Microchannel device, electrode substrate thereof, and method for using microchannel device

Abstract

To provide a microchannel device which is easy to manufacture and can obtain accurate outputs, and some components of which are repeatedly usable.SOLUTION: A microchannel device 1 comprises: a channel body 11 in which are formed a channel 13 composed of a narrow groove in a substrate 12, a sample introduction unit 15c for introducing a liquid sample for analysis into the channel 13, and a sample collection unit 15b for collecting the liquid sample after being introduced into the channel 13 from the sample introduction unit 15c and having undergone capillary electric induction; a cover member 31 stacked on top of the channel body 11 so as to cover the channel 13 of the channel body 11; an electrode substrate 51 in which are formed electrode patterns 53, 55 disposed separately from the cover member 31 on the plane of the cover member 31 that is opposite the channel body 11 and having detection parts 53a, 55a for liquid sample analysis by capillary electric induction at a position facing the channel 13 of the channel body 11; and fixation means 71 for fixing the electrode substrate 51 in place on the cover member 31.SELECTED DRAWING: Figure 3

Description

Technical Field

[0001] The present invention relates to a microchannel device used for synthesis, separation, analysis, etc. of liquid samples, an electrode substrate thereof, and a method of using the microchannel device.

Background Art

[0002] Conventionally, chromatography is generally used as a method for separating and analyzing a substance to be detected contained in a sample. Similarly, ion chromatography is used when separation and analysis are performed based on electrical properties. When these samples are separated and analyzed using a liquid moving layer, it is called liquid chromatography LC or high performance liquid chromatography HPLC.

[0003] On the other hand, in recent years, for charged substances (ions) such as inorganic ions, organic acids, amino acids, and biomolecules, analysis by capillary electrophoresis has attracted attention more than this liquid ion chromatography LC (HPLC).

[0004] Analysis of a liquid sample by capillary electrophoresis is performed using a microchannel. A microchannel injects a buffer solution and a liquid sample to be analyzed into a fine channel, and measures components of the liquid sample, etc. by electrophoresis occurring in the channel. Compared with liquid chromatography LC (HPLC), (1) A smaller amount of sample (2) Miniaturization of the analytical instrument (3) Analysis at high speed and in a short time (4) High resolution has significance.

[0005] By the way, a microchannel device having a microchannel for measurement by capillary electrophoresis requires an electrode for electrical measurement.

[0006] For example, Patent Document 1 discloses a microchannel device (1) in which a substrate (10) is constituted by a main substrate (11) having a groove serving as a flow path (13) and a cover member (31) covering the flow path (13) of the main substrate, and electrodes (33, 35) are formed on the surface of the cover member (31) of the substrate (10) on the side not facing the flow path (13). According to this microchannel device (1), since the electrodes (33, 35) are formed on the surface of the cover member (31) on the side not facing the flow path (13), the electrodes (33, 35) for analyzing a liquid sample by electrophoresis can be easily and surely arranged in the vicinity of the flow path (13) in a non-contact manner with the liquid sample, and highly accurate detection can be performed.

Prior Art Documents

Patent Documents

[0007]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0008] However, in the case of the microchannel device (1) shown in the above Patent Document 1, since the cover member (31) is laminated on the upper surface of the main substrate (11) and then the electrodes (33, 35) are formed on the upper surface of the cover member (31), it is not always easy to perform accurate alignment with the flow path (13) when forming the electrodes (33, 35). Although a method of forming the electrodes (33, 35) in advance when the cover member (31) is alone and then attaching this cover member (31) onto the main substrate (11) is conceivable, in that case, while attaching and fixing the cover member (31) onto the main substrate (11), at the same time, alignment of the flow path (13) and the electrodes (33, 35) must be performed, and that operation is not always easy.

[0009] In addition, since the microchannel device (1) has a structure that is discarded once used (so-called disposable structure), the parts cannot be reused, resulting in waste.

[0010] The present invention has been made in view of the above points, and its object is to provide a microchannel device that can be easily manufactured to obtain accurate output, can repeatedly use some components, and can reduce waste of the components, an electrode substrate thereof, and a method of using the microchannel device.

