Ecotoxicity monitoring device using luminescent bacterium and ecotoxicity monitoring method using the same

Abstract

To provide an ecotoxicity monitoring device using a luminescent bacterium and an ecotoxicity monitoring method using the same.SOLUTION: An ecotoxicity monitoring device using a luminescent bacterium and an ecotoxicity monitoring method using the same are provided. The ecotoxicity monitoring device comprises: a reaction tank module including a reference reaction tank, a sample reaction tank, and a dilution reaction tank, and supplies a mixed solution containing the luminescent bacterium; a luminescent bacterium culture portion; a diluted water supply portion; a sample supply portion; an optical measurement portion that measures the amount of luminescence of the luminescent bacterium in a toxicity assessment substance provided from the sample reaction tank and the dilution reaction tank; a control portion; and an analysis portion that determines whether a toxic substance is present a sample on the basis of a light quantity value provided from the optical measurement portion. After time predetermined as an initial light quantity value of the reference reaction tank measured through the optical measurement portion has elapsed, the analysis portion is configured to calculate a correction coefficient through the measured final light quantity value, and calculate a corrected light quantity value of the sample reaction tank and dilution reaction tank through the calculated correction coefficient.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to an ecotoxicity monitoring device using luminescent bacteria and an ecotoxicity monitoring method using the same, and more particularly to an ecotoxicity monitoring device using luminescent bacteria that is adapted to quickly and accurately monitor the presence or absence of toxicity in a sample and an ecotoxicity monitoring method using the same. [Background technology]

[0002] Although sewage and wastewater treatment facilities have been established and operated to improve water quality and protect the ecosystem, there is a problem that harmful chemicals cannot be completely removed through biological or physicochemical treatment processes. Therefore, harmful chemicals that are not removed during sewage and wastewater treatment are discharged into public water bodies, causing adverse effects on the ecosystem and health.

[0003] In addition, hazardous / noxious substances (HNS) discharged into the ocean due to marine industrial facilities or ship accidents cause direct and indirect damage to the surrounding ocean and seabed, marine users, and coastal residents. Such hazardous / noxious substances discharged into the ocean are diverse, and physicochemical analysis alone without prior information provides limited information on the types and characteristics of substances. In addition, physicochemical analysis techniques are practically impossible to analyze toxic substances in all samples due to the cost, time, technology, and manpower required for analysis.

[0004] Therefore, ecotoxicity assessment and analysis techniques are emerging as a scientific and efficient management method for environmental analysis. These ecotoxicity assessment and analysis techniques use living organisms to evaluate the toxicity of substances present in the environment.

[0005] However, the conventional ecotoxicity evaluation device has a problem that it takes at least 15 minutes to evaluate whether or not a toxic substance exists in a sample, even if it is the fastest. As described above, the longer the evaluation time is when evaluating whether or not a toxic substance exists in a sample using the ecotoxicity evaluation device, the more difficult it is to respond quickly to environmental pollution, and the greater the damage becomes.

[0006] Therefore, various research and development efforts are being conducted on ecotoxicity monitoring devices that can more quickly and accurately monitor hazardous / harmful substances that are discharged into not only rivers but also the ocean. [Prior art documents] [Patent documents]

[0007] [Patent Document 1] Korean Patent Publication No. 2014-0093389 (2014.07.28) Summary of the Invention [Problem to be solved by the invention]

[0008] The technical objective of the present invention to solve the above problems is to provide an ecotoxicity monitoring device using luminescent bacteria that can quickly and accurately monitor the presence or absence of toxicity in a sample, and an ecotoxicity monitoring method using the same. [Means for solving the problem]

[0009] In order to achieve the above technical objectives, one embodiment of the present invention provides a reaction vessel module including a reference reaction vessel, a sample reaction vessel, and a dilution reaction vessel, the reaction vessel module including a bacterial dilution vessel that supplies a mixture containing luminescent bacteria to the reference reaction vessel, the sample reaction vessel, and the dilution reaction vessel; a luminescent bacteria culturing unit that supplies luminescent bacteria to the bacterial dilution vessel for detecting the presence or absence of toxicity of a sample; a dilution water supplying unit that supplies dilution water to the bacterial dilution vessel, the reference reaction vessel, and the dilution reaction vessel; a sample supplying unit that supplies a sample to the sample reaction vessel and the dilution reaction vessel; and an analysis unit that determines whether or not a toxic substance is present in a sample based on a light intensity value provided by the optical measurement unit, wherein the analysis unit calculates a correction coefficient based on an initial light intensity value of the reference reaction tank measured by the optical measurement unit and a final light intensity value measured after a predetermined time has elapsed, and calculates corrected light intensity values ​​of the sample reaction tank and the dilution reaction tank based on the calculated correction coefficient.

