Composition for scalp and / or hair

Abstract

To provide a composition for promoting hair growth.SOLUTION: Provided is a composition for scalp and / or hair, comprising one or more selected from Hibiscus sabdariffa, Pueraria root, and Capsicum annuum.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to a composition to be applied to the scalp and / or hair. [Background technology]

[0002] New hair that grows from the hair root grows for a certain period of time, then stops growing and falls out, repeating this cycle. To keep hair healthy, it is important to maintain a normal hair cycle.

[0003] It is well known that aging causes the above hair cycle to not function normally, and hair thinning and hair loss progresses, but in recent years, due to the increase in social stress and the disturbance of lifestyle habits, even relatively young people have more and more cases of hair cycle not functioning normally, and hair thinning and hair loss progresses.In addition, in recent years, it has been revealed that not only aging but also obesity promotes hair thinning and hair loss (see Non-Patent Document 1).

[0004] Regarding thinning hair and hair loss, various methods for preventing and treating the condition have been proposed, and the development of effective hair growth agents and the like is underway (for example, Patent Document 1).

[0005] In addition, it is known that various moisturizing agents and oils are blended in hair growth agents to maintain the moisturizing feeling of the scalp and hair. However, there is a concern that some ingredients for maintaining the moisturizing feeling may make the scalp and hair sticky and cause a poor feeling of use. Therefore, development of hair growth agents that maintain a moisturizing feeling and have an excellent feeling of use is being promoted. For example, it has been disclosed that a hair growth agent blended with an extract of loquat leaves exhibits a hair growth effect and is not sticky when used (Patent Document 2). However, this technology is insufficient to exhibit an excellent moisturizing feeling and feeling of use, and further development of hair growth agents is required. [Prior art documents] [Patent documents]

[0006] [Non-Patent Document 1] http: / / www.qlifepro.com / news / 20210625 / obesity-accelerates-hair-thinning.html [Patent Document 1] Patent Publication No. 2021-172596 [Patent Document 2] JP 2010-105932 A Summary of the Invention [Problem to be solved by the invention]

[0007] An object of the present invention is to provide a novel composition that promotes hair growth. [Means for solving the problem]

[0008] The present inventors discovered that hibiscus, pueraria root, and chili pepper have the effect of promoting the expression of COL17A1 (collagen type XVII alpha 1 chain) and activating the SHH (Sonic hedgehog) signal, and found that these effects promote hair growth, thereby completing the present invention.

[0009] That is, the present invention is as follows. [1] A composition for the scalp and / or hair, comprising one or more selected from hibiscus, pueraria lobata, and chili pepper. [2] The composition described in [1], which is a hair growth composition. [3] The composition described in [1] or [2], characterized in that it is a composition for promoting COL17A1 expression and / or activating SHH signaling. [4] The composition described in any one of [1] to [3], which has a moisturizing effect. [5] The composition described in any one of [1] to [4], which is an external preparation. Effect of the Invention

[0010] The composition of the present invention promotes hair growth through the promoting effect of COL17A1 expression and / or the activating effect of SHH signaling. The composition of the present invention promotes hair growth through the promoting effect of COL17A1 expression and / or the activating effect of SHH signaling, and furthermore, can exert an excellent moisturizing feeling. [Brief description of the drawings]

[0011] [Figure 1] FIG. 1 shows the expression level (relative value) of the COL17 gene in the composition of the present invention (hibiscus). [Diagram 2] FIG. 1 shows the expression level (relative value) of the COL17 gene in the composition of the present invention (Kakkon). [Diagram 3] FIG. 1 is a graph showing the expression level (relative value) of the COL17 gene in the composition of the present invention (chili pepper). [Figure 4] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (hibiscus). [Diagram 5] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (Kakkon). [Figure 6] FIG. 1 shows the luciferase activity (SHH signal activity) (relative value) of the composition of the present invention (capsicum). [Figure 7] FIG. 2 is a graph showing the rate of change in stratum corneum moisture content for the compositions of the present invention (hibiscus, pueraria lobata). DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0012] The composition of the present invention is applied to the scalp and / or hair, and is characterized by containing one or more selected from hibiscus, pueraria root, and chili pepper (hereinafter, sometimes referred to as the components of the present invention). It is preferable that the composition of the present invention contains two or more components of the present invention in order to enjoy the effects of the composition more effectively.

