Methods for improving glucosinolate content in brassicaceae vegetables and methods for producing glucosinolate-containing brassicaceae vegetables

Abstract

To provide a method for keeping the glucosinolate content of a Brassicaceae vegetable for a long period of time.SOLUTION: The method according to the invention for improving the glucosinolate content in a Brassicaceae vegetable comprises culturing a Brassicaceae vegetable for 30 days or longer with feed of a nutrient solution and irradiation of artificial light, where nutrient solution contains sulfuric ion at a concentration of 3000 ppm or more.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a method for increasing the glucosinolate content of cruciferous vegetables, A method for producing glucosinolate-containing cruciferous vegetables. [Background technology]

[0002] In recent years, what is required in the market is vegetables with health benefits. The plant in question is the shoots of a plant in the Cruciferae family, such as broccoli. These shoots are rich in glucosinolates. When nolate is metabolized, it becomes sulforaphane. The reason for this is the action of sulforaphane, which specifically has antioxidant, detoxifying, and anti-fat accumulation effects. For production, antibacterial properties, etc.

[0003] One such candidate for valuable vegetables is the cruciferous vegetables, because many This is because cruciferous vegetables contain glucosinolates. The metabolites of cosinolates have a variety of health benefits.

[0004] Few methods for increasing the glucosinolate content in cruciferous vegetables have been disclosed. Patent Document 6 discloses a method for cultivating sprouts, and the method The light emitting diode is used to illuminate the leafy vegetables under artificial lighting. This is a method for producing vegetables under artificial lighting. In order to solve the problem that the nutritional content of vegetables tends to be low, Just before harvesting, replace the nutrient solution with water and set the day length to 20 hours or more. The upper limit of the light intensity is 20,000 lux, and the plants are grown hydroponically for 2 to 5 days. This is the method.

[0005] In addition, Non-Patent Document 1 discloses the growth of cruciferous vegetables at a light intensity of ppf400. However, by increasing the light intensity, the amount of glucoraphanin and glucoraphenin The concentration of arachis hypoxia increased with increasing light intensity, whereas the concentration decreased with the number of days after leaf emergence, regardless of light intensity. is. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] International Publication WO97 / 09889 [Patent Document 2] JP2006-109754A [Patent Document 3] International Publication WO01 / 045661 [Patent Document 4] Patent Publication No. 2010-126489 [Patent Document 5] International Publication WO2002 / 015722 [Patent Document 6] Patent Publication No. 2007-075073 [Patent Document 7] International Publication WO2010 / 140632 [Non-patent literature]

[0007] [Non-Patent Document 1] Abstracts of the lecture given at the Japan Society for Bioenvironmental Engineering Conference: Effects of light intensity on glucosinolate content and growth of Raphanobrassica Naga-No. 48 Summary of the Invention [Problem to be solved by the invention]

[0008] The problem to be solved by the present invention is to improve the glucosinolates of cruciferous vegetables when they are cultivated. The aim is to improve the rate content. [Means for solving the problem]

[0009] Based on the above, the inventors of the present application have conducted extensive research and discovered that the sulfate ion concentration of the nutrient solution can be increased. The glucosinolate content of the plant is improved when cultivated. When vegetables are grown under artificial light for extended periods, their glucosinolate content does not improve or decreases. In other words, depending on the sulfate ion concentration of the nutrient solution, the light intensity and the light irradiation time, the absorption The glucosinolate content of the Lamiaceae vegetables is improved. From this viewpoint, the present invention is defined as follows: As follows:

[0010] The method for increasing the glucosinolate content of cruciferous vegetables according to the present invention comprises at least At the very least, the cultivation is carried out. In the cultivation, cruciferous vegetables are cultivated. In the cultivation, Cruciferous vegetables are given a nutrient solution. The sulfate ion concentration of the nutrient solution is 3000 ppm or more. In the cultivation, the cruciferous vegetables are irradiated with artificial light. The cultivation period is 30 days or more.

