Reaction vessel and synthesis apparatus

Abstract

To enable uniformization of a carrier flow within a reaction vessel between a center side and a sidewall side of a reaction chamber.SOLUTION: A reaction vessel 10 includes: a container body 15 having a reaction chamber 16 that stores a carrier B and holds a solution L; a supply port 17 provided at a lower part 151 of the container body 15 for introducing a gas G; a discharge port 18 provided at an upper part 152 of the container body 15 for releasing the gas G; and a stirring blade 30 provided in the reaction chamber 16, floating in the solution L, and rotated by the gas G introduced through the supply port 17.SELECTED DRAWING: Figure 2

Description

[Technical Field]

[0001] The presently disclosed invention relates to a synthesis apparatus for chemically synthesizing proteins, peptides, nucleic acids, etc., and a reaction vessel included in the synthesis apparatus. [Background technology]

[0002] One method for chemically synthesizing proteins, peptides, nucleic acids, etc. involves sequentially supplying multiple types of solutions (reagents) to a reaction vessel and allowing the reaction to proceed in the reaction vessel. For example, when synthesizing nucleic acids, a large number of granular carriers (beads) are placed in the reaction vessel, and while sequentially supplying solutions to the reaction vessel, detritylation, coupling, oxidation, and capping processes are repeatedly carried out to bind bases to the carriers.

[0003] There are sometimes several tens of types of solutions used in the above-described chemical synthesis. These solutions are selectively sent to a reaction vessel, and the target product (nucleic acid) is produced from the molecular materials contained in the solution. For example, a synthesis apparatus disclosed in Patent Document 1 is known as an apparatus for performing such chemical synthesis. The synthesis apparatus has a reaction vessel. The reaction vessel has a reaction chamber in which a solution is stored and a reaction using the solution is carried out. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 2019-34290 Summary of the Invention [Problem to be solved by the invention]

[0005] Figure 7 is an explanatory diagram of a conventional reaction vessel. Figure 8 is an explanatory diagram showing the state of chemical synthesis processing using a conventional reaction vessel. Reaction vessel 90 is located in a part of circulation piping 94 having a pump 95. A large number of carriers B are present in reaction vessel 90. A solution is supplied from the bottom of reaction vessel 90 by pump 95. The solution fills reaction chamber 91 of reaction vessel 90, is discharged from the top, and circulates through circulation piping 94.

[0006] In recent years, in the chemical synthesis process described above, long chain synthesis has been carried out in which the bases extending from carrier B become longer. The more bases are built up from carrier B, the lower the synthesis purity becomes. One of the reasons for this is that the flow rate of the solution (fluid) flowing through the reaction chamber 91 differs between the central side 911 (inside) and the side wall side 912 (outside), and the solution L and the carrier B are unlikely to be exchanged between the central side 911 and the side wall side 912. As a result, the finish of the chemical synthesis reaction accumulated from the carrier B differs between the central side 911 and the side wall side 912, resulting in a decrease in synthesis purity.

[0007] Therefore, an object of the present invention is to provide a reaction vessel that can make the flow of carriers uniform in a reaction chamber, and a synthesis apparatus having such a reaction vessel. [Means for solving the problem]

[0008] (1) The reaction vessel of the present invention comprises a vessel body having a reaction chamber for accommodating a carrier and storing a solution, a supply port provided at the bottom of the vessel body for supplying gas, an outlet provided at the top of the vessel body for discharging gas, and an agitator blade provided in the reaction chamber, which is suspended in the solution and rotates due to the gas supplied from the supply port.

[0009] In a reaction vessel having the above configuration, when gas is supplied from the supply port to the reaction chamber of the vessel body, the gas rises through the solution stored in the reaction chamber and is discharged from the discharge port. The stirring blades are rotated by the gas rising through the solution. The rotation of the stirring blades creates a circulating flow of the solution in the reaction chamber, and this circulating flow makes it possible to uniformize the flow of the carrier. In order to rotate the stirring blades, it is only necessary to supply gas to the reaction chamber, and no driving device such as a motor is required.

