Composition for improving skin texture

Abstract

To provide a composition capable of improving a skin texture.SOLUTION: A composition contains (A) procyanidin B1 and (B) procyanidin B3, where the mass ratio of the component (A) to the component (B) [(A) / (B)] is 1 or more. The composition may further contain (C) catechin.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a composition for improving skin quality. [Background technology]

[0002] Skin conditions such as dry skin, wrinkles, sagging skin, loss of elasticity, dullness, and dryness are factors that impair the beauty of the skin and are a source of concern for women who are concerned about their beauty. For this reason, cosmetics, medicines, and foods that have skin quality improving effects have been developed with the aim of improving skin quality.

[0003] For example, known compositions include a cosmetic composition for moisturizing the skin that contains bentonite as an active ingredient (Patent Document 1), a skin quality improving composition that contains Lactobacillus rhamnosus cells or an extract of the cells as an active ingredient (Patent Document 2), and a skin quality improving composition that contains black turmeric extract as an active ingredient (Patent Document 3). However, the compositions developed so far are not necessarily effective enough, and there is a demand for the development of a new composition that has excellent skin quality improving properties. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Special Publication No. 2022-543578 [Patent Document 2] JP 2022-182870 A [Patent Document 3] Patent Publication No. 2021-023279 Summary of the Invention [Problem to be solved by the invention]

[0005] An object of the present invention is to provide a composition capable of improving skin quality.

[0006] The present inventors have found that an excellent skin quality improving effect can be obtained by adjusting procyanidin B1 (hereinafter, also referred to as "PB1") and procyanidin B3 (hereinafter, also referred to as "PB3") to a specific ratio. Furthermore, they have found that an excellent skin quality improving effect can be obtained by containing catechin in addition to procyanidin B1 and procyanidin B3. The present invention has been completed based on such findings.

[0007] That is, the present invention is as follows. [1] A composition for improving skin quality, comprising (A) procyanidin B1 and (B) procyanidin B3, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or greater. [2] A composition for improving skin quality, comprising (A) procyanidin B1, (B) procyanidin B3, and (C) catechin, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more. [3] A composition for improving skin quality, comprising (A) procyanidin B1, (B) procyanidin B3, and (C) catechin, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more, and the mass ratio of component (A) to component (C) [(A) / (C)] is 0.5 or more. [4] A composition for improving skin quality, comprising (A) procyanidin B1 and (B) procyanidin B3, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more and 10 or less. [5] A skin quality improving composition comprising (A) procyanidin B1, (B) procyanidin B3 and (C) catechin, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more and 10 or less. [6] A composition for improving skin quality, comprising (A) procyanidin B1, (B) procyanidin B3, and (C) catechin, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more and 10 or less, and the mass ratio of component (A) to component (C) [(A) / (C)] is 0.5 or more and 10 or less. [7] The composition for improving skin quality according to any one of [1] to [6], wherein the improvement in skin quality is improvement in skin brightness, skin moisture, or skin elasticity. Effect of the Invention

[0008] The composition of the present invention has excellent effects of improving skin brightness, skin moisture, and skin elasticity, and therefore exhibits excellent effects of improving skin quality. [Brief description of the drawings]

[0009] [Figure 1] FIG. 1 shows the epidermal keratinocyte activating activity in Examples and Comparative Examples. [Diagram 2] FIG. 2 shows the activating activity of skin fibroblasts in the examples and comparative examples. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0010] The skin quality improving composition of the present invention is characterized by containing (A) procyanidin B1 and (B) procyanidin B3 in a specific ratio. Furthermore, the composition of the present invention may contain (C) catechin. Each component and the composition of the present invention will be described below. Note that the configurations described below do not limit the present invention, and various modifications can be made within the scope of the gist of the present invention.

[0011] The composition of the present invention exhibits a skin quality improving effect, and therefore can be used as a skin quality improving composition.

[0012] Each component contained in the composition of the present invention will be described below. [(A) Procyanidin B1] (A) Procyanidin B1 is a dimer formed by the C4-C8 bond between (-) epicatechin and (+) catechin, and has the chemical structure shown in formula (1) below.

[0013] [ka]

[0014] The (A) procyanidin B1 of the present invention can be synthetic or derived from plants such as apple, grape, or pine, with plant-derived materials being preferred from the standpoint of safety during use, and grape or pine being more preferred as they are rich in procyanidin B1, with pine being particularly preferred.

