Complex for preparation of liposomes

Abstract

To provide a complex which enables simple preparation of liposomes whose surfaces are positively charged and which pose no safety concern when used in cosmetics or skin external agents.SOLUTION: A complex for preparing liposomes is used that has positively charged surfaces, the complex being obtained by removing an organic solvent from a solution in which the following components (A) and (B) are uniformly dissolved in the organic solvent, and causing the components (A) and (B) to be simultaneously precipitated. The complex for preparing liposomes of the present invention enables simple preparation of liposomes having positively charged surfaces by dispersing the complex in water. (A) Phospholipids. (B) Sphingosines.SELECTED DRAWING: None

Description

Technical Field

[0001] The present invention relates to a complex for preparing positively charged liposomes containing phospholipids and sphingosines.

Background Art

[0002] Liposomes are spherical closed vesicles (vesicles) composed of a lipid bilayer membrane formed mainly of phospholipids, and can encapsulate lipophilic active ingredients inside the membrane and hydrophilic active ingredients in the inner aqueous phase. Liposomes are excellent in terms of usability and moisturizing effect, and also enhance the permeability of the encapsulated active ingredients into the skin, and have thus been used in cosmetics and topical skin preparations for a long time. Generally, liposomes formed from phospholipids such as lecithin have a negatively charged surface, but in recent years, attention has been paid to the fact that the skin surface is negatively charged, and attempts have been made to positively charge the liposome surface to improve the penetration of active ingredients into the skin by electrostatic interaction. For example, Patent Document 1 describes that liposomes with a positive zeta potential containing a cationic surfactant such as N-cocoyl-L-arginine ethyl ester pyrrolidone carboxylate are excellent in the skin permeability of active ingredients. Patent Document 2 describes that cationic liposomes containing a cationic lipid such as 1,2-dioleoyl-3-trimethylammonium propane (DOTAP) increase the skin absorption ability of active ingredients. However, the compounds used to impart a positive charge to the liposome surface in these documents are components corresponding to surfactants, and there are concerns about safety to the skin such as irritation, so there is a desire for positively charged liposomes without such concerns.

[0003] Sphingosines have various effects and efficacies, such as moisturizing, anti-acne, anti-inflammatory, and normalizing epidermal keratinization, and are a type of active ingredient that has long been used in cosmetics and topical skin preparations, with no safety concerns (e.g., Patent Document 3). However, no known attempts have been made in the field of cosmetics or topical skin preparations to encapsulate sphingosines in liposomes for use. Furthermore, because liposome preparation generally requires complicated procedures, there is a need for a simple method for preparing liposomes. Furthermore, there is a need for commercially available raw materials that enable the simple preparation of liposomes.

[0004] Patent Document 4 discloses a lecithin-sterol complex as a method for dispersing poorly hydratable lecithin in water, which is obtained by removing the organic solvent from a solution in which lecithin and sterol are uniformly dissolved in an organic solvent, thereby simultaneously precipitating the lecithin and sterol. This complex allows the preparation of a solubilized composition using lecithin without the need for a special preparation method, and the resulting composition has good dispersion stability. Patent Document 5 also discloses a poorly water-soluble physiologically active substance-phospholipid complex obtained by removing the organic solvent from a solution in which a poorly water-soluble physiologically active substance and phospholipid are uniformly dissolved in an organic solvent, thereby simultaneously precipitating the poorly water-soluble physiologically active substance and phospholipid. However, a complex containing a phospholipid and a sphingosine, and the use of this complex for preparing liposomes with a positively charged surface, are not known. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] International Publication No. 2014 / 069631 [Patent Document 2] Special Publication No. 2022-523422 [Patent Document 3] Japanese Patent Application Publication No. 05-085924 [Patent Document 4] Japanese Patent Application Publication No. 4-149194 [Patent Document 5] Patent Publication No. 2007-197328 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of the present invention is to provide a complex that can be easily prepared to produce liposomes with a positively charged surface that are free from safety concerns when used in cosmetics or topical skin preparations. [Means for solving the problem]

[0007] As a result of extensive research into solving the above-mentioned problems, the present inventors have found that the above-mentioned problems can be solved by a complex for preparing liposomes having a positively charged surface, which is obtained by removing the organic solvent from a solution in which component (A) a phospholipid and component (B) a sphingosine are uniformly dissolved in the organic solvent, thereby simultaneously precipitating components (A) and (B), and have thus completed the present invention. [Effects of the Invention]

[0008] The complex of the present invention can be dispersed in water to easily prepare liposomes with a positively charged surface. Furthermore, liposomes prepared by the complex of the present invention are safe for use in cosmetics or topical skin preparations, and because of their positively charged surfaces, they have excellent skin permeability for active ingredients. They also have an excellent feel when used, such as easy application, no stickiness when drying, and a moist feeling after drying. DETAILED DESCRIPTION OF THE INVENTION

[0009] The present invention relates to a composite obtained by simultaneously precipitating components (A) and (B) by removing the organic solvent from a solution in which the following components (A) and (B) are uniformly dissolved in the organic solvent. (A) Phospholipids (B) Sphingosines

[0010] Examples of phospholipids used as component (A) in the complex of the present invention include diacylglycerophospholipids having a polar group, such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, and phosphatidic acid; lecithin obtained from plants such as soybean, rapeseed, sunflower, safflower, peanut, cottonseed, corn, rice, and barley, and egg yolk, and hydrogenated versions thereof. These may also contain monoacylglycerophospholipids or lysolecithin. Furthermore, these may be derivatives modified with a polyoxyalkylene group such as polyethylene glycol. These phospholipids may be used alone or in combination of two or more. Of these, lecithin and hydrogenated versions thereof are preferred from the viewpoints of availability and enhancing the effects of the present invention. Lecithins with different phosphatidylcholine (hereinafter referred to as PC) contents depending on the degree of purification are commercially available, but from the viewpoint of providing the dispersion stability of liposomes described below and better exerting the effects of the present invention, it is recommended to use lecithins with a PC content of 60% by mass or more, preferably 70% by mass or more, and more preferably 90% by mass or more.

[0011] In the present invention, component (A) is used as the main component that forms the liposome membrane. Component (A) is contained in the complex in an amount of 40% by mass or more, preferably 50% by mass or more, and more preferably 60% by mass or more. If the content of component (A) is less than this range, liposomes may not be formed sufficiently.

[0012] The sphingosines of component (B) used in the complex of the present invention include sphingosine, dihydrosphingosine, phytosphingosine, sphingadienine, and their N-methyl or N,N-dimethyl derivatives. These may be used alone or in combination of two or more. Of these, from the viewpoint of further exerting the effects of the present invention, sphingosine, dihydrosphingosine, and phytosphingosine are preferred, and phytosphingosine is most preferred.

[0013] In the present invention, component (B) is used as a liposome membrane component for the purpose of positively charging the liposome surface. Further, component (B) also acts as an active ingredient for the skin. In the present invention, component (B) may be blended within the range in which liposomes with a positively charged surface are formed, and there is no particular limitation on the blending amount thereof, but the specific blending amount will be described below. Here, the surface of liposomes formed by lecithin is generally negatively charged, and since PC is a neutral phospholipid, the negative charge of liposomes formed by lecithin is due to acidic phospholipids other than PC contained in lecithin. Therefore, when lecithin is used as component (A) of the present invention, the blending amount of component (B) required to positively charge the liposome surface varies depending on the PC content of the lecithin used.

[0014] When lecithin with a PC content of 90% by mass or more is used as component (A), component (B) may be blended in an amount of 0.2 parts by mass or more, preferably 0.5 parts by mass or more, more preferably 1 part by mass or more, per 100 parts by mass of component (A). When lecithin with a PC content of 80 to 90% by mass is used as component (A), component (B) may be blended in an amount of 1 part by mass or more, preferably 2 parts by mass or more, more preferably 3 parts by mass or more, per 100 parts by mass of component (A). When lecithin with a PC content of 70 to 80% by mass is used as component (A), component (B) may be blended in an amount of 3 parts by mass or more, preferably 5 parts by mass or more, more preferably 7 parts by mass or more, per 100 parts by mass of component (A). When lecithin with a PC content of 60 to 70% by mass is used as component (A), component (B) may be blended in an amount of 7 parts by mass or more, preferably 10 parts by mass or more, more preferably 15 parts by mass or more, per 100 parts by mass of component (A). By setting the blending ratio of component (B) to component (A) as described above, liposomes with a positively charged surface can be obtained.

[0015] On the other hand, regarding the upper limit of the blending amount of component (B) in the present invention, there is no particular limitation. However, regardless of the PC content of the lecithin used, component (B) is preferably 40 parts by mass or less, more preferably 35 parts by mass or less, and most preferably 30 parts by mass or less with respect to 100 parts by mass of component (A). When the blending amount of component (B) is more than this, component (B) may not be retained within the liposome membrane and may precipitate over time.

[0016] As described above, in the present invention, in order to obtain positively charged liposomes, the lower the PC content of the lecithin, the more necessary it is to increase the blending amount of component (B). Even when lecithin with a PC content of less than 60% by mass is used as component (A), positively charged liposomes can be obtained by further increasing the blending amount of component (B). However, as the PC content of the lecithin used as component (A) decreases, the dispersion stability of the liposomes tends to decrease. Therefore, as the lecithin, it is preferable to use those with a PC content of 60% by mass or more, more preferably 70% by mass or more, and most preferably 90% by mass or more.

[0017] The complex of the present invention may further contain component (C) sterols for the purpose of improving the dispersion stability of the liposomes. Specifically, as the sterols, animal-derived sterols such as cholesterol, dihydrocholesterol, lanosterol, dihydrolanosterol, desmosterol; plant-derived sterols such as stigmasterol, sitosterol, campesterol, brassicasterol, and phytosterol which is a mixture of these; microorganism-derived sterols such as ergosterol; γ-oryzanol; and esterified products thereof can be mentioned. These sterols may be used alone or in combination of two or more. Among these, from the viewpoint of fully exerting the effects of the present invention, cholesterol, phytosterol, and γ-oryzanol are preferably mentioned. Component (C) is preferably contained in the complex in an amount of 1 to 39% by mass, more preferably 2 to 35% by mass, and still more preferably 3 to 30% by mass.

[0018] When the complex of the present invention is commercially available as a raw material for liposome preparation, it will be stored for a long period of time during the distribution process. Therefore, it is important to have excellent long-term storage stability. From such a viewpoint, it is preferable that the complex of the present invention further contains an organic acid as component (D). By containing component (D), precipitation of highly crystalline component (B), sphingosines, over time can be suppressed, and the storage stability of the complex of the present invention can be improved. It is presumed that component (D) suppresses the precipitation of component (B) over time because component (D) protects the amino group of component (B), thereby reducing its crystallinity.

[0019] Examples of the organic acid of component (D) used in the complex of the present invention include acetic acid, propionic acid, butyric acid, valeric acid, caproic acid, benzoic acid, salicylic acid, gallic acid, oxalic acid, malonic acid, succinic acid, glutaric acid, adipic acid, maleic acid, fumaric acid, phthalic acid, glycolic acid, lactic acid, malic acid, tartaric acid, citric acid, gluconic acid, levulinic acid, ketoglutaric acid, glutamic acid, aspartic acid, phytic acid, etc. These may be used alone or in combination of two or more. Among these, benzoic acid, succinic acid, lactic acid, malic acid, citric acid, glutamic acid, aspartic acid, phytic acid are preferable, and lactic acid, malic acid, citric acid are more preferable.

[0020] Regarding the blending amount of component (D) in the complex of the present invention, from the viewpoint of further improving the storage stability, based on 1 mol of component (B), component (D) is 0.5 mol or more, preferably 0.6 mol or more, more preferably 0.7 mol or more. On the other hand, although there is no particular limitation on the upper limit of the blending amount of component (D), based on 1 mol of component (B), component (D) is 10 mol or less, preferably 5 mol or less, more preferably 3 mol or less.

[0021] The complex of the present invention can further contain an oil-soluble active ingredient generally encapsulated within the liposome membrane, as long as the effects of the present invention are not impaired. Examples of the oil-soluble active ingredient include ceramides such as ceramide 1, ceramide 2, ceramide 3, ceramide 4, ceramide 5, ceramide 6, ceramide 7; carotenoids such as carotene, lycopene, cryptoxanthin, lutein, zeaxanthin, astaxanthin, crocetin, fucoxanthin; tocopherols such as α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, γ-tocotrienol, δ-tocotrienol, tocopherol acetate, tocopherol nicotinate; retinols such as retinol, retinol acetate, retinol palmitate, retinoic acid, retinoic acid tocopheryl, retinal; lipoic acid, coenzyme Q10, ferulic acid, ursolic acid, glycyrrhetinic acid, sterol fatty acid ester, higher fatty acid, higher alcohol, various vegetable oils, and the like.

[0022] The complex referred to in the present invention means a mixture of a plurality of substances that exhibits one physicochemical property as a complex while maintaining the individual chemical properties of the single substances. For example, the behavior with respect to temperature may change due to complexation, or the solubility or dispersibility with respect to a solvent may change.

[0023] The method for producing the composite of the present invention is as follows. First, all components to be complexed are uniformly dissolved or dispersed in an organic solvent. At this time, it can be carried out efficiently by using means such as heating and stirring. Next, the organic solvent is removed from the organic solvent solution, and all components to be complexed are simultaneously precipitated. As this method, for example, a method of distilling off the organic solvent by heating or / and under reduced pressure the organic solvent solution, a method of spray-drying the organic solvent solution, a method of instantaneously freezing the organic solvent with liquid nitrogen or the like and then freeze-drying it, etc. can be mentioned. By such a method, the target composite can be obtained in a semi-solid, solid, or powder state, but it is preferably obtained in a powder state from the viewpoint of the swelling rate when dispersed in water. From this, it is preferable to use spray-drying and freeze-drying as the production method. Further, as spray-drying, the organic solvent solution is supplied to a tubular heater at a constant rate, heated in the heater to evaporate the organic solvent to form a mixture of substantially solid content and organic solvent vapor, and this mixture is introduced into a vacuum chamber at high speed, and it is most preferable to use a device (instantaneous vacuum dryer) that instantaneously volatilizes the organic solvent. Details of this device are described in Patent Document 4.

[0024] The organic solvent used in the production of the composite of the present invention is not particularly limited as long as it can uniformly dissolve or disperse all components to be complexed. For example, hydrocarbons such as pentane, hexane, heptane, and cyclohexane; halogenated hydrocarbons such as methylene chloride and chloroform; aromatic hydrocarbons such as benzene and toluene; lower alcohols such as methanol, ethanol, isopropanol, and t-butyl alcohol; esters such as methyl acetate and ethyl acetate can be mentioned. These organic solvents may be used alone or may be used as a mixture of two or more. Among these, from the viewpoints of solubility and safety, etc., hydrocarbons such as pentane, hexane, heptane, and cyclohexane, and lower alcohols such as methanol, ethanol, isopropanol, and t-butyl alcohol can be mentioned as preferable ones.

[0025] The amount of the organic solvent used in the production of the composite of the present invention is not particularly limited as long as all the components to be complexed are uniformly dissolved or dispersed, but the amount can be appropriately changed according to the solubility of the components to be complexed. Generally, it is preferably used in an amount of 1 to 100 times by mass, more preferably 3 to 50 times by mass, based on all the components to be complexed.

[0026] As described above, the composite of the present invention obtained in this way can obtain positively charged liposomes by performing a simple operation of dispersing in water because each component is complexed. If the components contained in the composite of the present invention are simply mixed without being complexed, positively charged liposomes are not formed even when dispersed in water, or if formed, they contain insoluble substances or have poor dispersion stability.

[0027] Liposomes can be prepared from the complex of the present invention by adding the complex directly to water and stirring and mixing. Alternatively, liposomes can be prepared by first dissolving or dispersing the complex in a polyhydric alcohol, and then adding water to the resulting polyhydric alcohol solution while stirring, or by adding a polyhydric alcohol solution to water while stirring. Even in these cases, the complex of the present invention is advantageous in that its solubility and dispersibility in polyhydric alcohol are improved by its complexation. When prepared using a polyhydric alcohol, the resulting liposome solution has a more transparent appearance and better dispersion stability. Specific examples of polyhydric alcohols that can dissolve or disperse the complex of the present invention include dihydric alcohols such as ethylene glycol, propylene glycol, 1,3-propanediol, 1,2-butylene glycol, 1,3-butylene glycol, 2,3-butylene glycol, tetramethylene glycol, hexylene glycol, octylene glycol, 1,2-pentanediol, 1,2-hexanediol, 1,2-heptanediol, 1,2-octanediol, and 1,2-decanediol; and trihydric alcohols such as glycerin, trimethylolpropane, and 1,2,6-hexanetriol. Examples of suitable surfactants include sugar alcohols such as sorbitol, xylitol, erythritol, maltitol, and mannitol; glyceryl ethers such as ethylglycerin, butylglycerin, hexylglycerin, ethylhexylglycerin, and cyclohexylglycerin; and polyhydric alcohol polymers such as diethylene glycol, triethylene glycol, tetraethylene glycol, polyethylene glycol, dipropylene glycol, tripropylene glycol, polypropylene glycol, diglycerin, triglycerin, tetraglycerin, polyglycerin, isosorbide, and derivatives thereof. These may be used alone or in combination of two or more. Among these, glycerin, diglycerin, propylene glycol, 1,3-propanediol, dipropylene glycol, 1,3-butylene glycol, 1,2-pentanediol, 1,2-hexanediol, 1,2-heptanediol, and 1,2-octanediol are preferred from the viewpoints of the transparency and dispersion stability of the resulting liposome solution.

[0028] There are no particular restrictions on the stirring method when dispersing the complex of the present invention in water, and it is advisable to disperse it using a commonly used stirring device. Further, the obtained liposomes may be sized by high-pressure treatment using a microfluidizer or the like or sizing such as sizing by an extruder.

[0029] The liposomes prepared from the complex of the present invention have a positively charged surface, and thus are excellent in the permeability of the active ingredient into the skin. Therefore, the liposomes prepared from the complex of the present invention may contain an active ingredient other than the essential components of the present invention as long as the effects of the present invention are not impaired. Further, as described above, the component (B) of the present invention positively charges the liposome surface and also acts as an active ingredient. Therefore, the liposomes prepared from the complex of the present invention have enhanced permeability of the component (B) into the skin. Further, since it is known that hair also has a negatively charged surface, the liposomes prepared from the complex of the present invention are considered to be excellent in the permeability of the active ingredient to hair.

[0030] The active ingredient that can be contained in the liposome prepared by the complex of the present invention may be either a lipophilic or hydrophilic active ingredient. Examples of lipophilic active ingredients include ceramides such as ceramide 1, ceramide 2, ceramide 3, ceramide 4, ceramide 5, ceramide 6, and ceramide 7; carotenoids such as carotene, lycopene, cryptosanthin, lutein, zeaxanthin, astaxanthin, crocetin, and fucoxanthin; α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, α-tocotrienol, β-tocotrienol, and α-tocotrienol. Examples of the active ingredient include tocopherols such as γ-tocotrienol, δ-tocotrienol, tocopherol acetate, and tocopherol nicotinate; retinols such as retinol, retinol acetate, retinol palmitate, retinoic acid, tocopheryl retinoate, and retinal; lipoic acid, coenzyme Q10, ferulic acid, ursolic acid, glycyrrhetinic acid, sterol fatty acid esters, higher fatty acids, higher alcohols, and various vegetable oils.Examples of hydrophilic active ingredients include hydroquinone glycosides such as β-arbutin and α-arbutin and their esters; ascorbic acid phosphate ester salts such as ascorbic acid, sodium ascorbate phosphate, and magnesium ascorbate phosphate; ascorbic acid fatty acid esters such as ascorbic acid monostearate, ascorbic acid monopalmitate, ascorbic acid dipalmitate, and ascorbic acid tetraisopalmitate; ascorbic acid ethers such as 3-O-ethylascorbic acid, 2-O-ethylascorbic acid, cetyl ascorbic acid, glyceryl ascorbic acid, and hexyl glyceryl ascorbic acid; ascorbic acid glucosides such as ascorbic acid-2-glucoside and their fatty acid esters; ascorbic acid derivatives such as ascorbic acid sulfate ester and tocopheryl ascorbyl phosphate; tranexamic acid derivatives such as tranexamic acid, cetyl tranexamate, and tranexamic acid amide; polyphenols such as phlorotannin, curcumin, anthocyanin, proanthocyanin, catechin, ellagic acid, and apple polyphenol; nicotinamide, kojic acid, 4-methoxysalicylic acid, placenta extract, glutathione, hyaluronic acid, various plant extracts, and the like.