[0010] In one embodiment of the present invention, the analysis unit is configured to calculate the toxicity index (TI s ) is obtained, but the toxicity index (TI s ) is calculated by calculating the correction coefficient of the reference reaction vessel according to the following formula 1, calculating the corrected light intensity value of the sample reaction vessel to which the correction coefficient is applied according to the following formula 2, and calculating the toxicity index (TI) based on the final light intensity value of the sample reaction vessel measured after a predetermined time has elapsed after a required sample is injected from the sample supply unit into the sample reaction vessel according to the following formula 3. s ) can be calculated.

[0011]

number

[0012]

number

[0013] [S0 = initial light intensity value after 1 minute of the mixture supplied from the bacteria dilution chamber to the sample reaction chamber, C0 = correction coefficient]

[0014]

number

[0015] [S1 = corrected light intensity value after the first minute of the sample reaction tank to which the correction coefficient has been applied, S2 = final light intensity value after a predetermined time has elapsed with a fixed amount of sample injected into the mixed solution in the sample reaction tank]

[0016] In one embodiment of the present invention, the analysis unit is configured to calculate the toxicity index (TI d ) is obtained, but the toxicity index (TI d ) is calculated by calculating the corrected light intensity value of the dilution reaction vessel to which the correction coefficient of the reference reaction vessel is applied according to the following formula 4, and the toxicity index (TI) is calculated based on the final light intensity value of the dilution reaction vessel measured after the sample and dilution water are injected into the dilution reaction vessel at a required ratio from the sample supply unit and the dilution water supply unit according to the following formula 5, after a predetermined time has elapsed. d ) can be calculated.

[0017]

number

[0018] [D0 = initial light intensity value after 1 minute of the mixture supplied from the bacterial dilution tank to the dilution reaction tank, C0 = correction coefficient]

[0019]

number

[0020] [D1 = corrected light intensity value after the first minute of the dilution reaction tank to which the correction coefficient has been applied, D2 = final light intensity value after a predetermined time has elapsed with a fixed amount of sample and dilution water injected into the mixture in the dilution reaction tank]

[0021] In one embodiment of the present invention, the analysis unit determines that the calculated correction coefficient is valid when the calculated correction coefficient is between 0.6 and 1.3, and sets the calculated toxicity index (TI s ) is between 20 and 80%, it can be determined that toxic substances are present in the sample.

[0022] In one embodiment of the present invention, the analysis unit is configured to calculate the toxicity index (TI s ) and the toxicity index (TI d ) the toxicity risk of the sample can be assessed.

[0023] In one embodiment of the present invention, the predetermined time for measuring the final light intensity values ​​of the reference reaction chamber, the sample reaction chamber and the dilution reaction chamber may be 5 minutes.

[0024] One embodiment of the present invention includes the steps of: (A) preparing a mixture of luminescent bacteria and dilution water in a bacteria dilution tank; (B) supplying the mixture from the bacteria dilution tank to a reference reaction tank, a sample reaction tank and a dilution reaction tank, respectively; (C) measuring initial light quantities of the luminescent bacteria in the reference reaction tank, the sample reaction tank and the dilution reaction tank through an optical measurement unit after a predetermined time has elapsed; (D) supplying dilution water to the reference reaction tank, supplying a sample to the sample reaction tank and supplying a predetermined amount of the sample and dilution water to the dilution reaction tank after measuring the initial light quantities; and (E) measuring the initial light quantities of the luminescent bacteria in the reference reaction tank, the sample reaction tank and the dilution reaction tank through the optical measurement unit after a predetermined time has elapsed. and (F) providing the initial and final light values ​​of the luminescent bacteria in the reference reaction chamber measured by the optical measurement unit to an analysis unit, which determines whether or not a sample has toxicity. The analysis unit calculates a correction coefficient based on the initial and final light values ​​of the reference reaction chamber measured by the optical measurement unit, calculates corrected light values ​​of the sample reaction chamber and the dilution reaction chamber based on the calculated correction coefficient, and determines a toxicity index for a toxicity evaluation substance in the sample reaction chamber and the dilution reaction chamber. Effect of the Invention

[0025] The effects of the ecotoxicity monitoring device using the luminescent bacteria according to the present invention, which is adapted to rapidly and accurately monitor the presence or absence of toxicity in a sample, and the ecotoxicity monitoring method using the same, will be described as follows.