[0013] The composition of the present invention promotes hair growth through the promoting effect of COL17A1 expression and / or the activating effect of SHH signaling.

[0014] Hair follicles play an important role in hair growth, and these hair follicles are maintained by the proliferation of hair follicle stem cells, which are the source of hair, and their differentiation into hair matrix cells. COL17A1 acts on the normal cell division (proliferation and differentiation into hair matrix cells) of hair follicle stem cells. Therefore, the composition of the present invention can promote the expression of COL17A1, and thereby promotes normal cell division of hair follicle stem cells and inhibits aging of hair follicle stem cells, thereby exerting a hair growth effect.

[0015] In addition, when COL17A1 is decreased, the division of hair follicle stem cells is altered, and the hair follicle stem cells differentiate into epidermal cells, resulting in a decrease in the number of hair follicle stem cells. In aged hair follicle stem cells, this COL17A1 is decreased. Therefore, the composition of the present invention can be said to exhibit a hair growth effect by promoting the expression of COL17A1, inhibiting the decrease in the number of hair follicle stem cells, and inhibiting aging.

[0016] In addition, when SHH signaling is suppressed, hair follicle stem cells differentiate into epidermal cells and sebaceous glands, resulting in a decrease in the number of hair follicle stem cells. The composition of the present invention can activate SHH signaling, thereby suppressing the decrease in the number of hair follicle stem cells and exerting a hair growth effect.

[0017] As mentioned above, obesity is a cause of thinning hair and hair loss (see Non-Patent Document 1), and since obesity suppresses the SHH signal, the composition of the present invention can activate the SHH signal and promote hair growth in thinning hair and hair loss caused by obesity.

[0018] Therefore, the composition of the present invention can be used as a composition for hair growth, a composition for promoting COL17A1 expression, a composition for activating SHH signal, etc. The concept of hair growth in the present invention includes hair growth and hair care.

[0019] [hibiscus] Hibiscus is a plant of the genus Hibiscus in the family Malvaceae, and its scientific name is Hibiscus sabdariffa, and it is also called roselle. In the present invention, various parts such as flowers, leaves, bark, roots, seeds, etc. can be used, but it is preferable to use flowers because they can more effectively promote the expression of COL17A1 and / or activate the SHH signal, and can also more effectively promote the expression of COL17A1 and / or activate the SHH signal, and can also exhibit excellent moisturizing effects. The flowers may include calyxes, etc., in addition to petals.

[0020] Hibiscus can be used, for example, as a crushed product, a squeezed juice, an extract, etc. Examples of crushed products include a dried powder and a finely cut product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred because it can better enjoy the effects of the present invention and can be easily blended into a composition. The temperature of each solvent can be appropriately set between room temperature and the boiling point. In the present invention, an extract of water, BG, or aqueous BG is preferred because it can better enjoy the effects of the present invention.

[0021] [Cuckoo] Kudzu is a creeping perennial plant of the Pueraria genus of the legume family, and Kakkon is the root of Kudzu. The type of Kudzu that can be used in the present invention is not particularly limited, but can be exemplified by Pueraria thomsonii, Pueraria lobata, Pueraria thunbergiana, etc., and Pueraria lobata is preferred from the viewpoint of availability and various effects.

[0022] Pueraria lobata can be used, for example, as a pulverized product, a squeezed juice, an extract, etc. Examples of pulverized products include a dried powder and a finely shredded product. The squeezed juice and extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred in terms of the fact that the effects of the present invention can be more effectively enjoyed and the ease of incorporation into the composition. The extract can be obtained by extraction using a suitable solvent, and the solvent is not particularly limited. However, hydrophilic solvents such as water, ethanol, aqueous ethanol, 1,3-butylene glycol (BG), and aqueous BG are preferred in terms of the fact that the effects of the present invention can be more effectively enjoyed and the ease of incorporation into the composition. The temperature of each solvent can be appropriately set within the range of room temperature to the boiling point or lower. In the present invention, an extract of water, BG, or aqueous BG is preferred in terms of the fact that the effects of the present invention can be more effectively enjoyed.

