A portable environmental DNA sampling device
Patent Information
- Application Number
- CN202522063002.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-09-25
- Publication Date
- 2026-09-11
- Estimated Expiration
- 2035-09-25
AI Technical Summary
[0003]然而，现有的环境DNA采样设备在实际使用中仍存在诸多局限性
[0011]与现有技术相比，本实用新型的有益效果是：本便携式环境DNA采样设备，具有以下好处：
Smart Images
Figure CN224741061U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of environmental DNA sampling technology, specifically a portable environmental DNA sampling device. Background Technology
[0002] Environmental DNA (eDNA) technology, as an emerging biomonitoring method, has demonstrated significant advantages in ecological research, species diversity surveys, and environmental assessments in recent years. This technology captures fragments of genetic material released by organisms from environmental media such as water, soil, or air, enabling indirect determination of the presence of specific species or communities. It is non-invasive, highly sensitive, and widely applicable. It not only helps monitor the distribution of rare, endangered, or invasive species but can also be used to assess the health of aquatic ecosystems, providing crucial data support for biodiversity conservation and water environment management. Therefore, developing efficient, reliable, and field-suitable environmental DNA sampling methods is of great significance for promoting the transformation of this technology from scientific research to routine monitoring applications.
[0003] However, existing environmental DNA sampling devices still have many limitations in practical use. Traditional sampling methods mostly rely on manual operation, such as using disposable water samplers or filtration devices, which is cumbersome and prone to introducing exogenous contamination, seriously affecting sample quality and the reliability of subsequent analysis. In addition, most existing devices lack integrated automatic control functions, such as the inability to accurately control sampling flow rate and volume, the inability to achieve simultaneous multi-depth sampling, and the lack of effective monitoring of filter status (such as clogging) during sampling, resulting in low sampling efficiency and poor consistency. To address these issues, we propose a portable environmental DNA sampling device. Summary of the Invention
[0004] The technical problem to be solved by this utility model is to overcome the existing defects and provide a portable environmental DNA sampling device that can realize automated multi-depth sampling, accurately control the sampling process, and effectively monitor the filter status to prevent external contamination, thus effectively solving the problems in the background art.
[0005] To achieve the above objectives, this utility model provides the following technical solution: a portable environmental DNA sampling device, comprising a mounting box and a sampling component; Installation box: The rear side of the interior is provided with a first water tank and a second water tank. The interior of the installation box is provided with two fixed channels, and the inlet hose and the drain hose are fixed in the two fixed channels respectively. The inlet hose is connected to the first water tank. The drain hose is connected to the second water tank. An electromagnetic flow meter is installed on the circumference of the drain hose. The upper side of the installation box is provided with a push-button switch, a power connector and a data connector. The front side of the installation box is provided with a fixed cavity, and the fixed cavity is provided with a control circuit board. The front side of the installation box is provided with a control touch screen. The left side of the front end of the installation box is provided with a water pumping component and a water inlet component. The water pumping component is connected to the first water tank. The right side of the front end of the installation box is provided with a drain component. The drain component is connected to the second water tank. The upper side of the installation box is provided with a protective component. Sampling assembly: includes a sampling chamber, a fixing ring, a support ring, a rubber retaining ring, a capsule filter, an exhaust hose, and a solenoid valve. The sampling chamber is located on the upper side of the mounting box. A fixing ring is fixed inside the sampling chamber, and a support ring is engaged inside the fixing ring. A rubber retaining ring is fixed inside the support ring, and the rubber retaining ring has evenly distributed anti-slip grooves inside. The capsule filter is engaged inside the rubber retaining ring. An exhaust hose is fitted onto the exhaust pipe of the capsule filter. A fixing hole is located on the upper side of the sampling chamber, and the upper end of the exhaust hose is fixed inside the fixing hole. A solenoid valve is installed on the circumference of the exhaust hose. The upper ends of the inlet hose and outlet hose are respectively fitted onto the inlet head and outlet head on the surface of the capsule filter. The sampling assembly is used to sample DNA from the water. Wherein: the input end of the control circuit board is electrically connected to the output end of the push-button switch; the control circuit board is bidirectionally electrically connected to the data connector, the control touch screen, and the electromagnetic flow meter; the input end of the solenoid valve is electrically connected to the output end of the control circuit board; and the output end of the power connector is electrically connected to the input end of the push-button switch.