[0010] (2) Preferably, the stirring blade has a central portion, a plurality of blade portions extending from the central portion toward the side wall of the reaction chamber, and a linear portion extending from the central portion toward the upper or lower surface of the reaction chamber, and the linear portion has a contact portion that can contact the upper or lower surface of the reaction chamber. According to the above-mentioned configuration, the stirring blade is supported by the straight portion on the upper or lower surface of the reaction chamber, allowing the blade portion to rotate stably.

[0011] (3) In the reaction vessel of (2) above, the contact portion preferably has a spherical shape or a tapered shape. According to the above-mentioned configuration, the frictional resistance between the contact portion of the straight portion and the upper or lower surface of the reaction chamber is reduced, and the stirring blade rotates with a small force.

[0012] (4) In the reaction vessel of (2) or (3), preferably, the upper or lower surface of the reaction chamber has a contacted surface with which the contact portion comes into contact, and the contacted surface has a recess into which the contact portion is inserted. According to this configuration, the contact portion of the straight portion fits into the recess in the upper or lower surface of the reaction chamber, and the stirring blade is stably guided to rotate.

[0013] (5) In the reaction vessel of any one of (1) to (4) above, the stirring blade preferably has a hollow portion. According to the above configuration, the buoyancy of the stirring blade can be increased and the buoyancy can be easily set, so that the stirring blade can be set at an appropriate height in the solution.

[0014] (6) In any one of the reaction vessels (1) to (5), the stirring blade preferably has a central portion and three or more blade portions extending from the central portion toward the side wall of the vessel body and arranged at equal intervals around the central portion. According to this configuration, the stirring blade is stabilized in a floating state in the solution.

[0015] (7) The synthesis apparatus of the present invention comprises a liquid delivery means for selectively delivering multiple types of solutions, and a reaction vessel of any one of (1) to (6) for storing the solutions delivered by the liquid delivery means and carrying out a reaction using the solutions, wherein the liquid delivery means has a pipe connected to the supply port, and the pipe has an adjustment unit for adjusting the flow rate of the gas.

[0016] The synthesis apparatus having the above configuration includes any one of the reaction vessels (1) to (6). Therefore, when gas is supplied to the reaction chamber from the supply port, the gas rotates the stirring blade. This rotation generates a circulating flow of the solution in the reaction chamber, and this circulating flow makes it possible to uniformize the flow of the carrier.

[0017] (8) In the synthesis apparatus of (7), a gas having a pressure higher than atmospheric pressure is supplied to the reaction vessel from the supply port. With this configuration, the agitating blades can be effectively rotated by the gas supplied from the supply port. [Effects of the Invention]

[0018] According to the present invention, the circulating flow makes it possible to make the flow of the carrier uniform, which results in an improvement in the purity of, for example, a chemical synthesis reaction. [Brief explanation of the drawings]

[0019] [Figure 1] 1 is a configuration diagram illustrating an example of a synthesis device of the present invention. [Figure 2] FIG. 2 is an explanatory diagram of a reaction vessel. [Figure 3]FIG. 2 is an explanatory diagram showing the state of stirring by a stirring blade. [Figure 4] FIG. 10 is an explanatory diagram showing a modified example of the stirring blade. [Figure 5] FIG. 2 is an explanatory diagram of the upper portion of the straight portion and the upper surface of the reaction chamber. [Figure 6] FIG. 10 is an explanatory diagram showing yet another modified example of the stirring blade. [Figure 7] FIG. 1 is an explanatory diagram of a conventional reaction vessel. [Figure 8] FIG. 1 is an explanatory diagram showing a chemical synthesis process using a conventional reaction vessel. DETAILED DESCRIPTION OF THE INVENTION