[0011] The method for producing glucosinolate-containing cruciferous vegetables according to the present invention comprises at least In both cases, cultivation is used. In cultivation, glucosinolate-containing cruciferous vegetables are grown. In the cultivation, a nutrient solution is applied to the glucosinolate-containing cruciferous vegetables. In the cultivation, the sulfate ion concentration is 3000 ppm or more. The cruciferous vegetables containing the phytoplankton are exposed to artificial light. The cultivation period is 30 days or more. be. Effect of the Invention

[0012] The present invention makes it possible to grow cruciferous vegetables in artificial light for long periods of time, and to improve their overall quality. The aim is to improve the cosinolate content. [Brief description of the drawings]

[0013] [Figure 1] Flow diagram of the method for increasing the glucosinolate content of cruciferous vegetables according to the present embodiment [Diagram 2] Flow diagram of a method for producing glucosinolate-containing cruciferous vegetables according to the present embodiment [Diagram 3] Average sulfate ion concentration in each category (ppm) [Figure 4] Relationship between sulfate ion concentration and glucosinolate content in all samples [Diagram 5] Appearance of each category of stock DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0014] <Glucosinolate> Glucosinolates are derivatives of glucose and amino acids that contain sulfur and nitrogen. Glucosinolates in the present invention are a group of organic compounds. For example, glucoraphanin (also called sulforaphane glucosinolate), sinii Glynn, Glucoercin, Glucobrassicin, Glucoraphenin, Glucoraphasatin , phenethyl glucosinolate, etc. In the present invention, glucoraphanin is particularly preferred. It is preferable that

[0015] <Cruciferous vegetables> Cruciferous vegetables are vegetables that belong to the Brassicaceae family. Examples of cruciferous vegetables include Brassica vegetables (Chinese cabbage, turnip, bok choy, and corn) Tuna, tuna, mizuna, cabbage, broccoli, cauliflower, brussels sprouts, kolla vegetables of the genus Radish (radish, etc.), vegetables of the genus Watercress (watercress etc.), Arugula genus vegetables (arugula, etc.), Mustard genus vegetables (white mustard, etc.), Vegetables of the Horseradish genus (horseradish, etc.) and Wasabi genus (wasabi, etc.) Cruciferous vegetables can be hybrids of any other vegetable that belongs to the Brassicaceae family. For example, intergeneric hybrids between genera in the Brassicaceae family (Brassica vegetable x Radish) Brassica vegetables x Nasturtium vegetables, Brassica vegetables x Scutellaria vegetables vegetables, etc.) and interspecific hybrids between species in the Brassicaceae family (B. oleracea × B.rap a etc.).

[0016] Glucosinolate-containing cruciferous vegetables are vegetables belonging to the Brassicaceae family that contain glucosinolates. It refers to those that contain cinolate.

[0017] <Glucosinolate content in cruciferous vegetables> The glucosinolate content of cruciferous vegetables is the glucosinolate content of cruciferous vegetables. The glucosinolate content of cruciferous vegetables is defined as the glucosinolate content of cruciferous vegetables as specified in the Unless otherwise stated, the amount refers to the content (mg) per fresh weight (100g).

[0018] <Improved glucosinolate content> In the present invention, the improvement of glucosinolate content means the cultivation of cruciferous vegetables under artificial light. When cultivated in a conventional manner, the glucosinolate content is increased compared to when cultivated in a conventional manner. Preferably, the glucosinolate content is 1. More than three times as much glucose as normal cultivation. This means that the amount of cinolate in the fruit is three times higher than that in the normal cultivation. This refers to a condition in which the glucosinolate content is five times or more higher than that of the normal condition. The method for analyzing the amount will be described later.

[0019] Normal cultivation refers to cultivation in which the sulfate ion concentration of the nutrient solution is not adjusted. By not adjusting the concentration, it is meant that no sulfate is added to the commercial nutrient solution formulation. Normal cultivation refers to cultivation in which a nutrient solution with a sulfate ion concentration of 360 ppm or less is used.

