Production method for generating 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat

Abstract

To enable further extraction of DHMBA from raw oyster meat by extracting frozen raw oyster meat under pressure in an initial stage, and to enable a large quantity of highly concentrated DHMBA to be extracted in a short time.SOLUTION: The present invention is characterized by placing raw oysters in a container in which liquid for extraction is stored, sealing and pressurizing the container, concentrating the pressurized liquid for extraction, generating concentrated liquid containing highly concentrated 3,5-dihydroxy-4-methoxybenzyl alcohol, and generating 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to a production method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat.

Background Art

[0002] Conventionally, as a production method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol (hereinafter referred to as DHMBA) from raw oysters, raw oysters are heated at 1 atm for 1 hour, extracts are extracted from the raw oysters and sent to an extract, and then the boiled oysters are removed, and DHMBA is produced by heating or pressurizing the extract again.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] However, the inventor of the present invention was concerned that, for example, when raw oysters are heated and extracted at 1 atm for 1 hour, then the boiled oysters are removed, and DHMBA is produced by heating or pressurizing the extract again, the nutritional components in the oysters cannot be sufficiently extracted from the raw oysters. It was considered that a lot of nutritional components still remained in the removed boiled oysters.

[0005] That is, instead of heating raw oysters as in the prior art, performing the extraction operation, then taking out the raw oysters, reheating the remaining extract, and pressurizing to generate DHMBA from the extract, the present inventors hypothesized that more nutritional components could be extracted from the oysters by putting the raw oysters themselves, for example, frozen raw oysters themselves, into a liquid, heating them, and directly pressurizing them. In particular, it is speculated that a large amount of DHMBA can be extracted.

[0006] As described above, by putting the raw oysters themselves into a liquid, heating them, and directly performing pressure extraction, it is possible to obtain an oyster extract containing a large amount of nutritional components including DHMBA efficiently in a shorter time.

[0007] To explain the background of the present invention in more detail, when the present inventors started their business, they did not own an autoclave. Therefore, they heated frozen raw oyster meat at 1 atmosphere, then removed the boiled oyster meat, concentrated the extract of the remaining oyster meat, naturally cooled it to room temperature, and then added, for example, 99.9% ethanol to the concentrated oyster meat solution to perform natural precipitation, separating it into a supernatant and a precipitate. And then, they succeeded in creating the novel antioxidant DHMBA from the supernatant.

[0008] Next, the present inventors dedicated themselves to the technical development of increasing the DHMBA concentration in the supernatant obtained by heating at 1 atmosphere. And they invented that the DHMBA concentration can be increased by heating and pressurizing the extract obtained by heating oyster meat at 1 atmosphere, and obtained various patents.

[0009] However, in recent years, it has been speculated that DHMBA in frozen raw oyster meat may not be fully extracted only by heating at 1 atmosphere. Therefore, the present inventors hypothesized that more DHMBA can be extracted from raw oyster meat by performing pressure extraction on frozen raw oyster meat from the initial stage, and there is a possibility of extracting a high concentration of DHMBA in a short time and in a large quantity.

[0010] The inventor of the present invention already owns an autoclave at the research institute level and an autoclave at the factory level. Therefore, the pressurized heating extraction experiment of pressurized extraction of raw oyster meat from the initial stage could be smoothly carried out. When raw oyster meat is subjected to pressurized heating extraction from the initial stage, as shown in FIGS. 1 and 2, a high-concentration DHMBA-containing extract can be produced from the raw oyster meat, and moreover, a large amount of the extract can be produced in terms of quantity. Moreover, it was also confirmed that a high-concentration DHMBA-containing extract can be produced in a short time.

Means for Solving the Problems

[0011] The present invention involves putting raw oysters into a container in which an extraction liquid is stored, sealing and pressurizing the inside of the container, concentrating the pressurized extraction liquid, generating a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high concentration, and generating 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. It is characterized in that or putting raw oysters into a container in which an extraction liquid is stored, sealing and pressurizing the inside of the container, concentrating the pressurized extraction liquid, generating a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high recovery rate, and generating 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. It is characterized in that or Put raw frozen oysters into a container storing an extraction liquid approximately equal in weight to the raw oysters, seal and pressurize the inside of the container, concentrate the pressurized extraction liquid to produce a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high concentration, and produce 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. Characterized by Or Put raw frozen oysters into a container storing an extraction liquid approximately equal in weight to the raw oysters, seal and pressurize the inside of the container, concentrate the pressurized extraction liquid to produce a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high recovery rate, and produce 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. Characterized by Or The pressurization is to pressurize to 1 atm or more. Characterized by Or The extraction liquid is water. Characterized by Or The container is an autoclave. Characterized by the above.

