Cosmetic composition, use of penetration enhancer, and manufacturing method

Abstract

To provide a novel penetration enhancer for royal jelly into the skin.SOLUTION: The present invention provides a cosmetic composition comprising 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid, for promoting penetration of 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid into the skin, the cosmetic composition including carrageenan as an active ingredient.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a penetration enhancer of royal jelly into the skin.

Background Art

[0002] Royal jelly is known to exhibit beauty effects such as preventing skin aging and leading to moist and firm skin when applied to the skin (for example, Patent Document 1). These beauty effects are considered to be due to fatty acids peculiar to royal jelly such as 10-hydroxy-2-decenoic acid. Therefore, in order to fully exhibit the beauty effects of royal jelly, the permeability of royal jelly, particularly the fatty acids peculiar to royal jelly, into the skin is important. Patent Document 2 discloses a topical skin preparation containing royal jelly extract as a skin activating component, and it is described that the topical skin preparation enhances the permeability of skin active components into the skin by stably retaining moisture on the skin for a long time.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Patent Document 2

Patent Document 3

Summary of the Invention

Problems to be Solved by the Invention

[0004] An object of the present invention is to provide a new penetration enhancer of royal jelly into the skin.

Means for Solving the Problems

[0005] In previous studies by the present inventors, it was found that (acrylates / alkyl acrylates (C10-30)) cross-polymer and (ammonium acryloyldimethyltaurate / beheneth-25 methacrylate) cross-polymer have the effect of promoting the penetration of royal jelly into the skin (Patent Document 3). As a result of further research to achieve the above object, the present inventors newly found that carrageenan used as a cosmetic additive promotes the penetration of royal jelly into the skin, leading to the completion of the present invention.

[0006] That is, the present invention relates to, for example, the following inventions. [1] A penetration enhancer for royal jelly into the skin, containing carrageenan as an active ingredient. [2] The penetration enhancer according to [1], wherein the above royal jelly contains saturated fatty acids and / or unsaturated fatty acids. [3] The penetration enhancer according to [2], wherein the above saturated fatty acid contains 10-hydroxydecanoic acid. [4] The penetration enhancer according to [2], wherein the above unsaturated fatty acid contains at least one selected from the group consisting of 10-hydroxy-2-decenoic acid, decenedioic acid, and sebacic acid. [5] A cosmetic composition containing the penetration enhancer according to any one of [1] to [4] and royal jelly. [6] The above royal jelly is a filtrate obtained by filtering a solution of enzymatically treated royal jelly through a filter with a pore size of 3 μm, and the above cosmetic composition contains butylene glycol, pentylene glycol, and phenoxyethanol. The cosmetic composition according to [5]. [7] A method for promoting the penetration of royal jelly into the skin, including blending the penetration enhancer according to any one of [1] to [4] into a cosmetic composition containing royal jelly. [8] A method for promoting the penetration of royal jelly into the skin, including applying the cosmetic composition according to [5] or [6] to the skin. [9] A method for producing a cosmetic composition containing royal jelly, including blending the penetration enhancer according to any one of [1] to [4] and royal jelly into the cosmetic composition.

[10] The royal jelly is a filtrate obtained by filtering a solution of enzymatically treated royal jelly through a filter with a pore size of 3 μm, and the cosmetic composition contains butylene glycol, pentylene glycol, and phenoxyethanol, which is the production method of [9].

Advantages of the Invention

[0007] According to the penetration enhancer of the present invention, by promoting the penetration of royal jelly into the skin, the cosmetic effect of the cosmetic composition containing royal jelly can be enhanced.

Brief Description of the Drawings

[0008]

Figure 1

Figure 2

Embodiments for Carrying Out the Invention

[0009] Hereinafter, the embodiments for carrying out the present invention will be described in detail. However, the present invention is not limited to the following embodiments.

[0010] The penetration enhancer for royal jelly into the skin of the present invention contains carrageenan as an active ingredient.

[0011] Carrageenan is a carbohydrate obtained from red algae such as the Chondrus family and the Gigartinaceae family. Specifically, it is a linear sulfated polysaccharide composed of repeating units of galactose and 3,6-anhydrogalactose linked by alternating bonds of α1-3 and β1-4 glycosides. It is known that there are three main types of carrageenan: lambda carrageenan, kappa carrageenan, and iota carrageenan. The carrageenan in the present invention may be any of lambda carrageenan, kappa carrageenan, and iota carrageenan, and any combination thereof.