[0031] As a method for incorporating an active ingredient into liposomes prepared from the complex of the present invention, when the active ingredient is oil-soluble, it is advisable to add the oil-soluble active ingredient to a polyhydric alcohol solution obtained by diluting the complex of the present invention with a polyhydric alcohol and then disperse it in water. When the active ingredient is water-soluble, it is advisable to add and disperse the complex of the present invention or a polyhydric alcohol solution obtained by dissolving or dispersing the complex of the present invention in a polyhydric alcohol in water in which the water-soluble active ingredient has been previously dissolved.

[0032] When the liposomes prepared by the complex of the present invention are applied to the skin, they have an excellent feel in use. Generally, liposomes are known to have an excellent feel in use. Surprisingly, however, the liposomes prepared by the complex of the present invention are superior in feel in use, such as ease of spreading during application, lack of stickiness during drying, and moist feeling after drying, compared to liposomes that do not contain component (B) of the present invention.

[0033] The liposomes prepared by the complex of the present invention are obtained in a form dispersed in water (liposome solution). The liposome solution prepared by the complex of the present invention can be used as it is as a cosmetic or a topical skin preparation, or can be used by being incorporated as a raw material during the production of a cosmetic or a topical skin preparation.

[0034] In the present invention, that the surface is positively charged means that when the zeta potential of the liposome solution is measured by the electrophoretic light scattering measurement method, the measured value is positive (plus). Generally, the larger the absolute value of the zeta potential, the greater the electrostatic repulsive force between particles, so the dispersion stability of the particles in water is improved. From such a viewpoint, the zeta potential of the liposomes prepared by the complex of the present invention is preferably 5 mV or more, more preferably 10 mV or more, and still more preferably 15 mV or more at 25°C.

[0035] The pH of the liposome solution prepared by the complex of the present invention is preferably 3 or more, more preferably 3.5 or more, and still more preferably 4 or more at 25°C. When the pH is lower than this, it is not preferable from the viewpoint of irritation to the skin. On the other hand, the pH of the liposome solution is preferably 9 or less, more preferably 8.5 or less, and still more preferably 8 or less at 25°C. When the pH is higher than this, the liposome surface may not be sufficiently positively charged.

[0036] In the present invention, for the preparation of the pH of the liposome solution, pH adjusters generally used in cosmetics or external skin preparations can be used. Specifically, as acidic components, inorganic acids such as hydrochloric acid, sulfuric acid, and phosphoric acid; organic acids such as acetic acid, oxalic acid, malonic acid, succinic acid, glutaric acid, lactic acid, malic acid, tartaric acid, citric acid, gluconic acid, glutamic acid, aspartic acid, and phytic acid can be mentioned. Among these, succinic acid, lactic acid, malic acid, and citric acid are preferred, and lactic acid is most preferred. Further, as basic components, inorganic bases such as potassium hydroxide, sodium hydroxide, potassium carbonate, and sodium carbonate; organic bases such as ammonium, monoethanolamine, diethanolamine, triethanolamine, aminomethylpropanol, aminomethylpropanediol, aminoethylpropanediol, tris(hydroxymethyl)aminoethane, lysine, and arginine can be mentioned. Among these, potassium hydroxide, sodium hydroxide, potassium carbonate, sodium carbonate, triethanolamine, and arginine are preferred, and potassium hydroxide and sodium hydroxide are most preferred.

[0037] For the liposome solution prepared by the complex of the present invention, or for cosmetics or external skin preparations, a thickener can be blended for the purpose of improving the dispersion stability of the liposomes or imparting a desired feeling of use. Considering that the surface of the liposomes of the present invention is positively charged, it is preferable to use a nonionic polymer or a cationic polymer as the thickener that can be used in the present invention. Specific examples of the nonionic polymer that can be used in the present invention include hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropylmethylcellulose, stearoxyhydroxypropylmethylcellulose, pullulan, roasted bean gum, guar gum, tamarind seed gum, glucomannan, N-acetylglucosamine, collagen, polyvinylpyrrolidone, polyvinylpyrrolidone / vinyl acetate copolymer, polyvinyl alcohol, polyacrylamide, (PEG-240 / decyltetradeceth-20 / HDI) copolymer, and the like.Examples of the cationic polymer include chitosan, cationized cellulose, cationized guar gum, cationized xanthan gum, cationized starch, cationized fenugreek gum, cationized tara gum, cationized locust bean gum, diallyldimethylammonium chloride polymer, diallyldimethylammonium chloride-acrylamide copolymer, acrylic acid-diallyldimethylammonium chloride copolymer, acrylic acid-diallyldimethylammonium chloride-acrylamide copolymer, acrylic acid-methacryloyloxyethyltrimethylammonium chloride copolymer, acrylamide-methacryloyloxyethyltrimethylammonium chloride copolymer, acrylamide-acryloyloxyethyltrimethylammonium chloride copolymer, acrylic acid-methyl acrylate-methacrylamidopropyltrimethylammonium chloride copolymer, acrylamide-acrylic acid-methacrylamidopropyltrimethylammonium chloride copolymer, vinylpyrrolidone-methacrylamidopropyltrimethylammonium chloride copolymer, methacrylamidopropylammonium chloride-dimethyldiallylammonium chloride-acrylamide copolymer, vinylpyrrolidone-dimethylaminoethyl methacrylate copolymer diethyl sulfate, cationic methacryloyloxyethyl phosphorylcholine copolymer, and other (meth)acrylic acid derivatives.

[0038] On the other hand, considering that the surface of the liposome of the present invention is positively charged, using an anionic polymer as a thickener is generally considered unfavorable because it may cause aggregation and precipitation of the liposome of the present invention. However, although the transparency of the appearance decreases, there are some anionic polymers that do not cause aggregation and precipitation and can be used as thickeners. Specific examples of the anionic polymer that can be used as a thickener include (co)polymers containing (meth)acrylic acid and / or (meth)acryloyldimethyltaurine as monomer units, such as carboxyvinyl polymer, (acrylates / alkyl acrylate (C10-30)) cross-polymer, (acrylic acid / lauryl methacrylate / isodecyl methacrylate) cross-polymer, (sodium acrylate / sodium acryloyldimethyltaurine) copolymer, (ammonium acryloyldimethyltaurine / VP) copolymer, (acrylamide / sodium acryloyldimethyltaurine) cross-polymer, etc.; phosphorylated cellulose nanofibers, propylene glycol alginate, hyaluronic acid, and the like. In addition, anionic polymers such as xanthan gum, gellan gum, agar, silkworm polysaccharide, and carboxymethylated cellulose nanofibers cause aggregation and precipitation of the liposome of the present invention. However, even such an anionic polymer that causes aggregation and precipitation can be formulated without causing aggregation and precipitation by adding it after adding an anionic polymer that does not cause the above-mentioned aggregation and precipitation.

[0039] The cosmetics or external skin preparations containing the complex of the present invention may contain, if necessary, water and additive components usually formulated in cosmetics, such as oily bases, humectants / slipperiness improvers, surfactants, polymers / thickeners / gelling agents, antioxidants, reducing agents, oxidizing agents, anti-oxidants, skin brightening agents, vitamins and their derivatives, extracts of plants / animals / microorganisms, ultraviolet absorbers, antibacterial / antiseptic agents, chelating agents, pH adjusters / acids / alkalis, solvents / propellants, antipruritics, keratolytic / dissolving agents, antiperspirants, cooling agents, astringents, enzymes, nucleic acids, anti-inflammatory agents, hair growth agents / blood circulation promoters / stimulants, hormones, anti-wrinkle / anti-aging agents, irritation relievers, cooling sensation agents, warming sensation agents, powders, pigments / coloring agents / dyes / pigments, fragrances, etc., as long as the effects of the present invention are not impaired.

[0040] As the oily base, higher alcohols such as cetyl alcohol, myristyl alcohol, oleyl alcohol, lauryl alcohol, cetostearyl alcohol, stearyl alcohol, arachyl alcohol, behenyl alcohol, jojoba alcohol, chimyl alcohol, ceraryl alcohol, batyl alcohol, hexyl decanol, isostearyl alcohol, 2-octyldodecanol, dimer diol, etc.; aralkyl alcohols such as benzyl alcohol and derivatives; higher fatty acids such as lauric acid, myristic acid, palmitic acid, stearic acid, isostearic acid, behenic acid, undecylenic acid, 12-hydroxystearic acid, palmitoleic acid, oleic acid, linoleic acid, linolenic acid, erucic acid, docosahexaenoic acid, eicosapentaenoic acid, isohexadecanoic acid, anteisoheneicosanoic acid, long-chain branched fatty acids, long-chain α-hydroxy fatty acids, dimer acid, hydrogenated dimer acid, etc. and their aluminum salts, calcium salts, magnesium salts, zinc salts, potassium, sodium salts, etc. of metal soaps, and nitrogen-containing derivatives such as amides; hydrocarbons such as liquid paraffin (mineral oil), heavy liquid isoparaffin, light liquid isoparaffin, α-olefin oligomer, polyisobutene, hydrogenated polyisobutene, polybutene, squalane, olive-derived squalane, squalene, petrolatum, solid paraffin, etc.; waxes such as candelilla wax, carnauba wax, rice wax, wood wax, beeswax, montan wax, ozokerite, ceresin, paraffin wax, microcrystalline wax, petrolatum, Fischer-Tropsch wax, polyethylene wax, ethylene-propylene copolymer, etc.; vegetable oils and fats such as coconut oil, palm oil, palm kernel oil, safflower oil, olive oil, castor oil, avocado oil, sesame oil, tea oil, evening primrose oil, wheat germ oil, macadamia nut oil, hazelnut oil, kukui nut oil, rose hip oil, meadowfoam oil, persic oil, tea tree oil, peppermint oil, corn oil, rapeseed oil, sunflower oil, wheat germ oil, linseed oil, cottonseed oil, soybean oil, peanut oil, rice bran oil, cocoa butter, shea butter, hydrogenated coconut oil, hydrogenated castor oil, jojoba oil, hydrogenated jojoba oil, etc.; animal fats and oils such as beef tallow, butter, horse fat, egg yolk oil, mink oil, turtle oil, etc.; animal waxes such as spermaceti, lanolin, orange raffia oil, etc.;Lanolin derivatives such as liquid lanolin, reduced lanolin, adsorption-purified lanolin, lanolin acetate, liquid lanolin acetate, hydroxy lanolin, polyoxyethylene lanolin, lanolin fatty acid, hard lanolin fatty acid, lanolin alcohol, lanolin alcohol acetate, and acetate (cetyl lanolyl) ester; Sphingophospholipids such as lecithin, phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and sphingomyelin; Phospholipids such as phosphatidic acid, cyclic lysophosphatidic acid or its salts, and lysophosphatidylcholine; Phospholipid derivatives such as hydrogenated soybean phospholipid, partially hydrogenated soybean phospholipid, hydrogenated egg yolk phospholipid, and partially hydrogenated egg yolk phospholipid; Sterols such as cholesterol, dihydrocholesterol, lanosterol, dihydrolanosterol, phytosterol, and cholic acid; Saponins; Sapogenins; Acyl sarcosine alkyl esters such as cholesteryl acetate, cholesteryl nonanoate, cholesteryl stearate, cholesteryl isostearate, cholesteryl oleate, N-lauroyl-L-glutamic acid di (cholesteryl / behenyl / octyldodecyl), N-lauroyl-L-glutamic acid di (cholesteryl / octyldodecyl), N-lauroyl-L-glutamic acid di (phytosteryl / behenyl / octyldodecyl), N-lauroyl-L-glutamic acid di (phytosteryl / octyldodecyl), and N-lauroyl sarcosine isopropyl; Sterol esters such as cholesteryl 12-hydroxystearate, cholesteryl macadamia nut oil fatty acid, phytosteryl macadamia nut oil fatty acid, phytosteryl sunflower seed oil fatty acid, phytosteryl isostearate, cholesteryl soft lanolin fatty acid, cholesteryl hard lanolin fatty acid, cholesteryl long-chain branched fatty acid, and cholesteryl long-chain α-hydroxy fatty acid; Lipid complexes such as phospholipid-cholesterol complex and phospholipid-phytosterol complex;Octyldodecyl myristate, hexyl decyl myristate, octyldodecyl isostearate, cetyl palmitate, octyldodecyl palmitate, cetyl octanoate, hexyl decyl octanoate, isotridecyl isononanoate, isononyl isononanoate, octyl isononanoate, isotridecyl isononanoate, isodecyl neopentanoate, isotridecyl neopentanoate, isostearyl neopentanoate, octyldodecyl neodecanoate, oleyl oleate, octyldodecyl oleate, octyldodecyl ricinoleate, octyldodecyl lanolin fatty acid, dicaprylyl carbonate, hexyl decyl dimethyl octanoate, octyldodecyl erucate, hydrogenated castor oil isostearate, ethyl oleate, ethyl avocado oil fatty acid, isopropyl myristate, isopropyl palmitate, octyl palmitate, isopropyl isostearate, isopropyl lanolin fatty acid, methyl heptyl laurate, methyl heptyl myristate, methyl heptyl palmitate, methyl heptyl isostearate, diethyl sebacate, diisopropyl sebacate, dioctyl sebacate, diisopropyl adipate, dibutyloctyl sebacate, diisobutyl adipate, dioctyl succinate, triethyl citrate and other monoalcohol carboxylic acid esters; oxyacid esters such as cetyl lactate, hydrogenated castor oil monoisostearate, γ-elaeolactone, diisostearyl malate;Glyceryl trioctanoate, glyceryl trioleate, glyceryl triisostearate, glyceryl diisostearate, glyceryl tri(caprylic / capric acid), glyceryl tri(caprylic / capric / myristic / stearic acid), hydrogenated rosin triglyceride (hydrogenated ester gum), rosin triglyceride (ester gum), glyceryl behenate eicosanedioate, trimethylolpropane trioctanoate, trimethylolpropane triisostearate, neopentyl glycol dioctanoate, neopentyl glycol dicaprate, 2-butyl-2-ethyl-1,3-propanediol dioctanoate, propylene glycol dioleate, pentaerythrityl tetraoctanoate, hydrogenated rosin pentaerythrityl, ditrimethylolpropane triethylhexanoate, ditrimethylolpropane (isostearic / sebacic acid), pentaerythrityl triethylhexanoate, dipentaerythrityl (hydroxystearic / stearic / rosinic acid), diglyceryl diisostearate, polyglyceryl tetraisostearate, polyglyceryl-10 nonaisostearate, polyglyceryl-8 deca(erucic / isostearic / ricinoleic acid), diglyceryl oligoster ester (hexyldecanoic / sebacic acid), ethylene glycol distearate (distearic acid ethylene glycol), 3-methyl-1,5-pentanediol dineopentanoate, 2,4-diethyl-1,5-pentanediol dineopentanoate and other polyhydric alcohol fatty acid esters; alkyl ethers such as dicaprylyl ether; derivatives of dimer acid or dimer diol such as diisopropyl dimer dilinoleate, diisostearyl dimer dilinoleate, di(isostearyl / phytosteryl) dimer dilinoleate, (phytosteryl / behenyl) dimer dilinoleate, (phytosteryl / isostearyl / cetyl / stearyl / behenyl) dimer dilinoleate, dimer dilinoleyl dimer dilinoleate, diisostearyl dimer dilinoleate, hydrogenated rosin condensate of dimer dilinoleate, hydrogenated castor oil dimer dilinoleate, hydroxyalkyl dimer dilinoleyl ether, etc.Fatty acid alkanolamides such as coconut oil fatty acid monoethanolamide (cocamide MEA), coconut oil fatty acid diethanolamide (cocamide DEA), lauric acid monoethanolamide (lauramide MEA), lauric acid diethanolamide (lauramide DEA), lauric acid monoisopropanolamide (lauramide MIPA), palmitic acid monoethanolamide (palmitamide MEA), palmitic acid diethanolamide (palmitamide DEA), coconut oil fatty acid methylethanolamide (cocamide methyl MEA); Silicones such as dimethicone (dimethylpolysiloxane), highly polymerized dimethicone (highly polymerized dimethylpolysiloxane), cyclomethicone (cyclic dimethylsiloxane, decamethylcyclopentasiloxane), phenyltrimethicone, diphenyldimethylsilicone, phenyldimethylsilicone, (aminoethylaminopropylmethylsilicone / dimethylsilicone) copolymer, dimethiconol, dimethiconol cross polymer, silicone resin, silicone rubber, amino-modified silicones such as aminopropyldimethylsilicone and amodimethicone, cation-modified silicone, polyether-modified silicones such as dimethicone copolyol, polyglycerin-modified silicone, sugar-modified silicone, carboxylic acid-modified silicone, phosphoric acid-modified silicone, sulfuric acid-modified silicone, alkyl-modified silicone, fatty acid-modified silicone, alkyl ether-modified silicone, amino acid-modified silicone, peptide-modified silicone, fluorine-modified silicone, cation-modified and polyether-modified silicone, amino-modified and polyether-modified silicone, alkyl-modified and polyether-modified silicone, amidalkyl-modified silicone, aminoglycol-modified silicone, aminophenyl-modified silicone, polysiloxane-oxyalkylene copolymer; Fluorine-based oils such as perfluorodecane, perfluorooctane, perfluoropolyether are preferably mentioned.;

