A novel fusion enzyme concentration device

Abstract

The utility model relates to the field of bioengineering technology discloses a kind of concentration device of novel fusion enzyme, including reaction kettle, the outside fixed connection of reaction kettle has control mechanism, the inside rotary connection of reaction kettle has stirring mechanism, the top fixed connection of reaction kettle has ration mechanism, the outside fixed connection of reaction kettle has discharge gate, the control mechanism includes water inlet pipe, the inside fixed connection of water inlet pipe in the inside of reaction kettle, the inside fixed connection of water inlet pipe has two limit plates, the inside sliding connection of limit plate has baffle, the bottom fixed connection of baffle has support assembly.In the utility model, fusion enzyme flows into reaction kettle through water inlet pipe, baffle does not block water flow in initial state, with liquid level rising, liquid enters through the water inlet hole of protection cover, float rises by buoyancy, baffle is pushed along limit plate and moves up by support rod.

Description

Technical Field

[0001] This invention relates to the field of bioengineering technology, and in particular to a novel device for concentrating fusion enzymes. Background Technology

[0002] Fusion enzymes are enzymes with special functions constructed through biotechnology such as genetic engineering. Their concentration process is an important step in the post-processing of biological products, involving the separation and purification of biomolecules. The aim is to improve the concentration and purity of fusion enzymes to meet the needs of subsequent applications, such as medical and industrial production.

[0003] By employing processes adapted to the characteristics of enzyme proteins, such as ultrafiltration, membrane separation, vacuum concentration, or centrifugation, the fusion enzyme can be efficiently separated from moisture and small molecule impurities in the reaction system under mild conditions, thereby improving the concentration and purity of the fusion enzyme. This device not only reduces the storage volume of enzyme preparations and lowers transportation costs, but also removes impurities that affect enzyme activity and enhances its stability. It provides key post-processing technology support for the large-scale application of fusion enzymes in biopharmaceuticals, industrial catalysis, food processing, and other fields, ensuring the efficient utilization and performance of enzymes in downstream production.

[0004] In existing technologies, for precision bioprocesses such as the concentration of novel fusion enzymes, uncontrolled water volume can disrupt the concentration balance and pH stability of the reaction system, leading to denaturation, inactivation, or insufficient purity of the fusion enzyme, resulting in the scrapping of batches of products. To address this issue, a novel fusion enzyme concentration device is proposed. Utility Model Content

[0005] To overcome the above deficiencies, this invention provides a novel fusion enzyme concentration device, which aims to improve the problem in the prior art where uncontrolled water volume can disrupt the concentration balance and pH stability of the reaction system, leading to denaturation, inactivation, or insufficient purity of the fusion enzyme.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A novel fusion enzyme concentration device includes a reaction vessel, a control mechanism fixedly connected to the outside of the reaction vessel, a stirring mechanism rotatably connected inside the reaction vessel, a metering mechanism fixedly connected to the top of the reaction vessel, and a discharge port fixedly connected to the outside of the reaction vessel.

[0008] The control mechanism includes a water inlet pipe, which is externally and fixedly connected to the inside of the reactor. Two limiting plates are fixedly connected to the inside of the water inlet pipe, and a baffle is slidably connected to the inside of the limiting plates. A support assembly is fixedly connected to the bottom of the baffle, and a protective cover is fixedly connected to the bottom of the water inlet pipe. A fixing plate is fixedly connected to the inside of the protective cover.

[0009] As a further description of the above technical solution:

[0010] The metering mechanism includes multiple fixed blocks, the bottom of each fixed block is fixedly connected to the top of the reactor, two fixed blocks are fixedly connected to a pneumatic cylinder, the driving end of the pneumatic cylinder is fixedly connected to a piston, the piston is slidably connected to a connecting pipe, the other end of the connecting pipe is fixedly connected to a connecting pipe, and the connecting pipe is slidably connected to a fixing ring.

[0011] As a further description of the above technical solution:

[0012] The fixed ring has multiple limiting grooves inside, a sliding column is slidably connected inside the fixed ring, a connecting rod is fixedly connected to the top of the sliding column, and a sliding plate is fixedly connected to the top of the connecting rod.

[0013] As a further description of the above technical solution:

[0014] The four ends of the sliding plate are slidably connected to the inside of the limiting groove, and a storage bin is fixedly connected to the top of the connecting pipe.

[0015] As a further description of the above technical solution:

[0016] The stirring mechanism includes a motor, the bottom of which is fixedly connected to the top of the reaction vessel, a rotating rod is fixedly connected to the drive end of the motor, and multiple rotating plates are fixedly connected to the outside of the rotating rod.

