Time-delayed mite trap and integrated pest management method using the same

Abstract

To provide beekeeping technique to collect pure honey with minimal impurities and chemical contamination through effective integrated eradication and control of varroa mites in the beekeeping industry by a simple method avoiding complexity without requiring labor and without requiring cost / power such as installation of a mechanical device and minimizing use of chemical substances such as pesticides.SOLUTION: A time-delayed mite trap control method uses a honeycomb board, a honeycomb frame, or a honeycomb foundation frame whose depth dimension is two-thirds to one-third of the depth dimension of the inside of a honeybee rearing box, as a mite trap. A time-delayed mite trap is characterized in that honeybees are reared in order of a step 1 to a step 5 so as to be able to eradicate varroa mites. There is also provided an integrated pest control method using the time-delayed mite trap.SELECTED DRAWING: Figure 3

Description

[Technical field]

[0001] The present invention is a technical invention in the beekeeping industry, which raises honeybees and harvests honey, and in particular relates to a method for exterminating Varroa mites, which parasitize honeybees, and a beehive structure for extermination. [Background technology]

[0002] In the beekeeping industry, bees are raised in artificial nests (breeding boxes) such as hives, and honey produced in the artificial nests is collected. These hives have an internal space that is closed except for the hive entrance, which is the entrance and exit for the bees, and multiple comb boards (8 to 10 pieces, etc.), frames and foundations, frames with foundations (combine foundation frames), etc. are arranged in a row, and the bees form cells, which are their nests, in these comb boards and frames. Of course, there are also hives that have 10 to 20 or more comb boards, frames and foundations, and frames with foundations (combine foundation frames) arranged in a row.

[0003] The details of the invention will be explained using drawings etc. below, but in explaining the present invention, the names of special structures within beehives in beekeeping and special names related to honeybees will be described, and similarly, only the outline of necessary information regarding the habits of honeybees and the habits of mites in beekeeping will be explained.

[0004] A comb foundation is a thin plate made of beeswax (officially called "beeswax", but in this application it will be referred to as the common name "beeswax") with a honeycomb structure imprinted on it. Some are made of plastic. Using comb foundations allows bees to build cells more quickly. There are small hexagonal comb foundations for female bees and large hexagonal foundations for male bees. The honeycomb boards and frames are the wooden frames that the honeycomb foundation is attached to and the base boards of the hive. The frame with the honeycomb foundation attached is called the honeycomb foundation. (After the honeycomb cells (beehives) are created, the entire honeycomb frame including the honeycomb cells may be called the honeycomb board.) A honeycomb-shaped cell is a cavity for the bee larvae. It also stores honey and pollen. The cells for male bees are larger than those for worker bees, and the cells for queen bees are even larger and are called queen cells.

[0005] Western honeybees (Apis mellifera) are the most commonly used species for beekeeping in Japan and around the world. Western honeybees used in beekeeping have the advantage of being able to collect large amounts of honey and making it easy to maintain large populations. Japanese honeybees (Apis mellifera) are rarely used in beekeeping because they produce less honey than Western honeybees and are prone to swarming (swarming is the habit of the queen bee and about half of the worker bees leaving the hive they have been using to search for a new nest site) and escaping, but many people prefer the taste and quality of honey produced by Japanese honeybees, so Japanese honeybee beekeeping is also carried out on a small scale.

[0006] In this application, the description will be mainly focused on Western honeybees, but it should be clearly stated that the invention also applies to Japanese honeybees.

[0007] Generally, honeybees form a colony with one queen bee and many worker bees. The queen bee continues laying eggs in the hive, which mainly become worker bees. Worker bees are divided into nursing bees, who secrete beeswax to build honeycomb boards (honeycombs with honeycomb cells), and specialize in maintaining the hive and caring for the larvae, and honeycollection bees (honeygathering bees), who specialize in collecting pollen and nectar, processing it into honey, and storing it. In this application, honeycollection bees are also referred to as worker bees and are distinguished from nursing bees. In addition, nursing bees build large honeycomb cells called queen cells in the honeycomb boards, feed royal jelly to the larvae, and also raise the next generation of queen bees.

[0008] Beeswax is wax excreted from the secretory glands of worker bees and is used to make honeycombs (cells) and as honey caps and cell lids.

[0009] One of the most dangerous mites that harms honeybees is Varroa destructor, and as this application is primarily concerned with Varroa destructor, it will be described below, but it is expressly stated that this application does not exclude other mite species that have similar behavior to that of Varroa destructor used in this application.

[0010] When honeybees are infested with Varroa mites, the fat bodies in the abdomen of the bee, which are important for the insect, are eaten away, causing malnutrition, impaired energy metabolism, and weakened immunity, resulting in fatal effects.

[0011] Furthermore, the impact of mites that parasitize honeybees is largely due to viral infections transmitted by the mites. Nearly 20 types of viral diseases transmitted by mites are known. In particular, five types of viruses have been proven to be transmitted by the Varroa mite: Cashmere virus, Sacbrood virus, Acute paralysis virus, Israeli acute paralysis virus, and Wing deformed virus.

[0012] Western honeybees are susceptible to mites that transmit these viruses, particularly Varroa destructor mites, which parasitize male bees in particular. This is because male Western honeybees have a long pupation period, which ensures that the mites that infiltrate the comb have time to breed and grow. Japanese honeybees are relatively less affected by Varroa destructor mites because their pupation period is short. Western honeybees are currently the mainstream in the beekeeping industry in Japan, and the damage caused by infestations by Varroa destructor mites is enormous. It has also been reported that there has been an increase in highly virulent viruses in recent years.

[0013] Although there are several types of Varroa mites that have been academically classified, in this application, all of them will be referred to as Varroa mites. In the following description of this application, Varroa mites will also be referred to simply as mites.

[0014] Currently, three types of acaricides are registered in Japan. Apistan has been on the market for 27 years, and it is said that mites have already shown strong resistance to it. Apivar is said to be less effective in hot and humid regions such as Japan, and mite resistance is also said to be increasing. Timovar is also said to have similar drawbacks. In addition, some types of acaricides can reduce the quality of the beeswax collected from honeybees. Due to these problems, the beekeeping industry is currently unable to come up with effective countermeasures against mite infestation and reproduction. In recent years, there have been an increasing number of cases where honeybees that beekeepers have worked so hard to raise have disappeared due to mite infection.

[0015] In beekeeping, the damage caused by mites is so great that various techniques have been developed to combat this problem. Pesticides have also been used, but as mentioned above, pesticides are by no means an all-purpose solution. Beekeepers are eager to develop techniques or methods that can completely eradicate mites by reducing the use of pesticides, which cause resistance in mites and a decline in beeswax quality.

[0016] In response to such demands, various proposals have been made in the prior art, such as those listed below.

[0017] For example, Patent Document 1 (JP 2021-40535 A) discloses the following technology.

[0018] In other words, the technical disclosure relates to a pest control device that includes a housing in which a first opening and a second opening are formed, an intake section that is detachably attached to the housing and forcibly takes in outside air into the housing through the first opening, a retention section that is provided within the housing and communicates with the first opening and temporarily retains the outside air taken in by the intake section, and a pest control component generation section that is provided within the housing and communicates with the retention section and the second opening, and generates a pest control component.

[0019] However, the pest control device in Patent Document 1 is a mechanical device that forcibly takes in outside air and temporarily retains it inside, and requires a complex device system and the power (electricity) required to operate it. In addition, a pest control component generating unit is required inside, which also requires power. In other words, it is an expensive and precise mechanical device, and in the honeybee beekeeping industry, where hundreds of beehives (breeding boxes) are handled in some cases, it is not realistic in terms of the cost and operability of the above device.

[0020] In other words, the technical disclosure of the above-mentioned Document 1 does not go far enough to solve the problem of the present invention, which aims to develop an effective mite extermination method that is not complicated, simple, and inexpensive.

[0021] Furthermore, Patent Document 2 (JP Patent Publication No. 2006-124377) discloses a technical disclosure regarding a parasite control composition for honeybee colonies, which contains fenpyroximate as an active ingredient, and a method for using the control composition, for example by contacting honeybees with the active ingredient.

[0022] The above Patent Document 2 discloses a parasite control composition, i.e., a pesticide, and has a different technical concept from the present invention, which aims to effectively control mites in beekeeping by minimizing the amount of the acaricide used. In beekeeping, there is great value in collecting and providing honey that contains as few impurities and chemicals as possible, such as pesticides, and the above Patent Document 2, which assumes the use of a pesticide from the beginning to control mites, is incompatible with this. In addition, as already described, pesticides have the major disadvantage of causing mites to develop resistance, and creating mites resistant to the pesticide over many years of use.

[0023] Utility Model Document 3 (Utility Model Registration No. 3196081) relates to a honeybee parasitic mite extermination device, and in particular to a honeybee parasitic mite extermination device that contains a mite extermination component such as an acaricide or an anti-mite agent. It discloses the technology of a honeybee parasitic mite extermination device that is characterized by having a sheet member that contains one or more materials selected from the group consisting of a paper sheet, a woven fabric sheet, and a nonwoven fabric sheet that contains a mite extermination component, and a mesh member that covers the surface of the sheet member.

[0024] However, the above content of Patent Document 3 also discloses a parasite control composition, i.e., a pesticide, and the technical idea is to impregnate a sheet of paper, nonwoven fabric, etc. with the composition and bring it into contact with honeybee parasitic mites to exterminate them. As with Patent Document 2, the technical idea is different from that of the present invention, which aims to effectively control mites by minimizing the amount of acaricide used. To repeat, in beekeeping, there is great value in collecting and providing pure honey that contains as few impurities and pesticides as possible, and the above Patent Documents 2 and 3, which are premised on the use of pesticides from the beginning to exterminate mites, are ideologically incompatible. We would like to emphasize that the excessive use of pesticides is contrary to the great risk of causing mites to develop resistance and creating mites resistant to pesticides.

