Method for amplifying DNA from formalin-fixed sample
JP2025000190A5Pending Publication Date: 2026-06-03KANSAI MEDICAL UNIVERSITY
Patent Information
- Authority / Receiving Office
- JP · JP
- Patent Type
- Applications
- Current Assignee / Owner
- KANSAI MEDICAL UNIVERSITY
- Filing Date
- 2023-06-19
- Publication Date
- 2026-06-03
AI Technical Summary
Technical Problem
Formalin-fixed samples, especially those older than 3 years, are unsuitable for genetic testing due to DNA fragmentation, which inhibits amplification and sequencing, leading to poor DNA quality and restricted testing timelines.
Method used
A method involving DNA extraction from formalin-fixed samples, followed by removal of fragmented DNA using a silica-based solid phase material, and subsequent amplification of target DNA, preferably via PCR, to enhance DNA integrity for genetic analysis.
Benefits of technology
Enables successful amplification and sequencing of DNA from formalin-fixed samples stored for over 3 years, allowing genetic testing and identification of diagnostic markers and disease-causing genes, even in forensic samples.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure 00000010_0000 
Figure 00000010_0001
Abstract
To provide a method for amplifying the target DNA from a formalin-fixed sample.SOLUTION: The present invention provides a method for amplifying DNA, comprising the following steps of: (1) extracting DNA from a formalin-fixed sample, (2) removing fragmented DNA from a solution containing the extracted DNA, and (3) amplifying the target DNA in the solution derived from the step (2).SELECTED DRAWING: None
Need to check novelty before this filing date? Find Prior Art