Lettuce plant

Abstract

To provide a novel lettuce variety.SOLUTION: The present disclosure provides a seed, a plant, and a tissue culture of a lettuce plant identified by Accession No. FERM BP-22478 and a method for producing a lettuce plant produced by crossing the lettuce plant identified by Accession No. FERM BP-22478 with any other lettuce plant.SELECTED DRAWING: None

Description

[Technical field]

[0001] The present invention relates to lettuce plants. [Background technology]

[0002] Lettuce plants prefer cool climates. In recent years, they have suffered from diseases such as root rot and poor growth, which are thought to be caused by continuous cultivation and abnormal weather conditions such as high temperatures and humidity, and producers are looking for varieties that can be produced stably. [Prior art documents] [Non-patent literature]

[0003] [Non-Patent Document 1] M. FUJINAGA, “Studies on physiological races and phylogenetic analysis of lettuce root rot pathogen, Fusarium oxysporum f. sp. Lactucae”, Journal of General Plant Pathology volume 71, page457 (2005) [Non-Patent Document 2] T. SHIMIZU et.al., “Development Trend of Diseases on Lettuce (Lactuca sativa) of Organic Cultivation in Highlands of Nagano Prefecture and Their Control”, Annual report of the Kanto-Tosan Plant Protection Society 64:2017.12 p.41-46 Summary of the Invention [Problem to be solved by the invention]

[0004] Therefore, an object of the present invention is to provide a new lettuce plant. [Means for solving the problem]

[0005] To achieve the above objectives, representative seeds of the lettuce plant of the present invention have been deposited under the accession number FERM BP-22478.

[0006] Lettuce plants of the present invention are grown from seeds identified under Accession No. FERM BP-22478.

[0007] The method for producing a lettuce plant of the present invention includes a self-pollination step of self-pollinating the lettuce plant of the present invention.

[0008] The method for producing a lettuce plant of the present invention includes a crossbreeding step of crossbreeding the lettuce plant of the present invention with another lettuce plant. Effect of the Invention

[0009] According to the present invention, a new lettuce plant can be provided. [Brief description of the drawings]

[0010] [Figure 1] FIG. 1 is a schematic diagram showing an example of leaf top overlap and leaf lobe number of a lettuce plant. [Diagram 2] FIG. 2 is a schematic diagram showing examples of leaf shapes of lettuce plants. [Diagram 3] FIG. 3 is a schematic diagram showing examples of the depth of the marginal incision in the leaves of lettuce plants, the size of the bulb, and the shape of the longitudinal cross section of the bulb. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0011] <Lettuce plant> The lettuce plant of the present invention includes the lettuce plant (hereinafter also referred to as the "deposited strain") represented by TEXLE203026 and identified by the accession number FERM BP-22478 (deposited) or its progeny strain. The lettuce plant of the present invention is characterized by including the lettuce plant represented by TEXLE203026 and identified by the accession number FERM BP-22478 or its progeny strain, and may include new mutations, transgenes and / or single locus conversions, and other configurations and conditions are not particularly limited.

[0012] In the present invention, the "lettuce plant" is a plant classified in the genus Lactuca of the family Asteraceae, species L. sativa L., with the scientific name Lactuca sativa and the Japanese name "chisha" (lettuce, Lactuca, chisa). The lettuce plant may be, for example, a hybrid with a related species or a wild species.

[0013] In the present invention, the "cultivated lettuce plant", "cultivated lettuce variety", or "cultivated lettuce" is a lettuce plant or its variety, breeding line or cultivated variety that is cultivated by humans and is excellent in cultivation science. The "cultivated lettuce plant", "cultivated lettuce variety", or "cultivated lettuce" may be their hybrids or hybrids with other lettuce species.

[0014] In the present invention, the "plant" means an individual plant representing the whole plant.

[0015] In the present invention, the term "plant part" refers to a part of an individual plant. Examples of the "plant part" include plant cells, plant protoplasts, plant cell cultures or tissue cultures capable of regenerating plants, plant callus, plant clumps, plant cells isolated from plants or plant parts, leaves, heads, expanded leaves, pollen, embryos, cotyledons, hypocotyls, roots, root tips (root tips), anthers, pistils, flowers, ovaries, ovules, seeds, fruits, stems, seedlings, etc. Examples of the part of the individual plant include organs, tissues, cells, vegetative propagules, etc., and any of these may be used. Examples of the organs include petals, corollas, flowers, leaves, seeds, fruits, stems, roots, etc. Examples of the tissues are parts of the organs. Specific examples of the part of the plant individual include microspores, flowers, flower buds, pistils, anthers, pollen, ovaries, embryos, ovules, hypocotyls, embryo sacs, egg cells, cuttings, roots, root tips, stems, stalks, leaves, heads, expanded leaves, petioles, pith, cotyledons, cells, meristematic cells, protoplasts, seeds, and the like. The pollen may be mature or immature pollen. The part of the plant individual may be derived from any growth stage of a plant, for example, before rooting, after rooting, seedlings, cuttings, mature individuals, and the like. The part of the plant individual may be, for example, one type of organ, tissue, and / or cell, or two or more types of organs, tissues, and / or cells.

[0016] In the present invention, "root rot (lettuce root rot)" is a disease caused by a filamentous fungus. Examples of the pathogenic fungus causing the root rot include Fusarium oxysporum f. sp. Lactucae. The "race" refers to a fungus lineage with different pathogenicity, more specifically, a lineage that shows different pathogenicity against varieties with different resistance genes or resistance loci.

[0017] In the present invention, "resistance" is also referred to as, for example, "disease resistance." The resistance means, for example, the ability to inhibit or suppress the occurrence and progression of a disease caused by infection with a pathogen, and specifically may mean, for example, the absence of disease, the halting of the progression of an already occurring disease, and the suppression (also referred to as "inhibition") of the progression of an already occurring disease.

[0018] In the present invention, the "root rot resistance" can be evaluated according to the measurement method of trait number 59 described below. In the present invention, the lettuce plant exhibiting the "root rot resistance" may be resistant to one or more races, and may be resistant to multiple races. Preferably, the lettuce plant has resistance to root rot race 1.

[0019] <Deposited strain> The lettuce plant of the present invention is designated TEXLE203026, and a representative example thereof is the lettuce plant identified by the accession number FERM BP-22478 or its progeny line. Hereinafter, the accession number FERM BP-22478 is also referred to as the lettuce line TEXLE203026. Information regarding the deposit of the variety is shown below.