[0041] As the humectant and feel improver, there are polyols such as glycerin, 1,3-butylene glycol, propylene glycol, 3-methyl-1,3-butanediol, 1,3-propanediol, 2-methyl-1,3-propanediol, trimethylolpropane, pentaerythritol, hexylene glycol, diglycerin, polyglycerin, diethylene glycol, polyethylene glycol, dipropylene glycol, polypropylene glycol, ethylene glycol-propylene glycol copolymer, erythritan, and their polymers; glycol alkyl ethers such as diethylene glycol monoethyl ether (ethoxydiglycol), ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, diethylene glycol dibutyl ether; water-soluble esters such as (eicosanedioic acid / tetradecanedioic acid) polyglyceryl-10, tetradecanedioic acid polyglyceryl-10, cyclohexanedicarboxylic acid bisethoxydiglycol; sugar alcohols such as sorbitol, xylitol, erythritol, mannitol, maltitol; sugars and their derivatives such as glucose, fructose, galactose, mannose, threose, xylose, arabinose, fucose, ribose, deoxyribose, maltose, trehalose, glucosyltrehalose, lactose, raffinose, gluconic acid, glucuronic acid, cyclodextrins (α-, β-, γ-cyclodextrin, and modified cyclodextrins such as maltosylated, hydroxyalkylated cyclodextrin), β-glucan, chitin, chitosan, heparin and its derivatives, pectin, arabinogalactan, dextrin, dextran, glycogen, ethyl glucoside, glucosylethyl methacrylate polymer or copolymer; hyaluronic acid, sodium hyaluronate; sodium chondroitin sulfate; mucin sulfate, caronin sulfate, keratosulfate, dermatan sulfate; jellyfish extract, jellyfish polysaccharide; fructans such as inulin, levan; fucoidan; tuberose polysaccharide, natural-derived polysaccharide; organic acids such as citric acid, tartaric acid, lactic acid and their salts; urea; 2-pyrrolidone- carboxylic acid and its salts such as sodium salt;Amino acids such as betaine (trimethylglycine), proline, hydroxyproline, arginine, lysine, serine, glycine, alanine, phenylalanine, tyrosine, β-alanine, threonine, glutamic acid, glutamine, asparagine, aspartic acid, cysteine, cystine, methionine, leucine, isoleucine, valine, tryptophan, histidine, taurine, γ-aminobutyric acid, γ-amino-β-hydroxybutyric acid, carnitine, carnosine, creatine and their salts; protein peptides such as collagen, fish-derived collagen, atelocollagen, gelatin, elastin, collagen-degrading peptides, hydrolyzed collagen, hydroxypropylammonium chloride hydrolyzed collagen, elastin-degrading peptides, keratin-degrading peptides, hydrolyzed keratin, conchiolin-degrading peptides, hydrolyzed conchiolin, silk protein-degrading peptides, hydrolyzed silk, lauroyl hydrolyzed silk sodium, soybean protein-degrading peptides, wheat protein-degrading peptides, hydrolyzed wheat protein, casein-degrading peptides, acylated peptides and their derivatives; acylated peptides such as palmitoyl oligopeptide, palmitoyl pentapeptide, palmitoyl tetrapeptide; silylated peptides; lactic acid bacteria culture solution, yeast extract, eggshell membrane protein, bovine submandibular gland mucin, hypotaurine, sesame lignan glycoside, glutathione, albumin, whey; choline chloride, phosphorylcholine; placenta extract, aeroelastin, collagen, aloe extract, witch hazel water, loofah water, chamomile extract, licorice extract, comfrey extract, silk extract, Japanese rose extract, English plantain extract, eucalyptus extract, melilot extract and other animal and plant extract components, ceramides such as natural ceramides (type 1, 2, 3, 4, 5, 6), hydroxyceramides, pseudo-ceramides, sphingoglycolipids, ceramides and extracts containing glycosphingolipids are preferably mentioned.;

[0042] As the surfactant, anionic surfactants, nonionic surfactants, cationic surfactants, amphoteric surfactants, polymeric surfactants, etc. are preferably exemplified. There is no particular limitation on the HLB of the surfactant, and those with a low HLB of about 1 to those with a high HLB of about 20 can be used, and it is also preferable to combine those with a low HLB and those with a high HLB. Examples of preferred surfactants include, among anionic surfactants, fatty acid salts such as potassium laurate and potassium myristate; alkyl sulfate ester salts such as sodium lauryl sulfate, triethanolamine lauryl sulfate, and ammonium lauryl sulfate; polyoxyethylene alkyl sulfate salts such as sodium laureth sulfate and triethanolamine laureth sulfate; acyl N-methyl amino acid salts such as sodium cocoyl methyl taurate, potassium cocoyl methyl taurate, sodium lauroyl methyl taurate, sodium myristoyl methyl taurate, sodium lauroyl methyl alaninate, sodium lauroyl sarcosinate, triethanolamine lauroyl sarcosinate, and sodium lauroyl glutamate methyl alaninate; acyl amino acid salts such as sodium cocoyl glutamate, triethanolamine cocoyl glutamate, sodium lauroyl glutamate, sodium myristoyl glutamate, sodium stearoyl glutamate, ditriethanolamine palmitoyl aspartate, triethanolamine cocoyl alaninate, and lysine Na dilauroyl glutamate; polyoxyethylene alkyl ether acetate salts such as sodium laureth acetate; succinate ester salts such as sodium lauroyl monoethanolamide succinate; fatty acid alkanolamide ether carboxylate salts; acyl lactate salts; polyoxyethylene fatty amine sulfate salts; fatty acid alkanolamide sulfate salts; fatty acid glyceride sulfate salts such as sodium hydrogenated palm oil fatty acid glycerol sulfate; polyoxyethylene alkylbenzene sulfate salts; olefin sulfonate salts such as sodium α-olefin sulfonate; alkyl sulfosuccinate salts such as sodium lauryl sulfosuccinate and sodium dioctyl sulfosuccinate;Alkyl ether sulfosuccinates such as sodium lauryl sulfosuccinate, sodium monolaurylmonoethanolamine polyoxyethylene sulfosuccinate, sodium lauryl polypropylene glycol sulfosuccinate; alkylbenzene sulfonates such as sodium tetradecylbenzenesulfonate, triethanolamine tetradecylbenzenesulfonate; alkylnaphthalene sulfonates; alkane sulfonates; α-sulfofatty acid methyl ester salts; acyl isethionates; alkyl glycidyl ether sulfonates; alkylsulfonoacetates; alkyl ether phosphate ester salts such as sodium lauryl phosphate, sodium dilauryl phosphate, sodium trilauryl phosphate, sodium monooleyl phosphate; alkyl phosphate ester salts such as potassium lauryl phosphate; sodium caseinate; alkylaryl ether phosphates; fatty acid amide ether phosphates; phospholipids such as phosphatidylglycerol, phosphatidylinositol, phosphatidic acid, cyclic lysophosphatidic acid or its salts; silicone-based anionic surfactants such as carboxylic acid-modified silicone, phosphate-modified silicone, sulfuric acid-modified silicone, etc.; for nonionic surfactants, various polyoxyethylene adduct numbers of polyoxyethylene alkyl ethers such as laureths (polyoxyethylene lauryl ethers), cetes (polyoxyethylene cetyl ethers), stearates (polyoxyethylene stearyl ethers), behenes (polyoxyethylene behenyl ether), isostearates (polyoxyethylene isostearyl ether), octyldodeceths (polyoxyethylene octyldodecyl ether); polyoxyethylene alkyl phenyl ether; polyoxyethylene hydrogenated castor oil, polyoxyethylene castor oil, polyoxyethylene hydrogenated castor oil monoisostearate, polyoxyethylene hydrogenated castor oil triisostearate, polyoxyethylene hydrogenated castor oil monopyroglutamic acid monoisostearate diester, polyoxyethylene hydrogenated castor oil maleic acid and other castor oil and hydrogenated castor oil derivatives; polyoxyethylene phytosterol; polyoxyethylene cholesterol; polyoxyethylene cholestanol; polyoxyethylene lanolin; polyoxyethylene reduced lanolin;Polyoxyethylene-polyoxypropylene cetyl ether, polyoxyethylene-polyoxypropylene 2-decyltetradecyl ether, polyoxyethylene-polyoxypropylene monobutyl ether, polyoxyethylene-polyoxypropylene hydrogenated lanolin, polyoxyethylene-polyoxypropylene glycerin ether and other polyoxyethylene-polyoxypropylene alkyl ethers; polyoxyethylene-polyoxypropylene glycol; (poly)glycerin polyoxypropylene glycol such as PPG-9 diglyceryl; glycerin fatty acid partial esters such as glyceryl stearate, glyceryl isostearate, glyceryl palmitate, glyceryl myristate, glyceryl oleate, glyceryl coconut oil fatty acid, glycerin monoricinoleate, glyceryl sesquioleate, α,α'-oleic acid pyroglutamic acid glyceryl, glyceryl monostearate malate; polyglycerol fatty acid esters such as polyglyceryl-2 stearate, polyglyceryl-3 stearate, polyglyceryl-4 stearate, polyglyceryl-5 stearate, polyglyceryl-6 stearate, polyglyceryl-8 stearate, polyglyceryl-10 stearate, polyglyceryl-6 distearate, polyglyceryl-10 distearate, polyglyceryl-2 tristearate, polyglyceryl-10 decastearate, polyglyceryl-2 isostearate, polyglyceryl-3 isostearate, polyglyceryl-4 isostearate, polyglyceryl-5 isostearate, polyglyceryl-6 isostearate, polyglyceryl-8 isostearate, polyglyceryl-10 isostearate, polyglyceryl-2 diisostearate (diglyceryl diisostearate), polyglyceryl-3 diisostearate, polyglyceryl-10 diisostearate, polyglyceryl-2 triisostearate, polyglyceryl-2 tetraisostearate, polyglyceryl-10 decaisostearate, polyglyceryl-2 oleate, polyglyceryl-3 oleate, polyglyceryl-4 oleate, polyglyceryl-5 oleate, polyglyceryl-6 oleate, polyglyceryl-8 oleate, polyglyceryl-10 oleate, polyglyceryl-6 dioleate, polyglyceryl-2 trioleate, polyglyceryl-10 decaoleate; ethylene glycol monofatty acid esters such as ethylene glycol monostearate; propylene glycol monofatty acid esters such as propylene glycol monostearate; pentaerythritol partial fatty acid esters; sorbitol partial fatty acid esters; maltitol partial fatty acid esters; maltitol ether;Sorbitan fatty acid esters such as sorbitan monooleate, sorbitan monoisostearate, sorbitan monolaurate, sorbitan monopalmitate, sorbitan monostearate, sorbitan sesquioleate, sorbitan trioleate, diglycerol sorbitan penta-2-ethylhexanoate, diglycerol sorbitan tetra-2-ethylhexanoate; sucrose fatty acid esters such as sucrose laurate, sucrose myristate, sucrose palmitate, sucrose dilaurate, sucrose dimyristate, sucrose dipalmitate, sugar derivative partial esters such as methyl glucoside fatty acid ester, trehalose undecylenate; alkyl glucosides such as caprylyl glucoside; alkyl polyglycosides; glycolipids such as mannosyl erythritol lipid; lanolin alcohol; reduced lanolin; polyoxyethylene fatty acid mono- and diesters such as polyoxyethylene distearate, polyethylene glycol diisostearate, polyoxyethylene monooleate, polyoxyethylene dioleate; polyoxyethylene-propylene glycol fatty acid ester; polyoxyethylene glycerol fatty acid esters such as polyoxyethylene glycerol monostearate, polyoxyethylene glycerol monoisostearate, polyoxyethylene glycerol triisostearate; polyoxyethylene sorbitan fatty acid esters such as polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan monostearate, polyoxyethylene sorbitan monooleate, polyoxyethylene sorbitan tetraoleate; polyoxyethylene sorbitol fatty acid esters such as polyoxyethylene sorbitol monolaurate, polyoxyethylene sorbitol monooleate, polyoxyethylene sorbitol pentaoleate, polyoxyethylene sorbitol monostearate; polyoxyethylene methyl glucoside fatty acid ester; polyoxyethylene alkyl ether fatty acid ester; polyoxyethylene animal and vegetable oils and fats such as polyoxyethylene sorbitol beeswax; alkyl glyceryl ethers such as isostearyl glyceryl ether, chimyl alcohol, ceralkyl alcohol, batyl alcohol; polyhydric alcohol alkyl ethers;Polyoxyethylene alkylamines; tetrapolyoxyethylene-tetrapolyoxypropylene-ethylenediamine condensates; natural surfactants such as saponin, surfactin, rhamnolipid, sophorolipid, etc.; polyoxyethylene fatty acid amides; coconut oil fatty acid monoethanolamide (cocamide MEA), coconut oil fatty acid diethanolamide (cocamide DEA), lauric acid monoethanolamide (lauramide MEA), lauric acid diethanolamide (lauramide DEA), lauric acid monoisopropanolamide (lauramide MIPA), palmitic acid Fatty acid alkanolamides such as palmitic acid monoethanolamide (Palmitic acid diethanolamide (Palmitic acid DEA), coconut oil fatty acid methylethanolamide (cocamide methyl MEA); alkyl dimethylamine oxides such as lauramine oxide, cocamine oxide, stearamine oxide, and behenamine oxide; alkyl ethoxydimethylamine oxide; polyoxyethylene alkyl mercaptan; polyether-modified silicones such as dimethicone copolyol, polysiloxane-oxyalkylene copolymers, and polyglycerin-modified Silicone-based nonionic surfactants such as silicones and sugar-modified silicones; cationic surfactants include alkyltrimethylammonium chlorides such as behentrimonium chloride, steartrimonium chloride, cetrimonium chloride, and lauryltrimonium chloride; alkyltrimethylammonium bromides such as stearyltrimonium bromide; dialkyldimethylammonium chlorides such as distearyldimonium chloride and dicocodimonium chloride; fatty acid amidoamines and salts thereof such as stearamidopropyldimethylamine and stearamidoethyldiethylamine; alkyl ether amines and salts or quaternary salts thereof such as stearoxypropyldimethylamine; fatty acid amide-type quaternary ammonium salts such as ethyl sulfate of long-chain branched fatty acid (12-31) aminopropylethyldimethylammonium and ethyl sulfate of lanolin fatty acid aminopropylethyldimethylammonium; polyoxyethylene alkylamines and salts or quaternary salts thereof; alkylamine salts; fatty acid amide guanidinium salts; alkyl ether amine ammonium salts; alkyl trialkylene glycol ammonium salts; benzalkonium salts;Benzethonium salts; pyridinium salts such as cetylpyridinium chloride; imidazolinium salts; alkylisoquinolinium salts; dialkylmorphonium salts; polyamine fatty acid derivatives; amino-modified silicones such as aminopropyldimethylsilicone and amodimethicone, cation-modified silicones, cation-modified and polyether-modified silicones, amino-modified and polyether-modified silicones and other silicone-based cationic surfactants; for amphoteric surfactants, N-alkyl-N,N-dimethylamino acid betaines such as lauryl betaine (lauryldimethylaminoacetic acid betaine); fatty acid amide alkyl-N,N-dimethylamino acid betaines such as cocoamidopropyl betaine and lauramidopropyl betaine; imidazoline-type betaines such as sodium cocoamphoacetate and sodium lauroamphoacetate; alkylsulfobetaines such as alkyldimethyltaurine; sulfate-type betaines such as alkyldimethylaminoethanol sulfate ester; phosphate-type betaines such as alkyldimethylaminoethanol phosphate ester; sphingolipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, sphingomyelin, lysophosphatidylcholine, hydrogenated soybean phospholipid, partially hydrogenated soybean phospholipid, hydrogenated egg yolk phospholipid, partially hydrogenated egg yolk phospholipid, hydroxylated phospholipids and other phospholipids; silicone-based amphoteric surfactants; for polymeric surfactants, polyvinyl alcohol, sodium alginate, starch derivatives, tragacanth gum, acrylic acid·methacrylic acid alkyl copolymers; various silicone-based surfactants are preferably mentioned.

[0043] Examples of polymers, thickeners, and gelling agents include guar gum, locust bean gum, carob seed, carrageenan, galactan, gum arabic, tara gum, tamarind, farselan, karaya gum, trollius, carrageenan, tragacanth gum, pectin, pectic acid and its salts such as sodium salt, alginic acid and its salts such as sodium salt, mannan; starches such as rice, corn, potato, and wheat; xanthan gum, dextran, succinoglucan, curdlan, hyaluronic acid and its salts, xanthan gum, pullulan, gellan gum, chitin, chitosan, agar, cassowary extract, chondroitin sulfate, casein, collagen, gelatin, albumin; methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose and its salts such as sodium salt, methylhydroxypropylcellulose, carboxylated cellulose nanofiber, carboxymethylated cellulose nanofiber, phosphate-esterified cellulose nanofiber, sulfonated cellulose nanofiber, sodium cellulose sulfate, dialkyldimethylammonium sulfate cellulose, crystalline cellulose, cellulose powder and other cellulose and its derivatives; starch-based polymers such as soluble starch, carboxymethyl starch, methylhydroxypropyl starch, methyl starch, hydroxypropyltrimonium chloride starch, octenyl succinic anhydride corn starch aluminum and other starch derivatives; alginic acid derivatives such as sodium alginate, propylene glycol alginate; polyvinyl pyridone (PVP), polyvinyl alcohol (PVA), vinyl pyridone-vinyl alcohol copolymer, polyvinyl methyl ether; polyethylene glycol, polypropylene glycol, polyoxyethylene-polyoxypropylene copolymer; amphoteric methacrylic acid ester copolymers such as (methacryloyloxyethyl carboxybetaine / methacrylic acid alkyl) copolymer, (acrylates / stearyl acrylate / ethylamine oxide methacrylate) copolymer; (dimethyl silicone / vinyl dimethyl silicone) cross-polymer, (alkyl acrylate / diacetone acrylamide) copolymer, (alkyl acrylate / diacetone acrylamide) copolymer AMP;Partially saponified polyvinyl acetate, maleic acid copolymer; vinyl pyrrolidone-dialkylaminoalkyl methacrylate copolymer; acrylic resin alkanolamine; polyester, water-dispersible polyester; polyacrylamide; polyacrylate esters copolymer such as polyethyl acrylate, carboxyvinyl polymer, polyacrylic acid and its salts such as sodium salt, acrylic acid-methacrylic acid ester copolymer; acrylic acid-alkyl methacrylate copolymer; cationized cellulose such as polyquaternium-10, diallyldimethylammonium chloride-acrylamide copolymer such as polyquaternium-7, acrylic acid-diallyldimethylammonium chloride copolymer such as polyquaternium-22, acrylic acid-diallyldimethylammonium chloride-acrylamide copolymer such as polyquaternium-39, acrylic acid-cationized methacrylic acid ester copolymer, acrylic acid-cationized methacrylamide copolymer, acrylic acid-methyl acrylate-methacrylamidopropyltrimethylammonium chloride copolymer such as polyquaternium-47, methacrylic acid choline ester polymer; cationized oligosaccharide, cationized dextran, cationized polysaccharides such as guar hydroxypropyltrimonium chloride; polyethyleneimine; cationic polymer; polymer of 2-methacryloyloxyethyl phosphorylcholine such as polyquaternium-51 and copolymer with butyl methacrylate etc.; acrylic resin emulsion, polyethyl acrylate emulsion, polyalkyl acrylate emulsion, polyvinyl acetate resin emulsion, natural rubber latex, synthetic latex and other polymer emulsions; nitrocellulose; polyurethanes and various copolymers; various silicones; various silicone copolymers such as acrylic-silicone graft copolymer; various fluorine-based polymers; 12-hydroxystearic acid and its salts; dextrin fatty acid esters such as dextrin palmitate, dextrin myristate;Anhydrous silicic acid, fumed silica (ultrafine anhydrous silicic acid), magnesium aluminum silicate, magnesium sodium silicate, metal soap, metal dialkyl phosphate, bentonite, hectorite, organically modified clay minerals, sucrose fatty acid ester, fructooligosaccharide fatty acid ester are preferably mentioned.;

[0044] Examples of the antioxidant include tocopherol (vitamin E), tocopherol derivatives such as tocopherol acetate; BHT, BHA; gallic acid derivatives such as propyl gallate; vitamin C (ascorbic acid) and / or its derivatives; erythorbic acid and its derivatives; sulfites such as sodium sulfite; bisulfites such as sodium bisulfite; thiosulfates such as sodium thiosulfate; metabisulfites; thiotaurine, hypotaurine; thioglycerol, thiourea, thioglycolic acid, cysteine hydrochloride. Examples of the reducing agent include thioglycolic acid, cysteine, cysteamine and the like. Examples of the oxidizing agent include hydrogen peroxide solution, ammonium persulfate, sodium bromate, percarbonate and the like. Examples of the antioxidant include ascorbic acid phosphate ester salts such as ascorbic acid, sodium ascorbic acid phosphate ester and magnesium ascorbic acid phosphate ester; ascorbic acid fatty acid esters such as ascorbic acid monostearate, ascorbic acid monopalmitate, ascorbic acid dipalmitate, ascorbic acid tetraisopalmitate; ascorbic acid ethers such as 3-O-ethyl ascorbic acid, 2-O-ethyl ascorbic acid, cetyl ascorbic acid, glyceryl ascorbic acid, hexyl glyceryl ascorbic acid; ascorbic acid glucosides such as ascorbic acid-2-glucoside and its fatty acid esters; ascorbic acid derivatives such as ascorbic acid sulfate ester, tocopheryl ascorbyl phosphate; vitamin A compounds such as retinol, retinol acetate, retinol palmitate, hydrogenated retinol; vitamin E compounds such as tocopherol, tocopherol acetate, tocotrienol; carotenoids such as carotene, lycopene, astaxanthin, lutein; polyphenols such as phlorotannin, curcumin, anthocyanin, proanthocyanidin, catechin, ellagic acid, quercetin, apple polyphenol; coenzyme Q10, lipoic acid, lactoferrin, sesamin, hesperidin, proanthocyanidin, lignan, chlorogenic acid, rutin, fullerene, platinum nanocolloid and the like.