[0015] The processing method of the plant-derived material is not particularly limited, and processed products such as crushed products, squeezed juice, and extracts can be used. Examples of crushed products include powder and granules. The squeezed juice and extract may be in liquid form, but can also be used as a paste or dry powder. When making it into a paste or dry powder, it may be produced by using only the material itself, or may be processed together with an excipient. The extract can be obtained by extraction using a suitable solvent, and examples of the solvent that can be used include water, ethanol, and aqueous ethanol. In the present invention, it is particularly preferable to use an extract because it can contain a large amount of procyanidin B1.

[0016] The content of procyanidin B1 in the composition of the present invention can be analyzed by HPLC. For example, it can be measured by an HPLC analyzer equipped with an ultraviolet absorption detector, and the analytical column is L-Column ODS 3μm (4.6×250mm) manufactured by Chemicals Evaluation and Research Institute, Inc., Japan, and the mobile phase is a 0.1M aqueous acetic acid solution (mobile phase A) and a 0.1M acetic acid acetonitrile solution (mobile phase B). The column temperature is 40°C and the flow rate is 1.0mL / min. The gradient conditions can be as follows.

[0017] [Table A]

[0018] [(B) Procyanidin B3] (B) Procyanidin B3 is a dimer formed by the C4-C8 bond between (+) catechin and (+) catechin, and has the chemical structure shown in formula (2) below.

[0019] [ka]

[0020] The (B) procyanidin B3 of the present invention can be synthetic or derived from plants such as apple, grape, or pine, with plant-derived materials being preferred from the standpoint of safety during use, and grape or pine being more preferred since they are rich in procyanidin B3, with pine being particularly preferred.

[0021] The processing method of the plant-derived material is not particularly limited, and processed products such as crushed products, squeezed juice, and extracts can be used. Examples of crushed products include powder and granules. The squeezed juice and extract may be in liquid form, but can also be used as a paste or dry powder. When making it into a paste or dry powder, it may be produced by using only the material itself, or may be processed together with an excipient. The extract can be obtained by extraction using a suitable solvent, and the solvent may be, for example, water, ethanol, or aqueous ethanol. In the present invention, it is particularly preferable to use an extract because it can contain a large amount of procyanidin B3.

[0022] The content of procyanidin B3 in the composition of the present invention can be analyzed by HPLC. For example, it can be measured by an HPLC analyzer equipped with an ultraviolet absorption detector, and the analytical column is L-Column ODS 3μm (4.6×250mm) manufactured by Chemicals Evaluation and Research Institute, Inc., Japan, and the mobile phase is 0.1M acetic acid aqueous solution (mobile phase A) and 0.1M acetic acid acetonitrile solution (mobile phase B). The column temperature is 40°C and the flow rate is 1.0mL / min. The gradient conditions can be as follows.

[0023] [Table B]

[0024] [(C) Catechin] The composition of the present invention may contain (C) catechin. Catechin is a compound represented by the chemical formula C15H14O6, and the catechin in the present invention is (+)-catechin represented by the following chemical formula (3) or (-)-catechin represented by the chemical formula (4). As the catechin, either (+)-catechin or (-)-catechin may be used, or the two types may be used in combination, but it is preferable to use the two types in combination in order to obtain a higher effect.

[0025] [ka]

[0026] The catechin (C) of the present invention can be synthetic or derived from plants such as tea or pine, with plant-derived substances being preferred from the standpoint of safety during use, and tea or pine being more preferred since they are rich in catechins, with pine being particularly preferred.

[0027] The processing method of the plant-derived material is not particularly limited, and processed products such as crushed products, squeezed juice, and extracts can be used. Examples of crushed products include powder and granules. The squeezed juice and extract may be in liquid form, but can also be used as a paste or dry powder. When making it into a paste or dry powder, it may be produced using only the material itself, or may be processed together with an excipient. The extract can be obtained by extraction using a suitable solvent, and examples of the solvent that can be used include water, ethanol, and aqueous ethanol. In the present invention, it is particularly preferable to use an extract because it can contain a large amount of catechins.

