Method for decomposing polycyclic aromatic hydrogen using decomposition bacterial flora complex system

Abstract

To provide a method for decomposing polycyclic aromatic hydrogen using a decomposition bacterial flora complex system.SOLUTION: A method for decomposing polycyclic aromatic hydrogen using a decomposition bacterial flora complex system is disclosed. The method includes a step of first, adding decomposition bacterial flora to organically contaminated water and reacting it for 1 to 48 hours, and subsequently adding a pre-complex and reacting it continuously until the total time reaches 72 hours, thereby forming a complex system of the decomposition bacterial flora and the pre-complex. This process can avoid the adverse effect of the pre-complex on the decomposition bacterial flora and successfully achieve decomposition. The present invention can effectively remove polycyclic aromatic hydrogen through the decomposition reaction by adding decomposition bacterial flora, a pre-complex of calcium peroxide and ferrous sulfate heptahydrate as a complex system to polycyclic aromatic hydrogen contaminated water. Compared to conventional microbial purification and oxidation purification, this method is efficient and environmentally friendly, and has high applicability.SELECTED DRAWING: Figure 1

Description

[Technical field]

[0001] The present invention relates to the technical field of purification of polluted water areas, specifically, to a method for the purification of polycyclic aromatic hydrocarbons using a complex of decomposing bacteria. The present invention relates to an improved method for the cracking of aromatic hydrogen. [Background technology]

[0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of toxic organic pollutants commonly found in aqueous phases. It is normally non-polar, hydrophobic, lipophilic, contains at least two benzene rings, and persists in the environment. These properties of polycyclic aromatic hydrocarbons can be used to purify polycyclic aromatic hydrocarbons in contaminated media. In most cases, biodegradation strategies are limited in their decomposition spectrum and are highly acidic. In addition, various environmental factors can reduce the efficiency of biological purification. Chemical purification techniques are expensive and prone to secondary pollution. In existing purification technologies for polycyclic aromatic hydrogen contaminated water areas, the decomposition efficiency of microorganisms is low and the decomposition spectrum is The torque is narrow, and the oxidation techniques are efficient and thorough, but expensive and highly toxic halogen-based. and nitro-based by-products. To improve the shortcomings of the technology, researchers have begun to focus on combining biological and oxidative purification. The researchers used a bacterial flora and calcium peroxide complex to measure the amount of polycyclic aromatic hydrocarbons (PAHs) in water. The technology to purify water uses free radicals generated in the decomposition of calcium peroxide and ferrous sulfate. However, there is a problem to be solved in that the growth of decomposition bacteria is inhibited by the fermentation process. Summary of the Invention

