Extraction device and extraction method

Abstract

To provide an extraction device capable of appropriately extracting content from an object.SOLUTION: An extraction device 1 includes: a tubular capillary 20; and a suction device 60 which applies negative pressure to an inside of the capillary 20 and sucks a content C of an object T from a tip E of the capillary 20.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present disclosure relates to a brewing device and a brewing method. [Background technology]

[0002] Patent Document 1 discloses a manipulation system that can maintain favorable contact between an electrode and an active material. The manipulation system in Patent Document 1 includes a capillary that attracts and holds the active material (target). The capillary can fix the position of the target and prevent the target from moving. [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent Publication No. 2021-084209 Summary of the Invention [Problem to be solved by the invention]

[0004] In a system for manipulating an object such as that described in Patent Document 1, the object may be a minute object such as a cell cluster. A cell cluster is composed of multiple types of cells, and by extracting and analyzing some cells from the cell cluster, the behavior of the cell cluster can be more accurately understood. Therefore, in a system for manipulating an object, there is a demand not only for evaluating the object while holding it, but also for extracting and analyzing the contents of the object.

[0005] The present disclosure aims to provide an extraction device that can appropriately extract the contents of an object. [Means for solving the problem]

[0006] An extraction device according to one aspect of the present disclosure includes a tubular capillary and a suction device that creates a negative pressure inside the capillary and sucks the contents of an object from the tip of the capillary.

[0007] According to this, the contents of the object are sucked by the suction device and held inside the capillary, and therefore the extraction device can properly extract the contents of the object.

[0008] In addition, in an extraction apparatus according to one aspect of the present disclosure, the suction device further includes a suction pump, a first pipe connecting the suction pump and the capillary, a hollow tank disposed in the first pipe, and a first valve disposed in the first pipe between the tank and the capillary and configured to open and close the first pipe, wherein the first valve is closed to cause the suction pump to create a negative pressure in the tank, and then the first valve is opened to cause the suction pump to create a negative pressure inside the capillary.

[0009] With this, when suction of the contents begins, the negative pressure in the tank acts on the inside of the capillary early, thereby stabilizing the suction force acting on the object compared to when the suction device does not have a tank.

[0010] In addition, in an extraction device according to one aspect of the present disclosure, the suction device further includes a pressure pump, a second pipe connecting the pressure pump and the capillary, and a second valve disposed on the second pipe for opening and closing the second pipe, and after the pressure pump applies pressure to the inside of the capillary with the first valve in a closed state and the second valve in an open state, the pressure inside the capillary is reduced by the suction pump with the first valve in an open state and the second valve in a closed state.

[0011] This allows the contents of the target object to be removed from the inside of the capillary before the contents of the target object are aspirated, thereby preventing the extraction device from aspirating anything other than the contents of the target object into the inside of the capillary.

[0012] In addition, in an extraction apparatus according to one aspect of the present disclosure, the suction device further includes a third pipe having a first end connected between the suction pump and the tank in the first pipe and a second end open to the atmosphere, and a third valve disposed in the third pipe for opening and closing the third pipe, and the first valve and the third valve are opened to create a negative pressure inside the capillary using the suction pump.

[0013] According to this, when the suction pump sucks the contents of the object, it sucks in the atmosphere through the third pipe. Therefore, the suction force acting on the contents of the object is suppressed, and the entire object, including the contents and the outer shell, can be prevented from being sucked into the capillary prematurely. Therefore, the extraction device can accurately suck in the contents of the object.

[0014] In the extraction device according to one aspect of the present disclosure, the suction pump is a vacuum pump.

[0015] This allows the suction pump to reliably suck up the contents of the object.

[0016] In the extraction device according to an aspect of the present disclosure, the object is a cell mass.

[0017] This allows the extractor to aspirate the contents of the cell cluster, and therefore the extractor can provide the contents of the cell cluster that are relatively less affected by external direct influences as an analysis sample.