[0026] According to the present invention, the analysis unit calculates a correction coefficient based on the initial light intensity value and the final light intensity value measured from the reference reaction tank, and can quickly calculate the toxicity index for the toxicity evaluation substance in the sample reaction tank and the dilution reaction tank through the calculated correction coefficient. Since the ecotoxicity monitoring device using such luminescent bacteria can evaluate the presence or absence of a toxic substance in a sample within 5 minutes, it is possible to quickly respond to dangerous / harmful substances discharged into rivers or oceans.

[0027] The effects of the present invention are not limited to the effects described above, but should be understood to include all effects that can be inferred from the configuration of the invention described in the detailed description of the present invention or the claims. [Brief description of the drawings]

[0028] [Figure 1] 1 is a block diagram of an ecotoxicity monitoring device using luminescent bacteria according to an embodiment of the present invention; [Diagram 2] 1 is an exemplary diagram of an ecotoxicity monitoring device using luminescent bacteria according to an embodiment of the present invention; [Diagram 3] 1 is an exemplary diagram showing a process in which an initial light intensity value is measured after a mixture contained in a bacterial dilution chamber according to one embodiment of the present invention is supplied to a reference reaction chamber, a sample reaction chamber, and a dilution reaction chamber. [Figure 4] 1 is a schematic diagram illustrating a process in which a final light intensity value is measured after the required sample and dilution water are supplied to a reference reaction chamber, a sample reaction chamber, and a dilution reaction chamber according to an embodiment of the present invention. FIG. [Diagram 5] 1 is a flow chart showing a method for monitoring ecotoxicity using luminescent bacteria according to an embodiment of the present invention. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0029] The present invention will now be described with reference to the accompanying drawings. However, the present invention may be embodied in various different forms and is not limited to the embodiments described herein. In addition, in order to clearly illustrate the present invention in the drawings, parts that are not related to the description are omitted, and similar parts are designated by similar reference numerals throughout the specification.

[0030] Throughout the specification, when a part is described as being "connected" to another part, this includes not only the case where the part is "directly connected" to another part, but also the case where the part is "indirectly connected" to another part via another member in between. Furthermore, when a part is described as "comprising" a certain component, this does not mean that the part excludes other components, but that the part may further include other components, unless otherwise specified to the contrary.

[0031] In the present invention, the terms "upper" and "lower" refer to positions above or below a target member, and do not necessarily refer to positions above or below with respect to the direction of gravity.

[0032] Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

[0033] FIG. 1 is a configuration diagram of an eco-toxicity monitoring device using luminescent bacteria according to one embodiment of the present invention, FIG. 2 is an exemplary diagram of an eco-toxicity monitoring device using luminescent bacteria according to one embodiment of the present invention, FIG. 3 is an exemplary diagram showing an outline of a process in which an initial light intensity value is measured after a mixed liquid contained in a bacterial dilution tank according to one embodiment of the present invention is supplied to a reference reaction tank, a sample reaction tank and a dilution reaction tank, and FIG. 4 is an exemplary diagram showing an outline of a process in which a final light intensity value is measured after the required sample and dilution water are supplied to a reference reaction tank, a sample reaction tank and a dilution reaction tank according to one embodiment of the present invention.

[0034] As shown in Figures 1 to 4, an ecotoxicity monitoring device 1000 using luminescent bacteria may include a reaction tank module 100, a luminescent bacteria culture section 200, a dilution water supply section 300, a sample supply section 400, an optical measurement section 500, a control section 600 and an analysis section 700.

[0035] The ecotoxicity monitoring device 1000 may include, for example, a casing 810 for protecting various internal equipment from the outside.

[0036] The inside of the casing 810 may be partitioned, and for example, a monitor unit 820 that displays toxicity measurement results may be provided on the upper side, an analysis unit 700 may be provided below the monitor unit 820, an autosampler A may be provided below the analysis unit 700, and a reagent and waste liquid storage unit 830 may be stored below the autosampler A. The arrangement of each component provided within the casing 810 does not necessarily have to be configured in only the above-mentioned forms, and may be provided in various other forms.