Means for Solving the Problems

[0011] The present invention includes a channel body formed in a substrate with a channel composed of narrow grooves, a sample introduction part for introducing a liquid sample for analysis into the channel, and a sample recovery part for recovering the liquid sample after being introduced into the channel from the sample introduction part and being subjected to capillary electrophoresis induction, a cover member laminated on the channel body so as to cover the channel of the channel body, and an electrode pattern having a detection part for liquid sample analysis by capillary electrophoresis induction is formed on a surface of the cover member opposite to the channel body and placed separately from the cover member at a position facing the channel of the channel substrate, and an electrode substrate. The microchannel device is characterized by comprising the above. According to the present invention, since the electrode pattern such as the detection part is provided on an electrode substrate separate from the cover member and this electrode substrate is placed on the cover member, the detection part can be accurately opposed and installed in the channel. Also, since the detection part can be accurately opposed and installed in the channel, the manufacture of the microchannel device becomes easy. Moreover, since the electrode pattern such as the detection part is formed on one electrode substrate, etching techniques, various printing techniques, etc. can be easily used for forming the electrode pattern, and from this point as well, the manufacture of the microchannel device becomes easy.

[0012] In addition to the above features, the present invention is characterized in that the electrode pattern formed on the electrode substrate includes a detection part formed on a surface facing the cover member side, a lead-out circuit pattern drawn from the detection part, and an input / output electrode part formed on the opposite surface via a through hole in the lead-out circuit pattern. According to the present invention, since the detection unit is formed on the surface facing the cover member side, the detection unit is located on the cover member close to the flow path, and an accurate detection output can be obtained. At the same time, since the electrode unit for input / output appears on the surface side of the micro flow path device, the detection output can be easily taken out to the outside.

[0013] In addition, the present invention is characterized in that, in addition to the above features, the electrode substrate is detachably attached to the cover member. According to the present invention, the electrode substrate can be repeatedly used (reused), and waste can be reduced.

[0014] In addition, the present invention provides a flow path body provided with a flow path formed of fine grooves in a substrate, a sample introduction unit for introducing a liquid sample for analysis into the flow path, and a sample recovery unit for recovering the liquid sample introduced into the flow path from the sample introduction unit and subjected to capillary electric induction. An electrode substrate is placed on a cover member covering the flow path of the flow path body as a separate body from the cover member. The electrode substrate is formed with an electrode pattern having a detection unit for analyzing a liquid sample by capillary electric induction at a position facing the flow path of the flow path body when placed on the cover member. According to the present invention, since the electrode pattern such as the detection unit is provided on an electrode substrate separate from the cover member and this electrode substrate is placed on the cover member, it is possible to accurately oppose the detection unit to the flow path of the micro flow path device configured by attaching this electrode substrate. Also, since the detection unit can be accurately opposed and installed to the flow path, a micro flow path device using this electrode substrate can be easily manufactured. In addition, since the electrode pattern such as the detection unit is formed on a single electrode substrate, etching techniques, various printing techniques, etc. can be easily used for forming the electrode pattern.

[0015] In addition to the above features, the present invention is characterized in that the electrode pattern includes a detection portion formed on a surface facing the cover member side, a lead-out circuit pattern drawn from the detection portion, and an input / output electrode portion formed on the opposite surface via a through hole in the lead-out circuit pattern. According to the present invention, since the detection portion is formed on the surface facing the cover member side, the detection portion can be positioned on the cover member close to the flow path, and an accurate detection output can be obtained from the micro flow path device to which this electrode substrate is attached. At the same time, since the input / output electrode portion can be positioned on the surface side of the micro flow path device, the detection output of the micro flow path device configured by attaching this electrode substrate can be easily taken out to the outside.

[0016] In addition, the present invention provides a micro flow path device main body in which a flow path formed of fine grooves in a substrate, a sample introduction portion for introducing a liquid sample for analysis into the flow path, and a sample recovery portion for recovering the liquid sample introduced into the flow path from the sample introduction portion and then subjected to capillary electrophoresis induction is covered with a cover member, and an electrode substrate on which an electrode pattern having a detection portion for liquid sample analysis by capillary electrophoresis induction is formed. The electrode substrate is detachably placed on the surface of the cover member. At this time, the micro flow path device is configured by positioning the detection portion of the electrode substrate at a position facing the flow path of the flow path substrate, and a method for using the micro flow path device characterized by performing analysis of a liquid sample by capillary electrophoresis induction. According to the present invention, since the micro flow path device is configured by detachably placing the electrode substrate on the surface of the cover member, the electrode substrate can be repeatedly used (reused).

Effects of the Invention

[0017] According to the present invention, the manufacture of the micro flow path device can be easily performed, an accurate output can be obtained, and some components can be repeatedly used, thereby reducing waste of the components.

