A cell culture plate

Abstract

The utility model relates to cell culture device technical field, concretely disclose a cell culture plate, including multi -well plate and protection frame, the upper surface rectangle array of multi -well plate has a plurality of culture holes, the edge of multi -well plate is equipped with the first flanging of downward, the upper portion transverse convex of the periphery of first flanging sets up a first flange, the outer edge of first flange is equipped with the second flanging of downward, the top surface of protection frame is equipped with the recess of corresponding with own shape, the bottom longitudinal convex of recess sets up a second flange, the second flange and the inner edge between protection frame form the liquid storage groove, the second flange and the outer edge between protection frame form the containing groove that can contain second flange, the utility model provides a cell culture plate, can effectively solve the edge hole effect, still can prevent the filling liquid of multi -well plate removal and transportation process spatter, protect experimental environment.

Description

Technical Field

[0001] This utility model relates to the field of cell culture device technology, and in particular to a cell culture plate. Background Technology

[0002] Cell culture plates are used for in vitro cell culture. For these plates, the edge well effect is a significant problem. As the culture medium evaporates, the concentration of culture medium components and drugs within the wells increases, potentially leading to sedimentation and increasing data variability between wells. Many researchers try to improve data reproducibility by avoiding edge wells, but this wastes up to 38% of the culture wells; for example, in a 96-well plate, the outer 36 wells are discarded. Therefore, experimental groups must be distributed across multiple culture plates to achieve sufficient throughput, increasing workload and cost, and reducing the flexibility of culture plate layout in experiments.

[0003] To address the edge hole effect, porous plates with grooved edges have appeared on the market, such as... Figure 1 As shown, a groove is designed at the edge of the multi-well plate. During the experiment, sterile water or sterile PBS is filled into the groove. The filling solution forms a "humidity buffer zone," preventing moisture from evaporating from the edge wells to the outside, reducing heat transfer between the edge wells and the outside environment, and making the temperature of the edge wells more consistent with that of the center wells. Multi-well plates with grooved edges effectively solve the edge well effect, but researchers face inconvenience in use. Because the grooves at the edge of the multi-well plate are filled with liquid, the filling solution in the grooves is easily spilled when the multi-well plate is moved, contaminating the experimental environment. Utility Model Content

[0004] To address the problems mentioned in the background art, the purpose of this utility model is to provide a cell culture plate.

[0005] To achieve the above objectives, the technical solution of this utility model is as follows:

[0006] A cell culture plate includes a multi-well plate and a protective frame. The upper surface of the multi-well plate has a rectangular array of culture wells. The edge of the multi-well plate has a downward-facing first flange. The upper part of the outer periphery of the first flange has a first convex edge protruding laterally. The outer edge of the first convex edge has a downward-facing second flange. The height of the protective frame is greater than the height of the second flange. The inner circle size of the protective frame is adapted to the outer periphery size of the first flange. The top surface of the protective frame has a groove corresponding to its own shape. The bottom of the groove has a second convex edge protruding longitudinally. The height of the second convex edge is less than the depth of the groove. A liquid storage groove is formed between the second convex edge and the inner edge of the protective frame. A receiving groove is formed between the second convex edge and the outer edge of the protective frame to accommodate the second flange. The protective frame is fitted over the first flange, and the second flange is disposed in the receiving groove. The second flange, the second convex edge, and the outer edge of the protective frame are all spaced apart.

[0007] Furthermore, the cell culture plate also includes a cover plate, the edge of which is provided with a downward-facing third flange, the inner circumference of which is adapted to the outer circumference of the second flange, and a third convex edge is provided in the middle of the inner circumference of the third flange.

[0008] The beneficial effects of this invention are as follows: the grooves on the edge of the porous plate are eliminated, and a protective frame is used instead. The liquid storage tank inside the protective frame can hold the filling liquid, and the second flange, the second convex edge, and the outer edge of the protective frame form a maze structure. After the filling liquid evaporates, it can still pass through the maze to form a humidity buffer zone, avoiding the "edge hole effect". Furthermore, when moving the protective frame and the porous plate, the maze structure can also effectively prevent the filling liquid from spilling out of the protective frame, thus avoiding contamination of the experimental environment. Attached Figure Description

[0009] Figure 1 This is a schematic diagram of the background technology;

[0010] Figure 2 This is a perspective view of an embodiment of the present utility model;

[0011] Figure 3 This is an exploded view of an embodiment of the present utility model;

[0012] Figure 4 This is a cross-sectional view of an embodiment of the present utility model.

