Photoacoustic method for characterizing a plant

Abstract

A photoacoustic method for characterizing a plant (10), the method comprising applying laser radiation (105) to a surface of the plant material, recording by a microphone (175) sound waves appearing by photothermal effect in a photoacoustic cavity (155) positioned around said surface of the plant, the laser radiation (105) having been applied to said surface through the cavity (155). The plant surface is a surface of a stem (or root or petiole) (11) of the plant, and the method comprises inserting a section (16) of said stem (11) into a support comprising the cavity (155) equipped with a microphone sensor (175) and a window (170) for transmitting the laser power, with sealing means (160, 162) placed around the stem (11) at each of the two ends of said section (16). Abbreviated figure: 1

Description

Title of the invention: Photoacoustic method for characterizing a plant Technical context

[0001] The invention falls within the field of apparatus and methods for the exploration of living things, in particular the plant world and more specifically the in vivo monitoring of plant activity.

[0002] It exploits photoacoustics—also called optoacoustics—that is, the observation of mechanical waves induced in a body illuminated by electromagnetic radiation. The phenomena underlying this generation are photothermal activity: the incident waves heat the body, and thermal expansion: a temperature increase causes expansion. Time-varying incident light is used to create expansion, which is followed by contraction.

[0003] The ability to absorb light depends on the composition of matter and the electromagnetic background. Photoacoustics is therefore well-suited for spectroscopic manipulations. We are particularly interested in a spectroscopic approach in the mid-infrared (MIR) spectral range. This range of the electromagnetic spectrum provides a specific signature for molecules. Acquiring spectra then allows us to determine the molecular composition of the substances studied and, potentially, to quantify the chemical species present.

[0004] In recent years, work has initially focused on the development of miniature gas sensors based on this principle of mid-infrared spectroscopy by photoacoustic transduction. These developments have then been applied to the characterization of liquids and solids, and have led, for example, to the development of a sensor capable of monitoring a person's blood glucose level non-invasively.

[0005] Mid-infrared spectrometry without acoustic transduction is also used in the field of plant biology. Indeed, work already carried out on plants uses micro-spectrometry of the Fourier transform infrared (FTIR) type, which makes it possible to establish maps at the scale of tens of microns of plant tissues sampled in thin sections or reduced to powder, for example for the analysis of the composition of cell walls, which is of interest for understanding intracellular communication and, more specifically, for the wood industry.

[0006] Research has also been carried out to develop the use of photoacoustics for the study of plants, again with infrared spectroscopy. It has It has been proposed to modify the modulation frequency of the optical beam to deduce the chemical composition in the thickness of plant leaf samples.

[0007] The use of open cavities placed on plant leaves has been proposed. Such arrangements for the in vivo study of photosynthesis are also known from Pereira et al., Meas Sci. Technol. 3, 1992, 931, and from Mesquita et al., Instrumentation Science and Technology, vol. 34, 33, 2006. These arrangements involve placing an open cavity with its mouth pressed against a plant leaf, thus closing the cavity. The other side of the leaf is exposed to visible light (white light and monochromatic light at 680 nm or 650 nm), guided by optical fiber. The cavity contains an electret microphone. These experiments, performed with visible wavelengths, aim to monitor photosynthetic activity in vivo. However, the measurement is invasive (the leaf is covered) and therefore does not allow for prolonged monitoring without impacting the plant's activity.

[0008] In the article Helfter C. et al. (2007) Tree Physiology, vol 27, 169, the response of an intact plant stem to a near-infrared (812 nm) heat source is observed using an infrared camera. The approach is non-invasive, but the characterization does not provide access to chemical information.

[0009] It has also been proposed to examine living tissues in the mid-infrared, by attenuated total reflectance FTIR measurement (ATR-FTIR), in Zhang et al. 2023, Scientific Reports, vol 13. The limitations of the method presented in this article are the temporal tracking which cannot be fine without disturbing the plant, and the restriction of the area examined to the first few microns of the sample.

[0010] Finally, in Gaoqiang L., et al., 2020, Spectrochimica Acta Part A, vol 228, a mid-infrared spectrometry approach using photoacoustics to investigate the chemical composition in plant leaf samples was presented. Description of the invention

[0011] It is now proposed, below, to place a photoacoustic cavity on the stem of a plant for its study and monitoring.

