Topical skin preparation

Abstract

To provide a topical skin preparation made from natural products with superior biological safety, incorporating active ingredients with an effect of sustainedly enhancing moisture sensation, transparency, and gloss, and an effect of activating oxidized ascorbic acid reductase in vivo.SOLUTION: The present invention provides a topical skin preparation comprising an extract from one or more plants selected from Citrus tachibana and Citrus unshiu×Citrus noblis as an active ingredient.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to an external skin preparation containing, as an active ingredient, an extract of one or more plants belonging to the family Rutaceae. [Background technology]

[0002] Skin aging or disorders is a phenomenon that occurs due to a complex intertwining of internal factors such as inactivation of cell proliferation and differentiation, decreased hormone secretion, and quantitative decrease in extracellular matrix components with aging, and external factors such as cell and tissue damage caused by active oxygen induced by sunlight (ultraviolet rays), or inflammation. Examples of skin aging or disorders include wrinkles, sagging, loss of elasticity, dryness, decreased barrier function, pigmentation, dullness, etc., and wrinkle improvement agents, moisturizers, age spot prevention agents, etc. have been proposed according to each skin condition.

[0003] Conventionally, antioxidants such as vitamins A and E and moisturizers (e.g., amino acids, organic acids, hyaluronic acid or its salts, chondroitin, heparinoids, etc.) have been proposed as ingredients for preventing or improving skin aging or disorders, but there are problems with stability, efficacy, and safety when blended into skin external preparations. In addition, the use of ingredients derived from natural products such as plants has also been proposed, but there are problems with efficacy. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 07-118132 [Patent Document 2] Japanese Patent Publication No. 08-295621 [Patent Document 3] Patent Publication No. 2000-096050 [Patent Document 4] JP2001-114634A DISCLOSURE OF THEINVENTION [Problem to be solved by the invention]

[0005] In view of the problems of the prior art, the present inventors have conducted extensive research to find new, highly effective plant-derived components. As a result, they have found that one or more extracts of Citrus tachibana and Citrus unshiu × Citrus noblis, which belong to the Rutaceae family, have the effect of continuously improving the moisturizing, translucency, and luster of the skin, and the effect of activating an enzyme that reduces "oxidized ascorbic acid" generated by oxidation in the body.

[0006] The use of Rutaceae plants in topical skin preparations has been known in the past, for example, from Patent Documents 1 to 4. However, it was not known that they have the effect of continuously improving the moisturizing, transparency, and luster of the skin, or of activating the enzyme that reduces oxidized ascorbic acid. [Means for solving the problem]

[0007] The present invention is an external skin preparation that contains an extract of either one or two of Citrus tachibana and Citrus unshiu × Citrus noblis as active ingredients, and that continuously improves the skin's moisturizing, clarity and luster. The present invention also relates to an activator of oxidized ascorbic acid reductase, which comprises an extract of Calamandarin (Citrus unshiu × Citrus noblis) as an active ingredient. Effect of the Invention

[0008] According to the present invention, by using an extract of any one or two of Citrus tachibana and Citrus unshiu × Citrus noblis as an active ingredient, it is possible to provide an external skin preparation having the effect of continuously improving moisturizing feeling, transparency and luster. Furthermore, according to the present invention, by using an extract of Calamandarin (Citrus unshiu × Citrus noblis) as an active ingredient, it is possible to activate the enzyme that reduces oxidized ascorbic acid, returning it to reduced ascorbic acid that exerts antioxidant properties, thereby suppressing the effects of active oxygen and the like on the skin and enhancing the effects of ascorbic acid (including derivatives) applied to the skin through external application. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0009] The Rutaceae plant used in the present invention is Tachibana (Citrus tachibana or Citrus aurantium var. tachibana) and Calamandarin (Citrus unshiu × Citrus noblis). Although the variety of Tachibana is not limited, Nippon Tachibana (also known as Yamato Tachibana) is more preferable.

[0010] Examples of parts of Tachibana or Calamansia that can be used include the fruit, the peel, and the flesh.

[0011] The preparation of the extract is carried out by first washing the part to be used for extraction with water if necessary to remove foreign matter, leaving it as it is or drying it, and then cutting or crushing it as necessary, and contacting it with an extraction solvent to carry out extraction. The extraction can be carried out by contacting it with an extraction solvent according to a conventional method such as the immersion method, but it is also possible to use a supercritical extraction method other than the immersion method.

[0012] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination of two or more.

[0013] Among the above-mentioned extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoint of the effectiveness of the obtained extract, furthermore, skin irritation, and also from the viewpoint of wide applicability to skin external preparations (cosmetics, quasi-drugs, external medicines, etc.).Preferred examples of the hydrophilic solvent include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol, glycerin) alone, or a mixed solvent of water and lower alcohols (particularly ethanol), or a mixed solvent of water and polyhydric alcohols (particularly 1,3-butylene glycol, glycerin), etc.