[0020] <Method for increasing glucosinolate content in cruciferous vegetables according to the present invention> FIG. 1 shows a method for increasing the glucosinolate content of cruciferous vegetables according to the present invention (hereinafter The method is mainly comprised of cultivation (S11). It is.

[0021] <Cultivation (S11)> In the cultivation, cruciferous vegetables are cultivated. This process does not exclude the Examples include fertilization, spraying pesticides, controlling pests and diseases, These include raking leaves and buds.

[0022] <Nutrient solution> In the cultivation (S11), cruciferous vegetables are given a nutrient solution. The means of giving the nutrient solution are as follows: Any known nutrient solution may be used, and is not particularly limited. The sulfate ion concentration of the nutrient solution is 3000 ppm. The upper limit of the sulfate ion concentration in the nutrient solution is not particularly limited as long as it does not have any adverse effect on the plant body. There is no need for nutrients other than sulfate ions in the nutrient solution, as long as the cruciferous vegetables grow. The nutrient solution contains at least nitrogen, Phosphate, potassium, calcium, magnesium, manganese, and iron.

[0023] The nutrient solution may be circulated as necessary. When the nutrient solution is circulated, the nutrient solution is sterilized or disinfected. Examples of methods for sterilizing or disinfecting the nutrient solution include ozone treatment and UV irradiation. The amount of nutrient solution to be given depends on the type of plant and The amount of nutrient solution may be adjusted appropriately depending on the growth medium, etc., and is not particularly limited. It is preferable to apply the nutrients continuously throughout the cultivation period, rather than once a day. More on this later.

[0024] <Artificial light> In cultivation (S11), cruciferous vegetables are exposed to artificial light. The stage may be any known type and is not particularly limited. The artificial light includes at least the plant The wavelength of light necessary for plant growth is light with a wavelength that has no adverse effect on plant growth. However, it is preferably 400 nm to 700 nm.

[0025] Examples of light sources include fluorescent lamps (FL), metal halide lamps (MHL), and high-pressure nato These include high powered sapphire lamps (HPSL), light emitting diodes (LEDs), etc. Light intensity is determined by the PPFD (Photosynthetically active radiant flux density).

[0026] The light intensity of the light irradiated during cultivation (S11) was 100 μmol m -2 ·s -1 That's all. Preferably, 400 μmol m-2 ·s -1 The upper limit of light intensity is set so that it will not have a detrimental effect on the plant body. There is no particular limitation as long as it has no effect. Preferably, it is 500 μmol m -2 ·s -1 The following is the result. The light exposure time is 12 hours or more per day. Preferably, the light exposure time is 24 hours per day (constantly). The light intensity and duration of exposure to light were found to improve the glucosinolate content of cruciferous vegetables. There is no particular limitation as to the light intensity, and it may be appropriately selected so as to improve the light intensity. By increasing the irradiation time, the glucosinolate content can be increased. Even if the light intensity is increased, the glucosinolate content can be improved. The preferred light intensity is 400 μmol m -2 ·s -1 If the light intensity is 100 μmol m -2 ·s -1 If this is the case, the exposure time should be 24 hours per day.

[0027] The cumulative illuminance of light irradiated during cultivation (S11) (μmol m -2 ·s -1 h) is the light intensity (μmol m -2 ·s -1 The daily cumulative illuminance of light is calculated by multiplying the light intensity by the number of hours of exposure (h). Preferably 2,400 μmol m -2 ·s -1 ·h or more, more preferably 4,800 µmol m -2 ·s -1 h or more, and more preferably 9,600 μmol m -2 · s -1 The upper limit of the daily integrated illuminance of light is 100% or more, especially if there is no adverse effect on the plant body. Not limited to.