[0020] [About the synthesis equipment] FIG. 1 is a block diagram showing an example of a synthesis apparatus of the present invention. The synthesis apparatus 70 shown in FIG. 1 is an apparatus for chemically synthesizing proteins, peptides, nucleic acids, etc. The synthesis apparatus 70 includes a reaction vessel 10. The reaction vessel 10 is also called a reactor. Multiple types of solutions (reagents) L are sequentially supplied to the reaction vessel 10, and chemical synthesis proceeds within the reaction vessel 10. To synthesize nucleic acids, a large number of granular carriers B are provided within the reaction vessel 10. The carriers B are also called beads, and are made of, for example, glass or polymer. In each drawing, the carriers B are shown larger than they actually are to make the explanation easier to understand.

[0021] While the solution L is sequentially supplied to the reaction vessel 10, the processes of detritylation, coupling, oxidation, and capping are repeatedly carried out, and bases (molecular materials) are successively bound from the carrier B. There are cases where several tens of types of solution L are used for chemical synthesis. These solutions L are selectively (alternatively) sent to the reaction vessel 10, and the target product (nucleic acid) is produced from the molecular materials contained in the solution L.

[0022] The synthesis apparatus 70 has an area for providing the same number of storage containers (reagent bottles) 71 as the number of solutions L to be used. Each storage container 71 stores a respective solution L. Note that only three storage containers 71 are shown in FIG. 1, and the other storage containers 71 are not shown. Each storage container 71 is a sealed container, and is connected to an inlet pipe 72 and an outlet pipe 73.

[0023] The synthesis apparatus 70 includes a tank 78 storing gas G, an upstream pipe 79, an inlet pipe 72, an outlet pipe 73, a measuring vessel 74, an intermediate pipe 75, a reaction vessel 10, a measuring mechanism 76, and a control device 77. The control device 77 is configured by a computer. The tank 78 is filled with gas G (e.g., an inert gas) at a pressure higher than the atmosphere. This gas G is used to transport the solution L from the storage vessel 71 to the reaction vessel 10, and is also used to stir the carrier B and the solution L in the reaction vessel 10 with the stirring blades 30.

[0024] The same number of introduction pipes 72 as the number of storage containers 71 are pipes branching off from a common upstream pipe 79. A regulator 80 and a valve 81 are provided on the upstream pipe 79. The upstream pipe 79 is connected to a tank 78, and gas G from the tank 78 is supplied to each storage container 71, and the internal pressure of each storage container 71 is adjusted by the regulator 80. The internal pressure of each storage container 71 is increased by the gas G, and the solution L in the storage container 71 is sent out from the outlet pipe 73. The differential pressure between each storage container 71 and the measuring container 74 causes the solution L in each storage container 71 to be pressure-fed through the outlet pipe 73 to the measuring container 74.

[0025] Each outlet pipe 73 is provided with a valve 82. By selecting a valve 82 to be opened, a predetermined solution L is selectively sent from the solutions L in the multiple storage containers 71 to the measuring container 74 through the outlet pipe 73. The valve 82 to be opened is selected by the control device 77.

[0026] The measuring container 74 is a container for measuring each solution L. A plurality of outlet pipes 73 are provided in an inlet region (opening) of the measuring container 74. The solution L sent through the outlet pipes 73 is introduced into the measuring container 74 and stored in the measuring container 74.

[0027] The weighing mechanism 76 has a measuring device such as a load cell, and measures the solution L stored in the weighing container 74. The weighing result by the weighing mechanism 76 is sent to the control device 77. The control device 77 controls the opening and closing operation of the valve 82 based on the weighing result, and obtains a specified amount of solution L in the weighing container 74. The specified amount of solution L is sent to the reaction container 10 through the intermediate piping 75.