[0017] As a further description of the above technical solution:

[0018] The bottom of the reactor is fixedly connected to a support frame, the outside of the rotating plate is slidably connected to the inside of the reactor, and the outside of the rotating rod is rotatably connected to the inside of the reactor.

[0019] As a further description of the above technical solution:

[0020] The support assembly includes a spring, the top of which is fixedly connected to the bottom of the baffle, the bottom of which is fixedly connected to the top of the fixed plate, a support rod fixedly connected to the bottom of the baffle, and a float fixedly connected to the bottom of the support rod.

[0021] As a further description of the above technical solution:

[0022] The bottom of the protective cover has two water inlet holes, and the outside of the float is slidably connected to the inside of the protective cover.

[0023] This utility model has the following beneficial effects:

[0024] 1. In this utility model, the fusion enzyme flows into the reaction vessel through the water inlet pipe. In the initial state, the baffle does not block the water flow. As the liquid level rises, the liquid enters through the water inlet hole of the protective cover. The float rises due to buoyancy and pushes the baffle to move up the limiting plate through the support rod. When the liquid level reaches the preset height, the water inlet pipe is blocked to stop the water inlet. When the liquid level drops, the spring pulls the baffle to reset and resume the water inlet.

[0025] 2. In this utility model, the starting pneumatic cylinder drives the piston to move inside the connecting pipe. The change in air pressure causes the solution in the storage bin to be transported through the connecting pipe. The slide plate slides along the limiting groove of the fixed ring, driving the slide column to precisely control the solution flow rate. At the same time, the motor drives the rotating rod to rotate the rotating plate, so that the materials are fully mixed. After concentration, the product is discharged from the outlet. The support frame ensures the stable operation of the device. Attached Figure Description

[0026] Figure 1 This is a three-dimensional schematic diagram of a novel fusion enzyme concentration device proposed in this utility model;

[0027] Figure 2 This is a schematic diagram of the reaction vessel of a novel fusion enzyme concentration device proposed in this utility model;

[0028] Figure 3 for Figure 2 Enlarged view of point A in the middle;

[0029] Figure 4 for Figure 2 Enlarged view of point B in the middle.

[0030] Legend:

[0031] 1. Reactor; 2. Control mechanism;

[0032] 21. Water inlet pipe; 22. Limiting plate; 23. Baffle; 24. Protective cover; 25. Fixing plate;

[0033] 26. Support assembly; 261. Spring; 262. Support rod; 263. Float;

[0034] 27. Water inlet hole;

[0035] 3. Measuring mechanism; 31. Fixing block; 32. Pneumatic cylinder; 33. Piston; 34. Connecting pipe; 35. Connecting pipe; 36. Fixing ring; 37. Restricting groove; 38. Slide plate; 39. Connecting rod; 310. Sliding column; 311. Storage bin;

[0036] 4. Stirring mechanism; 41. Motor; 42. Rotating rod; 43. Rotating plate;

[0037] 5. Discharge port; 6. Support frame. Detailed Implementation

[0038] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0039] Example:

[0040] A novel fusion enzyme concentration device, referring to Figures 1 to 3 This invention includes a novel fusion enzyme concentration device, comprising a reaction vessel 1. An automatic water inlet control mechanism 2 is fixedly connected to the outside of the reaction vessel 1, effectively preventing excessive water in the reaction vessel 1 from affecting the concentration effect. An internal stirring mechanism 4 is rotatably connected to the reaction vessel 1 to mix the materials, ensuring full contact between the fusion enzyme and the added solution and improving concentration efficiency. A quantitative mechanism 3 is fixedly connected to the top of the reaction vessel 1 to precisely control the amount of added solution, ensuring accurate proportions of the added mixed solution. An outlet 5 is fixedly connected to the outside of the reaction vessel 1 for discharging the concentrated product. The control mechanism 2 includes a water inlet pipe 21 for conveying the fusion enzyme. The external fixed connection is inside the reactor 1 to ensure that the fusion enzyme can smoothly enter the reactor 1. The water inlet pipe 21 is fixedly connected to two limiting plates 22 to restrict the movement direction of the baffle 23, so that the baffle 23 can only slide up and down. The limiting plates 22 are slidably connected to a baffle 23 that can block the water flow. The water inlet is opened and closed by the raising and lowering of the baffle 23. The bottom of the baffle 23 is fixedly connected to a support assembly 26 that can drive the baffle 23 to rise and fall, providing power for the movement of the baffle 23. The bottom of the water inlet pipe 21 is fixedly connected to a protective cover 24 to protect the internal components and prevent impurities from interfering with the operation of the components. The protective cover 24 is fixedly connected to a fixing plate 25 to support the spring 261.