[0003] In order to solve the above problems, the present invention provides a method for the decomposition of polycyclic aromatic hydrocarbons using a decomposition bacterial consortium. A solution method is provided, which includes the following steps: S1. First, add a decomposition flora to the organic polluted water according to an addition amount of 1 to 11 wt%, and perform a decomposition reaction with a shaker at 150 rpm for 1 to 48 h. The organic polluted water is a water area polluted by polycyclic aromatic hydrocarbons. The decomposition flora is a mixture of three types of bacteria, namely Acinetobacter of Proteobacteria, Stenotrophomonas, and Comamonas, mixed at a weight ratio of 1:1:1. S2. Next, add the pre-complex. The addition amount of the pre-complex in 1 liter of the organic polluted water is 1.39 to 27.80 g. The decomposition flora and the pre-complex form a decomposition flora complex system, and continuously react with a shaker until the total time reaches 72 h to complete the decomposition. Here, the pre-complex is composed of calcium peroxide and ferrous sulfate heptahydrate, and the mass ratio of calcium peroxide to ferrous sulfate heptahydrate is 1 to 13.90:0.39 to 13.90. Note: The above method can solve the toxicity problem of the decomposition flora in the calcium peroxide + ferrous sulfate decomposition system, and has a good solution effect on solving the problems that the removal efficiency of organic pollutants in water, especially polycyclic aromatic hydrocarbons, is low and the decomposition spectrum is narrow by the conventional microbial decomposition technology. By utilizing the oxygen release property and oxidizing property of calcium peroxide, the decomposition ability of polycyclic aromatic hydrocarbons in water by the decomposition technology is significantly improved. The calcium peroxide + ferrous sulfate decomposition system has a good decomposition effect, but the free radicals generated in the calcium peroxide + ferrous sulfate decomposition system not only remove pollutants but also inhibit the growth of decomposition bacteria. By the above addition method and control of the reaction time, the above influence can be effectively reduced. Here, calcium peroxide The system is not only reinforcing but also complementary, and compared with conventional microbial purification or oxidation purification, this method is more efficient, environmentally friendly, and has high applicability. As one aspect of the present invention, in S1, a decomposition reaction is carried out with a shaker at 150 rpm for 24 h. Explanation: The above reaction time is the balance point for retaining decomposing bacteria while removing pollutants, and while removing pollutants, the survival rate of the decomposing bacteria increases. As one aspect of the present invention, in S1, the pH value of the organic polluted water is 5 - 7, and the multi- ring aromatic hydrocarbon is phenanthrene and pyrene. As one aspect of the present invention, in S2, for 1 liter of organic polluted water, the addition amount of calcium peroxide is 2.41 g, and the addition amount of ferrous sulfate heptahydrate is 7.51 g. As one aspect of the present invention, the preparation method of the pre-complex is as follows: S2-1. At 25°C, activated carbon and magnesium oxide with a particle size of 1 - 10 μm are mixed at a mass ratio of 3:0.5 to obtain a carrier. S2-2. At a ratio of 15 g:1 - 2 ml, hexadecylamine octanoate carbonate is sprayed on the surface of the carrier. S2-3. At a temperature of -15 to -10°C, the carrier and calcium peroxide are mixed at a weight ratio of 1 - 0.8:1 with the carrier sprayed with hexadecylamine octanoate carbonate and calcium peroxide, heated to 25°C, and dried at 25°C for 1 - 2 h to obtain a pre-complex. S2-4. Calcium peroxide and ferrous sulfate heptahydrate are mixed at a mass ratio of 1 - 13.90:0.39 - 13. 90 with the pre-complex and ferrous sulfate heptahydrate to form a pre-complex. Explanation: The pre-complex prepared by the above method provides a carrier for the reaction of organic pollutants with calcium peroxide and ferrous sulfate heptahydrate, thereby improving the decomposition effect of polluted water. Activated carbon and magnesium oxide are porous materials, and magnesium oxide has the effect of catalyzing the decomposition of compounds. Meanwhile, the use of the carrier can reduce the inhibitory effect of calcium peroxide and ferrous sulfate heptahydrate on the decomposing flora, and further improve the decomposition ability of the system. As one aspect of the present invention, in the above S2, the method for adding the pre-complex is as follows: Divide calcium peroxide and ferrous sulfate heptahydrate into three equal parts respectively. First, at 25°C, add one-third of calcium peroxide, and react with a shaker at 150 rpm for 5 - 15 min. Then add one-third of ferrous sulfate heptahydrate, and react with a shaker at 120 rpm for 3 - 4 h. Then lower the temperature from 25°C to 5 - 10°C, and at the same time add one-third of calcium peroxide and one-third of ferrous sulfate heptahydrate, and react with a shaker at 180 rpm for 2 - 3 h. Then add the remaining calcium peroxide, and react with a shaker at 150 rpm for 20 - 25 min. Add the remaining ferrous sulfate heptahydrate, and react with a shaker at 130 rpm until completion. Explanation: By the above addition method, the progress of the decomposition reaction can be promoted, the influence of the pre-complex on the decomposing bacteria can be further reduced, and the decomposition effect of the calcium peroxide + ferrous sulfate decomposition system can be better exerted. The above stepwise addition method can effectively slow down the time for forming the composite system of the two by adding ferrous sulfate heptahydrate and calcium peroxide and then reacting. At the same time, through the control of time and temperature, the promoting effect on the decomposition reaction of free radicals can be improved, and the killing effect on the decomposing flora can