[0037] Here, the autosampler A equipped with the reaction vessel module 100 and the optical measurement unit 500, etc., provided in the ecotoxicity monitoring device 1000, can be easily pulled out to the outside, for example, by a sliding method, or pulled into the inside of the casing 810 from the outside. With the autosampler A being able to slide in this way, if an abnormality occurs in any of the multiple components provided in the autosampler A, the user can easily repair or replace the component with the malfunction while the autosampler A itself is pulled out to the outside.

[0038] The ecotoxicity monitoring device 1000 is adapted to determine the presence or absence of a toxic substance in a sample using luminescent bacteria. That is, the ecotoxicity monitoring device 1000 measures the rate of change in the amount of luminescence of the luminescent bacteria due to the introduction of a sample, and determines the presence or absence of a toxic substance in the sample. Although the determination of the presence or absence of a toxic substance in a sample based on the rate of change in the amount of luminescence of the luminescent bacteria has already been disclosed in the prior art, the analysis unit 700 provided in the ecotoxicity monitoring device 1000 of the present invention calculates a correction coefficient based on the initial light amount value and the final light amount value measured from the reference reaction chamber 120, and more quickly determines the presence or absence of a toxic substance for the toxicity evaluation substances in the sample reaction chamber 130 and the dilution reaction chamber 140 based on the calculated correction coefficient.

[0039] Specifically, the reaction chamber module 100 may include a bacterial dilution chamber 110 , a reference reaction chamber 120 , a sample reaction chamber 130 and a dilution reaction chamber 140 .

[0040] Here, the bacteria dilution tank 110 is provided with a predetermined storage space, and a mixture containing luminescent bacteria and dilution water is stored inside the bacteria dilution tank 110. The luminescent bacteria are supplied to the bacteria dilution tank 110 from the luminescent bacteria culturing unit 200, and dilution water is supplied from the dilution water supply unit 300.

[0041] Here, luminescent bacteria that detect the presence or absence of toxicity of a sample are cultivated in the luminescent bacteria cultivation unit 200. The luminescent bacteria cultivation unit 200 is maintained at, for example, about 4° C. to suppress growth.

[0042] The dilution water supply unit 300 is adapted to store dilution water, for example, mineral water. The dilution water stored in the dilution water supply unit 300 does not necessarily have to consist of mineral water alone, and may store saline (NaCl) or the like.

[0043] The mixture containing the luminescent bacteria and dilution water supplied to the bacteria dilution tank 110 may be mixed uniformly through an agitator provided in the bacteria dilution tank 110 .

[0044] The bacteria dilution tank 110 is adapted to send a mixture of luminescent bacteria and dilution water in a predetermined ratio to the reference reaction tank 120, the sample reaction tank 130, and the dilution reaction tank 140, respectively. At this time, the mixture sent from the bacteria dilution tank 110 to each of the reference reaction tank 120, the sample reaction tank 130, and the dilution reaction tank 140 may be sent through, for example, a transfer pump (not shown). The movement of the mixture, as well as the sample and / or dilution water described below, may be performed by the operation of each transfer pump connected to the sample supply unit 400 and / or the dilution water supply unit 300, etc.

[0045] In addition, after a predetermined time (1 minute) has elapsed, the mixed liquid sent to the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 is subjected to measurement of the initial light intensity value of the luminescent bacteria in each reaction chamber through the optical measurement unit 500.

[0046] The optical measuring unit 500 includes an optical sensor unit 510 that measures the amount of minute luminescence of the luminescent bacteria that has flowed into the internal cell, and provides the measured initial light amount value to the analyzing unit 700 .

[0047] Here, the cell may be made of quartz material to optimally detect the amount of light. The cell may also be formed in a dark room to detect the emitted light. The optical sensor unit 510 may be, for example, a PMT (Photo Multiplier Tube). The optical sensor unit 510 measures the initial amount of light emitted by the luminescent bacteria when no sample is added and the final amount of light emitted by the luminescent bacteria after a predetermined time has elapsed with the sample added.

[0048] The number of the optical sensor units 510 may correspond to the number of the reaction chambers, that is, the number of the optical sensor units 510 may correspond to the number of the reference reaction chambers 120, the sample reaction chambers 130, and the dilution reaction chambers 140.