[0028] The cumulative illuminance of light during the cultivation period is 1,728,000 μmol m -2 ·s -1 ·h or more and more preferably 3,456,000 μmol m -2 ·s -1 h or more, and Preferably 6,912,000 μmol m -2 ·s -1 h or more, most preferably , 16,128,000 μmol m -2 ·s -1 ·h or more. The product of light per cultivation period The upper limit of the calculated illuminance is not particularly limited as long as it does not have an adverse effect on the plant body.

[0029] <Cultivation method> In this method, the cultivation method may be any method capable of cultivating cruciferous vegetables, and there are no particular limitations. From the viewpoint of providing a nutrient solution, the cultivation method is preferably a nutrient solution cultivation. Cultivation refers to a method of cultivation that uses a liquid that contains nutrients necessary for plant growth. Specific examples of cultivation methods include hydroponics, solid medium cultivation, and spray culture. The cultivation is preferably hydroponic. Hydroponic cultivation is a method in which part or all of the roots of the plant to be cultivated are cultured in a hydroponic medium. This refers to a cultivation method in which the plants are submerged in a nutrient solution.

[0030] <Cultivation location> In this method, the cultivation site is not particularly limited as long as it is possible to cultivate cruciferous vegetables. Examples include farm fields, vinyl greenhouses, and plant factories. Preferably, the plant factory is a plant factory.

[0031] <Cultivation period> In this method, the cultivation period is 30 days or more. (i.e., synonymous with planting date) to the end of cultivation (i.e., synonymous with harvest date). The cultivation period is preferably 30 days or more. Also, preferably, the cultivation period is 40 days or more. The cultivation period is 50 days or more, and more preferably 60 days or more and 150 days or less. It is not advisable to leave the stems of cruciferous vegetables for more than 150 days, as this will cause the stems to become woody and hard.

[0032] <Production method according to the present invention> FIG. 2 shows the flow of the production method according to the present invention (hereinafter referred to as the "production method"). This production method mainly consists of planting (S21), cultivation (S22), and harvesting (S23). ).

[0033] <Planting (S21)> Plant seedlings of cruciferous vegetables containing glucosinolates. The means for obtaining seedlings of the vegetable family is not particularly limited, and the seedlings may be distributed in the form of seedlings. It may be obtained from seeds that are distributed in the market, or from tissue culture, cuttings, etc. It may be obtained by vegetative propagation. In the case where seedlings are obtained by germinating seeds, If necessary, seeds may be sown before planting. Seeds can be sown directly in the area where cruciferous vegetables are grown, then thinned out and the remaining seedlings can be used as seedlings. When direct sowing, glucosinolate-containing cruciferous vegetables should be grown until the primary leaves reach approximately 5 cm. The time when the plant grows is the "planting date." The planting date is the date when the plant grows. There are no particular limitations as long as the time allows cultivation of rate-containing cruciferous vegetables.

[0034] <Cultivation (S22)> The cultivation (S22) in this production method is similar to the cultivation (S11) in this method.

[0035] <Harvest (S23)> In the harvesting step, glucosinolate-containing cruciferous vegetables are harvested. The means for harvesting cruciferous vegetables is not particularly limited as long as it is a known method. The glucosinolate content of selected glucosinolate-containing cruciferous vegetables was The amount of the russianthus-containing vegetable is not particularly limited because it varies depending on the type of the vegetable. However, it is preferable that the amount of the russianthus-containing vegetable is 30 mg / 100g or more, more preferably 60 mg / 100g or more, and even more preferably Preferably, it is 90 mg / 100 g or more, and most preferably, it is 120 mg / 100 g or more. The higher the glucosinolate content per unit weight, the more effective the food will be, even at low intakes. This allows for efficient intake of glucosinolates.

[0036] The size of the glucosinolate-containing cruciferous vegetables harvested varies depending on the intended use. When harvested leaves are distributed, the size of the leaves is small from the viewpoint of handling. It is preferable that the size is uniform.

[0037] The hardness of the leaves of harvested glucosinolate-containing cruciferous vegetables varies depending on the intended use. If the intended use is for fresh food, soft foods are preferred. If the purpose is to produce a processed food, softness is not an issue. EXAMPLES

[0038] The present invention will now be described in more detail with reference to exemplary embodiments. The description of examples is not intended to be limiting.