[0006] Furthermore, the protective assembly includes a protective plate, a limiting post, a slot, a locking plate, a spring, a strip opening, and a pull plate. The upper end of the sampling chamber is provided with a protective plate. A groove is formed on the upper side of the mounting box, and the protective plate is engaged within the groove. A locking hole is formed at the left end of the groove, and a limiting post is engaged within the locking hole. The limiting post is fixed to the lower side of the protective plate. A slot is formed on the right side of the protective plate, and a locking plate is engaged within the slot. A sliding groove is formed on the right side of the groove, and the locking plate is slidably connected within the sliding groove. A spring is fixed to the right side of the locking plate, and the spring is fixed within the sliding groove. A strip opening is formed on the upper side of the sliding groove, and a pull plate is slidably connected within the strip opening. The pull plate is fixed to the upper side of the locking plate. This protective assembly protects the sampling assembly.
[0007] Furthermore, the pumping assembly includes an installation cavity, a water pump, a fixed pipe, an inlet pipe, and a one-way valve. An installation cavity is located on the lower side of the front end of the installation box. A water pump is installed inside the installation cavity. A fixed pipe is fixed inside the water pump's inlet, and an inlet pipe is fixed inside the water pump's outlet. An installation hole is opened on the rear side of the installation cavity, and the inlet pipe is fixed inside the installation hole. The rear end of the inlet pipe is located inside the first water chamber. A one-way valve is installed on the circumferential surface of the inlet pipe. The input end of the water pump is electrically connected to the output end of the control circuit board. The pumping assembly pumps external water into the first water chamber.
[0008] Furthermore, the water inlet assembly includes a five-way solenoid valve, connecting hoses, and water inlet connectors. The five-way solenoid valve is fixed inside the mounting cavity. The right end of the fixed pipe is connected to the outlet port of the five-way solenoid valve. Connecting hoses are fixed inside the four water inlet ports of the five-way solenoid valve. Water inlet connectors are fixed at the front end of the connecting hoses. Four corresponding water inlets are opened at the lower front end of the mounting box. The water inlet connectors are fixed inside the corresponding water inlets. The input end of the five-way solenoid valve is electrically connected to the output end of the control circuit board. By setting four water inlet connectors, users can select an appropriate number of guide hoses to connect to the four water inlet connectors according to their needs. Then, the four barrier mesh buckets on the four guide hoses are placed at different depths of water, so that water samples at different depths can be taken.
[0009] Furthermore, the drainage assembly includes a drainage channel and a drainage connector. A drainage channel is provided at the right end of the front side of the mounting box. The drainage channel is connected to the second water tank. A drainage connector is fixed at the front end inside the drainage channel. Drainage is carried out by setting up the drainage assembly.
[0010] Furthermore, it also includes a flow guiding component, which includes a flow guiding hose and a barrier mesh barrel. The flow guiding hose is sleeved on the surface of the water inlet connector on the left side. The end of the flow guiding hose away from the water inlet connector is fixed inside the barrier mesh barrel. The barrier mesh barrel has evenly distributed strip-shaped openings on its circumference. The barrier mesh barrel is used to block large particles of debris in the water.
[0011] Compared with the prior art, the beneficial effects of this utility model are as follows: This portable environmental DNA sampling device has the following advantages: 1. This utility model achieves automation and precision in the environmental DNA sampling process through highly integrated automatic control and multi-component collaborative work. The built-in capsule filter and electromagnetic flow meter can monitor the sampling flow and filter status in real time, effectively avoiding external contamination introduced by traditional manual operation, and significantly improving the consistency and reliability of the samples. At the same time, the integrated design of the protective components and the convenient sampling structure make filter replacement simple and quick, further ensuring sampling efficiency and the applicability of the equipment in the field environment.