[0028] The content of catechin in the composition of the present invention can be analyzed by HPLC. For example, it can be measured by an HPLC analyzer equipped with an ultraviolet absorption detector, and the analytical column is L-Column ODS 3μm (4.6×250mm) manufactured by Chemicals Evaluation and Research Institute, Inc., Japan, and the mobile phase is 0.1M acetic acid aqueous solution (mobile phase A) and 0.1M acetic acid acetonitrile solution (mobile phase B). The column temperature is 40°C and the flow rate is 1.0mL / min. The gradient conditions can be as follows.

[0029] [Table C]

[0030] The composition of the present invention can be used, for example, as a pharmaceutical product (including quasi-drugs), a cosmetic product, or a so-called health food such as a functional food whose efficacy has been approved by a designated organization, such as a food for specified health uses, a food with nutrient function claims, or a food with functional claims.

[0031] The composition of the present invention has an epidermal keratinocyte activating effect and a skin fibroblast activating effect, as described in the examples below. By activating epidermal keratinocytes, skin turnover is promoted, melanin in the skin is discharged, and skin brightness can be improved more effectively. In addition, by activating skin fibroblasts, production of collagen and hyaluronic acid is promoted, and the skin barrier function can be more effectively enhanced, skin moisture can be improved, and skin elasticity can be improved. Therefore, the skin quality improving composition of the present invention can be used as a composition for activating epidermal keratinocytes, a composition for activating fibroblasts, a composition for promoting skin turnover, a composition for improving skin brightness, a composition for producing collagen, a composition for promoting hyaluronic acid production, a composition for improving skin barrier function, a composition for improving skin moisture, and a composition for improving skin elasticity. The skin includes the scalp and lips.

[0032] The composition of the present invention is not particularly limited as long as it contains procyanidin B1 and procyanidin B3, and optionally contains catechin, and can be distinguished from other products in that it exhibits the function of improving skin quality. For example, the scope of the present invention includes products that display the function of improving skin quality on the main body, packaging, instructions, or promotional materials (advertising media) of the product of the present invention.

[0033] In addition, when the composition of the present invention does not contain catechin, it may be one in which procyanidin B1 or / and procyanidin B3 is indicated as an active ingredient, but is not limited to one in which procyanidin B1 and procyanidin B3 are indicated as active ingredients. When the composition of the present invention contains catechin, it may be one in which procyanidin B1 or / and procyanidin B3 or / and catechin is indicated as an active ingredient, but is not limited to one in which procyanidin B1, procyanidin B3, and catechin are indicated as active ingredients. For example, it may be one in which no active ingredients are indicated. In addition, even if it is a general food, if it is manufactured and sold with the function and use suggested, it is included in the scope of the present invention. For example, there is a food sold by posting a testimonial that refers to the function and use as a personal impression of the person who ingested it on a website or the like.

[0034] Specifically, examples of compositions for improving skin quality include those labeled with claims such as "improving skin wrinkles," "improving skin sagging," "maintaining skin barrier function," "increasing skin brightness," "maintaining skin elasticity," "promoting skin turnover," "increasing collagen production," "proliferation of fibroblasts," "improving makeup application," "providing a skin barrier," "protecting the skin," "enhancing skin barrier function," "enhancing skin moisturizing power," "for beautiful skin," "beautiful skin," "for youthful skin," "whitening," "beauty," "making the skin appear younger," "suppressing skin itching," "increasing skin moisture," "improving skin moisture," "increasing skin moisture," "improving the scalp environment," "improving scalp elasticity," "improving scalp moisture," "increasing lip moisture," "for those concerned with blemishes," "for those concerned with wrinkles," "for skin that can withstand stress," "for skin that can withstand ultraviolet rays," "good for the skin," "reducing the amount of moisture evaporation from the skin," "preventing dry skin," "for those concerned with firmness," "for those concerned with dullness," "improving skin transparency," and "for transparent skin."

[0035] The composition of the present invention can be used for oral or external use. When the composition of the present invention is used for oral use, its form may be, for example, powder, granule, tablet, liquid, gel, paste, capsule such as hard capsule or soft capsule, caplet, tablet, gel, jelly, gummy, wafer, biscuit, cookie, cake, chewable, syrup, stick, etc. Among these, granule, tablet, capsule, and liquid are preferred from the viewpoint of easy intake, and granule, tablet, and capsule are more preferred. Here, granule refers to powder granulated, and may be directly drunk or dissolved in liquid such as water and drunk. In addition, gel refers to a state containing water and a gelling agent and having viscosity or elasticity.