[0014] When a mixed solvent is used, the mixing ratio, for example, of water and a lower alcohol or a polyhydric alcohol, is preferably 1:5 to 25:1 in terms of volume ratio (the same applies below).

[0015] In preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, an alkalinity regulator such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity regulator such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid may be added to the extraction solvent to adjust the pH to a desired level.

[0016] The extraction conditions such as extraction temperature and extraction time vary depending on the type and pH of the solvent used. For example, when water alone or a mixture of water and a lower alcohol or a polyhydric alcohol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, and the extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week), and more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0017] In order to remove impurities and improve stability, the extract of the present invention may be subjected to one or a combination of two or more of activated carbon treatment, ion exchange resin treatment, synthetic adsorbent treatment, silica gel, and recrystallization treatment to remove impurities other than the active ingredients.

[0018] The extract prepared as described above may be used as an ingredient in a skin external preparation as it is after adjusting the pH to 3 to 8, or may be used at a desired concentration by concentrating under reduced pressure, etc. The extract may also be dried by a conventional method such as spray drying.

[0019] Examples of topical skin preparations (cosmetics, quasi-drugs, topical medicines) containing the extract of the present invention include, but are not limited to, milky lotions, creams, lotions, essences, packs, lipsticks, foundations, sheet masks, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, soaps and other cleansing cosmetics, hair growth products, and bath additives.

[0020] When the extract of the present invention is incorporated into an external skin preparation, the amount of the extract is generally in the range of 0.00001 to 5.0% by weight in terms of the solid content of each extract.

[0021] When the extract of the present invention is incorporated into topical skin preparations (such as cosmetics, quasi-drugs, and topical pharmaceuticals), ingredients used in topical skin preparations, such as oily ingredients, surfactants (synthetic and natural), moisturizers, thickeners, emulsifiers or emulsification aids, preservatives and germicides, powder ingredients, UV absorbers, antioxidants, sequestering agents, pigments, fragrances, anti-wrinkle agents, pigmentation prevention agents, and other physiologically active ingredients, can be appropriately incorporated as necessary.

[0022] Examples of oily components include plant-derived oils such as olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, lemon peel oil, camphor bark oil, mandarin orange oil, cardamom oil, and orange oil; vitamin A oil; animal-derived oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, Examples of the fatty acids include petrolatum, paraffin wax, squalane, etc.; myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid, etc.; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, stearyl alcohol, etc.; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate), etc.

[0023] Examples of the surfactant include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkyl phenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of the surfactants that can be used include anionic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0024] Examples of emulsifiers and emulsifier aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or its derivatives, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or its esters, lactose, soy-derived water-soluble polysaccharides, soy-derived protein and polysaccharide complexes, lanolin or its derivatives, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and its derivatives, lecithin and its derivatives (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0025] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acids octyldodecyl, seaweed extracts, estradiol, various amino acids and their derivatives.

[0026] Examples of thickening agents include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin, pullulan, and aloe polysaccharides; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0027] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0028] Examples of preservatives and disinfectants include urea; benzoic acid or its salts, paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and alkane bromide. These include kilisoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, fermented radish liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0029] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0030] Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0031] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybeans, adzuki beans, etc.), etc.

[0032] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0033] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (for example, tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.).

[0034] Examples of sequestering agents include disodium edetate, trisodium edetate, tetrasodium edetate, etidronic acid or a salt thereof (e.g., tetrasodium etidronic acid), gluconic acid or a salt thereof (e.g., sodium gluconate), diethylenetriaminepentaacetic acid or a salt thereof (e.g., pentasodium diethylenetriaminepentaacetic acid), hydroxyethanediphosphonic acid or a salt thereof (e.g., tetrasodium hydroxyethanediphosphonic acid), and phytic acid or a salt thereof (e.g., sodium phytate).

[0035] Examples of pigmentation prevention agents include one or more selected from kojic acid or its derivatives, ascorbic acid or its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, vitamin E or its derivatives, nicotinic acid or its derivatives, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), placenta extract, and linoleic acid.

