Skin external preparation

Abstract

To provide a skin external preparation having combined effects of enhancing skin moisture and inhibiting production of inflammatory substances, capable of exhibiting the effects of refining skin grain, preventing and improving dryness-induced wrinkles, improving transparency and gloss of the skin, normalizing turnover of the skin, and preventing and improving dullness, thereby preventing and improving poor condition and unhealthiness of the skin.SOLUTION: The present invention provides a skin external preparation comprising an extract of leaves of Citrus tachibana as an active ingredient.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an external skin preparation containing, as an active ingredient, a component derived from a plant of the Rutaceae family, which has excellent skin physiological activity and biological safety. [Background technology]

[0002] Traditionally, causes of skin disorders and unhealthy skin have been known to include active oxygen induced by sunlight (ultraviolet rays) or chemical substances (nitrogen compounds, sulfur compounds, etc.) contained in exhaust gases, environmental changes such as dryness, and hormonal imbalances in the body due to stress.

[0003] To date, various moisturizers (natural moisturizing factors [NHFs], glycerin, polyhydric alcohols, sodium lactate, etc.), antioxidants (vitamin E, astaxanthin, polyphenols, etc.), and skin-whitening agents (placenta, ellagic acid, chamomile extract, etc.) have been proposed to prevent and improve skin disorders and ill health, but the ingredients that are effective in preventing and improving skin disorders and ill health were not known. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Publication No. 08-337534 [Patent Document 2] Japanese Patent Application Publication No. 11-246336 [Patent Document 3] Japanese Patent Application Laid-Open No. 2007-031315 Summary of the Invention [Problem to be solved by the invention]

[0005] In view of the problems of the prior art, the present inventors have conducted extensive research to find new active ingredients derived from natural products that are safe for the skin. As a result, they have discovered that an extract from the leaves of Tachibana (Tachibana tachibana), a plant of the genus Citrus in the family Rutaceae, has excellent effects of improving moisturizing, transparency, luster, and barrier function, normalizing skin turnover, preventing and improving dullness, and suppressing inflammation, and thus preventing and improving skin disorders and ill health.

[0006] The use of extracts from the pericarp of Tachibana as ingredients in topical skin preparations has been disclosed in, for example, Patent Documents 1 to 3. However, it was not known that extracts from Tachibana leaves have the effects of improving moisturizing, transparency, luster, and barrier function, preventing and improving dullness, normalizing skin turnover, and suppressing inflammation. [Means for solving the problem]

[0007] The present invention is a topical skin preparation containing an extract of Tachibana leaves. The extract of Tachibana leaves of the present invention is characterized by containing polymethoxyflavonoids. [Effects of the Invention]

[0008] The present invention provides an external skin preparation that uses an extract of Tachibana leaves as an active ingredient, and has the effects of improving moisturization, transparency, luster, and barrier function, normalizing skin turnover, preventing and improving dullness, and suppressing inflammation, thereby preventing and improving skin disorders and ill health. [Brief explanation of the drawings]

[0009] [Figure 1] FIG. 1 shows the results of an analysis of polymethoxyflavonoids contained in the extract according to the present invention. [Figure 2] FIG. 1 shows the results of analysis of polymethoxyflavonoids contained in the extract according to the present invention and polymethoxyflavonoids contained in the comparative extract. [Figure 3] FIG. 1 is a graph showing the relationship between stress score and stratum corneum moisture content. [Figure 4] FIG. 1 is a staining diagram showing the effect of the extract according to the present invention on the stratum corneum under stress. [Figure 5] FIG. 1 is a staining diagram showing the effect of the extract according to the present invention on the stratum corneum. [Figure 6] FIG. 1 is a staining diagram showing the effect of the extract according to the present invention on amino acids in the stratum corneum. DETAILED DESCRIPTION OF THE INVENTION

[0010] The tachibana used in the present invention is a tachibana (scientific name: Citrus tachibana) of the genus Citrus in the family Rutaceae. The plant species is not particularly limited, but examples include Yamato tachibana (Yamato tachibana). The parts used for extraction are the leaves, the above-ground parts including the leaves, or the whole plant.

