System for extracting Achyranthes aspera for applications against obesity

Abstract

A system for the extraction of bioactive phytochemicals from Achyranthes aspera seeds, comprising an extraction chamber, a solvent supply unit for providing hydroalcoholic mixtures and a filtration module for producing a clarified extract suitable for applications in the field of obesity treatment.

Description

Field of invention

[0001] The invention relates to a system for extracting bioactive phytochemicals from the seeds of Achyranthes aspera for the production of therapeutic extracts that are effective for the prevention of obesity and for the treatment of metabolic disorders. Background of the invention

[0002] Obesity is a growing global health problem associated with metabolic disorders, oxidative stress, and enzyme dysregulation, increasing the risk of cardiovascular disease, diabetes, and related conditions. Existing pharmaceutical treatments often cause side effects, creating a need for safe, plant-based alternatives that can counteract oxidative imbalance and metabolic enzyme activity. Achyranthes aspera is a traditional medicinal plant containing phenols, flavonoids, tannins, alkaloids, and saponins, all of which exhibit antioxidant and metabolic regulatory potential. However, conventional extraction methods are not precise enough, lacking optimal solvent ratios and controlled process parameters to obtain bioactive components in high yield.Furthermore, most available technologies do not offer standardized extraction conditions that allow for the consistent production of a therapeutically effective composition. Therefore, there is a need for an improved extraction system for the production of hydroalcoholic seed extracts of Achyranthes aspera that enables a high yield of phenols and flavonoids, increased antioxidant activity, and improved inhibitory effects on obesity-associated pancreatic lipase and α-amylase.

[0003] Summary of the Invention: The invention provides a system for the controlled extraction of constituents from Achyranthes aspera seeds to produce a standardized bioactive extract for the treatment of obesity. The system regulates the solvent composition, extraction parameters, mixing conditions, and filtration steps to achieve an optimal yield of phytochemicals. Through a controlled hydroalcoholic solvent delivery mechanism, the system enables the production of extracts with varying ethanol concentrations. A 25% hydroalcoholic formulation yields the highest content of phenols, flavonoids, and tannins. To ensure the therapeutic potential of the extract, integrated screening, antioxidant, and enzyme inhibition analyses can be performed.

[0004] The system also delivers an optimized extraction yield, exhibiting strong antioxidant properties, low IC50 values ​​in DPPH and ABTS assays, and significant inhibition of pancreatic lipase and α-amylase activity. The extract obtained from this system has been confirmed safe in in vitro and in vivo toxicity studies and has demonstrated efficacy in weight reduction and serum profile improvement in mice with high-fat diet-induced obesity. The system thus ensures the consistent production of a plant-based, non-toxic extract suitable for the treatment of obesity through targeted modulation of oxidative stress and metabolic enzymes. Detailed description

[0005] The system includes an extraction chamber for receiving Achyranthes aspera seeds. Within this chamber, the seeds are crushed in a controlled manner and prepared for solvent extraction. A solvent supply unit delivers water and ethanol in precise concentrations, enabling the preparation of various hydroalcoholic mixtures for extraction. The system is designed to use an optimized 25% hydroalcoholic solvent, which has been proven to maximize the content of phenols, flavonoids, and tannins in the resulting extract.

[0006] In the extraction chamber, the seed material is mixed and stirred in a controlled manner to improve solvent penetration and facilitate the release of phytochemicals. The chamber maintains constant temperature and time to ensure consistent extraction performance. After completion of the extraction cycle, the mixture is transferred to a filtration module to remove plant residues and obtain the clarified extract.

[0007] The system also includes a concentration / solvent evaporation component that allows for adjustment of the extract volume to achieve the desired phytochemical concentration. A phytochemical analysis module can be integrated to verify the presence of alkaloids, saponins, phenols, flavonoids, and tannins. These controls ensure that each batch produced meets the quality criteria for therapeutic use.

[0008] The system is compatible with analytical instruments for determining antioxidant activity. The 25% hydroalcoholic extract produced by the system exhibits strong antioxidant potential with low IC50 values ​​in DPPH and ABTS tests, indicating a high capacity for free radical scavenging. This antioxidant activity supports the extract's role in reducing cellular oxidative stress.

[0009] The extract produced in the system also exhibits a significant inhibitory effect on pancreatic lipase and α-amylase, suggesting a potential for reducing fat and carbohydrate breakdown during digestion. These functional properties make the extract suitable for applications in the treatment of obesity.

[0010] For further validation, the system supports cellular antioxidant assays using cell lines such as 3T3-L1 to confirm the reduction of markers of oxidative stress. Subsequent LC-MS analysis reveals the presence of compounds such as stigmasterol, dolichol phosphate, and 9'-hydroxy-9'-apo-ε-carotene-3-one, which contribute to biological activity.

[0011] Toxicity studies demonstrate that the extracts produced with this system are non-toxic and show no adverse effects in cell lines or mouse models in in vivo experiments. The system's ability to ensure reproducible extraction quality guarantees the safety of the extract.

[0012] Efficacy tests on mice obese due to a high-fat diet confirm that the extracted substance offers measurable biological benefits. The treated mice exhibit significant weight loss, improved lipid and biochemical markers, and an overall better metabolic profile.

[0013] Through precise control of the solvent composition, extraction parameters, and post-processing steps, the system ensures the production of Achyranthes aspera extract with high yield and consistent therapeutic efficacy. The invention thus provides a reliable and standardized system for the production of herbal anti-obesity preparations.

Claims

[1] A system for extracting bioactive phytochemicals from Achyranthes aspera seeds, comprising an extraction chamber, a solvent supply unit for providing hydroalcoholic mixtures and a filtration module for producing a clarified extract suitable for applications in the field of obesity treatment. [2] System according to claim 1, wherein the solvent supply unit is configured to produce a 25% hydroalcoholic solvent which enables a maximum yield of phenols, flavonoids and tannins. [3] System according to claim 1, wherein the produced extract has antioxidant activity characterized by low IC50 values ​​in DPPH and ABTS tests and by inhibition of pancreatic lipase and α-amylase. [4] System according to claim 1, wherein the safety of the produced extract has been confirmed by in vitro and in vivo toxicity studies and its efficacy in weight reduction and improvement of metabolic markers in obese persons has been demonstrated.