[0028] The method for supplying solution L from measuring vessel 74 to reaction vessel 10 is pressure-feeding with gas G, and gas G from tank 78 is used. For this pressure-feeding, synthesis apparatus 70 is provided with a sealed vessel 83 that houses measuring vessel 74. A pipe 84 for gas G is provided between sealed vessel 83 and tank 78. A second regulator 85 and a valve 86 are provided in pipe 84. Measuring container 74 is open inside sealed container 83, and when gas G from tank 78 is supplied to sealed container 83, the pressure (internal pressure) of gas G inside sealed container 83 acts on solution L stored in measuring container 74. Valve 89 is provided in intermediate piping 75, and when valve 89 changes from a closed state to an open state, the solution L in measuring container 74 is pressure-fed to reaction container 10 through intermediate piping 75 due to the pressure difference between sealed container 83 (measuring container 74) and reaction container 10.

[0029] As described above, the solution L is selectively sent from the plurality of storage containers 71 to the measuring container 74, and after being measured in the measuring container 74, the solution L is sent to the reaction container 10. The solution L is used for chemical synthesis in the reaction container 10. Thereafter, the solution L is discharged to the waste liquid tank 88 through the discharge pipe 87. Such supply of the solution L to the reaction vessel 10 is repeated while changing the type of solution L. A plurality of types of solutions L are sequentially supplied to the reaction vessel 10, and chemical synthesis proceeds within the reaction vessel 10.

[0030] As described above, the synthesis apparatus 70 has a liquid delivery means 20 for selectively (alternatively) transporting multiple types of solutions L. In this embodiment, the liquid delivery means 20 uses the pressure of the gas G to pressure-feed the solution L from the storage container 71 downstream toward the reaction container 10. To this end, the liquid delivery means 20 is configured to include a tank 78 that stores the gas G, an upstream pipe 79 connected to the tank 78, a plurality of inlet pipes 72 that branch off from the upstream pipe 79 and are connected to each storage container 71, an outlet pipe 73 that leads the solution L from each storage container 71, a regulator 80 for adjusting the flow rate, a valve 81 for the gas G, and a liquid delivery valve 82.

[0031] The reaction vessel 10 can store the solution L transported by the liquid transport means 20, and reactions using various solutions L are carried out in the reaction vessel 10. In the present embodiment (see FIG. 2), the solution L is stored up to a position lower than the upper surface of the reaction chamber 16. FIG. 2 is an explanatory diagram of the reaction vessel 10.

[0032] [Regarding the reaction vessel 10] The reaction vessel 10 has a vessel body 15. The vessel body 15 has a cylindrical tubular member 11 and flange portions 12 connected to the upper and lower portions of the tubular member 11. The tubular member 11 is a portion where a solution L is stored and a reaction takes place inside. A filter 13 is provided on each of the upper and lower flange portions 12. The filter 13 allows the solution L to pass through but does not allow carriers (beads) B to pass through. The space inside the tubular member 11, between the upper and lower filters 13, forms a reaction chamber 16 where a reaction with the solution L takes place. The reaction chamber 16 of the vessel body 15 contains a large number of carriers B. The solution L is stored in the reaction chamber 16.

[0033] The reaction vessel 10 has a supply port 17 provided in a lower portion 151 of the vessel body 15 and a discharge port 18 provided in an upper portion 152 of the vessel body 15. A pipe through which gas G flows is connected to the supply port 17. In the present embodiment (see FIG. 1), a pipe 84 for gas G connected to the tank 78 has a branch portion 841. The branch portion 841 branches into two, one pipe 842 connected to the sealed vessel 83, and the other pipe 843 connected to a switching valve (three-way valve) 844 located midway along the intermediate pipe 75.

[0034] The switching valve 844 can be switched alternatively between a first state and a second state by changing the position of its valve element. In the first state, the solution L in the measuring container 74 can be supplied to the reaction container 10, but the supply of gas G to the reaction container 10 through the piping 843 is disabled. In the second state, the gas G can be supplied to the reaction container 10 through the piping 843, but the supply of the solution L in the measuring container 74 to the reaction container 10 is disabled. The operation of the switching valve 844 is controlled by the control device 77.