[0049] Here, the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 are in communication with the respective optical sensor units 510. Thus, a mixture of the luminescent bacteria contained in the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 may be supplied to each of the optical sensor units 510. Since each of the optical sensor units 510 is in communication with the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140, the mixture contained in the cell of the optical sensor unit 510 after the light quantity measurement is completed may be discharged to each of the reaction chambers.

[0050] The light intensity value of each reaction vessel measured in this manner by the optical sensor unit 510 is provided to the analysis unit 700.

[0051] After the light amount measurement for the mixed solution is completed, the cell in the light sensor unit 510 may be diluted with dilution water provided from the dilution water supply unit 300. That is, the light sensor unit 510 is configured to clean itself before a new light amount measurement. Thus, the light sensor unit 510 can measure an accurate light amount value for the provided evaluation material without being influenced by a previously used sample or luminescent bacteria.

[0052] In this manner, when the measurement of the initial light intensity values ​​for the mixed solutions provided from the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 is completed, a predetermined amount of dilution water is further supplied to the reference reaction chamber 120, a sample is supplied to the sample reaction chamber 130, and the sample and dilution water are supplied to the dilution reaction chamber 140 at a constant ratio. Here, the sample supply unit 400 supplies the sample only to the sample reaction chamber 130 and the dilution reaction chamber 140. The evaluation substances in the sample reaction chamber 130 and the dilution reaction chamber 140 to which the sample has been further supplied may be toxicity evaluation substances.

[0053] In this manner, after a certain period of time (5 minutes) has elapsed while further supplying predetermined amounts of sample and / or dilution water to the reference reaction chamber 120, sample reaction chamber 130, and dilution reaction chamber 140, the final light intensity values ​​are measured for the evaluation substance in the reference reaction chamber 120 to which only dilution water has been added, and for the toxicity evaluation substance in the sample reaction chamber 130 and dilution reaction chamber 140.

[0054] Thus, each final light quantity value measured from the optical sensor unit 510 is provided to the analysis unit 700. In addition, the evaluation substance / toxicity evaluation substance contained in the optical sensor unit 510 is discharged into the corresponding reaction tank, and the cell in the optical sensor unit 510 is washed with dilution water.

[0055] After the final light intensity value for each reaction chamber is measured, the evaluation substances / toxicity evaluation substances contained in the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 are discharged to the waste liquid storage unit 830 via a transfer pump. In addition, the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 are washed with dilution water in preparation for the next evaluation test.

[0056] Meanwhile, the control unit 600 controls the operation of various components provided in the ecotoxicity monitoring device 1000, such as the reaction bath module 100, the luminescent bacteria culturing unit 200, the dilution water supply unit 300, the sample supply unit 400, and the optical measurement unit 500. The control unit 600 controls various operations, such as supplying sample and / or dilution water to the reaction bath module 100 or cleaning the optical sensor unit 510.

[0057] Meanwhile, the analysis unit 700 is configured to calculate a toxicity index based on the initial light intensity values ​​and final light intensity values ​​measured from the evaluation substances / toxicity evaluation substances provided to the optical sensor unit 510 from the above-mentioned reference reaction chamber 120, sample reaction chamber 130 and dilution reaction chamber 140.

[0058] The analysis unit 700 is configured to measure the toxicity index (TI s ) is now required.

[0059] The toxicity index (TI) of such a sample reaction vessel s ) is calculated by the following calculation formula 1 to determine the correction coefficient of the standard reaction tank 120.

[0060]

number

[0061] [R0 = initial light intensity value after 1 minute of the mixture supplied from the bacteria dilution tank to the reference reaction tank, R1 = final light intensity value after 5 minutes when a fixed amount of dilution water is injected into the mixture in the reference reaction tank]

[0062] At this time, if the calculated correction coefficient is between 0.6 and 1.3, the analysis unit 700 judges it to be valid, and if the correction coefficient is outside the range of 0.6 to 1.3, the toxicity evaluation test is performed again with new mixed liquid supplied again from the bacteria dilution tank 110 to each reaction tank.

[0063] Furthermore, the analysis unit 700 obtains a corrected light intensity value of the sample reaction chamber 130 to which a correction coefficient is applied according to the following calculation formula 2. Here, the corrected light intensity value may be a light intensity value of the luminous bacteria to which a natural generation or reduction rate of the luminous bacteria is applied.

