LIVER-PROTECTIVE COMPOSITION
A liver-protective composition using Lysimachia christinae, Aster tataricus, turmeric, ginseng, and Hovenia dulcis Thunb. addresses the need for effective liver treatments by reducing liver damage markers in mice models, offering hepatoprotection and convenience in preparation and use.
Patent Information
- Application Number
- DE102024123537
- Authority / Receiving Office
- DE · DE
- Patent Type
- Applications
- Current Assignee / Owner
- Filing Date
- 2024-08-19
- Publication Date
- 2026-02-19
AI Technical Summary
There is a lack of effective medications for treating or protecting the liver, particularly due to the increasing prevalence of liver diseases caused by viral infections, parasitic infections, alcoholism, and hepatitis in various regions, and existing treatments lack sufficient efficacy and safety.
A liver-protective composition comprising Lysimachia christinae, Aster tataricus, turmeric, ginseng, Hovenia dulcis Thunb., and a spice additive, prepared through grinding, boiling, and extraction processes, resulting in an aqueous or tincture form for oral or topical administration.
The composition effectively protects the liver by reducing liver damage markers (ALT and AST) in mice models, demonstrating significant hepatoprotective effects without notable side effects, and is convenient to prepare and administer.
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Abstract
Description
TECHNICAL AREA
[0001] The present invention relates to the technical field of traditional Chinese medicine and in particular to a liver-protecting composition and a method for its production. BACKGROUND
[0002] The liver is a metabolic organ with diverse functions. It can detoxify various metabolic products, regulate glycogen storage, synthesize plasma proteins, produce hormones, and manufacture biochemical substances that facilitate the breakdown of toxins. Patients with liver disease may experience problems with digestion, blood clotting, blood sugar levels, immunity, lipid metabolism, and more. Fat malabsorption can cause symptoms such as indigestion, deficiencies in fat-soluble vitamins, gallstones, intolerance to fatty foods, alcohol intolerance, bloating, and nausea.
[0003] Regarding the causes of liver disease, viral and parasitic infections are the main causes in Africa and Asia. In Europe and the United States, however, alcoholism is the primary cause of liver disease, while the number of hepatitis virus infections is steadily increasing.
[0004] Although an increasing number of patients suffer from liver diseases, there is currently a lack of medications to treat or protect the liver. Therefore, medical professionals are eagerly awaiting research into medications for the effective treatment or protection of the liver.
[0005] Against this background, the present invention is proposed. SUMMARY
[0006] The first objective of the present invention relates to a liver-protective composition, including in parts by weight: 10 to 25 parts Lysimachia christinae, 5 to 15 parts Aster tataricus, 5 to 13 parts turmeric, 2 to 8 parts ginseng, 2 to 8 parts Hovenia dulcis Thunb. and a spice additive, the spice additive containing red dates.
[0007] The combination of the aforementioned ingredients not only achieves good efficacy but also avoids obvious toxic effects and side effects. The combination of ingredients can effectively implement a significant synergistic effect, resulting in beneficial liver protection and nutrition.
[0008] In some embodiments, the liver-protecting composition contains parts by weight of: 12 to 23 parts Lysimachia christinae, 7 to 13 parts Aster tataricus, 7 to 10 parts turmeric, 4 to 6 parts ginseng and 4 to 6 parts Hovenia dulcis Thunb.
[0009] In some embodiments, the liver-protecting composition contains parts by weight of: 17 parts Lysimachia christinae, 10 parts Aster tataricus, 9 parts turmeric, 5 parts ginseng and 5 parts Hovenia dulcis Thunb.
[0010] In some cases, Aster tataricus is roasted Aster tataricus.
[0011] In some embodiments, the liver-protecting composition also contains a pharmaceutically acceptable adjuvant.
[0012] In some embodiments, the dosage form of the liver-protective composition comprises a pill, a powder, an ointment, a tablet, a granule (electron), a lozenge, a capsule, an aqueous preparation or a tincture.
[0013] A preferred dosage form is the aqueous preparation or the tincture.