[0011] To prepare the extract, first, the part of the Tachibana plant to be used for extraction is washed with water if necessary to remove foreign matter, and then either left as is or dried, and then shredded or crushed as necessary, and then brought into contact with an extraction solvent for extraction. Extraction can be carried out by contacting with the extraction solvent using a conventional method such as immersion, but steam distillation or supercritical extraction can also be used instead of immersion.

[0012] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, 1,3-butylene glycol, 1,3-propanediol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these can be used alone or in combination.

[0013] Among the above-mentioned extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoints of the efficacy of the extract obtained, as well as skin irritation, and the wide range of applications for topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals, etc.). Preferred examples of hydrophilic solvents include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol, 1,3-propanediol, and glycerin) alone, or a mixed solvent of water and a lower alcohol (particularly ethanol), or a mixed solvent of water and a polyhydric alcohol (particularly 1,3-butylene glycol, 1,3-propanediol, and glycerin).

[0014] When a mixed solvent is used, the mixing ratio is preferably in the range of, for example, a volume ratio (same hereinafter) of 1:99 to 99:1 for a mixed solvent of water and 1,3-butylene glycol or 1,3-propanediol, 1:25 to 25:1 for a mixed solvent of water and ethanol, or 1:1 to 99:1 for a mixed solvent of water and glycerin.

[0015] The weight ratio of the part of Tachibana leaf to be used to the extraction solvent is preferably 1:1 to 1:100.

[0016] When preparing the extract, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, the extraction solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0017] The extraction conditions, such as extraction temperature and extraction time, vary depending on the type and pH of the solvent used. For example, when water or 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent, the temperature is generally in the range of 0°C to 100°C, and the extraction time is 1 to 168 hours (1 hour to 1 week).

[0018] Prior to or in parallel with the extraction treatment of the present invention, the extract may be subjected to hydrolysis treatment as necessary.

[0019] The extract prepared as described above may be used as an ingredient in a topical skin preparation as is, generally after adjusting the pH to 3 to 8, or may be used at a desired concentration by concentrating under reduced pressure, etc. The extract may also be dried by a conventional method such as spray drying.

[0020] Furthermore, the extraction procedure of the present invention is preferably carried out under conditions of a solvent, extraction temperature and time that allow extraction of polymethoxyflavonoids (polymethoxyflavanones and polymethoxyflavones) contained in Tachibana leaves.

[0021] Examples of topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals) containing the extract of the present invention include, but are not limited to, emulsions, creams, lotions, essences, packs, lipsticks, foundations, sheet masks, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, hair shampoos, cleansing cosmetics such as soaps, hair growth agents, and bath additives.

[0022] The amount of the extract according to the present invention to be incorporated into a skincare topical preparation is generally 0.00001 to 5.0 wt %, preferably 0.0001 to 1.0 wt %, in terms of the solid content of each extract. When incorporated into a hair topical preparation, the amount is generally 0.00001 to 5.0 wt % (solid content weight %, the same applies hereinafter) in terms of the solid content of each extract, preferably 0.0001 to 3.0 wt %.

[0023] When the extract of the present invention is incorporated into topical skin preparations (cosmetics, quasi-drugs, topical pharmaceuticals, etc.), ingredients used in topical skin preparations, such as oily ingredients, surfactants (synthetic or natural), moisturizers, thickeners, emulsifiers or emulsifier aids, preservatives / bactericides, powder ingredients, UV absorbers, antioxidants, pigments, fragrances, anti-wrinkle agents, and other physiologically active ingredients, can be appropriately incorporated as needed. Furthermore, as long as the efficacy and characteristics of the extract of the present invention are not impaired, it is also acceptable to incorporate the extract of the present invention in combination with other physiologically active ingredients into topical skin preparations.

[0024] Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, and paraffin wax. and squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0025] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0026] Examples of emulsifiers and / or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0027] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid and its derivatives, hyaluronic acid fermentation liquid, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, collagen peptides, NMF-related substances, lactic acid, urea, higher fatty acid octyldodecyl, seaweed extract, estradiol, and various amino acids and their derivatives.