[0012] To overcome the shortcomings presented by these prior art systems and methods, a photoacoustic method for characterizing a plant is proposed, the method comprising the application of laser radiation to a surface of plant material, a recording by a microphone of sound waves appearing by photothermal effect in a photoacoustic cavity positioned around said surface of the plant, the laser radiation having been applied to said surface through the cavity.

[0013] Moreover, remarkably, the plant surface is the surface of a stem, root, or petiole of the plant, the plant being intact and typically living and growing, and the method comprises inserting a section of said stem, root, or petiole into a support comprising a cavity equipped with a microphone sensor and a window for transmitting the power of the laser beam, with sealing means placed around the stem, root, or petiole at each of the two ends of said section. This method is non-invasive.

[0014] These principles make it possible to explore the stem, root or petiole of a plant, for example a stem of 2 to 3 mm in diameter, and to probe in particular the phloem and the xylem during the activity of the plant, at various wavelengths.

[0015] Various optical sources for photoacoustics can be used. Monitoring over a timescale relevant to plant development makes it possible to understand the associated dynamics and to take appropriate action.

[0016] More particularly, a system is proposed including a sensor to monitor plant activity by photothermal analysis in the mid-infrared.

[0017] Thanks to photothermal monitoring in the mid-infrared (MIR), the richness of mid-infrared spectroscopy is brought to bear, as photothermal imaging allows the sample to be scanned through its thickness – typically to a depth of around one hundred microns, the exact achievable depth depending on the chemical composition of the plant being studied and the range of wavelengths used in the MIR. Thus, temporal resolutions on the order of a second can be obtained for monitoring over long timescales, for example, several days.

[0018] A mid-infrared spectroscopic measurement of the cell wall of a plant's stem, root, or petiole is therefore performed. This measurement is carried out in vivo, is minimally invasive, and can be quasi-continuous. It allows for the detection, with a very high probability, of a signal specific to the plant's activity. This measurement has strong potential to aid in understanding the processes occurring in plants, thus opening up a wide field of fundamental research and practical applications.

[0019] Preferably, the environment is measured simultaneously, and the optical power of the laser is continuously measured. In both cases, this is done to correct the measurements or to identify correlations, which may or may not be specific to the activity of the plant being studied.

[0020] The multifrequency modality of photoacoustics allows the depth of characterization of the sample to be varied. Thus, it makes it possible to scan the sample in its depth, and possibly to distinguish processes occurring at different depths but within the outermost 150 pm layer of the stem, root or petiole.

[0021] Thus, advantageously and optionally:

[0022] - the support may consist of a set of two opposing jaws forming vise and each equipped with a sealing gasket;

[0023] - laser radiation can be applied as a continuous wave with modulation amplitude at several frequencies; modulation frequencies can thus be chosen, for example from 100 to 1000 Hz, which allows some to probe deeper layers of the stem, root or petiole, and others to probe more superficial layers;

[0024] - the laser radiation can be at a frequency in the mid-infrared corresponding to an absorption band of a molecule of interest for monitoring plant growth. For example, glucose at 1036 cm¹ might be of interest. The laser radiation can also consist of a set of laser sources at different optical wavelengths relevant for plant analysis.

[0025] - the recording can be carried out for a duration of approximately 1 second, with Sampling at a frequency of around 100 kHz is common, but generally a photoacoustic spectrum (with several modulation frequencies) is recorded using one or more lasers, the recording method being adapted to the specific case study. The spectrum can be recorded by taking multiple measurements at different modulation frequencies (the duration then depending on the desired signal-to-noise ratio), or in pulsed mode.

[0026] - the method may consist of continuously tracking the plant by regular repetitions of the laser application and the associated subsequent measurement for at least one day and one night.

[0027] - the measurement can be demodulated to identify a phase and a modulus, and thus to perform a photoacoustic spectrum.

[0028] - the plant may be alive and is little affected by carrying out the measurement - it is a A major advantage of the method is that there is no disruption to respiration or photosynthesis activity, and the measurement can nevertheless be carried out over a very long period, in a non-invasive manner.

[0029] The invention also consists of a photoacoustic equipment for characterizing a plant, the equipment comprising means for applying laser radiation, a photoacoustic cavity configured so that the laser radiation can be applied through the cavity, and means for recording by a microphone sound waves appearing by photothermal effect in the photoacoustic cavity.

[0030] The equipment further comprises a support including the cavity, the support also including an insertion space for a section of a plant stem, root, or petiole, and sealing means for the cavity at each of the two ends of said section. The sealing joints are preferably specifically tailored to the dimensions of the stem, root, or petiole being tested. The means for applying a Laser radiation can also include a tunable laser, or several optically coupled monochromatic lasers.