[0012] As the carrageenan, those obtained by extracting from red algae or the like by a conventionally known method (for example, the method described in JP-A-2009-1700) and optionally purified may be used, or commercially available products may be used. Examples of commercially available carrageenans include purified carrageenans such as GENUGEL (trademark) carrageenan Type CJ; Sankyo Co., Ltd.). The purified carrageenan may contain other components such as additives in addition to carrageenan, and may contain, for example, sucrose. The mass ratio of carrageenan: sucrose is not particularly limited, and may be, for example, 1 to 10: 1 to 10, and may be, for example, 2: 1, 7: 3, 6: 4, 1: 1, etc.

[0013] Carrageenan is a cosmetic ingredient known as a thickening agent and a gelling agent, but the effect of promoting the penetration of royal jelly into the skin has not been known so far.

[0014] Promotion of the penetration of royal jelly into the skin means that the components contained in royal jelly, particularly the active ingredients having a beauty effect, such as saturated fatty acids and unsaturated fatty acids, penetrate deeper into the stratum corneum of the skin than when no penetration enhancer is contained. Here, examples of the saturated fatty acid include 10-hydroxydecanoic acid, 8-hydroxyoctanoic acid, 12-hydroxydodecanoic acid, 3,10-dihydroxydecanoic acid, capric acid, etc., and examples of the unsaturated fatty acid include 10-hydroxy-2-decenoic acid, decenedioic acid, and sebacic acid, trans-2-decenoic acid, etc. Preferably, the saturated fatty acid is 10-hydroxydecanoic acid, and the unsaturated fatty acids are 10-hydroxy-2-decenoic acid, decenedioic acid, and sebacic acid.

[0015] The promotion of the penetration of royal jelly into the skin can be evaluated by analyzing the components contained in royal jelly, particularly components such as saturated fatty acids and unsaturated fatty acids, by the method shown in the examples, for example. Specifically, a composition containing royal jelly and a penetration enhancer is applied to the skin, and after a predetermined time (for example, 30 minutes later, 1 hour later, 2 hours later), a stratum corneum sample is collected by a tape stripping test, and a method of detecting royal jelly adhering to the tape can be mentioned.

[0016] The method for detecting the royal jelly components that have penetrated into the stratum corneum is not particularly limited. For example, it may be detected by imaging using a mass spectrometer as described in the examples of the present application.

[0017] (Royal jelly) Royal jelly is a milky jelly-like substance made by mixing the secretions secreted from the hypopharyngeal gland and the major parotid gland of worker bees aged 3 to 12 days among honeybees. The main bioactive components in royal jelly include, for example, saturated fatty acids peculiar to royal jelly, and organic acids such as unsaturated fatty acids, as well as proteins, amino acids, peptides, lipids, saccharides, vitamin Bs, folic acid, nicotinic acid, pantothenic acid and other vitamins, various minerals, etc.

[0018] The royal jelly in the present invention includes raw royal jelly, dried royal jelly, dried royal jelly powder, enzyme-treated royal jelly, royal jelly extract, royal jelly fermented product, etc. Also, the origin of the royal jelly may be any of European countries, Oceanian countries, the United States, Brazil, Japan, China, and other Asian countries, etc.

[0019] Dried royal jelly powder is obtained by drying raw royal jelly and pulverizing it. As the drying method, any known method employed in general food processing, such as natural drying such as ventilation drying and sun drying, forced drying by heating with electricity, etc., and freeze drying can be used. Preferably, it is freeze drying.

[0020] Enzyme-treated royal jelly is obtained by treating royal jelly with a proteolytic enzyme (protease). Preferably, it is a low-allergen enzyme-treated royal jelly in which the allergic reaction caused by the protein contained in royal jelly is suppressed by protease treatment. Therefore, enzyme-treated royal jelly may contain, in addition to the protease degradation products of the proteins contained in royal jelly, organic acids such as the aforementioned saturated fatty acids and unsaturated fatty acids, lipids, saccharides, vitamins, and various minerals.

[0021] The royal jelly used in the production of enzyme-treated royal jelly is not particularly limited, and examples include raw royal jelly, royal jelly powder obtained by drying raw royal jelly into powder, or an extract obtained by extracting raw royal jelly with water or hydrous ethanol, etc.

[0022] The production of enzyme-treated royal jelly can be carried out by treating a royal jelly raw material with an enzyme having at least endopeptidase activity, an enzyme having at least exopeptidase activity, and / or an enzyme having both endopeptidase activity and exopeptidase activity.