[0028] Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0029] Examples of anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0030] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, and ammonium bromide. Examples include chrysoquinolinium, resorcinol, jamal (imidazolidinyl urea), isopropyl methylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, concentrated benzalkonium chloride solution 50, essential oils such as peppermint oil and eucalyptus oil, bark distillate, radish fermented liquid, ethanol derived from plants such as sugar cane and corn, or 1,3-butylene glycol.

[0031] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0032] Anti-acne agents include, for example, sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0033] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0034] Examples of ultraviolet absorbers include ethyl para-aminobenzoate, ethylhexyl para-dimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl para-methoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0035] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, ascorbic acid or its salts or derivatives, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A or its derivatives (retinol palmitate, etc.), and the like.

[0036] Examples of whitening agents include one or more selected from kojic acid or a derivative thereof, ascorbic acid or a derivative thereof, hydroquinone or a derivative thereof, ellagic acid or a derivative thereof, nicotinic acid or a derivative thereof, resorcinol derivatives, tranexamic acid or a derivative thereof, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid or a derivative thereof, vitamin E or a derivative thereof, α-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white mustard extract or hydrolyzed products thereof.

[0037] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate; Ascorbic acid sugar derivatives such as ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, etc.; 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is hexanoyl, octanoyl, decanoyl, etc.); L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc.; L-ascorbic acid tetrafatty acid esters such as 3-isopalmitate, ... -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), Examples of tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), and tranexamic acid amides (e.g., tranexamic acid methylamide). Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include nicotinamide (niacinamide) and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0038] Anti-wrinkle agents include, for example, vitamin A or a derivative thereof, vitamin E or a derivative thereof (tocopherol acetate, etc.), ascorbic acid or a derivative thereof (ascorbic acid glucoside, 3-O-ethyl ascorbic acid, ascorbic acid phosphate magnesium salt, etc.), pantothenyl alcohol, tranexamic acid, nicotinamide, allantoin, etc.

