METHOD FOR GEL ELECTROPHORESIS WITH IN-SITU CALIBRATION
Patent Information
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- HELENA LAB CORP
- Filing Date
- 2013-05-29
- Publication Date
- 2026-04-15
AI Technical Summary
Conventional electrophoresis methods lack the ability to provide absolute quantitation of separated fractions within an electrophoretic matrix, relying on external methods for concentration determination and suffering from inter-sample variability and intra-sample errors, particularly at the microvolume level.
A method involving in-situ calibration, where a test sample is combined with a calibrating sample containing a known concentration of a calibrator, allowing simultaneous electrophoresis and detection of both samples in a common separation lane, enabling direct measurement of unknown component concentrations relative to the internal reference standard.
This approach eliminates the need for external testing, reduces inter-method variability, and enhances within-sample control and confidence by providing accurate, absolute quantitation of electrophoretically separated components.