[0035] When the switching valve 844 is in the second state, gas G is supplied to the supply port 17 of the reaction vessel 10. In this embodiment (see FIG. 2), the reaction vessel 10 has a filter 13 in the lower part 151. The supply port 17 is located below the filter 13. A small space 91 is provided between the supply port 17 and the filter 13. The gas G that has passed through the supply port 17 enters the small space 91, passes through the filter 13, and is supplied to the reaction chamber 16. The gas G is supplied to the reaction chamber 16 from the entire lower part (the entire surface of the filter 13).

[0036] When gas G is supplied to reaction chamber 16 through supply port 17, the gas G rises in solution L stored in reaction chamber 16 and is discharged through discharge port . In this embodiment, the reaction vessel 10 has a filter 13 in the upper portion 152. The exhaust port 18 is located above the filter 13. A small space 92 is provided between the filter 13 and the exhaust port 18. The gas G in the reaction chamber 16 passes through the filter 13, enters the small space 92, and is exhausted through the exhaust port 18. In this way, the gas G inside the reaction chamber 16 is discharged through the exhaust port 18. In the present embodiment, the exhaust port 18 is connected to an exhaust pipe 87 and is open to the atmosphere.

[0037] [Regarding the mixing blade 30] The reaction vessel 10 has an agitator blade 30. The agitator blade 30 is provided in the reaction chamber 16 and is suspended in the solution L as shown in FIG. 2. The "suspended state" will be explained later. When the solution L is not present in the reaction chamber 16, the agitator blade 30 rests on the bottom surface (lower filter 13) of the reaction chamber 16. FIG. 3 is an explanatory diagram showing the state of stirring of the solution L and carrier B by the agitator blade 30. FIG. 3 shows the agitator blade 30 in a suspended state. The agitator blade 30 rotates due to gas G supplied from the supply port 17.

[0038] The agitator blade 30 is made of, for example, resin. The agitator blade 30 has a hollow portion 35. In the form shown in FIG. 3, the agitator blade 30 has a central portion 31 and three blade portions 32. The central portion 31 is the central portion of the agitator blade 30. The center of the central portion 31 is located on the rotation center line (design rotation center line) C1 of the agitator blade 30. Each blade portion 32 has a hollow portion 35. Note that the central portion 31 may also have a hollow portion.

[0039] The blade portions 32 extend from the central portion 31 toward the side wall side 162 of the container body 15. The three blade portions 32 are arranged at equal intervals around the central portion 31. It is preferable that there are three or more blade portions 32. When each blade portion 32 receives a fluid flowing from below to above, it generates a rotational force in the mixing impeller 30 centered on the central portion 31. The rotational force is a force centered on the vertical rotation center line C1.

[0040] The stirring blade 30 has three or more blade portions 32 arranged at equal intervals around the central portion 31, and is therefore stable in a floating state in the solution L. The agitator blade 30 has a hollow portion 35, which increases the buoyancy of the agitator blade 30 and makes it easier to set (design) the buoyancy. This allows the agitator blade 30 to be set at an appropriate height in the solution L. The agitator blade 30 can remain at a predetermined position in the solution L in the vertical direction.

[0041] The agitator 30 is configured so that when the rotation center line (design rotation center line) C1 of the agitator 30 is oriented vertically, the center of gravity of the agitator 30 and the center of buoyancy (center of buoyancy) of the agitator 30 are located on the rotation center line C1. The rotation center line C1 of the agitator 30 (rotation axis of the agitator 30) is maintained along the vertical direction. This stabilizes the position of the agitator 30 floating in the solution L.

[0042] The function of the stirring blade 30 will be described. As described above, when gas G is supplied from supply port 17 to reaction chamber 16 of container body 15, the gas G rises in solution L stored in reaction chamber 16 and is discharged from discharge port . The blade portion 32 of the agitating impeller 30 receives an upward force from the rising gas G and solution L. This force, that is, the gas G rising in the solution L, causes the agitating impeller 30 to rotate.