[0020] (TEXLE203026) Type of deposit: International deposit Name of depository institution: National Institute of Technology and Evaluation, Patent Biological Deposit Center Address: Room 120, 2-5-8 Kazusa Kamatari, Kisarazu City, Chiba Prefecture, Japan 292-0818 Accession number: FERM BP-22478 Identification mark: TEXLE203026 Received: May 18, 2023

[0021] The deposited line exhibits, for example, the morphological and physiological characteristics described in Table 1 below. In Table 1 below, the morphological and physiological characteristics are based on cultivation in Yuma, Arizona, USA in 2022. In Table 1 below, the morphological and physiological characteristics are evaluated based on the Lettuce Species Examination Criteria (issued in September 2022, https: / / www.maff.go.jp / j / shokusan / hinshu / info / kijun / 1361.pdf) issued by the Ministry of Agriculture, Forestry and Fisheries of Japan (MAFF). In addition, in Table 1 below, the morphological and physiological characteristics are evaluated based on the criteria described below. The morphological and physiological characteristics can be seen in Figures 1 to 3. In addition, in the evaluation of the morphological and physiological characteristics, the DUS test guidelines in UPOV may be referred to. As the DUS test guidelines, for example, the guidelines published on April 5, 2017 (https: / / www.upov.int / edocs / tgdocs / en / tg013.pdf) can be referred to.

[0022] [Table 1]

[0023] (Trait number 1) The "seed color" can be evaluated by visual observation. The "seed color" can be evaluated based on the following criteria: Class 1 (white, standard variety: Olympia), Class 2 (yellow), Class 3 (brown, standard variety: Cisco, V lettuce), and Class 4 (black, standard variety: Logic).

[0024] (Trait number 4) The "plant width" refers to the maximum diameter (cm) of the plant, and can be evaluated by measurement. The "plant width" can be evaluated based on the following criteria: Class 1 (very small), Class 3 (small, standard variety: Okayama salad greens), Class 5 (medium, standard variety: Olympia), Class 7 (large), and Class 9 (very large).

[0025] (Trait number 5) The "overlap of the upper leaves of the plant" refers to the degree of overlap of the upper leaves of the plant (degree of head formation) and can be evaluated by visual observation. The "overlap of the upper leaves of the plant" can be evaluated based on the following criteria: Class 1 (none or weak, standard variety: Prizehead), Class 2 (medium, standard variety: Costa Rica No. 4), and Class 3 (strong, standard varieties: Olympia and Vanguard 75).

[0026] (Trait number 8) The "number of leaf lobes" refers to the number of leaf lobes, and can be evaluated by visual observation. The "number of leaf lobes" can be evaluated based on the following criteria: class 1 (none or very small, standard variety: Olympia), class 3 (few), class 5 (medium), class 7 (many), and class 9 (very many).

[0027] (Trait number 9) The "leaf shape" refers to the shape of the leaves of varieties with no or very few leaf lobes (limited to varieties with no or very few leaf lobes), and can be evaluated by visual observation. The "leaf shape" can be evaluated based on the following criteria: class 1 (triangular), class 2 (lanceolate), class 3 (oblate, standard variety: Olympia), class 4 (narrow oblate), class 5 (circular, standard variety: White Boston), class 6 (broad elliptical), class 7 (elliptical, standard variety: Costa Rica No. 4), class 8 (narrow elliptical, standard variety: Red Kakisha), class 9 (linear), class 10 (broad obovate), class 11 (obovate, standard variety: Prizehead), and class 12 (oblanceolate).

[0028] (Trait number 13) The "anthocyanin coloration of the leaves" refers to the intensity of the anthocyanin coloration of the leaves, and can be evaluated by visual observation. The "anthocyanin coloration of the leaves" can be evaluated based on the following criteria: Class 1 (none or very weak), Class 3 (weak), Class 5 (medium, standard variety: Prizehead), Class 7 (strong), and Class 9 (very strong).

[0029] (Trait number 22) The "leaf unevenness" refers to the strength of the unevenness (shrinkage) of the leaf surface, and can be evaluated by visual observation. The "leaf unevenness" can be evaluated based on the following criteria: Class 1 (none or very weak, standard variety: Cisco), Class 3 (weak, standard variety: Olympia), Class 5 (medium, standard variety: Early Impulse), Class 7 (strong, standard variety: Nishinabeni), and Class 9 (very strong, standard variety: Black Seeded Simson).

[0030] (Trait number 24) The "waviness of the leaf margin" refers to the strength of the waviness of the leaf margin, and can be evaluated by visual observation. The "waviness of the leaf margin" can be evaluated as grade 1 (none or very weak), grade 3 (weak), grade 5 (medium, standard variety: Mikado Great 3204), grade 7 (strong, standard variety: Calmar, Grand Rapid), and grade 9 (very strong).

[0031] (Trait number 26) The "depth of the incision at the edge of the leaf" refers to the depth of the incision at the edge of the leaf, and can be evaluated by visual observation. The "depth of the incision at the edge of the leaf" can be evaluated based on the following criteria: Class 1 (none or very shallow), Class 3 (shallow), Class 5 (medium, standard variety: Olympia), Class 7 (deep), and Class 9 (very deep).

[0032] (Trait number 30) The "bulb size" refers to the size of the bulb, and means the bulb size (bulb diameter x bulb height) of varieties with medium or strong overlap of the upper leaves of the plant (limited to varieties with medium or strong overlap of the upper leaves of the plant), and can be evaluated by measurement and visual observation. The "bulb size" can be evaluated based on the following criteria: class 1 (very small), class 3 (small), class 5 (medium, standard variety: Olympia), class 7 (large), and class 9 (very large).

[0033] (Trait number 31) The "shape of the bulb in longitudinal section" refers to the shape of the bulb in longitudinal section, and means the shape of the bulb in longitudinal section of a variety with medium or strong overlap of the upper leaves of the plant (limited to varieties with medium or strong overlap of the upper leaves of the plant), and can be evaluated by visual observation. The "shape of the bulb in longitudinal section" can be evaluated based on the following criteria: Class 1 (narrow oval), Class 2 (wide oval), Class 3 (circular, standard variety: Excel Headgrass), Class 4 (oblate, standard variety: Cisco).

[0034] (Trait number 40) The "initiation of bolting" refers to the time when bolting starts under long-day conditions (the time when 50% of the test plants have bolted is the number of days from the sowing date), and can be evaluated by measurement and visual observation. The "initiation of bolting" can be evaluated based on class 1 (very early, standard variety: Green Leaf), class 3 (early, standard variety: Prize Head), class 5 (medium, standard variety: Gett, Falcon), class 7 (late, standard variety: Olympia), and class 9 (very late).

[0035] (Trait number 59) The "resistance to race 1 of root rot fungi" means the degree of resistance to race 1 of root rot fungi. The "resistance to race 1 of root rot fungi" can be evaluated based on the criteria of class 1 (susceptibility, standard varieties: Patriot, late-flowering Red Fire, Waldman's Green), class 2 (moderate resistance, standard variety: Salinas 88), and class 3 (high resistance, standard variety: Costa Rica No. 4).