[0018] In addition, the extraction device according to one aspect of the present disclosure further includes a moving device that moves the capillary, the moving device moves the capillary so that the tip of the capillary is positioned inside the object, and the suction device suctions the contents while the tip of the capillary is positioned inside the object.

[0019] This allows the extraction device to reliably suck up the contents of the object.

[0020] Furthermore, the extraction device according to one aspect of the present disclosure further includes a hardness deriving device that derives the hardness of the object based on the amount of the content sucked into the inside of the capillary by the suction device.

[0021] This allows the extraction device to derive the hardness of the object when the contents of the object are aspirated.

[0022] In addition, in the extraction device according to one aspect of the present disclosure, the tip portion of the capillary has an outer diameter that decreases from the base end side to the tip end side of the capillary.

[0023] According to this, the tip of the capillary pierces the outer shell of the object, so that the tip of the capillary is reliably positioned inside the object, and therefore the extraction device can reliably aspirate the contents of the object.

[0024] Furthermore, an extraction method according to one aspect of the present disclosure includes the steps of moving a tubular capillary to position the tip of the capillary inside an object, and creating negative pressure inside the capillary with a suction device while the tip of the capillary is positioned inside the object, and aspirating the contents of the object from the tip of the capillary.

[0025] According to this, the contents of the object are sucked by the suction device and held inside the capillary, thereby enabling the contents of the object to be appropriately extracted. [Brief explanation of the drawings]

[0026] [Figure 1] FIG. 1 is a diagram illustrating a configuration of an extraction device according to an embodiment of the present disclosure. [Figure 2] FIG. 2 is a cross-sectional view schematically showing a capillary. [Figure 3] FIG. 3 is a diagram showing the configuration of the suction device. [Figure 4] FIG. 4 is a diagram showing the state of the object when the contents are sucked. [Figure 5]FIG. 5 is a flowchart showing the process executed by the control device when the extractor aspirates the contents. [Figure 6] FIG. 6 is a diagram showing the configuration of a suction device according to a modified example of the embodiment of the present disclosure. DETAILED DESCRIPTION OF THE INVENTION

[0027] Hereinafter, embodiments of the present disclosure will be described with reference to the drawings, but the present disclosure is not limited thereto. The components of each embodiment described below can be combined as appropriate. In addition, some components may not be used.

[0028] In the following description, the Z direction is the up-down direction of the extraction device 1, the X direction is the left-right direction of the extraction device 1, and the Y direction is the front-rear direction of the extraction device 1. The X direction, Y direction, and Z direction are perpendicular to each other. Note that the X, Y, and Z directions are merely examples, and the present disclosure is not limited to these directions.

[0029] FIG. 1 is a diagram showing the configuration of an extraction device 1 according to an embodiment of the present disclosure. The extraction device 1 is a device for extracting the contents of a target object T. The target object T is relatively small, such as a cell mass. The target object T may also be an elastic body such as a silicone rubber bead, or a viscoelastic body such as a spheroid or an organoid. The contents of the target object T are mainly used as an analysis sample.

[0030] The extraction device 1 includes a base 10, a capillary 20, a holding member 30, a moving device 40, an imaging device 50, a suction device 60, and a control device .

[0031] 2 is a cross-sectional view schematically showing the capillary 20. The capillary 20 is a straight, hollow tube. Both ends of the capillary 20 are open. The capillary 20 is made of, for example, glass. Note that the material of the capillary 20 is not limited to glass, and may be an electrically insulating material or a conductive material.

[0032] The capillary 20 has a main body 21 and a tip portion 22 that is continuous with the main body 21 on the tip E side. The outer diameter and inner diameter of the main body 21 are both approximately constant. The outer diameter and inner diameter of the tip portion 22 decrease from the base end B side of the capillary 20 toward the tip E side. The inner diameter of the tip E of the tip portion 22 is between approximately 10 μm and 150 μm. It goes without saying that the inner diameter of the tip E is not limited to the above size.

[0033] 1 holds the capillary 20. The holding member 30 grips the main body 21 of the capillary 20.