[0039] <Test 1: Effect of sulfate ion concentration in nutrient solution on glucosinolate content> <Cultured seedlings> An intergeneric hybrid with kale as the pollen parent and radish as the stigma parent (variety registration application number 33291 The name of the applied variety: Sainte Vert 48) was cultivated and raised in the Kagome Co., Ltd. research laboratory. Cultured seedlings were used for the test. The seedlings used for the test had developed to the fifth leaf (the fifth leaf). The plant height was about 15 cm. The cultured seedlings were planted on May 28, 2018, and each test Four strains were tested for each category.

[0040] <Cultivation> In categories 1-1 to 1-4, the cultured seedlings were incubated in an artificial climate chamber (manufactured by Nippon Medical Equipment Manufacturing Co., Ltd.) for 8 hours. The plants were grown in a 1000L container. The cultivation was carried out in a nutrient solution with the basic composition of Otsuka A formulation. (OAT Agrio Co., Ltd.) was used. For categories 1-2 to 4, commercially available magnesium sulfate was used. Specifically, the sulfate ion concentration of the nutrient solution was increased by using a 1200ppm (category 1-2), 1800ppm (category 1-3), and 3000ppm, respectively. (Category 1-4). In Category 1-1, the sulfate ion concentration of the nutrient solution was not adjusted. The composition of the nutrient solution for each group is shown in Table 1. The cultivation temperature in the artificial climate chamber was 25°C during the day and 15°C at night, and the cultivation humidity was 70%. Using LEDs (high-intensity LEDs for plant growth "Plant Fleck" manufactured by Nippon Medical and Chemical Equipment Co., Ltd.) The photoperiod was a 15-hour light / 9-hour dark cycle.

[0041] [Table 1]

[0042] <Analysis method> Cultivation was carried out until 60 days after planting, and the number of expanded leaves per plant, the maximum leaf length, the sulfate ion concentration, and the glucosinolate content were measured.

[0043] <Measurement of glucosinolate content> The measurement of the glucosinolate content was carried out by the following method. As the measurement site, one leaf with a size of 20 cm in diameter was collected from each plant, and the leaf blade was used. When there was more than one leaf with a size of 20 cm in diameter, the newer leaf was used. The measurement was repeated for 4 plants, and the average value was calculated. The measurement sample was crushed into a paste after inactivating the enzyme (myrosinase) with hot water, and extracted in a water bath set at 100℃ for 30 minutes, and then centrifuged at 10,000×g for 10 minutes at 4 ℃. The supernatant after centrifugation was filtered through a 0.2μm filter and quantified by HPLC. The detailed measurement conditions for HPLC are as follows.

[0044] <HPLC measurement conditions> Apparatus: ACQUITY UPLC H-Class system (manufactured by Waters) Column: ACQUITY CSH C18 (Φ2.1×100mm, 1.7μm) (manufactured by Waters) Column temperature: 30℃ Sample injection volume: 10μL Mobile phase A: ultrapure water: trifluoroacetic acid = 99.95:0.05 (v:v) Mobile phase B: methanol: trifluoroacetic acid = 99.95:0.05 (v:v) Gradient: Maintain the proportion of mobile phase B at 0% for 5 minutes Linear gradient from 0 to 10% of mobile phase B in 10 minutes Linear gradient from 10 to 100% of mobile phase B in 5 minutes Maintain the proportion of mobile phase B at 100% for 5 minutes Linear gradient from 100% to 0% mobile phase B in 2 minutes Maintain 0% mobile phase B for 5 minutes Flow rate: 0.1mL / min Detection wavelength: 235 nm

[0045] <Measurement of sulfate ion concentration> The sulfate ion concentration was measured by the following method. The measurement site was a 20 cm leaf from each plant. When there was more than one leaf of 20 cm in size, the newer leaf was used. The measurements were repeated for four plants, and the average value was calculated.