[0025] As explained in the above documents 1, 2 and 3, none of them come close to solving the object and problem of the present invention, and they do not even address the problem of the present application.

[0026] The objective of the present invention is to develop a method and beekeeping system for the beekeeping industry that effectively and comprehensively eradicates and controls Varroa mites in the simplest possible way, without requiring much labor from beekeepers or incurring costs for large equipment, and that minimizes the use of chemical substances such as pesticides, and that allows the collection of pure honey with minimal impurities and chemical contamination. [Prior art documents] [Patent documents]

[0027] [Patent Document 1] Patent Publication No. 2021-40535 [Patent Document 2] JP 2006-124377 A [Patent Document 3] Utility Model Registration No. 3196081 Summary of the Invention [Problem to be solved by the invention]

[0028] The present invention is intended to solve the problems and issues of the conventional technology as described above. In other words, the objective of the present invention is to develop a beekeeping technique in the beekeeping industry that effectively performs comprehensive extermination and control of Varroa mites in a manner that is not complicated and is as simple as possible, does not require the beekeeper's labor, does not require the installation of mechanical devices, and minimizes the use of chemical substances such as pesticides, and allows the collection of pure honey with minimal contamination of impurities and chemical substances, and to develop and provide the method and beekeeping system for this purpose. In addition, a method for solving the above problems that arises from the concept of Integrated Pest Management (IPM) for minimizing the use of pest control chemicals, which is the basic concept of the present invention, is also described as one aspect of the present invention (described in claim 8). Furthermore, what is important about the present invention is that it has been devised as an invention for solving a problem by making maximum use of the natural ecology and habits of honeybees and Varroa mites.

[0029] Here, we will describe only the necessary information about the habits of Varroa mites to be exterminated and controlled. (For details, please refer to a biology book.)

[0030] Varroa mites (hereinafter, mites including Varroa mites are collectively referred to as "mites") parasitize both male and female honeybees, but the smell (pheromone) of male honeybee larvae seems to be very attractive to mites that are about to lay eggs, so they will willingly sneak into a male larvae cell if one is present. They enter just before the male honeybee larvae's cell is covered (the period just before the larvae pupate), and it is thought that they do so in order to recognize and identify the change in the chemical substance (pheromone) emitted from the body surface of the male honeybee larvae that are about to pupate (about to pupate). These chemical substances are said to be odorous substances that are mainly composed of fatty acid esters, carboxylic acids, and hydrocarbons.

[0031] The female mites that entered the male honeybee larvae's cell lay eggs, hatch, and become adult mites during the 14 to 15 days between the time the male larvae emerge from the cell and the time the cell is covered to pupate. There are 5 to 6 of them in one cell, and when the male honeybee emerges from the cell, they leave and spread to other bees (worker bees) by parasitizing them. Considering the number of male honeybee cells, an enormous number of mites will be generated.

[0032] Next, the behavior of honeybees will be briefly described, based on only what is necessary for the present invention. (For details, please refer to any biology book.) Honeybees are classified into male bees, female bees (nursing bees who are worker bees, and honey-gathering bees who are worker bees), and queen bees, and each has a different length of time from when the eggs hatch to the larval stage, pupal stage, and emergence as an adult. The period from the male bee larvae's capping of the cells to their pupation and emergence is about 14 to 15 days, with the pupal period lasting about 13 days. The period from when the female bee larvae cover the cells to when they pupate and emerge is about 12 to 13 days, with the pupal period lasting about 10 days. Incidentally, the period from when the queen bee larvae are lidded to when they pupate and emerge is about 6 to 7 days, with the inter-pupal period being about 6 days.

[0033] As mentioned above, female mites that enter the honeybee larvae's cell lay one egg every day from about three days after entering, from the time the cell is covered until the honeybee larvae pupate and emerge, and the eggs become adult mites about six days later. About six adult mites grow from one female mite in the male honeybee cell, which has the longest pupal period, and when the honeybee pupa emerges, the mites also emerge from the cell to parasitize other honeybees. After the honeybee emerges, the unhatched mite eggs in the cell die.

[0034] In other words, considering the difference in the period from when male and female honeybee larvae cover the cells to when they emerge, it is most convenient for mites to reproduce by entering and laying eggs in cells of male bee larvae, which have a longer pupal period. Therefore, it is thought that in nature, mites have developed the habit of detecting the chemical substances released when male bees pupate, and preferentially entering cells where the male bees pupate.

[0035] The important point of this invention is that it has devised a means of solving the problem in accordance with natural ecology, which utilizes the ecology and habits of honeybees and mites to the maximum extent possible to exterminate and control mites.In addition, there are many other aspects that utilize the habits of honeybees and mites to the maximum extent possible in the same way, so these will be described and explained as they arise. [Means for solving the problem]

[0036] In order to solve such problems, the time-delay mite trap and the comprehensive pest management method using the same according to the present invention are composed of two inventions, namely, an invention of comb boards and / or frames and / or hive foundation frames that are installed in beehive rearing boxes to serve as a mite trap, and an invention of a comprehensive pest management method for mites that uses the comb boards and / or frames and / or hive foundation frames as a time-delay mite trap.

[0037] (Claim 1) The time-difference mite trap and integrated pest management method using the same according to the present invention as described in claim 1 are inventions of comb boards and / or frames and / or comb foundation frames inserted into a beekeeping box, the depth dimension of the comb boards and / or frames and / or comb foundation frames being two-thirds to one-third of the depth dimension inside the beekeeping box, and the comb foundation frames being comb foundations for female bees, characterized in that the lower part of the comb boards and / or frames and / or comb foundation frames makes it easy for worker bees to create male honeycomb cells.

[0038] The comb boards, frames and base frames of beekeeping boxes used in beekeeping will be explained below with reference to FIG.

[0039] The comb boards, frames and foundation frames used in a normal beekeeping box (hereinafter also referred to as a beehive box) are made according to the inside dimensions of the box, with the depth dimension being made to match the depth dimension inside the box and the width dimension being made to match the width dimension inside the box. Usually, the width dimensions of the comb boards, frames and foundation frames are arranged in the box so that they are preferably sized (comb spacing of about 8mm to 9mm) in consideration of the required space width from the viewpoints of maintaining a bee density that allows bees to be active easily, the thickness of the honeycomb to be made and the overall efficient density inside the box. A standard beekeeping box is structured so that 10 comb boards, frames or foundation frames can be placed within the inside length dimension, but the number of combs arranged may be more than 10 or less than 10 as long as the required spacing between them is maintained as described above. Therefore, what is important is that the depth dimensions of the conventional comb boards, frames and foundation frames used in a beekeeping box are approximately the same as the inside depth dimensions of the box.

[0040] However, the depth dimension of the comb board, frame or base frame used in the time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 1 is two-thirds to one-third of the depth dimension inside the honeybee rearing box, and is characterized by providing a space below it. This makes it easier for worker bees to habitually create slightly larger male honeycomb cells, which is an important technical feature.

[0041] In order to increase the number of bees in a breeding box (to increase the honeybee colony), worker bees have a habit of energetically building cells for raising worker bee (female bee) larvae, and when a certain number of worker bee cells are secured in other comb boards or frames, they have a habit of building slightly larger cells for raising male bee larvae for the purpose of mating with a queen bee. Therefore, the comb boards, frames or foundation frames used in the time-difference mite trap and the integrated pest management method using the same according to the present invention have dimensions of 2 / 3 to 1 / 3 of the hive depth, and have a special structure according to the present invention intended to have sufficient worker bee (female bee) cells built in advance in that part, and then have the cells for raising male bee larvae as a mite trap built in the space part of 1 / 3 to 2 / 3 of the hive depth dimension at the bottom.

[0042] In other words, when a beehive is used for the beehive board, beehive frame, or beehive foundation frame of the time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 1, it is preferable to use a beehive foundation for worker bees (female bees). Similarly, it is preferable to set up in advance beehive foundations for worker bees (female bees) for all other beehive boards, beehive frames, and beehive foundation frames that are not used for the time-difference mite trap in the same breeding box, because this sets up a state in which there are insufficient male honeycomb cells. This is because when a beehive board, beehive frame, or beehive foundation frame used for the time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 1 is inserted, the bees are more likely to actively create only male honeycomb cells that become mite traps in the space below.

[0043] As described above, in other comb boards, frames or foundation frames that are not used in the time-difference mite trap of the present invention and the integrated pest management method using it, worker bees actively create large numbers of worker bee (female bee) cells, so the lower space of the comb boards, frames or foundation frames used in the time-difference mite trap of the present invention and the integrated pest management method using it cleverly utilizes the habits of honeybees that make it even easier for male honeycomb cells to be created.

[0044] In the male honeycomb chambers made in the honeycomb boards, frames or foundation frames used in the time-difference mite trap and the integrated pest management method using the same according to the present invention, the queen bee lays male bee eggs, and the hatched larvae grow from the first to fifth instars and become pupae, so the queen bee emits a substance (scented components of fatty acid esters, carboxylic acids, and hydrocarbons) that mainly consists of fatty acid esters and instructs the worker bees to cover the chambers. This substance is a mite attractant, and mites, especially Varroa destructor mites, are attracted and enter the male honeycomb chambers in large numbers, after which the chambers are covered. Of course, during the male honeycomb period after the chambers are covered, the male pupae are parasitized by mites, and the mites actively lay eggs, hatch, and reproduce in the chambers.

[0045] In other words, as described above, the time-difference mite trap of the present invention and the integrated pest management method using it have a mechanism in which male bee larvae emit a mite attractant in male honeycomb cells concentrated in a specific part of the hive using comb boards, frames or base frames, attracting large numbers of mites and trapping the mites inside the male honeycomb cells.This is the mechanism behind the time-difference mite trap of the present invention and the mite trap invention of the integrated pest management method using it.