[0036] When the composition of the present invention is used for external application, its form may be, for example, an ointment, cream, gel, lotion, emulsion, pack, poultice, etc. Among these, from the viewpoint of ease of application, cream, gel, lotion, emulsion, and pack are preferred, and cream, gel, lotion, and emulsion are more preferred.

[0037] The mass ratio of (A) procyanidin B1 to (B) procyanidin B3 in the composition of the present invention [(A) / (B)] may be 1 or more, preferably 1.5 or more, more preferably 2 or more, and particularly preferably 2.5 or more. There is no particular upper limit, but it is preferably 10 or less, more preferably 5 or less, and particularly preferably 3.5 or less. By using these mass ratios, a higher effect can be obtained.

[0038] The mass ratio of (A) procyanidin B1 to (C) catechin in the composition of the present invention [(A) / (C)] is not particularly limited, but is preferably 0.1 or more, more preferably 0.5 or more, and particularly preferably 0.8 or more. The upper limit is not particularly limited, but is preferably 10 or less, more preferably 7 or less, and particularly preferably 3 or less. By using these mass ratios, a higher effect can be obtained.

[0039] The mass ratio of the sum of (A) procyanidin B1 and (B) procyanidin B3 to (C) catechin in the composition of the present invention [((A)+(B)) / (C)] is not particularly limited, but is preferably 0.2 or more, more preferably 0.5 or more, and particularly preferably 1 or more. The upper limit is not particularly limited, but is preferably 10 or less, more preferably 7 or less, and particularly preferably 3.5 or less. By using these mass ratios, a higher effect can be obtained.

[0040] The total content of (A) procyanidin B1 and (B) procyanidin B3 in the composition of the present invention may be appropriately determined within the range in which the effect is exhibited, regardless of whether or not catechin is contained. For example, the composition of the present invention may contain 0.00001 to 30% by mass, preferably 0.0001 to 20% by mass, and more preferably 0.001 to 10% by mass, calculated as a dry mass.

[0041] When the composition of the present invention is an oral composition, there is no particular limit to the amount of intake, but in order to obtain a higher effect, it is preferable that the total intake of (A) procyanidin B1 and (B) procyanidin B3 is 0.1 mg or more per day for an adult, more preferably 1 mg or more, and even more preferably 2 mg or more. The upper limit is, for example, 100 mg, preferably 50 mg, and more preferably 20 mg.

[0042] When the composition of the present invention is an oral composition, the total daily intake of (A) procyanidin B1 and (B) procyanidin B3 may be appropriately designed to be the above intake amount, and may be in a form in which the above intake amount is taken in one dose, or in a form in which the above intake amount is taken in multiple doses. That is, for example, it can be stored in one container or in multiple containers (2 to 4) as a daily amount. Furthermore, when the composition of the present invention is a solid formulation (powder, granule, tablet, capsule, chewable, etc.), the daily intake amount of the composition of the present invention is preferably 0.05 to 50 g, more preferably 0.1 to 10 g, and even more preferably 0.2 to 5 g. Furthermore, when the composition of the present invention is a liquid formulation (liquid, gel, paste, etc.), the daily intake amount is preferably 10 to 1500 g, more preferably 30 to 1000 g, and even more preferably 50 to 750 g.

[0043] When the composition of the present invention is an external preparation, the amount to be used is not particularly limited and can be appropriately selected in consideration of various factors such as the age, weight, constitution, etc. of the individual to be used. In addition, it can be used once or several times a day in divided doses.

[0044] The composition of the present invention can be produced by known methods by adding ingredients other than (A) procyanidin B1, (B) procyanidin B3, and (C) catechin, as necessary. EXAMPLES

[0045] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0046] <Test 1: Evaluation of epidermal keratinocyte activation> Epidermal keratinocyte activation was evaluated.

[0047] [Test substance] The test substances used were (A) procyanidin B1 reagent (manufactured by ChemFaces) as procyanidin B1, (B) procyanidin B3 reagent (manufactured by ChemFaces) as procyanidin B3, and (C) catechin reagent (manufactured by Nagara Science) as catechin.

[0048] [cell] Human epidermal keratinocytes (NHEK) were used as epidermal keratinocytes.