[0034] The moving device 40 is disposed on the base 10 and moves the holding member 30. The moving device 40 includes an XY table 41 that moves the holding member 30 in both the X and Y directions relative to the base 10, and a first actuator 42 that moves the XY table 41. The XY table 41 moves the holding member 30 in both the X and Y directions relative to the base 10.

[0035] Furthermore, the moving device 40 moves the holding member 30 along the Z direction. The moving device 40 includes a Z table 43 that moves the holding member 30 along the Z direction relative to the base 10, and a second actuator 44 that moves the Z table 43.

[0036] The imaging device 50 captures an image of the object T. The imaging device 50 also captures images of the object T and the capillary 20 held by the holding member 30. The imaging device 50 includes a first imaging section 51 and a second imaging section 52.

[0037] The first imaging unit 51 and the second imaging unit 52 capture images of the object T and the capillary 20 from directions that intersect with each other (for example, directions that are perpendicular to each other). The first imaging unit 51 captures images of the object T and the capillary 20, for example, along the Z direction. The second imaging unit 52 captures images of the object T and the capillary 20, for example, along the X direction. It goes without saying that the directions in which the first imaging unit 51 and the second imaging unit 52 capture images of the object T and the capillary 20 are not limited to the above directions.

[0038] The first imaging unit 51 and the second imaging unit 52 are configured similarly, and each has an optical system and an imaging element. The optical system has multiple lenses and the focal length is adjustable. The optical system forms an optical image of the object T on the light receiving surface of the imaging element. The imaging element converts the optical image into image data and outputs it to the control device 70.

[0039] 3 is a diagram showing the configuration of the suction device 60. The suction device 60 sucks the contents of the target object T. The suction device 60 includes a suction pump 61, a first pipe H1, a tank 62, a first valve 63, a pressure pump 64, a second pipe H2, and a second valve 65.

[0040] The suction pump 61 creates a negative pressure inside the capillary 20. The suction pump 61 is a vacuum pump. However, it goes without saying that the suction pump 61 is not limited to a vacuum pump.

[0041] The first pipe H1 connects the suction pump 61 and the capillary 20. The first pipe H1 is connected to the base end B of the capillary 20. The tank 62 is disposed in the first pipe H1. The tank 62 is hollow. The first valve 63 is disposed in the first pipe H1 between the tank 62 and the capillary 20, and is an electromagnetic valve that opens and closes the first pipe H1.

[0042] The pressure pump 64 applies pressure to the inside of the capillary 20. The second pipe H2 connects the pressure pump 64 and the capillary 20. Specifically, the second pipe H2 is connected to a first branch B1 located in the first pipe H1 between the first valve 63 and the capillary 20. That is, the pressure pump 64 is connected to the capillary 20 via the second pipe H2 and the first pipe H1. The second valve 65 is disposed in the second pipe H2 and is an electromagnetic valve that opens and closes the second pipe H2.

[0043] The control device 70 shown in FIG. 1 controls the extraction device 1. The control device 70 is a computer, and includes, for example, a CPU (Central Processing Unit), a ROM (Read Only Memory), a RAM (Random Access Memory), an internal storage unit, an input interface, and an output interface. The CPU, ROM, RAM, and internal storage unit are connected via an internal bus. The ROM stores programs such as BIOS. The internal storage unit is, for example, a hard disk drive (HDD) or flash memory, and stores operating system programs and application programs. The CPU uses the RAM as a work area and executes programs stored in the ROM or internal storage unit to realize various functions.

[0044] The control device 70 derives the position of the tip E of the capillary 20 relative to the object T based on the imaging data of the first imaging unit 51 and the image data of the second imaging unit 52, which are imaged from different directions. The control device 70 controls the moving device 40 to move the capillary 20 based on the position of the tip E of the capillary 20 relative to the object T.

[0045] Next, the case where the extractor 1 sucks the contents of the target object T will be described.

