a feed pipe for connecting to a temporary storage tank, and the last-stage reaction zone is equipped with a discharge pipe. A second regulating valve is provided on the feed pipe, the discharge pipe, and each feed pipe. A drying module, which is connected to the discharge pipe; The controller is electrically connected to the first regulating valve, the transfer module, and the second regulating valve.

[0005] Optionally, the raw material temporary storage module includes several separate raw material storage tanks, and each of the raw material storage tanks is provided with a discharge pipe at the bottom.

[0006] Optionally, the transfer module includes several AGV transfer trolleys, each of which is equipped with a temporary storage tank. The top of the temporary storage tank is equipped with a feeding pipe, and the AGV transfer trolley is also equipped with a discharge pump that communicates with the bottom of the temporary storage tank. The outlet end of the discharge pump is also equipped with a conveying pipe.

[0007] Optionally, each of the AGV transfer vehicles is also equipped with a robotic arm, which is equipped with a guide hose. The guide hose is connected to the feeding pipe and the conveying pipe respectively. Both the feeding pipe and the conveying pipe are equipped with regulating valves for controlling their on / off states.

[0008] Optionally, the synthesis reaction module includes several reaction vessels, and each reaction vessel is connected in series in sequence through a feed pipe according to the order of the reaction process.

[0009] Optionally, the synthesis device also includes several barcode scanners, and each of the AGV transfer trolleys has an identification code on its robotic arm.

[0010] Optionally, the drying module includes a drying chamber with a feed inlet connected to the discharge pipe at the top; a heating module is also provided inside the drying chamber; a condenser is also connected to the top of the drying chamber via an exhaust pipe, and a vacuum pump is provided on the exhaust pipe.

[0011] Optionally, the heating module includes several electric heating tubes, each of which is evenly arranged inside the drying oven, and each of the heating tubes is also provided with several heat dissipation fins on its surface.

[0012] Optionally, the drying module further includes a weighing module, on which the drying chamber is mounted; the weighing module is electrically connected to the controller.

[0013] Optionally, the controller may include an industrial control computer and a PLC that are connected in communication.

[0014] Compared with the prior art, this application has the following beneficial effects: This application includes a raw material storage module, a transfer module, and a synthesis reaction module. The raw material storage module includes several independent storage areas; each storage area is equipped with a discharge pipe, and the discharge pipe is equipped with a first regulating valve. The transfer module is equipped with a temporary storage tank. The synthesis reaction module includes several independent reaction zones, each reaction zone corresponding to a different reaction step. According to the sequence of the reaction process, the reaction zones are connected in series via conveying pipes. The first-stage reaction zone is equipped with an inlet pipe for connecting to the temporary storage tank, and the last-stage reaction zone is equipped with a discharge pipe. The inlet pipe, discharge pipe, and each conveying pipe are equipped with a second regulating valve. The discharge pipe is also connected to a drying module. The synthesis device also includes a controller, which is electrically connected to the first regulating valve, the transfer module, and the second regulating valve. In use, the material transfer program is set by the controller. The transfer module moves to the raw material storage module and connects with the discharge pipe of the corresponding storage area. Then the corresponding first regulating valve is opened to realize the extraction of raw materials. After the extraction is completed, the transfer module moves to the area where the synthesis reaction module is located and connects with the feed pipe. The second regulating valve is opened to complete the feeding operation of raw materials. The peptide synthesis reaction then takes place in the synthesis reaction module, and the final product is obtained after drying in the drying module. Compared with existing technologies, this application eliminates the need for manual feeding and material handling, effectively reducing the labor intensity of workers; This application also realizes the automatic feeding of raw materials, which helps to improve the accuracy of feeding and thus improve product quality; Secondly, in the raw material sealed storage and raw material temporary storage module of this application, the temporary storage tank on the transfer module is connected to the corresponding storage area in a sealed manner through pipelines. At the same time, during the feeding process, the temporary storage tank is also connected to the synthesis reaction module in a sealed manner. Therefore, the raw material transfer and feeding operation is fully enclosed, which maximizes the system's sealing performance, prevents the raw materials from coming into contact with the outside air and thus avoiding contamination, and ensures the cleanliness of the raw materials. At the same time, it also ensures the cleanliness of the final product, which is conducive to improving product quality. Finally, the synthesis reaction module can separate different reaction steps by setting up multiple independent reaction zones. This setup enables automated reaction synthesis production, which is beneficial to improving the synthesis efficiency of peptides. On the other hand, different auxiliary reagents are required in different steps. By separating different reaction steps, the influence of auxiliary reagent residues on subsequent steps can be avoided. By improving the purity of the reaction, the reaction efficiency and the quality of the final product can be improved. Attached Figure Description