[0049] [culture] In a 37℃, 5% CO2 incubator, 75 cm 2 Human epidermal keratinocytes were cultured in a keratinocyte growth medium (Takara Bio) flask. The cells were then suspended by lactase treatment and placed in a 75 cm 2 From the flask, place 8 x 10 cells in each well of a 96-well plate. 3 The cells were seeded at a density of 100 cells / well and precultured for 24 hours in a 5% by volume CO2 incubator at 37°C.

[0050] [Epidermal keratinocyte proliferation test] After removing the medium from each well, 100 μL / well of medium containing a test substance prepared to a predetermined concentration was added, and the cells were cultured in a 37°C, 5% by volume CO2 incubator for 24 hours. As the test substance, the compositions of Examples 1 to 9 and Comparative Examples 1 to 5 shown in Table 1 below were used. The medium containing the test substance was prepared so that the total concentration of each component of the composition in the culture medium was 1 μg / mL. As a control, a culture medium containing no test substance was added.

[0051] [Table 1]

[0052] After 24 hours of culture, the medium was removed, and 150 μL of Cell Counting Kit-8 (Dojindo Chemical Industries, Ltd.) diluted 30-fold with serum-free DMEM was added to each well. The plate was placed in a 37°C, 5% by volume CO2 incubator to allow for adequate color development, and the absorbance at 450 nm was measured. Based on the data obtained, the % of control (relative number of live cells with the number of live cells in the control taken as 100%) was calculated. The results are shown in Figure 1. The absorbance ratio and the ratio of human epidermal keratinocyte counts are directly proportional to each other, so the absorbance ratio indicates the ratio of human epidermal keratinocyte counts. % of control=(Data sample-Data blank) / (Data control-Data blank)×100

[0053] [Test Results] 1, the group (Examples 1 to 9) in which procyanidin B1 (A) and procyanidin B3 (B) were added and the mass ratio of component (A) to component (B) [(A) / (B)] was 1 or more exhibited epidermal keratinocyte activation, compared to the group (Comparative Examples 1 to 4) in which procyanidin B1 (A) and procyanidin B3 (B) were added and the mass ratio of component (A) to component (B) [(A) / (B)] was less than 1, and the group (Comparative Example 5) in which catechin (C) was added alone. Among Examples 1 to 9, the group (Examples 4 to 9) in which catechin (C) was added exhibited superior epidermal keratinocyte activation, compared to the group (Examples 1 to 3) in which catechin (C) was not added. Furthermore, among them, the group (Examples 4 to 8) in which the mass ratio [(A) / (C)] of component (A) to component (C) is 0.5 or more was found to have particularly excellent epidermal keratinocyte activation, compared to the group (Example 9) in which the mass ratio [(A) / (C)] of component (A) to component (C) is less than 0.5. Therefore, by combining (A) procyanidin B1 and (B) procyanidin B3 and setting the mass ratio [(A) / (B)] of component (A) to component (B) to 1 or more, epidermal keratinocyte activation is exerted, so that the brightness of the skin is improved and a superior skin quality improvement effect can be obtained. In addition, by combining (C) catechin, a more excellent epidermal keratinocyte activation is exerted, so that a more excellent skin quality improvement effect can be obtained. Furthermore, by setting the mass ratio [(A) / (C)] of component (A) to component (C) to 0.5 or more, a particularly excellent epidermal keratinocyte activation is exerted, so that a particularly excellent skin quality improvement effect can be obtained.

[0054] <Test 2: Evaluation of skin fibroblast activation> Skin fibroblast activation was evaluated.

[0055] [Test substance] As in Test 1, the test substances used were (A) procyanidin B1 reagent (manufactured by ChemFaces) as procyanidin B1, (B) procyanidin B3 reagent (manufactured by ChemFaces) as procyanidin B3, and (C) catechin reagent (manufactured by Nagara Science) as catechin.

[0056] [cell] Human dermal fibroblasts (NHDFs) were used as skin fibroblasts.

[0057] [culture] In a 37℃, 5% CO2 incubator, 75 cm 2 Human dermal fibroblasts were cultured in 10% FBS-DMEM medium using a flask. The cells were then suspended by trypsinization and placed in a 75 cm 2 From the flask to each well of a 96-well plate, 5 x 10 3 The cells were seeded at a density of 100 cells / well and precultured for 24 hours in a 5% by volume CO2 incubator at 37°C.