[0023] Examples of proteolytic enzymes having at least endopeptidase activity include endopeptidases derived from animals (e.g., trypsin, chymotrypsin, etc.), plants (e.g., papain, etc.), and microorganisms (e.g., lactic acid bacteria, yeast, mold, Bacillus subtilis, actinomycetes, etc.).

[0024] Examples of proteolytic enzymes having at least exopeptidase activity include carboxypeptidase, aminopeptidase, exopeptidases derived from microorganisms (e.g., lactic acid bacteria, Aspergillus spp., Rhizopus spp., etc.), pancreatin having endopeptidase activity as well, and pepsin, etc.

[0025] Among such various enzymes, preferred examples of enzymes having both exopeptidase activity and endopeptidase activity include peptidase produced by Streptomyces griseus (trade name: Actinase AS), peptidase produced by Aspergillus oryzae (trade names: Protease A, Flavorzyme, Proteax), peptidase produced by Aspergillus melleus (trade name: Protease P). Also, preferred examples of enzymes having exoprotease activity include peptidase produced by Aspergillus oryzae (trade names: Umamizyme G, Promod 192P, Promod 194P, Sumitomo FLAP), peptidase produced by Aspergillus sojae (trade name: Sternzyme B15024), peptidase produced by Aspergillus genus (trade name: Kokurase P), peptidase produced by Rhizopus oryzae (trade name: Peptidase R). Further, preferred examples of enzymes having endoprotease activity include peptidase produced by Bacillus subtilis (trade names: Orientase 22BF, Nucleicin), peptidase produced by Bacillus licheniformis (trade name: Alcalase), peptidase produced by Bacillus stearothermophilus (trade name: Protease S), peptidase produced by Bacillus amyloliquefaciens (trade name: Neutrase), peptidase produced by Bacillus genus (trade name: Protamex).

[0026] Enzymatic treatment for reducing the allergy of royal jelly can be carried out, for example, according to the descriptions in JP-A-2007-295919 and JP-A-2007-295920.

[0027] The royal jelly extract is obtained by extracting royal jelly (including raw, dried, and pulverized forms) with water, hydrous ethanol, or the like.

[0028] The royal jelly fermented product can be produced by a conventional method using microorganisms such as yeast and lactic acid bacteria.

[0029] Commercially available royal jelly may be used. Specific examples of royal jelly include, for example, royal jelly FD powder (Nakamoto Co., Ltd.), royal jelly extract SF (Matsura Pharmaceutical Co., Ltd.), deproteinized royal jelly powder F (Maruzen Pharmaceutical Co., Ltd.), deproteinized royal jelly extract (Api Co., Ltd.), and the like.

[0030] Royal jelly is not particularly limited as long as it has, for example, a beauty effect such as preventing skin aging and leading to moist and firm skin. However, it preferably contains saturated fatty acids and / or unsaturated fatty acids. It preferably contains 10-hydroxy-2-decenoic acid as the saturated fatty acid, and preferably contains at least one selected from the group consisting of 10-hydroxydecanoic acid, decenedioic acid, and sebacic acid as the unsaturated fatty acid. The penetration enhancer of the present invention can be used in skin external preparations, cosmetic compositions, and the like containing royal jelly.

[0031] (Cosmetic composition) The cosmetic composition of the present invention contains the penetration enhancer for royal jelly of the present invention to the skin and royal jelly.

[0032] The content of carrageenan in the cosmetic composition may be an amount (effective amount) capable of exerting the effect of promoting the penetration of royal jelly into the skin. For example, it may be 0.01% by mass or more, 0.1% by mass or more, 0.3% by mass or more, 0.4% by mass or more, 0.5% by mass or more, 1% by mass or more, 2% by mass or more, 3% by mass or more, 5% by mass or more, based on the total amount of the cosmetic composition, and may be 20% by mass or less, 15% by mass or less, 10% by mass or less, 8% by mass or less, 5% by mass or less, 3% by mass or less, 2% by mass or less, 1% by mass or less, 0.5% by mass or less.

[0033] The content of royal jelly in the cosmetic composition is not particularly limited as long as the royal jelly can exhibit its beauty effects. For example, it may be 0.1% by mass or more, 0.5% by mass or more, 1% by mass or more, 1.5% by mass or more, 2% by mass or more, 3% by mass or more, 5% by mass or more, 8% by mass or more, 10% by mass or more, 15% by mass or more, based on the total amount of the cosmetic composition, and may be 20% by mass or less, 15% by mass or less, 10% by mass or less, 8% by mass or less, 5% by mass or less, 3% by mass or less, 2% by mass or less, 1% by mass or less, 0.5% by mass or less.