Brief Description of the Drawings

[0018]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Embodiments for Carrying Out the Invention

[0019] Hereinafter, embodiments of the present invention will be described in detail with reference to the drawings. FIG. 1 is a perspective view of a microchannel device 1 according to an embodiment of the present invention, FIG. 2 is an enlarged plan view of the main part of the portion where the electrode substrate 51 of the microchannel device 1 is placed, FIG. 3 is an exploded perspective view of the microchannel device 1, and FIG. 4 is an exploded perspective view of the microchannel device 1 seen from below. As shown in these figures, the microchannel device 1 includes a channel body 11, a cover member 31 fixed on the channel body 11, an electrode substrate 51, and a fixing means 71. In the following description, "up" refers to the direction of viewing from the channel body 11 toward the cover member 31 side, and "down" refers to the opposite direction.

[0020] The flow path body 11 is configured to include a substrate 12 formed by molding a synthetic resin into a substantially flat plate-shaped substantially rectangular parallelepiped. In this embodiment, the substrate 12 uses an acrylic resin and has a thickness of about 1.5 mm. On the upper surface of the substrate 12, flow paths 13 (13a, 13b) formed of fine grooves are formed. The flow path 13 includes a flow path portion 13a that linearly extends in the longitudinal direction A of the substrate 12 from the center of the upper surface of the substrate 12, and a flow path portion 13b that linearly extends in the width direction B of the substrate 12 at one end side of the flow path portion 13a. The flow path portion 13a and the flow path portion 13b intersect (are orthogonal). The width and depth of the fine grooves constituting the flow path 13 are 10 μm to 250 μm, and in this example, they have a square cross-sectional shape of 100 μm × 100 μm. Further, at each end (four locations) of the flow path 13, liquid insertion portions 15a, 15b, 15c, 15d that penetrate vertically are formed. At positions surrounding the respective liquid insertion portions 15a, 15b, 15c, 15d on the lower surface side of the substrate 12, attachment portions 17a, 17b, 17c, 17d that protrude in a cylindrical shape are formed. Each of the attachment portions 17a, 17b, 17c, 17d is formed in a dimensional shape into which a sample injection (recovery) instrument (not shown) connected when introducing or recovering (discharging) liquid into the flow path 13 is inserted. In this example, hereinafter, the liquid insertion portion 15a is a buffer solution introduction portion, the liquid insertion portion 15b is a sample recovery portion, the liquid insertion portion 15c is a sample introduction portion, and the liquid insertion portion 15d is a liquid recovery portion, but there are also various other usage methods.

[0021] The cover member 31 is a synthetic resin film having the same outer shape dimensions as the flow path body 11, that is, a substantially rectangular parallelepiped shape and flexibility, so as to cover the upper surface of the flow path body 11, and the dimensions in both the longitudinal direction A and the width direction B are formed to be the same as those of the substrate 12. In this embodiment, the material of the cover member 31 uses the same acrylic resin as the flow path body 11.

[0022] The electrode substrate 51 is a flexible synthetic resin film having a substantially rectangular parallelepiped shape. The dimension in the width direction B thereof is the same as the dimension in the width direction B of the flow path main body 11 and the cover member 31, and the dimension in the longitudinal direction A thereof is formed to be shorter than the dimension in the longitudinal direction A of the flow path main body 11 and the cover member 31. In this embodiment, the material of the electrode substrate 51 is a polyimide resin. As shown in FIG. 2, the dimension in the longitudinal direction A of the electrode substrate 51 is formed to be the length dimension from one end side X1 on the liquid insertion portion 15b side, which is the sample collection portion of the flow path main body 11, to covering a part of the flow path portion 13a.

[0023] A pair of electrode patterns 53 and 55 are formed on the electrode substrate 51. Both electrode patterns 53 and 55 have the same shape, and include a pair of detection portions 53a and 55b formed on the surface of the electrode substrate 51 facing the cover member 31, lead-out circuit patterns 53b and 55b formed so as to be connected to the respective detection portions 53a and 55b, and electrode portions 53c and 55c for input / output formed on the opposite surface and connected to the respective lead-out circuit patterns 53b and 55b via through holes 53d and 55d.