[0031] This allows for the development of a system permanently installed on the plant without impacting its development

[0032] The applications are numerous, particularly in connection with the climate crisis and the need to monitor plant activity to help the agri-food sector understand the state of its production and anticipate the adaptation of plants to the environmental changes they will experience, as well as the likely future lack of available resources.

[0033] Optionally, the support consists of a set of two opposing jaws forming a vise and each equipped with a thinned circular sealing gasket to form with the opposite gasket a set of two opposing passages on either side of the cavity for the placement of the stem, root or petiole, with sealing around the stem, root or petiole. Brief description of the figures

[0034] Fig. 1 shows a diagram of the device of the invention.

[0035] Figures 2 to 5 show three-dimensional views of an embodiment of the device according to the invention.

[0036] Fig. 6 shows a plant stem and the spaces of it which are explored using the principles of the invention.

[0037] Figures 7 and 8 show measurements obtained with the device according to the invention. Description related to the drawings

[0038] We wish to follow the growth dynamics of a plant, for example the dynamics of the transport and storage of sugars, and therefore predict its efficiency, so that, for example, the agricultural sector can have a production of better nutritional quality while minimizing external inputs.

[0039] We also wish to detect the presence of chemicals related to changes in air or soil, as plants act as a kind of concentrator, which can be used to detect subtle variations in the environment. Specifically, we want to track the relevant chemical compound within the plant over time. To do this, we use the absorption spectrum associated with this compound, as well as the spectra of compounds likely to generate an equivalent photoacoustic signal. For the wavelength(s) that appear most suitable for detecting the compound, we evaluate the amplitude of the signal associated with variations in its concentration, while excluding signal variations linked to concentration variations of any interfering compounds.

[0040] [Fig.1] Fig.1 shows an assembly implementing the principles of the invention for tracking plant 10.

[0041] A laser 100 which is a quantum cascade laser emitting in the mid-infrared produces a laser beam 105 which is treated by a converging lens 110.

[0042] The optical source is a quantum cascade laser (QCL) emitting in the mid-infrared, within a restricted spectral range, or over a wider range with the possibility of wavelength tuning. A particular case is that of a monochromatic QCL DFB (Distributed Feedback Laser). Another example is an external cavity QCL. It is also possible to have several QCL and / or DFB lasers coupled by an optical coupler system and then switched on intermittently.

[0043] A watertight housing 150 defines a cavity 155, having two openings 160 and 162 facing each other on two opposite walls of the watertight housing 150. The watertight housing 150 is formed of two parts (not visible in the figure) movable relative to each other and joined to form the shell of the cavity and each of the two openings 160 and 162. One of the two parts includes a window 170 transparent to waves in the mid-infrared range, for example, a silicon or germanium window with a suitable anti-reflective coating. One of the two parts includes, facing its convex side, a microphone 175 (mounted in an electronic board) for performing photoacoustic measurements in the cavity and having a power supply from outside the housing and a means of transmitting the captured data to an external computer.The part of the housing that accommodates the microphone 175 and its electronic board may be the same as the one that accommodates the window 170, as shown in the figure, but it is also possible that it is the other one.

[0044] The plant 10 has a stem 11 separating lower parts, typically the roots, from upper parts, typically the leaves, fruits, and flowers. For tracking the plant 10, the stem is positioned so as to pass through openings 160 and 162 before the two movable parts are joined to form the cavity. Sealing means are also placed around the stem 11 in each of the openings 160 and 162 to ensure a seal around it. Between the two openings 160 and 162, a section 16 of the stem extends—it should be longer than the height separating the openings 160 and 162. The measurement can be performed not only on a stem, but also on any cylindrical element of the plant: stem, petiole, or root.

[0045] The laser beam 105 is directed and the lens 110 is positioned so that the laser power passes through the window 170 and is focused on the rod 11, in the cavity 155.

[0046] The photoacoustic response of the plant is measured over a long period, and the environment is measured simultaneously, and measurements are also taken continuously. The optical power of the laser is measured. Measurements of the plant's response are corrected according to the environment and the laser, and correlations are sought. The inputs provided to the plant are also adjusted to improve crop yield.

[0047] [Fig.2] The following figures show several three-dimensional views of a photothermal device for indirect acoustic detection (the acoustic wave is transmitted through the gas of the cavity, in this case air to the microphone), in accordance with the principles of the invention.