[0036] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and L-ascorbic acid 2-sulfate. Ascorbic acid ester salts such as magnesium, ascorbic acid sugar derivatives such as L-ascorbic acid-2-glucoside (2-O-α-D-glucopyranosyl-L-ascorbic acid), L-ascorbic acid-5-glucoside (5-O-α-D-glucopyranosyl-L-ascorbic acid), 6-acylated derivatives of these ascorbic acid sugar derivatives (the acyl group is a hexanoyl group, an octanoyl group, a decanoyl group, etc.), L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc. Examples of the hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside) and α-arbutin (hydroquinone-α-D-glucopyranoside). Examples of the resorcinol derivatives include, for example, 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, 2-hydroxy-5-propionyloxybenzoic acid, etc.; examples of nicotinic acid derivatives include nicotinamide and benzyl nicotinate; examples of vitamin E derivatives include vitamin E nicotinate and vitamin E linoleate; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0037] Anti-wrinkle agents include vitamin A or its derivatives, vitamin E or its derivatives (tocopherol acetate, etc.), vitamin C or its derivatives (ascorbic acid glucoside, 3-O-ethyl ascorbic acid, magnesium ascorbyl phosphate, etc.), pantothenyl alcohol, tranexamic acid, and niacinamide.

[0038] Furthermore, it is also possible to use in combination components derived from natural products such as the following plants or microorganisms. For example, collagen or a hydrolysate thereof, yeast extract or hydrolysate, lactic acid bacteria culture, Gramineae plants, Cruciferae plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraeae plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, and Anacardiaceae plants. Examples of such extracts include extracts of one or more plants selected from the family Gardenia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Laminaceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0039] As the components derived from plants of the Gramineae family, in particular, hydrolyzed rice leaves, hydrolyzed rice extracts, hydrolyzed rice bran extracts, hydrolyzed germinated brown rice, fermented rice liquid, sake lees extracts derived from sake, and bamboo shoot skin extracts of Madake or Moso bamboo are preferred. As the components derived from plants of the Brassicaceae family, white mustard extracts or hydrolyzates thereof, and fermented white mustard are preferred. As the components derived from plants of the Theaceae family, in particular, green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, honey-scented black tea, etc.) are preferred. As the components derived from plants of the Rosaceae family, extracts of Damask rose flowers, extracts of peach flowers, leaves or immature fruits, extracts of strawberry flowers, extracts of cherry blossoms or leaves, and extracts of apricot fruits or seeds are preferred. As the component derived from a plant of the family Paeoniaceae, an extract of the root or flower of the peony and an extract of the flower or root of the peony are preferred. As the component derived from a plant of the family Amaranthaceae, an extract of Salicornia herbacea is particularly preferred. As the component derived from a plant of the family Zosteraea, an extract of Zostera marina or Zostera koa is particularly preferred. As the component derived from a plant of the family Fabaceae, an extract of white soybean or black soybean or a hydrolyzate thereof, or a fermented soy milk liquid, an extract of adzuki bean, an extract of red clover, and an extract of pueraria lobata root are particularly preferred. As the component derived from a plant of the family Asteraceae, an extract of burdock root, an extract of sunflower sprouts, an extract of Acanthus gracilis, an extract of Arnica officinalis, or an extract of chamomile flower are particularly preferred. As the component derived from a plant of the family Malvaceae, a fermented product of hibiscus, Rose of Sharon, or Hibiscus is preferred. As the component derived from a plant of the family Gentianaceae, an extract of gentiana is preferred. As the plant of the family Lamiaceae, an extract of perilla frutescens and an extract of the fruit of Acanthus purpurea are preferred. As the component derived from a plant of the Nelumbinaceae family, particularly preferred is a lotus flower or lotus seed extract or a fermented lotus seed product. As the component derived from a plant of the Cucurbitaceae family, particularly preferred is a loofah extract. As the component derived from a plant of the Araliaceae family, a ginseng extract or fermented product is preferred. As the component derived from a plant of the Solanaceae family, an extract of eggplant (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.) is included. As the component derived from a plant of the Bignoniaceae family, a Pau d'Arco bark extract is preferred. As the component derived from a plant of the Actinidiaceae family, an unripe kiwi extract is preferred.As the component derived from a plant of the family Mulberry, extract of mulberry fruit, and extract of fruit or bark of fig are preferable. As the component derived from a plant of the family Rhamnaceae, extract of jujube fruit is preferable. As the component derived from a plant of the family Iridaceae, saffron is preferable. As the component derived from a plant of the family Campanulaceae, extract or hydrolysate of root of Codonopsis arvensis is preferable. As the component derived from a plant of the family Anacardiaceae, mango fruit extract is particularly preferable. As the component derived from a plant of the family Garcinia, mangosteen fruit extract is particularly preferable. As the component derived from a plant of the family Valenciaceae, cherimoya fruit extract is preferable. As the component derived from a plant of the family Rutaceae, extract of Satsuma mandarin, bergamot fruit, extract of grapefruit or Banpeiyu fruit (including immature fruit), extract containing flavonoids and their glycosides contained in plants such as grapefruit or Hassaku, or extract of Japanese pepper seed are preferable. As the component derived from a plant of the Liliaceae family, an extract of Daylily, Daylily, Casablanca, Madonna lily, or Lilium longiflorum is preferred. As the component derived from a plant of the Crassulaceae family, an extract or fermented product of Rhodiola rosea is particularly preferred. As the component derived from a plant of the Oleaceae family, an extract of Jasmine flower is particularly preferred. As the plant of the Cupressaceae family, an extract of Juniperus communis fruit is particularly preferred. As the component derived from a plant of the Myrtaceae family, an extract of guava leaf is particularly preferred. As the plant of the Orchidaceae family, an extract of Bletilla serrata root (white orchid) is particularly preferred. As the component derived from a plant of the Convolvulaceae family, an extract of sweet potato or a fermented product thereof, or an extract of sweet potato shochu lees or a fermented product thereof is preferred. As the component derived from a plant of the Asparagaceae family, an extract of asparagus is particularly preferred. As the component derived from Laminaceae seaweed, particularly preferred is Laminaria extract, as the component derived from Laminaceae seaweed, particularly preferred is Acanthus extract, as the component derived from Ulvaeaceae seaweed, particularly preferred is Ulva pertusa extract, as the component derived from Funoriaceae seaweed, particularly preferred is Funori extract.