[0045] Examples of skin-whitening agents include hydroquinone glycosides such as arbutin and α-arbutin and their esters; ascorbic acid phosphate ester salts such as ascorbic acid, sodium ascorbic acid phosphate ester, and magnesium ascorbic acid phosphate ester; ascorbic acid fatty acid esters such as ascorbic acid monostearate, ascorbic acid monopalmitate, ascorbic acid dipalmitate, and ascorbic acid tetraisopalmitate; ascorbic acid ethers such as 3-O-ethyl ascorbic acid, 2-O-ethyl ascorbic acid, cetyl ascorbic acid, glyceryl ascorbic acid, and hexyl glyceryl ascorbic acid; ascorbic acid glucosides such as ascorbic acid-2-glucoside and their fatty acid esters; ascorbic acid derivatives such as ascorbic acid sulfate ester and tocopheryl ascorbyl phosphate; tranexamic acid derivatives such as tranexamic acid, cetyl tranexamic acid, and tranexamic acid amide; licorice-related substances such as glabridin, glabrene, liquiritin, and isoliquiritin; plant extracts such as kojic acid, ellagic acid, ferulic acid and its derivatives, placenta extract, glutathione, oryzanol, butylresorcinol, oil-soluble chamomile extract, oil-soluble licorice extract, tamarix chinensis extract, and saxifraga stolonifera extract are preferably mentioned.

[0046] Examples of vitamins and their derivatives include vitamin A compounds such as retinol, retinol acetate, and retinol palmitate; vitamin B group compounds such as thiamine hydrochloride, thiamine sulfate, riboflavin, riboflavin acetate, pyridoxine hydrochloride, pyridoxine dioctanoate, pyridoxine dipalmitate, flavin adenine dinucleotide, cyanocobalamin, folic acids, nicotinic acid compounds such as nicotinamide and benzyl nicotinate, and choline compounds; vitamin C compounds such as ascorbic acid and its salts such as sodium ascorbate; vitamin D; vitamin E compounds such as α, β, γ, δ-tocopherol; other vitamins such as pantothenic acid and biotin; ascorbic acid phosphate salts such as sodium ascorbic acid phosphate and magnesium ascorbic acid phosphate, ascorbic acid fatty acid esters such as ascorbic acid tetraisopalmitate, stearyl ascorbate, palmitoyl ascorbate, and dipalmitoyl ascorbate, ascorbic acid alkyl ethers such as ascorbic acid ethyl ether, ascorbic acid glucosides such as ascorbic acid-2-glucoside and their fatty acid esters, ascorbic acid derivatives such as ascorbyl phosphate tocopherol; vitamin derivatives such as tocopherol derivatives such as tocopherol nicotinate, tocopherol acetate, tocopherol linoleate, tocopherol ferulate, and tocopherol phosphate ester, tocotrienol, and other various vitamin derivatives are preferably mentioned.

[0047] Examples of plant, animal, and microbial extracts include iris extract, ashitaba extract, asnaro extract, asparagus extract, avocado extract, amacha extract, almond extract, hollyhock extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo extract, magnolia bark extract, turmeric extract, saffron extract, oolong tea extract, Japanese lacquer extract, euryale extract, etinashi leaf extract, polygonatum extract, gold extract, cinnamon extract, ginseng extract, oil-soluble ginseng extract, kiwi extract, dioscorea extract, shiitake mushroom extract, cinchona extract, cucumber extract, mulberry leaf extract, guanosine, guava extract, kudzu extract, gardenia extract, kumasasa extract, clara extract, walnut extract, chestnut extract, grapefruit extract, clematis extract, black rice extract, black sugar extract, black vinegar extract, chlorella extract, mulberry extract, gentiana extract, licorice extract, black tea extract, yeast extract, kowboku extract, coffee extract, burdock extract, rice extract, fermented rice extract, rice bran fermented extract, rice germ oil, comfrey extract, collagen, loquat extract, saishin extract, saiko extract, saibitai extract, saffron extract, sage extract, soapwort extract, sasae extract, Japanese quince extract, sanshya extract, sansho extract, shiitake mushroom extract, ginger extract, shikon extract, perilla extract, sinano oak extract, shimotsuke sorrel extract, jatoba extract, peony extract, shoukyu extract, calamus root extract, silver birch extract, white shiitake mushroom extract, sugina extract, stevia extract, fermented stevia, tamarix extract, European ivy extract, European quince extract, European nard extract, European speedwell extract, European mint extract, sage extract, zeniao extract, centaury extract, senburi extract, souhakuhi extract, daiou extractExtracts such as soybean extract, spinach extract, thyme extract, dandelion extract, lichen extract, tea extract, clove extract, chigaia extract, chinpi extract, tea tree oil, sweet tea extract, pepper extract, toki extract, toki nsenka extract, tou nin extract, thuja extract, dokudami extract, tomato extract, natto extract, carrot extract, garlic extract, rosa multiflora extract, hibiscus extract, buckwheat extract, lotus extract, parsley extract, birch extract, honey, witch hazel extract, Parietaria extract, hikiokoshi extract, bisabolol, hinoki extract, bifidobacterium extract, loquat extract, fuki dandelion extract, fiddlehead fern extract, bukuryou extract, butcher's bloom extract, grape extract, grape seed extract, propolis, loofah extract, safflower extract, peppermint extract, bodaiju extract, button extract, hop extract, maikaika extract, pine extract, plane tree extract, mizubashou extract, mukuroji extract, melissa extract, mozuku extract, peach extract, yaguruma giku extract, eucalyptus extract, snowdrop extract, yuzu extract, lily extract, yokuin nin extract, mugwort extract, lavender extract, green tea extract, eggshell membrane extract, apple extract, rooibos tea extract, raisin extract, lettuce extract, lemon extract, kelp extract, renge sou extract, rose extract, rosemary extract, roman chamomile extract, royal jelly extract, waremokou extract, etc. are preferably mentioned.

[0048] Examples of ultraviolet absorbers include benzoic acid-based ultraviolet absorbers such as para-aminobenzoic acid, monoglycerin ester of para-aminobenzoic acid, ethyl N,N-dipropoxy para-aminobenzoate, ethyl N,N-diethoxy para-aminobenzoate, ethyl N,N-dimethyl para-aminobenzoate, butyl N,N-dimethyl para-aminobenzoate, ethyl N,N-dimethyl para-aminobenzoate; anthranilic acid-based ultraviolet absorbers such as homomenthyl-N-acetylanthranilate; salicylic acid and its sodium salt, amyl salicylate, menthyl salicylate, homomenthyl salicylate, octyl salicylate, phenyl salicylate, benzyl salicylate, p-isopropanolphenyl salicylate and other salicylic acid-based ultraviolet absorbers; cinnamic acid-based ultraviolet absorbers such as octyl cinnamate, ethyl 4-isopropyl cinnamate, methyl 2,5-diisopropyl cinnamate, ethyl 2,4-diisopropyl cinnamate, methyl 2,4-diisopropyl cinnamate, propyl p-methoxy cinnamate, isopropyl p-methoxy cinnamate, isoamyl p-methoxy cinnamate, 2-ethylhexyl p-methoxy cinnamate (octyl paramethoxycinnamate), 2-ethoxyethyl-p-methoxy cinnamate (sinoxate), cyclohexyl-p-methoxy cinnamate, ethyl α-cyano-β-phenyl cinnamate, 2-ethylhexyl α-cyano-β-phenyl cinnamate (octocrylene), glyceryl mono-2-ethylhexanoyl-diparamethoxy cinnamate, ferulic acid and its derivatives.Benzophenone-based UV absorbers such as 2,4-dihydroxybenzophenone, 2,2'-dihydroxy-4-methoxybenzophenone, 2,2'-dihydroxy-4,4'-dimethoxybenzophenone, 2,2',4,4'-tetrahydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone (oxybenzone-3), 2-hydroxy-4-methoxy-4'-methylbenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-phenylbenzophenone, 2-ethylhexyl-4'-phenyl-benzophenone-2-carboxylate, 2-hydroxy-4-n-octoxybenzophenone, 4-hydroxy-3-carboxybenzophenone; 3-(4'-methylbenzylidene)-d,l-camphor, 3-benzylidene-d,l-camphor; 2-phenyl-5-methylbenzoxazole; 2,2'-hydroxy-5-methylphenylbenzotriazole; 2-(2'-hydroxy-5'-t-octylphenyl)benzotriazole; 2-(2'-hydroxy-5'-methylphenylbenzotriazole; dibenzalazine; dianisoylmethane; 5-(3,3-dimethyl-2-norbornylidene)-3-pentan-2-one; dibenzoylmethane derivatives such as 4-t-butylmethoxydibenzoylmethane; octyltriazone; urocanic acid derivatives such as urocanic acid and ethyl urocanate; 2-(2'-hydroxy-5'-methylphenyl)benzotriazole, 1-(3,4-dimethoxyphenyl)-4,4-dimethyl-1,3-pentanedione, hydantoin derivatives such as 2-ethylhexyl dimethoxybenzylidene dioxoimidazolidine propionate, phenylbenzimidazolesulfonic acid, terephthalylidene dicamphorsulfonic acid, droxmetrizole trisiloxane, methyl anthranilate, rutin and its derivatives, oryzanol and its derivatives are preferably mentioned.;

[0049] Antibacterial and antiseptic agents include parabens (hydroxybenzoic acid esters) such as methylparaben, ethylparaben, propylparaben, and butylparaben; phenoxyethanol; 1,2-alkanediols such as 1,2-pentanediol, 1,2-hexanediol, and 1,2-octanediol; alkyl glyceryl ethers such as 2-ethylhexylglyceryl ether (ethylhexylglycerin); salicylic acid; lanolin fatty acids and their salts; sodium benzoate; isothiazolinone derivatives such as methylchloroisothiazolinone and methylisothiazolinone; imidazolinium urea; dehydroacetic acid and its salts; phenols; halogenated bisphenols such as triclosan, acid amides, and quaternary ammonium salts; trichlorocarbanide, zinc pyrithione, and benzalkonium chloride. Preferred examples of the antibacterial agent include methyl methyl ether, benzethonium chloride, sorbic acid, chlorhexidine, chlorhexidine gluconate, halocarban, hexachlorophene, and hinokitiol; other phenols such as phenol, isopropylphenol, cresol, thymol, parachlorophenol, phenylphenol, and sodium phenylphenol; phenylethyl alcohol, photosensitizers, antibacterial zeolites, and silver ions. However, when used as an antibacterial agent or preservative for the purpose of preservation, from the viewpoint of safety of cosmetics or topical skin preparations, it is more preferred to use phenoxyethanol; 1,2-alkanediols such as 1,2-pentanediol, 1,2-hexanediol, and 1,2-octanediol; and alkyl glyceryl ethers such as 2-ethylhexyl glyceryl ether.

[0050] Chelating agents include preferably edetates such as EDTA, EDTA2Na, EDTA3Na, EDTA4Na (ethylenediaminetetraacetate); hydroxyethylethylenediaminetriacetates such as HEDTA3Na; pentetates (diethylenetriaminepentaacetate); phytic acid; phosphonic acids such as etidronic acid and salts thereof such as sodium salts; sodium oxalate; polyamino acids such as polyaspartic acid and polyglutamic acid; sodium polyphosphate, sodium metaphosphate, phosphoric acid; sodium citrate, citric acid, alanine, dihydroxyethylglycine, gluconic acid, ascorbic acid, succinic acid, tartaric acid.

[0051] pH adjusters, acids, and alkalis include preferably citric acid, sodium citrate, lactic acid, sodium lactate, glycolic acid, succinic acid, acetic acid, sodium acetate, malic acid, tartaric acid, fumaric acid, phosphoric acid, hydrochloric acid, sulfuric acid, monoethanolamine, diethanolamine, triethanolamine, isopropanolamine, triisopropanolamine, 2-amino-2-methyl-1,3-propanediol, 2-amino-2-hydroxymethyl-1,3-propanediol, arginine, sodium hydroxide, potassium hydroxide, aqueous ammonia, guanidine carbonate, ammonium carbonate.

[0052] Solvents and propellants include lower alcohols such as ethanol, 2-propanol (isopropyl alcohol), butanol, and isobutyl alcohol; glycols such as propylene glycol, 1,3-butylene glycol, diethylene glycol, dipropylene glycol, and isopentyl diol; diethylene glycol monoethyl ether (ethoxydiglycol), ethylene glycol monoethyl ether, ethylene glycol monobutyl ether, triethylene glycol monoethyl ether, diethylene glycol diethyl ether, diethylene glycol dibutyl ether, propylene glycol monoethyl ether, and diethylene glycol diethyl ether. Preferred examples of the propellant include glycol ethers such as propylene glycol monoethyl ether; glycol ether esters such as ethylene glycol monoethyl ether acetate, diethylene glycol monoethyl ether acetate, and propylene glycol monoethyl ether acetate; glycol esters such as diethoxyethyl succinate and ethylene glycol disuccinate; benzyl alcohol, benzyloxyethanol, propylene carbonate, dialkyl carbonate, acetone, ethyl acetate, N-methylpyrrolidone; toluene; fluorocarbons, next-generation chlorofluorocarbons; LPG, dimethyl ether, and carbon dioxide gas.

[0053] Examples of antipruritics include diphenhydramine hydrochloride, chlorpheniramine maleate, camphor, substance-P inhibitors, and the like. Examples of keratolytic and resolvent agents include salicylic acid, sulfur, resorcinol, selenium sulfide, pyridoxine, glycolic acid, and the like. Examples of antiperspirants include hydroxyaluminum chloride, aluminum chloride, zinc oxide, zinc paraphenolsulfonate, and the like. Examples of cooling agents include menthol, methyl salicylate, and the like. Examples of astringents include citric acid, tartaric acid, lactic acid, aluminum potassium sulfate, tannic acid, caffeine, and the like. Examples of enzymes include superoxide dismutase, catalase, lysozyme chloride, lipase, papain, pancreatin, protease, and the like. Preferred examples of nucleic acids include ribonucleic acid and its salts, deoxyribonucleic acid and its salts, and disodium adenosine triphosphate.

[0054] As anti-inflammatory agents, glycyrrhizic acid and its derivatives, glycyrrhetinic acid derivatives, salicylic acid and its derivatives, azelaic acid and its derivatives, hinokitiol, guaiazulene, allantoin, indomethacin, zinc oxide, hydrocortisone acetate, prednisone, diphenhydramine hydrochloride, chlorpheniramine maleate; plant extracts such as peach leaf extract and mugwort leaf extract are preferably mentioned. As hair growth agents, blood circulation promoters, and stimulants, plant extracts and tinctures such as centella extract, capsicum tincture, ginger tincture, ginger extract, cantharis tincture; capsaicin, nonivamide, zingerone, ichthyol, tannic acid, borneol, cyclandelate, cinnarizine, tolazoline, acetylcholine, verapamil, celeferon, γ-oryzanol, celeferon, derivatives such as vitamin E and tocopherol nicotinate · tocopherol acetate, γ-oryzanol, nicotinic acid and derivatives such as nicotinamide · benzyl nicotinate · inositol hexanicotinate, nicotinyl alcohol, allantoin, photosensitizer 301, photosensitizer 401, capronium chloride, monoglyceride pentadecanoate, flavanonol derivative, stigmasterol or stigmastanol and its glycosides, minoxidil are preferably mentioned. As hormones, estradiol, estrone, ethinyl estradiol, cortisone, hydrocortisone, prednisone, etc. are preferably mentioned.

[0055] Anti-wrinkle and anti-aging agents include ascorbic acid, ascorbic acid phosphate salts such as sodium ascorbic acid phosphate and magnesium ascorbic acid phosphate; ascorbic acid fatty acid esters such as ascorbic acid monostearate, ascorbic acid monopalmitate, ascorbic acid dipalmitate, and ascorbic acid tetraisopalmitate; ascorbic acid ethers such as 3-O-ethyl ascorbic acid, 2-O-ethyl ascorbic acid, cetyl ascorbic acid, glyceryl ascorbic acid, and hexylglyceryl ascorbic acid; ascorbic acid glucoside and its fatty acid esters such as ascorbic acid 2-glucoside; and ascorbic acid derivatives such as ascorbic acid sulfate and tocopheryl ascorbyl phosphate. Preferred examples include vitamin A compounds such as retinol, retinol acetate, retinol palmitate, and hydrogenated retinol; nicotinamide, glutathione, cysteine, crocetin, sericin, geraniol, glycerin glucoside, lactoferrin, proanthocyanin, pantothenic acid, panthenol, soybean saponin, revelastol, isoflavone, coenzyme Q10, chondroitin sulfate, acetylglucosamine, glycerophosphatidylcholine, hydrolyzed hyaluronic acid, collagen peptide, conchiolin hydrolysate, adenosine 5'-monophosphate, phosphatidylinositol, trifluoroisopropyloxopropylaminocarbonylpyrrolidinecarbonylmethylpropylaminocarbonylbenzoylaminoacetic acid Na, and tranexamic acid and derivatives thereof.

[0056] Preferred examples of the soothing agent include mannosylerythritol lipid, diethylene glycol oligomer diester of dimer linoleic acid, diisostearyl malate, N-acyl-L-glutamic acid, trehalose monofatty acid ester, fatty acid amide amine oxide, alkyldimethylamine oxide, phenylethyl glucoside, lauryl glucoside, ferulic acid glucoside, salicin, cedrol, polyethylene glycol, polypropylene glycol, and the like. Preferred examples of the cooling agent include menthol, camphor, menthyl lactate, monomethyl succinate, menthyl acetate, borneol, cineole, thymol, peppermint oil, and derivatives thereof. Preferred examples of the warming agent include vanillin and its derivatives, vanillyl amide of nonanoic acid, gingerol, zingerone, capsicum tincture, capsicum extract, benzyl nicotinate, methyl nicotinate, phenyl nicotinate, tocopherol nicotinate and other nicotinic acid derivatives, capsaicin, Dutch pepper extract, sansho extract, ginger extract, cantharides extract, and the like.