[0023] [Chili pepper] The chili pepper is not particularly limited as long as it contains capsaicin or a capsaicinoid-like substance, and the variety, the place of origin, etc. are not particularly limited. Specific examples include Fushimi Kancho, chili pepper variety CH-19 Sweet, Shishito, Yamashina, Manganji, Eagle Talon, Kagawa Hontaka, Aomori Eagle Talon, Sapporo Daicho, California Wonder, Cherry Bomb, etc. Various types of chili peppers may be used alone or in combination of two or more types. The part used in the present invention is preferably a part containing capsaicin or a capsaicinoid-like substance, and since capsaicin or a capsaicinoid-like substance is contained in large amounts in the placenta, it is preferable to use the placenta or a fruit containing the placenta.

[0024] Capsicum can be used, for example, as a pulverized product, a squeezed juice, an extract, etc. Examples of pulverized products include a dried powder and a finely shredded product. The squeezed juice or extract may be in a liquid form, but can also be used in a paste form or a dried powder (juice powder, extract powder). In the present invention, an extract is preferred because it can better enjoy the effects of the present invention and can be easily incorporated into the composition. The extract can be obtained by extraction using a suitable solvent, and examples of the solvent that can be used include water, ethanol, and aqueous ethanol. The temperature of each solvent can be appropriately set within the range of room temperature to the boiling point or lower. In the present invention, an extract of ethanol or aqueous ethanol is preferred because it can better enjoy the effects of the present invention.

[0025] The composition of the present invention can be produced by a known method by adding other components, if necessary, in addition to the components of the present invention. Examples of other components include water, astringents, bactericides, antibacterial agents, ultraviolet absorbers, moisturizers, cell activators, anti-inflammatory agents, antioxidants, active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, fragrances, and plant extracts other than hibiscus, pueraria root, and capsicum.

[0026] The composition of the present invention can be used as an external preparation such as a medicine (including quasi-drugs) or a cosmetic. The external preparation is not particularly limited as long as it can be applied to the scalp and / or hair, and examples of the form include liquid, ointment, cream, gel, etc. Specific examples include shampoo, conditioner, rinse, treatment (including leave-in type), hair lotion, hair emulsion (hair cream), hair gel, hair cream, hair oil, hair pack, hair mask, hair tonic, hair liquid, hair spray, hair styling product, hair growth product, hair growth product, hair care product, etc.

[0027] The composition of the present invention can be used as a hair growth composition, and the hair growth composition is not particularly limited as long as it contains at least one of the components of the present invention and can be distinguished from other products in terms of being used for hair growth, and for example, the scope of the present invention includes products that display functions related to hair growth on the main body, packaging, instructions, or promotional materials (advertising media) of the product of the present invention. Specifically, examples of such products include, in addition to displays such as hair growth products (hair growth agents), hair growth products (hair growth agents), and hair care products (hair care agents), displays that include phrases such as "give hair firmness and strength," "for those who want firmness and strength," "improve scalp care effects," "care for scalp and hair," "prevent hair loss," "for those who are concerned about thinning hair," and "for healthy hair."

[0028] The composition of the present invention can be used as a composition for promoting COL17A1 expression, and the composition for promoting COL17A1 expression is not particularly limited as long as it contains at least one of the components of the present invention and can be distinguished from other products as a product in that it is used to promote COL17A1 expression, and for example, the scope of the present invention includes a product that displays a function related to promoting COL17A1 expression on the main body, packaging, instructions, or promotional material (advertising medium) of the product of the present invention. Specifically, examples of the display include phrases such as "promotes COL17A1 expression" and "prevents aging of hair follicle stem cells."

[0029] The composition of the present invention can be used as a composition for activating SHH signaling, and such a composition for activating SHH signaling is not particularly limited as long as it contains at least one of the components of the present invention and can be distinguished from other products in that it is used to activate SHH signaling, and for example, the scope of the present invention includes products that display a function related to SHH signaling activation on the main body, packaging, instructions, or promotional materials (advertising media) of the product of the present invention. Specifically, for example, display including the phrase "activates SHH signaling" can be exemplified.

[0030] The hair growth composition of the present invention may be one in which the ingredient of the present invention is indicated as an active ingredient, but is not limited to one in which the ingredient of the present invention is indicated as an active ingredient. For example, it may be one in which the active ingredient is not indicated. In addition, those manufactured and sold with the function and use suggested are included in the scope of the present invention. For example, there is a product sold with the experience testimonials of the person who took it, which mention the function and use as personal impressions, posted on the homepage, etc.