[0046] The measurement sample was crushed in a mixer until it became a paste. m Filter with a disposable syringe filter and quantify using ion chromatography. The detailed measurement conditions for the ion chromatograph are as follows:

[0047] <Ion chromatograph measurement conditions> Equipment: Ion chromatograph (Dionex Corporation) Column: Column for ion analysis (AS9: manufactured by Dionex Corporation) Sample injection volume: 25μL Mobile phase: 1.8 mM sodium carbonate / 1.7 mM sodium bicarbonate Flow rate: 1.0ml / min Detection: Electrical conductivity meter (50mA)

[0048] <Growth Survey> <Number of deployments> The number of expanded leaves was determined by counting all the leaves on each plant.

[0049] <Maximum leaf length> The maximum leaf length was measured from the tip to the base of the largest leaf on each plant using a tape measure. It was measured.

[0050] <Result> <Number of expanded leaves, maximum leaf length> The number of expanded leaves and maximum leaf length are shown in Table 2. The following can be seen from Table 2. The number of expanded leaves is, Comparing category 1-1 and category 1-2-4, the number of leaves was slightly higher in category 1-1. There was no difference between Category 1-1 and Category 1-2 to 1-4. It can be concluded that the ON concentration did not adversely affect growth.

[0051] [Table 2]

[0052] <Sulfate ion concentration and glucosinolate content> The sulfate ion concentration and glucosinolate content are shown in Table 3. Table 3 shows the following: There was no difference in sulfate ion concentration between category 1-1 and categories 1-2 to 1-4. The rate content was lower in categories 1-2 and 1-3 than in category 1-1. In 1-4, the sulfate ion concentration in the nutrient solution was higher than that in 1-1. It was thought that by increasing the concentration to ppm or more, it would be possible to increase the glucosinolate content.

[0053] [Table 3]

[0054] <Test 2: Examination of light intensity and light irradiation time conditions that improve glucosinolate content> <Cultured seedlings> An intergeneric hybrid with kale as the pollen parent and radish as the stigma parent (variety registration application number 33291 The name of the applied variety: Sainte Vert 48) was cultivated and raised in the Kagome Co., Ltd. research laboratory. Cultured seedlings were used for the test. The seedlings used for the test had developed to the fifth leaf (the fifth leaf). The plant height was about 15 cm. The cultured seedlings were planted on February 18, 2021.

[0055] <Cultivation> In categories 2-2 to 2-5, the cultured seedlings were placed in an artificial climate chamber (manufactured by Nippon Medical Equipment Manufacturing Co., Ltd.) for 2 The plants were grown in a 1.5 L container. The basic composition of the nutrient solution was as described in Otsuka et al. A formulation (manufactured by OAT Agrio Co., Ltd.) was used. Commercially available magnesium sulfate was used to reduce the sulfur content of the nutrient solution. The sulfate ion concentration in the nutrient solution was increased to 3000 ppm. The composition of the nutrient solution is shown in Table 4. The solution was continuously aerated using an air pump. The cultivation temperature of the artificial climate chamber was kept constant at 22°C and the humidity at 70%. The light source was an LED (for plant cultivation). A high-intensity LED "Plant Fleck" (Nihon Ika Kikai Seisakusho Co., Ltd.) was used. The irradiation time was as shown in Table 5. Light irradiation as shown in Table 5 was started immediately after planting.

[0056] In category 2-1, the cultured seedlings were grown in a greenhouse owned by Kagome Co., Ltd. (Tochigi Prefecture) in a 13L tank. The plants were grown in a large capacity container and in soil from a planter (manufactured by Cainz Co., Ltd.). The cultivation was carried out in a liquid culture medium, and the nutrient solution used was Otsuka A formulation. The light source was natural light, and the light intensity and illuminance were adjusted. The shooting time was left to chance.