[0057] Examples of powders include mica, talc, kaolin, sericite, montmorillonite, kaolinite, mica, white mica, phlogopite, synthetic mica, red mica, biotite, vermiculite, magnesium carbonate, calcium carbonate, aluminum silicate, barium silicate, calcium silicate, magnesium silicate, strontium silicate, metal tungstate salts, magnesium, zeolite, barium sulfate, calcined calcium sulfate, calcium phosphate, fluorapatite, hydroxyapatite, ceramic powder, bentonite, smectite, clay, mud, metal soaps (e.g., zinc myristate, calcium palmitate, aluminum stearate), calcium carbonate, red iron oxide, yellow iron oxide, black iron oxide, ultramarine, cobalt blue, carbon black, titanium oxide, fine and ultrafine titanium oxide, zinc oxide, fine and ultrafine zinc oxide, alumina, silica, fumed silica (ultrafine anhydrous silicic acid), mica titanium, fish scale foil, boron nitride, photochromic pigments, synthetic fluorophlogopite, fine particle composite powders, gold, aluminum, and other inorganic powders of various sizes and shapes, and inorganic powders such as those obtained by treating these with various surface treatment agents such as hydrogen silicone, cyclic hydrogen silicone, or other silanes or titanium coupling agents to make them hydrophobic or hydrophilic; starch, cellulose, nylon powder, polyethylene powder, polymethyl methacrylate powder, polystyrene powder, copolymer resin powder of styrene and acrylic acid, polyester powder, benzoguanamine resin powder, laminated powder of polyethylene terephthalate and polymethyl methacrylate, laminated powder of polyethylene terephthalate, aluminum, and epoxy, etc., urethane powder, silicone powder, Teflon (registered trademark) powder, and other organic powders, surface-treated powders, and organic-inorganic composite powders of various sizes and shapes are preferably used.Examples of inorganic salts include sodium chloride-containing salts such as table salt, refined salt, rock salt, sea salt, and natural salt; potassium chloride, aluminum chloride, calcium chloride, magnesium chloride, bittern, zinc chloride, ammonium chloride; sodium sulfate, aluminum sulfate, aluminum potassium sulfate (alum), aluminum ammonium sulfate, barium sulfate, calcium sulfate, potassium sulfate, magnesium sulfate, zinc sulfate, iron sulfate, copper sulfate; sodium phosphates such as monosodium phosphate, disodium phosphate, trisodium phosphate, potassium phosphates, calcium phosphates, and magnesium phosphates are preferably mentioned.

[0058] Colorants, dyes, and pigments include Brown No. 201, Black No. 401, Purple No. 201, Purple No. 401, Blue No. 1, Blue No. 2, Blue 201, Blue No. 202, Blue No. 203, Blue No. 204, Blue No. 205, Blue No. 403, Blue No. 404, Green No. 3, Green 201, Green 202, Green 204, Green No. 205, Green No. 3, Green 401, and Green 402 No., Red No. 102, Red No. 104-1, Red No. 105-1, Red No. 106, Red No. 2, Red No. 3, Red No. 201, Red No. 202, Red No. 203, Red No. 204, Red No. 205, Red No. 206, Red No. 207, Red No. 208, Red No. 213, Red No. 214, Red No. 215, Red No. 218, Red No. 219, Red No. 220, Red No. 221, Red No. 223 , Red No. 225, Red No. 226, Red No. 227, Red No. 228, Red No. 230-1, Red No. 230-2, Red No. 231, Red No. 232, Red No. 3, Red No. 401, Red No. 40 No. 4, Red No. 405, Red No. 501, Red No. 502, Red No. 503, Red No. 504, Red No. 505, Red No. 506, Orange No. 201, Orange No. 203, Orange No. 204, Orange 20 Legal dyes such as No. 5, Orange 206, Orange 207, Orange 401, Orange 402, Orange 403, Yellow 201, Yellow 202-1, Yellow 202-2, Yellow 203, Yellow 204, Yellow 205, Yellow 4, Yellow 401, Yellow 402, Yellow 403-1, Yellow 404, Yellow 405, Yellow 406, Yellow 407, and Yellow 5; Acid Other acid dyes such as Red 14; basic dyes such as Arianor Sienna Brown, Arianor Madder Red, Arianor Steel Blue, and Arianor Straw Yellow; nitro dyes such as HC Yellow 2, HC Yellow 5, HC Red 3, 4-hydroxypropylamino-3-nitrophenol, N,N'-bis(2-hydroxyethyl)-2-nitro-p-phenylenediamine, HC Blue 2, and Basic Blue 26; disperse dyes; inorganic white pigments such as titanium dioxide and zinc oxide; inorganic red pigments such as iron oxide (red iron oxide) and iron titanate; inorganic brown pigments such as gamma-iron oxide; inorganic yellow pigments such as yellow iron oxide and ochre; inorganic black pigments such as black iron oxide and low-order titanium oxide;Inorganic purple pigments such as mango violet and cobalt violet; inorganic green pigments such as chromium oxide, chromium hydroxide, and cobalt titanate; inorganic blue pigments such as ultramarine and navy blue; pearl pigments such as titanium oxide-coated mica, titanium oxide-coated bismuth oxychloride, titanium oxide-coated talc, colored titanium oxide-coated mica, bismuth oxychloride, and fish scale foil; metal powder pigments such as aluminum powder, copper powder, and gold; anthraquinones such as astaxanthin and alizarin, anthocyanidins, β-carotene, catenares, capsanthin, chalcones, carthamin, quercetin, crocin, chlorophyll, curcumin, cochineal, shikonin, etc., naphthoquinones, bixin, flavones, betacyanidins, henna, hemoglobin, lycopene, riboflavin, rutin, etc., natural pigments and dyes; oxidation dye intermediates and couplers such as p-phenylenediamine, toluene-2,5-diamine, o-, m-, or p-aminophenol, m-phenylenediamine, 5-amino-2-methylphenol, resorcinol, 1-naphthol, 2,6-diaminopyridine, etc. and their salts; auto-oxidation type dyes such as indoline; dihydroxyacetone is preferably mentioned.;

[0059] As spices, there are acetyl cedrene, amyl cinnamaldehyde, allyl amyl glycolate, β-ionone, isoeugenol, isobutyl quinoline, iris oil, iron, indole, ylang-ylang oil, undecanal, undecenal, γ-undecalactone, estragole, eugenol, oakmoss, opoponax resinoid, orange oil, eugenol, aurantiol, galaxolide, carvacrol, L-carboxylic, camphor, canon, carrot seed oil, clove oil, methyl caffeate, geraniol, geranyl nitrile, isobornyl acetate, geranyl acetate, dimethyl benzyl carbinyl acetate, styrallyl acetate, cedryl acetate, terpinyl acetate, p-t-butyl cyclohexyl acetate, vetiveryl acetate, benzyl acetate, linalyl acetate, isopentyl salicylate, benzyl salicylate, sandalwood oil, santalol, cyclamen aldehyde, cyclopentadecanolide, methyl dihydrojasmonate, dihydromyrcenol, jasmine absolute, jasmine lactone, cis-jasmone, citral, citronellol, citronellal, cinnamon bark oil, 1,8-cineole, cinnamaldehyde, styrax resinoid, cedarwood oil, cedrene, cedrol, celery seed oil, thyme oil, damascone, damasconen, thymol, tuberose absolute, decanal, decalactone, terpineol, γ-terpinene, triplal, nerol, nonanal, 2,6-Nonadienol, nonalactone, patchouli alcohol, vanilla absolute, vanillin, basil oil, patchouli oil, hydroxycitronellal, α-pinene, piperitone, phenethyl alcohol, phenylacetaldehyde, ptychopetalum oil, hexyl cinnamaldehyde, cis-3-hexenol, Peru balsam, vetiver oil, vetiverol, peppermint oil, pepper oil, heliotropin, bergamot oil, benzyl benzoate, borneol, myrrh resinoid, musk ketone, methyl nonyl acetaldehyde, γ-methyl ionone, menthol, L-menthol, L-menthone, eucalyptus oil, β-ionone, lime oil, lavender oil, D-limonene, linalool, lilial, lilyal, lemon oil, rose absolute, rose oxide, rose oil, rosemary oil, synthetic fragrances and natural fragrances such as various essential oils, and various compounded fragrances are preferably mentioned.

[0060] As water, in addition to ordinary water and purified water, hard water, soft water, natural water, deep ocean water, electrolyzed alkaline ion water, electrolyzed acidic ion water, ion water, and cluster water are preferably mentioned.

[0061] In addition to these, components described in Cosmetic Raw Material Standards, Cosmetic Type Blending Component Standards, the List of Component Display Names of the Japan Cosmetic Industry Association, the INCI Dictionary (The International Cosmetic Ingredient Dictionary and Handbook), Quasi-Drug Raw Material Standards, the Japanese Pharmacopoeia, Pharmaceutical Additive Standards, the Official Monographs of Food Additives, etc., and components described in Japanese and foreign patent gazettes and patent published gazettes (including published gazettes and re-published gazettes) belonging to the classifications of A61K7 and A61K8 in the International Patent Classification IPC, etc., known cosmetic components, pharmaceutical components, food components, etc. can be contained in known combinations and blending ratios and amounts.

[0062] Examples of the types of cosmetics of the present invention include hair cosmetics, skin cosmetics, makeup cosmetics, fragrance cosmetics, body cosmetics, etc. The cosmetics of the present invention can be produced according to ordinary methods.

[0063] To explain the types of cosmetics of the present invention in more detail, preferred examples of hair cosmetics include shampoos such as oil shampoo, cream shampoo, conditioning shampoo, anti-dandruff shampoo, hair color shampoo, and rinse-in-one shampoo; hair care agents such as rinse, conditioner, treatment, hair pack, leave-on treatment, hair mist, and hair oil; hair styling products such as hair foam, hair mousse, hair spray, hair wax, hair gel, hair cream, water grease, setting lotion, pomade, and tic; hair dyes such as color lotions, hair color treatments, hair manicures, and oxidation hair dyes; hair tonics, hair liquids, hair blow dryers, split end coats, permanent wave agents, straight perm agents, hair bleach, hair color pre-treatments, hair color after-treatments, perm pre-treatments, perm after-treatments, and hair growth agents.

[0064] Examples of skin cosmetics include lotions such as softening lotion, astringent lotion, cleansing lotion, multi-layered lotion, liposome lotion, etc.; emulsions such as emollient emulsion, moisturizing emulsion, milky lotion, nourishing lotion, nourishing milk, skin moisturizer, moisturizing emulsion, massage lotion, cutin smoother, elbow lotion, hand lotion, body lotion, etc.; creams such as emollient cream, nourishing cream, nourishing cream, vanishing cream, moisturizing cream, night cream, massage cream, cleansing cream, makeup cream, base cream, pre-makeup cream, sunscreen cream, suntan cream, hair removal cream, deodorant cream, shaving cream, cutin softening cream, etc.; gels such as moisturizing gel, whitening gel, all-in-one gel, etc.; beauty essences such as moisturizing essence, whitening essence, moisturizing serum, whitening serum, etc. Skin care cosmetics; sunscreen cosmetics such as sun protect, sun protector, UV care milk, sunscreen, etc.; pack and mask products such as peel-off pack, powder pack, washing pack, oil pack, cleansing mask, etc.; cleansing cosmetics such as cleansing foam, cleansing cream, cleansing milk, cleansing lotion, cleansing gel, cleansing oil, etc.; facial cleansing products such as paste facial cleansing foam, gel facial cleansing foam, foaming facial cleansing foam, facial cleansing powder, cosmetic soap, transparent soap, medicinal soap, liquid soap, beard shaving soap, etc. are preferably mentioned.

[0065] Examples of makeup cosmetics preferably include lipstick, lip gloss, foundation, blush, face powder, concealer, eyeliner, mascara, eyeshadow, eyebrow pencil, eyebrow brush, nail enamel, enamel remover, nail treatment.

[0066] Examples of fragrance cosmetics preferably include perfume, perfume, parfum, eau de parfum, eau de toilette, eau de cologne, compound perfume, fragrant powder, perfume soap, body lotion, bath oil.

[0067] Preferred examples of body cosmetics include body cleansers such as body shampoos, deodorant cosmetics such as deodorant lotions, deodorant powders, deodorant sprays and deodorant sticks, bleaches, depilatories, bath additives, and insect repellents such as insect repellent sprays.

[0068] Preferred types of the external skin preparation of the present invention include ointments, lotions, creams or gels for external skin applications, patches, liniments, liquid applications, etc. They can also be used as oral cosmetics such as toothpastes and mouthwashes.

[0069] Preferred dosage forms of the cosmetic or topical skin preparation of the present invention include emulsion-type cosmetics such as oil-in-water (O / W) type, water-in-oil (W / O) type, W / O / W type, and O / W / O type, oil-based cosmetics, solid cosmetics, liquid cosmetics, paste cosmetics, stick-type cosmetics, volatile oil-type cosmetics, powder cosmetics, jelly-type cosmetics, gel-type cosmetics, paste-type cosmetics, emulsified polymer-type cosmetics, sheet-type cosmetics, mist-type cosmetics, and spray-type cosmetics.

[0070] The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples. In these examples, the zeta potential was measured using a Zetasizer Nano-ZS manufactured by Malvern Instruments.

[0071] <Preparation of the Complex of the Present Invention 1> The complexes of the present invention were prepared using the compositions shown in Tables 1 and 2 by the method described below. Using the resulting complexes, liposome solutions were prepared according to the liposome solution formulations described below. The zeta potential and pH of the resulting liposome solutions were measured, and the dispersion stability was evaluated. The results are shown in the lower part of Tables 1 and 2. Method for producing a complex Each component of the complex of the present invention (10 g in total) was dissolved in 80 g of cyclohexane, then instantly frozen in liquid nitrogen and freeze-dried in a freeze-dryer (Tokyo Rikakikai, FDU-2200 model). The pressure was 10 Pa or less and the trap temperature was -80°C. All of the complexes obtained in this manner were homogeneous powders. Liposome solution formulation Component Amount (mass%) --------------------------------- Complex 0.2 1,3-butylene glycol 10.0 Methylparaben 0.1 Purified water 89.7 --------------------------------- Method for preparing a liposome solution The complex was added to 1,3-butylene glycol and stirred and mixed at 80°C to prepare a polyhydric alcohol solution. The resulting polyhydric alcohol solution was added to purified water in which methylparaben had been dissolved while stirring at 80°C, and the mixture was stirred with a homomixer (75°C, 5000 rpm, 15 minutes) and cooled. Method for evaluating dispersion stability The obtained liposome solution was stored at 40°C for 2 weeks, and then the appearance was visually observed, and the dispersion stability was evaluated according to the following evaluation criteria. ◎ No change in appearance from the initial The appearance has changed slightly since the early days. △... The appearance has changed a little since the early days. ×... The appearance has changed significantly since the early days

[0072] [Table 1]

[0073] [Table 2]

[0074] From the results in Tables 1 and 2, it was found that the complex of the present invention containing component (A) and component (B) as liposome membrane components can obtain liposomes with a positive zeta potential, that is, with a positively charged surface, by dispersing them in water. The zeta potential of the complex without component (B) was negative. Also, from the results in Table 1, when lecithin with a PC content of 96% by mass was used as component (A), it was found that the zeta potential becomes positive when component (B) is blended in an amount of 0.2 parts by mass or more based on 100 parts by mass of component (A). Further, from the results in Table 2, when lecithin with a PC content of 76% by mass was used as component (A), it was considered that the zeta potential becomes positive when component (B) is blended in an amount more than 2.0 parts by mass based on 100 parts by mass of component (A).

[0075] <Preparation 2 of the complex of the present invention> With the composition in Table 3, a complex was prepared by blending phytosterol of component (C) as a liposome membrane component in the same manner as described above. Using the obtained complex, a liposome solution was prepared in the same manner as described above. For the obtained liposome solution, the zeta potential and pH were measured, and the long-term dispersion stability was evaluated. The results are also shown in the lower part of Table 3. Method for evaluating long-term dispersion stability For the obtained liposome solution, the appearance after storage at 40 °C for 6 months was visually observed, and the dispersion stability was evaluated according to the following evaluation criteria. ◎··· No change in appearance from the initial state 〇··· Slight change in appearance from the initial state △··· Some change in appearance from the initial state ×··· Great change in appearance from the initial state

[0076]

Table 3

[0077] The results in Table 3 show that liposomes prepared from the complex of the present invention have excellent long-term dispersion stability when further blended with component (C) as a liposome membrane component.

[0078] <Preparation of the Complex of the Present Invention 3> A complex of the present invention was prepared using the composition shown in Table 4 in the same manner as described above. 0.2 parts by mass of the resulting complex was added to 99.8 parts by mass of purified water while stirring at 80°C, and the mixture was stirred in a homomixer (75°C, 5000 rpm, 15 minutes) and cooled. The zeta potential of the resulting liposome solution was evaluated. The results are shown in the lower part of Table 4.

[0079] [Table 4]

[0080] The results in Table 4 demonstrate that the complex of the present invention can be easily dispersed in water to give liposomes with a positively charged surface, and that various oil-soluble active ingredients can be contained therein.

[0081] <Evaluation of storage stability> The composites of the present invention were prepared using the compositions shown in Table 5 in the same manner as described above. The storage stability of the obtained composites was evaluated using the method described below. The results are also shown in the lower part of Table 5. Method for evaluating storage stability The complex of the present invention was stored at 50°C for 1 month or 3 months. 0.2 parts by mass of the complex after storage was added to 99.8 parts by mass of purified water at 80°C while stirring, and the mixture was stirred in a homomixer (75°C, 5000 rpm, 15 minutes) and cooled. The appearance of the resulting liposome solution was visually observed, and the storage stability was evaluated according to the following evaluation criteria. ◎ No insoluble matter in the liposome solution ○: There is a small amount of insoluble matter in the liposome solution. △: A small amount of insoluble matter in the liposome solution ×: There is a large amount of insoluble matter in the liposome solution.

[0082] [Table 5]

[0083] The results in Table 5 show that when the complex of Example 11, which does not contain lactic acid, was dispersed in water after long-term storage, the presence of insoluble matter was confirmed in the liposome solution. Since such a phenomenon was not observed in Comparative Example 5, which does not contain phytosphingosine, it is presumed that this is because highly crystalline phytosphingosine precipitated from the complex during long-term storage. On the other hand, it was confirmed that such a phenomenon was suppressed in the complex containing lactic acid. Therefore, it was found that the storage stability of the complex of the present invention was improved (crystal precipitation over time was suppressed) by incorporating lactic acid, an organic acid.

[0084] <Evaluation of usability> A liposome solution was prepared using the complex of Example 3 of the present invention or the complex of Comparative Example 1 not containing component (B) of the present invention, with the composition shown in Table 6. The resulting liposome solution was applied to the skin by the following method, and the sensation upon use was evaluated. The results are shown in Table 6. Method for evaluating usability The results were expressed as the average score of the five panelists' ratings based on the following criteria: Evaluation criteria 5 points: Much better than the liposome solution of Comparative Example 1 4 points: Better than the liposome solution of Comparative Example 1 3 points: Equivalent to the liposome solution of Comparative Example 1 2 points: worse than the liposome solution of Comparative Example 1 1 point: Significantly worse than the liposome solution of Comparative Example 1 Method for preparing a liposome solution The complex was added to 1,3-butylene glycol and stirred and mixed at 80°C to prepare a polyhydric alcohol solution. The obtained polyhydric alcohol solution was added to purified water in which methylparaben had been previously dissolved, while stirring at 80°C, and stirred with a homomixer (75°C, 5000 rpm, 15 minutes), and then cooled.

[0085]

Table 6

[0086] From the results in Table 6, it was found that the liposomes prepared using the complex of the present invention were superior in terms of usability such as ease of spreading during application, lack of stickiness during drying, and moist feeling after drying, compared to the liposomes prepared using the complex not containing component (B) of the present invention.