[0040] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples and Test Examples, but the present invention is not limited thereto. In the following, all parts are by weight and all % are by weight.

[0041] Production Example 1. Preparation of Rutaceae Plant Extract (1) The peel of Tachibana (Citrus tachibana) of the Rutaceae family was dried, and 500 g of purified water and 500 g of 1,3-butylene glycol were added to 100 g of the dried product, followed by stirring and extraction for 3 hours at 60° C. After the extraction operation, the crude extract was filtered to obtain 1,350 g of a light yellow-brown Tachibana peel extract (solid content concentration 1.87%).

[0042] Production Example 2. Preparation of Rutaceae Plant Extract (2) In Production Example 1, 1,338 g of a light yellow-brown Tachibana peel extract was obtained by the same procedure as in Production Example 10, except that 1,000 g of purified water was used instead of 500 g of purified water and 500 g of 1,3-butylene glycol as the extraction solvent (solid content concentration: 1.80%).

[0043] Preparation Example 3. Preparation of Rutaceae Plant Extract (3) In Production Example 1, 1,360 g of a light yellow-brown Tachibana peel extract was obtained by the same procedure as in Production Example 10, except that 500 g of purified water and 500 g of ethanol were used as the extraction solvent (solid content concentration: 1.90%).

[0044] Preparation Example 4. Preparation of Rutaceae plant extract (4) The peel of Cala mandarin, which belongs to the genus Citrus in the Rutaceae family, was dried, and 500 g of 1,3-butylene glycol and 500 g of purified water were added to 100 g of the dried product, followed by stirring and extraction for 3 hours at 60° C. After the extraction operation, the crude extract was filtered to obtain 820 g of a light yellow-brown Cala mandarin peel extract (solid content concentration 2.05%).

[0045] Preparation Example 5. Preparation of Rutaceae Plant Extract (5) In Production Example 4, 805 g of pale yellow-brown calamandarin peel extract was obtained by the same procedure as in Production Example 4, except that 1,000 g of purified water was used instead of 500 g of purified water and 500 g of 1,3-butylene glycol as the extraction solvent (solid content concentration: 2.00%).

[0046] Preparation Example 6. Preparation of Rutaceae Plant Extract (6) In Production Example 4, 830 g of pale yellow-brown calamandarin peel extract was obtained (solid content concentration 2.10%) by the same procedure as in Production Example 4, except that 500 g of purified water and 500 g of ethanol were used as the extraction solvent instead of 500 g of purified water and 500 g of 1,3-butylene glycol.