[0013] Explanation of the attached figures: 1. Multiwell plate; 11. Culture well; 12. First flange; 121. First convex edge; 122. Second flange; 2. Protective frame; 21. Second convex edge; 22. Storage tank; 23. Container tank; 3. Cover plate; 31. Third flange; 311. Third convex edge. Detailed Implementation

[0014] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model are within the protection scope of the present utility model.

[0015] like Figures 2-4 As shown, the cell culture plate includes a multi-well plate 1 and a protective frame 2. The multi-well plate 1 is rectangular, and its upper surface has a rectangular array of 96 culture wells 11. A protective ring protrudes from the top of each culture well 11. The edge of the multi-well plate 1 has a downward-facing first flange 12. A first raised edge 121 protrudes laterally from the upper part of the outer periphery of the first flange 12. A downward-facing second flange 122 is located at the outer edge of the first raised edge 121. The height of the protective frame 2 is greater than the height of the second flange 122. The protective frame 2 is rectangular, and its inner ring size matches the outer periphery size of the first flange 12. The top surface of the protective frame 2 has an opening that conforms to its own shape. The corresponding groove has a second protruding edge 21 extending longitudinally from the bottom of the groove. The height of the second protruding edge 21 is lower than the depth of the groove. A liquid storage tank 22 is formed between the second protruding edge 21 and the inner edge of the protective frame 2. A receiving groove 23 is formed between the second protruding edge 21 and the outer edge of the protective frame 2. The receiving groove 23 can accommodate the second flange 122. The protective frame 2 is fitted over the first flange 12. The inner edge of the protective frame 2 abuts against the first protruding edge 121. The second flange 122 is located in the receiving groove 23. The bottom of the second flange 122 is located below the top of the second protruding edge 21. The second flange 122, the second protruding edge 21, and the outer edge of the protective frame 2 are all spaced apart.

[0016] In actual use, sterile water is first filled into the storage tank 22, then the multi-well plate 1 is connected to the protective frame 2, and then cell culture medium is filled into the culture well 11. The second flange 122, the second convex edge 21 and the outer edge of the protective frame 2 form a maze structure. After the sterile water evaporates, it can still form a humidity buffer zone through the maze, avoiding the "edge hole effect" of the multi-well plate 1. In addition, when the protective frame 2 and the multi-well plate 1 are moved, the maze structure can also effectively prevent the filling liquid from spilling out of the protective frame 2, avoiding contamination of the experimental environment.

[0017] In this embodiment, the cell culture plate also includes a cover plate 3. The edge of the cover plate 3 is provided with a downward-facing third flange 31. The inner circumference of the third flange 31 is adapted to the outer circumference of the second flange 122. A third convex edge 311 is provided in the middle of the inner circumference of the third flange 31. Covering the top of the multi-well plate 1 with the cover plate 3 can protect the cell culture medium.

[0018] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A cell culture plate, comprising a multi-well plate (1), wherein the upper surface of the multi-well plate (1) has a plurality of culture wells (11) arranged in a rectangular array, and the edge of the multi-well plate (1) is provided with a downward-facing first flange (12), characterized in that, The cell culture plate also includes a protective frame (2). A first raised edge (121) protrudes laterally from the upper part of the outer periphery of the first flange (12). A downward-facing second flange (122) is provided on the outer edge of the first raised edge (121). The height of the protective frame (2) is greater than the height of the second flange (122). The inner dimension of the protective frame (2) is adapted to the outer periphery of the first flange (12). A groove corresponding to the shape of the protective frame (2) is opened on its top surface. A second raised edge (21) protrudes longitudinally from the bottom of the groove. The height of the second protruding edge (21) is lower than the depth of the groove. A liquid storage groove (22) is formed between the second protruding edge (21) and the inner edge of the protective frame (2). A receiving groove (23) that can accommodate the second flange (122) is formed between the second protruding edge (21) and the outer edge of the protective frame (2). The protective frame (2) is fitted over the outside of the first flange (12). The second flange (122) is located in the receiving groove (23). The second flange (122) is spaced apart from the second protruding edge (21) and the outer edge of the protective frame (2).

2. The cell culture plate according to claim 1, characterized in that, The cell culture plate also includes a cover plate (3), the edge of which is provided with a downward-facing third flange (31), the inner circumference of which is adapted to the outer circumference of the second flange (122), and a third convex edge (311) is provided in the middle of the inner circumference of the third flange (31).

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