be reduced. As one aspect of the present invention, in the above decomposing flora, Acinetobacter of the genus Proteobacteria (A cinetobacter), Stenotrophomonas s), Comamonas are all domesticated bacteria, and the target pollutants to be decomposed are used as the only carbon source for domestication. As another preferred embodiment of the present invention, the degrading flora is obtained by screening and concentrating from long-term contaminated soil, and the long-term contaminated soil is polycyclic aromatic hydrocarbon contaminated soil with a contamination time of 2 years or more. Explanation: The degrading flora obtained by screening and concentrating the soil long-term contaminated by polycyclic aromatic hydrocarbons is more suitable for the degradation of related pollutants in water areas by the flora used. The pre-complex of the degrading flora and calcium peroxide (CP) is added to the polycyclic aromatic hydrocarbon contaminated water. After a certain reaction time, the polycyclic aromatic hydrocarbons can be removed with high efficiency. The screening and concentration method is as follows: S1-1, Preliminary domestication: Mix aromatic hydrocarbon contaminated soil, deionized water, and sodium sulfide at a mass ratio of 1:0.4:0.01 to 0.02 to obtain a mixture. Add the mixture to a mixed nutrient solution containing phenanthrene and pyrene at a mass ratio of 1:70 to 80, and perform preliminary domestication culture for 10 to 15 days. Then dry until the water content reaches 30 to 40% to obtain preliminary domesticated sludge. S1-2, Screening and concentration: Re-domesticate the preliminary domesticated sludge obtained in step S1-1. The re-domestication is as follows: Add the preliminary domesticated sludge to the mixed nutrient solution at a mass ratio of 1:60 to 65 to obtain a domesticated liquid. Culture with a shaker at 180 to 200 rpm, culture for 3 to 5 days per generation, and perform 5 to 6 subcultures. Culture until the content of phenanthrene in the domesticated liquid becomes 2.2 to 5 times the content of phenanthrene in the preliminary domesticated sludge. S1-3, Obtain the degrading flora: After the above steps, the degrading flora can be obtained. S1-1, Preliminary domestication: Mix aromatic hydrocarbon contaminated soil, deionized water, and sodium sulfide at a mass ratio of 1:0.4:0.01~ 0.02, obtain a mixture, and add the mixture to a mixed nutrient solution containing phenanthrene and pyrene at a mass ratio of 1:70~80, and perform preliminary domestication culture for 10~15d. Then dry until the water content reaches 30~40% to obtain preliminary domesticated sludge. S1-2, Screening and concentration: Re-domesticate the preliminary domesticated sludge obtained in step S1-1. The re-domestication is as follows: Add the preliminary domesticated sludge to the mixed nutrient solution at a mass ratio of 1:60~65 to obtain a domesticated liquid. Culture with a shaker at 180~200 rpm, culture for 3~5d per generation, and perform 5~6 subcultures. Culture until the content of phenanthrene in the domesticated liquid becomes 2.2~ 5 times the content of phenanthrene in the preliminary domesticated sludge. S1-3, Obtain the degrading flora: After the above steps, the degrading flora can be obtained. S1-1, Preliminary domestication: Mix aromatic hydrocarbon contaminated soil, deionized water, and sodium sulfide at a mass ratio of 1:0.4:0.01~ 0.02, obtain a mixture, and add the mixture to a mixed nutrient solution containing phenanthrene and pyrene at a mass ratio of 1:70~80, and perform preliminary domestication culture for 10~15d. Then dry until the water content reaches 30~40% to obtain preliminary domesticated sludge. S1-2, Screening and concentration: The domesticated liquid obtained in step S1-2 is inoculated into an inorganic salt medium containing 100-3000 mg / L phenanthrene / pyrene at an inoculation amount of 4-6% by mass fraction, and cultured with a shaker at 180-200 rpm for 24-64 h to obtain a decomposing bacterial flora. Explanation: The decomposing bacterial flora concentrated and domesticated by the above method has a good decomposing effect on polycyclic aromatic hydrocarbons. The growth of the bacterial flora is promoted by domesticating twice with a mixed nutrient solution, and a high efficiency biphenyl-decomposing bacterial flora can be concentrated. The amount of bacterial growth, bacterial flora diversity, and polycyclic aromatic hydrocarbon decomposition rate can be improved by using an inorganic salt medium. The composition of the mixed nutrient solution described in S1-1 and S1-2 is 1 g / L yeast extract, 5 g / L peptone, 1.6 g / L urea, 8 g / L K2HPO4, 1 L deionized water, 4 50 mg / L phenanthrene and 450 mg / L pyrene, with a pH of 7.0 ± 0.5. The composition of the inorganic salt medium is 1 g / L NH4Cl, 1 g / L K2HPO4, 0.2 g / L MgSO4 , 0.02 g / L FeSO4·7H2O, 1 g / L NaCl, 3 g / L (NH4)2SO4 , 0.01 g / L CaCl2, 4 mg / L MoO3, 28 mg / L ZnSO4·5H2O , 0.02 mg / L CuSO4·5H2O, 4 mg / L H3BO3, 4 mg / L MnSO4·5H2 O4, 4 mg / L CoCl2·6H2O, with a pH of 7.0 ± 0.5. The method of the present invention can solve the problem of toxicity to the decomposing bacterial flora in the decomposition system of calcium peroxide + ferrous sulfate, and has a good solution to the above problems that the removal efficiency of water organic pollutants, especially polycyclic aromatic hydrocarbons, is low and the decomposition spectrum is narrow in the conventional microbial decomposition technology. Through the oxygen release property and oxidizing property of calcium peroxide, water by the decomposition technology has a good solution effect, and through the oxygen release property and oxidizing property of calcium peroxide, water With the help of the decomposition ability of polycyclic aromatic hydrocarbons in it, it belongs to the field of contaminated water purification. Compared with the conventional microbial purification and oxidation purification, this method is more efficient, environmentally friendly, and has high applicability.