[0012] 2. The equipment supports the flexible configuration of multiple water inlet connectors and flow guiding components to achieve synchronous or selective collection of water samples at different water depths, overcoming the limitations of single-point sampling in existing technologies and providing more comprehensive and reliable technical support for environmental DNA research. Attached Figure Description
[0013] Figure 1 This is a schematic diagram of the front structure of this utility model; Figure 2 This is a front sectional view of the present invention; Figure 3 This utility model Figure 2 Enlarged view of point A in the middle; Figure 4 This is a top sectional view of the present invention; Figure 5 This is a cross-sectional view of the right side of this utility model.
[0014] In the diagram: 1. Installation box; 2. Sampling assembly; 21. Sampling chamber; 22. Fixing ring; 23. Support ring; 24. Rubber retaining ring; 25. Bladder filter; 26. Exhaust hose; 27. Solenoid valve; 3. Protective assembly; 31. Protective plate; 32. Limiting post; 33. Slot; 34. Plate; 35. Spring; 36. Strip opening; 37. Pull plate; 4. Pumping assembly; 41. Installation chamber; 42. Water pump; 43. Fixing pipe; 44. Inlet pipe; 45. Check valve; 5. Inlet assembly; 51. Five-way solenoid valve; 52. Connecting hose; 53. Inlet connector; 6. Drainage assembly; 61. Drainage channel; 62. Drainage connector; 7. Flow guiding assembly; 71. Flow guiding hose; 72. Barrier mesh barrel; 8. First water tank; 9. Second water tank; 10. Inlet hose; 11. Drainage hose; 12. Control circuit board; 13. Control touch screen; 14. Push button switch; 15. Power connector; 16. Data connector; 17. Electromagnetic flow meter. Detailed Implementation
[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.
[0016] Please see Figure 1-5 This embodiment provides a technical solution: a portable environmental DNA sampling device, including a mounting box 1 and a sampling component 2; Installation box 1: The rear side of the interior is equipped with a first water tank 8 and a second water tank 9. The interior of installation box 1 has two fixed channels, each containing a water inlet hose 10 and a water outlet hose 11. The water inlet hose 10 is connected to the first water tank 8. The right side has a fixed bucket, and the water outlet hose 11 is connected to the second water tank 9. An electromagnetic flow meter 17 is installed on the circumference of the water outlet hose 11. The upper side of installation box 1 is equipped with a push-button switch 14, a power connector 15, and a data connector 16. The front side of the interior of installation box 1 has a fixed cavity containing a control circuit board 12. A control touchscreen 13 is also installed on the front side of installation box 1. The left side of the front end of the interior of installation box 1 is equipped with a pumping assembly 4 and a water inlet assembly 5. The pumping assembly 4 is connected to the first water tank 8. A water tank 8 is connected to the first water tank 9. A drainage component 6 is installed on the right side of the front end inside the installation box 1. The drainage component 6 is connected to the second water tank 9. A protective component 3 is installed on the upper side of the installation box 1. The protective component 3 includes a protective plate 31, a limiting post 32, a slot 33, a locking plate 34, a spring 35, a strip-shaped opening 36, and a pull plate 37. A protective plate 31 is provided at the upper end of the sampling chamber 21. A groove is opened on the upper side of the installation box 1. The protective plate 31 is snapped into the inside of the groove. A locking hole is opened at the left end of the groove. The limiting post 32 is snapped into the inside of the locking hole. The limiting post 32 is fixed to the lower side of the protective plate 31. A slot 33 is opened on the right side of the protective plate 31. The locking plate 34 is snapped into the inside of the slot 33. A sliding groove is opened on the right side of the groove. The locking plate 34 is slidably connected to the sliding groove. Inside the mounting box 34, a spring 35 is fixed to the right side of the card plate 34. The spring 35 is fixed inside the slide groove. A strip-shaped opening 36 is opened on the upper side of the slide groove. A pull plate 37 is slidably connected inside the strip-shaped opening 36. The pull plate 37 is fixed to the upper side of the card plate 34. The water pumping assembly 4 includes a mounting cavity 41, a water pump 