[0039] Furthermore, it is also possible to use in combination components derived from natural products such as the following plants or microorganisms: collagen or hydrolysates thereof, yeast extracts or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraea plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbosacaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, and Anacardiaceae plants. Examples of suitable extracts include extracts of one or more plants selected from the family Garcinia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Mirrataceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0040] As ingredients derived from grass plants, particularly preferred are rice leaf hydrolysate, rice extract hydrolysate, rice bran extract hydrolysate, germinated brown rice hydrolysate, rice fermentation liquid, sake lees extract derived from sake, bamboo shoot skin extract from Madake or Moso bamboo, and fermented Job's tears seed. Furthermore, as Brassicaceae plants, particularly preferred are extracts of seeds of Hakugai, Ogai, or Kokugai, or hydrolysates or fermented products thereof. Furthermore, as ingredients derived from Theaceae plants, particularly preferred are green tea (Yabukita, Samidori, Asahi, Goko, Ujimidori, Kyomidori, Ujihikari, Samidori, Benifuuki, etc.) and black tea (Darjeeling, Assam, Ceylon, Earl Grey, Honey Black Tea, etc.). As ingredients derived from Rosaceae plants, preferred are Damask rose flower extract, peach flower, leaf, or immature fruit extract, strawberry flower extract, and cherry blossom flower or leaf extract. Furthermore, as components derived from Paeoniaceae plants, extracts of peony root or flower, and peony flower are preferred. As components derived from Amaranthaceae plants, Salicornia extract is particularly preferred. As components derived from Zosteraea plants, extracts of Zostera marina or Zostera kohlrauss are particularly preferred. As components derived from Leguminosae plants, extracts of white soybeans or black soybeans or their hydrolysates, fermented soy milk, adzuki bean extract, red clover extract, and pueraria lobata root extract are particularly preferred. As components derived from Asteraceae plants, burdock root extract, sunflower sprout extract, Arctium gracilis extract, arnica extract, and chamomile flower extract are particularly preferred. As components derived from Malvaceae plants, fermented products of hibiscus, rose of sharon, or hibiscus are preferred. As components derived from Gentianaceae plants, gentian extract is preferred. As components derived from Lamiaceae plants, perilla extract and barberry fruit extract are preferred. As a component derived from a plant of the Nelumbo family, particularly preferred is a lotus flower or lotus seed extract or a fermented lotus seed product. As a component derived from a plant of the Cucurbitaceae family, particularly preferred is a loofah extract. As a component derived from a plant of the Araliaceae family, a ginseng extract or fermented product is preferred. As a component derived from a plant of the Solanaceae family, an extract of eggplant (long eggplant, water eggplant, rice eggplant, Kamo eggplant, etc.) is included. As a component derived from a plant of the Bignoniaceae family, a Pau d'Arco bark extract is preferred. As a component derived from a plant of the Actinidiaceae family, an immature kiwi extract is preferred.As components derived from plants of the Moraceae family, mulberry bark extract, mulberry fruit extract, and fig fruit or bark extract are preferred. As components derived from plants of the Rhamnaceae family, jujube fruit extract is preferred. Furthermore, as components derived from plants of the Iridaceae family, saffron is preferred. As components derived from plants of the Campanulaceae family, an extract or hydrolysate of Codonopsis globulus root is preferred. As components derived from plants of the Anacardiaceae family, mango fruit extract is particularly preferred. As components derived from plants of the Garciniaceae family, mangosteen fruit extract is particularly preferred. Furthermore, as components derived from plants of the Valenciaceae family, cherimoya fruit extract is preferred. As components derived from plants of the Rutaceae family, extracts of mandarin oranges, bergamot fruit extracts, grapefruit or banpeiyu fruit (including immature fruits), extracts containing flavonoids and their glycosides contained in plants such as grapefruit or hassaku, or Japanese pepper seed extract are preferred. As components derived from plants of the Liliaceae family, extracts of daylily, daylily, Casablanca lily, Madonna lily, or Japanese lily are preferred. As components derived from plants of the Crassulaceae family, an extract or fermented product of Rhodiola rosea is particularly preferred. As components derived from plants of the Oleaceae family, jasmine flower extract is particularly preferred. As plants of the Cupressaceae family, juniper fruit extract is particularly preferred. As components derived from plants of the Myrtaceae family, guava leaf extract is particularly preferred. As plants of the Orchidaceae family, an extract of Bletilla sieboldii root (white orchid) is particularly preferred. As components derived from plants of the Convolvulaceae family, sweet potato extract or a fermented product thereof, or sweet potato shochu lees extract or a fermented product thereof is particularly preferred. As components derived from seaweeds of the Laminaceae family, kelp extract is particularly preferred, as components derived from seaweeds of the Mirinaceae family, Katamenkirinsai extract is preferred, and as components derived from seaweeds of the Ulva family, Ulva pertusa extract is particularly preferred. As the component derived from Funoriaceae seaweed, Funori extract is particularly preferred.

[0041] The present invention will now be described in more detail with reference to Production Examples, Formulation Examples, and Test Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0042] Preparation Example 1: Preparation of extract Tachibana leaves of the genus Citrus in the family Rutaceae were dried, and 1000 g of 1,3-butylene glycol was added to 100 g of the dried product. The pH was adjusted to a range of 4 to 6, and the extraction temperature was adjusted to a range of 40 to 90° C. while stirring and extracting for 3 hours. This was filtered, and purified water and 1,3-butylene glycol were added to the filtered extract solution, yielding 8,400 g of a pale yellow Tachibana leaf extract solution (solid concentration 0.20%).

[0043] The preparation of the extract of the present invention is not limited to Preparation Example 1. For example, purified water, propanediol, glycerin, or ethanol may be used alone as the extraction solvent, or a mixture of purified water with propanediol, glycerin, or ethanol may be used. Furthermore, by appropriately adjusting the extraction time, temperature, and pH, an extract solution containing the following four polymethoxyflavonoids can be obtained.