Brief Description of the Drawings

[0004]

Figure 1

Modes for Carrying Out the Invention

[0005] Hereinafter, in order to better reflect the advantages of the present invention, the present invention will be described in more detail in connection with specific embodiments. For more details. Example 1 The method for decomposing polycyclic aromatic hydrocarbons using a decomposed flora complex system includes the following steps: S1. First, add the decomposed flora to the organic contaminated water at an addition amount of 5 wt%, and use a shaker at 150 rpm for a 24-hour decomposition reaction. Here, the pH value of the organic contaminated water is 6, the organic contaminated water is a water area contaminated by polycyclic aromatic hydrocarbons, the polycyclic aromatic hydrocarbons are phenanthrene and pyrene, and the decomposed flora is a mixture of three types of bacteria, namely Acinetobacter, Stenotrophomonas, and Comamonas in the genus Proteobacteria, mixed at a weight ratio of 1:1:1. Acinetobacter in the genus Proteobacteria Stenotrophomonas, and Comamonas mixed at a weight ratio of 1:1:1. S2. Next, add the pre-complex. The addition amount of the pre-complex in 1 liter of organic contaminated water is 9.92 g. The decomposed flora and the pre-complex form a decomposed flora complex system, and continuously react with a shaker until the total time reaches 72 hours to complete the decomposition. Here, the pre-complex consists of calcium peroxide and ferrous sulfate heptahydrate. The mass ratio of lutetium to ferrous sulfate heptahydrate is 2.41:7.51.