[0024] FIG. 6 is an enlarged plan view of the main part of the detection portions 53a and 55a shown in FIG. 2. As shown in FIGS. 2 and 6, both of the pair of detection portions 53a and 55a are linear and have the same length, and are formed on the same straight line with a gap S1 therebetween. The width dimension L1 of both detection portions 53a and 55a is formed to be slightly larger than the width dimension L2 of the flow path portion 13a disposed therebelow (L1 > L2). The pair of lead-out circuit patterns 53b and 55b have the same length and are formed so as to bend substantially at a right angle from one end of the detection portions 53a and 55a and extend in a direction away from the flow path portion 13a. The electrode portions 53c and 55c are formed on the surface opposite to the surface on which the detection portions 53a and 55a and the like are formed, and are connected to the lead-out circuit patterns 53b and 55b by through holes 53d and 55d provided at the tips of the lead-out circuit patterns 53b and 55b. These electrode patterns 53 and 55 are arranged point-symmetrically about the center point of the gap S1.

[0025] The fixing means 71 is composed of a rectangular adhesive tape for fixing (temporarily fixing) the electrode substrate 51 on the cover member 31. As this adhesive tape, one that can be attached to and detached from the electrode substrate 51 and the cover member 31 is used.

[0026] Next, the assembling method of the microchannel device 1 will be described. First, as shown in FIG. 5, the cover member 31 is brought into close contact with the upper surface of the channel body 11 (the surface on which the channel 13 is formed). Next, the channel body 11 and the cover member 31 that are in close contact are thermocompression bonded by a heat roller (heat roller) or a hot press. Since the channel body 11 and the cover member 31 are made of synthetic resin of the same material, the two are firmly fixed. Note that no adhesive is used for fixing the channel body 11 and the cover member 31. The reason is that if an adhesive is used, there is a risk that the adhesive will enter the channel 13, and it is difficult to control the thickness of the layer due to the adhesive. In some cases, an adhesive may be used. The integrated unit of the channel body 11 and the cover member 31 is referred to as the microchannel device body 10.

[0027] Next, the electrode substrate 51 is placed on the upper surface of the cover member 31 integrated with the channel body 11 (the surface on the side not facing the channel 13). At this time, a pair of detection portions 53a, 55a are positioned so as to face the channel portion 13a on the channel portion 13a. That is, the detection portions 53a, 55a are directly above the linear channel portion 13a and are arranged along the channel portion 13a. The alignment of the channel portion 13a and the detection portions 53a, 55a is performed by, for example, aligning one end side X1 on the liquid insertion portion 15b side, which is the sample collection portion of the microchannel device body 10, with one end side Y1 of the electrode substrate 51 (alignment in the longitudinal direction A), and then moving the electrode substrate 51 in the width direction B using a microscope so that the detection portions 53a, 55a are positioned directly above the channel portion 13a (alignment in the width direction B). At this time, since the width dimension L1 of both detection portions 53a, 55a is formed to be slightly larger than the width dimension L2 of the channel portion 13a, the upper surface of the channel portion 13a can be easily and surely covered by both detection portions 53a, 55a.

[0028] When the alignment between the flow path portion 13a and the detection portions 53a and 55a is completed, the electrode substrate 51 is fixed onto the cover member 31 using the fixing means 71. This fixing is a temporary fixing.

[0029] Thereby, the microchannel device 1 shown in FIG. 1 is completed. It goes without saying that the above assembly procedure is an example, and it may be assembled using various other different assembly procedures.

[0030] Next, an example of the usage method of the microchannel device 1 will be described. First, in advance, measurement probes of a measuring device (not shown) are brought into contact with the terminal portions 53c and 55c of the electrode patterns 53 and 55 so that the capacitance (impedance) between the two electrode patterns 53 and 55 can be measured. Then, a buffer solution for electrophoresis is injected from the buffer solution introduction portion 15a on the side of the attachment portion 17a to fill the inside of the flow path 13, and a liquid sample (specimen) is injected from the sample introduction portion 15c on the side of the attachment portion 17c. Then, a high voltage is applied to the liquid sample and the buffer solution side using a high voltage system (not shown). Thereby, each component of the sample moves toward the sample recovery portion 15b side in the flow path portion 13a at a speed corresponding to its respective electrophoretic mobility. Then, the capacitance between a pair of electrode patterns 53 and 55 that changes according to the sample components that are separated and move according to the electrophoretic mobility is measured, and thereby each component in the liquid sample can be analyzed. Since the detection portions 53a and 55a for analyzing the liquid sample by electrophoresis can be arranged at a position in non-contact and close proximity to the liquid sample, highly accurate detection is possible also from this point. Further, since the detection portions 53a and 55a are provided closer to the sample recovery portion 15b than the midpoint of the region from the sample introduction portion 15c to the sample recovery portion 15b, the electrophoresis analysis can be performed with high accuracy at a position where the electrophoresis has progressed more.