Effect of the Invention

[0012] In the production method for generating 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat according to the present invention, by putting raw oysters themselves, for example, frozen raw oysters themselves into a liquid, heating them, and then pressurizing them as they are, a large amount of nutritional components can be extracted from the oysters. In particular, it is presumed that a large amount of DHMBA can be extracted.

[0013] And by putting raw oysters themselves into a liquid, heating them, and then performing pressure extraction as they are, there is an excellent effect that an oyster extract containing a large amount of nutritional components including a large amount of DHMBA can be efficiently obtained in a shorter time.

Brief Description of the Drawings

[0014]

Figure 1

Figure 2

Modes for Carrying Out the Invention

[0015] Hereinafter, regarding the present invention, an experiment was actually conducted to generate 3,5-dihydroxy-4-methoxybenzyl alcohol (DHMBA) using frozen raw oysters, and the experimental example will be described based on the drawings.

[0016] (Experimental Materials) As experimental materials, frozen oyster blocks produced in Hiroshima were used. That is, for the raw oysters used in the experiments for each condition setting, all were oysters thawed from the same frozen oyster blocks produced in Hiroshima. Also, in the experiments conducted by setting each condition, the usage amount of the frozen oyster blocks produced in Hiroshima used under one of the conditions was 1.5 kg.

[0017] As the liquid for heating the raw oysters, pure water was used, and in the experiment conducted by setting each condition, the amount of pure water used under one of the conditions was 1.5 kg. Therefore, the ratio of oysters to water was 1:1. Also, as the autoclave to be used, an autoclave (OAMVP - 005) manufactured by Kajiwara Co., Ltd. was used.

[0018] (Extraction conditions) First, the conditions in the extraction experiment conducted by setting each of the above - mentioned conditions are as follows. (1) Oysters:Water = 1:1, extraction at 94°C for 1 hour (1 atm · 1 hour) (2) Oysters:Water = 1:1, extraction at 135°C for 1 hour (3 atm · 1 hour) (3) Oysters:Water = 1:1, extraction at 94°C for 3 hours (1 atm · 3 hours) (4) Oysters:Water = 1:1, extraction at 135°C for 3 hours (3 atm · 3 hours)

[0019] And after extraction, vacuum concentration was performed to obtain a Brix of 40. Here, Brix represents the concentration of solid components dissolved in the liquid, and the concentrated liquid obtained by concentrating the oyster juice until the Brix reaches 40% is called a Brix 40 concentrated liquid. However, it is not limited to the measurement with the Brix 40 concentrated liquid obtained by concentrating the oyster juice until the Brix reaches 40%, and it may be measured with concentrated liquids concentrated to any %.

[0020] (Extraction method) From 10 kg of frozen oyster blocks, 6.0 kg of oysters (1.5 kg × 4 conditions) used in this experiment were thawed. After thawing, the weight of the oysters used under each of the above - mentioned conditions was measured and recorded. Then, the same amount of pure water as the weight of the oysters was prepared. For the extraction from oysters, the autoclave (OAMVP - 005) manufactured by Kajiwara Co., Ltd. was used as described above. 1.5 kg each of oysters and pure water in the same amount were put into the autoclave.

[0021] After charging, the lid of the kettle was closed, and under the extraction conditions of (1) and (3), heating was carried out until the temperature inside the kettle reached 94°C. When it reached 94°C, it was in a state of 1 atmosphere, and under the state of 1 atmosphere, heating extraction was carried out at 94°C for 1 hour in (1) and at 94°C for 3 hours in (3). In addition, the autoclave is provided with a pressure gauge, and a state of 1 atmosphere will be maintained when it reaches 94°C. Here, if the state of 1 atmosphere is maintained, the heating temperature is not limited to 94°C.

[0022] Under the extraction conditions of (2) and (4), the lid of the kettle and the pressure valve were closed, and pressurization was carried out until the temperature inside the kettle reached 135°C. When it reached 135°C, the pressure gauge maintained 3 atmospheres. And under the state of 3 atmospheres, pressurization extraction was carried out at 135°C for 1 hour in (2) and at 135°C for 3 hours in (4). In addition, a state of 3 atmospheres will be maintained when it reaches 135°C, but if the state of 3 atmospheres is maintained, the heating temperature is not limited to 135°C.