[0046] As a preparation before aspirating the contents C of the target object T, the control device 70 controls the moving device 40 to move the capillary 20 and position the tip E of the capillary 20 inside the target object T (inside the contents C). In other words, the outer periphery of the target object T is pierced by the tip E of the capillary 20, and the tip E of the capillary 20 is inserted into the target object T. The position of the tip E of the capillary 20 can be confirmed by image data from the imaging device 50. The suction device 60 aspirates the contents C in a state in which the tip E of the capillary 20 is positioned inside the target object T.

[0047] 4 is a diagram showing the state of the target object T when the contents C are aspirated. The target object T shown in FIG. 4 is in a container Y that stores a culture solution L. When the contents C of the target object T are aspirated, the tip E of the capillary 20 is located inside the outer periphery of the target object T and inside the contents C.

[0048] The content C of the object T is located inside the outer periphery of the object T, and is a portion of the object T into which the culture solution L has penetrated in a relatively small amount. In other words, the content C of the object T is a portion that is relatively little directly affected by the culture solution L.

[0049] 5 is a flowchart executed by the control device 70 when the extraction device 1 aspirates the contents C. At the start of the flowchart shown in FIG. 5, the first valve 63 and the second valve 65 are both closed. In addition, when the tip E of the capillary 20 is inserted into the object T shown in FIG. 4, the culture solution L is present inside the capillary 20 due to capillary action.

[0050] 5, the control device 70 sets the pressure inside the tank 62 to negative pressure. Specifically, the control device 70 drives the suction pump 61 at a first drive amount for a first predetermined time while keeping the first valve 63 closed. The first drive amount and the first predetermined time are determined so that the pressure inside the tank 62 becomes negative and, when this pressure acts on the inside of the capillary 20, the content C can be sucked.

[0051] Next, in step S2, the control device 70 pressurizes the portion of the second pipe H2 between the pressure pump 64 and the second valve 65. Specifically, the control device 70 drives the pressure pump 64 at a second drive amount for a second predetermined time while keeping the second valve 65 closed. The second drive amount and the second predetermined time are determined so that the pressure in the portion of the second pipe H2 becomes higher than atmospheric pressure and does not cause the target T to come off the tip E of the capillary 20 when this pressure acts on the inside of the capillary 20.

[0052] Furthermore, in step S3, the control device 70 pressurizes the inside of the capillary 20. Specifically, the control device 70 closes the first valve 63 and opens the second valve 65, and drives the pressure pump 64 at the second drive amount for a third predetermined time. The second drive amount and the third predetermined time are determined to be a pressure at which the liquid level of the culture solution L inside the capillary 20 drops without the target T being removed from the tip E of the capillary 20. That is, in step S3, the culture solution L inside the capillary 20 is discharged.

[0053] Next, in step S4, the control device 70 maintains the pressure inside the capillary 20. Specifically, the control device 70 closes the first valve 63 and the second valve 65 until a fourth predetermined time has elapsed since the second valve 65 was closed. The fourth predetermined time is the time it takes for the state inside the capillary 20 to stabilize, and is, for example, 30 seconds.

[0054] Furthermore, in step S5, the control device 70 aspirates the contents C of the target object T. Specifically, the control device 70 opens the first valve 63 and closes the second valve 65, and drives the suction pump 61 at the first drive amount for a fifth predetermined time. The first drive amount and the fifth predetermined time are determined so that the pressure inside the capillary 20 becomes negative and is high enough to aspirate the contents C. That is, in step S5, the contents C are aspirated. The first drive amount and the fifth predetermined time are also determined so that not all of the contents C are aspirated, but rather a portion of the contents C and the outer periphery of the target object T are aspirated.

[0055] In this way, the control device 70 closes the first valve 63 in step S1 so that the suction pump 61 creates a negative pressure in the tank 62, and then opens the first valve 63 in step S5 so that the suction pump 61 creates a negative pressure inside the capillary 20. In this case, the time it takes for the pressure in the first pipe H1 to become negative is shorter than when the suction device 60 does not include the tank 62 and suction by the suction pump 61 is started from a state in which the pressure in the first pipe H1 is close to atmospheric pressure. In other words, when the first valve 63 is switched from the closed state to the open state, the negative pressure in the tank 62 acts on the inside of the capillary 20 first, and then the negative pressure generated by suction by the suction pump 61 acts on the inside of the capillary 20.