[0048] The photoacoustic cavity is clamped onto the stem 11 of the plant, thus placing the stem inside the cavity. The cavity is sealed using suitable means that have minimal impact on the plant. For example, the sealing means are custom-designed silicone gaskets, tailored to the diameter of the stems to be analyzed. These silicone gaskets seal the photoacoustic cell without damaging the plant. The microphone detects the acoustic wave induced by the photothermal effect within the photoacoustic cavity.

[0049] The watertight housing 150 is composed of two jaws 151 and 152, each forming one of the housing parts mentioned above. In [Fig.2], the two jaws are placed opposite each other, without being in contact with each other.

[0050] The jaw 151 has a general rectangular plate shape, one of whose faces (not visible in [Fig.2]) has recesses to form on the one hand the cavity 155 and on the other hand the openings 160 and 162. The other face (visible in [Fig.2] and which can be described as the rear face) is less functionalized and will not be discussed here.

[0051] The other jaw, namely jaw 152, also has a general rectangular plate shape, thicker than the plate of jaw 151, but with the same lateral dimensions as the rectangle of jaw 151, the assembly of the two jaws 151 and 152 being made by placing opposite sides of the same dimensions.

[0052] The jaw 152 has on one face (visible in [Fig.2]) recesses in order to form, with corresponding recesses of the other jaw, the cavity 155 and the openings 160 and 162. On its other face (not visible in [Fig.2], and which can be described as the rear face), the jaw carries a sufficiently large offset structure 153 for the optical processing of the laser light and for the fixing of the housing for example with an arm or a foot.

[0053] The cavity 155 is a small space compared to the overall structure formed by the two jaws. It is formed by recesses in the two plates, located at the center of the rectangles that constitute the faces of these plates, which are joined together once the cavity is formed. The recesses in the two jaws are of different sizes: the cavity is larger on one side of the plane where the two jaws meet than on the other, specifically in jaw 152.

[0054] The cavity 155 is more precisely a space delimited by a wall having the shape of a cylinder of revolution with an axis perpendicular to the planes of the two plates. This wall is formed in the jaw 152. The cavity is also delimited by bottoms, one in one jaw, the other opposite in the other jaw.

[0055] Openings 160 and 162 are defined by corresponding recesses in the two plates, which together have the shape of a cylinder of revolution with their axis parallel to the plane of the plates, and more precisely, in the embodiment shown in the figure, parallel to the shorter side of the rectangles forming the jaws. These recesses forming openings 160 and 162 are formed half in one jaw and half in the other jaw. The two openings 160 and 162 have the same axis and the same diameter, the cavity 155 being located midway between the first and second openings. The openings are through-holes on both sides of the interface between the two jaws.

[0056] The face of the jaw 152 further includes an annular recess around the cylindrical wall forming the cavity 155. It has its counterpart in the face of the jaw 151 against which the jaw 152 is pressed when the cavity is closed, so as to form an annular space 168 for the placement of an O-ring between the two jaws. The annular space 168 meets the openings 161 and 162, and extending from these, the cylindrical wall separating the cavity 155 from the annular space 168 is pierced by a cutout that extends the openings 161 and 162 in such a way that the plant stem can be placed in the openings 161 and 162 while extending into the cavity and passing twice through the annular space 168, on either side of the cavity 155.

[0057] The offset structure 153 further includes a through opening 111 for the passage of laser light which is placed in the extension of the cavity 155.

[0058] [Fig. 3] In [Fig. 3], which, like [Fig. 2], is a three-quarter view, the two jaws are positioned opposite each other and in contact, thus forming a cavity that is therefore invisible. The photoacoustic sensor or microphone 175 and its electronic board for monitoring plant activity are shown. This equipment is embedded in the jaw 152 and is flush with its rear face. A window 180 made of a material transparent to laser wavelengths is also shown; this window is flush with the middle of the rear face of the jaw 152, not far from the electronic board of the microphone 175.

[0059] The offset structure 153 has, visible from the viewing angle, a mounting space 112 for the converging slit. The through-hole 111 for the passage of the laser light is located behind this mounting space 112. The window 180, the mounting space 112, and the opening 111 are aligned.

[0060] [Fig.4] In [Fig.4], the configuration of [Fig.3] is shown in side view, in the direction of the openings 161 and 162 which are aligned perpendicularly to the section view along a plane passing through the cavity 155 in a plane coinciding with the optical axis.

[0061] The microphone 175 is visible with its electronic board as well as the through opening 111 for the passage of laser light and the fixing space 112 for the converging lens.