[0034] The cosmetic composition may be in any form such as solid, liquid, paste, etc. The cosmetic may be a medicinal cosmetic (i.e., quasi-drug). The cosmetic composition includes all cosmetic compositions that can be applied to parts such as the skin, mucous membranes, body hair, hair, scalp, nails, teeth, facial skin, and lips of animals (especially humans). Among these, from the perspective of promoting the penetration of royal jelly, it is preferably a cosmetic composition applied to the whole body skin, such as the face, scalp, lips, etc.

[0035] In addition to the penetration enhancer and royal jelly of the present invention, the cosmetic composition may contain components acceptable as cosmetics. Examples of components acceptable as cosmetics include whitening agents, moisturizing agents, antioxidants, oily components, ultraviolet absorbers, surfactants, thickeners, alcohols, powder components, coloring materials, aqueous components, water, and various skin nutrients.

[0036] The dosage form of the cosmetic composition may be, for example, a solubilization system, an emulsification system, a powder system, an oil-liquid system, a gel system, an ointment system, an aerosol system, a water-oil two-layer system, a water-oil-powder three-layer system, etc. Examples of cosmetics include basic cosmetics such as facial cleansers, lotions, milks, creams, gels, essences, beauty liquids, packs, masks, mists, UV protection cosmetics, etc., makeup cosmetics such as foundations, lipsticks, blushes, eyeshadows, eyeliners, mascaras, etc., facial cleansers, massage agents, cleansing agents, aftershave lotions, pre-shave lotions, shaving creams, body soaps, soaps, shampoos, rinses, hair treatments, hair styling agents, hair tonics, hair mists, hair foams, hair liquids, hair gels, hair sprays, hair growth agents, antiperspirants, bath salts, mouth rinses, oral cosmetics, toothpastes, hand creams, hand soaps, etc.

[0037] The production method of the above cosmetic composition is not particularly limited and can be appropriately carried out according to known methods. For example, the penetration enhancer and royal jelly can be mixed with an intermediate product or a final product in the production process of cosmetics to obtain the cosmetic composition used for the above purposes.

[0038] (Method for promoting the penetration of royal jelly into the skin) A method for promoting the penetration of royal jelly into the skin according to one embodiment includes blending the penetration enhancer of the present invention into a cosmetic composition containing royal jelly. By blending the penetration enhancer of the present invention into a cosmetic composition containing royal jelly, when the obtained cosmetic composition is applied to the skin, due to the presence of the penetration enhancer of the present invention, the penetration of royal jelly contained in the cosmetic composition into the skin is promoted more than that of a cosmetic composition not containing the penetration enhancer of the present invention.

[0039] A method for promoting the penetration of royal jelly into the skin according to another embodiment includes applying the cosmetic composition of the present invention to the skin. By applying the cosmetic composition of the present invention to the skin, due to the presence of the penetration enhancer of the present invention, the penetration of royal jelly contained in the cosmetic composition into the skin is promoted more than that of a cosmetic composition not containing the penetration enhancer of the present invention.

Example

[0040] The following examples are shown to specifically describe the present application. Note that the present application is not limited to the following examples.

[0041] (Preparation of Enzyme-Treated Royal Jelly Extract) The enzyme-treated royal jelly extract was prepared from raw royal jelly according to the method described in WO2020 / 196065 A1. Specifically, 400 mL of ion-exchanged water was added to 474 g of raw royal jelly and stirred until uniform to prepare a royal jelly dilution. 2N NaOH aqueous solution was added to adjust the pH of the royal jelly dilution to 7.8. Next, a solution prepared by dissolving 4.75 g of Proteax (Amano Enzyme Inc.), which has both endopeptidase action and exopeptidase action, in 20 mL of ion-exchanged water was added to the royal jelly dilution, and further ion-exchanged water was added until the total volume reached 980 g. The reaction mixture was reacted at 50 °C (constant temperature water bath) for 2 hours while stirring with a propeller to perform hydrolysis. The temperature of the constant temperature water bath was raised to 80 °C and stirred for 15 minutes to inactivate the protease. The obtained enzyme-treated royal jelly solution was filtered through a filter with a pore size of 3 μm to obtain an enzyme-treated royal jelly solution. Butylene glycol, pentylene glycol, phenoxyethanol, and purified water were added so that the concentration of the enzyme-treated royal jelly solution became 2.5% by weight to obtain an enzyme-treated royal jelly extract.