[0041] Specifically, the operation of the control mechanism 2 relies on the synergistic effect of buoyancy and mechanical vibration. When the fusion enzyme flows into the reactor 1 through the water inlet pipe 21, the spring 261 is in a naturally extended state in the initial state, and the baffle 23 is located at the bottom of the limiting plate 22, which does not affect the flow of water. As the liquid level in the reactor 1 rises, the liquid enters the interior of the protective cover 24 through the water inlet hole 27 at the bottom of the protective cover 24. The float 263 floats upward under the action of buoyancy and pushes the baffle 23 to slide upward along the inner side of the limiting plate 22 through the support rod 262. When the liquid level reaches the preset height, the baffle 23 completely blocks the channel of the water inlet pipe 21 and automatically stops the water intake. When the liquid level drops, the float 263 sinks down, and the tension of the spring 261 drives the baffle 23 to reset, restoring the smooth flow of the water inlet channel. This achieves precise control of the amount of material in the reactor 1 and avoids affecting the concentration effect due to excessive feeding. The fixed plate 25 provides stable support for the spring 261, and the protective cover 24 effectively isolates impurities in the material, ensuring the sensitive operation of the support component 26.

[0042] The support assembly 26 includes a spring 261 that can reset the baffle 23. When the water level drops, it drives the baffle 23 to move down to restore water intake. The top of the spring 261 is fixedly connected to the bottom of the baffle 23, and the bottom of the spring 261 is fixedly connected to the top of the fixed plate 25 to provide support for the spring 261. The bottom of the baffle 23 is fixedly connected to a support rod 262 that can transmit buoyancy, which transmits the buoyancy of the float 263 to the baffle 23. The bottom of the support rod 262 is fixedly connected to a float 263 that can generate buoyancy in the water, which moves up and down by changes in water level.

[0043] Specifically, the spring 261 of the support component 26 is located between the baffle 23 and the fixed plate 25. In the initial state, it is in a naturally extended state, providing downward tension to the baffle 23. When the float 263 does not receive sufficient buoyancy, it can keep the baffle 23 in a low position without affecting water intake. The support rod 262 vertically connects the baffle 23 and the float 263, forming a force transmission path. When the water level in the reactor 1 rises, the liquid enters the protective cover 24 through the water inlet 27. The float 263 moves upward under buoyancy and transmits the force to the baffle 23 through the support rod 262, causing it to overcome the tension of the spring 261 and rise. When the water level drops, the buoyancy decreases, and the rebound force of the spring 261 pulls the baffle 23 downward, causing the support rod 262 and the float 263 to reset, realizing the automatic switching of the water intake state.

[0044] The bottom of the protective cover 24 has two water inlet holes 27 that allow liquid to enter, so that liquid can enter the protective cover 24 and generate buoyancy for the float 263. The float 263 is externally slidably connected to the inside of the protective cover 24 to ensure that the float 263 rises and falls stably.

[0045] Specifically, the two water inlet holes 27 at the bottom of the protective cover 24 are symmetrically distributed, which allows the liquid in the reactor 1 to enter the interior of the protective cover 24 evenly, so that the float 263 is balanced on both sides. The outer wall of the float 263 is closely attached to the inner wall of the protective cover 24 and slides smoothly, which ensures that the liquid can flow through the gaps while limiting the float 263 from shifting, and ensures that it rises and falls steadily with the liquid level.

[0046] Reference Figure 2 and Figure 4 The metering mechanism 3 includes multiple fixing blocks 31 for fixing the pneumatic cylinder 32, which enhances the stability of the pneumatic cylinder 32 during operation. The bottom of each fixing block 31 is fixedly connected to the top of the reactor 1, so that the metering mechanism 3 is stably installed on the reactor 1. The pneumatic cylinder 32 that provides power is fixedly connected inside the two fixing blocks 31, which drives the piston 33 to move. The driving end of the pneumatic cylinder 32 is fixedly connected to the piston 33 that can change the air pressure in the connecting pipe 34, and the flow of the solution is controlled by the change of air pressure. The piston 33 is slidably connected to the connecting pipe 34 for conveying the solution, providing a channel for the flow of the solution. The other end of the connecting pipe 34 is fixedly connected to the connecting pipe 35 for docking with the storage bin 311, so as to realize the smooth transfer of the solution. The connecting pipe 35 is slidably connected to the fixing ring 36 for restricting the flow of the solution.