[0046] (Claim 2) The time-difference mite trap and integrated pest management method using the same according to the present invention as described in claim 2 are inventions of a time-difference mite trap and a comb frame and a comb foundation frame used in an integrated pest management method using the same, characterized in that the comb boards and frames and a comb foundation frame used in the integrated pest management method using the time-difference mite trap are comb boards and / or frames and / or comb foundation frames inserted into a beekeeping box, the depth dimension of the comb boards and / or frames and / or comb foundation frames being two-thirds to one-third of the depth dimension inside the beekeeping box, and the comb foundation frames are comb foundations for female bees, and further, one or more comb frames and / or male beehive foundations made of beeswax and / or male beehive foundation frames made of beeswax of any size are connected to the bottom of the comb boards and / or frames and / or comb foundation frames to make it easier to create male honeycomb cells.

[0047] That is, the honeycomb boards, frames and beehive foundation frames used in the integrated pest management method using the time-difference mite trap described in claim 2 are provided with one or more honeycomb frames or beeswax male honeycomb foundation frames of any size in the space below the honeycomb boards, frames and beehive foundation frames described in claim 1. For example, as shown in parts b and c in FIG. 2 described later, honeycomb frames or beeswax male honeycomb foundation frames are additionally installed in succession. There is no limit to the size of the surface of the connected honeycomb frames or beeswax male honeycomb foundation frames or beeswax male honeycomb foundation frames, and there is no limit to the number. It goes without saying that they must be of a size that can fit inside the hive. In addition, because the connected honeycomb frames or beeswax male honeycomb foundation frames or beeswax male honeycomb foundation frames are small, the part that is not connected to the lower part may remain empty.

[0048] Honeybees will actively create male cells in the additional frames, beeswax male comb foundations, or comb foundation frames that are installed in succession. When there are beeswax male comb foundations, honeybees will actively create male cells earlier. Also, honeybees will create male cells a little later in the spaces where there are no additional frames, beeswax male comb foundations, or beeswax male comb foundation frames.

[0049] When there are two frames or male beeswax honeycomb foundations or male beeswax honeycomb foundation frames additionally connected to the lower part of the honeycomb boards, frames and honeycomb foundation frames used in the integrated pest management method using the time-difference mite trap described in claim 2, as shown in part b and part c of Figure 2, each of them is the time-difference mite trap described in claim 6. When there is only one frame or male beeswax honeycomb foundation or male beeswax honeycomb foundation frame additionally connected to the lower part of the honeycomb boards, frames and honeycomb foundation frames used in the integrated pest management method using the time-difference mite trap described in claim 2, that one frame and the space part where no additional installation is performed are each the time-difference mite trap described in claim 7.

[0050] (Claim 3) The time-difference mite trap and the integrated pest management method using the same according to the present invention as described in claim 3 are characterized in that the comb boards and frames and the comb foundation frame used in the integrated pest management method using the time-difference mite trap are comb boards and / or frames and / or comb foundation frames inserted into a honeybee rearing box in beekeeping, the depth dimension of the comb boards and / or frames and / or comb foundation frames is two-thirds to one-third of the depth dimension inside the honeybee rearing box, and the comb foundation frames are comb foundations for female bees, and further, the comb boards and / or frames and / or comb foundation frames are provided with a function for facilitating the production of male honeycomb cells at the bottom. The invention relates to a time-delay mite trap, characterized in that one or more comb frames and / or male beeswax comb bases and / or male beeswax comb frames of any desired size are connected together, and in addition, a container containing a mite-attracting compound, which is an odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation lid is placed near the connection between the comb frames and / or male beeswax comb bases and / or male beeswax comb frames of any desired size and the comb frames and / or male beeswax comb frames of any desired size, and a comb board, frames and comb frames for use in an integrated pest management method using the same.

[0051] In other words, the honeycomb boards, frames and hive foundation frames used in the comprehensive insect pest control method using the time-delay mite trap described in claim 3 have a container containing a mite-attracting compound, which is an odor component of a substance mainly composed of fatty acid esters emitted by honeybee larvae before and after pupation lid, near the connection between the honeycomb boards, frames and hive foundation frames described in claim 1 and the honeycomb frames of any size or male honeycomb foundation made of beeswax or male honeycomb foundation frames made of beeswax described in claim 2 which are connected to the lower part of the honeycomb boards, frames and hive foundation frames described in claim 1.

[0052] As already mentioned, the mite attractant compound is a chemical substance that male bee larvae produce from the body surface just before the cell is covered, and is a substance mainly composed of fatty acid esters and an odorous substance of carboxylic acids and hydrocarbons. Many mites in the breeding box are attracted to this attractant compound and actively invade the frame of any size or the male beehive foundation made of beeswax or the male beehive foundation frame made of beeswax as described in claim 2, which are connected to the lower part of the comb board, frame and foundation frame as described in claim 1. Then, they enter the male beehive cell.

[0053] The attractive effect of the chemical substance emitted from the container containing the mite-attracting compound can be powerfully multiplied as a synergistic effect to attract mites, in addition to the mite-attracting effect of the attractant compound emitted by the male bee larvae in the cells built in the connected comb frames or male beehive foundations made of beeswax as described in claim 2.

[0054] (Claim 4) The time-difference mite trap and integrated pest management method using the same according to the present invention as described in claim 4 is a time-difference mite trap control method that uses as a mite trap the honeycomb boards and / or beehive frames and / or beehive base frames used in the time-difference mite trap and integrated pest management method using the same as described in claim 1, and is an invention of a method characterized in that mites, particularly Varroa destructor mites, can be exterminated by raising honeybees in the order of steps 1 to 5 below. Step 1. One or more beehive boards and / or frames and / or hive foundation frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a first trap on one side of an arrangement of other beehive boards and / or frames and / or hive foundation frames in a beehive (honeybee rearing box). Step 2. In step 1, a male honeycomb is created under the first trap, and the queen bee lays male bee eggs. When the larvae reach the second to fourth instar stages, One or more hive boards and / or frames and / or nest frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a second trap on the other side of the arrangement of other hive boards and / or frames and / or nest base frames in the hive. Step 3. During the pupal period of the male bee larvae in the male honeycomb chamber created at the bottom of the first trap in step 1, the first trap is removed, the male honeycomb chamber created at the bottom of the first trap is cut out, removed and disposed of, and then the first trap is inserted back into its original position. Step 4. During the pupal period of the male bee larvae in the male honeycomb chamber created at the bottom of the second trap in step 2, the second trap is removed, the male honeycomb chamber created at the bottom of the second trap is cut out, removed and disposed of, and then the second trap is inserted in its original position. Step 5. Steps 3 and 4 are repeated.

[0055] The time-delay mite trap and the integrated pest management method using the same according to the present invention described in claim 4 are an invention of a method of mite extermination using a first mite trap and a second mite trap in a time-delay manner, and the method is shown in the description of steps 1 to 5.

[0056] In other words, it is assumed that honeybees are constantly bred in the hive, that a large number of female honeycombs are produced, and that a large number of mites are also present at the same time, and claim 4 is a method for attracting and exterminating the mites. The method uses the comb board or frame or base frame described in claim 1, which is a trap for attracting and exterminating mites, as the first and second mite traps, and aims to continuously attract and exterminate mites in the hive by shifting the periods during which the mite collection and extermination functions of the two mite traps are expressed, providing a time lag.

[0057] In step 1, the comb or frame or the beehive foundation frame described in claim 1 is inserted as a first trap into one end of a hive into which, for example, 10 combs or frames or beehive foundation frames can be inserted. The bees actively start to make male honeycomb cells in the space below the comb or frame or the beehive foundation frame, which is one-third to two-thirds of the hive depth from the bottom of the hive, and the queen bee starts laying male bee eggs, which hatch and grow into larvae.

[0058] Here, one cycle of use of the comb or frame or frame is defined as the period from the time when the comb or frame is inserted into the hive, that is, from the insertion of the first trap, through the formation of male honeycomb cells, queen bee egg laying, male honeycomb larvae hatching, growth from the first instar larvae to the fifth instar larvae, capping of the honeycomb cells, pupation, and the pupal period. Then, about half of the one cycle is the period when the male honeycomb larvae grow and most of the larvae are in the range of the second instar larvae to the fourth instar larvae.

[0059] Insects, including honeybees, grow large and pupate while repeatedly molting after hatching, but the number of molts before pupation is five, including hatching, and in entomology, the larva's growth stage (instar) is expressed by the number of molts. After hatching, the larva becomes a first instar larva (first instar larva), which then molts to become a second instar larva, which then molts again to become a third instar larva, a fourth instar larva, and a fifth instar larva (final instar larva), which then pupates, and after a period as a pupa, emerges as an adult.

[0060] Of course, the many male bee larvae that grow in each of the many male honeycomb cells do not necessarily grow at the same speed at the same time, so the period when most of the larvae grow between the second and fourth instars is set as about half of the period of one cycle mentioned above. And the present invention uses this half period of one cycle, that is, half of one cycle, as the time difference of the mite trap.

[0061] That is, the first first trap is inserted to start one cycle, and the latter half of the cycle is the period during which the first trap functions as a mite trap. The period from when the male bee larvae reach the fifth instar until the cell is covered is the period during which the first trap functions as a mite trap. The next second trap is inserted with a time lag at the same time as the first half of the first cycle of the first trap ends, and one cycle of the second trap begins. Then, the second half of the second cycle of the second trap begins at the same time as the first cycle of the first trap ends, and the second trap functions as a mite trap. Claim 4 shows that by repeating this, the mite trap's function of attracting mites is almost always maintained inside the hive.

[0062] Specifically, in step 1, the first trap is inserted into the hive, and in step 2, halfway through the cycle, the second trap is inserted on the opposite side of the hive. From this time, in the first trap, the period in which male bee larvae emit mite-attracting compounds begins, attracting mites to enter the male bee cell, and the cell is covered just before the larvae pupate. Meanwhile, in the newly inserted second trap, male bee cell formation begins, and then queen bee eggs, male bee larvae hatch, and larval growth begin.