[0044] The extract of the present invention preferably contains polymethoxyflavonoids contained in Tachibana leaves, such as polymethoxyflavanones and polymethoxyflavones. Examples of polymethoxyflavanones include 5,6,7,3',4'-pentamethoxyflavanone and citromitin, and examples of polymethoxyflavones include nobiletin and tangeretin.

[0045] Example 1. Analysis of components contained in the extract according to the present invention The polymethoxyflavanones and polymethoxyflavones contained in the Tachibana leaf extract (extract of Production Example 1) according to the present invention were analyzed by liquid chromatography (HPLC) under the following conditions. For comparison, a comparative analysis was also carried out with the components contained in the Tachibana peel extract (extract of Comparative Production Example 1 below). [Comparative manufacturing example] The peel of Tachibana (a member of the genus Citrus in the family Rutaceae) was dried, and 1000 g of 1,3-butylene glycol was added to 100 g of the dried product. The pH was adjusted to a range of 4 to 6, and the mixture was stirred and extracted for 3 hours while adjusting the extraction temperature to a range of 40 to 90° C. This was filtered, and purified water and 1,3-butylene glycol were added to the filtered extract solution to obtain 8,800 g of a pale yellow Tachibana peel extract solution (solid concentration 0.2%). [Analysis method] (1) Detector: ultraviolet absorption photometer (measurement wavelength: 280 nm) (2) A stainless steel tube with an inner diameter of 4.6 mm and a length of 25 cm is filled with 5 μm octadecylsilanized silica gel for liquid chromatography. (3) Column temperature: constant temperature around 40°C (4) Mobile phase A: 0.1% formic acid aqueous solution Mobile phase B: methanol Mobile phase delivery: The mixing ratio of mobile phase A and mobile phase B is changed as shown in Table 1 below to control the concentration gradient. [Table 1] JPEG2025123203000002.jpg50101(5) Flow rate: 1 mL per minute

[0046] The analytical results of Example 1 are shown in Figure 1. As shown in Figure 1, peaks (a) to (d) were confirmed in the extract according to the present invention (extract of Production Example 1). Furthermore, as shown in Figure 2, when the extract according to the present invention (extract of Production Example 1) "A" was compared with the extract of the Comparative Production Example "B," specific peaks (a) and (b) were confirmed in the extract according to the present invention "A," which were not observed in the extract of the Comparative Production Example "B."

[0047] Next, the components represented by peaks (a) to (d) were fractionated by HPLC under the following conditions. (1) Detector: ultraviolet absorption photometer (measurement wavelength: 280 nm) (2) A stainless steel tube with an inner diameter of 10 mm and a length of 25 cm is filled with 5 μm octadecylsilanized silica gel for liquid chromatography. (3) Column temperature: constant temperature around 40°C (4) Mobile phase: methanol / water / acetic acid mixture (7000:2997:3) (5) Flow rate: Flow rate: 2mL per minute

[0048] Next, the four fractions obtained by fractionation under the above conditions were analyzed by nuclear magnetic resonance (NMR) spectroscopy to determine their structural formulas. The results are shown below.

[0049] The fraction (a) of peak (a) was identified as a polymethoxyflavonoid "5,6,7,3',4'-Pentamethoxyflavanone" having the structural formula [Chemical Formula 1] from the NMR analysis results shown below. 1 HNMR(CD3OD)δ=7.10(1H, d, J=2.0Hz, H-2') , 7.02(1H, dd, J=8.8Hz, 2.0Hz, H-6') , 6.96(1H, d, J=8.4Hz, H-5') , 6.47(1H, s, H-8), 5.38(1H, dd, J=12.8Hz, 2.8Hz, H-2) , 3.87 (3H, s, OCH3) , 3.85 (3H, s, OCH3) , 3.84 (3H, s, OCH3) , 3.83 (3H, s, OCH3) , 3.74 (3H, s, OCH3), 3.05(1H, dd, J=16.8Hz, 13.2Hz, H-3), 2.69(1H, dd, J=16.8Hz, 2.8Hz, H-3)

[0050] [ka]