[0031] The content of the component of the present invention in the composition of the present invention (total amount when two or more kinds are used in combination) may be appropriately determined within the range in which the effect is exhibited. For example, the component of the present invention may be contained in the composition of the present invention in an amount of 0.0001 to 50 mass%, preferably 0.001 to 20 mass%, more preferably 0.01 to 10 mass%, and even more preferably 0.1 to 5 mass%, calculated as a dry mass. EXAMPLES

[0032] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

[0033] [Example 1] In order to confirm the hair growth effect of the composition of the present invention, the COL17A1 expression promoting effect of the components of the present invention was confirmed.

[0034] <Test substance> As for the hibiscus, a hibiscus flower extract obtained by extracting hibiscus flowers with aqueous BG was used. For the Pueraria lobata extract, we used pueraria lobata root extract, which was extracted with aqueous BG. As the chili pepper, a chili pepper fruit extract obtained by extracting chili pepper fruit with aqueous ethanol was used.

[0035] <Test Method> The test was conducted according to the following steps (1) to (9). (1) In a 37℃, 5% CO2 incubator, 75 cm 2A human epidermal keratinocyte cell line was cultured in a flask using 10% FBS-DMEM medium.

[0036] (2) The cells were suspended by trypsinization and then transferred to a 75 cm 2 From the flask, transfer 8 x 10 cells to a collagen-coated 96-well plate. 3 100 μL of cells were seeded to give a cell density of 10 cells / well.

[0037] (3) The cells were pre-cultured overnight in a 37°C, 5% CO2 incubator, and after removing the culture supernatant, the cells were washed once with 150 μL of DMEM. DMEM was then added and the cells were cultured for 2 hours.

[0038] (4) After removing the medium from each well, 100 μL / well of medium containing the test substance was added.

[0039] (5) The medium containing the test substance was prepared as follows. The test substance was dissolved in 10% FBS-DMEM to a concentration of 10% (v / v) to prepare a stock solution. The prepared stock solution was further diluted with 10% FBS-DMEM to adjust the concentration to 1%, and after filter sterilization, further dilution was repeated. Media containing 1%, 0.2%, and 0.04% of the test substance were used as samples for addition. In addition, only 10% FBS-DMEM without the test substance was added as a control.

[0040] (6) After adding the medium containing the test substance, the cells were cultured in a 37°C, 5% CO2 incubator for 24 hours, and then the culture supernatant was removed. Each well was washed once with 150 μL of PBS and incubated with RNeasy (R) RNA was extracted using a Mini Kit (QIAGEN).

[0041] (7) From the extracted RNA, ReverTra Ace (R) cDNA was synthesized using qPCR RT Master Mix with gDNA Remover (Toyobo).

[0042] (8)QuantiNova (R)SYBR (R) The expression level of the COL17 gene as the target gene was evaluated by real-time PCR using the Green PCR Kit (QIAGEN). The internal standard was RRN18S, and primers from QIAGEN were used.

[0043] (9) The relative gene expression level was calculated by setting the control gene expression level as 1.

[0044] The results are shown in Figures 1 to 3. 1 to 3, hibiscus, pueraria root, and capsicum showed significant promoting effects on COL17A1 expression. Therefore, the composition of the present invention has a promoting effect on COL17A1 expression and promotes hair growth.

[0045] [Example 2] In order to confirm the hair growth effect of the composition of the present invention, the SHH signal activation effect of the components of the present invention was confirmed.

[0046] <Test substance> The hibiscus, pueraria lobata and chili pepper used were the same as those used in Example 1.

[0047] <Test Method> The test was conducted according to the following steps (1) to (5). (1) In a 37℃, 5% CO2 incubator, 75 cm 2 GliReporter-NIH3T3 cells (Funakoshi) were cultured in flasks using 10% CS-500 μg / mL Geneticine-DMEM medium.

[0048] (2) The cells were suspended by trypsinization and then transferred to a 75 cm 2 From flask to 96-well plate: 2.5 x 10 4 100 μL of cells were seeded to a cell density of 10 cells / well. 10% CS-DMEM was used as the medium.

[0049] (3) After overnight pre-incubation in a 5% CO2 incubator at 37°C, the medium was removed, and 50 μL of the prepared test substance solution serially diluted with the medium was added. As a control, medium without the test substance was also added. The medium used was 0.5% CS-1% NEAA-1 mM Na pyruvate-10 mM HEPES-OptiMEM. The test substance solution was filter sterilized before use.