[0036] The resistance to race 1 of root rot fungus can be confirmed by the following test method. Testing Method Pathogen: Lettuce root rot fungus (Fusarium oxysporum f. sp. lactucae (Fol)) Race 1 is used. Race confirmation is performed using susceptible varieties. Pathogenicity confirmation: Pathogenicity is confirmed using susceptible varieties prior to testing. · Conducting the test Number of test specimens: At least 30 specimens, divided into two or more replicates (at least 20 specimens, divided into two or more replicates in the case of non-inoculated areas). Standard varieties: Susceptible; Patriot, late-flowering Red Fire, Waldman's Green; moderately resistant; Salinas 88; and highly resistant; Costa Rica No. 4. Temperature: Keep within the range of 20°C at night and 25-28°C during the day. Illumination: Natural light or a minimum of 15,000lx with a 14-hour light period. Inoculation preparation: After inoculating the test strain cultured in a nutrient medium (PSA medium, etc.) into the bran medium, mix and culture at approximately 25°C for 10 to 14 days. The medium with sufficient bacterial growth throughout the medium is used as the inoculum. Inoculation method: Add the inoculum to sterilized soil (or commercially available culture soil) at a volume ratio of 20:1 and mix well. Fill a pot or the like with this as contaminated soil, and sow healthy seeds after germination. If the seeds have poor germination, it will be difficult to determine whether they have died due to disease, so try not to sow seeds with poor germination. Test period: The severity of the disease is evaluated approximately 20 to 30 days after inoculation. Judgment criteria: Evaluate the degree of disease for each individual as follows, and calculate the disease index to make a judgment. Disease severity 0: No symptoms Severity of disease: 1: Mild stunting, growth suppression Severity 2: Severe stunting Disease severity 3: Death Disease index = Σ(disease level × number of individuals with each disease level) / (total number of individuals) Judgment: Evaluate by comparing with the disease index of each standard variety. In addition to the above test method, a seedling immersion test can also be performed. For details of the seedling immersion test method, see, for example, the UPOV test guideline (LETTUCE; TG / 13 / 11 Rev.2, https: / / www.upov.int / edocs / tgdocs / en / tg013.pdf).

[0037] In the present invention, a plant having "essentially all physiological and morphological characteristics of the deposited line" means a plant having the major traits of the deposited line from which the target plant is derived when grown in the same environment. The major traits are traits (1) to (13) below, i.e., trait numbers 30, 40, 59, 1, 4, 5, 8, 9, 13, 22, 24, 26, and 31 in Table 1. The major traits are preferably traits of trait numbers 30, 40, and 59 in Table 1, i.e., traits (1) to (3) below. The plant having "essentially all physiological and morphological characteristics of the deposited line" may be a plant having the same traits as the deposited line except for, for example, 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait, i.e., 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait may be a trait different from the deposited line. The "trait different from the deposited line" may be a major trait of the deposited line or a trait other than the major trait of the deposited line, but a trait other than the major trait of the deposited line is preferred. The "trait different from the deposited line" can be implemented, for example, by introducing a trait and / or a gene as described below. In a plant having "essentially all of the physiological and morphological characteristics of the deposited line," all of the traits of trait numbers 30, 40, 59, 1, 4, 5, 8, 9, 13, 22, 24, 26, and 31 may be the same as those of the deposited line. (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant”; (4) The seed color is "white"; (5) The width of the stock is "medium"; (6) The upper leaves of the plant have a strong overlap; (7) The number of leaf lobes is absent or very small; (8) The leaf shape is "narrow oblate"; (9) Anthocyanin coloration of leaves is absent or very weak; (10) The leaf texture is "medium"; (11) The leaf margins are somewhat wavier; (12) The depth of the incision on the edge of the leaf is "slightly deep"; (13) The cross-sectional shape of the leaf is "oblate."

[0038] <Progeny lineage> The lettuce plant of the present invention may be a progeny of the deposited line. The progeny may be an individual plant of the progeny, or a part of an individual plant of the progeny, or a seed of the progeny.

[0039] In the present invention, a "progeny line" or a "progeny lettuce plant" (hereinafter collectively referred to as "progeny line") is a lettuce plant of the deposited line or a plant obtained from the progeny line. In the present invention, the progeny line may be a plant obtained by crossing the deposited line with another lettuce plant, or crossing the deposited line with a wild lettuce plant. The progeny line may be obtained, obtainable, or derived directly or indirectly by selfing and / or cross-pollinating the deposited line or its progeny line, or may be derived from a parent line obtained from the deposited line using traditional breeding methods such as selfing and / or cross-pollination. Examples of the progeny line include a selfed progeny line, a first generation hybrid F1 (first generation hybrid line, F1 hybrid), and the like. In obtaining the progeny line, the deposited line may be used as a female parent, a male parent, or both parents.

[0040] In the present invention, "crossing" refers to the crossing of two parental lines. The crossing may be "cross-pollination" or "self-pollination". The cross-pollination refers to fertilization by the union of two gametes originating from different plants. The self-pollination refers to the transfer of pollen from anthers to the stigma of the same plant. The self-pollination may also be referred to as, for example, self-fertilization. The crossing may include backcrossing, which is one of the traditional breeding methods.

[0041] The "backcrossing" is one of the traditional breeding techniques, in which a breeder repeatedly backcrosses a progeny line of a hybrid with one of the parent lines to introduce a trait into a plant or variety. The plant containing the trait to be introduced can be called, for example, a donor plant. The plant into which the trait is introduced can be called, for example, a recurrent parent. The backcrossing can be performed by crossing a donor plant with a recurrent parent, and a first generation hybrid F1 (first generation hybrid line, F1 hybrid) can be obtained by the first crossing. The progeny line having the trait is then crossed with the recurrent parent. Then, the trait of the donor plant can be introduced into the recurrent parent by backcrossing and / or selfing for several generations.

[0042] In the present invention, the progeny line may be regenerated from a cell culture or tissue culture, a protoplast or a part of a plant individual derived from the deposited line, or may be obtained by self-pollination of the deposited line, or may be obtained by producing seeds from a plant individual of the deposited line.

[0043] In the present invention, the term "regeneration" refers to the generation or vegetative propagation of plants from cell culture, tissue culture or protoplasts.

[0044] The "tissue culture" or "cell culture" may be a composition containing isolated cells of the same or different types, or may be a collection of cells organized into a plant part. Methods for tissue culture of various tissues of lettuce plants and for regenerating plants from said tissue cultures are well known, see, for example, References 1 to 3 below. Reference 1: Pink DA and Carter PJ, “Propagation of lettuce (Lactuca sativa) breeding material by tissue culture”, Ann. Appl. Biol., 1987, vol. 110, pages 611-616 Reference 2: Ampomah-Dwamena C., Conner AJ and Fautrier AG, “Genotypic response of lettuce cotyledons to regeneration in vitro”, Sci. Hort., 1997, vol. 71, pages 137-145 Reference 3: Takayuki Mizutani, Takayuki Tanaka, "Nodal culture of flower stalks of lettuce and closely related wild species Lactuca serriola", Horticultural Research, 2008, Vol. 7, No. 1, pp. 17-21