[0058] [Skin fibroblast proliferation test] After removing the medium from each well, 100 μL / well of a medium containing a test substance prepared to a predetermined concentration was added, and the cells were cultured in a 37°C, 5% by volume CO2 incubator for 24 hours. After removing the medium from each well, 100 μL / well of a medium containing a test substance prepared to a predetermined concentration was added, and the cells were cultured in a 37°C, 5% by volume CO2 incubator for 24 hours. As test substances, the compositions of Examples 1 to 9 and Comparative Examples 1 to 5 shown in Table 1 were used. The test substance-containing medium was prepared so that the total concentration of each component of the composition in the culture medium was 1 μg / mL. In addition, a culture medium not containing a test substance was added as a control.

[0059] After 24 hours of culture, the medium was removed, and 150 μL of Cell Counting Kit-8 (Dojindo Chemical Industries) diluted 30-fold with serum-free DMEM was added to each well. The plate was left to stand in a 37°C, 5% by volume CO2 incubator to allow for adequate color development, and the absorbance at 450 nm was measured. Based on the data obtained, the % of control (relative number of live cells with the number of live cells in the control taken as 100%) was calculated. The results are shown in Figure 2. The absorbance ratio and the ratio of human dermal fibroblast numbers are directly proportional to each other, so the absorbance ratio indicates the ratio of human dermal fibroblast numbers. % of control=(Data sample-Data blank) / (Data control-Data blank)×100

[0060] [Test Results] 2, the group (Examples 1 to 9) to which procyanidin B1 (A) and procyanidin B3 (B) were added and the mass ratio of component (A) to component (B) [(A) / (B)] was 1 or more exhibited skin fibroblast activation, compared to the group (Comparative Examples 1 to 4) to which procyanidin B1 (A) and procyanidin B3 (B) were added and the mass ratio of component (A) to component (B) [(A) / (B)] was less than 1, and the group (Comparative Example 5) to which catechin (C) was added alone. Among Examples 1 to 9, the group (Examples 4 to 9) to which catechin (C) was added exhibited superior skin fibroblast activation, compared to the group (Examples 1 to 3) to which catechin (C) was not added. Furthermore, among them, the group (Examples 4 to 8) in which the mass ratio [(A) / (C)] of component (A) to component (C) is 0.5 or more was found to have particularly excellent skin fibroblast activation, compared to the group (Example 9) in which the mass ratio [(A) / (C)] of component (A) to component (C) is less than 0.5. Therefore, by combining (A) procyanidin B1 and (B) procyanidin B3 and setting the mass ratio [(A) / (B)] of component (A) to component (B) to 1 or more, skin fibroblast activation is exerted, thereby improving skin moisture and skin elasticity, and obtaining an excellent skin quality improvement effect. In addition, by combining (C) catechin, more excellent skin fibroblast activation is exerted, thereby obtaining an even more excellent skin quality improvement effect. Furthermore, by setting the mass ratio [(A) / (C)] of component (A) to component (C) to 0.5 or more, particularly excellent skin fibroblast activation is exerted, thereby obtaining an especially excellent skin quality improvement effect.

[0061] Based on the results of the Examples, Production Examples of the present invention are given below.

[0062] [Production Example 1-3: Granules 1] Granules containing (A) procyanidin B1 and (B) procyanidin B3 were produced according to the composition shown in Table 2. The granules described in Production Examples 1-3 may be taken at 3 g per day, and may be taken by dissolving in 100 ml of a solvent such as water, or may be taken as is without dissolving. All of the granules in Production Examples 1-3 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0063] [Table 2]

[0064] [Production Example 4-6: Granules 2] Granules containing (A) procyanidin B1 and (B) procyanidin B3 were produced according to the formulation in Table 3. The granules described in Production Examples 4-6 can be taken at a dose of 25 g per day by dissolving in 200 ml of water or the like. All of the granules in Production Examples 4-6 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0065] [Table 3]

[0066] [Production Example 7-9: Tablets] Tablets containing (A) procyanidin B1 and (B) procyanidin B3 were produced according to the formulation in Table 4. The tablets were produced with a tablet diameter of 8 mmφ, tablet thickness of 4.5 mm, weight of 250 mg, and hardness of 5 kgf or more. One to four tablets of the tablets described in Production Example 7-9 may be taken per day, and can be taken with 100 ml of water or the like. All tablets of Production Examples 7-9 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0067] [Table 4]