[0056] In step S2, the suction device 60 closes the first valve 63 and opens the second valve 65 to pressurize the inside of the capillary 20 with the pressure pump 64, and then in step S5 opens the first valve 63 and closes the second valve 65 to create a negative pressure inside the capillary 20 with the suction pump 61. As a result, the suction device 60 discharges the culture solution L inside the capillary 20 and then aspirates the contents C.

[0057] Subsequently, in step S6, the control device 70 checks whether the contents C have been extracted. Specifically, the control device 70 closes the first valve 63 and the second valve 65 and checks, based on the image data of the second imaging unit 52, whether the height H of the contents C inside the capillary 20 shown in Fig. 4 is equal to or greater than a predetermined height. The predetermined height is set to a height corresponding to the amount of contents C that can be analyzed.

[0058] If the height H of the contents C is lower than the predetermined height (NO in step S6), the control device 70 returns the program to step S3 and performs suction of the contents C again.

[0059] On the other hand, if the height H of the contents C is equal to or greater than the predetermined height (YES in step S6), the control device 70 ends the program.

[0060] After aspirating the contents C, the control device 70 moves the capillary 20 using the moving device 40, and discharges the contents C from the capillary 20 using the pressure pump 64. The discharged contents C are mainly used as an analysis sample.

[0061] As described above, according to this embodiment, the extraction device 1 comprises a tubular capillary 20 and a suction device 60 that creates negative pressure inside the capillary 20 and sucks the contents C of the target object T from the tip E of the capillary 20.

[0062] According to this, the contents C of the target object T are sucked by the suction device 60 and held inside the capillary 20. Therefore, the extraction device 1 can extract the contents C of the target object T appropriately.

[0063] The suction device 60 further includes a suction pump 61, a first pipe H1 connecting the suction pump 61 and the capillary 20, a hollow tank 62 arranged in the first pipe H1, and a first valve 63 arranged in the first pipe H1 between the tank 62 and the capillary 20 and opening and closing the first pipe H1. The suction device 60 closes the first valve 63 to make the pressure inside the tank 62 negative by the suction pump 61, and then opens the first valve 63 to make the pressure inside the capillary 20 negative by the suction pump 61.

[0064] According to this, when suction of the content C starts, as described above, the negative pressure in the tank 62 first acts on the inside of the capillary 20 early on. Therefore, compared to when the suction device 60 does not include the tank 62, the suction force acting on the target object T can be stabilized.

[0065] The suction device 60 also includes a pressure pump 64, a second pipe H2 connecting the pressure pump 64 and the capillary 20, and a second valve 65 disposed in the second pipe H2 and opening and closing the second pipe H2. The suction device 60 pressurizes the inside of the capillary 20 with the pressure pump 64 by closing the first valve 63 and opening the second valve 65, and then creates a negative pressure inside the capillary 20 with the suction pump 61 by opening the first valve 63 and closing the second valve 65.

[0066] According to this, when the object T is in a fluid such as a culture solution L, it is possible to discharge the fluid or the like that has flowed into the inside of the capillary 20 before aspirating the contents C of the object T. Therefore, the extraction device 1 can prevent extraction of anything other than the contents C of the object T.

[0067] The suction pump 61 is a vacuum pump.

[0068] This allows the suction pump 61 to reliably suck the contents C of the target object T.

[0069] Furthermore, the object T is a cell mass.

[0070] This allows the extraction device 1 to appropriately extract the contents of the cell clusters. Therefore, the extraction device 1 can provide the contents of the cell clusters that are relatively less subject to external direct influences (for example, direct influences from the culture solution L) as an analysis sample.