42, a fixed pipe 43, a water inlet pipe 44, and a one-way valve 45. A mounting cavity 41 is provided on the lower side of the front end of the mounting box 1. A water pump 42 is installed inside the mounting cavity 41. A fixed pipe 43 is fixed inside the water inlet of the water pump 42. A water inlet pipe 44 is fixed inside the water outlet of the water pump 42. A mounting hole is opened on the rear side of the mounting cavity 41. The water inlet pipe 44 is fixed inside the mounting hole. The rear end of the water inlet pipe 44 is located inside the first water tank 8. A one-way valve 45 is installed on the circumferential surface of 44. The input end of the water pump 42 is electrically connected to the output end of the control circuit board 12. The water inlet assembly 5 includes a five-way solenoid valve 51, a connecting hose 52, and a water inlet connector 53. The five-way solenoid valve 51 is fixed inside the mounting cavity 41. The right end of the fixing pipe 43 is connected to the water outlet port of the five-way solenoid valve 51. The connecting hose 52 is fixed inside the four water inlet ports of the five-way solenoid valve 51. The water inlet connector 53 is fixed at the front end of the connecting hose 52. Four corresponding water inlets are opened at the lower front side of the mounting box 1. The water inlet connector 53 is fixed inside the corresponding water inlet. The input end of the five-way solenoid valve 51 is electrically connected to the output end of the control circuit board 12. The drainage assembly 6 includes a drainage channel 61 and a drainage connector 62.A drainage channel 61 is provided at the right end of the front side of the installation box 1. The drainage channel 61 communicates with the second water tank 9. A drainage connector 62 is fixed at the front end of the drainage channel 61. It also includes a flow guiding component 7, which consists of a flow guiding hose 71 and a barrier mesh barrel 72. The flow guiding hose 71 is fitted onto the surface of the water inlet connector 53 on the left side. The end of the flow guiding hose 71 away from the water inlet connector 53 is fixed inside the barrier mesh barrel 72. The barrier mesh barrel 72 has evenly distributed strip-shaped openings on its circumference. The barrier mesh barrel 72 blocks large particles of debris in the water. The drainage component 6 drains the water. Four water inlet connectors 53 allow the user to select the appropriate number of flow guiding hoses 71 to connect to the four inlet connectors 53 as needed. The four barrier mesh barrels 72 on the four flow guiding hoses 71 are then placed at different depths in the water, allowing for water sampling at different depths. A pumping component 4 pumps external water into the first water tank 8. A protective component 3 protects the sampling component 2. Sampling component 2 includes a sampling chamber 21, a fixing ring 22, a support ring 23, a rubber retaining ring 24, a capsule filter 25, an exhaust hose 26, and a solenoid valve 27. The sampling chamber 21 is located on the upper side inside the mounting box 1. The fixing ring 22 is fixed inside the sampling chamber 21. The support ring 23 is snapped into the inside of the fixing ring 22. The rubber retaining ring 24 is fixed inside the support ring 23. The rubber retaining ring 24 has evenly distributed anti-slip grooves inside. The capsule filter 25 is snapped into the inside of the rubber retaining ring 24. The exhaust hose 26 is sleeved on the exhaust pipe of the capsule filter 25. The upper side inside the sampling chamber 21 has a fixing hole. The upper end of the exhaust hose 26 is fixed inside the fixing hole. The solenoid valve 27 is installed on the circumference of the exhaust hose 26. The upper ends of the water inlet hose 10 and the water outlet hose 11 are respectively sleeved on the water inlet head and the water outlet head set on the surface of the capsule filter 25. The sampling component 2 is used to sample DNA in the water. Specifically: the input end of the control circuit board 12 is electrically connected to the output end of the push button switch 14; the control circuit board 12 is bidirectionally electrically connected to the data connector 16, the control touch screen 13, and the electromagnetic flow meter 17; the input end of the solenoid valve 27 is electrically connected to the output end of the control circuit board 12; and the output end of the power connector 15 is electrically connected to the input end of the push button switch 14.