[0068] [Production Example 10-12: Hard Capsule] Hard capsules containing (A) procyanidin B1 and (B) procyanidin B3 were produced by encapsulating the ingredients in the composition of Table 5 with a coating containing gelatin or hydroxypropyl cellulose. Each hard capsule was produced at 300 mg. One to four capsules may be taken per day, and may be taken with 100 ml of water or the like. In addition, all of the hard capsules of Production Examples 10 to 12 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0069] [Table 5]

[0070] [Production Example 13-15: Soft Capsule] The ingredients in the formulation of Table 6 were encapsulated with a coating containing gelatin to produce soft capsules containing (A) procyanidin B1 and (B) procyanidin B3. Each soft capsule was produced at 300 mg. One to four capsules may be taken per day, and can be taken with 100 ml of water or the like. In addition, all of the soft capsules of Production Examples 13-15 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0071] [Table 6]

[0072] [Manufacturing example 16-18: PET beverage] PET beverages containing (A) procyanidin B1 and (B) procyanidin B3 were produced according to the formulation in Table 7. The PET beverages were produced in 500ml bottles. One bottle should be consumed per day. In addition, all of the PET beverages of Production Examples 16-18 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0073] [Table 7]

[0074] [Production Example 19-21: Chocolate] Chocolate containing (A) procyanidin B1 and (B) procyanidin B3 was produced according to the composition shown in Table 8. It is sufficient to take 30g per day. In addition, all of the chocolates of Production Examples 19 to 21 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0075] [Table 8]

[0076] [Production Example 22-24: Jelly] A jelly containing (A) procyanidin B1 and (B) procyanidin B3 was produced according to the composition shown in Table 9. It is sufficient to take 150 g per day. In addition, all of the jellies of Production Examples 22-24 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0077] [Table 9]

[0078] [Production Example 25-27: Gummies] Gummies containing (A) procyanidin B1 and (B) procyanidin B3 were manufactured according to the composition shown in Table 10. 4g should be taken per day. In addition, all of the gummies of Manufacturing Examples 25-27 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0079] [Table 10]

[0080] [Production Example 28-30: Yogurt] Yogurt containing (A) procyanidin B1 and (B) procyanidin B3 was produced according to the composition shown in Table 11. It is sufficient to take 150g per day. In addition, all of the yogurts of Production Examples 28 to 30 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0081] [Table 11]

[0082] [Production Example 31-33: Lotion] A lotion containing (A) procyanidin B1 and (B) procyanidin B3 was prepared according to the formulation in Table 12. All of the lotions of Preparation Examples 31 to 33 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0083] [Table 12]

[0084] [Production Example 34-36: Emulsion] Milk lotions containing (A) procyanidin B1 and (B) procyanidin B3 were produced according to the formulation in Table 13. All of the milk lotions of Production Examples 34 to 36 were excellent in improving skin brightness, skin moisture, and skin elasticity, and were effective in improving skin quality.

[0085] [Table 13]

[0086] [Manufacturing Example 37-39: Cosmetic cream] Cosmetic creams containing (A) procyanidin B1 and (B) procyanidin B3 were produced according to the formulation in Table 14. All of the cosmetic creams of Production Examples 37 to 33 are excellent in improving skin brightness, skin moisture, and skin elasticity, and are effective in improving skin quality.

[0087] [Table 14] [Industrial Applicability]

[0088] The composition of the present invention is industrially useful since it can improve skin quality and can be used as health foods, cosmetics, etc.

Claims

1. A skin quality improving composition containing (A) procyanidin B1 and (B) procyanidin B3, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more.

2. A skin quality improving composition containing (A) procyanidin B1, (B) procyanidin B3, and (C) catechin, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more.

3. A skin quality improving composition containing (A) procyanidin B1, (B) procyanidin B3 and (C) catechin, wherein the mass ratio of component (A) to component (B) [(A) / (B)] is 1 or more, and the mass ratio of component (A) to component (C) [(A) / (C)] is 0.5 or more.

4. 4. The composition for improving skin quality according to claim 1, wherein the improvement of skin quality is improvement of skin brightness, skin moisture, or skin elasticity.

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