[0063] In step 3, the first trap cycle ends, and the male bee pupae are removed from the first trap while still in the pupal stage, and the male bee cell is cut out and disposed of, including the mites inside. The male bee cell (true trap part) made in the space of one-third to two-thirds of the hive depth dimension at the bottom of the honeycomb board, honeycomb frame or honeycomb foundation frame described in claim 1 is a male bee cell made by beeswax, so it is soft and can be easily cut into any shape with a cutter or the like, and then it is returned to its original position in the hive, and a new cycle begins as the first trap. At the same time, in the second trap, the period when the male bee larvae emit a mite-attracting compound begins, and the mites are attracted and begin to invade the male bee cell. Then, just before the larvae pupate, the nurse bee covers the cell.

[0064] After step 3 is completed, in step 4, when the male honeycomb cell is in the pupal stage, which is the end of one cycle of the second trap, the second trap is removed, and the male honeycomb cell, including the mites inside, is cut out and removed and disposed of. It is then returned to its original position in the hive. At the same time, in the first trap, the male bee larvae begin to emit a mite-attracting compound, and the mites are attracted and begin to invade the male honeycomb cell. Then, just before the larvae pupate, the honeycomb cell is covered with a lid. After that, steps 3 and 4 are repeated every half of the one cycle period. The above is the time-delay type mite trap and the integrated pest management method using the same according to the present invention described in claim 4.

[0065] Here, we will provide additional information on the ecology and habits of honeybees. It is known that male bee larvae attract more Varroa mites than worker bee larvae. By inserting only an empty frame (wooden frame) or a male bee nest into a beehive, a large number of male bee larvae can be obtained in a relatively short time. Mites are attracted to the substance that the male bee larvae excrete before the cells are covered, and they prefer to invade the male bee larvae's cells. When the male bee larvae pupate, the cells are covered, and once all the male bee larvae are covered, the covers can be removed and discarded to attract and exterminate the mites.

[0066] Shortly before the honeybee larvae pupate in the cell, the nurse bee (a worker bee that specializes in raising the young) caps the cell. The amount of capping pheromone released from the surface of the male bee larvae reaches its peak about 10 days after hatching, when they are in the 5th instar stage and shortly before they pupate. This pheromone is said to be the smell of fatty acid esters, carboxylic acids, and hydrocarbons secreted from the surface of the honeybee's body. The nurse bee uses this pheromone to cap the cell of the male bee larvae. Similarly, this pheromone is also an attractant for mites (technically called a kairomone, since the bees that produce the attractant pheromone are harmed in this case), and the mites enter the cell of the male bee larvae just before the capping. When comparing the larvae of female worker bees and male bees, male bee larvae secrete more of this attractant and secrete it for a longer period, so the mites prefer to invade the cells of male bee larvae (Troullier et al., 1992). In honeybees as a whole, the mites reproduce most frequently in the order of male, worker bees, and queen bee. There is a research report (Fuchs S.1992) that the degree to which mites are attracted to the cells of male bee larvae (attraction) is 8 to 12 times that of worker bee larvae. In addition, there is almost no room for the mites to enter and grow in the cells of queen bee larvae. The reason for this is the amount and duration of the pheromone, which is a mite attractant, as well as the short pupal period of worker bees and queen bees after invading the cells. The above is a method of attracting mites using male bee larvae (drone larvae attraction method).

[0067] In addition, the time for which the cells containing bee larvae are covered is 15 to 20 hours for worker cells and 40 to 50 hours for male bee larvae cells, which means that the period during which mites inside the hive can invade and the period during which mites attached to worker bees can invade the cells is longer, and the probability of invading is higher.

[0068] The mites that invade the bee larvae's cell lay eggs, which hatch during the pupal stage when the cell is covered, and grow by parasitizing the pupa. Therefore, the amount of mite reproduction varies depending on the length of the bee's pupal stage. The baby mites increase inside the bee's pupal cell.

[0069] The above is the invention of claim 4, which is a time-difference mite trap control method using as a mite trap the comb boards and / or comb frames and / or comb foundation frames used in the time-difference mite trap and the integrated pest management method using the same described in claim 1. By making good use of the ecology and habits of honeybees and mites, and by continuously using two male honeycomb cell traps to attract mites in a honeybee rearing box (hive), it is possible to attract mites in the hive to the male honeycomb cells with high efficiency, and remove and exterminate them after the cells are covered.

[0070] (Claim 5) The time-difference mite trap and integrated pest management method using the same according to the present invention as described in claim 5 is a time-difference mite trap control method that uses as a mite trap the honeycomb boards and / or beehive frames and / or beehive base frames used in the time-difference mite trap and integrated pest management method using the same as described in claim 1, and is an invention of a method characterized in that mites, particularly Varroa destructor mites, can be exterminated by raising honeybees in the order of steps 1 to 5 below. Step 1. One or more beehive boards and / or frames and / or hive foundation frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a first trap on one side of an arrangement of other beehive boards and / or frames and / or hive foundation frames in a beehive (honeybee rearing box). Step 2. 7 days after step 1, One or more hive boards and / or frames and / or nest frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a second trap on the other side of the arrangement of other hive boards and / or frames and / or nest base frames in the hive. Step 3. 7 days after step 2, The first trap is removed, the male honeycomb chamber formed at the bottom of the first trap is cut out, removed and disposed of, and then the first trap is inserted back into its original position. Step 4. 7 days after step 3, The second trap is removed, the male honeycomb cells formed at the bottom of the second trap are cut out, removed and disposed of, and the second trap is then inserted back into its original position. Step 5. 7 days after step 4, Steps 3 and 4 are repeated every 7 days.

[0071] The time-delay mite trap and integrated pest management method using the same according to the present invention described in claim 5 is an invention of a method of exterminating mites using a first mite trap and a second mite trap in a time-delay manner, similar to claim 4, and the method is described in the description of steps 1 to 5. However, the time-delay mite trap and integrated pest management method using the same according to the present invention described in claim 5 have a noteworthy invention in addition to claim 4. These are described below.

[0072] For example, in early spring, honeybees begin their activity and soon enter the breeding season, which continues for several months as they dramatically increase the number of bee colonies. Within the hive, worker bees are actively creating comb cells, the queen bee lays eggs, nurse bees (worker bees) are raising bee larvae, and worker bees (honeybees) are collecting nectar and beeswax. At the same time, this is the time when mites are actively breeding as bee larvae are raised. This is also the time when beekeepers are extremely busy and tedious with their work of managing the hives and harvesting honey.

[0073] In this breeding season, the worker bees create the cells and the queen bee lays eggs very quickly, and because the cell creation period and the queen bee laying egg period are short, the one cycle period from the insertion of the comb board or frame or the frame as a trap as described above to the formation of the male bee cells, the queen bee laying eggs, the hatching of the male bee larvae, the growth of the first instar larvae to the fifth instar larvae, the closing of the cell, pupation, and the pupal period is also short, and the one cycle period is about 14 days (about 2 weeks). Therefore, the one cycle period is shortened by half, and very conveniently becomes about one week (about 7 days).

[0074] That is, in the time difference type mite trap and the integrated pest management method using the same according to the present invention described in claim 5, the time difference between the first trap and the second trap is one week (7 days), and the mite trap insertion and mite extermination actions from step 1 to step 4 and the repeated actions are performed exactly every 7 days. This is very welcome for beekeepers in the busy season, that is, if step 1 is started on Monday, all the subsequent steps can be performed on the same Monday. For beekeepers who handle dozens or hundreds of hives (breeding boxes), the time difference type mite trap and the integrated pest management method using the same according to the present invention can be performed on the same day of the week determined for each hive, which creates a special and remarkable effect that the work is easy to understand and the complexity is greatly reduced. This effect is a great favorable effect for beekeepers, because if the bee breeding situation is good, it is possible to continue until near August when the honeybee colony calms down. This remarkable effect is a noteworthy invention matter in claim 5.

[0075] (Claim 6) The time-difference mite trap and integrated pest management method using the same according to the present invention as described in claim 6 is a time-difference mite trap control method that uses as a mite trap the honeycomb boards and / or frames and / or base frames used in the time-difference mite trap and integrated pest management method using the same as described in claim 2 or claim 3, and is an invention of a method characterized in that mites, particularly Varroa destructor, can be exterminated by raising honeybees in the order of steps 1 to 5 below. Step 1. A nest board and / or a nest frame and / or a nest base frame for use in the time-difference mite trap and the integrated pest management method using the same according to claim 2 or 3, One of two frames and / or male beehive bases made of beeswax and / or male beehive base frames made of beeswax of any size connected to the bottom of the honeycomb boards and / or frames and / or base frames is designated as the first trap, and the other is designated as the second trap, and is inserted at any position in the arrangement of other honeycomb boards and / or frames and / or base frames in the hive (honeybee rearing box). Step 2. In step 1, male honeycomb cells are created in the first trap and the second trap, and the queen bee lays male bee eggs. When the larvae reach the second to fourth instar stages, The comb boards and / or frames and / or base frames are removed and the male honeycomb cells formed in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 3. During the pupal stage of the male bee larvae in the male beehive chamber created in the first trap, The comb boards and / or frames and / or base frames are removed, the male honeycomb cells formed in the first trap are cut out, removed and disposed of, and then the comb boards and / or frames and / or base frames are reinserted into their original position within the hive. Step 4. During the pupal stage of the male bee larvae in the male beehive chamber created in the second trap, The comb boards and / or frames and / or base frames are removed and the male honeycomb cells formed in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 5. Steps 3 and 4 are repeated.