[0014] The aqueous remedy is an aqueous solution of a traditional Chinese medicine composition. The medicine is uniformly dispersed in water in the form of ions or molecules. The concentration of the remedy depends on the water solubility of the original medicine, which is usually its maximum solubility; the medicine is then diluted with water before use. To fully release the active ingredients from the medicine, the aqueous remedy can be prepared by boiling the medicine in hot water or by other methods, with the boiling process being carried out once, twice, or several times.
[0015] The tincture is a clear liquid formulation prepared by leaching or dissolving the drug in ethanol at a specific concentration; it can also be prepared by diluting the drug with a liquid extract. The tincture is taken orally or applied topically. Preparation of the tincture requires no heating, the tincture has relatively pure components with a high active ingredient content, and the tincture is administered in precise doses, is rapidly absorbed, and is suitable for preparing a formulation containing volatile or heat-labile components. Preferably, the tincture in the present invention is an orally administered tincture.
[0016] The present invention also provides a method for producing the aforementioned liver-protecting composition.
[0017] First, a simple composition is prepared. A further objective of the present invention is to provide a method for preparing the aforementioned liver-protective composition, comprising the following step: grinding and mixing the ingredients.
[0018] Secondly, a more complex preparation method is planned, which includes the following steps: (a) Boiling or scalding ingredients with water to obtain a boiled solution, or extraction using an aqueous ethanol solution to obtain an extract solution, and (b) Preparation of the decocted solution or the extract solution in a desired dosage form.
[0019] In some embodiments, the process for producing the liver-protecting composition includes: Crushing of Lysimachia christinae and Aster tataricus, addition of water to the resulting mixture, boiling of the resulting mixture twice for 1 to 2 hours each time, filtering of the boiled solution and combining of the filtrates to obtain the first filtrate; Crushing and mixing turmeric, ginseng and Hovenia dulcis Thunb., adding 60% to 80% ethanol 2 to 4 times for reflux extraction, combining the extracts and filtering the extracts to obtain the second filtrate; and Combine the first filtrate and the second filtrate, let the resulting mixture stand for 2 to 3 days, obtain a supernatant, extract the ethanol from the supernatant under reduced pressure and add the spice.
[0020] The present invention relates to a purely traditional Chinese medicinal product that is easy to prepare and convenient to take, has a good effect on protecting and nourishing the liver, and also has an effect on relieving the effects of alcohol. DETAILED DESCRIPTION
[0021] Detailed reference is made to embodiments of the present invention, and one or more examples are described below. Each example serves to illustrate, not to limit, the present invention. Indeed, a person skilled in the art can make various modifications and changes to the present invention without departing from its scope or spirit. For example, features shown or described as part of one embodiment can be used in another embodiment to create yet another embodiment.
[0022] Unless otherwise specified, all terms (including technical and scientific terms) used in the disclosure of the present invention have the same meaning as they would be generally understood by a person with ordinary knowledge in the field to which the present invention belongs. For better understanding of instructions relating to the present invention, the following definitions are used. The terms used herein in the description of the present invention serve only to describe certain embodiments and are not intended to limit the present invention.
[0023] The terms “comprise”, “include” and “contain” used in the present invention are synonymous and include or are open and do not exclude additional unnamed members, elements or process steps.
[0024] A range of values in the present invention, defined by endpoints, includes all values, fractions and the said endpoints that fall within the range.
[0025] A concentration value, which is the subject of the present invention, can fluctuate within a certain range. For example, the concentration value can fluctuate within a corresponding precision range. A precision range of 2%, for instance, allows fluctuations of ±0.1%. For a larger value, or one that does not require fine control, a larger fluctuation is also permissible. For example, a precision range of 100 mM can allow fluctuations of ±1%, ±2%, ±5%, etc. For the molecular weight, a fluctuation of ±10% is permissible.
[0026] In the present invention, terms such as "multiple" and "different" refer to two or more than two, unless otherwise specified.
[0027] Within the scope of the present invention, the technical features in the open-ended descriptions include both closed technical solutions consisting of the listed features and open technical solutions containing the listed features.
[0028] In the present invention, “preferred”, “better”, “advantageous” and “suitable” are intended only to describe embodiments or examples with better effect and should not be understood as limiting the scope of protection of the present invention.