[0015] Figure 1 A schematic diagram of the structure of a fully automated peptide synthesis device provided in this application embodiment; Figure 2 This is a schematic diagram of the transfer module. Figure 3 This is a schematic diagram of the drying module. Reference numerals: 1-Raw material temporary storage module, 2-Discharge pipe, 3-First regulating valve, 4-Transfer module, 5-Temporary storage tank, 6-Synthesis reaction module, 7-Drying module, 8-Controller, 9-Feeding pipe, 10-Infeed pipe, 11-Discharge pipe, 12-Second regulating valve, 13-Feeding pipe, 14-Discharge pump, 15-Conveying pipe, 16-Robotic arm, 17-Guide hose, 18-Regulating valve, 19-Code scanner, 20-Weighing module, 101-Raw material storage tank, 401-AGV transfer trolley, 601-Reaction vessel, 701-Drying oven, 702-Exhaust pipe, 703-Condenser, 704-Vacuum pump, 705-Heating tube, 706-Heat dissipation fins.

[0016] The purpose, features, and advantages of this application will be further explained in conjunction with the embodiments and with reference to the accompanying drawings. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] It should be noted that all directional indicators (such as up, down, left, right, front, back, etc.) in this utility model embodiment are only used to explain the relative positional relationship and movement of each component in a certain specific posture (as shown in the figure). If the specific posture changes, the directional indicator will also change accordingly.

[0019] In this utility model, unless otherwise explicitly specified and limited, the terms "connection," "fixing," etc., should be interpreted broadly. For example, "fixing" can mean a fixed connection, a detachable connection, or an integral part; it can mean a mechanical connection or an electrical connection; it can mean a direct connection or an indirect connection through an intermediate medium; it can mean the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0020] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions. Taking "robot coordinate system and / or m" as an example, it includes a robot coordinate system solution, an m solution, or a solution where both the robot coordinate system and m are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0021] Implementation Method 1 Reference Figures 1 to 3 This embodiment is an optional embodiment of this application, which discloses a fully automated peptide synthesis device, including a raw material temporary storage module 1. The raw material temporary storage module 1 includes several raw material storage tanks 101 that are separated from each other, and the structures of each raw material storage tank 101 are exactly the same. Each of the raw material storage tanks 101 is provided with a discharge pipe 2 at the bottom, and each of the discharge pipes 2 is provided with a first regulating valve 3 for controlling its on / off state. The synthesis device also includes a transfer module 4, which includes a plurality of AGV transfer carts 401. Each AGV transfer cart 401 is equipped with a temporary storage tank 5, which is used to temporarily store raw materials. It should be noted that each AGV transfer cart 401 is only used to transfer one specific raw material to avoid mixing between different raw materials. Each of the temporary storage tanks 5 is provided with a feeding pipe 13 at the top, which is used to feed the received raw materials into the temporary storage tank 5; at the same time, the AGV transfer trolley 401 is also provided with a discharge pump 14 that is connected to the bottom of the temporary storage tank 5, and the outlet end of the discharge pump 14 is also provided with a conveying pipe 15. Each of the AGV transfer trolleys 401 is also equipped with a robotic arm 16, and the robotic arm 16 is equipped with a material guiding hose 17. The material guiding hose 17 is connected to the feeding pipe 13 and the conveying pipe 15 respectively. The feeding pipe 13 and the conveying pipe 15 are both equipped with regulating valves 18 for controlling their on / off states. In actual operation, the robotic arm 16 controls the material guiding hose 17 to connect with the discharge pipe 2 located at the bottom of the raw material storage tank 101. Then, the regulating valve 18 and the first regulating valve 3 control the connection status of the entire pipeline. When feeding, the feeding pipe 13 is kept unobstructed, while the conveying pipe 9 is closed. The opposite is true when discharging. The above structure enables automated pipeline connection, further improving production efficiency while reducing the workload of workers.