[0076] The time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 6 are honeycomb boards, frames, or beehive foundation frames used in the time-difference mite trap and the integrated pest management method using the same described in claim 2 or claim 3, and one of two frames, or male honeycomb foundation frames made of beeswax, or male honeycomb foundation frames made of beeswax of any size, connected to the lower part of the honeycomb boards, frames, or beehive foundation frames, is a time-difference mite trap and an integrated pest management method using the same. (See FIG. 2: details will be explained later.)

[0077] That is, the depth dimension of the upper comb board or frame or beehive foundation frame is two-thirds to one-third of the depth dimension inside the honeybee rearing box, and two frames or beeswax male beehive foundations or beeswax male beehive foundation frames are connected to the lower part. Specifically, this is a time-difference mite trap in which one of the two frames or beeswax male beehive foundations or beeswax male beehive foundation frames connected to the lower part is the first trap and the other is the second trap, and an integrated pest management method using the same.

[0078] As described in step 1 of claim 6, the honeycomb board, honeycomb frame, or honeycomb foundation frame used in the time-difference mite trap and the integrated pest management method using the same according to the present invention is only one in total, so it can be inserted at any position in the hive. It can be preferably inserted in the center where mites are easily collected. When a honeycomb foundation is provided, the upper honeycomb foundation (part a in FIG. 2) is a honeycomb foundation for female bees, and the two honeycomb foundations for the trap connected at the bottom (parts b and c in FIG. 2) must be honeycomb foundations for male bees. The size of the honeycomb hexagon of the honeycomb foundation for female bees is smaller than the size of the honeycomb hexagon of the two honeycomb foundations for male bees at the bottom. If there is a container containing the mite attractant compound of claim 3 (part d in FIG. 2), it is more preferable because it can strongly attract and collect mites.

[0079] After step 1, male honeycomb cells are made in the first and second traps connected to the bottom, and the queen bee lays male honeycomb cells, which are then raised by the larvae. When the male honeycomb cells reach the second to fourth instar larvae, step 2 is carried out by removing the honeycomb board, frame or foundation frame, and cutting out and removing all the male honeycomb cells made only in the second trap. The first trap is left as it is, and is reinserted into its original position in the hive. After step 2, the male honeycomb cells in the first trap enter the lid-hanging stage, which serves to attract mites. In the second trap, the male honeycomb cells are made again from the beginning, which marks the beginning of one cycle of the second trap already mentioned, and this is where the time difference between the half cycles of the first and second traps comes from.

[0080] In step 3, during the pupal period of the male bee larvae in the male honeycomb chamber created in the first trap, the comb board or comb frame or comb base frame is removed, the male honeycomb chamber created in the first trap is cut out and removed, and then the comb board or comb frame or comb base frame is reinserted into the original position in the hive. At the same time, the second trap enters the male larvae capping period and exerts the function of attracting mites.

[0081] In step 4, during the pupal period of the male bee larvae created in the second trap, the comb board or comb frame or comb base frame is removed, the male bee cell created in the second trap is cut out and removed and disposed of, and then the comb board or comb frame or comb base frame is reinserted into the original position in the hive. Then, steps 3 and 4 are repeated to successively attract and remove the mites in the hive.

[0082] In other words, the time-delay type mite trap and the integrated pest management method using the same according to the present invention described in claim 6 have a great effect in that both the first trap and the second trap can be created under a single nest board, frame, or nest base frame, and the time lag between each trap can be set to attract and remove mites. In addition, if there is a mite attractant compound container described in claim 3, the mite attractant effect will be outstanding.

[0083] (Claim 7) The time-difference mite trap and integrated pest management method using the same according to the present invention as described in claim 7 is a time-difference mite trap control method that uses as a mite trap the honeycomb boards and / or frames and / or base frames used in the time-difference mite trap and integrated pest management method using the same as described in claim 2 or claim 3, and is an invention of a method characterized in that mites, particularly Varroa destructor, can be exterminated by raising honeybees in the order of steps 1 to 5 below. Step 1. A nest board and / or a nest frame and / or a nest base frame for use in the time-difference mite trap and the integrated pest management method using the same according to claim 2 or 3, A single frame of any size and / or male beehive base made of beeswax and / or male beehive base frame made of beeswax connected to the bottom of the honeycomb boards and / or frames and / or base frames is used as the first trap, and the space below the honeycomb boards and / or frames and / or base frames other than the first trap is used as the second trap, which is inserted at any position in the arrangement of other honeycomb boards and / or frames and / or base frames in the hive (honeybee rearing box). Step 2. In the first trap in step 1, a male honeycomb cell is created, the queen bee lays male bee eggs, and the larvae reach the second to fourth instar stages. The comb boards and / or frames and / or base frames are removed and the male honeycomb cells formed in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 3. During the pupal stage of the male bee larvae in the male beehive chamber created in the first trap, The comb boards and / or frames and / or base frames are removed, the male honeycomb cells formed in the first trap are cut out, removed and disposed of, and then the comb boards and / or frames and / or base frames are reinserted into their original position within the hive. Step 4. During the pupal stage of the male bee larvae in the male beehive chamber created in the second trap, The comb boards and / or frames and / or base frames are removed and the male honeycomb cells formed in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 5. Steps 3 and 4 are repeated.

[0084] The time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 7 are honeycomb boards, frames, or beehive foundation frames used in the time-difference mite trap and the integrated pest management method using the same described in claim 2 or claim 3, and there is only one honeycomb frame, beeswax male honeycomb foundation, or beeswax male honeycomb foundation frame of any size connected to the lower part of the honeycomb board, frame, or beehive foundation frame. This connected honeycomb frame, beeswax male honeycomb foundation, or beeswax male honeycomb foundation frame becomes the first trap, and the remaining space becomes the second trap, in this time-difference mite trap and the integrated pest management method using the same.

[0085] In other words, the depth dimension of the upper honeycomb board or frame or beehive foundation frame is two-thirds to one-third of the depth dimension inside the honeybee rearing box, and only one frame or male honeycomb foundation made of beeswax or male honeycomb foundation frame made of beeswax is connected to the lower part. As in claim 6, this is a time-difference type mite trap and an integrated pest management method using the honeycomb board or frame or beehive foundation frame as a whole as a single honeycomb board or frame or foundation frame for a mite trap.

[0086] As described in step 1 of claim 7, the honeycomb board, honeycomb frame, or honeycomb foundation frame used in the time-difference mite trap and the integrated pest management method using the same according to the present invention is only one as in claim 6, so it can be inserted at any position in the hive. It can be preferably inserted in the center where mites are easily collected. Similarly, when setting up a honeycomb foundation, the upper honeycomb foundation is for female bees, and the honeycomb foundation for the one trap connected at the bottom must be for male bees. As in claim 6, if there is a container containing the mite-attracting compound of claim 3, it is more preferable because it can strongly attract and collect mites.

[0087] After step 1, male honeycomb cells are formed in the first trap connected to the bottom. Similarly, male honeycomb cells are formed in the second trap, which is the space portion, although they tend to be formed later than the first trap. Male honeycomb cells are laid by the queen bee and the larvae are raised. When the male honeycomb larvae in the first trap reach the second to fourth instar larvae, in step 2, the comb board, frame or foundation frame is removed and all male honeycomb cells formed only in the second trap, which is the space portion, are cut out and removed. The first trap is left as it is and is reinserted in its original position in the hive. After step 2, male honeycomb cells in the first trap enter the lid-hanging period and exert their function of attracting mites. In the second trap, which is the space portion, male honeycomb cells begin to be formed again from the beginning, so this is the start of one cycle in the second trap, and this is where the time difference between the first trap and the second trap's half cycle comes from.

[0088] In step 3, during the pupal period of the male bee larvae in the male honeycomb chamber made in the first trap, the comb board or comb frame or comb foundation frame is removed, the male honeycomb chamber made in the first trap is cut out and removed, and then the comb board or comb frame or comb foundation frame is reinserted into the original position in the hive. After that, the male honeycomb chamber made in the second trap, which is the space, enters the lid-covering period and exerts its function of attracting mites.

[0089] In step 4, during the pupal period of the male bee larvae created in the second trap, the comb board or comb frame or comb base frame is removed, the male bee cell created in the second trap is cut out and removed and disposed of, and then the comb board or comb frame or comb base frame is reinserted into the original position in the hive. Then, steps 3 and 4 are repeated to successively attract and remove the mites in the hive.

[0090] That is, the time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 7 create a first trap and a second trap in a single comb board, frame, or beehive foundation frame, but the second trap uses a space that does not require a connecting frame, beeswax male beehive foundation, or beeswax male beehive foundation frame. In other words, there is an effect of reducing the cost and connecting work of one comb frame, beeswax male beehive foundation, or beeswax male beehive foundation frame. For beekeepers, reducing the work is a very welcome effect, and the invention described in claim 7 can achieve this remarkable effect.

[0091] (Claim 8) The time-difference mite trap and integrated pest management method using the same according to the present invention described in claim 8 is an invention of a time-difference mite trap and integrated pest management method using the same, which repeats the steps of each of the time-difference mite trap integrated pest management methods described in claims 4, 5, 6 and 7, and additionally performs steps 1 to 3 below. Step 1. The queen bee in the hive (honeybee rearing box) is isolated by placing it in an isolation cage or a king cage to stop her from laying eggs in the honeycomb cells for about two to three weeks, or the entire hive is moved to a cool environment to stop the queen bee from laying eggs in the honeycomb cells for about two to three weeks. Step 2. For the remaining mite traps, when the male bee larvae turn into operculate larvae, which are male bee pupae with the lids on the cells, the operculate larvae are cut out together with the mites and disposed of. Step 3. Around the 2 to 3 weeks following step 1, use a mite control drug (Apistan, Apivar, Timovar, etc.) according to the instructions for use for a short period of time to eliminate any remaining mites that are parasitizing the worker bees.

