EXCLAIM OF HATENE-CONTAINING POLYPEPTIDES
Patent Information
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- IMPOSSIBLE FOODS INC
- Filing Date
- 2014-09-11
- Publication Date
- 2026-06-10
AI Technical Summary
Existing methods struggle to effectively produce heme-containing polypeptides in recombinant bacterial cells, particularly due to challenges in secreting and folding these proteins using the SEC and TAT systems, as the cofactor must be inserted and remain associated throughout the secretion process in its native configuration.
A recombinant bacterium cell, such as Bacillus subtilis, is engineered with a fusion polypeptide comprising a signal peptide and a heme-containing polypeptide, where the signal peptide is operably linked to facilitate secretion, and upon secretion, the signal peptide is removed, allowing the heme-containing polypeptide to be properly secreted and folded.
The method enables efficient secretion and proper folding of heme-containing polypeptides, such as androglobin and myoglobin, in bacterial cells, achieving high activity levels comparable to wild-type polypeptides.