[0064]

number

[0065] [S0 = initial light intensity value after 1 minute of the mixture supplied from the bacteria dilution chamber to the sample reaction chamber, C0 = correction coefficient]

[0066] In addition, the analysis unit 700 calculates a toxicity index (TI) based on the final light intensity value of the sample reaction chamber 130 measured after a predetermined time (5 minutes) has elapsed after a predetermined required sample is injected into the sample reaction chamber 130 from the sample supply unit 400 according to the following formula 3: S ) is calculated.

[0067]

number

[0068] [S1 = corrected light intensity value after the first minute of the sample reaction chamber to which the correction coefficient has been applied, S2 = final light intensity value after a predetermined time has elapsed with a fixed amount of sample injected into the mixed solution in the sample reaction chamber]

[0069] In this way, when the toxicity index (TIs) of the sample reaction chamber 130 calculated by the analysis unit 700 is in the range of 20 to 80%, the analysis unit 700 determines that a toxic substance exists in the sample. The analysis unit 700 calculates a correction coefficient based on the initial light intensity value of the reference reaction chamber 120 and the final light intensity value measured after a predetermined time (5 minutes) has elapsed, and quickly determines whether or not a toxic substance exists in the sample finally accommodated in the sample reaction chamber 130 based on the calculated correction coefficient. In this way, the ecotoxicity monitoring device 1000 according to the present invention can determine whether or not a toxic substance exists in the sample more quickly than the conventional ecotoxicity monitoring device using luminescent bacteria.

[0070] Meanwhile, the analysis unit 700 measures the toxicity index (TI) of the dilution reaction tank 140 in the same manner as the sample reaction tank 130. d ) is required.

[0071] The toxicity index (TI) of such a dilution reactor 140 d ), the above-mentioned correction coefficient of the reference reaction tank 120 is used. Here, the details of the correction coefficient of the reference reaction tank 120 have been described above, so a detailed description thereof will be omitted.

[0072] Furthermore, the analysis unit 700 obtains a corrected light intensity value of the dilution reaction tank 140 to which a correction coefficient is applied according to the following calculation formula 4. Here, the corrected light intensity value may be a light intensity value of the luminous bacteria to which a natural generation or reduction rate of the luminous bacteria is applied.

[0073]

number

[0074] [D0 = initial light intensity value after 1 minute of the mixture supplied from the bacterial dilution tank to the dilution reaction tank, C0 = correction coefficient]

[0075] In addition, the analysis unit 700 calculates a toxicity index (TI) from the final light intensity value of the dilution reaction tank 140 measured after the sample and dilution water are injected into the dilution reaction tank 140 at a ratio required by the sample supply unit 400 and the dilution water supply unit 300 according to the following formula 5, and a predetermined time (5 minutes) has elapsed. d Here, the ratio of the sample and dilution water injected into the dilution reaction tank 140 may be 1:1.

[0076]

number

[0077] [D1 = corrected light intensity value after the first minute of the dilution reaction tank to which the correction coefficient has been applied, D2 = final light intensity value after a predetermined time has elapsed with a fixed amount of sample and dilution water injected into the mixture in the dilution reaction tank]

[0078] The toxicity index (TI) of the dilution reaction tank 140 calculated through the analysis unit 700 is d ) is used to evaluate the toxicity risk of the sample. For example, the toxicity index (TI S ) at 50% of the toxicity index (TI d ) should be around 25%, but the toxicity index (TI S ) at 50% of the toxicity index (TI d ) is 40%, it means that a large number of luminous bacteria were killed even with a small amount of sample, and the toxicity of the sample can be judged to be very high.

[0079] In this way, the analysis unit 700 calculates the toxicity index (TI S ) and the toxicity index (TI d ) and simultaneously evaluate the toxicity risk of the sample.

[0080] FIG. 5 is a flow chart showing a method for monitoring ecotoxicity using luminescent bacteria according to an embodiment of the present invention.

[0081] 5, the ecotoxicity monitoring method is first prepared by mixing luminescent bacteria and dilution water in the bacteria dilution tank 110. The luminescent bacteria are supplied to the bacteria dilution tank 110 from the luminescent bacteria culturing unit 200, and dilution water is supplied from the dilution water supply unit 300 (S100).

[0082] Next, the mixture in the bacteria dilution tank 110 is supplied to the reference reaction tank 120, the sample reaction tank 130, and the dilution reaction tank 140, respectively (S200).