[0092] The time-difference mite trap and the integrated pest management method using the same according to the present invention as described in claim 8 are characterized in that they are carried out after carrying out the repeated steps of the integrated pest management methods using the time-difference mite traps as described in claims 4, 5, 6 and 7. Even if the integrated pest management methods using the time-difference mite traps are repeated for several weeks to several months, it is difficult to completely eradicate the mites that are parasitic on the female bees that are worker bees. In addition, as long as the mites that are parasitic on the female bees remain, there is an inherent risk that the reproduction and increase of mites will occur again in the hive after the integrated pest management method using the time-difference mite trap is completed.

[0093] In addition, from the end of July to early August, once the queen bee's egg-laying season is over, the production of male bees also decreases, and the number of male bee larvae cells also decreases, causing the mites to invade the worker bee larvae cells all at once in search of a place to go. If left unchecked, the rapid increase in the mites can lead to the collapse of the bee colony.

[0094] The time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 8 are methods for controlling remaining mites when the number of mites in the hive is reduced to a minimum by carrying out a control method using a time-difference mite trap, and are effective in minimizing the number of mites in the hive. In addition, since the method is a method for controlling the number of remaining mites, the amount of mite control chemicals (registered trademark: Apistan, registered trademark: Apivar, registered trademark: Timovar, etc.) used can be kept to a minimum. This leads to a reduction in the cost of mite control chemicals and the number of operations, and has the advantage of being extremely effective in exterminating mites. In addition, it also leads to an improvement in the quality of the honey collected.

[0095] In step 1, the queen bee is isolated or placed under air conditioning for two to three weeks in order to stop egg laying and eliminate the bee larvae in the cells, which results in no larvae or parasitic mites. Since mites remain in the covered cells in the trap along with the male bee pupae, these are also removed and exterminated, leaving only adult worker bees that may have mites remaining, and then the miticide is applied. In other words, the target of the miticide is limited to the worker bees, and because the worker bees have a high resistance to the miticide, the adverse effects of the miticide on the bee colony and the collected honey can be minimized.

[0096] Incidentally, the above-mentioned method of isolating the queen bee to stop egg-laying is called the queen bee isolation method. It is also called the egg-laying suspension method using a queen cage. A queen cage is a container with a gap that allows only worker bees to pass through, but not the large queen bee.

[0097] The time-difference mite trap and the integrated pest management method using the same according to the present invention described in claim 8 are exactly the idea of ​​integrated pest management (IPM), are the final act of the invention as a method for solving the problems of the present invention, and are aimed at the ultimate extermination of mites. In other words, the time-difference mite trap and the integrated pest management method using the same according to the present invention, which further exterminates remaining mites by the method described in claim 8 after controlling mites by any of the methods described in claims 4 to 7 of the present invention, can be said to be an invention that includes all methods for cutting off mite habitats.

[0098] After the queen bee is released from the cage, she starts laying eggs all at once in the bee colony inside the hive. Egg-laying and rearing during this period leads to the production of bee colonies for overwintering (winter bees: bees whose bodies have been prepared for overwintering as pupae, also known as winter bees), so controlling and eliminating mites greatly contributes to ensuring healthy bees suitable for overwintering.

[0099] It goes without saying that the time and timing of the integrated pest management (IPM) method, which combines the time-delayed mite trapping method with isolating the queen bee in a separate king cage and using a mite extermination drug, must be adjusted according to the seasonal environment of the region where the beekeeping is carried out. It is clear that the effect of the present invention can be maximized by performing the time-delayed mite trapping method, isolating the queen bee in a separate king cage and using a mite extermination drug in conjunction with the ecological behavior of honeybees, such as the start of their activity, peak period, resting period, winter bee production period, and overwintering period.

[0100] As described above, the time-difference mite trap and the integrated pest management method using the same according to the present invention have been described in claims 1 to 8 as a means for solving the above problems, but the present invention is not limited to the above-mentioned embodiment, and can be modified and implemented within the scope of the gist of the present invention. All of these suitable forms for each application are included in the technical spirit of the present invention. It is also asserted that all equivalent methods using prior art are included in the technical spirit of the present invention. Effect of the Invention

[0101] In other words, the present invention provides the development of a beekeeping technique for effectively comprehensively eradicating and controlling Varroa mites in the beekeeping industry in a non-complex and as simple a manner as possible, without requiring the beekeeper to do any labor or labor, without requiring the installation of mechanical devices, and by minimizing the use of chemical substances such as pesticides, thereby collecting pure honey with minimal impurities and chemical contamination. The present invention is a mite extermination method that makes maximum use of the natural ecology and habits of honeybees and mites, and can also minimize adverse effects on the environment. In addition, the present invention also provides a method for solving the above problems based on the concept of Integrated Pest Management (IPM) to minimize the use of pesticides. [Brief description of the drawings]

[0102] [Figure 1]This is a schematic perspective view showing an overall image of a typical honeybee hive (breeding box). [Diagram 2] This is a conceptual diagram showing an overall image of a honeycomb board, frame, or base frame according to one embodiment of the invention described in claims 2 and 3 of the present invention. [Diagram 3] FIG. 5 is a conceptual diagram showing each step according to one embodiment of the method according to claim 5 of the present invention. [Figure 4] FIG. 1 is a conceptual diagram showing an overall image of the schedule of each step according to one embodiment of the method according to claim 5 of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0103] Hereinafter, the embodiment of the present invention will be described with reference to the drawings. Note that, in the following, the scope necessary for the explanation to achieve the object of the present invention is shown in a schematic manner, and the scope necessary for the explanation of the relevant part of the present invention will be mainly explained, and the parts that are omitted from the explanation will be referred to as publicly known techniques.

[0104] FIG. 1 is a perspective conceptual diagram showing an overall image of a typical beehive (breeding box). As shown in the figure, the comb boards, frames, or comb foundation frames inserted into the beehive 1 are generally composed of comb boards 2, frames 3, and comb foundations 4. The comb boards 2 are sometimes called comb boards 2 including the frames 3, comb foundations 4, and the honeycomb cells that have been created. The comb frames 3 are a board frame that covers the outer periphery. The comb foundations 4 are thin plates made of beeswax that are stamped with a honeycomb shape (hexagonal shape) to make it easier for bees to create honeycomb cells, and are also made of plastic. The honeycomb sizes of the comb foundations 4 range from small ones for female bee larvae to slightly larger ones for male bee larvae. The comb frames 3 with the comb foundations 4 installed are called comb foundation frames.

[0105] As shown in Fig. 1, generally, the honeycomb boards 2 or the beehive frames 3 or the beehive foundation frames have a depth dimension 5 which is the inner depth dimension of the hive 1, a width dimension 6 which is the inner width dimension of the hive 1, and a width dimension 7 which is the dimension that allows about 10 of them to fit within the inner length dimension of the hive 1. The beehive entrance 9 is the entrance and exit for the honeybees, and the inside of the hive is sealed except for the entrance 9.

[0106] In the honeycomb boards 2 or frames 3 or foundation frames according to an embodiment of the invention described in claim 1 of the present invention, only the depth dimension of the honeycomb boards 2 or frames 3 or foundation frames shown in FIG. 1 is approximately two-thirds to one-third of the internal depth dimension of the hive. When the honeycomb foundation 3 is used, it is a honeycomb foundation for worker bees (female bees). This honeycomb foundation for female bees may be made of beeswax or plastic. This is to make the honeycomb foundation for female bees make female honeycomb cells, create a state where there are no male honeycomb cells, and actively make honeycomb cells for male bees in the lower space.

[0107] Normally, a bee colony may build male cells anywhere on the comb, frame or foundation of a hive, but if a comb, frame or foundation is provided that induces the bee colony to build male cells in a specific location, the bee colony will prefer to build male cells there and will not build male cells in other locations. The mite trap of the present invention utilizes this behavior.

[0108] Fig. 2 is a conceptual diagram showing an overall image of a honeycomb board or frame or a honeycomb foundation frame according to an embodiment of the invention described in claim 2 and claim 3 of the present invention. As shown in the figure, the upper part a of the honeycomb board or frame or a honeycomb foundation frame according to an embodiment of the invention described in claim 2 of the present invention is a honeycomb foundation for female bees, and allows female beehives to create honeycomb cells. Although the present invention also includes cases where there is no honeycomb foundation, basically the upper part a is used to allow female beehives to create honeycomb cells. The honeycomb foundation for female bees in part a may be made of beeswax or plastic.

[0109] As shown in Fig. 2, parts b and c are frames of any size or male beehive foundations made of beeswax or male beehive foundation frames made of beeswax as described in claims 2 and 3, and in the case of Fig. 2, parts b and c are connected to part a. However, parts b and c do not necessarily have to be connected. Although it is difficult to see in Fig. 2, part a is a foundation for female worker bees, so the hexagonal size of the honeycomb is smaller than the hexagonal size of the honeycomb of parts b and c for male beehive foundations. In the case of Fig. 2, it is used in the method described in claim 6 of the time-difference mite trap and the integrated pest management method using the same according to the present invention.

[0110] Parts b and c of FIG. 2 depict male honeycomb frames made of beeswax, but of course parts b and c also include within the present invention cases in which only the frames 3 are used without using male honeycomb frames made of beeswax.

[0111] The time-difference mite trap and the integrated pest management method using the same according to the present invention, as described in claim 7, only have parts a and b in Fig. 2, and part c is left as a space. The time-difference mite trap and the integrated pest management method using the same according to the present invention, as described in claim 2, does not have part d in Fig. 2, and the invention described in claim 3, as has been added part d in Fig. 2.

[0112] Part d in Fig. 2 shows an example of a container containing a mite attractant compound, which is an odor component of a substance mainly composed of fatty acid ester emitted by honeybee larvae before and after pupation capping, as described in claim 3 of the time-difference mite trap and the integrated pest management method using the same according to the present invention. Of course, part d of the container containing the mite attractant compound may have a structure that allows the mite attractant compound, which is an odor component of a substance mainly composed of fatty acid ester, to be emitted outside the container.