[0017] The working principle of the portable environmental DNA sampling device provided by this utility model is as follows: When the device is running, the user sets parameters, namely sampling flow rate and pressure threshold, through the control touch screen 13, and then starts the program. The control circuit board 12 then coordinates the work of each component: the water pump 42 starts and draws in the water to be tested through the selected water inlet connector 53 and the guide hose 71. During the suction process, the barrier screen 72 at the front end of the guide hose 71 can intercept large particles of impurities. The water flows through the five-way solenoid valve 51 and the fixed pipe 43 and is pumped into the first water chamber 8 for temporary storage. Subsequently, the water flows through the water inlet hose 10 into the capsule filter 25 under pressure, where environmental DNA is efficiently captured. The filtered water flows into the second water chamber 9 through the drain hose 11. Finally, the water flows out of the device through the drain connector 62 of the drain assembly 6. When a blockage occurs, the electromagnetic flow meter 17 installed on the drain hose 11 will show fluctuations in the flow rate and a significant reduction in water volume. This abnormal data is fed back to the control circuit in real time. The circuit board 12 triggers an alarm, and the alarm data will be collected and stored by the control circuit board 12. The user can view the fault details on the historical alarm interface of the control touch screen 13. If a fault occurs during operation, the control circuit board 12 will automatically shut down the water pump 42. Before sampling, the solenoid valve 27 above the capsule filter 25 is opened in a controlled manner to discharge the air inside the filter, and then closes again. After sampling, pull the pull plate 37 to the right to compress the spring 35 of the clamping plate 34 and remove it from the slot 33 of the protective plate 31. Then, move the protective plate 31 horizontally so that the bottom limiting post 32 disengages from the groove of the mounting box 1 to expose the sampling chamber 21. Then, pinch the body of the capsule filter 25 and pull it vertically upward to make it overcome the resistance of the anti-slip groove inside the rubber clamping ring 24 and fall off the rubber clamping ring 24. At the same time, separate the inlet hose 10 and the drain hose 11 from the filter. Then, the capsule filter 25 can be taken out. Then, the DNA inside the capsule filter 25 can be analyzed by external equipment.
[0018] It is worth noting that the control circuit board 12, solenoid valve 27, water pump 42, five-way solenoid valve 51, control touch screen 13 and electromagnetic flow meter 17 disclosed in the above embodiments can be freely configured according to the actual application scenario. The control circuit board 12 controls the operation of solenoid valve 27, water pump 42 and five-way solenoid valve 51 using methods commonly used in the prior art.
[0019] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.
Claims
1. A portable environmental DNA sampling device, characterized by: Includes a mounting box (1) and a sampling component (2); Installation box (1): The rear side of the interior is provided with a first water tank (8) and a second water tank (9). The interior of the installation box (1) is provided with two fixed channels, and the inlet hose (10) and the drain hose (11) are fixed inside the two fixed channels respectively. The inlet hose (10) is connected to the first water tank (8). The drain hose (11) is connected to the second water tank (9). An electromagnetic flow meter (17) is installed on the circumference of the drain hose (11). The upper side of the installation box (1) is provided with a push button switch (14), a power connector (15) and a power supply connector (17). Data connector (16), a fixed cavity is provided on the front side inside the mounting box (1), a control circuit board (12) is installed inside the fixed cavity, a control touch screen (13) is installed on the front side of the mounting box (1), a water pumping assembly (4) and a water inlet assembly (5) are installed on the left side of the front end inside the mounting box (1), the water pumping assembly (4) is connected to the first water tank (8), a drainage assembly (6) is installed on the right side of the front end inside the mounting box (1), the drainage assembly (6) is connected to the second water tank (9), and a protective assembly (3) is installed on the upper side of the mounting box (1). Sampling assembly (2): includes a sampling chamber (21), a fixing ring (22), a support ring (23), a rubber retaining ring (24), a capsule filter (25), an exhaust hose (26), and a solenoid valve (27). The sampling chamber (21) is provided on the upper side inside the mounting box (1). The fixing ring (22) is fixed inside the sampling chamber (21). The support ring (23) is snapped into the inside of the fixing ring (22). The rubber retaining ring (24) is fixed inside the support ring (23). The rubber retaining ring (24) has a uniform opening inside. The anti-slip grooves are evenly distributed. The capsule filter (25) is snapped into the inside of the rubber retainer (24). The exhaust pipe of the capsule filter (25) is fitted with an exhaust hose (26). A fixing hole is opened on the upper side of the sampling chamber (21). The upper end of the exhaust hose (26) is fixed inside the fixing hole. A solenoid valve (27) is installed on the circumferential surface of the exhaust hose (26). The upper ends of the water inlet hose (10) and the water outlet hose (11) are respectively fitted onto the water inlet head and the water outlet head set on the surface of the capsule filter (25). Wherein: the input end of the control circuit board (12) is electrically connected to the output end of the push button switch (14), the control circuit board (12) is bidirectionally electrically connected to the data connector (16), the control touch screen (13) and the electromagnetic flow meter (17), the input end of the solenoid valve (27) is electrically connected to the output end of the control circuit board (12), and the output end of the power connector (15) is electrically connected to the input end of the push button switch (14).