[0047] Test Example 1. Evaluation of the effect of promoting the expression of filaggrin Normal human epidermal cells (NHEK) were cultured in a 24-well plate at 3 × 10 4The cells were seeded at 1.000 cells / well and cultured at 37°C under 5% CO2 and saturated steam. Test sections were set up to add each of the sample solutions from Preparation Examples 1 and 4, and after 24 hours of culture, each sample solution was added, and normal human epidermal cells NHEK were further cultured for 48 hours. Here, the concentration of each sample solution was added to 1.0% as a solution. As a comparison control, a control section was set up in which a culture solution containing only 50% BG solution of the same concentration was added instead of the above sample solution, and cultured in the same manner. Next, the cells in each test section and control section were collected with 0.5 mL of ISOGEN II reagent (manufactured by Nippon Gene Co., Ltd.). 200 μL of RNase-free water was added to the collected cells, which were stirred and mixed, left at room temperature for 15 minutes, and then centrifuged for 15 minutes at 15,000 rpm and 4°C using a centrifuge (manufactured by TOMY Co., Ltd. / MX-160), and 500 μL of the supernatant was taken out. 500μL of isopropanol (Wako Pure Chemical Industries, Ltd.) was added to the collected supernatant, stirred and mixed, left at room temperature for 10 minutes, and then centrifuged at 15,000 rpm and 4℃ for 10 minutes to obtain a precipitate of total RNA. 0.5mL of 75% ethanol was added to the total RNA, and the precipitate was collected by centrifugation at 15,000 rpm and 4℃ for 3 minutes. The supernatant was discarded, and this process was repeated twice. After completely removing the supernatant, the mixture was air-dried and dissolved in RNase-free water. The collected total RNA was reverse-transcribed using a designated kit (PrimeScript RT reagent Kit with gDNA Eraser (Perfect Real Time) [Takara Bio]) to synthesize cDNA. Using the synthesized cDNA as a sample, the expression of various genes and the expression of the internal standard GAPDH gene were detected using Thermal Cycler Dice (registered trademark) Real Time System Single (manufactured by Takara Bio Inc.) and SYBR (registered trademark) Premix Ex TaqTM (Perfect Real Time) [manufactured by Takara Bio Inc.].Here, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is one of the housekeeping genes (a gene that is expressed at a constant level in many tissues and cells, is constantly expressed, and is essential for the maintenance and proliferation of cells), and since its expression level is always constant, it is used as an internal standard in PCR experiments. The test results were obtained by comparing the expression levels of each gene in each test group when the expression level of the GAPDH gene was kept constant. The expression level in the control group was set to 100% and a relative value was calculated.

[0048] The results of Test Example 1 are shown in Table 1. [Table 1] JPEG2025004740000001.jpg42126

[0049] As shown in Table 1, Test Example 1 confirmed that the extracts according to the present invention (extracts of Production Examples 1 and 4) significantly promote the expression of filaggrin. In the stratum corneum, filaggrin is deiminated by an enzyme to become deiminated filaggrin, and this deiminated filaggrin is decomposed by an enzyme to produce amino acids (NMFs) that are natural moisturizing factors. Therefore, applying the extracts of Production Examples 1 and 4 to the skin promotes the skin cells to produce natural moisturizing factors themselves, providing a sustained feeling of moisture and also enabling sustained improvement in transparency and luster.

[0050] Test Example 2: Evaluation test of the effect of promoting tight junction synthesis Normal epidermal cells (NHEK) were cultured in a 96-well plate at 1.5 x 10 4The cells were seeded at 1000 cells / well and cultured at 37°C under 5% CO2 and saturated water vapor. The cells were cultured for 48 hours in the well to make them subconfluent. Next, the extract according to the present invention (extracts of Production Examples 1 and 4) was added as an evaluation sample, and the cells were cultured for another 48 hours. The sample was adjusted to 1.0% as a solution. In addition, as a comparative control, a control group was set in which a culture solution containing only a 30% BG solution (1.0%) of the same concentration was added instead of the sample. After completion, the culture solution was removed, the cells were washed with D-PBS (containing no Ca or Mg, hereinafter referred to as PBS), and PBS containing 10% trichloroacetic acid was added as a fixative, and the cells were allowed to stand at 4°C for 30 minutes. After removing the fixative and washing with PBS, PBS containing 0.2% Triton-X (hereinafter referred to as wash solution) was added and incubated at room temperature for 1 hour. Then, the cells were incubated overnight at 4°C using Blocking One P (manufactured by Nacalai Tesque) as a blocking agent. After washing once with washing solution, anti-occludin (Life Span Bio Sciences) and anti-claudin-1 antibody (Bioward Technology) were added as primary antibodies at 500-fold dilution, and further incubated overnight at 4°C. After washing three times with washing solution, Alexa Fluor (registered trademark) 488 Goat Anti-mouse IgG and Alexa Fluor (registered trademark) 546 Goat Anti-rabbit IgG (both manufactured by Invitrogen) were added as secondary antibodies at 500-fold dilution. After incubation at room temperature for 2 hours, washing was performed five times with washing solution. Then, Hoechst 33342 (Dojindo Laboratories) was added at 1000-fold dilution with PBS, and incubated for 30 minutes. The fluorescence intensity of each antibody and Hoechst 33342 was measured using a Fluoroskan Ascent (ThermoLabsystems). The fluorescence intensity of the antibody was corrected using the Hoechst 33342 value as an internal standard, and then the detection value of each antibody was calculated as a relative value with the control group set as 100%.