[0113] FIG. 3 is a conceptual diagram showing each step according to one embodiment of the method according to claim 5 of the present invention. It is a conceptual diagram showing the honeycomb boards in the hive from the side. As shown in FIG. 3, 10 honeycomb boards 2 or frames 3 or foundation frames are inserted inside the hive 1, and the honeycomb boards or frames or foundation frames having the trap A part and the honeycomb boards or frames or foundation frames having the trap B part are honeycomb boards or frames or foundation frames described in claim 1, respectively, and the depth dimension is two-thirds to one-third of the depth dimension inside the hive. And, the A part and the B part are the parts where the worker bees make male honeycomb cells to become mite traps.

[0114] In other words, Figure 3 is a diagram showing one embodiment of a time-difference mite trap control method that uses a nest board, hive frame, or nest foundation frame used in the time-difference mite trap described in claim 1 and the integrated pest management method using it as a mite trap, and is a time-difference mite trap described in claim 5 and the integrated pest management method using it. As shown in FIG. 3, each step according to an embodiment of the method according to claim 5 of the present invention is described below.

[0115] Step 1, As shown in step 1 of Figure 3, the first trap board A, which is made by cutting off the lower part of a honeycomb board, frame, or foundation frame, is inserted into the left outside of the hive of the bee colony. (Hereinafter, the honeycomb board, frame, or foundation frame having part A that will become the first trap will be referred to as the "first trap board A," and the honeycomb board, frame, or foundation frame having part B that will become the second trap will be referred to as the "second trap board B.") If the colony is in its prime, a male cell will soon be created in the cut-out space A, and at the same time, the queen bee will lay eggs that will become male bees. The queen bee can lay either male or female (worker) eggs depending on the size of the cell. Step 2, One week later (week 2), a second similarly prepared trap plate B is inserted on the outside of the opposite side. Step 3, Two weeks later (week 3), the first trap panel A is removed and the male bee larvae have grown in the male beehive chambers in part A and the chambers are now covered. The male beehive chambers are cut off and disposed of. In other words, the male beehive chambers that have attracted and invaded in large numbers are exterminated. The first trap panel A with the male beehive chambers cut off and removed is then reinserted in the same place. Step 4, Three weeks later (week 4), cut off the capped male honeycomb chamber (the capped male honeycomb chamber of the male honeycomb) at the bottom of the second trap board B in the same way. In other words, remove the male honeycomb chamber that had attracted a large number of mites. Then, return the second trap board B to its original place in the same way. Step 5, Repeat steps 3 and 4 above.

[0116] As a result, the male honeycomb cells that attract and invade a large number of mites are constantly cut off, resulting in continuous mite extermination. In other words, the first trap nest board A and the second trap nest board B are mite attracting traps for mite extermination. This method makes it possible to keep the mite traps always installed in the hive, effectively minimizing the damage caused by mites.

[0117] As shown above with reference to FIG. 3, the invention of the method according to claim 5 of the time-difference type mite trapping method of the present invention shows that the period from the first inserted trap board to the next trap board is one week, then the period from the first inserted trap board to the cutting and removal of the trap part and reinsertion is another week, and then the period from the cutting and removal of the trap part and reinsertion of the next trap board is another week, repeating the work in one-week units. This is devised because the period for making male nest cells and the period for the development of male bee larvae can be conveniently adjusted in one-week units from the start of the spring activity of the bee colony to its peak. However, it should be noted that the time-difference type mite trapping method of the present invention other than claim 5 is not limited to this one-week unit. The length of this work unit can be adjusted according to the growth condition of the honeybees in the present invention.

[0118] The unit period of the repetitive work varies depending on various factors, such as the number of bees in the colony, their activity status, seasonal changes, whether the region is warm or cold, the activity status of the queen bee, etc. In other words, the period of the repetitive work in the time-delay type mite trap extermination method according to the present invention is not limited to one week, except for claim 5, but can be adjusted depending on the colony and beekeeping status at that time, and conversely, it is confirmed and described that the period should be adjusted taking into account various factors.

[0119] Incidentally, if a plastic male bee foundation is used as the foundation for the mite trap, not only is it difficult to cut it off during post-trap processing, but it is also necessary to use a high-pressure washer or the like, which is a very time-consuming task. For example, inserting an empty foundation (e.g., a wire frame) to create a male bee cell is a simpler task, since it only requires cutting off the beehive made of beeswax. In addition, the foundations described in claims 2 and 3 of the present invention have the advantage that the foundations are made of beeswax, so they can be similarly cut off, and because they are provided with a male bee foundation, the worker bees can quickly and reliably create male bee cells as mite traps.

[0120] In August, after the peak of the queen bee's activity, the colony's momentum of building male honeycomb cells in the trap board slows down, and they may start building worker honeycomb cells in the lower part of the trap board, so in that case, the number of trap boards may be reduced from the above two A and B to one. Conversely, during the peak of the queen bee and the peak of the colony, the number of trap boards may be increased, for example to two each of A and B.

[0121] FIG. 4 is a conceptual diagram showing an overall schedule image of each step according to one embodiment of the method according to claim 5 of the present invention. In other words, a method of periodically performing a time-difference mite extermination method using a mite trap is simulated. As shown in the figure, the upper column of the figure lists the schedule and days of the week from April in spring, which is the peak season for honeybees. The middle column shows the growth cycle from trap insertion of the first trap used in the time-difference mite trap extermination method according to claim 5 of the present invention to egg-laying, hatching, and pupation of male honeybees. Similarly, the lower column shows the growth cycle from trap insertion of the second trap used in the time-difference mite trap extermination method according to claim 5 of the present invention to egg-laying, hatching, and pupation of male honeybees.

[0122] As shown in FIG. 4, one cycle of eggs-laying, hatching, and pupation by male bees in the first trap and one cycle of eggs-laying, hatching, and pupation by male bees in the second trap were repeated with a time lag of seven days.

[0123] The part marked "trap" in Figure 4 indicates the time (period) when male bee larvae in male beehive cells designed as mite traps pupate, releasing a large amount of capping pheromone, and mites (mainly female mites) are attracted to the capping pheromone of the male bee larvae and enter the male bee larvae cells. Since the first and second traps repeat the growth cycle of male bee larvae in a time-delayed manner, the period when the capping pheromone of the larvae that attracts the above-mentioned mites is released is repeated almost continuously within the hive. Therefore, it is possible to constantly attract and exterminate mites within the hive.

[0124] To reiterate, the above-mentioned "male bee larvae operculum-closing pheromone" refers to a mite-attracting compound, which is an odor component of a substance mainly composed of fatty acid esters, emitted by honeybee larvae before and after pupation operculum-closing, as described in claim 3 of the present invention.

[0125] When the time-difference mite trap removal work is performed as shown in Figure 4, the mite trap insertion and mite removal work can be performed regularly and continuously on the same day of the week in units of one week without forgetting, which is very simple and convenient for beekeepers. In addition, if a simple comb or frame or foundation (foundation frame) such as the lower cut comb described in claim 1 is used as a mite trap, it becomes an inexpensive mite trap that does not cost much and is very meaningful economically. In addition, the work of cutting out the male bee larvae nest chamber part formed in the lower space part of the cut comb for the mite trap is the work of cutting out the honeycomb including the comb chamber made of beeswax, so it can be easily cut and removed with a utility knife or the like, which is simple and very convenient.

[0126] During the period when this time-delayed mite trapping method is continued, the occurrence of mites is minor, and there is no need to use pesticides, resulting in a significant reduction in pesticide costs. For large-scale beekeepers who operate with dozens to hundreds of hives, the above method is a simple, highly effective and meaningful mite trapping method.

[0127] In the peak season of spring, some bee colonies may make nest cells over the entire surface of the comb in one night, and male eggs may be laid there. In this case, the male bee larvae in the comb grow and pupate at the same time, so the effective period of the mite trap, which is the period until the comb cells are covered at the same time, is shortened to 2 to 3 days. However, although there are cases where the time is accelerated, on the other hand, the period for making the male comb cells is delayed, the period for the growth of the male larvae also varies, and the period for covering the comb cells is prolonged, that is, the trap period is extended. In this case, such a period or date may be predicted, and only the period for inserting the comb board into the mite trap and the period for cutting the comb cells to cover the male bee larvae may be shortened or extended to adjust the period. Whether the above-mentioned repetition period is short or long, the purpose of the time-difference mite trap and the integrated pest management method using the same according to the present invention is the same, and these responses are included in the technical idea of ​​the present invention.

[0128] In addition, in beekeeping in warm regions, for example, the queen bee may not stop laying eggs even in December, so in this case, bee larvae will also be present and the reproduction of mites will continue. Therefore, if the queen bee is not isolated during this period to stop laying eggs, it is extremely effective to use the time-delayed mite trap extermination method of the present invention in consideration of the above-mentioned repetition period adjustment in order to exterminate mites.

[0129] As described above, the present invention is not limited to the above-mentioned embodiment, and various modifications can be made without departing from the spirit of the present invention. However, all such modifications are included in the technical concept of the present invention.

[0130] All suitable forms for different applications similar to the present invention are included in the technical spirit of the present invention. We also assert that equivalent methods using prior art are included in the technical spirit of the present invention. [Industrial Applicability]

[0131] As described above, according to the present invention, it is possible to provide and realize the development of beekeeping technology in the beekeeping industry that effectively performs comprehensive extermination and control of Varroa mites using a simple method that avoids complexity, without requiring much effort, and without the expense and energy required for installing mechanical devices, and in addition, by minimizing the use of chemical substances such as pesticides, and that allows for the collection of pure honey with minimal impurities and chemical contamination.

[0132] Furthermore, the present invention is a method for exterminating mites that makes maximum use of the natural ecology and habits of honeybees and mites, and can minimize adverse effects on the environment. In addition, the present invention can provide society with the concept of Integrated Pest Management (IPM), which minimizes the use of extermination chemicals.