[0006] Example 2 This example is different from Example 1 in the following aspects: In S1, the addition amount of the decomposing flora is 1 wt% of the organic polluted water. Example 3 This example is different from Example 1 in the following aspects: In S1, the addition amount of the decomposing flora is 11 wt% of the organic polluted water. Example 4 This example is different from Example 1 in the following aspects: In S1, a shaking machine at 150 rpm is used for a 1-hour decomposition reaction. Example 5 This example is different from Example 1 in the following aspects: In S1, a shaking machine at 150 rpm is used for a 48 h decomposition reaction. Example 6 This example is different from Example 1 in the following aspects: In S1, the pH value of the organic polluted water is 5. Example 7 This example is different from Example 1 in the following aspects: In S1, the pH value of the organic polluted water is 7. Example 8 This example is different from Example 1 in the following aspects: In S2, the addition amount of the pre-complex in 1 liter of organic polluted water is 1.39 g, where calcium peroxide is 1 g and ferrous sulfate heptahydrate is 0.39 g. Example 9 This example is different from Example 1 in the following aspects: In S2, the addition amount of the pre-complex in 1 liter of organic polluted water is 27.80 g, where calcium peroxide is 13.9 g and ferrous sulfate heptahydrate is 13.9 g. Example 10 This example is different from Example 1 in the following aspects: The components of the pre-complex are improved, and after improvement, the pre-complex ​​​​​​​​The combination contains two or more components of calcium peroxide and ferrous sulfate heptahydrate, and the modified pre-complex The preparation method is as follows: S2-1. At 25°C, activated carbon and magnesium oxide with a particle size of 1-10 μm are mixed at a mass ratio of 3:0.5 to obtain a carrier. S2-2. At a ratio of 15 g:1 ml, hexadecylamine octanoate is sprayed on the surface of the carrier. S2-3. At a temperature of -15°C, the carrier and calcium peroxide are mixed at a weight ratio of 1~:1 with the carrier sprayed with hexadecylamine octanoate carbonate, heated to 25°C, and dried at 25°C for 1 h to obtain a pre-complex. S2-4. Calcium peroxide and ferrous sulfate heptahydrate are mixed at a mass ratio of 1:0.39 with the above pre-complex and ferrous sulfate heptahydrate to form a pre-complex. Example 11 This example is different from Example 10 in that in S2-2, the ratio of the carrier to hexadecylamine octanoate carbonate is 15 g:2 ml. Example 12 This example is different from Example 10 in that in S2-2, the ratio of the carrier to hexadecylamine octanoate carbonate is 15 g:1.8 ml. Example 13 This example is different from Example 10 in that in S2-3, at a temperature of -12°C, the carrier and calcium peroxide are mixed at a weight ratio of 0.9:1 with the carrier sprayed with hexadecylamine octanoate carbonate, heated to 25°C, and dried at 25°C for 1.5 h to obtain a pre-complex. Example 14 This example is different from Example 10 in that in S2-3, at a temperature of -10°C, the carrier and calcium peroxide are mixed at a weight ratio of 0.8:1 with the carrier sprayed with hexadecylamine octanoate carbonate. ​​​​​​​​​​​ Mix the sprayed carrier with calcium peroxide, heat it to 25°C, and dry it at 25°C for 2 h to obtain the pre-complex. Example 15 This example is different from Example 10 in the following aspects. In S2-4, calcium peroxide and sulfur ferrous sulfate heptahydrate are mixed at a mass ratio of 3:1, and the pre-complex and ferrous sulfate heptahydrate are mixed to form a pre-complex. Example 16 This example is different from Example 10 in the following aspects. In S2-4, calcium peroxide and sulfur ferrous sulfate heptahydrate are mixed at a mass ratio of 1:1, and the pre-complex and ferrous sulfate heptahydrate are mixed to form a pre-complex. Example 17 This example is different from Example 1 in the following aspects. In S2, the addition method of the pre-complex is as follows: Divide calcium peroxide and ferrous sulfate heptahydrate into three equal parts each. First, at 25°C, add one-third of calcium peroxide, react for 10 min with a shaker at 150 rpm, then add one-third of ferrous sulfate heptahydrate, react for 3.5 h with a shaker at 120 rpm, then lower the temperature from 25°C to 8°C, and at the same time add one-third of calcium peroxide and one-third of ferrous sulfate heptahydrate, react for 2.5 h with a shaker at 180 rpm, then add the remaining calcium peroxide, react for 23 min with a shaker at 150 rpm, and then add the remaining ferrous sulfate heptahydrate, and react with a shaker at 130 rpm until completion. Example 18 This example is different from Example 17 in the following aspects. In S2, the addition method of the pre-complex is as follows: Divide calcium peroxide and ferrous sulfate heptahydrate into three equal parts each. First, at 25°C, add one-third of calcium peroxide, react for 5 min with a shaker at 150 rpm, Add, and then add one-third of ferrous sulfate heptahydrate, and react for 3 h with a shaker at 120 rpm. Then lower the temperature from 25 °C to 5 °C, and at the same time add one-third of calcium peroxide and one-third of ferrous sulfate heptahydrate, react for 2 h with a shaker at 180 rpm, and then add the remaining calcium peroxide, react for 20 min with a shaker at 150 rpm, add the remaining ferrous sulfate heptahydrate, and react until completion with a shaker at 130 rpm. Example 19 This example is different from Example 17 in the following aspects. In S2, the addition method of the pre-complex is as follows: Divide calcium peroxide and ferrous sulfate heptahydrate into three equal parts respectively. First, at 25 °C, add one-third of calcium peroxide, react for 15 min with a shaker at 150 rpm, then add one-third of ferrous sulfate heptahydrate, react for 4 h with a shaker at 120 rpm, then lower the temperature from 25 °C to 10 °C, and at the same time add one-third of calcium peroxide and one-third of ferrous sulfate heptahydrate, react for 3 h with a shaker at 180 rpm, and then add the remaining calcium peroxide, react for 25 min with a shaker at 150 rpm, add the remaining ferrous sulfate heptahydrate, and react until completion with a shaker at 130 rpm. Example 20 This example is different from Example 1 in the following aspects. The screening and enrichment of the degrading flora are as follows : S1-1, Preliminary acclimation: Mix polycyclic aromatic