[0071] The extraction device 1 further includes a moving device 40 that moves the capillary 20. The moving device 40 moves the capillary 20 so that the tip E of the capillary 20 is positioned inside the target object T. The suction device 60 suctions the contents C in a state in which the tip E of the capillary 20 is positioned inside the target object T.

[0072] This allows the extraction device 1 to reliably suck up the contents C of the target object T.

[0073] The outer diameter of the tip portion 22 of the capillary 20 decreases from the base end B side of the capillary 20 toward the tip E side.

[0074] According to this, the tip E of the capillary 20 pierces the outer shell of the target T, so that the tip E of the capillary 20 is reliably positioned inside the target T. Therefore, the extraction device 1 can reliably aspirate the contents C of the target T.

[0075] The extraction method also includes the steps of moving the tubular capillary 20 to position the tip E of the capillary 20 inside the object T, and creating negative pressure inside the capillary 20 with the suction device 60 while the tip E of the capillary 20 is positioned inside the object T, and sucking the contents C of the object T from the tip E of the capillary 20.

[0076] According to this, the contents C of the target object T are sucked by the suction device 60 and held inside the capillary 20. Therefore, the contents C of the target object T can be appropriately extracted.

[0077] Next, an extraction device 1 according to a modified example of the embodiment of the present disclosure will be described.

[0078] For example, the extraction device 1 may extract the contents C in a state where the object T is not present in the culture solution L.

[0079] The suction device 60 may also aspirate the contents C in a state where the tip E of the capillary 20 is not positioned inside the target object T. In this case, the suction device 60 aspirates the contents C in a state where the tip E of the capillary 20 is in contact with the outer surface of the target object T. The contents C are aspirated into the inside of the capillary 20 as the outer shell of the target object T is torn.

[0080] In addition, in the flowchart shown in FIG. 5, the control device 70 may execute steps S2, S3, and S4 before step S1, and then execute steps S1 and S5 after executing step S4.

[0081] Furthermore, the suction device 60 does not have to include the tank 62. In this case, the control device 70 does not execute step S1 shown in FIG.

[0082] Furthermore, the suction device 60 does not necessarily have to include the pressure pump 64, the second pipe H2, and the second valve 65. In this case, the control device 70 does not execute steps S2, S3, and S4 shown in FIG.

[0083] 1, the extraction device 1 may further include a hardness deriving device 180. The hardness deriving device 180 is a part of the control device 70. Note that the hardness deriving device 180 may be separate from the control device 70.

[0084] The hardness deriving device 180 derives the hardness of the object T based on the amount of the contents C sucked into the capillary 20 by the suction device 60. Specifically, after the flowchart shown in FIG. 5 is completed, the hardness deriving device 180 derives the hardness of the object T based on the height H of the contents C inside the capillary 20 shown in FIG. 4. The height H of the contents C is proportional to the amount of the contents C. The correlation between the height H of the contents C and the hardness of the object T is stored in a memory area of the hardness deriving device 180 for each type of object T.

[0085] According to this modification, the extraction device 1 further includes a hardness deriving device 180 that derives the hardness of the target object T based on the amount of the content C sucked into the inside of the capillary 20 by the suction device 60.

[0086] This allows the extraction device 1 to derive the hardness of the object T when the contents C of the object T are aspirated.

[0087] 6 is a diagram showing the configuration of a suction device 60 according to a modified example of the embodiment of the present disclosure. In this modified example, the suction device 60 further includes a third pipe H3 and a third valve 266.

[0088] The third pipe H3 has a first end connected to the second branch B2 of the first pipe H1 between the suction pump 61 and the tank 62, and a second end open to the atmosphere. The third valve 266 is disposed in the third pipe H3 and is an electromagnetic valve that opens and closes the third pipe H3.

[0089] 5, the control device 70 closes the third valve 266 in steps S1, S2, S3, S4, and S6. In step S5, the control device 70 opens the first valve 63 and the third valve 266 and closes the second valve 65 to aspirate the contents C. In this case, the suction pump 61 aspirates the contents C into the inside of the capillary 20 and also aspirates the atmosphere via the third pipe H3.