[0062] The annular space 168 appears in two fragments, on either side of the cavity 155. It is symmetrical on either side of the interface between the two jaws (like the openings 161 and 162, but unlike the cavity 155 which extends more into the jaw 152.

[0063] The cavity 155 is visible: it extends over approximately four-fifths of the thickness of the jaw 152, between the surface of adhesion of the two jaws and the window 180 made of material transparent to the wavelengths of the laser which is present in the middle of the rear face of the jaw 152 in the extension of the cylinder constituting the cavity 155.

[0064] The jaw 152 further includes in its internal volume a conduit 182 which allows a sound propagating in the cavity 155 to be captured by the microphone 175. The conduit 182 opens into the cavity 155 and extends to the location of the microphone 175 sensor.

[0065] [Fig. 5] Figure 5 shows the two jaws 151 and 152 separated from each other. The offset structure 153 is only visible in the background, but the cavity 155 is visible. Two annular seals 190 and 192 are present in the two halves of the annular recess 168, respectively on the surface of jaw 151 and on the surface of jaw 152. These seals are made of silicone and have been formed with a thinning to be placed in line with the openings 161 and 162 so as not to excessively compress the plant stem.

[0066] [Fig. 6] [Fig. 6] is a visible-light optical microscope photograph of a cross-section of the stem of the plant used during tests. The image shows the area probed in the mid-infrared.

[0067] The plant is a wandering Jew with the biological name Tradescantia zebrina. The diameter of the stem is slightly less than 2 mm. The annular zone in which the phloem and xylem are located is indicated by reference 300, and the penetration depth of a laser beam at 1036 cm¹ is indicated by reference 310: it can be seen that the laser explores a significant external part of zone 300.

[0068] Liquid water is a strong absorbent in the mid-infrared, and there is a lot of water in cells. It is therefore common practice to begin by measuring variations in water content.

[0069] Water also directly hinders the possibility of examining plants deeply, particularly beyond 150 pm below the surface. It is therefore more advantageous to work on young and / or thin stems and / or roots, with a diameter of less than 1 mm.

[0070] At 1036 cm', after water, it is likely that the signature of the components of the cell walls, mainly composed of cellulose and lignin, will be detected.

[0071] The measurement method is adapted to make a relative measurement and to follow the evolution of the composition being probed as a function of time.

[0072] It is indeed relevant to use the method to monitor the nature of the fluids circulating in the plant's vascular system. Since the latter is located primarily at the periphery of plants, it is accessible to photothermal analysis.

[0073] [Fig.7] A series of measurements was carried out with the system described in the previous sections, visible in figures 1 to 6, according to two protocols.

[0074] Protocol 1 is a continuous measurement over several days with a plant installed in the device. The presence of the stem ensures that the cavity is airtight, and the photoacoustic signal is captured by the microphone.

[0075] Protocol 2 is a continuous measurement under the same conditions as Protocol 1, but without the plant. The photoacoustic cell is, however, blocked at the channel intended for the stem to ensure the generation of the photoacoustic signal. Protocol 2 serves as a reference to verify that the signals detected during Protocol 1 are induced by the plant and not by a drift in the system, or a sensitivity to changes in the environmental parameters of the experiment.

[0076] The experimental conditions for carrying out these tests are the use of an Alpes QCL laser, emission at 1036 cm⁻¹, with a mean current laser operating point iO = 0.710 A, a modulation amplitude il = 0.10 A, and a temperature regulated at 25°C. The laser power is estimated at 5 mW at the plant stem. The modulation frequencies are 107, 134, 168, 210, 162, 328, 411, 514, 643, 805, and 1007 Hz (therefore, 11 different frequencies), and the system performs a scan composed of these 11 frequencies. The scan is repeated every 4 minutes.

[0077] Each measurement in a scan consists of a one-second measurement with sampling at 100 kHz. The signal is then demodulated with a square wave window at the laser modulation frequency (so-called If demodulation).

[0078] The entire procedure is carried out in the absence of natural light, but in the presence of artificial light during the day. The temperature is regulated. The sample is a stem of the plant Tradescantia zebrina for protocol 1. The reading lasts 165 hours for protocol 1 and 114 hours for protocol 2.

[0079] Raw and processed data from the two experiments were examined. The data were filtered with a median filter of width lh20. High noise was reduced frequency by increasing the integration time of synchronous detection. A median filter is a suitable solution for removing this type of noise. Then the data are debiased by subtracting a common low-frequency component and a slow drift, on the scale of several days – defined by averaging all the data at the different frequencies, and adding a second-order polynomial. We are also interested in the filtered and debiased data in the form of spectrograms.