[0042] (Preparation of Cosmetic Composition of Example) As a cosmetic composition of the example, a cosmetic composition containing royal jelly and carrageenan was prepared.

[0043] The enzyme-treated royal jelly extract, GENUGEL (trademark) carrageenan type CJ as a penetration enhancer, and other raw materials listed in Table 1 were mixed in a predetermined formulation to prepare a cosmetic composition of the example. The composition of the obtained cosmetic composition is shown in Table 1. Note that sucrose is an additive contained in GENUGEL (trademark) carrageenan type CJ (carrageenan: sucrose = 7:3).

[0044]

Table 1

[0045] (Preparation of Cosmetic Composition of Comparative Example) The enzyme-treated royal jelly extract and other raw materials listed in Table 2 were mixed in a predetermined formulation to prepare a cosmetic composition of the comparative example. The composition of the obtained cosmetic composition of the comparative example is shown in Table 2.

[0046]

Table 2

[0047] (Skin Penetration Test) The skin permeability of the cosmetic compositions of the examples and comparative examples was evaluated by the following method. First, samples were prepared by tape stripping test. The forearm of one volunteer was washed and left at room temperature for 15 minutes. The cosmetic compositions of the examples and comparative examples were applied to a 6.25 cm 2 square skin on the forearm in an amount of 30 mg / cm 2 and allowed to stand for 2 hours. The application area was washed and left for 15 minutes. Then, a circular tape (D-Squame sampling discs (CUDERM)) with a diameter of 2 cm was applied to the applied skin area, pressurized for 1 minute, and then the circular tape was peeled off. The central part of the circle of the peeled circular tape was cut with a width of 5 mm to obtain an imaging mass spectrometry (imaging MS) sample. Similarly, the circular tape was applied to the skin area and peeled off 9 more times, and a total of 10 imaging MS samples were obtained by cutting the central part of the circle of the circular tape with a width of 5 mm. The skin before applying the test cosmetic composition was also used as a control to prepare 10 samples in the same way.

[0048] Imaging MS was performed using a DESI Xevo G2 XS QTof (Waters). The amounts of 10-hydroxy-2-decenoic acid and 10-hydroxydecanoic acid contained in royal jelly were quantified in each sample by detecting ions with m / z 185.1172 and m / z 187.1328.

[0049] Figures 1 and 2 show the results of imaging MS indicating the skin permeability of 10-hydroxy-2-decenoic acid and 10-hydroxydecanoic acid, respectively. From Figures 1 and 2, in the cosmetic compositions containing sodium alginate of the control and comparative examples, neither 10-hydroxy-2-decenoic acid nor 10-hydroxydecanoic acid was detected in any of the tapes, whereas in the cosmetic composition containing carrageenan of the example, 10-hydroxy-2-decenoic acid and 10-hydroxydecanoic acid were detected up to the fourth tape. From this, it was found that the addition of carrageenan can promote the penetration of royal jelly into the skin.

Claims

1. A cosmetic composition containing 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid for promoting the penetration of 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid into the skin, comprising carrageenan as an active ingredient.

2. 2. The cosmetic composition according to claim 1, wherein 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid is contained as royal jelly.

3. Use of a skin permeation enhancer for 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid, which comprises carrageenan as an active ingredient, in a cosmetic composition containing 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid.

4. The use according to claim 3, wherein 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid are contained in the cosmetic composition as royal jelly.

5. The use according to claim 4, wherein the royal jelly is a filtrate obtained by filtering a solution of enzyme-treated royal jelly through a particle pore size of 3 μm, and the cosmetic composition contains butylene glycol, pentylene glycol, and phenoxyethanol.

6. A method for producing a cosmetic composition containing 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid for promoting the penetration of 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid into the skin, comprising: A method of manufacturing the cosmetic composition comprising incorporating carrageenan into the cosmetic composition.

7. The method according to claim 6, wherein 10-hydroxydecanoic acid and / or 10-hydroxy-2-decenoic acid is contained in the cosmetic composition as royal jelly.

8. The method according to claim 7, wherein the royal jelly is a filtrate obtained by filtering a solution of enzyme-treated royal jelly through a filter with a particle pore size of 3 μm, and the cosmetic composition contains butylene glycol, pentylene glycol, and phenoxyethanol.

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