2. The portable environmental DNA sampling device according to claim 1, characterized in that: The protective assembly (3) includes a protective plate (31), a limiting post (32), a slot (33), a locking plate (34), a spring (35), a strip-shaped opening (36), and a pull plate (37). The upper end of the sampling chamber (21) is provided with a protective plate (31). The upper side of the mounting box (1) is provided with a groove. The protective plate (31) is snapped into the inside of the groove. The left end of the groove is provided with a locking hole. The limiting post (32) is snapped into the inside of the locking hole. The limiting post (32) is fixed to the lower side of the protective plate (31). The protective plate (31) has a slot (33) on its right side, and the slot (33) is engaged with a plate (34). The groove has a sliding groove on its right side, and the plate (34) is slidably connected to the inside of the sliding groove. A spring (35) is fixed on the right side of the plate (34), and the spring (35) is fixed inside the sliding groove. A strip-shaped opening (36) is opened on the upper side of the inside of the sliding groove, and a pull plate (37) is slidably connected inside the strip-shaped opening (36). The pull plate (37) is fixed on the upper side of the plate (34).
3. The portable environmental DNA sampling device according to claim 1, characterized in that: The pumping assembly (4) includes a mounting cavity (41), a water pump (42), a fixed pipe (43), an inlet pipe (44), and a one-way valve (45). The mounting cavity (41) is provided on the lower side of the front end of the mounting box (1). The water pump (42) is installed inside the mounting cavity (41). The fixed pipe (43) is fixed inside the inlet of the water pump (42). The inlet pipe (44) is fixed inside the outlet of the water pump (42). The mounting hole is opened on the rear side of the mounting cavity (41). The inlet pipe (44) is fixed inside the mounting hole. The rear end of the inlet pipe (44) is located inside the first water chamber (8). The one-way valve (45) is installed on the circumferential surface of the inlet pipe (44). The input end of the water pump (42) is electrically connected to the output end of the control circuit board (12).
4. A portable environmental DNA sampling device according to claim 3, characterized in that: The water inlet assembly (5) includes a five-way solenoid valve (51), a connecting hose (52), and a water inlet connector (53). The five-way solenoid valve (51) is fixed inside the mounting cavity (41). The right end of the fixed pipe (43) is connected to the water outlet port of the five-way solenoid valve (51). The connecting hose (52) is fixed inside the four water inlet ports of the five-way solenoid valve (51). The water inlet connector (53) is fixed at the front end of the connecting hose (52). Four corresponding water inlets are opened at the lower front end of the mounting box (1). The water inlet connector (53) is fixed inside the corresponding water inlet. The input end of the five-way solenoid valve (51) is electrically connected to the output end of the control circuit board (12).
5. A portable environmental DNA sampling device according to claim 1, characterized in that: The drainage assembly (6) includes a drainage channel (61) and a drainage connector (62). The right end of the front side of the mounting box (1) is provided with a drainage channel (61). The drainage channel (61) is connected to the second water tank (9). The front end of the drainage channel (61) is fixed with a drainage connector (62).
6. A portable environmental DNA sampling device according to claim 4, characterized in that: It also includes a flow guiding component (7), which includes a flow guiding hose (71) and a barrier mesh barrel (72). The flow guiding hose (71) is sleeved on the surface of the water inlet connector (53) on the left side. The end of the flow guiding hose (71) away from the water inlet connector (53) is fixed inside the barrier mesh barrel (72). The barrier mesh barrel (72) has evenly distributed strip-shaped openings on its circumferential surface.