[0051] The results of Test Example 2 are shown in Table 2. [Table 2] JPEG2025004740000002.jpg70127

[0052] As shown in Table 2, Test Example 2 confirmed that the extract according to the present invention (extracts of Production Examples 1 and 4) exhibits the effect of promoting the synthesis of proteins (occludin, claudin-1) that constitute tight junctions, which are binding structures between cells. This suggests that the extract according to the present invention can improve the barrier function of the skin and prevent evaporation of moisture from the skin, thereby providing the skin with a sustained moist feeling. It also suggests that the extract exhibits the effect of preventing the invasion of bacteria and foreign substances (environmental pollutants, etc.) into the skin from the outside.

[0053] Test Example 3. Evaluation test of increased activity of oxidized ascorbate reductase NHEK epidermal cells were cultured in HuMedia KG2 medium (Kurabo) at 8.0 × 10 4The cells were seeded per well, and cultured for 24 hours under conditions of 37°C and 5% CO2, then replaced with HuMedia KB2 medium (Kurabo) containing each sample solution, and further cultured under the same conditions for 48 hours. Here, the extract of Production Example 4 was adjusted to a final concentration of 1.0% in the medium. After the culture was completed, the cells were detached using trypsin and collected in a microtube using PBS(-) containing 5% FBS. Then, the cells were washed twice with PBS(-), the supernatant was removed, and the cells were rapidly frozen using liquid nitrogen and stored at -80°C until the enzyme activity was measured. In this Test Example 4, the activity of an enzyme that reduces oxidized ascorbic acid (dehydroascorbic acid) (hereinafter referred to as "DHA reductase") was measured. The DHA reductase activity was measured as follows. 500 μL of Passive Lysis Buffer (Promaga) was added to the stored cells, and the cells were dissolved by pipetting. After centrifugation (12000g x 5 min, 4°C), the supernatant was collected and used as a crude enzyme solution. 50 μL of the obtained crude enzyme solution was added per well, and 50 μL of purified water (control) or 50 μL of 2 mM dehydroascorbic acid solution (test) was added. 50 μL of DTNB (5,5'-dithio-bis- (2-nitrobenzoic acid)) test solution (CAYMAN CHEMICAL COMPANY) was added, and the absorbance at 414 nm was measured. The difference obtained by subtracting the value of the test group from the value of the control group for each sample addition group was used as the DHA reductase activity. Meanwhile, the protein concentration of the crude enzyme solution was measured by the Bradford method using Protein Assay Dye (BIO-RAD), and the value obtained by dividing the absorbance difference by the protein concentration was used as the DHA reductase activity per protein for each sample addition group, and the relative value was calculated by taking the value of the solvent control addition group as 100.

[0054] The results of Test Example 3 are shown in Table 3. [Table 3] JPEG2025004740000003.jpg41127

[0055] As shown in Table 3, Test Example 3 confirmed that the extract according to the present invention (extract of Production Example 4) activates DHA reductase. As a result, the extract according to the present invention can reduce oxidized ascorbic acid (dehydroascorbic acid) that has been oxidized in the body and lost its functions such as antioxidant effect, returning it to the original ascorbic acid, thereby promoting the exertion of its functions.

[0056] Formulation example 1. Lotion [Ingredients] Part Extract of Preparation Example 1 2.0 Squalane 0.2 Mandarin Orange Oil 0.01 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Isopropyl methylphenol 0.1 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Purified water (enough to make the total amount 100 parts)

[0057] Formulation example 2: Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that, among the ingredients of Formulation Example 1, 2.0 parts of the extract of Production Example 2 was used instead of 2.0 parts of the extract of Production Example 1.

[0058] Formulation example 3. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that, among the ingredients of Formulation Example 1, 2.0 parts of the extract of Production Example 3 was used instead of 2.0 parts of the extract of Production Example 1.

[0059] Formulation example 4: Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that, among the ingredients of Formulation Example 1, 2.0 parts of the extract of Production Example 4 was used instead of 2.0 parts of the extract of Production Example 1.

[0060] Formulation example 5. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that, among the ingredients of Formulation Example 1, 2.0 parts of the extract of Production Example 5 was used instead of 2.0 parts of the extract of Production Example 1.

[0061] Formulation example 6. Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that, among the ingredients of Formulation Example 1, 2.0 parts of the extract of Production Example 6 was used instead of 2.0 parts of the extract of Production Example 1.