[0051] Furthermore, the fraction (b) of peak (b) was confirmed to be a polymethoxyflavonoid "citromitin (5,6,7,8,3',4'-hexamethoxyflavanone)" having the structural formula [Chemical Formula 2] from the NMR analysis results shown below. 1HNMR(CD3OD)δ=7.13(1H, d, J=1.6Hz, H-2'), 7.01(1H, dd, J=8.8Hz, 1.6Hz, H-6'), 6.97(1H, d, J=8.0Hz, H-5'), 5.43(1H, dd, J=12.4Hz, 2.8Hz, H-2), 4.01(3H, s, OCH3), 3.84 (3H, s, OCH3), 3.83 (3H, s, OCH3), 3.81 (3H, s, OCH3), 3.79 (6H, s, OCH3), 3.10 (1H, dd, J=16.8Hz, 12.8Hz, H-3), 2.78 (1H, dd, J=16.8Hz, 2.8Hz, H-3).

[0052] [ka]

[0053] Furthermore, the fraction (c) of peak (c) was confirmed to be a polymethoxyflavone "nobiletin (3',4',5,6,7,8-hexamethoxyflavone)" having the structural formula [Chemical Formula 3] from the NMR analysis results shown below. 1HNMR(CD3OD)δ=7.61(1H, dd, J=8.8Hz, 2.0Hz, H-6'), 7.49(1H, d, J=2.4Hz, H-2'), 7.08(1H, d, J=8.4Hz, H-5'), 6.66(1H, s, H-3), 4.08(3H, s, OCH3), 4.00(3H, s, OCH3), 3.91(3H, s, OCH3), 3.90(3H, s, OCH3), 3.89(3H, s, OCH3), 3.86(3H, s, OCH3).

[0054] [ka]

[0055] Furthermore, the fraction (d) of peak (d) was confirmed to be a polymethoxyflavone "tangeretin (4',5,6,7,8-pentamethoxyflavone)" having the structural formula [Chemical Formula 4] from the NMR analysis results shown below. 1 HNMR(CD3OD)δ=7.95(2H, d, J=8.8Hz, H-2' / 6'), 7.08(2H, d, J=8.8Hz, C3'-H, H-5'), 6.65(1H, s, H-3), 4.08(3H, s, OCH3), 4.00(3H, s, OCH3), 3.90(3H, s, OCH3), 3.87(3H, s, OCH3), 3.86(3H, s, OCH3).

[0056] [ka]

[0057] Test Example 1: PGE2 Metabolic Inhibition Evaluation Test Normal human epidermal cells (NHEK) were seeded onto a 96-well plate and cultured for 24 hours under standard epidermal cell culture conditions. The extract from Production Example 1 was then added as a sample solution, followed by another 24-hour culture. The concentration of the sample solution was adjusted to 1.0% relative to the total volume of the culture medium. As a comparative control, a test group (control group) was prepared in which a culture medium containing only 30% butylene glycol (30BG) at the same concentration was added instead of the sample solution. Next, the cells in the culture medium were irradiated with approximately 50 mJ / cm using a UV-B lamp (Philips TL20W / 12RS). 2 After further culturing for 24 hours, the supernatant was collected. The amount of the inflammatory substance prostaglandin E2 (PGE2) in the collected supernatant was measured using the Prostaglandin E2 ELISA Kit - Monoclonal (Cayman Chemical Company) according to the manual. The difference between the measured values ​​in the UV-B irradiated and unirradiated control groups was taken as 100% inhibition, and the inhibitory effect of the evaluation sample on the synthesis of the inflammatory substance PGE2 was calculated as a relative value.

[0058] The results of Test Example 1 are shown in Table 2. [Table 2] JPEG2025123203000007.jpg36127

[0059] As shown in Table 2, it was confirmed that the extract according to the present invention (the extract of Production Example 1) inhibits the production of an inflammatory substance (PGE2) induced by ultraviolet rays, suggesting that it inhibits skin inflammation.