[0045] The progeny line may have a desired trait. The progeny line may have, for example, "essentially all physiological and morphological characteristics of the deposited line" when cultivated under the same cultivation conditions. Specifically, the progeny line may have traits in common with the corresponding deposited line (derived deposited line). As a specific example, the progeny line may have, for example, one or more, two or more, three or more, four or more, five or more, six or more, seven or more, eight or more, nine or more, ten or more, eleven or more, twelve or more, or thirteen or more traits that match those of the deposited line. The progeny line may be a plant having the main traits of the deposited line. The main traits include traits of trait numbers 30, 40, and 59 in Table 1, i.e., the above traits (1) to (3). Moreover, the main traits include traits of trait numbers 1, 4, 5, 8, 9, 13, 22, 24, 26, and 31 in Table 1, i.e., the traits (4) to (13) above. The progeny line may be a plant having the same traits as the deposited line except for, for example, 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait, i.e., 13 or less, 12 or less, 11 or less, 10 or less, 9 or less, 8 or less, 7 or less, 6 or less, 5 or less, 4 or less, 3 or less, 2 or less, or 1 trait may be different from the deposited line. The "characteristics different from the deposited line" may be the main character of the deposited line or a character other than the main character of the deposited line, but a character other than the main character of the deposited line is preferable. The "characteristics different from the deposited line" can be implemented, for example, by introducing the character and / or gene described below. In the progeny line, all of the characters of the character numbers 30, 40, 59, 1, 4, 5, 8, 9, 13, 22, 24, 26, and 31 may be the same as those of the deposited line. Examples of the characteristics different from the deposited line include resistance to downy mildew (Bl16-37, Bl5-11US), resistance to race 2 of root rot fungus, resistance to bacterial rot disease, resistance to corky root disease, resistance to lettuce mosaic virus disease, resistance to aphids, resistance to leafminers, and resistance to browning of cut surfaces. Each trait can be introduced, for example, by crossing with a plant known to have a genetic locus associated with each trait.

[0046] The progeny line may include cells that contain at least one set of chromosomes derived from the deposited line. The progeny line may, for example, have at least 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% of its alleles derived from the deposited line. That is, the progeny line may have at least about 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99% genetic complement with the deposited line.

[0047] The "allele" is any one or more genes, all of which are associated with a characteristic or trait in a lettuce plant. In a diploid cell or organism, the pair of alleles of a given gene occupy corresponding loci on a pair of homologous chromosomes.

[0048] The genetic complementarity can be calculated, for example, by deciphering a molecular marker or a base sequence, comparing it with the molecular marker or base sequence of TEXLE203026, and calculating the coincidence rate. Examples of the molecular marker include SNP (single nucleotide polymorphism) markers, amplified fragment length polymorphism (AFLP) markers, restriction fragment length polymorphism (RFLP) markers, microsatellite markers, sequence-characterized amplified region (SCAR) markers, and cleaved amplified polymorphic sequence (CAPS) markers. Genome analysis methods using the molecular markers are well known and widely published (for example, References 4 and 5 below). The base sequence can be deciphered, for example, by extracting a chromosome from the progeny line and sequencing the chromosome. The proportion of alleles derived from the deposited line and the proportion of genetic complementarity may be estimated, for example, from the number of crosses. In this case, the proportion can be estimated from the number of crosses from the deposited line. As a specific example, when the number of crosses from the deposited line is n, the proportion can be estimated as, for example, (1 / 2)n×100%. Reference 4: Sinchan Adhikari et.al, “Application of molecular markers in plant genome analysis: a review”, The Nucleus, 2017, Volume 60, Issue 3, pp. 283-297 Reference 5: Elcio P. Guimaraes et.al., “MARKER-ASSISTED SELECTION Current status and future perspectives in crops, livestock, forestry and fish”, 2007, Springer, 29-49

[0049] The ratio of alleles and genetic complementarity derived from the deposited line is preferably, for example, an average value of the ratios of a plurality of progeny lines. The plurality is, for example, the number of individuals that can be statistically examined, specifically, 200 or more individuals, and preferably 200 to 1000 individuals.

[0050] The progeny line may have polymorphisms of the molecular markers derived from the deposited line. The deposited line may, for example, have at least about 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of its molecular markers derived from the deposited line. That is, the progeny line may have at least about 6.25%, 12.5%, 25%, 30%, 35%, 40%, 45%, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% of the molecular markers identical to the deposited line. In the present invention, when, for example, 50%, 55%, 60%, 65%, 70%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% or more of the molecular markers of a target lettuce plant match those of the molecular markers of the deposited line, the target lettuce plant can be determined (distinguished, presumed, identified, or differentiated) to be a descendant line of the deposited line.

[0051] The progeny line and the lettuce line TEXLE203026 may have, for example, a mutation or an introduced gene. In this case, the progeny line has, for example, one, two, three, or four or more traits or introduced genes introduced therein, and the traits are modified accordingly. The progeny line can be produced, for example, by introducing a mutation or an introduced gene into the deposited line or its progeny line. The mutation may be artificially introduced or naturally introduced, and the introduction of an artificial mutation can also be referred to as induction of a mutation. The mutation may be, for example, a chemical-induced mutation or a radiation-induced mutation. The mutation may be, for example, introduced by a molecular biology technique or a genome editing technique (for example, Reference 6 below). The mutation or introduced gene may also be introduced into lettuce seeds, lettuce plants, cell cultures, and / or tissue cultures of the deposited line. The introduced gene can be, for example, a method using Agrobacterium tumefaciens. Reference 6: Yanfei Mao et.al., “Gene editing in plants: progress and challenges”, National Science Review, 2019, vol. 6, pp. 421-437

[0052] The one or more traits include, for example, resistance to downy mildew (Bl16 to 37, Bl5 to 11US), resistance to root rot race 2, resistance to bacterial rot, resistance to corky root, resistance to lettuce mosaic virus, resistance to aphids, resistance to leafminers, and resistance to browning of cut surfaces.

[0053] The transgene refers to a desired gene introduced into the genome of a plant, for example, by genetic engineering techniques or traditional breeding methods. The transgene may include, for example, a gene introduced by single locus conversion. The transgene may be derived, for example, from the same species or from a different species. The transgene may be a gene containing the same base sequence as the species of origin or a gene containing a different base sequence. In the latter case, the different base sequence can be prepared, for example, by performing codon optimization, addition of a transcriptional regulator such as a promoter, or the like, on the same base sequence. The transgene may include a translation region and a non-translation region.

[0054] <Haploid and doubled haploid plants> The lettuce plants of the invention may be haploid and / or doubled haploid plants obtained, obtainable or derived from said deposited line. The haploid and / or doubled haploid plants of said deposited line may be used in a method of producing a parent line of said deposited line. In one embodiment, the invention may provide a plant of a haploid and / or doubled haploid plant, a plant part of a haploid and / or doubled haploid plant, or a seed of a haploid and / or doubled haploid plant.

[0055] The doubled haploid plant can be produced by doubling chromosomes in a haploid plant or cell (see, for example, Reference 7 below). As a specific example, haploid pollen or ovules are cultured under specific conditions to form plantlets with 1n chromosomes. The plantlets are then treated with a chemical substance such as colchicine to double the chromosomes. As a result, the cells of the plantlets have 2n chromosomes (doubled haploid). The doubled haploid plant and its progeny can be obtained by growing the plantlets after the treatment. Reference 7: Jim M. Dunwell, “Haploids in flowering plants: origins and exploitation”, Plant Biotechnology Journal, 2010, volume 8, pp. 377-424

[0056] <How to make lettuce plants> As described above, the method for producing a lettuce plant of the present invention includes a crossbreeding step of crossbreeding a first lettuce plant with a second lettuce plant, the first lettuce plant being the lettuce plant of the present invention. The production method of the present invention is characterized in that the lettuce plant of the present invention is used as at least one of the parents in the crossbreeding step, and other steps and conditions are not particularly limited.