[0043] The rotation of the stirring blade 30 causes a circulating flow of the solution L in the reaction chamber 16. This circulating flow makes the flow of the carrier B uniform. In Figure 3, the circulating flow of the solution L is indicated by arrow F. The circulating flow is a flow in which the fluid L flows toward the upper part 152 at the central side 161, changes direction, flows toward the side wall side 162, further proceeds toward the lower part 151, changes direction, and returns to the central side 161. The circulating flow generated by the stirring blades 30 allows the carrier B to be regularly exchanged between the center side 161 and the side wall side 162 of the reaction chamber 16, and the flow of the carrier B is made uniform.

[0044] That is, although a large number of carriers B are present in the reaction chamber 16, all of the carriers B circulate along the circulation flow between the center side 161 and the side wall side 162 of the reaction vessel 10, where the flow speed of the solution L is different. This makes it possible to carry out a chemical synthesis reaction under the same conditions in all of the carriers B, and the end result of the chemical synthesis reaction in each carrier B is uniform. Differences in the end result of the chemical synthesis reaction accumulated from the carriers B are unlikely to occur between the center side 161 and the side wall side 162 of the reaction chamber 16, preventing a decrease in synthesis purity.

[0045] In the present embodiment, in order to rotate the stirring blades 30, it is only necessary to supply the gas G to the reaction chamber 16, and a driving device such as a motor and a driving shaft are not required. In order to effectively rotate the stirring blades 30 by the gas G supplied from the supply port 17, the gas G having a pressure higher than atmospheric pressure (slightly pressurized gas G) is supplied from the supply port 17 to the reaction vessel 10.

[0046] In this embodiment (see FIG. 1), the liquid delivery means 20 has a pipe 90 that connects the tank 78 storing the gas G to the supply port 17. The pipe 90 has an adjustment unit that adjusts the flow rate of the gas G. In the embodiment shown in FIG. 1, the pipe 90 that connects the tank 78 to the supply port 17 is made up of a part of the pipe 84 for the gas G, a (branched) pipe 843, and a part of the intermediate pipe 75. The adjustment unit is a second regulator 85 of the pipe 84. Note that the adjustment unit may be separate from the second regulator 85.

[0047] The adjusting unit may be provided between the switching valve (three-way valve) 844 and the reaction vessel 10, or may be provided in the (branched) pipe 843. The adjusting unit adjusts the flow rate (pressure) of the gas G, and adjusts the force that rotates the stirring blade 30.

[0048] [Modification of the stirring blade 30] FIG. 4 shows a modified example of the agitator blade 30. Like the agitator blade 30 shown in FIG. 3, the agitator blade 30 shown in FIG. 4 has a central portion 31 and multiple blade portions 32 extending from the central portion 31 toward the side wall 162 of the reaction chamber 16. The central portion 31 and blade portions 32 are the same as those of the agitator blade 30 shown in FIG. 3. The blade portion 32 may have a hollow portion 35. The agitator blade 30 shown in FIG. 4 further has a straight portion 33. The straight portion 33 extends from the central portion 31 toward the upper surface 163 of the reaction chamber 16. The straight portion 33 has a contact portion 34 that can come into contact with the upper surface 163 of the reaction chamber 16.

[0049] 4, the stirring blade 30 is supported by the upper surface 163 of the reaction chamber 16 via the straight portion 33. This allows the blade portion 32 to rotate stably. The rotation of the blade portion 32 causes a circulating flow of the solution L in the reaction chamber 16, as described above.

[0050] FIG. 5 is an explanatory diagram of the upper portion of the straight portion 33 and the upper surface 163 of the reaction chamber 16. The contact portion 34 at the upper end of the straight portion 33 has a tapered shape. The contact portion 34 may also have a spherical shape. In the configuration shown in FIG. 5, the contact portion 34 has a tapered shape that narrows toward the top, and the tip of the tapered portion has a spherical shape. With such a configuration of the contact portion 34, frictional resistance between the contact portion 34 and the upper surface 163 of the reaction chamber 16 is reduced, and the stirring blade 30 can be rotated with a small force.