[0060] Test Example 2: Evaluation test of the expression of factors related to moisturizing, etc. (improvement of moisturizing feeling, etc.) Normal human epidermal cells (NHEK) were seeded onto 24-well plates and cultured for 24 hours under standard epidermal cell culture conditions. The test solution was then added and cultured for an additional 48 hours. The final concentration of the test solution was adjusted to 1.0% of the total medium volume. A control group was also prepared by adding culture medium containing only 30% BG at the same concentration instead of the test solution. Cells from each group were harvested using the commercially available ISOGEN II Reagent (Nippon Gene Co., Ltd.). Total RNA was extracted and purified from the harvested cells according to the instructions provided with the ISOGEN II Reagent. The harvested total RNA was then reverse-transcribed to cDNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) [Takara Bio Inc.]. The synthesized cDNA was used as a sample and was then purified using Thermal Cycler Dice® Real Time System Single (manufactured by Takara Bio Inc.) and SYBR® Premix Ex Taq TM Using a Perfect Real Time (Takara Bio) detector, the expression of various genes and the internal standard GAPDH gene were detected. The test results were obtained by comparing the expression levels of each gene in each test group, with the expression level of the GAPDH gene held constant. The expression level in the control group was set at 100%, and relative values ​​were calculated.

[0061] The results of Test Example 2 are shown in Table 3. [Table 3] JPEG2025123203000008.jpg37154

[0062] As shown in Table 3, it was confirmed that the extract according to the present invention (the extract of Production Example 1) significantly enhanced the expression of filaggrin, caspase-14, and peptidylarginine deiminase 1 (PADI1), which are factors related to moisturizing (e.g., improved moisturizing feeling). This suggests that the extract according to the present invention has the effect of improving skin moisturizing by promoting the synthesis of filaggrin, a protein that is the source of amino acids (natural moisturizing factors: NMFs), which are moisturizing components of skin, and by promoting the expression of caspase-14 and peptidylarginine deiminase 1 (PADI1), which are enzymes involved in the decomposition of filaggrin into amino acids (natural moisturizing factors).

[0063] As described above, the extract according to the present invention has both the effect of improving skin moisture and the effect of suppressing the production of inflammatory substances, and therefore, by using the extract as an active ingredient, it is possible to provide an external skin preparation that improves skin texture, prevents and improves wrinkles caused by dryness, improves skin clarity and luster, and prevents and improves dullness, thereby preventing and improving skin disorders and ill health. Furthermore, since a decrease in natural moisturizing factors in the stratum corneum disrupts skin turnover, the extract according to the present invention is also expected to have the effect of normalizing skin turnover.

[0064] Test Example 3: Evaluation test of polymethoxyflavonoids For the extract of the present invention (extract of Production Example 1), each of the above-mentioned fractions (a) to (d) and a mixture of fractions (a) to (d) were subjected to an evaluation test for the expression of factors related to moisturizing (improvement of moisturizing feeling, etc.) using the same procedure as in Test Example 3.

[0065] The results of Test Example 3 are shown in Table 4. [Table 4] JPEG2025123203000009.jpg47123

[0066] As shown in Table 4, it was confirmed that each polymethoxyflavonoid significantly enhanced the expression of caspase-14, peptidylarginine deiminase-1 (PADI1), and peptidylarginine deiminase 3 (PADI3), which are factors related to moisturizing (e.g., improving moisturizing sensation). This confirmed that the polymethoxyflavonoid contained in the extract according to the present invention (extract of Production Example 1) contributes to the effect of promoting the expression of caspase-14, peptidylarginine deiminase 1 (PADI1), and peptidylarginine deiminase 3 (PADI3), which are enzymes involved in the decomposition of filaggrin, a protein that is the source of amino acids (natural moisturizing factors) that are moisturizing components of skin, into amino acids (natural moisturizing factors). Regarding filaggrin expression, while each polymethoxyflavonoid alone was not found to have the effect of enhancing expression, it was confirmed that combining fractions containing these four polymethoxyflavonoids (fractions (a) to (d)) produced an effect of enhancing filaggrin expression. Furthermore, it was also confirmed that polymethoxyflavonoids enhance the expression of transglutaminase-1 (TGM1). Transglutaminase-1 is an enzyme that crosslinks proteins during the formation of the cornified envelope that lines the cell membrane of stratum corneum cells. Therefore, it is suggested that the extract of the present invention improves the stratum corneum barrier function by enhancing the expression of transglutaminase-1, leading to skin that is clear and moisturized.