[0083] Next, after a predetermined time has elapsed, the optical measurement unit 500 measures the initial light intensity values ​​of the luminescent bacteria in the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140. Here, the predetermined time during which the optical measurement unit 500 measures the initial light intensity values ​​of the luminescent bacteria in the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 may be one minute. The initial light intensity values ​​measured from each reaction chamber in this manner are provided to the analysis unit 700 (S300).

[0084] Next, after measuring the initial light intensity value, dilution water is supplied to the reference reaction chamber 120, a sample is supplied to the sample reaction chamber 130, and a predetermined amount of sample and dilution water is supplied to the dilution reaction chamber 140. The additional dilution water and sample supplied to the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140 may be supplied from the dilution water supply unit 300 and the sample supply unit 400 (S400).

[0085] Next, after a predetermined time has elapsed, the optical measurement unit 500 measures the final light intensity values ​​of the luminescent bacteria in the reference reaction chamber 120, the sample reaction chamber 130, and the dilution reaction chamber 140. Here, the predetermined time may be 5 minutes. In this manner, the final light intensity values ​​measured from each reaction chamber are provided to the analysis unit 700. (S500)

[0086] Finally, the analysis unit 700 calculates a toxicity index using the initial and final light intensity values ​​of each reaction vessel measured by the optical measurement unit 500, and determines whether or not the sample is toxic based on the calculated toxicity index (S600).

[0087] Here, the analysis unit 700 calculates a correction coefficient based on the initial light intensity value and final light intensity value of the reference reaction chamber 120 measured through the optical measurement unit 500, calculates corrected light intensity values ​​of the sample reaction chamber 130 and the dilution reaction chamber 140 based on the calculated correction coefficient, and finally obtains the toxicity index of the sample reaction chamber 130 and the dilution reaction chamber 140.

[0088] However, this is merely a preferred embodiment of the present invention, and the scope of the present invention is not limited to the description of this embodiment.

[0089] The above description of the present invention is for illustrative purposes only, and those skilled in the art will understand that the present invention can be easily modified into other specific forms without changing the technical idea or essential features of the present invention. Therefore, it should be understood that the above-described embodiments are illustrative in all respects and are not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, each component described as a distributed type may be implemented in a combined form.

[0090] The scope of the present invention is defined by the claims set forth below, and all modifications and variations that fall within the meaning and scope of the claims and their equivalent concepts should be construed as being included in the scope of the present invention. [Explanation of symbols]

[0091] 100 Reactor Module 110 Bacteria dilution tank 120 Reference Reactor 130 Sample Reaction Tank 140 Dilution Reactor 200 Luminous bacteria culture section 300 Dilution water supply section 400 Sample supply section 500 Optical measurement section 510 Optical sensor part 600 Control section 700 Analysis Department 810 Casing 820 Monitor section 830 Waste Liquid Storage Department 1000 Ecotoxicity Monitoring Device

Claims

1. a reaction chamber module including a reference reaction chamber, a sample reaction chamber, and a dilution reaction chamber, and a bacteria dilution chamber for supplying a mixture containing luminescent bacteria to the reference reaction chamber, the sample reaction chamber, and the dilution reaction chamber; a luminescent bacteria culturing unit for supplying luminescent bacteria for detecting the toxicity of a sample to the bacteria dilution tank; a dilution water supply unit for supplying dilution water to the bacteria dilution tank, the reference reaction tank, and the dilution reaction tank; a sample supply unit for supplying a sample to the sample reaction chamber and the dilution reaction chamber; an optical measurement unit for measuring the amount of luminescence of luminescent bacteria in a substance for toxicity evaluation provided from the reference reaction tank, the sample reaction tank and the dilution reaction tank to which a sample is supplied; a control unit for controlling the operation of the reaction vessel module, the luminescent bacteria culturing unit, the dilution water supply unit, the sample supply unit, and the optical measurement unit; an analysis unit that determines whether or not a toxic substance is present in a sample based on the light quantity value provided by the optical measurement unit; The analysis unit calculates a correction coefficient based on the initial light intensity value of the reference reaction chamber measured by the optical measurement unit and the final light intensity value measured after a predetermined time has elapsed, and calculates corrected light intensity values ​​of the sample reaction chamber and the dilution reaction chamber based on the calculated correction coefficient.