[0023] After the extraction was completed, the Brix value, weight of the extract (boiled juice) obtained under each condition, and the weight of the boiled oyster residue after extraction were measured. The boiled juice under each condition was concentrated using a rotary evaporator until the Brix value of the boiled juice reached 40. At this time, the temperature of the water bath of the rotary evaporator was set to 95°C. After the concentration was completed, the weight of the Brix 40 extract obtained from the boiled juice under each condition was measured. The measured weight of each Brix 40 extract was divided by the weight of the oysters used for extraction to calculate the recovery rate (yield) of the Brix 40 extract weight from the oyster weight.

[0024] In addition, the DHMBA concentration in each Brix 40 extract was quantified using a liquid chromatograph / tandem mass spectrometer (LC-MS / MS: Prominence high-pressure gradient HPLC, triple quadrupole mass spectrometer LCMS-8040 system, Shimadzu Corporation). The weights of the oysters and pure water used under each extraction condition, the required time for heating and pressurization, and the numerical values related to concentration are shown in Table 1 of Figure 1.

[0025] (Measurement items) The items measured this time are as follows. 1 Recovery rate of Brix 40 extract weight from raw oyster weight This is because if the recovery rate of the Brix 40 extract weight is high, it is predicted that the recovery rate of DHMBA will be high. 2 DHMBA concentration in the Brix 40 extract This is because if the DHMBA concentration in the Brix 40 extract is high, it is predicted that the amount of DHMBA will be large. However, the above measurement results are shown in Figure 2.

[0026] (Conclusion) As shown by the above results, the weight of the Brix 40 extract obtained by pressurized extraction at 3 atmospheres was greater than that by extraction at 1 atmosphere. This indicates that more nutritional components in oysters could be extracted by pressurized extraction at 3 atmospheres compared to extraction at 1 atmosphere. Also, the DHMBA concentration was higher with pressurized extraction at 3 atmospheres.

[0027] In the extraction methods for which the inventor of this case has obtained patents, for example, after extracting nutritional components from oysters in a 1 - atmosphere state, the oysters are removed and only the extract is heated or pressurized to generate DHMBA. In this extraction method, the oysters are removed before pressurization, and the extraction time of oysters is short, for example, 1 hour. Therefore, it is considered that many important nutritional components including DHMBA remain in the oysters.

[0028] As shown in Figure 2, in the new pressurized extraction method, it was confirmed that the extraction amount and generation amount of DHMBA from raw oysters were increased at the initial stage, that is, by directly pressurizing the raw oysters, compared to the previously patented method. Furthermore, the method of directly putting oysters into pure water, heating, and pressurizing for extraction can perform the extraction operation in a much shorter time than the method described in the existing patents obtained by the inventor of this case.

[0029] As described above, it can be said that the extraction method of the present invention, which directly pressurizes raw oysters from the initial stage of extraction from raw oysters, is an excellent extraction liquid production method and DHMBA production method from raw oysters as compared with the conventional extraction method. That is, the inventive method of directly pressurizing and extracting raw oysters can efficiently obtain a large amount of DHMBA in a short time compared with existing patents.

Claims

**Claim 1** Put fresh oysters into a container in which an extraction liquid is stored, seal and pressurize the inside of the container, concentrate the pressurized extraction liquid, and generate a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high concentration, and generate 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. A production method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat, characterized by the above. **Claim 2** Put fresh oysters into a container in which an extraction liquid is stored, seal and pressurize the inside of the container, concentrate the pressurized extraction liquid, and generate a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high recovery rate, and generate 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. A production method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat, characterized by the above. **Claim 3** Put frozen fresh oysters into a container in which an extraction liquid approximately equal in weight to the fresh oysters is stored, seal and pressurize the inside of the container, concentrate the pressurized extraction liquid, and generate a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high concentration, and generate 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. A production method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat, characterized by the above. **Claim 4** Put raw oysters into a container in which an extraction liquid approximately equal in weight to the raw oysters is stored, seal and pressurize the inside of the container, concentrate the pressurized extraction liquid to produce a concentrated liquid containing 3,5-dihydroxy-4-methoxybenzyl alcohol with a high recovery rate, and produce 3,5-dihydroxy-4-methoxybenzyl alcohol from the concentrated liquid. A manufacturing method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat, characterized by the above.

5. The pressurization is to pressurize to 1 atm or more. A manufacturing method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat according to Claim 1 or Claim 2, characterized by the above.

6. The extraction liquid is water. A manufacturing method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat according to Claim 1 or Claim 2, characterized by the above.

7. The container is an autoclave. A manufacturing method for producing 3,5-dihydroxy-4-methoxybenzyl alcohol from oyster meat according to Claim 1 or Claim 2, characterized by the above.

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