[0057] The method for producing a lettuce plant of the present invention further comprises a selfing step of self-pollinating the lettuce plant of the present invention. The production method of the present invention is characterized by self-pollinating the lettuce plant of the present invention, and other steps and conditions are not particularly limited.

[0058] According to the production method of the present invention, progeny lines of the deposited line can be produced. The explanation of the production method of the present invention can be applied to the lettuce plant of the present invention.

[0059] In the present invention, the cross between the first lettuce plant (first parent line) and the second lettuce plant (second parent line) may be, for example, a cross between the same individuals (normal self-pollination), a cross between individuals of the same clone or between individuals of a line maintained as an inbred line (quasi-self-pollination), or a cross between different individuals (cross-pollination). In the case of normal self-pollination, for example, one of the first parent line and the second parent line is a female organ of the same individual, and the other is pollen of the same individual.

[0060] The seeds produced by crossing (cross-pollination) between said different individuals, and the plants obtained by growing the seeds, comprise first generation hybrid F1 (Hybrid First Generation, F1 hybrid).

[0061] In the present invention, the first parent line is the lettuce plant of the present invention, for example, the lettuce plant deposited under the aforementioned accession number FERM BP-22478 or a progeny line thereof.

[0062] The second parent line is not particularly limited, and any lettuce plant can be used. The second parent line may be, for example, a lettuce plant of the same taxonomic species as the first parent line, or a lettuce plant of a different species. The second parent line may be, for example, the deposited line or a progeny line, or another lettuce plant.

[0063] The production method of the present invention may further include, for example, a growing step of growing a progeny line obtained in the crossbreeding step after the crossbreeding step. Examples of the growing conditions in the growing step include general growing conditions for lettuce plants.

[0064] The lettuce plant of the present invention can be obtained, for example, by the production method of the present invention.

[0065] <How to produce lettuce seeds> The present invention provides a method for producing lettuce seeds. The method for producing lettuce seeds of the present invention comprises the steps of selfing or crossing a lettuce plant of the deposited line with another lettuce plant, and optionally producing (collecting or harvesting) the resulting seeds. The method for producing seeds of the present invention may also provide a plant, plant part or seed by growing a seed of the lettuce plant.

[0066] The seed production method of the present invention may be a method for producing seeds derived from the deposited line. In this case, the seed production method of the present invention may include a step of (a) crossing the plant of the deposited line with another lettuce plant to produce seeds. The seed production method of the present invention may further include a step of (b) cultivating a lettuce plant from the seed of the step (a) to produce a lettuce plant derived from the deposited line, and a step of (c) self-pollinating or crossing the lettuce plant of the step (b) with another lettuce plant to produce an additional lettuce plant derived from the deposited line. Furthermore, the seed production method of the present invention may (d) optionally repeat the steps (b) and (c) one or more times to produce an additional lettuce plant derived from the deposited line. In this case, the additional lettuce plant obtained in the step (c) can be used as the lettuce plant cultivated from the seed of the step (a) in the step (b). The "one or more times" may be, for example, 1 to 10 times, 3 to 7 times, or 3 to 5 times. The seed production method of the present invention may further include a step of collecting or harvesting the seeds. The seed production method of the present invention may provide seeds produced by the above method, and plants or plant parts obtained by growing the seeds.

[0067] <Method of producing hybrid lettuce plants> The present invention provides a method for producing a hybrid lettuce plant. The method for producing a hybrid plant of the present invention comprises the step of crossing a lettuce plant of the present invention with another lettuce plant. The method for producing a hybrid plant of the present invention may comprise the step of collecting or harvesting seeds obtained by the cross. The method for producing a hybrid plant of the present invention may also provide seeds and hybrid plants or parts of hybrid plant individuals produced by the above methods.

[0068] <Method of introducing new traits> The present invention provides a method for introducing at least one new characteristic or trait (hereinafter, collectively referred to as "trait") into the deposited line. The method for introducing a trait of the present invention can also be referred to as, for example, a method for producing a lettuce plant into which a new trait has been introduced. The method for introducing a trait of the present invention includes, for example, (a) a step of crossing a plant of the deposited line with a lettuce plant containing at least one new trait to produce a progeny line, and (b) a step of selecting a progeny line containing at least one new trait. The method for introducing a trait of the present invention includes, for example, a step of (c) a step of crossing the progeny line with the deposited line to produce backcross progeny seeds, and (d) a step of selecting a backcross progeny containing at least one new trait and essentially all physiological and morphological characteristics of the deposited line. In the steps (b) and (d), the selection of a progeny line having a new trait may be carried out, for example, by detecting the trait, or by detecting a gene or molecular marker associated (linked) with the trait. The new traits include resistance to downy mildew (Bl16-37, Bl5-11US), resistance to race 2 root rot fungus, resistance to bacterial rot disease, resistance to corky root disease, resistance to lettuce mosaic virus disease, resistance to aphids, resistance to leafminers, resistance to browning of cut surfaces, etc. The introduction of a new trait by the above method may be defined as single locus conversion.

[0069] The method for introducing a trait of the present invention may (e) optionally repeat the steps (c) and (d) one or more times to produce a lettuce plant containing at least one new trait. The lettuce plant containing the new trait may be defined as a lettuce plant containing at least one single locus conversion. In this case, the method for introducing a trait of the present invention may use the backcross progeny selected in the step (d) as the progeny line in the step (c). The lettuce plant obtained or obtainable in the step (e) may have essentially all of the physiological and morphological characteristics of the deposited line. The "essentially all of the physiological and morphological characteristics" may be used in the explanation of the progeny line by replacing "progeny line" with "lettuce plant obtained or obtainable in the step (e)". The "one or more times" may be, for example, 1 to 10 times, 3 to 7 times, or 3 to 5 times. The method for introducing a trait of the present invention may include a step of collecting or harvesting seeds. The method for introducing a trait of the present invention may provide seeds produced by the above method, and plants or parts of plants obtained by growing the seeds.

[0070] <Method of introducing transgene> The present invention provides a method for producing a plant derived from the deposited line, the plant containing at least one new characteristic or trait. The method for introducing a transgene of the present invention can also be referred to as a method for producing a lettuce plant having a new trait introduced therein.