[0057] [Table 4]

[0058] [Table 5]

[0059] <Analysis method> The plants were cultivated for 30 days after planting, and the spread width, sulfate ion concentration, and glucose concentration per plant were measured. The nolate content was measured. In addition, for categories 2-1, 2-2, and 2-5, the The cultivation was continued for 70 days after planting in the same environment, and the spread width per plant, sulphate ion concentration, etc. were measured. The phosphate concentration, glucosinolate content, plant weight, and leaflet yield were measured.

[0060] <Measurement of glucosinolate content> The glucosinolate content was measured by the following method. Two leaves measuring 20 cm in diameter were taken and the blades of these were used. If there were two or more leaves measuring 20 cm in diameter, the blades of the leaves were taken and the blades of the leaves were used. The freshest leaves were used. Three plants were measured in duplicate, and the average value was calculated. Detailed conditions for treatment and HPLC measurement were the same as in Test 1.

[0061] <Measurement of sulfate ion concentration> The sulfate ion concentration was measured by the following method. The measurement site was a 20 cm section from each plant. Two leaves were taken and the blades were used. If there were two or more leaves measuring 20 cm in size, the newer one was used. Leaves were used. Three plants were measured in duplicate, and the average value was calculated. The detailed measurement conditions for the ion chromatography were the same as those in Test 1.

[0062] <Growth Survey> <Opening width> The leaf spread width was measured using a tape measure to measure the width of the plant when viewed from directly above.

[0063] <Stock weight> The roots were removed and the whole plant was weighed.

[0064] <Leaflet yield (weight, number of leaves)> The number and weight of leaflets less than 20 cm in length were measured.

[0065] <Result> <Glucosinolate content> The glucosinolate content is shown in Table 6. From Table 6, it can be seen that: The results were analyzed by Tukey HSD (P<0.05) for the 30-day and 70-day groups after transplantation. Different letters indicate significant differences between groups.

[0066] Thirty days after planting, the glucosinolate content was higher in the plants of Class 2-2 to Class 2-3 than in Class 2-1. The increase was highest in category 2-2, category 2-3, category 2-4, and category 2-5. This shows that the stronger the light intensity and the longer the irradiation time, the higher the It was found that it increased the content of glucosinolates. Furthermore, a comparison of Class 2-3 and Class 2-4 Therefore, light intensity has a stronger effect on glucosinolate content than light exposure time. I found out.

[0067] At 70 days after planting, the glucosinolate content in category 2-1 remained almost unchanged. However, in categories 2-2 and 2-5, the glucosinolate content increased by 30 days. The glucosinolate content was the highest in categories 2-5.

[0068] [Table 6]

[0069] <Relationship between sulfate ion concentration and glucosinolate content> The average sulfate ion concentration in each test plot 70 days after planting is shown in Figure 3. The relationship between ion concentration and glucosinolate content is shown in Figure 4. From a comparison of categories 2-1, 2-2 and 2-5, the sulfate ion concentration in the nutrient solution It was found that increasing the concentration of sulfate ions in the leaves also increased the concentration of sulfate ions in the leaves. A positive correlation was observed between sulfate ion concentration and glucosinolate content, which suggests that the sulfate concentration in the nutrient solution is important. It was believed that the glucosinolate content increased by increasing the acid ion concentration.

[0070] <Growth Survey> The appearance of the plants in each category is shown in Figure 5. The results of measurements of the spread width, plant weight, and leaflet yield (weight and number of leaves) The results are shown in Table 7. From this, the following can be seen. The test was performed using Tukey HSD (P <0.05, N=6).

[0071] Thirty days after planting, no significant difference was observed in the width of the opening among the test plots. No adverse effects on growth were observed due to the shot. On the other hand, 70 days after planting, the plants were classified as 2-1. In all the sections 2-2 to 2-5, the spread width was smaller. The weight of the plant was smaller in categories 2-2 and 2-5 than in category 2-1. This is presumably due to the influence of long-term strong light treatment and long-term irradiation on the growth of the plants. In the case of category 2-5, the weight of the plant was smaller than that of category 2-1, but the group per weight was It has a high glucosinolate content, which means you can get the necessary amount of glucosinolates with a small amount of food. This shows that it is possible.