hydrocarbon-contaminated soil with a pollution time of 2 years, deionized water, and sodium sulfide at a mass ratio of 1:0 .4:0.015 to obtain a mixture. Add the mixture to a mixed nutrient solution containing phenanthrene and pyrene at a mass ratio of 1:75, and perform preliminary acclimation culture for 12 d. Then dry until the water content reaches 35% to obtain preliminarily acclimated sludge. S1-2, Screening and Concentration: The preliminary acclimated sludge obtained in step S1-1 is acclimated again. The re-acclimation is as follows : Add the preliminary acclimated sludge to the mixed nutrient solution at a mass ratio of 1:62 to obtain an acclimated liquid, and culture it with a shaker at 190 rpm , culture for 4 days for each generation, perform 5 subcultures, and culture until the phenanthrene content in the acclimated liquid reaches 3 times the phenanthrene content in the preliminary acclimated sludge . The composition of the mixed nutrient solution described in step S1-1 and step S1-2 is 1 g / L of yeast extract, 5 g / L of peptone , 1.6 g / L of urea, 8 g / L of K2HPO4, 1 L of deionized water, 450 mg / L of phenanthrene and 450 mg / L of pyrene, and the pH is 7.0 ± 0.5 . S1-3, Obtaining a Degrading Bacterial Community: Inoculate the acclimated liquid obtained in step S1-2 into an inorganic salt medium containing 2000 mg / L of phenanthrene / pyrene at an inoculation amount of 5% by mass fraction, and culture with a shaker at 190 rpm for 50 h to obtain a degrading bacterial community. As a result of measurement, the obtained degrading bacterial community contains three main bacteria, Acinetobacter, Stenotrophomonas, and Comamonas . The composition of the degrading bacterial community is shown in Figure 1 . The composition of the inorganic salt medium is 1 g / L of NH4Cl, 1 g / L of K2HPO4, 0.2 g / L of MgSO4 , 0.02 g / L of FeSO4·7H2O, 1 g / L of NaCl, 3 g / L of (NH4)2SO4 , 0.01 g / L of CaCl2, 4 mg / L of MoO3, 28 mg / L of ZnSO4·5H2O , 0.02 mg / L of CuSO4·5H2O, 4 mg / L of H3BO3, 4 mg / L of MnSO4·5H2O , and 4 mg / L of CoCl2·6H2O, and the pH is 7.0 . Example 21 This example is different from Example 20 in the following points. In step S1-1, aromatic hydrocarbons . . . . ​​​​Mix pristine contaminated soil, deionized water, and sodium sulfide at a mass ratio of 1:0.4:0.01 , to obtain a mixture, and add the mixture to a mixed nutrient solution containing phenanthrene and pyrene at a mass ratio of 1:70, and perform preliminary acclimation culture for 10 d. Then dry it until the water content reaches 30% to obtain preliminarily acclimated sludge. Example 22 This example is different from Example 20 in the following points. In step S1-1, mix pristine contaminated soil, deionized water, and sodium sulfide at a mass ratio of 1:0.4:0.02 , to obtain a mixture, and add the mixture to a mixed nutrient solution containing phenanthrene and pyrene at a mass ratio of 1:80, and perform preliminary acclimation culture for 15 d. Then dry it until the water content reaches 40% to obtain preliminarily acclimated sludge. Example 23 This example is different from Example 20 in the following points. The culture parameters in step S1-2 are different. Add the preliminarily acclimated sludge to the mixed nutrient solution at a mass ratio of 1:65 to obtain an acclimated liquid. Culture it with a shaker at 200 rpm, culture for 3 d per generation, and perform 5 subcultures until the content of phenanthrene in the acclimated liquid becomes 4.5 times that of the phenanthrene content in the preliminarily acclimated sludge . Example 24 This example is different from Example 20 in the following points. The culture parameters in step S1-2 are different. Add the preliminarily acclimated sludge to the mixed nutrient solution at a mass ratio of 1:60 to obtain an acclimated liquid. Culture it with a shaker at 180 rpm, culture for 5 d per generation, and perform 6 subcultures until the content of phenanthrene in the acclimated liquid becomes 2.0 times that of the phenanthrene content in the preliminarily acclimated sludge . Example 25 This example is different from Example 20 in the following points. The culture parameters in step S1-3 are different . Example 24 This example is different from Example 20 in the following points. The culture parameters in step S1-2 are different . Add the preliminarily acclimated sludge to the mixed nutrient solution at a mass ratio of 1:60 to obtain an acclimated liquid. Culture it with a shaker at 180 rpm, culture for 5 d per generation, and perform 6 subcultures until the content of phenanthrene in the acclimated liquid becomes 2.0 times that of the phenanthrene content in the preliminarily acclimated sludge . Example 25 This example is different from Example 20 in the following points. The culture parameters in step S1-3 are different Example 25 This example is different from Example 20 in the following points. The culture parameters in step S1-3 are different The conditioned liquid obtained in step S1-2 was inoculated at a mass fraction of 4% to 3000 mg / Inoculate the inorganic salt medium containing L-phenanthrene / pyrene and incubate for 24 h on a shaker at 200 rpm. h Cultivate to obtain a decomposition flora. Example 26 This embodiment differs from Example 20 in the following respects: the culture parameters in step S1-3 are different. The conditioned liquid obtained in step S1-2 was inoculated at a mass fraction of 6% at 100 mg / L. Inoculate the medium containing phenanthrene / pyrene and incubate for 64 h on a shaker at 180 rpm. The decomposition bacteria are obtained by culturing. Experimental Example 1. Examination of the decomposition efficiency of the present invention against polycyclic aromatic hydrogen in organically contaminated water; Here, the decomposition rate of polycyclic aromatic hydrogen in water is calculated by the following formula: JPEG2025106785000002.jpg151471, Effects of the amount of decomposition bacteria and precomplex added on the decomposition efficiency of polycyclic aromatic hydrocarbons in organic polluted water Research, Comparative Example 1: Comparative Example 1 differs from Example 1 in the following respects: , without adding ferrous sulfate heptahydrate, only 5 wt% of the decomposition bacteria flora was added to decompose the material. Comparative Example 2: Comparative Example 2 differs from Example 1 in the following respects: decomposition bacteria were added to the organic polluted water. First, 2 g of calcium peroxide and 8.34 g