BACTERIAL LEADER SEQUENCES FOR PERIPLASMIC PROTEIN EXPRESSION
DE602018082764T2Active Publication Date: 2025-06-18PELICAN TECHNOLOGY HOLDINGS INC
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Patent Information
- Application Number
- DE602018082764
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2017-10-27
- Filing Date
- 2018-10-17
- Publication Date
- 2025-06-18
- Estimated Expiration
- 2038-10-17
AI Technical Summary
Technical Problem
Current methods for producing recombinant proteins in bacteria often result in improperly folded, aggregated, or inactive proteins due to the formation of inclusion bodies and the bacterial cytoplasm's resistance to disulfide bond formation.
Method used
The use of novel secretion signals, such as the AnsB secretion signal, to direct recombinant proteins to the periplasm of Gram-negative bacteria, where proper folding and disulfide bond formation can occur, and the proteins can be efficiently secreted into the extracellular environment.
Benefits of technology
This approach increases the yield of properly folded, soluble, and active recombinant proteins, reduces the formation of inclusion bodies, and facilitates more efficient purification and recovery of the proteins.
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