[0029] The embodiments of the present invention are described in detail below with reference to examples. It should be understood that these examples are used only to illustrate the present invention, and it is not intended to limit the scope of the present invention. In the following examples, for a test method for which a specific condition is not specified, reference is preferably made to the instructions in the present invention, a test manual, a conventional condition from the prior art, another test method known from the prior art, or a condition from a manufacturer.
[0030] In the following specific examples, a measurement parameter relating to an ingredient may exhibit a slight deviation within a weighing accuracy range, unless otherwise specified. For temperature and time parameters, an acceptable deviation is permitted due to the instrument's testing accuracy or operational accuracy. Example 1
[0031] Weigh out: 10 g Lysimachia christinae, 15 g Aster tataricus, 5 g turmeric, 8 g ginseng, 2 g Hovenia dulcis Thunb. and 13 g red dates.
[0032] Preparation: Lysimachia christinae and Aster tataricus were crushed, the resulting mixture was mixed with water and boiled twice for one hour each time, the boiled solution was filtered and the filtrates were combined to obtain the first filtrate;
[0033] Turmeric, ginseng, and Hovenia dulcis Thunb. were ground and mixed, 80% ethanol was added for a double reflux extraction, and the extracts were combined and filtered to obtain the second filtrate; and
[0034] The first filtrate and the second filtrate were combined and left to stand for 2 days, then a supernatant was drawn off, ethanol was obtained from the supernatant under reduced pressure and red dates were added. Example 2
[0035] Weigh out: 25 g Lysimachia christinae, 5 g Aster tataricus, 13 g turmeric, 2 g ginseng, 8 g Hovenia dulcis Thunb. and 5 g red dates.
[0036] Preparation: Lysimachia christinae and Aster tataricus were crushed, the resulting mixture was mixed with water, boiled twice for 2 hours each time, the boiled solution was filtered and the filtrates were combined to obtain the first filtrate;
[0037] Turmeric, ginseng, and Hovenia dulcis Thunb. were ground and mixed, 60% ethanol was added for a quadruple reflux extraction, and the extracts were combined and filtered to obtain the second filtrate; and
[0038] The first filtrate and the second filtrate were combined and left to stand for 3 days, then a supernatant was drawn off, ethanol was obtained from the supernatant under reduced pressure, and red dates were added. Example 3
[0039] Weigh out: 12 parts Lysimachia christinae, 13 parts Aster tataricus, 7 parts turmeric, 6 parts ginseng, 4 parts Hovenia dulcis Thunb. and 10 parts red dates.
[0040] Preparation: Lysimachia christinae and Aster tataricus were crushed, the resulting mixture was mixed with water, boiled twice for 1 to 2 hours each time, the boiled solution was filtered and the filtrates were combined to obtain the first filtrate;
[0041] Crushing and mixing turmeric, ginseng and Hovenia dulcis Thunb., adding 60% to 80% ethanol 2 to 4 times for reflux extraction, combining the extracts and filtering the extracts to obtain the second filtrate; and
[0042] The first filtrate and the second filtrate were combined and left to stand for 2 to 3 days, then a supernatant was drawn off, ethanol was obtained from the supernatant under reduced pressure, and red dates were added. Example 4
[0043] Weigh out: 17 g Lysimachia christinae, 10 g Aster tataricus, 9 g turmeric, 5 g ginseng, 5 g Hovenia dulcis Thunb. and 9 g red dates.
[0044] Preparation: Lysimachia christinae and Aster tataricus were crushed, the resulting mixture was mixed with water, boiled twice for 2 hours each time, the boiled solution was filtered and the filtrates were combined to obtain the first filtrate;
[0045] Turmeric, ginseng, and Hovenia dulcis Thunb. were ground and mixed, 70% ethanol was added for triple reflux extraction, and the extracts were combined and filtered to obtain the second filtrate; and
[0046] The first filtrate and the second filtrate were combined and left to stand for 3 days, then a supernatant was drawn off, ethanol was obtained from the supernatant under reduced pressure, and red dates were added. Test example
[0047] The formulation prepared in Example 4 was used for a test on mice with acute liver damage caused by CCl4: Sixty mice were randomly divided into four groups of 15 mice each. Each group was administered via a gavage tube. A blind group and a modeling group received clean water, a test group received the formulation prepared in Example 4, and a positive group was given an effective dose of sunflower liver-protecting tablets to serve as a control group. The dosage was calculated for mice according to the Chinese Pharmacopoeia. The dosage for the positive group was 560 mg / kg and the dosage for the test group was 420 mg / kg.