[0071] The method for introducing a transgene of the present invention includes, for example, a step of introducing a mutation or transgene that confers at least one new trait into a plant of a deposited line. The introduction of the mutation or transgene can be carried out, for example, in the same manner as the introduction of a mutation or transgene in the progeny line. The lettuce plant obtained or obtainable by the introduction step may have essentially all of the physiological and morphological characteristics of the deposited line. The "essentially all of the physiological and morphological characteristics" can be used in the description of the progeny line by replacing "progeny line" with "lettuce plant obtained or obtainable by the introduction step". The method for introducing a transgene of the present invention may include a step of collecting or harvesting seeds. The method for introducing a transgene of the present invention may provide seeds produced by the above method, and parts of plants or plant individuals obtained by growing the seeds. Examples of the new traits include resistance to downy mildew (Bl16-37, Bl5-11US), resistance to root rot race 2, resistance to bacterial rot, resistance to corky root, resistance to lettuce mosaic virus, resistance to aphids, resistance to leafminers, and resistance to browning of cut surfaces.

[0072] <Lettuce plant regeneration and regeneration method> The invention provides lettuce plants regenerated from cell cultures, tissue cultures, or protoplasts of the deposited line (hereinafter referred to as "regenerates"). The invention may also provide cell or tissue cultures of regenerable cells, or protoplasts derived from the deposited line of lettuce plants. The cells, tissues, or protoplasts may be derived from tissues including leaves, pollen, embryos, cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds, or stems.

[0073] The present invention provides a method for the propagation or multiplication of a lettuce plant of the deposited line. The propagation of the lettuce plant of the deposited line may be vegetative propagation of the lettuce plant of the deposited line. In this case, the regeneration method of the lettuce plant of the present invention includes, for example, (a) collecting tissue capable of propagation from the plant of the deposited line, (b) culturing the tissue to obtain a propagated shoot, and (c) rooting the propagated shoot to obtain a rooted plantlet. The regeneration method of the lettuce plant of the present invention may further include (d) optionally growing a plant from the rooted plantlet. For the vegetative propagation method, see, for example, References 8 and 9 below. The regeneration method of the present invention may provide, for example, a plantlet, plant or part of a plant individual regenerated (produced) by the above method. The plant may have essentially all of the physiological and morphological characteristics of the deposited line. The above-mentioned "essentially all physiological and morphological characteristics" can be used in the description of the progeny line by replacing "progeny line" with "regenerated plant." Reference 8: Habtamu Gudisa Megersa, “Propagation Methods of Selected Horticultural Crops by Specialized Organs: Review”, Journal of Horticulture, 2017, Volume 4, Issue 2, 1000198 Reference 9: Nitish Kumar et.al., “In vitro Plant Propagation: A Review”, Journal of Forest Science, 2011, Vol. 21, No. 2, pp. 61-72

[0074] <Harvested and processed products of lettuce plants> The present invention provides harvested and / or processed products of the deposited line or progeny line, the harvested products being whole plants or parts of individual plants, preferably including leaves (e.g., head leaves and expanded leaves), leaves and roots, or seeds.

[0075] The processed product includes any product obtained by processing the deposited line or progeny line. The processing is not particularly limited, and examples thereof include cutting, slicing, grounding, pureeing, drying, canning, bottling, washing, packaging, freezing and / or heat treatment. In the deposited line or progeny line, the part of the plant or plant individual used for the processed product is, for example, a leaf. The processed product may be, for example, the deposited line or progeny line that has been washed and packaged. The processed product may be, for example, housed in a container of any size or shape. Specific examples of the container include a bag, a box, a carton, and the like.

[0076] The invention may provide a container containing one or more lettuce plants, said container containing a whole plant or parts of an individual plant.

[0077] The present invention may provide a method for producing a lettuce plant as a food product (a method for producing a food product). The method for producing a food product of the present invention may, for example, comprise a step of collecting or harvesting a whole plant or a part of a plant individual of the deposited line or a progeny line, preferably a leaf of the deposited line or a progeny line. The method for producing a food product of the present invention may also comprise a step of cultivating a lettuce plant of the deposited line or a progeny line until it matures.

[0078] <Method of determining genotype> The present invention provides a method for determining or detecting the genotype of a deposited line or a progeny line. The genotype determination method of the present invention includes, for example, (a) a step of obtaining a nucleic acid sample from the deposited line or a progeny line, and (b) a step of detecting a genome in the nucleic acid sample. In the step (a), the method for preparing nucleic acid from the deposited line or the progeny line can be performed using a general method for preparing nucleic acid from tissue. In the step (b), for example, polymorphisms and / or alleles in the genome in the nucleic acid sample are detected. The detection of the polymorphism and / or allele can be carried out using, for example, SNP (single nucleotide polymorphism) genotyping, amplified fragment length polymorphism detection (AFLP), restriction fragment length polymorphism discrimination (RFLP) of genomic DNA, sequence-characterized amplified region detection (SCAR) of genomic DNA, cleaved amplified polymorphic sequence detection (CAPS) of genomic DNA, random amplified polymorphic detection (RAPD) of genomic DNA, polymerase chain reaction (PCR), DNA sequence, allele specific oligonucleotide (ASO) probe, or DNA microarray. The detection of the polymorphism and / or allele may be carried out, for example, by sequencing the base sequence of the genome, or may be carried out with reference to the SNP of the deposited lineage as described above. In the step (b), one polymorphism and / or allele in the genomic DNA may be detected, or two or more polymorphisms and / or alleles may be detected. The genotyping method of the present invention may include a step of storing the detection results of the polymorphisms and / or alleles in a computer readable medium. The present invention may provide a computer readable medium produced by such a method.

[0079] The genotype determination method of the present invention may be carried out on any lettuce plant (target lettuce plant) instead of the deposited line or the progeny line. In this case, the genotype determination method of the present invention may further include, for example, a step of determining whether the target lettuce plant is the progeny line based on the result of step (b). The determination may also be referred to as, for example, discrimination, estimation, appraisal, or identification. The determination may be made based on, for example, the rate of agreement between the result of step (b) and the genotype of the deposited line. EXAMPLES

[0080] The present invention will be described in detail below using examples, but the present invention is not limited to the embodiments described in the examples.

[0081] (1) Breeding of the deposited strain In 2015, a lettuce line with resistance to root rot race 1 was crossed with a lettuce line with early maturity, excellent bulb enlargement (large bulbs), and late bolting, and seeds of the F1 generation were obtained. In 2016, the F1 generation was cultivated and self-fertilized seeds were obtained. In 2017, 160 plants of the F2 generation were cultivated (80 plants x 2 cropping types), and 18 individuals with strong plant vigor, excellent bulb enlargement, late bolting, and resistance to root rot race 1 were selected and harvested. In 2018, the F3 generation was cultivated, and individuals with strong plant vigor, excellent bulb enlargement, late bolting, and resistance to root rot race 1 were selected and harvested. Thereafter, selection and seed harvesting were repeated in the same manner, and in 2022, when the F7 generation was reached, it was determined that the desired traits had been fixed, and cultivation was terminated.

[0082] A total of about 2,000 F7 plants were cultivated in a field in Yuma, Arizona, USA in 2022 in four crop types with sowing in September, and it was confirmed that there was no variation in characteristics within the lineage, and that the bred lettuce varieties had uniformity and stability. Then, seeds of three lines of the F7 generation were mixed and named TEXLE203026, and a part of it was deposited under the accession number FERM BP-22478.