[0072] Based on the results of leaflet yield and number of leaflets, in category 2-5, many small leaves of 5-15 cm were formed. The spread width and plant weight were affected in the examples, while in categories 2-5, It was found that the same amount of leaflets can be harvested compared to category 2-1. It is softer than the larger leaves and is highly suitable for fresh food. It has a high glucoraphanin content per unit weight and many leaflets that are highly suitable for fresh food. This indicates that harvest is possible.

[0073] [Table 7]

[0074] The results of Test 1 and Test 2 show the following: Increasing the light intensity and lengthening the irradiation time increased the glucosinolate content. Furthermore, this effect was observed for a long period of time, 70 days after planting. It was found that this was maintained even when [Industrial Applicability]

[0075] A field in which the invention is useful is vegetable production.

Claims

1. A method for increasing the glucosinolate content of cruciferous vegetables, comprising at least At a minimum, the process is as follows: Cultivation: Cruciferous vegetables are cultivated here. In the cultivation, the cruciferous vegetables are given a nutrient solution, The sulfate ion concentration of the nutrient solution is 3000 ppm or more, The cultivation period is 30 days or more.

2. 2. The method of claim 1, In the above cultivation, the cruciferous vegetables are irradiated with artificial light.

3. 3. The method of claim 2, The cumulative illuminance of artificial light irradiated to the Brassica vegetables in the above cultivation is 240 0 μmol m -2 ・s -1 ・h or more.

4. 4. The method of claim 3, The light intensity and light irradiation time of the artificial light irradiated to the Brassicaceae vegetables in the cultivation are 400 μmol m -2 ・s -1 or more and 12 hours or more per day, or μmol m -2 ・s -1 That's all, and 24 hours per day.

5. The method of any one of claims 1 to 4, The cultivation period in the above cultivation is 70 days or more.

6. The method of any one of claims 1 to 4, The cruciferous vegetable is an intergeneric hybrid between a vegetable of the genus Brassica and a vegetable of the genus Radish.

7. 6. The method of claim 5, The cruciferous vegetable is an intergeneric hybrid between a vegetable of the genus Brassica and a vegetable of the genus Radish.

8. A method for producing glucosinolate-containing cruciferous vegetables comprising at least In either case, the process is as follows: Cultivation: The vegetables grown here are glucosinolate-containing cruciferous vegetables. In the above cultivation, the glucosinolate-containing cruciferous vegetables are given a nutrient solution. the law of nature, The sulfate ion concentration of the nutrient solution is 3000 ppm or more, The cultivation period is 30 days or more.

9. 9. The method of claim 8, comprising: In the cultivation, the glucosinolate-containing cruciferous vegetables are irradiated with artificial light. do.

10. 10. The method of claim 9, comprising: The integrated illuminance of artificial light irradiated to glucosinolate-containing cruciferous vegetables during cultivation is 2400 μmol m per day -2 ・s -1 ・h or more.

11. 10. The method of claim 9, comprising: The light intensity of the artificial light irradiated to the glucosinolate-containing cruciferous vegetables during the cultivation and the light irradiation time is 400 μmol m -2 ・s -1 or more and 12 hours or more per day, or 100 μmol m -2 ・s -1 That's all, and 24 hours per day.

12. 12. A method of production according to any one of claims 8 to 11, comprising the steps of: The cultivation period in the above cultivation is 70 days or more.

13. 12. A method of production according to any one of claims 8 to 11, comprising the steps of: The glucosinolate-containing cruciferous vegetables are Brassica vegetables and Radish vegetables. It is an intergeneric hybrid.

14. 13. The method of claim 12, further comprising the steps of: The glucosinolate-containing cruciferous vegetables are Brassica vegetables and Radish vegetables. It is an intergeneric hybrid.

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