[0047] Specifically, the fixing blocks 31 of the metering mechanism 3 are symmetrically distributed on both sides of the pressure cylinder 32. By fixing the bottom to the reaction vessel 1, they provide stable support for the entire mechanism. When the pressure cylinder 32 is working, it drives the piston 33 to slide back and forth in the connecting pipe 34, changing the air pressure in the pipe. When the piston 33 retracts, it generates negative pressure. The solution in the storage bin 311 is sucked into the connecting pipe 34 through the connecting pipe 35. The fixing ring 36 forms a sealing structure in the connecting pipe 35. With the change of air pressure, the amount of solution sucked in is precisely controlled to ensure that the amount of solution added each time is consistent and to improve the stability of the concentration reaction.

[0048] The fixed ring 36 has multiple limiting grooves 37 inside to restrict the movement of the slide plate 38, ensuring that the slide plate 38 slides along a predetermined trajectory. The fixed ring 36 is slidably connected to a sliding column 310 that can control the flow of solution. The flow rate of solution is adjusted by moving the sliding column 310. The top of the sliding column 310 is fixedly connected to a connecting rod 39 for connecting the slide plate 38 and the sliding column 310, so that the movement of the slide plate 38 can be transmitted to the sliding column 310. The top of the connecting rod 39 is fixedly connected to a slide plate 38 that can slide in the limiting grooves 37, driving the sliding column 310 to move synchronously.

[0049] Specifically, the limiting grooves 37 inside the fixed ring 36 are symmetrically distributed, providing a precise sliding trajectory for the slide plate 38 and preventing it from deviating. The slide plate 38 is rigidly connected to the sliding column 310 through the connecting rod 39, forming a linkage structure. When the pneumatic cylinder 32 drives the piston 33 to move, the change in air pressure in the connecting pipe 34 will push the slide plate 38 to slide along the limiting grooves 37. The connecting rod 39 drives the sliding column 310 to move synchronously within the fixed ring 36. The rise and fall of the sliding column 310 can change the channel size in the connecting pipe 35, thereby precisely controlling the flow rate and on / off of the solution and ensuring the accuracy of the addition amount.

[0050] The four ends of the slide plate 38 are slidably connected to the inside of the limiting groove 37 to ensure the stable sliding of the slide plate 38. The top of the connecting pipe 35 is fixedly connected to a storage bin 311 for storing the mixed solution, providing the solution required for the concentration reaction.

[0051] Specifically, the four ends of the slide plate 38 are embedded in the limiting groove 37 to form a sliding fit, ensuring that it does not deviate during movement and improving the stability of the action. The top of the connecting pipe 35 is connected to the storage bin 311, which can pre-store a sufficient amount of mixed solution and accurately deliver it to the reaction vessel 1 through the connecting pipe 35 to meet the solution supply requirements of the concentrated reaction.

[0052] Reference Figure 1 and Figure 2 The stirring mechanism 4 includes a motor 41 that provides stirring power and drives the stirring action. The bottom of the motor 41 is fixedly connected to the top of the reactor 1 to ensure that the motor 41 is securely installed. The drive end of the motor 41 is fixedly connected to a rotating rod 42 that can drive the rotating plate 43 to rotate, transmitting the power of the motor 41. Multiple rotating plates 43 that can stir materials are fixedly connected to the outside of the rotating rod 42, so that the materials are mixed evenly by rotation.

[0053] Specifically, the motor 41 of the stirring mechanism 4 is fixed to the top of the reactor 1. After starting, it drives the rotating rod 42 to rotate. The rotating rod 42 drives multiple external rotating plates 43 to rotate synchronously. The rotating plates 43 stir the material in the reactor 1, so that the fusion enzyme and the added solution can fully contact and mix, thereby improving the efficiency of the concentration reaction.

[0054] The bottom of the reactor 1 is fixedly connected to a support frame 6 for supporting the device, so that the device is placed stably. The outside of the rotating plate 43 is slidably connected to the inside of the reactor 1, ensuring that the rotating plate 43 can rotate smoothly inside the reactor 1. The outside of the rotating rod 42 is rotatably connected to the inside of the reactor 1, ensuring that the rotating rod 42 rotates stably.

[0055] Specifically, the support frame 6 at the bottom of the reactor 1 is symmetrically distributed, providing stable support for the entire device and preventing shaking during operation. The edge of the rotating plate 43 maintains a small gap with the inner wall of the reactor 1 and slides smoothly, ensuring that there is no jamming during rotation. The rotating rod 42 is connected to the reactor 1 through a bearing, ensuring that it rotates stably under the drive of the motor 41 and providing reliable power transmission for stirring.