[0083] (2) Characteristics of the deposited strain The characteristics and traits of the plant individuals of the deposited lines were evaluated according to the Ministry of Agriculture, Forestry and Fisheries' examination criteria for variety registration. The results are shown in Table 2.

[0084] [Table 2]

[0085] Although the present invention has been described above with reference to the embodiments and examples, the present invention is not limited to the above-mentioned embodiments and examples. Various modifications that can be understood by those skilled in the art can be made to the configuration and details of the present invention within the scope of the present invention. [Industrial Applicability]

[0086] As described above, the present invention provides a novel lettuce plant, which is extremely useful in the agricultural field, for example, in breeding. <Additional Notes> Some or all of the above-described embodiments and examples may be described as follows, but are not limited to the following: (Appendix 1) A lettuce plant, including the lettuce plant identified by accession number FERM BP-22478 or a progeny thereof. (Appendix 2) The progeny line is a lettuce plant that contains 50% or more of the alleles of the lettuce plant identified by accession number FERM BP-22478. (Appendix 3) The progeny line contains 50% or more alleles of the lettuce plant identified by accession number FERM BP-22478; The progeny line is a lettuce plant having the following traits (1) to (3): (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant.” (Appendix 4) The progeny line contains 50% or more alleles of the lettuce plant identified by accession number FERM BP-22478; The progeny line is a lettuce plant having the following traits (1) to (13): (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant”; (4) The seed color is "white"; (5) The width of the stock is "medium"; (6) The upper leaves of the plant have a strong overlap; (7) The number of leaf lobes is absent or very small; (8) The leaf shape is "narrow oblate"; (9) Anthocyanin coloration of leaves is absent or very weak; (10) The leaf irregularities are "weak"; (11) The leaf margins are somewhat wavier; (12) The depth of the incision on the edge of the leaf is "slightly deep"; (13) The cross-sectional shape of the leaf is "oblate." (Appendix 5) 5. The lettuce plant according to any one of claims 1 to 4, wherein the lettuce plant is a plant body or a part thereof. (Appendix 6) 6. The lettuce plant of any one of claims 1 to 5, wherein the lettuce plant is a seed. (Appendix 7) A method for producing a lettuce plant, comprising a crossbreeding step of crossing a first lettuce plant with a second lettuce plant, wherein the first lettuce plant is a lettuce plant according to any one of appendices 1 to 6. (Appendix 8) seeds of lettuce cultivar TEXLE 203026, a representative sample being seeds of the lettuce plant deposited under accession number FERM BP-22478. (Appendix 9) A lettuce plant of the lettuce variety TEXLE203026, a representative sample being a seed of the lettuce plant deposited under accession number FERM BP-22478. (Appendix 10) 10. A lettuce plant or part thereof, wherein the lettuce plant or part thereof has essentially all of the physiological and morphological characteristics of the lettuce plant of claim 9. (Appendix 11) A progeny lettuce plant of the lettuce plant according to Supplementary Note 9, wherein the progeny lettuce plant contains at least 50% of the alleles of the lettuce plant according to Supplementary Note 9, and the progeny lettuce plant has the following traits (1) to (13): (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant”; (4) The seed color is "white"; (5) The width of the stock is "medium"; (6) The upper leaves of the plant have a strong overlap; (7) The number of leaf lobes is absent or very small; (8) The leaf shape is "narrow oblate"; (9) Anthocyanin coloration of leaves is absent or very weak; (10) The leaf irregularities are "weak"; (11) The leaf margins are somewhat wavier; (12) The depth of the incision on the edge of the leaf is "slightly deep"; (13) The cross-sectional shape of the leaf is "oblate." (Appendix 12) 12. A seed producing the lettuce plant of claim 11. (Appendix 13) 10. A part of a lettuce plant as described in Appendix 9. (Appendix 14) 14. A plant part according to claim 13, wherein the plant part comprises a microspore, pollen, ovary, ovule, embryo sac, egg cell, cutting, root, stem, leaf, cell or protoplast. (Appendix 15) 10. A method for producing lettuce seeds, the method comprising selfing or crossing the lettuce plant of claim 9 with another lettuce plant, and harvesting the resulting seeds (seed harvesting). (Appendix 16) 16. A lettuce seed derived from a lettuce plant, produced by the method of claim 15. (Appendix 17) 17. A lettuce plant or part thereof produced by growing the lettuce seed of claim 16. (Appendix 18) A lettuce plant or part thereof according to Supplementary Note 17, wherein the lettuce plant or part thereof contains at least 50% alleles of the lettuce variety TEXLE203026, a representative sample of which is a seed of the lettuce plant deposited under Accession No. FERM BP-22478, and the lettuce plant or part thereof has the following traits (1) to (13): (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant”; (4) The seed color is "white"; (5) The width of the stock is "medium"; (6) The upper leaves of the plant have a strong overlap; (7) The number of leaf lobes is absent or very small; (8) The leaf shape is "narrow oblate"; (9) Anthocyanin coloration of leaves is absent or very weak; (10) The leaf irregularities are "weak"; (11) The leaf margins are somewhat wavier; (12) The depth of the incision on the edge of the leaf is "slightly deep"; (13) The cross-sectional shape of the leaf is "oblate." (Appendix 19) 18. The lettuce plant or part thereof of claim 17, wherein the lettuce plant or part thereof has essentially all of the physiological and morphological characteristics of lettuce cultivar TEXLE 203026, a representative sample of which is a seed of the lettuce plant deposited under accession number FERM BP-22478. (Appendix 20) 19. The lettuce plant or part thereof of claim 18, wherein the lettuce plant or part thereof has been modified in one or more traits. (Appendix 21) 21. The lettuce plant or part thereof of claim 20, wherein the modification is achieved by mutagenesis. (Appendix 22) 10. A method for producing seeds of a lettuce plant derived from the lettuce plant of claim 9, the method comprising: (a) crossing lettuce variety TEXLE 203026, which is a seed of the lettuce plant deposited under accession number FERM BP-22478, with another lettuce plant to produce seed; (b) growing lettuce plants from the seeds of step (a) to produce lettuce plants derived from the lettuce variety TEXLE203026; (c) selfing or crossing the lettuce plant of step (b) with another lettuce plant to produce an additional lettuce plant derived from lettuce variety TEXLE203026; (d) optionally repeating steps (b) and (c) one or more times to produce additional lettuce plants derived from the lettuce variety TEXLE203026, wherein the lettuce plants in step (b) are grown from the additional lettuce plants of step (c). (Appendix 23) A seed produced by the method according to claim 22, the seed containing at least 50% of the alleles of the lettuce plant according to claim 9, and a lettuce plant grown from the seed having the following traits (1) to (13): (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant”; (4) The seed color is "white"; (5) The width of the stock is "medium"; (6) The upper leaves of the plant have a strong overlap; (7) The number of leaf lobes is absent or very small; (8) The leaf shape is "narrow oblate"; (9) Anthocyanin coloration of leaves is absent or very weak; (10) The leaf irregularities are "weak"; (11) The leaf margins are somewhat wavier; (12) The depth of the incision on the edge of the leaf is "slightly deep"; (13) The cross-sectional shape of the leaf is "oblate." (Appendix 24) 24. A lettuce plant produced by growing a seed of the lettuce plant of claim 23. (Appendix 25) A method for introducing at least one new trait into a lettuce plant according to claim 9, The method comprises: (a) crossing lettuce cultivar TEXLE203026, which is a seed of the lettuce plant deposited under accession number FERM BP-22478, with a lettuce plant having at least one new trait to produce progeny; (b) selecting progeny containing at least one new trait; (c) crossing the progeny with lettuce variety TEXLE203026 to produce a backcross progeny; (d) selecting a backcross progeny having essentially all of the physiological and morphological characteristics of the lettuce variety TEXLE 203026, including at least one new trait; (e) optionally repeating steps (c) and (d) one or more times to produce a lettuce plant containing at least one new trait and having essentially all of the physiological and morphological characteristics of lettuce variety TEXLE203026, wherein the lettuce plant in step (c) is a selected backcross progeny of step (d). (Appendix 26) A lettuce plant produced by the method of claim 25. (Appendix 27) 1. A method for producing a lettuce plant derived from lettuce variety TEXLE203026 that contains at least one new trait, the method comprising introducing a mutation or transgene that confers the at least one trait into lettuce variety TEXLE203026, which is a seed of the lettuce plant deposited under accession number FERM BP-22478. (Appendix 28) A lettuce plant produced by the method of claim 27. (Appendix 29) 10. A method for producing lettuce head leaves as a food product, the method comprising harvesting the head leaves or the head leaves, expanded leaves and stems of a lettuce plant according to claim 9. (Appendix 30) 10. A processed product of the lettuce plant of claim 9, said processed product comprising cut, sliced, ground, pureed, dried, canned, bottled, washed, packaged, frozen and / or heat treated heads. (Appendix 31) A method for determining the genotype of a lettuce plant or a progeny thereof according to claim 9, comprising: (a) obtaining a nucleic acid sample from a lettuce plant or a progeny thereof as described in Appendix 9; (b) detecting a polymorphism in the nucleic acid sample; The method includes: (Appendix 32) A regenerable cell or protoplast tissue culture derived from the lettuce plant of claim 9. (Appendix 33) 33. The cultured tissue of claim 32, wherein the cells or protoplasts are derived from leaves, pollen, embryos, cotyledons, hypocotyls, meristematic cells, roots, root tips, anthers, flowers, seeds or stems. (Appendix 34) 34. A lettuce plant regenerated from the cultured tissue described in appended claim 33. (Appendix 35) 35. The lettuce plant of claim 34, wherein the lettuce plant has essentially all of the physiological and morphological characteristics of lettuce cultivar TEXLE 203026, which is a seed of the lettuce plant deposited under accession number FERM BP-22478. (Appendix 36) 10. A method for vegetatively propagating a lettuce plant according to claim 9, comprising: (a) recovering reproductive tissue from a lettuce plant of the lettuce variety TEXLE 203026, which is the seed of the lettuce plant deposited under accession number FERM BP-22478; (b) culturing the tissue and obtaining proliferated shoots; (c) rooting the propagated shoots and obtaining rooted plantlets; (d) optionally growing plants from the rooted plantlets; The method includes: (Appendix 37) 37. A lettuce plantlet or plant produced by the method of appended claim 36, wherein the lettuce plantlet or plant has essentially all of the physiological and morphological characteristics of lettuce variety TEXLE 203026, which is a seed of the lettuce plant deposited under accession number FERM BP-22478. (Appendix 38) Lettuce plants, including the lettuce plant identified by accession number FERM BP-22478. (Appendix 39) Progeny of the lettuce plant of claim 38, The progeny line is a lettuce plant having the following traits (1) to (13): (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant”; (4) The seed color is "white"; (5) The width of the stock is "medium"; (6) The upper leaves of the plant have a strong overlap; (7) The number of leaf lobes is absent or very small; (8) The leaf shape is "narrow oblate"; (9) Anthocyanin coloration of leaves is absent or very weak; (10) The leaf irregularities are "weak"; (11) The leaf margins are somewhat wavier; (12) The depth of the incision on the edge of the leaf is "slightly deep"; (13) The cross-sectional shape of the leaf is "oblate." (Appendix 40) 40. A lettuce plant comprising a first generation hybrid line of the lettuce plant of claim 38 or 39. (Appendix 41) 41. The lettuce plant of any one of appendix 38 to 40, wherein the lettuce plant is a plant body or part thereof. (Appendix 42) 42. The lettuce plant of any one of claims 38 to 41, wherein the lettuce plant is a seed. (Appendix 43) A method for producing a lettuce plant, comprising a self-pollinating step of self-pollinating the lettuce plant according to any one of appendices 38 to 42. (Appendix 44) A method for producing a lettuce plant, comprising a crossbreeding step of crossbreeding the lettuce plant according to any one of appendices 38 to 42 with another lettuce plant. (Appendix 45) 45. A method for producing a lettuce plant according to claim 43 or 44, comprising a seed harvesting step.