of ferrous sulfate heptahydrate were added to decompose the solution. Table 1. Effect of the amount of decomposition bacteria added on the decomposition efficiency of polycyclic aromatic hydrogens TIFF2025106785000003.tif85159 As can be seen from Table 1, the results of comparing Example 1, Comparative Example 1, and Comparative Example 2 are as follows: Compared with the single purification technology of the microbial decomposition and CP system used alone in Example 2, In the composite technology of Example 1, the decomposition efficiency of polycyclic aromatic hydrogen in contaminated water is 29.97 to 59.5 It increases by 1%, indicating that the method of Example 1 has the advantage of conforming to the system. Therefore, the method of Example 1 is more preferable. As a result of comparing Example 1, Example 2, and Example 3, when the addition amount of the decomposing flora is 5 wt%, the decomposition effect of polycyclic aromatic hydrocarbons is the best. This is because when the addition amount of the decomposing flora is low, the amount of the decomposing bacteria is insufficient and the decomposition effect is low. When the addition amount of the decomposing flora is further increased, the decomposition effect does not improve accordingly. Therefore, from an economic perspective, the parameters of Example 1 are more preferable. 2. During the treatment, investigate the influence of the addition method of the pre-complex and the decomposing flora on the decomposition efficiency of polycyclic aromatic hydrocarbons in organic polluted water. Comparative Example 3: It is different from Example 1 in the following points. First, the pre-complex is added and reacted for 24 h, then the decomposing flora is added and the reaction is continued until the total time reaches 72 h to complete the decomposition. Table 2 Influence of the method of the decomposing flora and the pre-complex on the decomposition efficiency of polycyclic aromatic hydrocarbons TIFF2025106785000004.tif85160 As can be seen from Table 2, as a result of comparing Example 1 and Comparative Example 3, the addition order of Example 1 is more preferable. The decomposition order has a great influence on the decomposition of aromatic hydrocarbons. Example 1 is more preferable. As a result of comparing Example 1, 17, 18, and 19, the composite systems prepared by the methods of Example 17 to 19 have good decomposition effects on pyrene and phenanthrene. This is because by using the carriers of Example 17 to 19, the decomposition effect of polluted water is improved. The porous materials of activated carbon and magnesium oxide have the effect of catalyzing and promoting the decomposition. Therefore, the decomposition ability of the composite system is strong. Here, Example 19 is more preferable. 3. Investigate the influence of the pH value of organic polluted water on the decomposition efficiency of polycyclic aromatic hydrocarbons. Comparative Example 4: It is different from Example 1 in the following points. The pH value of the organic polluted water is 3, Comparative Example 5: It is different from Example 1 in the following points. The pH value of the organic polluted water is 11, Table 3 Influence of pH Value of Organic Polluted Water on the Degradation Efficiency of Polycyclic Aromatic Hydrocarbons TIFF2025106785000005.tif83158 As can be seen from Table 3, Example 1, Example 6 and Example 7 were compared with Comparative Example 4 and Comparative Example 5 The results show that when the pH in Examples 1, 6 and 7, that is, the pH value is 5 - 7 , the degradation efficiency of the pre - complex is the best. When Example 4 was compared with Comparative Example 4 and Comparative Example 5, the results show that whether the pH value is too low or too high, the degradation efficiency of the system is inferior, but still maintains a certain ability to degrade polycyclic aromatic hydrocarbons. 4. Investigate the influence of the components of the decomposing bacterial flora on the degradation efficiency of polycyclic aromatic hydrocarbons in organic polluted water, Comparative Example 6: The decomposing bacterial flora adopted existing PAHs - degrading strains, cited from "Research Progress on Polycyclic Aromatic Hydrocarbon - Degrading Bacteria and Their Applications" Table 4 Influence of the Components of the Decomposing Bacterial Flora on the Degradation Efficiency of Polycyclic Aromatic Hydrocarbons TIFF2025106785000006.tif60158 As can be seen from Table 4, when Example 1 was compared with Comparative Example 6, the degradation effect of the decomposing bacterial flora adopted in Example 1 was more favorable. When Example 1 was compared with Example 20, the results show that in Example 20 , the degradation effect of polycyclic aromatic hydrocarbons in the water area using the domesticated and concentrated decomposing bacterial flora was also good. II. Investigate the anti - interference performance of the degradation treatment in Example 1, Using two kinds of dissolved organic matters, fulvic acid and humic acid respectively, investigate the degradation ability of the pre - complex , Comparative Example 7: It is different from Example 1 in the following points. 100 mg / L of fulvic acid was added to the organic polluted water for decomposition Comparative Example 8: Different from Example 1 in the following points, 250 mg / L of fulvic acid was added to the organic polluted water and decomposed. Comparative Example 9: Different from Example 1 in the following points, 100 mg / L of humic acid was added to the organic polluted water and decomposed. Comparative Example 10: Different from Example 1 in the following points, 250 mg / L of humic acid was added to the organic polluted water and decomposed. Table 5 Anti-interference performance of decomposition treatment TIFF2025106785000007.tif79160 As can be seen from Table 5, as a result of comparing Example 1, Comparative Example 7 and Comparative Example 8, fulvic acid reduces the decomposition efficiency of polycyclic aromatic hydrocarbons by the system and as a result of comparing Example 1, Comparative Example 9 and Comparative Example 10, humic acid also reduces the decomposition efficiency of polycyclic aromatic hydrocarbons by the system, but the degree of reduction of both is limited, and the pre-complex still maintains a certain decomposition ability. However, the degree of their reduction is limited, and the pre-complex still maintains a certain decomposition ability. ​