[0051] The upper surface 163 of the reaction chamber 16 has a contacted surface 39 with which the contact portion 34 comes into contact. In the present embodiment, the upper surface 163 of the reaction chamber 16 is the lower surface of the upper filter 13 (see FIG. 2). The area that becomes the contacted surface 39 does not necessarily have to function as a filter.

[0052] As shown in Fig. 5, the contacted surface 39 has a recess 391 into which the contact portion 34 is inserted. The shape of the recess 391 may be a spherical shape, or as shown in Fig. 5, it may be a conical shape with a diameter that decreases toward the top. The center line of the recess 391 coincides with the center line CL of the cylindrical reaction chamber 16 in the vertical direction. The recess 391 fits into the recess 391, and the agitator blade 30 is stably guided to rotate.

[0053] [Modification of the stirring blade 30] 6 is an explanatory diagram showing yet another modified example of the agitator blade 30. The agitator blade 30 may have a straight portion 33 extending from the central portion 31 toward the lower surface 164 of the reaction chamber 16. In this case, the straight portion 33 has a contact portion 34 that can come into contact with the lower surface 164 of the reaction chamber 16. The agitator blade 30 is supported by the lower surface 164 of the reaction chamber 16 via the straight portion 33. The contact portion 34 at the lower end of the straight portion 33 preferably has a tapered shape, or alternatively, the contact portion 34 may have a spherical shape. The lower surface 164 of the reaction chamber 16 has a contacted surface 39 that comes into contact with the contact portion 34. The contacted surface 39 has a recess 391 into which the contact portion 34 is inserted.

[0054] As described above (see FIGS. 4 and 6), the linear portion 33 extends from the central portion 31 toward the upper surface 163 or the lower surface 164 of the reaction chamber 16. The linear portion 33 has a contact portion 34 that can come into contact with the upper surface 163 or the lower surface 164 of the reaction chamber 16.

[0055] 4, for example, gas G supplied from supply port 17 to reaction chamber 16 exerts an upward thrust on stirring blade 30. When the sum of the thrust of gas G and the buoyancy of stirring blade 30 becomes greater than the gravity of stirring blade 30, stirring blade 30 tends to rise in solution L. In this case, as shown in FIG. 4, it is preferable that the stirring blade 30 has a straight portion 33 extending upward from a central portion 31.

[0056] On the other hand, when the sum of the thrust of the gas G and the buoyancy of the impeller 30 is smaller than the gravity of the impeller 30, the impeller 30 tends to descend in the solution L. In this case, as shown in FIG. 6, it is preferable that the stirring blade 30 has a straight portion 33 extending downward from a central portion 31.

[0057] Even when the agitator blade 30 has the straight portion 33, the agitator blade 30 may rotate in a floating state with the straight portion 33 not in contact with the upper surface 163 or the lower surface 164 of the reaction chamber 16. For this purpose, the buoyancy of the agitator blade 30 is adjusted, or the flow rate (pressure) of the gas G is adjusted during the synthesis process so that the sum of the thrust force of the gas G and the buoyancy of the agitator blade 30 is made equal to the gravity of the agitator blade 30.

[0058] As described above, the "floating state" of the agitator 30 in the present invention refers to a state in which the blade portion 32 of the agitator 30 is floating in the solution L, and includes a state in which, if the agitator 30 is subjected to an external force in one direction, vertically, the agitator 30 is movable in that direction. In other words, the agitator 30 in the solution L is in a floating state when it is not restrained in the vertical direction by the container body 15.