[0031] The buffer solution and the liquid sample injected into the flow path 13 are recovered from the sample recovery portion 15b and the liquid recovery portion 15d. When the analysis of each component in the liquid sample is completed, the electrode substrate 51 is removed from the microchannel device main body 10 by peeling off the fixing means 71.

[0032] Since the microchannel device body 10 has been used, it is discarded. On the other hand, the electrode substrate 51 is attached to a new microchannel device body 10 using the same method as the assembly method of the microchannel device 1 to form a new microchannel device 1, and is used for analyzing another liquid sample or the like using the same usage method as above. Thus, the electrode substrate 51 can be repeatedly reused. Even when the electrode substrate 51 is repeatedly reused, since the width dimensions L1 of the detection portions 53a and 55a are formed to be slightly larger than the width dimension L2 of the channel portion 13a, the upper surface of the channel portion 13a can be easily and surely covered by both the detection portions 53a and 55a.

[0033] Also, since the width dimensions L1 of the detection portions 53a and 55a are formed to be slightly larger than the width dimension L2 of the channel portion 13a, this electrode substrate 51 can also be used for a channel body 11 having a channel 13a with a different channel width where the width of the channel 13a is slightly larger.

[0034] As described above, the microchannel device 1 includes a channel body 11 in which a channel 13 formed of a narrow groove, a sample introduction portion (liquid insertion portion) 15c for introducing a liquid sample for analysis into the channel 13, and a sample recovery portion (liquid insertion portion) 15b for recovering the liquid sample after being introduced into the channel 13 from the sample introduction portion 15c and being subjected to capillary electric induction; a cover member 31 laminated on the channel body 11 so as to cover the channel 13 of the channel body 11; and an electrode substrate 51 having electrode patterns 53 and 55 having detection portions 53a and 55a for liquid sample analysis by capillary electric induction, which are placed separately from the cover member 31 on the surface of the cover member 31 opposite to the channel body 11 and face the channel 13 of the channel body 11.

[0035] Thus, since the electrode patterns 53 and 55 such as the detection units 53a and 55a are provided on the electrode substrate 51 which is separate from the cover member 31 and this electrode substrate 51 is placed on the cover member 31, the detection units 53a and 55a can be accurately opposed to the flow path 13 and installed. Also, since the detection units 53a and 55a can be accurately opposed to the flow path 13 and installed, the manufacturing of the microchannel device 1 becomes easy.

[0036] Also, since the electrode patterns 53 and 55 such as the detection units 53a and 55a are formed on a single electrode substrate 51, etching techniques, various printing techniques, etc. can be easily used for forming the electrode patterns 53 and 55, and from this point as well, the manufacturing of the microchannel device 1 becomes easy.

[0037] Also, the microchannel device 1 is configured to include, on the electrode substrate 51, the detection units 53a and 55a formed on the surface facing the cover member 31 side, the lead-out circuit patterns 53b and 55b led out from the detection units 53a and 55a, and the input / output electrode portions 53c and 55c formed on the opposite surface via the through holes 53d and 55d in the lead-out circuit patterns 53b and 55b. Therefore, the detection units 53a and 55a are located on the cover member 31 close to the flow path 13, and an accurate detection output can be obtained. At the same time, since the input / output electrode portions 53c and 55c appear on the surface side of the microchannel device 1, the detection output can be easily taken out to the outside.

[0038] Also, since the electrode substrate 51 is detachably attached to the cover member 31, the electrode substrate 51 can be repeatedly used (reused), reducing waste.

[0039] Although the embodiments of the present invention have been described above, the present invention is not limited to the above embodiments, and various modifications are possible within the scope of the technical idea described in the claims, the specification, and the drawings. Note that any shape, structure, or material not directly described in the specification and the drawings is within the scope of the technical idea of the present invention as long as it exhibits the functions and effects of the present invention. For example, in the above embodiment, the flow path 13 is constituted by the flow path portions 13a and 13b that cross in a cross shape, but it goes without saying that various changes can be made to the configuration (number, shape, etc.) of the flow path.

[0040] Also, in the above embodiment, a synthetic resin film having flexibility is used as the cover member 31, but a rigid synthetic resin plate may also be used. Further, in the above embodiment, a synthetic resin film having flexibility is used as the electrode substrate 51, but a rigid synthetic resin plate may also be used. Various changes can also be made to the configuration (shape, number, etc.) of the electrode patterns 53 and 55 and the configuration (shape, structure, etc.) of the flow path body 11.