Claims

1. 1. A seed of a lettuce plant, said seed being of the lettuce line TEXLE203026, a representative sample of said seed being deposited under accession number FERM BP-22478.

2. 2. A lettuce plant grown from the seed of claim 1.

3. A lettuce plant having essentially all of the physiological and morphological characteristics of the lettuce plant of claim 2.

4. A tissue culture of the lettuce plant of claim 2.

5. A lettuce plant regenerated from the tissue culture of claim 4.

6. 1. A lettuce plant of the lettuce line TEXLE203026, a representative sample of seeds of said plant being deposited under accession number FERM BP-22478, said plant further comprising a mutation and / or a transgene.

7. 1. A lettuce plant of lettuce line TEXLE203026, a representative sample of seeds of said plant being deposited under accession number FERM BP-22478, said plant further comprising a single locus conversion.

8. A progeny line of the lettuce plant according to claim 2, comprising at least 50% of the alleles of the lettuce line TEXLE203026 and having the following traits (1), (2) and (3): (1) The size of the ball is "medium"; (2) The start of bolting is in the late period; (3) Resistance to root rot race 1 is “highly resistant.”

9. A part of a lettuce plant according to any one of claims 2 to 8.

10. A hybrid first generation lettuce plant, or a part thereof, having the lettuce plant according to any one of claims 2 to 8 as a first parent line and another lettuce plant as a second parent line.

11. A method for producing a lettuce plant, comprising a self-pollinating step of self-pollinating the lettuce plant according to any one of claims 2 to 8.

12. A method for producing a lettuce plant, comprising a crossbreeding step of crossbreeding the lettuce plant according to any one of claims 2 to 8 with another lettuce plant.

13. A method for producing a lettuce plant, comprising introducing a mutation into the lettuce seed according to claim 1 and growing the seed.

14. A method for producing a lettuce plant, comprising introducing a mutation into the lettuce plant according to any one of claims 2 to 8.

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