[0048] In the present invention, olive oil was used to prepare a solution containing 1% CCl4. The solution was injected intraperitoneally into each mouse separately (10 ml / kg body weight) to create a model; only the blind group did not require modeling. One week after intragastric administration, the mice in the CCl4 modeling group, the test group, and the positive group were again injected intraperitoneally with the 0.1% CCl4 oil solution to create the model. Subsequently, blood samples were taken from each mouse within 18 hours using an orbital blood collection method, and liver function indicators (AST and ALT) were determined using conventional methods. The results are shown in Table 1. Table 1 group Number of animals ALT (U / L) AST (U / L) Blind group 10 37,2±7,4** 30,6±4,33** Modeling group 10 397,5±81,6 141,6±32,7 Test group 10 154,2±29,8** 82,90±10,75** Positive Group 10 234,55±52,01** 98,73±12,93**
[0049] Note: Compared to the modeling group, *P<0.05 and **P<0.01.
[0050] The foregoing results show that the drug according to the invention was able to significantly reduce ALT activity, and there was a very significant difference (P<0.01) compared to the model group; and
[0051] Samples at all three dosages were able to reduce ALT activity, and there was a highly significant difference (P<0.01) between the three dosed groups and the modeling group. The test results indicate that the sample has a significant protective effect against liver damage and exhibits a significant hepatoprotective effect.
[0052] The preceding embodiments describe only a few examples of the present invention. The descriptions are relatively detailed and specific, but cannot be interpreted as limiting the scope of the present invention. It should be noted that a person skilled in the art could also make several variations and improvements without departing from the concept of the present invention. These variations and improvements all fall within the scope of protection of the present invention.
Claims
[1] A liver-protecting composition containing in parts by weight: 10 to 25 parts Lysimachia christinae, 5 to 15 parts Aster tataricus, 5 to 13 parts turmeric, 2 to 8 parts ginseng, 2 to 8 parts Hovenia dulcis Thunb. and a spice additive, the spice additive consisting of red dates. [2] The liver-protecting composition according to claim 1, comprising in parts by weight: 12 to 23 parts Lysimachia christinae, 7 to 13 parts Aster tataricus, 7 to 10 parts turmeric, 4 to 6 parts ginseng and 4 to 6 parts Hovenia dulcis Thunb. [3] The liver-protecting composition according to claim 2, comprising in parts by weight: 17 parts Lysimachia christinae, 10 parts Aster tataricus, 9 parts turmeric, 5 parts ginseng and 5 parts Hovenia dulcis Thunb. [4] The liver-protecting composition according to any one of claims 1 to 3, wherein the Aster tataricus is roasted Aster tataricus. [5] The liver-protecting composition according to claim 4, which also contains a pharmaceutically acceptable adjuvant. [6] The liver-protecting composition according to claim 4, wherein the dosage form is a pill, a powder, an ointment, a tablet, a granule, a lozenge, a capsule, an aqueous agent or a tincture. [7] Method for producing the liver-protecting composition according to any one of claims 1 to 6, comprising the following step: grinding and mixing the ingredients. [8] Method for producing the liver-protecting composition according to any one of claims 1 to 6, comprising the following steps: (a) Boiling or scalding ingredients with water to obtain a decoction solution, or carrying out an extraction using an aqueous ethanol solution to obtain an extract solution; and (b) Preparation of the decocted solution or the extract solution in a desired dosage form. [9] Method for producing the liver-protecting composition according to claim 8, comprising: Crushing of Lysimachia christinae and Aster tataricus, addition of water to the resulting mixture, boiling of the resulting mixture twice for 1 to 2 hours each time, filtering of the boiled solution and combining of the filtrates to obtain the first filtrate; Crushing and mixing turmeric, ginseng and Hovenia dulcis Thunb., adding 60% to 80% ethanol 2 to 4 times for reflux extraction, combining the extracts and filtering the extracts to obtain the second filtrate; and Combine the first filtrate and the second filtrate, let the resulting mixture stand for 2 to 3 days, obtain a supernatant, extract the ethanol from the supernatant under reduced pressure and add the spice.