[0022] The synthesis apparatus also includes a synthesis reaction module 6, which includes several reaction vessels 601. Each reaction vessel 601 is connected in series in the order of the reaction process, and each reaction zone is connected in series through a feed pipe 9. Each feed pipe 15 is also equipped with a pump for transporting reactants. According to the sequence of the reaction process, the reactor 601 corresponding to the first step reaction is the first stage reactor 601, and the reactor 601 corresponding to the last step reaction is the last stage reactor 601. A feed pipe 10 is connected to the first stage reactor 601, and a discharge pipe 11 is provided on the last stage reactor 601. A second regulating valve 12 is provided on each of the feed pipes 9, including the feed pipe 10, the discharge pipe 11, and each of the first and second stages of the conveying pipes 9; During use, the raw materials stored in the temporary storage tank 5 are fed into the first-stage reactor 601 through the feed pipe 10, and then all the second regulating valves 12 are closed to form a closed reaction environment. After the first-stage reactor 601 completes the corresponding reaction steps, the second regulating valve 12 between the first-stage reactor 601 and the second-stage reactor 601 is opened, and the semi-finished product generated in the first-stage reactor 601 is completely transferred to the second-stage reactor 601 by a pump. Then, the second regulating valve 12 is closed and the corresponding reaction is carried out, while the first-stage reactor 601 receives raw materials again. The above setup enables automated reaction synthesis production, which improves the efficiency of peptide synthesis. On the other hand, different auxiliary reagents are required in different steps. By separating different reaction steps, the influence of auxiliary reagent residues on subsequent steps can be avoided. By improving the purity of the reaction, the reaction efficiency and the quality of the final product can be improved.

[0023] Furthermore, each raw material storage tank 5 and the first-stage reactor 601 is equipped with a barcode scanner 19, and each of the AGV transfer carts 401 has an identification code on its robotic arm 16, preferably a QR code. The barcode scanner 19 can perform directional identification of the AGV transfer cart 401, thereby avoiding mixed loading of raw materials and improving the accuracy of raw material transportation. Secondly, the QR code recognition time can also record the entire raw material transportation process in real time, thereby automatically collecting various raw data, realizing full-chain digital traceability, and eliminating data fraud.

[0024] Furthermore, the synthesis apparatus also includes a drying module 7, which includes a drying chamber 701. The top of the drying chamber is provided with a feed inlet connected to the discharge pipe 11, and the discharge pipe 11 is also provided with a regulating valve 18. The drying oven 701 is also equipped with a heating module; the heating module includes a plurality of electric heating tubes 705, each of the heating tubes 705 being evenly arranged in the drying oven 701, and each of the heating tubes 705 having a plurality of heat dissipation fins 706 on its surface. The top of the drying oven 701 is also connected to a condenser 703 via an exhaust pipe 702, and a vacuum pump 704 is installed on the exhaust pipe 702; The drying module 7 further includes a weighing module 20, which is preferably an electronic weighing device in the prior art; the drying chamber 701 is placed on the weighing module 20; During the drying process, as the moisture evaporates continuously, the weight inside the drying chamber 701 will decrease continuously. A standard parameter can be set by combining the parameters such as the amount of material input into the drying chamber 701. When the actual weight parameter obtained by the weighing module 20 is equal to the standard parameter, it can be determined that the drying has been fully achieved, and the drying module can be turned off, which helps to simplify the working procedure of the entire equipment.

[0025] Furthermore, the synthesis device also includes a controller 8, which includes an industrial control computer and a PLC connected in communication. The PLC is electrically connected to each regulating valve 18 and the weighing module 20, and each of the AGV transfer carts 401 is connected in communication with the controller 8 through a wireless communication module.

