IDENTIFICATION, DIFFERENTIATION, AND PHYLOGENETIC MAPPING METHOD OF INDONESIAN NATURAL STRAINS OF RABIES VIRUS USING AMPLIFICATION OF THE N GENE FRAGMENT (1047 BP) AND G GENE (1053 BP)

Abstract

This invention concerns a method for identification, differentiation, and phylogenetic mapping of natural Indonesian rabies virus strains based on molecular analysis. This method includes the stages of extracting rabies virus RNA from biological samples, reverse transcription of RNA into cDNA, amplification of a nucleoprotein gene fragment with a length of 1047 base pairs and a glycoprotein gene fragment with a length of 1053 base pairs using polymerase chain reaction techniques, visualization of the amplification results, nucleotide sequencing, and homology and phylogenetic analysis. The use of two gene targets with specific fragment lengths allows for accurate identification of rabies viruses, differentiation between isolates based on geographic regions, and mapping of kinship relationships and the origins of rabies viruses in Indonesia.This method can be applied in molecular diagnostic activities, epidemiological surveillance, and tracing the spread of the rabies virus, and provides increased accuracy and efficiency compared to previous identification methods that only use one gene target.