[0087] <Evaluation 1 of Skin Penetration> Using Nile red, an oil-soluble fluorescent dye, as an index for the skin penetration of the oil-soluble active ingredient, the penetration of the active ingredient of the liposome solution prepared using the complex of the present invention into the skin was evaluated by the method described below. The results are shown in the lower part of Table 7. Skin penetration test method Using the composition described in Table 7, a liposome solution encapsulating Nile Red in the liposome membrane was prepared using the complex of the present invention. As a control, a liposome solution was similarly prepared using a complex that does not contain component (B) of the present invention. Next, a 3D skin model (LabCyte EPI-MODEL) manufactured by J-TEC was sandwiched between silicone plates with holes having a diameter of 5 mm, and the receptor cells were placed in a Franz-type diffusion cell filled with PBS(-). 200 μL of the liposome solution was applied to the skin surface, and after standing for 6 hours in an environment at a temperature of 37 °C and a relative humidity of 80%, the skin model was washed with PBS(-) to remove the liposomes adhering to the surface, and then dissolved in 100 mM Tris-HCl buffer (pH 8.0) (containing 1% Triton X-100). The lysate was centrifuged, and the fluorescence intensity of the supernatant after centrifugation was measured using a microplate reader (excitation wavelength 553 nm, fluorescence wavelength 637 nm). In addition, the protein concentration in the supernatant was measured by the BCA method. The fluorescence intensity value per 1 μg of protein was calculated from the measured fluorescence intensity and protein concentration, and this fluorescence intensity value was used as an index of skin permeability. The results were shown as relative values when the fluorescence intensity value of the control was set to 100. Method for preparing a liposome solution The complex and Nile Red were added to 1,3-butylene glycol, and stirred and mixed at 80 °C to prepare a polyhydric alcohol solution. The obtained polyhydric alcohol solution was added to purified water in which methylparaben had been previously dissolved, while stirring at 80 °C, and stirred with a homomixer (75 °C, 5000 rpm, 15 minutes), and then cooled.

[0088] [Table 7]

[0089] From the results in Table 7, it was considered that the liposomes prepared using the complex of the present invention were superior in the skin permeability of the oil-soluble active ingredient compared to the liposomes prepared using the complex that does not contain component (B) of the present invention.

[0090] <Skin penetration evaluation 2> Using fluoresceinamine-labeled sodium hyaluronate as an index of skin permeability of water-soluble active ingredients, the skin permeability of the active ingredients in the liposome solution prepared using the complex of the present invention was evaluated by the method described below.The results are shown in the bottom row of Table 8. Skin penetration test method A liposome solution of the present invention was prepared using the complex of the present invention with the composition shown in Table 8, and fluoresceinamine-labeled sodium hyaluronate (FAHA-L2, manufactured by PG Research; molecular weight: 100,000 to 300,000) added. As a control, a liposome solution was prepared in the same manner using a complex without component (B) of the present invention. Next, a three-dimensional skin model (LabCyte EPI-MODEL, manufactured by J-TEC) was sandwiched between silicone plates with 5 mm diameter holes and placed in a Franz-type diffusion cell with the receptor cell filled with PBS(-). 200 μL of liposome solution was applied to the skin surface and allowed to stand for 6 hours at 37°C and 80% relative humidity. The skin model was then washed with PBS(-) to remove liposomes adhering to the surface. The resulting skin model was embedded in OCT compound (manufactured by Sakura Finetech) and rapidly frozen, after which cross-sectional sections of the skin were prepared using a cryostat microtome (manufactured by Sakura Finetech). The obtained skin sections were observed under a fluorescence microscope (Keyence BZ-X800) to measure the fluorescence intensity (excitation wavelength 470 nm, fluorescence wavelength 525 nm) and used as an index of the permeability of sodium hyaluronate into the skin. The results are shown as relative values, with the fluorescence intensity of the control set at 100. Method for preparing a liposome solution The complex was added to 1,3-butylene glycol and stirred at 80°C to prepare a polyhydric alcohol solution. The resulting polyhydric alcohol solution was added to purified water in which methylparaben had been dissolved while stirring at 80°C, and the mixture was stirred with a homomixer (75°C, 5000 rpm, 15 minutes) and cooled. Then, fluoresceinamine-labeled hyaluronic acid Na was added and stirred.

[0091]

Table 8

[0092] From the results in Table 8, it was considered that the liposomes prepared using the complex of the present invention were superior in the permeability of the water-soluble active ingredient to the skin compared to the liposomes prepared using the complex not containing component (B) of the present invention.

[0093] The following cosmetics were prepared. These cosmetics are excellent in the permeability of the active ingredient, have excellent usability, are also excellent in moisture retention, and contribute to improving the firmness of the skin.

[0094] Example 22 Liposome Solution Component Blending amount (wt%) -------------------------------------- Part A Complex of Example 18 0.3 1,3-Butylene glycol 9.0 Part B Phenoxyethanol 0.5 Purified water An amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0095] Example 23 Liposome Solution Component Blending amount (wt%) -------------------------------------- Part A Complex of Example 19 0.5 Dipropylene glycol 10.0 Part B Phenoxyethanol 0.3 Purified water An amount to make a total of 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated to about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0096] Example 24 Liposome Solution Component Dosage (wt%) -------------------------------------- Part A Complex of Example 19 0.5 Ethanol 8.0 1,3 - Butylene Glycol 5.0 Part B Phenoxyethanol 0.3 Purified Water An amount that totals 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated to about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0097] Example 25 Liposome Solution Component Dosage (wt%) -------------------------------------- Part A Complex of Example 19 0.2 Ethanol 8.0 Dipropylene Glycol 5.0 Part B Phenoxyethanol 0.3 Purified Water An amount that totals 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated to approximately 70°C. While stirring with a propeller (600 rpm), Part A was added to Part B and then cooled.

[0098] Example 26 Liposome Solution Component Dosage (wt%) -------------------------------------- Part A The complex of Example 19 0.5 Part B Phenoxyethanol 0.3 Purified water An amount such that the total is 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated to approximately 70°C. Part A was added to Part B, gently mixed, then passed through a high-pressure emulsifier twice (70 MPa), and the resulting dispersion was cooled.

[0099] Example 27 Liposome Solution Component Dosage (wt%) -------------------------------------- Part A The complex of Example 18 0.5 1,3-Butylene glycol 10.0 Ceramide 3 0.05 Ceramide 6 0.02 Part B Phenoxyethanol 0.5 Purified water An amount such that the total is 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated to approximately 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0100] Example 28 Liposome Solution Component Blending Ratio (wt%) -------------------------------------- Part A Composite of Example 18 0.5 Propanediol 10.0 Ceramide 3 0.04 Ceramide 6 0.03 Part B Phenoxyethanol 0.3 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated at about 70°C. Part A was added to Part B and gently mixed, then passed through a high-pressure emulsifier twice (100 MPa), and the resulting dispersion was cooled.

[0101] Example 29 Liposome Solution Component Blending Ratio (wt%) -------------------------------------- Part A Composite of Example 18 0.2 1,3-Butylene Glycol 10.0 Ceramide 3 0.01 Part B Phenoxyethanol 0.3 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated at about 80°C. Part A was added to Part B while stirring with a homomixer (5000 rpm), and then cooled.

[0102] Example 30 Liposome Solution Component Blending Ratio (wt%) -------------------------------------- Part A Composite of Example 18 0.2 1,3-Butylene glycol 15.0 Lysophosphatidylcholine (lysophosphatidylcholine content 70% or more) 0.02 Part B Phenoxyethanol 0.5 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 70°C. While stirring with a propeller (600 rpm), Part A was added to Part B and then cooled.

[0103] Example 31 Skin lotion Components Blending amount (wt%) -------------------------------------- Part A Composite of Example 19 0.3 1,3-Butylene glycol 9.0 Hydrogenated lysophosphatidylcholine (lysophosphatidylcholine content 70% or more) 0.1 Tsubokusa extract 0.1 Part B Glyceryl caprylate 0.3 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 70°C. While stirring with a propeller (600 rpm), Part A was added to Part B and then cooled.

[0104] Example 32 Skin lotion Components Blending amount (wt%) -------------------------------------- [[ID=P59]]A part Composite of Example 18 0.6 1,3-Butylene glycol 10.0 Hydrogenated lysophosphatidylcholine (lysophosphatidylcholine content 70% or more) 0.04 Ceramide 3 0.01 Part B Phenoxyethanol 0.5 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0105] Example 33 Skin Lotion Ingredient Dosage (wt%) -------------------------------------- Part A The complex of Example 18 0.6 1,3-Butylene glycol 10.0 Lysophosphatidylcholine (lysophosphatidylcholine content 70% or more) 0.1 Tsubokusa extract 0.01 Cholesterol 0.01 γ-Oryzanol 0.05 Part B Phenoxyethanol 0.5 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0106] Example 34 Skin Lotion Ingredient Dosage (wt%) -------------------------------------- Part A The complex of Example 19 0.4 1,3-butylene glycol 10.0 Stearyl Glycyrrhetinate 0.1 Part B Phenoxyethanol 0.5 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part A and Part B were each heated to approximately 80° C. Part A was added to Part B while stirring with a homomixer (5000 rpm), and the mixture was cooled.

[0107] Example 35 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.3 1,3-butylene glycol 8.0 1,2-Pentanediol 2.0 Tocopherol nicotinate 0.1 Ceramide 3 0.01 Part B Glyceryl Caprylate 0.1 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part A and Part B were each heated to approximately 80° C. Part A was added to Part B while stirring with a homomixer (5000 rpm), and the mixture was cooled.

[0108] Example 36 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 12 0.2 1,3-butylene glycol 10.0 Part B Tranexamic acid (Nippon Seika) 2.0 Citric acid appropriate amount Phenoxyethanol 0.1 Purified water an amount that totals 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at approximately 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled. The pH was adjusted to around 5 with citric acid.

[0109] Example 37 Lotion Ingredient Blending amount (wt%) -------------------------------------- Part A Complex of Example 14 0.2 1,3-Butylene glycol 10.0 Part B Purified water an amount that totals 100 Part C 3-O-Ethylascorbic acid (Nippon Seika) 1.5 Citric acid appropriate amount Sodium citrate 0.2 Phenoxyethanol 0.1 Ethanol 10.0 -------------------------------------- (Preparation method) Part A and Part B were each heated at approximately 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled. Part C was added and the pH was adjusted to around 4.5 with citric acid.

[0110] Example 38 Lotion Ingredient Blending amount (wt%) -------------------------------------- Part A Composite of Example 15 0.2 1,3-Butylene glycol 10.0 Part B Purified water in an amount to make a total of 100 Part C 3-O-Ethyl ascorbic acid (Nippon Fine Chemical) 1.5 Citric acid appropriate amount Sodium citrate 0.2 Phenoxyethanol 0.1 Ethanol 10.0 Hydrophobized hydroxypropyl methylcellulose 0.2 Sodium pentetate 0.1 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and cooled. Part C was added, and the pH was adjusted to around 4.5 with citric acid.

[0111] Lotion of Example 39 Component Blending amount (wt%) -------------------------------------- Part A Composite of Example 19 0.4 1,3-Butylene glycol 10.0 Part B Purified water in an amount to make a total of 100 Part C 3-O-Ethyl ascorbic acid (Nippon Fine Chemical) 1.0 Tranexamic acid (Nippon Fine Chemical) 1.0 Arbutin (Nippon Fine Chemical) 3.2 Citric acid appropriate amount Sodium citrate 0.1 Phenoxyethanol 0.1 Ethanol 10.0 Sodium pyrosulfite 0.1 -------------------------------------- (Preparation method) Part A and Part B were each heated to approximately 80°C. Part A was added to Part B while stirring with a homomixer (5000 rpm) and cooled. Part C was added, and the pH was adjusted to around 4.5 with citric acid.

[0112] Example 40: Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.3 1,3-butylene glycol 10.0 Part B Purified water (amount to make a total of 100) C part Sodium hyaluronate (molecular weight 50,000 to 500,000) 0.1 D part Sodium hyaluronate (molecular weight 1 million to 1.5 million) 0.1 Glycyrrhizic acid 2K 0.2 3-O-Ethyl ascorbic acid (Nippon Fine Chemical) 1.0 Tranexamic acid (Nippon Fine Chemical) 1.0 Arbutin (Nippon Fine Chemical) 6.5 Citric acid (appropriate amount) Sodium citrate 0.2 Phenoxyethanol 0.1 Ethanol 10.0 Sodium pyrosulfite 0.05 -------------------------------------- (Preparation method) Parts A and B were heated to approximately 80°C. Part A was added to part B while stirring with a homomixer (5000 rpm) and cooled. Parts C and D were added, and citric acid was added to adjust the pH to around 5.

[0113] Example 41 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.2 1,3-butylene glycol 10.0 Part B Purified water (amount to make a total of 100) C part Sodium hyaluronate (molecular weight 300,000 to 1,000,000) 0.1 D part Glycyrrhizic acid 2K 0.2 Heparinoid 0.1 Pullulan 0.05 Hydrolyzed Collagen 0.1 Citric acid (appropriate amount) Sodium citrate 0.1 Phenoxyethanol 0.3 Artemisia capillaris extract 0.3 -------------------------------------- (Preparation method) Parts A and B were heated to approximately 80°C. Part A was added to part B while stirring with a homomixer (5000 rpm) and cooled. Parts C and D were added, and the pH was adjusted to around 5.5 with citric acid.

[0114] Example 42 Lotion Component Amount (% by weight) -------------------------------------- Part A Complex of Example 16 0.2 1,3-butylene glycol 10.0 Part B Purified water (amount to make a total of 100) C part Carbomer (2% aqueous solution, neutralized with KOH) 10.0 D part Glycyrrhizic acid 2K 0.1 Tremoist-SL (Nippon Fine Chemical) 5.0 Artemisia capillaris extract 0.3 -------------------------------------- (Preparation method) Parts A and B were heated to approximately 80°C. Part A was added to part B while stirring with a homomixer (5000 rpm) and cooled. Part C was added and homogenized, and then part D was added and stirred until homogenized.

[0115] Example 43 Lotion Component Amount (% by weight) -------------------------------------- Part A Complex of Example 19 0.2 1,3-butylene glycol 2.0 Dipropylene Glycol 3.0 Part B Purified water (amount to make a total of 100) C part Sodium hyaluronate (molecular weight 1.5 million to 2 million) 0.1 D part Carbomer (2% aqueous solution, neutralized with KOH) 10.0 Glyceryl Caprylate 0.3 -------------------------------------- (Preparation method) Parts A and B were heated to approximately 80°C. Part A was added to part B while stirring with a homomixer (5000 rpm) and cooled. Part C was added and homogenized, and then part D was added and stirred until homogenized.

[0116] Example 44 Lotion Component Amount (% by weight) -------------------------------------- Part A Complex of Example 17 0.2 Glycerin 3.0 1,2-Hexanediol 1.0 Part B Phenoxyethanol 0.5 Purified water in an amount such that the total is 100 -------------------------------------- (Preparation method) Part A and Part B were each heated to about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0117] Example 45 Lotion Component Blending amount (wt%) -------------------------------------- Part A Composite of Example 19 0.2 PrimeLipid PI (Nippon Fine Chemical) 0.1 Glycerin 3.0 1,2 - Pentanediol 2.0 Part B Phenoxyethanol 0.5 Purified water in an amount such that the total is 100 -------------------------------------- (Preparation method) Part A and Part B were each heated to about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0118] Example 46 Lotion Component Blending amount (wt%) -------------------------------------- Part A Composite of Example 13 0.4 1,3 - Butylene glycol 5.0 1,2 - Pentanediol 2.0 Propylene glycol 1.0 Part B Phenoxyethanol 0.5 Purified water in an amount such that the total is 100 -------------------------------------- (Preparation Method) Part A and Part B were each heated to approximately 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0119] Example 47 Lotion Component Dosage (wt%) -------------------------------------- Part A Composite of Example 19 0.6 Dipropylene Glycol 8.0 1,2 - Pentanediol 2.0 Ceramide 1 0.03 Ceramide 2 0.03 Ceramide 3 0.1 Ceramide 4 0.02 Ceramide 5 0.01 Ceramide 6 0.05 Part B Phenoxyethanol 0.5 Purified Water An amount to make 100 in total -------------------------------------- (Preparation Method) Part A and Part B were each heated to approximately 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0120] Example 48 Lotion Component Dosage (wt%) -------------------------------------- Part A Composite of Example 19 0.3 1,3 - Butylene Glycol 6.0 Ethanol 2.0 Astaxanthin 0.01 Tocopherol 0.03 Part B Artemisia capillaris extract 0.1 Glucosylrutin 0.1 Ethylhexylglycerin 1.5 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part A and Part B were each heated to approximately 80° C. Part A was added to Part B while stirring with a homomixer (5000 rpm), and the mixture was cooled.

[0121] Example 49 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.4 Propylene glycol 6.0 Dipropylene Glycol 4.0 Ubiquinone 0.1 Part B Artemisia capillaris extract 0.1 Ethylhexylglycerin 1.0 Etidronic acid tetrasodium 0.05 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part A and Part B were each heated to approximately 80° C. Part A was added to Part B while stirring with a homomixer (5000 rpm), and the mixture was cooled.

[0122] Example 50 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.4 1,3-butylene glycol 6.0 Dipropylene glycol 4.0 Part B Purified water in an amount such that the total is 100 Part C Inulin-SC (Nippon Seika) 0.7 Ascorbic acid 2-glucoside 2.0 Potassium hydroxide appropriate amount Disodium edetate 0.1 Phenoxyethanol 0.01 Ethylhexylglycerin 0.8 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and cooled. Part C was added, and the pH was adjusted to around 6 with potassium hydroxide.

[0123] Example 51 Lotion Component Blending amount (wt%) -------------------------------------- Part A Complex of Example 19 0.4 1,3-Butylene glycol 6.0 Dipropylene glycol 4.0 PrimeLipid PI (Nippon Seika) 0.2 Part B Purified water in an amount such that the total is 100 Part C Inulin-SC (Nippon Seika) 1.0 Ascorbic acid 2-glucoside 2.0 Potassium hydroxide appropriate amount Sodium etidronate 0.1 Phenoxyethanol 0.01 Ethylhexylglycerin 0.8 -------------------------------------- (Preparation method) The A part and the B part were each heated to about 80°C. While stirring with a homomixer (5000 rpm), the A part was added to the B part and then cooled. The C part was added, and the pH was adjusted to around 6 with potassium hydroxide.

[0124] Example 52 Lotion Component Dosage (wt%) -------------------------------------- Part A Composite of Example 19 0.1 1,3-Butylene glycol 6.0 Part B Purified water An amount that totals 100 Part C Ascorbic acid 1.0 Ethanol 10.0 Citric acid Appropriate amount Sodium citrate 0.1 EDTA-2Na 0.1 Phenoxyethanol 0.3 Ethylhexylglycerin 0.8 -------------------------------------- (Preparation method) The A part and the B part were each heated to about 80°C. While stirring with a homomixer (5000 rpm), the A part was added to the B part and then cooled. The C part was added, and the pH was adjusted to around 5 with citric acid.

[0125] Example 53 Lotion Component Dosage (wt%) -------------------------------------- Part A Composite of Example 19 0.2 1,3-Butylene glycol 6.0 Part B Purified water An amount that totals 100 Part C Sodium pentetate 0.07 EDTA-4Na 0.03 Phenoxyethanol 0.3 Ethylhexylglycerin 0.8 Neosolue-AquaS (Nippon Fine Chemical) 1.0 -------------------------------------- (Preparation method) Part A and Part B were each heated to about 80° C. Part A was added to Part B while stirring with a homomixer (5000 rpm), cooled, and then Part C was added.

[0126] Example 54 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.2 1,3-butylene glycol 6.0 Part B Purified water (amount to make a total of 100) C part Carbomer (2% aqueous solution, neutralized with KOH) 5.0 EDTA-4Na 0.03 Phenoxyethanol 0.3 Ethylhexylglycerin 0.8 Neosolue-Aqulio (Nippon Fine Chemical) 0.5 -------------------------------------- (Preparation method) Part A and Part B were each heated to about 80° C. Part A was added to Part B while stirring with a homomixer (5000 rpm), cooled, and then Part C was added.