Claims

1. S1. First, add a decomposition bacterial flora to the organic polluted water according to an addition amount of 1 to 11 wt%, and perform a decomposition reaction for 1 to 48 h with a shaker at 150 rpm. The organic polluted water is a water area polluted by polycyclic aromatic hydrocarbons. The decomposition bacterial flora is a step of mixing three types of bacteria, namely, Acinetobacter of Proteobacteria, Stenotrophomonas, and Comamonas, in a weight ratio of 1:1:1, and S2. Next, add the pre-complex. The addition amount of the pre-complex in 1 liter of the organic polluted water is 1.39 to 27.80 g. The decomposition bacterial flora and the pre-complex form a decomposition bacterial flora complex system, and continuously react with a shaker until the total time reaches 72 h to complete the decomposition. The method includes the steps of, where the pre-complex consists of calcium peroxide and ferrous sulfate heptahydrate, and the mass ratio of calcium peroxide to ferrous sulfate heptahydrate is 1 to 13.90:0.39 to 13.

90. A method for decomposing polycyclic aromatic hydrocarbons using a decomposition bacterial flora complex system, characterized in that it is as described above.

2. In S1, the decomposition reaction is performed for 24 h with a shaker at 150 rpm, which is characterized in that the method according to claim 1 is as described above.

3. In S1, the pH value of the organic polluted water is 5 to 7, and the polycyclic aromatic hydrocarbons are phenanthrene and pyrene, which is characterized in that the method according to claim 1 is as described above.

4. In S2, in 1 liter of the organic polluted water, the addition amount of calcium peroxide is 2.41 g, and the addition amount of ferrous sulfate heptahydrate is 7.51 g, which is characterized in that the method according to claim 1 is as described above.

5. 、 The preparation method of the pre-complex is as follows: S2-1. At 25°C, mix activated carbon and magnesium oxide with a particle size of 1 to 10 μm in a mass ratio of 3:0.5 to obtain a carrier, and S2-2. Spray hexadecylamine octanoate on the surface of the carrier at a ratio of 15 g:1 to 2 ml, and S2-3. Under a temperature of -15 to -10°C, mix the carrier and calcium peroxide with a weight ratio of 1 to 0.8:1 with the carrier sprayed with hexadecylamine octanoate and calcium peroxide, heat to 25°C, and dry at 25°C for 1 to 2 h to obtain a pre-complex, and S2-4. Mix calcium peroxide and ferrous sulfate heptahydrate in a mass ratio of 1 to 13.90:0.39 to 13.90 to mix the pre-complex and ferrous sulfate heptahydrate to form a pre-complex. ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ ​ The method according to claim 1, characterized in that...

6. In S2, the method of adding the pre-complex is as follows: calcium peroxide, sulfuric acid Divide ferrous sulfate heptahydrate into three equal parts. First, add one-third of calcium peroxide at 25°C and react for 5 - 15 min with a shaker at 150 rpm. Next, add one-third of ferrous sulfate heptahydrate and react for 3 - 4 h with a shaker at 120 rpm. Then lower the temperature from 25°C to 5 - 10°C and simultaneously add one-third of calcium peroxide and one-third of ferrous sulfate heptahydrate and react for 2 - 3 h with a shaker at 180 rpm. Then add the remaining calcium peroxide and react for 20 - 25 min with a shaker at 150 rpm. Add the remaining ferrous sulfate heptahydrate and react with a shaker at 130 rpm until completion. The method according to claim 1, characterized in that... method.

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