[0050] (4) After culturing for 24 hours in a 37°C, 5% CO2 incubator, luciferase activity (SHH signal activity) was evaluated using a Luciferase Assay Kit (Applied Biological Materials).

[0051] (5) The relative luciferase activity value was calculated by setting the control luciferase activity value as 1.

[0052] The results are shown in Figures 4 to 6. As shown in Figures 4 to 6, hibiscus, pueraria root and capsicum all promoted luciferase activity (SHH signal activity). Therefore, the composition of the present invention has an SHH signal activating effect and can promote hair growth.

[0053] [Example 3] In order to confirm the moisturizing effect of the composition of the present invention, the effect of the components of the present invention in increasing the moisture content of the stratum corneum was confirmed.

[0054] <Test substance> The hibiscus and pueraria lobata used were the same as those used in Example 1. In addition, as the carrot extract, a ginseng root extract obtained by extracting ginseng root with aqueous ethanol was used.

[0055] <Test Method> (1) The insides of the forearms of three subjects were washed before application of the test substance.

[0056] (2) After leaving it to stand for 15 minutes, the moisture content of the stratum corneum at the planned application sites of the test substance on the inside of the forearms of the three subjects (each 2 cm long and 2 cm wide) was measured using the measuring device described below.

[0057] (3) 0.02 mL of the test substance was applied to each of the areas on the inside of the forearms of the three subjects where the test substance was to be applied, and after leaving it for 15 minutes, the moisture content of the stratum corneum at the areas where the test substance was applied was measured in the same manner.

[0058] (4) The average value of the test substance application site after use of the skin topical agent was divided by the average value before use, and the resulting value was taken as the rate of change. The rate of change in stratum corneum moisture content is shown in Figure 9. In these figures, the higher the rate of change, the higher the evaluation.

[0059] The stratum corneum moisture content was measured using a Corneometer CM825 (Courage+Khazaka). This stratum corneum moisture content measurement device evaluates the moisture content of the stratum corneum as skin conductance (electrical conductivity, unit: μS). Measurements were performed using the standard method described in the instruction manual that came with the device.

[0060] As shown in Figure 7, both hibiscus and pueraria root increased the moisture content of the stratum corneum. Therefore, the composition of the present invention can increase the moisture content of the stratum corneum and exert a moisturizing effect.

[0061] [Composition Examples 1-1 to 1-5] (Manufacturing of hair growth products) A hair growth agent was produced comprising the components shown in Table 1 below. This hair growth agent exhibits the effects of the present invention.

[0062] [Table 1]

[0063] [Composition Examples 2-1 to 2-5] (Shampoo manufacturing) A shampoo was prepared from the components shown in Table 2 below. This shampoo exhibits the effects of the present invention.

[0064] [Table 2]

[0065] [Composition Examples 3-1 to 3-5] (Treatment manufacturing) A treatment was produced from each component listed in Table 3 below. This treatment exhibits the effects of the present invention.

[0066] [Table 3]

[0067] [Composition Examples 4-1 to 4-5] (Hair lotion manufacturing) A lotion was produced from each component shown in Table 4 below. This hair lotion exhibits the effects of the present invention.

[0068] [Table 4]

[0069] [Composition Examples 5-1 to 5-5] (Manufacture of hair lotion (hair milk)) A milky lotion was produced from each of the components shown in Table 5 below. This hair milky lotion exhibits the effects of the present invention.

[0070] [Table 5]

[0071] [Composition Examples 6-1 to 6-5] (Hair gel manufacturing) A gel was prepared from the ingredients listed in Table 6 below. This hair gel exhibits the effects of the present invention.

[0072] [Table 6]

[0073] [Composition Examples 7-1 to 7-5] (Hair cream manufacturing) A cream was prepared from the ingredients listed in Table 7 below. This hair cream exhibits the effects of the present invention.

[0074] [Table 7] [Industrial Applicability]

[0075] The composition of the present invention can be used as a hair growth agent and is therefore industrially useful.

Claims

1. A composition for the scalp and / or hair, comprising one or more selected from hibiscus, pueraria root and chili pepper.

2. 2. The composition according to claim 1, which is a hair growth composition.

3. The composition according to claim 1, which is a composition for promoting COL17A1 expression and / or activating SHH signaling.

4. The composition according to any one of claims 1 to 3, which is an external preparation.

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