[0062] Formulation example 7. Lotion [Ingredients] Part Extract of Preparation Example 1 0.5 Glyceryl Caprylate 3.0 Polyglyceryl-10 Laurate 3.0 Mandarin Orange Oil 0.1 Cetanol 2.0 Behenyl Alcohol 2.0 Niacinamide 5.0 Dipotassium glycyrrhizinate 0.1 Glycerin 1.0 1,3-Butylene glycol 1.0 Pentanediol 1.0 Potassium hydroxide 0.5 Purified water (enough to make the total amount 100 parts)

[0063] Formulation example 8. Lotion A lotion was obtained in the same manner as in Formulation Example 7, except that 0.5 parts of the extract from Preparation Example 4 was used instead of 0.5 parts of the extract from Preparation Example 1.

[0064] Formulation example 9. Lotion [Ingredients] Part Extract of Preparation Example 1 0.25 Avocado oil 0.1 Olive fruit oil 0.1 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Methylparaben 0.1 Ascorbic Acid Glucoside 2.0 Niacinamide 5.0 ε-Aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Pyridoxine hydrochloride 0.5 Glycerin 5.0 1,3-Butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water (enough to make the total amount 100 parts)

[0065] Formulation example 10. Lotion A lotion was obtained in the same manner as in Formulation Example 9, except that 0.25 parts of the extract from Preparation Example 4 was used in place of 0.25 parts of the extract from Preparation Example 1.

[0066] Formulation example 11. Emulsion [Ingredients] Part Extract of Preparation Example 1 1.0 Squalane 5.0 Cyclopentanesiloxane 1.0 Hexalan 3.0 Hexyldecyl Isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 Laurate 5.0 Polyglyceryl-10 Isostearate 5.0 Ascorbyl Dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Ascorbic acid phosphate magnesium salt 3.0 Mandarin Orange Oil 0.01 Arbutin 3.0 Glycerin 3.0 1,3-Butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropyl methylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed Collagen 1.0 Acetyl hyaluronic acid 0.1 Disodium edetate 0.05 Diethylenetriaminepentaacetic acid pentasodium 0.05 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0067] Formulation example 12. Lotion A lotion was obtained in the same manner as in Formulation Example 11, except that, among the ingredients of Formulation Example 11, 1.0 part of the extract of Preparation Example 4 was used instead of 1.0 part of the extract of Preparation Example 1.

[0068] Formulation example 13. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 2.0 parts of L-ascorbic acid-2-glucoside was used in place of 2.0 parts of magnesium ascorbyl phosphate in Formulation Example 11.

[0069] Formulation example 14. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 2.0 parts of tranexamic acid were used in place of 2.0 parts of magnesium ascorbyl phosphate.

[0070] Formulation example 15. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 3.0 parts of 3-O-ethyl ascorbic acid was used in place of 2.0 parts of magnesium ascorbyl phosphate.

[0071] Formulation example 16. Emulsion An emulsion was obtained in the same manner as in Formulation Example 11, except that 2.0 parts of ascorbic acid phosphate magnesium salt and 5.0 parts of niacinamide were used in place of potassium hydroxide.

[0072] Prescription example 17. Cream [Ingredients] Part Extract of Preparation Example 1 1.0 Olive oil 5.0 Mandarin Orange Oil 1.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.0 Candelilla Wax 1.0 Lactic acid fermented rice 2.0 Soybean-derived hydrogenated lecithin 0.5 Carboxyvinyl Polymer 1.0 Sodium alginate 1.0 Glycerin 2.0 Pentanediol 1.0 PH adjuster (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0073] Prescription example 18. Cream A cream was obtained in the same manner as in Formulation Example 17, except that 1.0 part of the extract from Preparation Example 4 was used instead of 1.0 part of the extract from Preparation Example 1 among the ingredients of Formulation Example 17.

[0074] Prescription example 19. Cream [Ingredients] Part Extract of Preparation Example 1 0.25 Olive oil 5.0 Jojoba oil 5.0 Mandarin Orange Oil 1.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Di(octyldodecyl) lauroyl glutamate / phytosteryl / Behenyl) 5.0 Glyceryl Caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-Oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic acid 2.5 D-Pantothenyl Alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Niacinamide 5.0 Lactic acid fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Hydrolyzed Collagen 1.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-Camphor 0.3 l-Menthol 0.5 Purified water (enough to make the total amount 100 parts)

[0075] Prescription example 20. Cream A cream was obtained in the same manner as in Formulation Example 19, except that 0.25 parts of the extract from Preparation Example 4 was used instead of 0.25 parts of the extract from Preparation Example 1.