[0056] The implementation principle of this application embodiment is as follows: the fusion enzyme is transported through the water inlet pipe 21 and flows into the reaction vessel 1 through the bottom of the limiting plate 22. As the liquid level in the reaction vessel 1 rises, the liquid enters the protective cover 24 through the water inlet hole 27 at the bottom of the protective cover 24. The float 263 rises under the action of buoyancy and pushes the baffle 23 to slide upward along the limiting plate 22 through the support rod 262. When the liquid level reaches the preset height, the baffle 23 completely blocks the water inlet pipe 21, stops the water intake, and avoids over-intake. When the liquid level drops, the spring 261 pulls the baffle 23 to reset and resumes the water intake.

[0057] When a mixed solution needs to be added during the concentration stage, the pneumatic cylinder 32 is activated to drive the piston 33 to move within the connecting pipe 34, causing the solution in the storage bin 311 to fall through the connecting pipe 35. At this time, the sliding column 310 moves the connecting rod 39 and the sliding plate 38 along the limiting groove 37 of the fixed ring 36 as the air pressure changes. The solution flow rate is precisely controlled by the opening and closing of the sliding column 310. At the same time, the motor 41 drives the rotating rod 42 to rotate the rotating plate 43, so that the fusion enzyme and the added solution are fully mixed, improving the concentration efficiency. The final product is discharged through the outlet 5.

[0058] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A novel fusion enzyme concentration device, comprising a reaction vessel (1), characterized in that: The reactor (1) is fixedly connected to a control mechanism (2) on the outside, a stirring mechanism (4) is rotatably connected inside the reactor (1), a metering mechanism (3) is fixedly connected to the top of the reactor (1), and a discharge port (5) is fixedly connected to the outside of the reactor (1). The control mechanism (2) includes a water inlet pipe (21), which is externally fixedly connected to the inside of the reactor (1). Two limiting plates (22) are fixedly connected inside the water inlet pipe (21). A baffle (23) is slidably connected inside the limiting plate (22). A support assembly (26) is fixedly connected to the bottom of the baffle (23). A protective cover (24) is fixedly connected to the bottom of the water inlet pipe (21). A fixing plate (25) is fixedly connected inside the protective cover (24).

2. The novel fusion enzyme concentration device according to claim 1, characterized in that: The quantitative mechanism (3) includes multiple fixed blocks (31), the bottom of each fixed block (31) is fixedly connected to the top of the reactor (1), and two fixed blocks (31) are fixedly connected to a pneumatic cylinder (32). The driving end of the pneumatic cylinder (32) is fixedly connected to a piston (33), and the outside of the piston (33) is slidably connected to a connecting pipe (34). The other end of the connecting pipe (34) is fixedly connected to a connecting pipe (35), and the inside of the connecting pipe (35) is slidably connected to a fixing ring (36).

3. The novel fusion enzyme concentration device according to claim 2, characterized in that: The fixed ring (36) has multiple limiting grooves (37) inside. A sliding column (310) is slidably connected inside the fixed ring (36). A connecting rod (39) is fixedly connected to the top of the sliding column (310). A sliding plate (38) is fixedly connected to the top of the connecting rod (39).

4. The novel fusion enzyme concentration device according to claim 3, characterized in that: The four ends of the sliding plate (38) are evenly connected to the inside of the limiting groove (37), and the top of the connecting pipe (35) is fixedly connected to the storage bin (311).

5. The novel fusion enzyme concentration device according to claim 1, characterized in that: The stirring mechanism (4) includes a motor (41), the bottom of which is fixedly connected to the top of the reactor (1), and a rotating rod (42) is fixedly connected to the drive end of the motor (41). Multiple rotating plates (43) are fixedly connected to the outside of the rotating rod (42).

6. The novel fusion enzyme concentration device according to claim 5, characterized in that: The bottom of the reactor (1) is fixedly connected to a support frame (6), the outside of the rotating plate (43) is slidably connected to the inside of the reactor (1), and the outside of the rotating rod (42) is rotatably connected to the inside of the reactor (1).

7. The novel fusion enzyme concentration device according to claim 1, characterized in that: The support assembly (26) includes a spring (261), the top of which is fixedly connected to the bottom of the baffle (23), the bottom of which is fixedly connected to the top of the fixed plate (25), a support rod (262) is fixedly connected to the bottom of the baffle (23), and a float (263) is fixedly connected to the bottom of the support rod (262).

8. The novel fusion enzyme concentration device according to claim 7, characterized in that: The bottom of the protective cover (24) has two water inlet holes (27), and the float (263) is slidably connected to the inside of the protective cover (24).

Images