[0080] A module for monitoring the environmental conditions of the monitoring is installed. The observed temporal trends resemble xylem flow measurements taken by other means and can be linked to a plant process related to light exposure. These trends may be an increase in the velocity of fluid circulation in the xylem, and therefore a change in the plant's thermal properties throughout the day, or an increase in the volume of water or the concentration of absorbing elements in the laser's field of view, again throughout the day.

[0081] Figure 7 shows the evolution of a portion of the photoacoustic signal (here, the 107 Hz phase in arbitrary units and after filtering and debiasing) as a function of time during a one-week experiment (the seven days of the week are marked by vertical dashed lines). Daily cycles are observed, which are interrupted at the weekend when the plant is no longer exposed to light.

[0082] [Fig.8] Fig.8 shows the evolution of different parts of the photoacoustic signal (here the phases at the 11 frequencies mentioned above) as a function of time during an eight-day follow-up (the days of the week are marked by vertical dotted lines).

[0083] A spatio-temporal dynamic is observed in the phases and modules in the plant and in particular a process which would occur from the inside to the outside (or vice versa) of the plant.

[0084] This is particularly visible in the 900 time zone which spans a little over half a day, and during which there is a signal peak which gradually shifts from high to low frequencies.

[0085] The system uses, in one embodiment, a wavelength-tunable source, in order to spectrally characterize the sample and define the appropriate wavelengths according to the intended applications.

[0086] The invention has been described with the continuous-wave electromagnetic emission method with modulated intensity. However, as mentioned above, it can be implemented with a pulsed method.

Claims

Demands

1. A photoacoustic method for characterizing a plant (10), the method comprising applying laser radiation (105) to a material surface of the plant, recording by a microphone (175) sound waves appearing by photothermal effect in a photoacoustic cavity (155) positioned around said surface of the plant, the laser radiation (105) having been applied to said surface through the cavity (155), the method being characterized in that the plant surface is a surface of a stem, root or petiole (11) of the plant, and the method comprising inserting a section (16) of said stem, root or petiole (11) into a support comprising the cavity (155) equipped with a microphone sensor (175) and a window (170) for transmitting the laser power, with sealing means (160, 162) placed around the stem, root or petiole (11). each of the two ends of said section (16).

2. Photoacoustic method for characterizing a plant according to claim 1, characterized in that the support is composed of a set of two opposing jaws (151, 152) forming a vise and each equipped with a sealing gasket.

3. Photoacoustic method for characterizing a plant according to claim 1 or claim 2, characterized in that the laser radiation (105) is applied as a continuous wave with amplitude modulation at several frequencies.

4. Photoacoustic method for characterizing a plant according to any one of claims 1 to 3, characterized in that the laser radiation (105) is at a frequency in the mid-infrared corresponding to an absorption band of a molecule of interest for monitoring plant growth.

5. Photoacoustic method for characterizing a plant according to any one of claims 1 to 4, characterized in that it consists of tracking the plant continuously by regular repetitions of the application of the laser and the associated measurement for at least one day and one night.

6. Photoacoustic method for characterizing a plant according to any one of claims 1 to 5, characterized in that the measurement is demodulated to identify a phase and a modulus.

7. Photoacoustic method for characterizing a plant according to any one of claims 1 to 6, characterized in that the plant (10) is alive.

8. Photoacoustic equipment for characterizing a plant, the equipment comprising means (100) for applying laser radiation, a photoacoustic cavity (155) configured so that the laser radiation can be applied through the cavity, and means for recording by a microphone (175) sound waves appearing by photothermal effect in the photoacoustic cavity (155), characterized in that the equipment further comprises a support comprising the cavity, the support also comprising an insertion space for a section of plant stem, root or petiole and sealing means (190, 192) for the cavity at each of the two ends of said section.

9. Photoacoustic equipment for characterizing a plant according to claim 8, characterized in that the support is composed of a set of two opposing jaws (151, 152) forming a vise and each provided with a circular sealing joint to form with the opposite joint a set of two opposing passages on either side of the cavity with sealing for the placement of the stem, root or petiole.

10. Photoacoustic equipment for characterizing a plant according to claim 8 or claim 9, characterized in that the means (100) for applying laser radiation comprise a tunable laser, or several optically coupled monochromatic lasers.

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