[0090] According to this modification, the suction device 60 further includes a third pipe H3, the first end of which is connected to the first pipe H1 between the suction pump 61 and the tank 62 and the second end of which is open to the atmosphere, and a third valve 266 disposed in the third pipe H3 and configured to open and close the third pipe H3. The suction device 60 opens the first valve 63 and the third valve 266 to create a negative pressure inside the capillary 20 using the suction pump 61.

[0091] According to this, as described above, the suction pump 61 sucks in the atmosphere via the third pipe H3 when sucking the contents C of the target object T. This reduces the suction force acting on the contents C of the target object T, and prevents the entire target object T, including the contents C and outer shell, from being sucked into the capillary 20 prematurely. Therefore, the extraction device 1 can accurately suck in the contents C of the target object T.

[0092] The suction device 60 may also include a first pressure sensor and a second pressure sensor. The first pressure sensor is disposed in the first pipe H1 between the tank 62 and the first valve 63. The second pressure sensor is disposed in the second pipe H2 between the second valve 65 and the pressure pump 64. The detection results of the first pressure sensor and the second pressure sensor are output to the control device 70. In this case, the control device 70 may control the drive amount of the suction pump 61 in steps S1 and S5 so that the pressure detected by the first pressure sensor becomes a pressure at which the content C can be sucked. The control device 70 may also control the drive amount of the pressure pump 64 in steps S2 and S3 so that the pressure detected by the second pressure sensor becomes a pressure at which the culture solution L is discharged from the inside of the capillary 20. [Explanation of symbols]

[0093] 1 Extraction device 20 Capillary 40 Mobile Device 60 Suction device 61 Suction pump 62 Tank 63 First valve 64 Pressure pump 65 Second valve 180 Hardness derivation device 266 Third Valve C. Contents of the object E Capillary tip H1 First piping H2 Second piping H3 3rd piping T object

Claims

1. A tubular capillary; a suction device that creates a negative pressure inside the capillary and sucks the contents of the object from the tip of the capillary; Extraction device.

2. The suction device is A suction pump; a first pipe connecting the suction pump and the capillary; a hollow tank disposed in the first pipe; a first valve disposed in the first pipe between the tank and the capillary and configured to open and close the first pipe; after the first valve is closed and the pressure in the tank is made negative by the suction pump, the first valve is opened and the pressure inside the capillary is made negative by the suction pump; The extraction device of claim 1 .

3. The suction device is A pressure pump and a second pipe connecting the pressure pump and the capillary; a second valve disposed on the second pipe and configured to open and close the second pipe; After the first valve is closed and the second valve is opened to pressurize the inside of the capillary by the pressure pump, the first valve is opened and the second valve is closed, and a negative pressure is created inside the capillary by the suction pump; The extraction device of claim 2.

4. The suction device is a third pipe having a first end connected to the first pipe between the suction pump and the tank and a second end open to the atmosphere; a third valve disposed on the third pipe and configured to open and close the third pipe; the first valve and the third valve are opened, and a negative pressure is created inside the capillary by the suction pump; The extraction device of claim 2.

5. The suction pump is a vacuum pump. The extraction device of claim 2.

6. The object is a cell mass. The extraction device of claim 1 .

7. Further comprising a moving device for moving the capillary, the moving device moves the capillary so that the tip of the capillary is positioned inside the object; the suction device aspirates the contents while the tip of the capillary is positioned inside the object; The extraction device of claim 1 .

8. The apparatus further includes a hardness calculation device that calculates the hardness of the object based on the amount of the content sucked into the capillary by the suction device. The extraction device of claim 1 .

9. The tip portion of the capillary has an outer diameter that decreases from the base end side to the tip end side of the capillary. The extraction device of claim 1.

10. A step of moving a tubular capillary to position a tip of the capillary inside the object; a step of creating a negative pressure inside the capillary with a suction device while the tip of the capillary is positioned inside the object, and aspirating the contents of the object from the tip of the capillary; Extraction method.

Images