[0041] Also, in the above embodiment, an etching method is used to form the electrode patterns 53 and 55 on the electrode substrate 51, but they may be formed by other various methods such as screen printing of conductive ink, inkjet printing of conductive ink, vapor deposition, and sputtering methods.

[0042] Also, in the above embodiment, the materials of the flow path body 11 and the cover member 31 are made of the same acrylic resin, but various other homogeneous synthetic resins such as polycarbonate-based, polyolefin-based, polystyrene-based, and silicone resins such as PDMS may be used instead of acrylic. Similarly, various resins other than polyimide resin may be used for the electrode substrate 51.

[0043] In the above-described embodiment, an adhesive tape was used as the fixing means 71. However, any fixing means that detachably mounts the electrode substrate 51 on the cover member 31 may be used. For example, alignment holes may be provided in the microchannel device body 10 and the electrode substrate 51, and they may be aligned and mounted by passing pins through them, or they may be aligned and mounted using various other fixing means such as clips. In some cases, a fixing means that mounts and fixes the electrode substrate 51 on the cover member 31 so that it cannot be detached may also be used.

[0044] In addition, the embodiments described above and shown in the respective figures can be combined with each other's description content as long as there is no contradiction in their objectives and configurations, etc. Also, the description content of the above description and each figure can be independent embodiments even if it is a part of them, and the embodiments of the present invention are not limited to a single embodiment that combines the above description and each figure.

Description of Reference Numerals

[0045] 1 Microchannel device 11 Channel body 12 Substrate 13 Channel 15b Sample collection part (liquid insertion part) 15c Sample introduction part (liquid insertion part) 31 Cover member 51 Electrode substrate 53, 55 Electrode pattern 53a, 55a Detection part 53b, 55b Lead-out circuit pattern 53c, 55c Electrode part 53d, 55d Through hole 71 Fixing means

Claims

1. A flow channel body formed in a substrate, having a flow channel consisting of fine grooves, a sample introduction part for introducing a liquid sample for analysis into the flow channel, and a sample recovery part for recovering the liquid sample after being introduced into the flow channel from the sample introduction part and being subjected to capillary electrophoresis induction; A cover member laminated on the flow channel body so as to cover the flow channel of the flow channel body; An electrode substrate which is placed separately from the cover member on the surface of the cover member opposite to the flow channel body, and has an electrode pattern with a detection part for liquid sample analysis by capillary electrophoresis induction formed at a position facing the flow channel of the flow channel substrate; A micro flow channel device characterized by comprising the above.

2. The micro flow channel device according to Claim 1, wherein the electrode pattern formed on the electrode substrate comprises a detection part formed on the surface facing the cover member side, a lead-out circuit pattern drawn out from the detection part, and an electrode part for input / output formed on the opposite surface via a through hole in the lead-out circuit pattern. A micro flow channel device characterized by this.

3. The micro flow channel device according to Claim 1, wherein the electrode substrate is detachably attached to the cover member. A micro flow channel device characterized by this.

4. An electrode substrate placed separately from the cover member on a cover member covering the flow channel of a flow channel body provided with a flow channel consisting of fine grooves in a substrate, a sample introduction part for introducing a liquid sample for analysis into the flow channel, and a sample recovery part for recovering the liquid sample after being introduced into the flow channel from the sample introduction part and being subjected to capillary electrophoresis induction; The electrode substrate is characterized in that an electrode pattern having a detection part for liquid sample analysis by capillary electrophoresis induction is formed at a position facing the flow channel of the flow channel body when placed on the cover member so that the detection part is located there.

5. The electrode substrate according to Claim 4, wherein the electrode pattern is configured to include a detection part formed on the surface facing the cover member side, a lead-out circuit pattern drawn out from the detection part, and an electrode part for input / output formed on the opposite surface via a through hole in the lead-out circuit pattern. An electrode substrate characterized by this.

6. A microchannel device body is formed by covering a channel of a channel body, which is provided with a channel formed by fine grooves in a substrate, a sample introduction part for introducing a liquid sample for analysis into the channel, and a sample recovery part for recovering the liquid sample after being introduced into the channel from the sample introduction part and being capillary-electrically induced. An electrode substrate on which an electrode pattern having a detection part for analyzing a liquid sample by capillary electric induction is formed. Prepare A method of using a microchannel device, characterized in that the electrode substrate is detachably placed on the surface of the cover member, and at this time, the detection part of the electrode substrate is positioned at a position facing the channel of the channel substrate to constitute a microchannel device, and analysis of a liquid sample by capillary electric induction is performed.

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