2. The analysis unit detects the toxicity index (TI) of the sample reaction vessel. ｓ ) but The toxicity index (TI ｓ )teeth, The correction coefficient of the reference reaction tank is calculated by the following calculation formula 1: A corrected light intensity value of the sample reaction vessel to which the correction coefficient is applied is calculated by the following calculation formula 2: A required sample is injected from the sample supply unit into the sample reaction chamber according to the following formula 3, and a toxicity index (TI) is calculated based on the final light intensity value of the sample reaction chamber measured after a predetermined time has elapsed. ｓ 2. The ecotoxicity monitoring device using the luminous bacteria according to claim 1, wherein the luminous bacteria is used to calculate the toxicity of the luminous bacteria. [0010] R0 = Initial light intensity value after 1 minute of the mixture supplied from the bacterial dilution chamber to the reference reaction chamber R1 = Final light intensity value after a predetermined time has elapsed with a fixed amount of dilution water injected into the mixture in the reference reaction tank [0025] S0 = initial light intensity value after 1 minute of the mixture supplied from the bacteria dilution tank to the sample reaction tank C0 = correction coefficient [0030] S1 = Corrected light intensity value after the first minute of the sample reaction vessel to which the correction coefficient is applied S2 = Final light intensity value after a predetermined time has elapsed with a fixed amount of sample injected into the mixed solution in the sample reaction tank

3. The analysis unit detects the toxicity index (TI) of the dilution reaction tank. ｄ ) but The toxicity index (TI ｄ )teeth, A corrected light quantity value of the dilution reaction vessel to which the correction coefficient of the reference reaction vessel is applied is calculated by the following calculation formula 4: According to the following formula 5, the sample and dilution water are injected into the dilution reaction tank at a required ratio from the sample supply unit and the dilution water supply unit, and a toxicity index (TI) is calculated based on the final light intensity value of the dilution reaction tank measured after a predetermined time has elapsed. ｄ 3. The ecotoxicity monitoring device using the luminous bacteria according to claim 2, wherein the luminous bacteria is used to calculate the toxicity of the luminous bacteria. [0045] D0 = initial light intensity value after 1 minute of the mixture supplied from the bacteria dilution tank to the dilution reaction tank C0 = correction coefficient [0050] D1 = Corrected light intensity value after the first minute of the dilution reaction vessel to which the correction coefficient is applied D2 = Final light intensity value after a predetermined time has elapsed with the sample and dilution water injected into the mixture in the dilution reaction tank

4. The analysis unit includes: If the calculated correction coefficient is between 0.6 and 1.3, it is determined to be valid; The calculated toxicity index (TI) of the sample reaction vessel ｓ 4. The ecotoxicity monitoring device using luminescent bacteria according to claim 3, wherein the presence of a toxic substance in the sample is determined when the concentration of the luminescent bacteria in the sample is 20-80%.

5. The analysis unit includes: The toxicity index (TI ｓ ) and the toxicity index (TI ｄ 5. The ecotoxicity monitoring device according to claim 4, wherein the toxicity of the sample is evaluated by comparing the amount of the luminescent bacteria.

6. 4. The ecotoxicity monitoring device using luminous bacteria according to claim 3, wherein the predetermined time for measuring the final light intensity values ​​of the reference reaction chamber, the sample reaction chamber and the dilution reaction chamber is 5 minutes.

7. (A) preparing a mixture of luminescent bacteria and dilution water in a bacteria dilution tank; (B) supplying the mixture from the bacteria dilution chamber to a reference reaction chamber, a sample reaction chamber and a dilution reaction chamber, respectively; (C) measuring the initial light quantity of the luminescent bacteria in the reference reaction chamber, the sample reaction chamber and the dilution reaction chamber through an optical measurement unit after a predetermined time has elapsed; (D) after measuring the initial light intensity value, supplying dilution water to the reference reaction chamber, supplying a sample to the sample reaction chamber, and supplying the sample and dilution water to the dilution reaction chamber in predetermined amounts; (E) measuring the final light quantity of the luminescent bacteria in the reference reaction chamber, the sample reaction chamber and the dilution reaction chamber through the optical measurement unit after a predetermined time has elapsed; (F) providing the initial light intensity value and the final light intensity value of each reaction vessel measured by the optical measurement unit to an analysis unit, and the analysis unit determines whether or not the sample is toxic; The analysis unit calculates a correction coefficient based on the initial light intensity value and the final light intensity value of the reference reaction tank measured by the optical measurement unit, calculates corrected light intensity values ​​of the sample reaction tank and the dilution reaction tank based on the calculated correction coefficient, and obtains toxicity indices for the toxicity evaluation substance in the sample reaction tank and the dilution reaction tank.

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