[0127] Example 55 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.2 1,3-butylene glycol 6.0 Part B Purified water (amount to make a total of 100) C part Carbomer (2% aqueous solution, neutralized with KOH) 5.0 Glyceryl Caprylate 0.1 Ethylhexylglycerin 0.3 Neosolue-Aqulio (Nippon Fine Chemical) 1.0 D part PrimeLipid ALPA (Nippon Fine Chemical) 0.1 -------------------------------------- (Preparation method) Parts A and B were each heated to approximately 70°C. Part A was added to part B and mixed gently, then passed through a high-pressure emulsifier twice (100 MPa). The resulting dispersion was cooled, and parts C and D were added, followed by stirring until uniform.

[0128] Example 56 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.5 Dipropylene glycol 8.0 Ethanol 4.0 Plandool-LG1 (Nippon Fine Chemical) 0.05 Part B Purified water (amount to make a total of 100) C part Sodium hyaluronate (molecular weight 50,000 to 500,000) 0.1 D part (Acrylic acid / lauryl methacrylate / isodecyl methacrylate) crosspolymer (3% aqueous solution, NaOH neutralized) 3.0 Glyceryl Caprylate 0.1 Neosolue-AquaS (Nippon Fine Chemical) 1.5 -------------------------------------- (Preparation method) Parts A and B were each heated to about 80° C. Part A was added to part B while stirring with a homomixer (5000 rpm), and the mixture was cooled, followed by addition of parts C and D.

[0129] Example 57 Lotion Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 1.0 Dipropylene glycol 8.0 Ethanol 4.0 1,2-Pentanediol 1.0 Plandool-MAS (Nippon Fine Chemicals) 0.1 Part B Purified water (amount to make a total of 100) C part Hydrophobized hydroxypropyl methylcellulose 0.2 (PEG-240 / Decyltetradeceth-20 / HDI) copolymer 0.02 Glyceryl Caprylate 0.5 Neosolue-Aqulio (Nippon Fine Chemical) 1.0 -------------------------------------- (Preparation method) Parts A and B were each heated to about 80° C. Part A was added to part B while stirring with a homomixer (5000 rpm), and the mixture was cooled, followed by addition of parts C and D.

[0130] Example 58 Emulsion Component Amount (wt%) -------------------------------------- Part A Phytocompo-PP (Nippon Fine Chemical) 1.0 Glycerin 5.0 1,3-butylene glycol 9.0 Part B Plandool-LG1 (refined in Japan) 1.0 Plandool-LG2 (refined in Japan) 1.0 Plandool-LG3 (refined in Japan) 1.0 Plandool-MAS (refined in Japan) 1.0 LUSPLAN PI-DA (refined in Japan) 0.5 Cetyl ethylhexanoate 5.0 Cetanol 1.2 Oleyl alcohol 1.0 Cholesterol 0.8 Part C Carbomer (2% aqueous solution, neutralized with KOH) 5.0 Phenoxyethanol 0.3 Part D Purified water in an amount to make a total of 100 Part E Liposome solution of Example 24 30.0 -------------------------------------- (Preparation method) Part A was heated to 70 °C and uniformly dispersed. After Part B was heated to 70 °C and uniformly dissolved, it was added to Part A and uniformly dispersed (Part F). Part D, which had been preheated to about 70 °C, was added to Part F and emulsified using a homomixer. After cooling to around 40 °C, Part C and Part E were added and stirred with a propeller to mix uniformly.

[0131] Example 59 Emulsion Component Dosage (wt%) -------------------------------------- Part A Phytocompo-C (refined in Japan) 1.0 Glycerin 6.0 1,3-Butylene glycol 8.0 Part B LUSPLAN SR-DM4 (refined in Japan) 1.0 Plandool-MAS (refined in Japan) 0.3 Glyceryl Tri(caprylate / caprate) 3.0 Stearyl Alcohol 2.5 Arachidyl Alcohol 1.0 Isostearyl Glyceryl Ether 0.5 Part C Carbomer (2% aqueous solution, neutralized with KOH) 5.0 Phenoxyethanol 0.3 Part D Purified Water in an amount to make 100 in total Part E Liposome solution of Example 28 30.0 ------------------------------------- (Preparation Method) Part A was heated to 70°C and uniformly dispersed. After heating Part B to 70°C and uniformly dissolving it, it was added to Part A and uniformly dispersed (Part F). Part D, preheated to about 70°C, was added to Part F and emulsified using a homomixer. After cooling to around 40°C, Part C and Part E were added and stirred with a propeller to mix uniformly.

[0132] Example 60 Cream Ingredient Dosage (wt%) -------------------------------------- Part A Phytocompo-PP (Nippon Fine Chemical) 1.0 Glycerin 5.0 1,3-Butylene Glycol 9.0 Part B Plandool-H (Nippon Fine Chemical) 1.5 Glyceryl Tri(caprylate / caprate) 2.0 Behenyl Alcohol 1.0 Stearyl Alcohol 3.2 Arachidyl Alcohol 1.8 Tsubokusa Extract 0.1 Stearyl Glycyrrhetinate 0.05 Part C Tremoist-SL (Nippon Fine Chemical) 5.0 Glyceryl caprylate 0.3 Xanthan gum 0.1 Part D Purified water in an amount to make a total of 100 Erythritol 0.3 Inulin-SC (Nippon Seika) 0.5 Part E Liposome solution of Example 26 30.0 -------------------------------------- (Preparation method) Part A was heated to 70°C and uniformly dispersed. Part B was heated to 70°C and uniformly dissolved, then added to Part A and uniformly dispersed (Part F). Part D, pre-heated to about 70°C, was added to Part F and emulsified using a homomixer. After cooling to around 40°C, Parts C and E were added and stirred with a propeller to mix uniformly.

[0133] Example 61 Cream Component Blending amount (wt%) -------------------------------------- Part A Phytocompo-PP (Nippon Seika) 1.0 Glycerin 5.0 1,3-Butylene glycol 9.0 Part B Plandool-SUN (Nippon Seika) 0.5 Plandool-ISS (Nippon Seika) 1.0 Plandool-G (Nippon Seika) 1.3 Plandool-H (Nippon Seika) 0.5 Plandool-LG4 (Nippon Seika) 0.1 Ethylhexyl palmitate 2.0 Carnauba wax 0.5 Cetyl palmitate 0.8 Behenyl alcohol 1.0 Cetanol 2.0 γ-Oryzanol 0.3 Part C Carbomer (2% aqueous solution, neutralized with KOH) 3.0 Tremoist-SL (Nippon Seika) 2.0 Phenoxyethanol 0.3 Xanthan gum 0.1 Part D Purified water in an amount to make a total of 100 Xylitol 0.3 Part E Liposome solution of Example 25 30.0 -------------------------------------- (Preparation method) Part A was heated to 70°C and uniformly dispersed. After Part B was heated to 70°C and uniformly dissolved, it was added to Part A and uniformly dispersed (Part F). Part D, which had been preheated to about 70°C, was added to Part F and emulsified using a homomixer. After cooling to around 40°C, Part C and Part E were added and stirred with a propeller to mix uniformly.

[0134] Example 62 Scalp / Hair Treatment That Is Not Rinsed Off Ingredient Blending amount (wt%) -------------------------------------- Part A Neosolue-Aqulio (Nippon Seika) 1.0 Neosolue-DiSM (Nippon Seika) 0.5 Elucaraton DES (Nippon Seika) 0.5 Ethanol 20.0 PEG / PPG-20 / 20 Dimethicone 0.2 PEG-12 Dimethicone 0.4 Part B Triethanolamine 0.1 Purified water in an amount to make a total of 100 (Acrylates / Alkyl Acrylate (C10-30)) Crosspolymer 0.1 Tremoist-TP (Nippon Seika) 0.01 (Acryloyldimethyltaurine Ammonium / VP) Copolymer 0.1 (Acryloyldimethyltaurine Ammonium / Beheneth-25 Methacrylate) Crosspolymer 0.1 Phenoxyethanol 0.2 Part C Inulin-SC (Nippon Seika) 1.0 Ethanol 10.0 t-Flavanone 1.0 Menthol 0.1 Menthyloxypropyl Glyceryl Ether 0.15 Panthenyl Ethyl Ether 0.2 Camphor 0.1 Cordyceps sinensis Extract 1.0 Centella asiatica Extract 1.0 Loquat Leaf Extract 1.0 Capsicum frutescens Extract 0.01 Trehalose 0.2 Cymen-5-ol 0.1 Tocopheryl Acetate 0.05 Purified Water 10.0 Part D Liposome Solution of Example 28 20.0 -------------------------------------- (Preparation Method) Part A was heated to about 50 °C and dissolved. Next, Part B was uniformly dispersed to form a viscous liquid. Then, Parts C and D were added and uniformly mixed.

[0135] Example 63 Hair Tonic Component Dosage (wt%) ------------------------------------- Liposome Solution of Example 25 20.0 Glycerin 2.0 PEG-60 Hydrogenated Castor Oil 0.1 Betaine 0.5 Ethanol 20.0 Menthol 0.1 Neosolue-AquaS (Nippon Seika) 2.0 Orange oil 0.05 Panthenyl ethyl ether 0.2 Glutamic acid 0.1 Polychlorinated dimethyl methylene piperidinium solution 0.1 Carrot extract 1.0 Clara extract 2.0 Chlorella extract 1.0 Trehalose 0.5 Nicotinamide 1.0 Piroctone olamine 0.1 Dipotassium glycyrrhizinate 0.05 Purified water in an amount to make 100 in total ------------------------------------- (Preparation method) Weighed all the ingredients into a container and stirred with a paddle at room temperature to make a uniform liquid.

[0136] Example 64 Hair growth agent Ingredient Blending amount (wt%) ------------------------------------- Liposome solution of Example 26 20.0 Glycerin 2.0 PEG-60 hydrogenated castor oil 0.1 Betaine 0.5 Neosolue-AquaS (Nippon Fine Chemical) 2.0 Neosolue-Aqulio (Nippon Fine Chemical) 1.0 Inulin-SC (Nippon Fine Chemical) 1.0 Ethanol 10.0 t-Flavanone 1.0 Menthol 0.1 Menthy glyceryl ether 0.15 Panthenyl ethyl ether 0.2 Camphor 0.1 Cordyceps sinensis extract 1.0 Assembly extract 1.0 Loquat leaf extract 1.0 Hydrolyzed hyaluronic acid 0.02 Sodium cyclic lysophosphatidic acid 0.001 Capsicum extract 0.01 Betaine 0.3 Trehalose 0.2 Cymen-5-ol 0.1 Tocopheryl acetate 0.05 β-Glycyrrhetinic acid 0.05 Purified water (amount to make a total of 100) ------------------------------------- (Preparation method) All ingredients were weighed into a container and stirred with a paddle at room temperature to form a homogeneous liquid.

[0137] Example 65 Lotion Component Amount (wt%) -------------------------------------- Part A Liposome solution of Example 26 50.0 Part B Allantoin 0.2 Carbomer (2% aqueous solution, neutralized with KOH) 3.0 Salicylic acid 0.1 Phenoxyethanol 0.5 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part B was added to Part A and stirred with a paddle until homogenous.

[0138] Example 66 Lotion Component Amount (wt%) -------------------------------------- Part A Liposome solution of Example 23 30.0 Part B Nicotinamide 2.0 Carbomer (2% aqueous solution, neutralized with KOH) 3.0 Glycyrrhizic acid 2K 0.05 Phenoxyethanol 0.5 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part B was added to Part A and stirred with a paddle until homogenous.

[0139] Example 67 Lotion Component Amount (wt%) -------------------------------------- Part A Liposome solution of Example 23 30.0 Part B Magnesium Ascorbyl Phosphate 2.0 Xanthan gum (3% aqueous solution) 10.0 Glycyrrhizic acid 2K 0.15 Phenoxyethanol 0.5 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part B was added to Part A and stirred with a paddle until homogenous.

[0140] Example 68 Lotion Component Amount (wt%) -------------------------------------- Part A Liposome solution of Example 28 50.0 Part B Nicotinamide 5.0 Carbomer (2% aqueous solution, neutralized with KOH) 3.0 Tranexamic acid (Nippon Fine Chemical) 2.0 Phytic acid 0.3 Phenoxyethanol 0.3 Caffeine 0.1 Acetylated hyaluronic acid 0.1 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) The B part was added to the A part and stirred with a paddle until uniform.

[0141] Example 69 Lotion Ingredient Blending amount (wt%) -------------------------------------- Part A Liposome solution of Example 23 40.0 Part B Nicotinamide 5.0 3-O-Ethylascorbic acid (Nippon Fine Chemical) 2.0 Acetylated hyaluronic acid 0.05 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) The B part was added to the A part and stirred with a paddle until uniform.

[0142] Example 70 Lotion Ingredient Blending amount (wt%) -------------------------------------- Part A Liposome solution of Example 28 50.0 Part B Placenta extract 0.01 Glycyrrhizic acid 2K 0.1 Carbomer (2% aqueous solution, neutralized with KOH) 3.0 Tranexamic acid (Nippon Fine Chemical) 2.0 Phytic acid 0.3 Phenoxyethanol 0.3 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part B was added to Part A and stirred with a paddle until homogenous.

[0143] Example 71 Lotion Component Amount (wt%) -------------------------------------- Part A Liposome solution of Example 28 50.0 Part B 3-O-Ethyl ascorbic acid (Nippon Fine Chemical) 0.1 Isopropylmethylphenol 0.1 Tranexamic acid (Nippon Fine Chemical) 2.0 Phenoxyethanol 0.3 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part B was added to Part A and stirred with a paddle until homogenous.

[0144] Example 72 Lotion Component Amount (wt%) -------------------------------------- Part A Liposome solution of Example 28 50.0 Part B 3-O-Ethyl ascorbic acid (Nippon Fine Chemical) 1.5 Isopropylmethylphenol 0.1 Ascorbic Acid 5.0 Tranexamic acid (Nippon Fine Chemical) 1.0 Allantoin 0.1 Urea 1.0 Cyanocobalamin 0.001 Trisodium Ascorbyl Palmitate Phosphate 0.1 Phenoxyethanol 0.3 1,2-Pentanediol 1.5 Ethanol 8.0 Citric acid (appropriate amount) Sodium citrate 0.2 Purified water (amount to make a total of 100) -------------------------------------- (Preparation method) Part B was added to Part A and stirred with a paddle until homogenous. The amount of citric acid added was adjusted to a pH of around 4.5.

[0145] Example 73 Cream Component Amount (wt%) -------------------------------------- Part A Isononyl isononanoate 2.0 Tocotrienol 0.02 Diglyceryl stearate 1.5 Glyceryl stearate 1.0 Polysorbate-20 0.1 PEG-60 Hydrogenated Castor Oil 0.8 Pyridoxine Tripalmitate 1.0 Pyridoxine Dipalmitate 1.0 Retinol Palmitate 0.005 Eucalyptus oil 0.2 Argania spinosa kernel oil 0.01 Peppermint oil 0.4 Aminocaproic acid 0.2 Pantothenyl alcohol 0.2 Tocopheryl acetate 0.02 Stearyl glycyrrhetinate 0.05 Part B Carbomer 0.01 (Acrylates / C10-30 alkyl acrylate) crosspolymer 0.01 Xanthan gum 0.05 Sodium hydroxide 0.01 Part C Purified water in an amount to make a total of 100 Inulin-SC (Nippon Seika) 0.8 Part D Liposome solution of Example 25 30.0 -------------------------------------- (Preparation method) Part A was heated to 70°C and dissolved uniformly. Part C, which had been heated to 70°C in advance, was added to Part A and emulsified using a homomixer. After cooling to around 40°C, Part B and Part D were added and stirred with a propeller to mix uniformly.

[0146] Example 74 Cream Component Blending amount (wt%) -------------------------------------- Part A Petrolatum 3.0 Glyceryl stearate 1.0 Polysorbate-20 0.1 PEG-60 hydrogenated castor oil 0.8 Jojoba oil 2.0 Macadamia nut oil 1.0 Meadowfoam oil 1.0 Cetanol 3.0 Stearyl glycyrrhetinate 0.2 Tocopherol nicotinate 0.1 Part B Carbomer 0.01 (Acrylates / Alkyl acrylate (C10-30)) Crosspolymer 0.01 Xanthan gum 0.05 Sodium hydroxide 0.01 Part C Purified water in an amount to make a total of 100 Arbutin (Nippon Seika) 6.0 Inulin-SC (Nippon Seika) 0.8 Part D Liposome solution of Example 25: 30.0 -------------------------------------- (Preparation method) Part A was heated to 70°C and dissolved uniformly. Part C, which had been preheated to 70°C, was added to Part A, and emulsification was carried out using a homomixer. After cooling to around 40°C, Part B and Part D were added and stirred with a propeller to mix uniformly.

[0147] Lotion of Example 75 Components Blending amount (wt%) -------------------------------------- Part A Liposome solution of Example 23: 30.0 Part B Sorbitol: 1.0 Xanthan gum (3% aqueous solution): 10.0 Dipotassium glycyrrhizinate: 0.15 Sodium tocopheryl phosphate: 1.0 Phenoxyethanol: 0.5 Purified water Amount to make a total of 100 -------------------------------------- (Preparation method) Part B was added to Part A and stirred with a paddle until uniform.

[0148] Liposome solution of Example 76 Components Blending amount (wt%) -------------------------------------- Part A Complex of Example 19: 0.5 1,3-Butylene glycol: 5.0 Part B Phenoxyethanol: 0.3 Purified water Amount to make a total of 100 Part C Hydrogenated soybean phospholipid (PC content 80%): 0.2 1,3 - Butylene glycol 5.0 Part D Purified water 30.0 -------------------------------------- (Preparation method) Parts A to D were each heated at about 80°C. While stirring with a homomixer (5000 rpm), part A was added to part B and part C was added to part D, and they were mixed after cooling.

[0149] Example 77 Liposome solution Component Dosage (wt%) -------------------------------------- Part A Complex of Example 12 0.2 1,3 - Butylene glycol 5.0 1,2 - Pentanediol 1.0 Part B Methylparaben 0.1 Purified water An amount to make a total of 100 Part C Hydrogenated soy phosphatidylcholine (PC content 70% or more) 0.2 Phytosterol 0.03 Dipropylene glycol 5.0 Part D Purified water 40.0 Part E Lactic acid Appropriate amount -------------------------------------- (Preparation method) Parts A to D were each heated at about 80°C. While stirring with a homomixer (5000 rpm), part A was added to part B and part C was added to part D, and they were mixed after cooling. Then, the pH was adjusted to 5 with lactic acid in part E.

[0150] Example 78 Liposome solution Component Dosage (wt%) -------------------------------------- Part A Complex of Example 12 0.2 1,3-butylene glycol 5.0 1,2-Pentanediol 1.0 Part B Caprylyl Glycol 0.4 Phenoxyethanol 0.2 Purified water (amount to make a total of 100) C part Hydrogenated soybean phospholipid (PC content 70% or more) 0.2 Dipropylene Glycol 5.0 D part Purified water 40.0 Part E Lactic acid appropriate amount -------------------------------------- (Preparation method) Parts A to D were each heated to approximately 80°C. While stirring with a propeller (800 rpm), part A was added to part B, and part C to part D. After cooling, they were mixed. Then, the pH was adjusted to 5 with part E of lactic acid.