[0059] 4 and 6, the agitator blade 30 has a straight portion 33, and the contact portion 34 of the straight portion 33 can come into contact with the upper surface 163 or the lower surface 164 of the reaction chamber 16. For example, if the agitator blade 30 receives a downward external force while the contact portion 34 is in contact with the upper surface 163 of the reaction chamber 16, the agitator blade 30 can move downward, and it can be said that the agitator blade 30 is in a floating state.

[0060] When the impeller 30 has the straight portion 33, there is no need to adjust (strictly adjust) the sum of the thrust of the gas G and the buoyancy of the impeller 30 to match the gravity of the impeller 30. In other words, for example, it becomes easier to control the adjustment of the flow rate (pressure) of the gas G.

[0061] 〔others〕 The synthesis apparatus 70 may have a configuration other than that shown in Fig. 1. For example, the metering mechanism 76 may be configured to measure the storage container 71. In this case, the metering container 74 and the sealed container 83 may be omitted, and the multiple outlet pipes 73 may be joined upstream of the intermediate pipe 75. Furthermore, the liquid delivery means 20 may have a configuration other than that shown in the figure, as long as it has the function of selectively (alternatively) transporting multiple types of solutions L.

[0062] 1, a configuration has been described in which the supply of solution L to reaction vessel 10 and the supply of gas G for rotating agitator blades 30 are switched by a switching valve (three-way valve) 844, but other configurations are also possible. That is, as described above, solution L is supplied to reaction chamber 16 by gas G. Therefore, gas G that has been supplied to transport solution L may be continuously supplied to reaction chamber 16, thereby rotating agitator blades 30 by gas G.

[0063] The embodiments disclosed herein are illustrative in all respects and are not restrictive. The technical scope of the present invention is not limited to the above-described embodiments, and includes all modifications within the scope of equivalents to the configurations described in the claims. [Explanation of symbols]

[0064] 10 Reaction vessel 15 Container body 16 Reaction Chamber 17 Supply port 18 Outlet 20 Liquid delivery means 30 stirring blade 31 Central part 32 Wing 33 Straight section 34 Contact area 35 Hollow Part 39 Contacted surface 85 Regulator (adjustment part) 90 Piping 151 Lower 152 Upper 161 Central side 162 Side wall 163 Top surface 164 Bottom surface 391 recess B. Carrier L solution

Claims

1. a vessel body having a reaction chamber for accommodating a carrier and storing a solution; a supply port provided at a lower portion of the container body and through which a gas is supplied; an outlet provided at an upper portion of the container body for discharging gas; a stirring blade that is provided in the reaction chamber and is suspended in the solution and rotated by the gas supplied from the supply port; A reaction vessel having:

2. The stirring blade is The center and a plurality of blade portions extending from the central portion toward a side wall of the reaction chamber; a linear portion extending from the central portion toward an upper surface side or a lower surface side of the reaction chamber; and the linear portion has a contact portion that can come into contact with the upper surface or the lower surface of the reaction chamber; The reaction vessel according to claim 1 .

3. The contact portion has a spherical shape or a tapered shape. The reaction vessel according to claim 2.

4. an upper surface or a lower surface of the reaction chamber has a contacted surface with which the contact portion comes into contact; The contacted surface has a recess into which the contact portion is inserted. The reaction vessel according to claim 2 or 3.

5. The stirring blade has a hollow portion. The reaction vessel according to claim 1 or 2.

6. The stirring blade is The center and three or more blade portions extending from the central portion toward the sidewall of the container body and arranged at equal intervals around the central portion; 10. The reaction vessel of claim 1, comprising:

7. a liquid delivery means for selectively delivering a plurality of types of solutions; a reaction vessel for storing the solution transported by the liquid transport means and for carrying out a reaction using the solution; and The reaction vessel is the reaction vessel according to claim 1, the liquid delivery means has a pipe connected to the supply port, and the pipe has an adjusting unit that adjusts the flow rate of the gas. Synthesizer.

8. A gas having a pressure higher than atmospheric pressure is supplied to the reaction vessel through the supply port. The synthesis apparatus according to claim 7 .

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