[0026] The above are merely preferred embodiments of this application and do not limit the patent scope of this application. Any equivalent structural or procedural transformations made using the content of this application's specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this application.

Claims

1. A fully enclosed synthetic apparatus for peptide production, characterized in that, include, The raw material temporary storage module (1) includes several independent storage areas; each of the storage areas is provided with a discharge pipe (2), and the discharge pipe (2) is provided with a first regulating valve (3); A transfer module (4) is provided with a temporary storage tank (5); The synthesis reaction module (6) includes several independent reaction zones, each of which corresponds to a different reaction step. According to the order of the reaction process, each reaction zone is connected in series through a feed pipe (9). The first-stage reaction zone is provided with a feed pipe (10) for connecting to the temporary storage tank (5), and the last-stage reaction zone is provided with a discharge pipe (11). The feed pipe (10), the discharge pipe (11) and each feed pipe (9) are all provided with a second regulating valve (12). Drying module (7), which is connected to the discharge pipe (11); The controller (8) is electrically connected to the first regulating valve (3), the transfer module (4), and the second regulating valve (12), respectively.

2. The fully enclosed synthesis apparatus for polypeptide production according to claim 1, characterized in that, The raw material temporary storage module (1) includes several raw material storage tanks (101) that are separated from each other, and each raw material storage tank (101) is provided with a discharge pipe (2) at the bottom.

3. The fully enclosed synthesis apparatus for polypeptide production according to claim 1, characterized in that, The transfer module (4) includes several AGV transfer trolleys (401), each of which is equipped with a temporary storage tank (5). The top of the temporary storage tank (5) is equipped with a feeding pipe (13). The AGV transfer trolley (401) is also equipped with a discharge pump (14) that communicates with the bottom of the temporary storage tank (5). The outlet end of the discharge pump (14) is also equipped with a conveying pipe (15).

4. The fully enclosed synthesis apparatus for polypeptide production according to claim 3, characterized in that, Each of the AGV transfer trolleys (401) is also equipped with a robotic arm (16), and the robotic arm (16) is equipped with a guide hose (17). The guide hose (17) is connected to the feeding pipe (13) and the conveying pipe (15) respectively. The feeding pipe (13) and the conveying pipe (15) are both equipped with regulating valves (18) for controlling their on / off states.

5. A fully enclosed synthetic apparatus for polypeptide production according to claim 1, characterized in that, The synthesis reaction module (6) includes several reaction vessels (601), and each reaction vessel (601) is connected in series in the order of the reaction process, and each reaction zone is connected in series through a feed pipe (9).

6. A fully enclosed synthesis apparatus for polypeptide production according to claim 4, characterized in that, The synthesis device also includes several barcode scanners (19), and each of the robotic arms (16) of the AGV transfer carts (401) is equipped with an identification code.

7. The fully enclosed synthesis apparatus for polypeptide production according to claim 1, characterized in that, The drying module (7) includes a drying chamber (701), the top of which is provided with a feed inlet connected to the discharge pipe (11); a heating module is also provided inside the drying chamber (701); a condenser (703) is also connected to the top of the drying chamber (701) through an exhaust pipe (702), and a vacuum pump (704) is provided on the exhaust pipe (702).

8. A fully enclosed synthesis apparatus for polypeptide production according to claim 7, characterized in that, The heating module includes several electric heating tubes (705), each heating tube (705) is evenly arranged in the drying oven (701), and each heating tube (705) is also provided with several heat dissipation fins (706) on its surface.

9. A fully enclosed synthesis apparatus for polypeptide production according to claim 8, characterized in that, The drying module (7) further includes a weighing module (20), and the drying chamber (701) is disposed on the weighing module (20); the weighing module (20) is electrically connected to the controller (8).

10. A fully enclosed synthesis apparatus for polypeptide production according to claim 1, characterized in that, The controller (8) includes an industrial control computer and a PLC that are connected in communication.

Images