[0076] Example 21. Pack [Ingredients] Part Extract of Preparation Example 1 1.0 Dipropylene glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetanol 3.0 Behenyl Alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Water-soluble collagen 1.0 Xanthan gum 2.0 Polyglyceryl-6 Myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated Lecithin 3.0 Hydroxylated Lecithin 3.0 Purified water (enough to make the total amount 100 parts)

[0077] Prescription example 22. Cream A cream was obtained in the same manner as in Formulation Example 21, except that 1.0 part of the extract from Preparation Example 4 was used instead of 1.0 part of the extract from Preparation Example 1 among the ingredients of Formulation Example 21.

[0078] Prescription example 23. Hair shampoo [Ingredients] Part Extract of Preparation Example 1 2.0 Sodium Laureth Sulfate 10.0 Glyceryl Monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl Alcohol 2.0 Behenyl Alcohol 2.0 Dimethicone 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium Salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene glycol 2.0 Purified water (enough to make the total amount 100 parts)

[0079] Prescription example 24. Shampoo A shampoo was obtained in the same manner as in Formulation Example 23, except that, among the ingredients of Formulation Example 23, 1.0 part of the extract of Preparation Example 4 was used instead of 1.0 part of the extract of Preparation Example 1.

[0080] Formulation example 25. Hair conditioner [Ingredients] Part Extract of Preparation Example 1 1.0 Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetanol 3.0 Stearyl alcohol 1.0 Allantoin 0.1 Isopropyl methylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water (enough to make the total amount 100 parts)

[0081] Formulation example 26. Hair conditioner A hair conditioner was obtained in the same manner as in Formulation Example 25, except that 1.0 part of the extract from Production Example 4 was used instead of 1.0 part of the extract from Production Example 1.

[0082] Formulation example 27. Cleansing cosmetics [Ingredients] Part Extract of Preparation Example 1 2.0 Potassium cocoyl glycine 5.0 Glycerin 10.0 Glyceryl Caprylate 1.0 Sodium lauroyl aspartate 10.0 Water-soluble collagen 5.0 Cetanol 3.0 Myristyl Alcohol 3.0 Isopropyl methyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.05 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.2 Triclosan 0.1 Trichlorocarbanide 0.5 Trichlorohydroxydiphenyl ether 0.2 Concentrated benzalkonium chloride solution 50 0.2 Benzalkonium chloride 0.1 Purified water (enough to make the total amount 100 parts)

[0083] Formulation example 28. Cleansing cosmetics A cleansing cosmetic was obtained in the same manner as in Formulation Example 28, except that, among the ingredients of Formulation Example 27, 1.0 part of the extract of Production Example 4 was used instead of 1.0 part of the extract of Production Example 1.

[0084] Formulation example 29. Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following components. [Ingredients] Part Extract of Preparation Example 1 1.0 Niacinamide 1.0 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 0.1 Water-soluble collagen 0.1 Sodium hyaluronate 0.01 Glycerin 1.0 1,3-Butylene glycol 2.0 Pentanediol 1.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0085] Formulation example 30. Sheet mask A sheet mask was obtained in the same manner as in Formulation Example 29, except that 1.0 part of the extract from Production Example 4 was used instead of 1.0 part of the extract from Production Example 1 among the ingredients of Formulation Example 29.

[0086] Prescription example 31. Beauty serum [Ingredients] Part Extract of Preparation Example 1 1.0 Sodium hyaluronate 1.0 Water-soluble collagen 1.0 Tranexamic acid 0.1 Ethanol 2.0 Glycerin 5.0 1,3-Butylene glycol 5.0 Methylparaben 0.1 Citric acid 0.3 Sodium citrate 0.6 Eucheuma muscaria extract 5.0 Purified water (enough to make the total amount 100 parts)

[0087] Prescription example 32. Beauty serum A beauty essence was obtained in the same manner as in Formulation Example 31, except that, among the ingredients of Formulation Example 31, 1.0 part of the extract of Production Example 4 was used instead of 1.0 part of the extract of Production Example 1.

[0088] Prescription example 33. Cream [Ingredients] Part Extract of Preparation Example 1 0.25 Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Shea Butter 2.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.5 Candelilla Wax 0.5 Niacinamide 1.0 Lactic acid fermented rice 3.0 Hydrogenated Lecithin 2.0 Eucheuma muscaria extract 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Glycerin 4.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total amount 100 parts)

[0089] Prescription example 34. Cream A cream was obtained in the same manner as in Formulation Example 33, except that 1.0 part of the extract from Preparation Example 4 was used instead of 1.0 part of the extract from Preparation Example 1.

Claims

1. An external skin preparation that continuously improves the skin's moisture, transparency and luster, containing as active ingredients either one or two extracts of Tachibana (Citrus tachibana) and Calamandarin (Citrus unshiu x Citrus noblis).

2. An activator of oxidized ascorbate reductase, the active ingredient of which is an extract of Calamandarin (Citrus unshiu × Citrus noblis).

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