[0133] Furthermore, the present invention can be used and applied not only to the beekeeping of Western honeybees, but also to the beekeeping of any type of honeybee. Therefore, the present invention will be of great benefit not only to the beekeeping industry, but also to various industries that use honey as a raw material. [Explanation of symbols]

[0134] 1. Beehive (honeybee breeding box) 2 nest board 3 Nest frame 4 Nest foundation 5 Depth dimension 6. Horizontal dimensions 7 Width Dimensions 8 Length dimensions 9 Nest Gate 10. Honeycomb boards, frames, and nest bases (nested nest base frames) according to the present invention 11 First trap nest plate A 12 Second trap nest board B 13 Mites (Varroa destructor)

Claims

1. A comb board and / or a frame and / or a base frame that are inserted into a beekeeping box in beekeeping, The depth dimension of the honeycomb board and / or the beehive frame and / or the beehive foundation frame is two-thirds to one-third of the depth dimension inside the honeybee rearing box, and the beehive foundation frame is a beehive foundation for female bees, The lower part of the honeycomb boards and / or frames and / or base frames is made easy for worker bees to make male honeycomb cells; A time-difference type mite trap characterized by the above features and a nest board, a nest frame and a nest base frame for use in an integrated pest management method using the same.

2. A comb board and / or a frame and / or a base frame that are inserted into a beekeeping box in beekeeping, The depth dimension of the honeycomb board and / or the beehive frame and / or the beehive foundation frame is two-thirds to one-third of the depth dimension inside the honeybee rearing box, and the beehive foundation frame is a beehive foundation for female bees, Furthermore, one or more frames and / or male honeycomb foundations made of beeswax and / or male honeycomb foundation frames made of beeswax of any size are provided in a connected manner at the lower part of the honeycomb boards and / or frames and / or foundation frames to facilitate the production of male honeycomb cells. A time-difference type mite trap characterized by the above features and a nest board, a nest frame and a nest base frame for use in an integrated pest management method using the same.

3. A comb board and / or a frame and / or a base frame that are inserted into a beekeeping box in beekeeping, The depth dimension of the honeycomb boards and / or frames and / or honeycomb foundation frames is two-thirds to one-third of the depth dimension inside the honeybee rearing box, and the honeycomb foundation is a honeycomb foundation for female bees, Furthermore, one or more frames and / or beeswax male honeycomb foundations and / or beeswax male honeycomb foundation frames of any size are connected to the lower part of the honeycomb boards and / or frames and / or beeswax male honeycomb foundation frames to facilitate the production of male honeycomb cells; In addition, a container containing a mite attractant compound, which is an odor component of a substance mainly composed of fatty acid ester emitted by honeybee larvae before and after pupation, is provided near the connection between the honeycomb board and / or the honeycomb frame and / or the honeycomb base frame and the honeycomb frame of any size and / or the male honeycomb base made of beeswax and / or the male honeycomb base frame made of beeswax; A time-difference type mite trap characterized by the above features and a nest board, a nest frame and a nest base frame for use in an integrated pest management method using the same.

4. A time-difference mite trap control method using the honeycomb board and / or beehive frame and / or beehive base frame used in the time-difference mite trap and the integrated pest management method using the same as described in claim 1 as a mite trap, and by raising honeybees in the order of steps 1 to 5 below, mites, particularly Varroa destructor, can be exterminated. A time-difference type mite trap and an integrated pest management method using the same. Step 1. One or more beehive boards and / or frames and / or hive foundation frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a first trap on one side of an arrangement of other beehive boards and / or frames and / or hive foundation frames in a beehive (honeybee rearing box). Step 2. In step 1, a male honeycomb is formed at the bottom of the first trap, and the queen bee lays male bee eggs. When the larvae reach the second to fourth instar stages, One or more beehive boards and / or frames and / or nest base frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a second trap on the other side of the arrangement of other beehive boards and / or frames and / or nest base frames in the hive. Step 3. During the pupal period of the male bee larvae in the male honeycomb chamber created under the first trap in step 1, the first trap is removed, the male honeycomb chamber created under the first trap is cut out, removed and disposed of, and then the first trap is inserted back into its original position. Step 4. During the pupal period of the male bee larvae in the male honeycomb chamber created at the bottom of the second trap in step 2, the second trap is removed, the male honeycomb chamber created at the bottom of the second trap is cut out, removed and disposed of, and then the second trap is inserted back into its original position. Step 5. Steps 3 and 4 are repeated.

5. A time-difference mite trap control method using the honeycomb board and / or beehive frame and / or beehive base frame used in the time-difference mite trap and the integrated pest management method using the same as described in claim 1 as a mite trap, and by raising honeybees in the order of steps 1 to 5 below, mites, particularly Varroa destructor, can be exterminated. A time-difference type mite trap and an integrated pest management method using the same. Step 1. One or more beehive boards and / or frames and / or hive foundation frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a first trap on one side of an arrangement of other beehive boards and / or frames and / or hive foundation frames in a beehive (honeybee rearing box). Step 2. 7 days after step 1, One or more beehive boards and / or frames and / or nest base frames used in the time-delay mite trap and the integrated pest management method using the same described in claim 1 are inserted as a second trap on the other side of the arrangement of other beehive boards and / or frames and / or nest base frames in the hive. Step 3. 7 days after step 2, The first trap is removed, the male honeycomb cells formed at the bottom of the first trap are cut out, removed and disposed of, and the first trap is then inserted back into its original position. Step 4. 7 days after step 3, The second trap is removed and the male honeycomb cells formed at the bottom of the second trap are cut out, removed and disposed of, after which the second trap is inserted back into its original position. Step 5. 7 days after step 4, Steps 3 and 4 are repeated every 7 days.

6. A time-difference mite trap control method using a honeycomb board and / or a beehive frame and / or a beehive base frame used in the time-difference mite trap and the integrated pest management method using the same as described in claim 2 or claim 3 as a mite trap, and by raising honeybees in the order of steps 1 to 5 below, mites, particularly Varroa destructor, can be exterminated. A time-difference type mite trap and an integrated pest management method using the same. Step 1. A nest board and / or a nest frame and / or a nest base frame for use in the time-difference mite trap and the integrated pest management method using the same according to claim 2 or 3, One of two frames and / or male honeycomb foundations made of beeswax and / or male honeycomb foundation frames made of beeswax of any size connected to the lower part of the honeycomb boards and / or frames and / or base frames is used as a first trap, and the other is used as a second trap, and is inserted at any position in the arrangement of other honeycomb boards and / or frames and / or base frames in the hive (honeybee rearing box). Step 2. In step 1, male honeycomb cells are created in the first trap and the second trap, and the queen bee lays male bee eggs. When the larvae reach the second to fourth instar stages, The comb boards and / or frames and / or base frames are removed and the male honeycomb cells produced in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 3. During the pupal stage of the male bee larvae in the male beehive chamber created in the first trap, The honeycomb boards and / or frames and / or base frames are removed, the male honeycomb cells produced in the first trap are cut out, removed and disposed of, and then the honeycomb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 4. During the pupal stage of the male bee larvae in the male beehive chamber created in the second trap, The comb boards and / or frames and / or base frames are removed and the male honeycomb cells produced in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 5. Steps 3 and 4 are repeated.

7. A time-difference mite trap control method using a honeycomb board and / or a beehive frame and / or a beehive base frame used in the time-difference mite trap and the integrated pest management method using the same as described in claim 2 or claim 3 as a mite trap, and by raising honeybees in the order of steps 1 to 5 below, mites, particularly Varroa destructor, can be exterminated. A time-difference type mite trap and an integrated pest management method using the same. Step 1. A nest board and / or a nest frame and / or a nest base frame for use in the time-difference mite trap and the integrated pest management method using the same according to claim 2 or 3, A single beehive frame of any size and / or a male beehive base made of beeswax and / or a male beehive base frame made of beeswax connected to the lower part of the honeycomb boards and / or frames and / or beehive foundation frames is used as a first trap, and the space below the honeycomb boards and / or frames and / or beehive foundation frames other than the first trap is used as a second trap, which is inserted at any position in the arrangement of other honeycomb boards and / or frames and / or beehive foundation frames in the hive (honeybee rearing box). Step 2. In the first trap of step 1, a male honeycomb cell is created, the queen bee lays male bee eggs, and the larvae reach the second to fourth instar stages. The comb boards and / or frames and / or base frames are removed and the male honeycomb cells produced in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 3. During the pupal stage of the male bee larvae in the male beehive chamber created in the first trap, The honeycomb boards and / or frames and / or base frames are removed, the male honeycomb cells produced in the first trap are cut out, removed and disposed of, and then the honeycomb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 4. During the pupal stage of the male bee larvae in the male beehive chamber created in the second trap, The comb boards and / or frames and / or base frames are removed and the male honeycomb cells produced in the second trap are cut out, removed and disposed of, after which the comb boards and / or frames and / or base frames are reinserted into their original positions within the hive. Step 5. Steps 3 and 4 are repeated.

8. After repeating the steps of each of the time-difference mite trap integrated pest management methods described in claims 4, 5, 6 and 7, a time-difference mite trap and an integrated pest management method using the same are additionally carried out in steps 1 to 3 below. Step 1. The queen bee in the hive (honeybee rearing box) is isolated by placing it in an isolation cage or a king cage to stop egg-laying in the honeycomb cells for about two to three weeks, or the entire hive is moved to a cool environment to stop the queen bee from egg-laying in the honeycomb cells for about two to three weeks. Step 2. The remaining mite traps are used to cut out and remove the mites together with the male bee larvae that become operculate larvae, which are male bee pupae with the lids placed on the cells of the nest, and then disposed of. Step 3. Around the 2 to 3 weeks of step 1, a mite extermination drug (Apistan, Apivar, Timovar, etc.) is used for a short period of time according to the instructions to exterminate the remaining mites that are parasitizing the worker bees.

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