[0151] Example 79 Liposome solution Component Amount (wt%) -------------------------------------- Part A Complex of Example 19 0.2 1,3-butylene glycol 5.0 Stearyl Glycyrrhetinate 0.1 Part B Glyceryl Caprylate 0.4 Clove extract 0.3 Purified water (amount to make a total of 100) C part Citric acid 0.2 Sodium citrate (appropriate amount) -------------------------------------- (Preparation method) Part A and Part B were each heated to about 80°C. While stirring with a homomixer (4000 rpm), Part A was added to Part B and then cooled. Thereafter, the pH was adjusted to 5.5 with Part C.

[0152] Example 80 Liposome solution Component Dosage (wt%) -------------------------------------- Part A Complex of Example 19 0.2 1,3-Butylene glycol 5.0 Stearyl glycyrrhetinate 0.1 Part B Glyceryl caprylate 0.5 Purified water An amount to make 100 in total Part C Polyoxyethylene oleyl ether 1.0 Schizophyllan 0.02 Glycosyl trehalose 1.0 Hydrolyzed hydrogenated starch 0.5 Tea leaf extract 0.1 Rosemary leaf extract 0.1 Lemon fruit extract 0.1 Soybean seed extract 0.1 Methyl gluceth-20 0.3 -------------------------------------- (Preparation method) Part A and Part B were each heated to about 80°C. While stirring with a homomixer (4000 rpm), Part A was added to Part B and then cooled. Thereafter, Part C was added and mixed until uniform.

[0153] Example 81 Cream Component Dosage (wt%) ------------------------------------- Part A Sorbitan stearate 0.5 Sucrose cocoate 1.0 Sucrose stearate 1.0 Glyceryl stearate 1.5 Glycerin 5.0 1,3-Butylene glycol 5.0 Isopropylmethylphenol 0.1 Part B Ceteareth-20 0.5 Decyl glucoside 0.3 Polyglyceryl-10 stearate 0.3 PEG-20 hydrogenated castor oil 1.0 PEG-60 hydrogenated castor oil 1.0 PEG-10 dimethicone 0.5 Plandool-MAS (Nippon Seika) 1.0 LUSPLAN PI-DA (Nippon Seika) 1.0 Shea butter 1.0 Macadamia nut oil 0.1 Tocopheryl acetate 0.1 Stearyl glycyrrhetinate 0.1 Ascorbyl tetrahexyldecanoate 0.5 Tocopheryl nicotinate 0.1 Lanolin 1.0 Liquid lanolin 0.5 Hard lanolin 0.5 Camellia oil 0.1 Sunflower seed oil 0.1 Jojoba seed oil 0.1 Rose hip oil 0.1 Komenuka oil 0.1 Mineral oil 0.1 Soybean oil 0.1 Argania spinosa kernel oil 0.1 Almond oil 0.1 Cetearyl alcohol 2.0 Sunflower seed wax 2.0 Part C Purified water in an amount to make a total of 100 Part D 2% Carbomer neutralized with KOH 5.0 Pentylene glycol 1.0 Lavender flower extract 0.01 Torenia fournieri flower extract 0.01 Ajuga decumbens extract 0.01 ε-Aminocaproic acid 0.1 Allantoin 0.1 Salicylic acid 0.2 Eucalyptus oil 0.2 Menthol 0.2 dl-Camphor 0.5 Purified water 5.0 Part E Liposome solution of Example 24 30.0 ------------------------------------- (Preparation method) Parts A, B, and C were each mixed, heated to about 70 °C, and uniformly dissolved and dispersed. While stirring part A with a homodisper, part B was added, and then part C was added and emulsified. After cooling to about 50 °C, parts D and E were added while stirring (pH about 6.0, viscosity about 70,000 mPa·s).

[0154] Example 82 Cream Ingredient Dosage (wt%) ------------------------------------- Part A Phytocompo-PP (Nippon Seika) 0.5 Glycerin 5.0 1,3-Butylene glycol 5.0 Part B Polyglyceryl-2 isostearate 0.3 Polyglyceryl-10 oleate 0.7 Polyglyceryl-10 stearate 0.5 Glyceryl stearate (SE) 0.8 Sorbitan oleate 0.3 Petrolatum 3.0 Sugar Squalan 2.0 Dodecane 1.0 Shea Butter 1.0 Macadamia Nut Oil 0.1 Almond Oil 0.1 Camellia Oil 0.1 Sunflower Seed Oil 0.1 Jojoba Seed Oil 0.1 Rose Hip Oil 0.1 Ceramide 1 0.01 Ceramide 2 0.01 Ceramide 3 0.1 Ceramide 4 0.02 Ceramide 5 0.015 Ceramide 6 0.03 Rice Bran Wax 0.3 Complex of Example 16 0.5 Part C Purified Water in an amount to total 100 Part D Carbomer (2% KOH neutralized product) 5.0 Pentylene Glycol 1.0 Lavender Flower Extract 0.01 Peony Flower Extract 0.01 ε-Aminocaproic Acid 0.1 Allantoin 0.1 Methyl Gluceth-10 0.5 Purified Water 5.0 ------------------------------------- (Preparation Method) Parts A, B, and C were each mixed, heated to about 70°C, and uniformly dissolved and dispersed. While stirring Part A with a homodisper, Part B was added, and then Part C was added and emulsified. After cooling to about 50°C, Part D was added while stirring (pH about 5.5, viscosity about 55,000 mPa·s).

[0155] Example 83 Body Shampoo Ingredient Blending Amount (wt%) ------------------------------------ Complex of Example 19 1.0 Cocoyl methyl taurine Na 10.0 Lauroyl hydrolyzed silk Na 6.0 Lauroyl methyl alanine Na 10.0 Coco amphoacetate Na (30%) 4.0 Cocamidopropyl betaine (30%) 10.0 Olefin (C14 - 16) sulfonic acid Na 5.0 Polyquaternium - 10 1.3 Guar hydroxypropyltrimonium chloride 0.8 Resveratrol 0.05 Neosolue - AquaS (Nippon Seika) 0.6 Sodium acetyl hyaluronate 0.02 Cocamide DEA 3.0 Glycerin 5.0 Sodium benzoate 0.2 Phenoxyethanol 0.5 Purified water in an amount to make a total of 100 ------------------------------------ (Preparation method) All components were heated at about 80 °C and stirred and mixed uniformly (pH about 5, viscosity about 8,000 mPa·s).

[0156] Example 84 Non - silicone shampoo Components Dosage (wt%) ------------------------------------ Part A Complex of Example 19 0.5 Plandool - LG3 (Nippon Seika) 0.03 Neosolue - AquaS (Nippon Seika) 0.1 Tremoist - SL (Nippon Seika) 0.5 Sodium laures - 4 - carboxylate (28%) 10.0 Lauric - 6 - carboxylic acid 4.0 Cocamidopropyl betaine (30%) 5.0 Sodium lauroyl methyl alaninate (30%) 5.0 Cocamidomethyl MEA 3.0 PEG-7 glyceryl cocoate 5.0 Sodium hydroxide 1% solution appropriate amount Polyquaternium-10 0.8 Glycol distearate 0.5 Polyquaternium-7 0.5 EDTA-2Na 0.05 Methylparaben 0.2 Phenoxyethanol 0.4 Purified water Amount to make 100 in total ------------------------------------ (Preparation method) All components were heated at about 80 °C and stirred and mixed uniformly (pH about 5, viscosity about 3,000 mPa·s).

[0157] Example 85 Non-silicone hair treatment Component Blending amount (wt%) -------------------------------------- Part A Plandool-LG2 (Nippon Seika) 0.5 Propanediol 3.0 Behenamidopropyl dimethylamine 1.5 Cetanol 2.2 Stearyl alcohol 0.8 Ascorbyl tetrahexyldecanoate 1.0 Hydrogenated rapeseed oil alcohol 0.5 Neopentyl glycol diethylhexanoate 0.8 Jojoba seed oil 1.0 Macadamia nut oil 0.4 Glyceryl stearate 0.4 Cocamid MEA 0.8 Caesalpinia spinosa hydroxypropyltrimonium chloride 0.1 rice bran wax 0.5 glycerin 1.0 Part B phenoxyethanol 0.4 Tremoist-SL (Nippon Seika) 1.0 lactic acid 0.4 purified water in an amount to make 100 in total Part C liposome solution of Example 78 1.0 methyl heptyl laurate 1.0 polyquaternium-7 0.15 PPG-3 benzyl ether myristate 0.5 -------------------------------------- (Preparation method) Part A was uniformly mixed at about 80°C. Separately taken Part B was heated to about 60°C and made uniform. Part B was gradually added to Part A while stirring, and after uniformly mixing, it was cooled to about 50°C. Further, Part C was added and uniformly mixed (pH about 4, viscosity about 65,000 mPa·s).

[0158] Example 86 Non-rinse Hair Treatment Ingredient Blending amount (wt%) -------------------------------------- Part A Neosolue-Aqulio (Nippon Seika) 1.0 ethylhexyl sebacate (Nippon Seika) 1.0 cetrimonium chloride (25%) 2.8 lanolin wax 1.0 PPG-3 caprylyl ether 1.0 methyl heptyl myristate 0.5 cetyl ethylhexanoate 0.3 isononyl isononanoate 0.3 tridecyl neopentanoate 0.5 Myristyl isooctanoate 0.3 Neopentyl glycol diethylhexanoate 0.3 Isobutyl isostearate 0.3 Glyceryl triisostearate 0.3 Trimethylolpropane triisostearate 0.3 Broccoli seed oil 0.3 Sphinganine 0.01 Glycerin 1.0 Part B Purified water in an amount to make a total of 100 Phenoxyethanol 0.5 Part C Liposome solution of Example 24 30.0 Polyquaternium-6 0.6 Polyquaternium-7 0.4 Polyquaternium-39 0.5 Part D Carrageenan 0.1 Hydroxypropyl chitosan 0.05 Hydroxypropyl methylcellulose 0.1 Tuberose polysaccharide 0.1 Cationized guar gum 0.05 Hydrolyzed keratin (molecular weight 2000) 1.0 Purified water 20.0 -------------------------------------- (Preparation method) Part B was heated to about 80 °C and dissolved. Next, Part A was heated to about 80 °C and dissolved. Part D was dispersed to form a viscous liquid. Part A was added to Part B with stirring, and after uniformly mixing, it was cooled to about 40 °C. Then, Part C and Part D were added and uniformly mixed (pH about 5.5, viscosity about 65,000 mPa·s).

[0159] Example 87 Liposome solution Component Dosage (wt%) -------------------------------------- Part A Complex of Example 18 1.0 1,3 - Butylene glycol 9.0 1,2 - Pentanediol 2.0 Bactiol 0.2 Part B Ethylhexylglycerin 0.5 Nicotinamide mononucleotide 0.1 Cytidyl phosphate disodium 0.1 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0160] Example 88 Liposome solution Component Blending amount (wt%) -------------------------------------- Part A Complex of Example 18 1.0 1,3 - Butylene glycol 9.0 1,2 - Pentanediol 2.0 Adenosine 0.1 Part B Cloves extract 0.3 Artemisia princeps extract 0.2 Nicotinamide mononucleotide 0.1 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0161] Example 89 Liposome solution Component Blending amount (wt%) -------------------------------------- Part A Complex of Example 11 1.0 1,3 - Butylene glycol 9.0 Part B Glucosyl trehalose 0.2 Polysaccharide produced by Alcaligenes 0.1 Artemisia princeps extract 0.4 Houttuynia cordata extract 0.2 Fermented liquid of Lactobacillus / apple juice 0.3 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0162] Liposome solution of Example 90 Components Dosage (wt%) -------------------------------------- Part A Complex of Example 11 1.0 1,3 - Butylene glycol 9.0 Part B Inulin - SC 0.5 Artemisia princeps extract 0.4 Parsnip root extract 0.2 Fermented liquid of Lactobacillus / apple juice 0.3 Purified water in an amount to make a total of 100 -------------------------------------- (Preparation method) Part A and Part B were each heated at about 80°C. While stirring with a homomixer (5000 rpm), Part A was added to Part B and then cooled.

[0163] Example 91 Sunscreen Component Blending amount (mass%) ------------------------------------- Part A Dimethicone (2cs) 25.0 Phenyltrimethicone 3.0 (Dimethicone / PEG-10 / 15) Crosspolymer 5.0 PEG-9 Polydimethylsiloxyethyl Dimethicone 0.4 STR-100A-LP (Sakai Chemical Industry) 10.0 FINEX-33W-LP2 (Sakai Chemical Industry) 5.0 MiyoFEEL SA-SB-N1 (Miyoshi Kasei) 1.0 MiyoNAT CAI-TA-N1 (Miyoshi Kasei) 1.0 Cerium Oxide 1.5 Alumina 3.0 RonaFlair Boroneige SF-6 (Merck) 1.0 Tocopherol 0.2 Part B 1,3-Butylene Glycol 10.0 Phenoxyethanol 0.5 Purified water An amount that totals 100 Part C Liposome solution of Example 22 30.0 ------------------------------------- (Preparation method) Part A was uniformly stirred while heating at 80°C. Part B was added to Part A while stirring. After cooling, Part C was added and stirred until uniform.

[0164] Example 92 Sunscreen Component Blending amount (mass%) ------------------------------------- Part A Dimethicone (50cs) 15.0 Dimethicone (100 cs) 10.0 (Dimethicone / PEG-10 / 15) Crosspolymer 7.0 PEG-9 Polydimethylsiloxyethyl Dimethicone 1.5 STR-100A-LP (Sakai Chemical Industry) 11.0 FINEX-33W-LP2 (Sakai Chemical Industry) 6.0 MiyoFEEL SA-SB-N1 (Miyoshi Kasei) 1.5 MiyoNAT CAI-TA-N1 (Miyoshi Kasei) 1.5 Alumina 3.0 RonaFlair Boroneige SF-6 (Merck) 1.0 Composite of Example 11 0.8 Part B 1,3-Butylene Glycol 4.0 Phenoxyethanol 0.3 Purified water in an amount to make a total of 100 ------------------------------------- (Preparation Method) Part A was uniformly stirred while heating at 80°C. Part B was added to Part A while stirring.

[0165] Example 93 Sunscreen Component Blending Amount (mass%) ------------------------------------- Part A Cyclomethicone 25.0 Dimethicone (50 cs) 15.0 Polyglyceryl-2 Diisostearate 3.0 PEG-10 Dimethicone 5.0 Ethylhexyl Methoxycinnamate 7.0 Bis-Ethylhexyl Oxy-Phenol Methoxyphenyl Triazine 1.0 Octocrylene 5.0 Diisopropyl Sebacate (Nippon Fine Chemical) 10.0 Tocopherol 0.2 Section B 1,3-Butylene glycol 6.0 Ethanol 3.0 Composite of Example 19 0.5 Purified water in an amount to make 100 in total ------------------------------------- (Preparation method) Part A was uniformly stirred while heating at 80°C. Part B was added to Part A while stirring.

[0166] Example 94 Sunscreen Components Dosage (mass%) ------------------------------------- Part A Cetyl ethylhexanoate 15.0 Dimethicone (2 cs) 6.0 (Sodium acrylate / sodium acryloyldimethyltaurate) copolymer 1.5 Part B STR-100W(G) (Sakai Chemical Industry) 10.0 FINEX-33W (Sakai Chemical Industry) 5.0 1,3-Butylene glycol 7.0 Ethanol 7.0 Purified water in an amount to make 100 in total Part C Liposome solution of Example 24 20.0 ------------------------------------- (Preparation method) Part A was uniformly stirred while heating at 80°C. Part B was added to Part A while stirring to emulsify. After cooling to 40°C, Part C was added and stirred until uniform. ⏎

[0167] In the above formulation, the details of the components described by the product name are as follows. 〇Inulin-SC: Inulin 〇LUSPLAN PI-DA: Di(isostearyl / phytosteryl) dimer dilinoleate 〇LUSPLAN SR-DM4: Dimer dilinoleyl dimer dilinoleate, glyceryl tri(caprylate / caprate) 〇Neosolue-Aqulio: Bis(ethoxydiglycol) cyclohexanedicarboxylate 〇Neosolue-AquaS: Polyglyceryl-10 (eicosanedioate / tetradecanedioate), glycerin 〇Neosolue-DiSM: Diisostearyl malate 〇Phytocompo-PP: Hydrogenated lecithin, phytosterol 〇Phytocompo-C: Hydrogenated lecithin, phytosterol, ceramide 2, ceramide 3, ceramide 6II 〇Plandool-H: (Phytosteryl / isostearyl / cetyl / stearyl / behenyl) dimer dilinoleate 〇Plandool-G: Bis(behenyl / isostearyl / phytosteryl) dimer dilinoleyl dimer dilinoleate 〇Plandool-ISS: Phytosteryl isostearate 〇Plandool-SUN: Phytosteryl sunflower seed oil fatty acid 〇Plandool-MAS: Phytosteryl macadamia nut oil fatty acid 〇Plandool-LG1: Di(phytosteryl / octyldodecyl / behenyl) lauroyl glutamate 〇Plandool-LG2: Di(phytosteryl / octyldodecyl) lauroyl glutamate 〇Plandool-LG3: Di(phytosteryl / octyldodecyl / behenyl) lauroyl glutamate 〇Plandool-LG4: Di(phytosteryl / octyldodecyl / behenyl) lauroyl glutamate 〇PrimeLipid ALPA: Lysophosphatidic acid, lysophosphatidylcholine, glycerin, water, Al hydroxide 〇PrimeLipid PI: Hydrogenated Lecithin, Phytosterol, Lecithin, Tocopherol 〇Tremoist-TP: Jellyfish polysaccharide 〇Tremoist-SL: Aqueous solution of jellyfish polysaccharide (1%) 〇Erucalactone DES: γ-Docosalactone, Diethyl Sebacate 〇MiyoFEEL SA-SB-N1: Silica, Dimethicone 〇MiyoNAT CAI-TA-N1: Talc, Sodium Cocoyl Glutamate 2Na, Aluminum Hydroxide 〇FINEX-33W-LP2: Zinc Oxide, Hydrous Silica, Hydrogen Dimethicone 〇STR-100A-LP: Titanium Oxide, Hydrous Silica, Aluminum Hydroxide, Hydrogen Dimethicone 〇RonaFlair Boroneige SF-6: Boron Nitride 〇STR-100W(G): Titanium Oxide, Hydrous Silica 〇FINEX-33W: Zinc Oxide, Hydrous Silica

Claims

1. The following components (A) and (B): (A) Phospholipid (B) Sphingosines A positively charged complex for preparing liposomes, obtained by removing an organic solvent from a solution in which the components (A) and (B) are uniformly dissolved in the organic solvent to simultaneously precipitate the components (A) and (B).

2. The complex for preparing liposomes according to Claim 1, wherein the phospholipid as the component (A) is lecithin having a phosphatidylcholine content of 60% by mass or more.

3. In the complex of Claim 2, further containing sterols as the component (C), and removing the organic solvent from a solution in which the components (A) to (C) are uniformly dissolved in the organic solvent to simultaneously precipitate the components (A) to (C), a positively charged complex for preparing liposomes.

4. In the complex according to any one of Claims 1 to 3, further containing an organic acid as the component (D), and removing the organic solvent from a solution in which the components (A) to (D) are uniformly dissolved in the organic solvent to simultaneously precipitate the components (A) to (D), a positively charged complex for preparing liposomes.

5. Liposomes obtained by dispersing the complex for preparing liposomes according to any one of Claims 1 to 3 in water.

6. Liposomes obtained by dispersing the complex for preparing liposomes according to Claim 4 in water.

7. A cosmetic or an external preparation for skin containing the complex for preparing liposomes according to any one of Claims 1 to 3.

8. A cosmetic or an external preparation for skin containing the complex for preparing liposomes according to Claim 4.

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