[0067] Test Example 4: Evaluation test of stratum corneum moisture content [Sample preparation] A sample lotion was prepared by mixing 1.0% of the extract solution according to the present invention (extract solution of Production Example 1), 5.0% of 1,3-butylene glycol, 0.2% of paraben, and the remainder purified water, and a control lotion was prepared having the same composition as the sample lotion except that 30% of 1,3-butylene glycol was added instead of the extract solution of Production Example 1. [Test method] The sample lotion and control lotion were applied to each half of the face of each subject, twice a day, morning and evening, for two weeks (the skin care lotion that the subject normally used was replaced with the test lotion of Test Example 5). The stratum corneum moisture content values ​​of each subject at the start of the test and two weeks later were analyzed by dividing the subjects into a low stress group (4 subjects) and a high stress group (4 subjects) based on the score obtained from a self-stress level check (Shiranui method). The results are shown as relative values, with each subject's initial value set at 100.

[0068] The results of Test Example 4 are shown in Table 5. [Table 5] JPEG2025123203000010.jpg66122

[0069] The relationship between the stress score of the subjects and the moisture content of the stratum corneum is shown in Figure 3. As shown in Figure 3, it was confirmed that the moisture content of the stratum corneum decreases when the skin is subjected to stress. The results of the evaluation of the improvement in moisture content of the stratum corneum are shown in Table 5. It was found that applying the extract of the present invention (the extract of Production Example 1) to the skin resulted in a high improvement rate, particularly in the subjects in the high stress group, and it was confirmed that the extract of the present invention can significantly restore the moisture content of the stratum corneum that has decreased due to stress.

[0070] Test Example 5-1. Evaluation using a three-dimensional skin model A three-dimensional skin model, LabCyte Epi-Model 6D (J-tec), was cultured using the specified method. After 24 hours, each sample was added to the stratum corneum and cultured for 3 hours, after which the sample was removed by suction. Meanwhile, 0.3 μM cortisol was added to the dedicated culture medium at the same time as the sample was added. After 3 hours of culture, the medium was replaced with a cortisol-free medium (normal group), and the culture was continued in the cortisol-containing medium (stress group). Sample addition and medium replacement were performed daily. This treatment was repeated for 8 days, and the culture was terminated. After culture, the tissue was fixed, sectioned, and stained with hematoxylin-eosin (HE) for observation. The thickness of the stratum corneum and viable cell layer was measured. The ratio of the stratum corneum thickness to the viable cell layer thickness was calculated as the results of Test Example 5.

[0071] Test Example 5-2. Evaluation of moisturizing protein synthesis using a three-dimensional skin model The three-dimensional skin model, LabCyte Epi-Model 6D (J-tec), was cultured using the specified method. After 24 hours, each sample was added to the stratum corneum and cultured for three hours, after which it was removed by suction. Meanwhile, 3 μM cortisol was added to the dedicated culture medium at the same time as the sample was added, and the culture medium was changed daily. After culturing, the three-dimensional skin model was fixed in formalin, embedded in paraffin, and sectioned. Each section was immunostained using anti-transglutaminase-1 antibody and fluorescently labeled antibody, and photographed under a fluorescence microscope.

[0072] The results of Test Example 5-1 are shown in Table 6, and the results of Test Example 5-2 are shown in Figure 4. [Table 6] JPEG2025123203000011.jpg30131

[0073] As shown in Table 6 and Figure 4, the addition of the stress substance cortisol decreased the ratio of stratum corneum thickness to the thickness of the viable cell layer, resulting in a thinner stratum corneum. It was also confirmed that the amount of transglutaminase-1 synthesized in the stratum corneum was significantly reduced. Furthermore, it was confirmed that the stratum corneum in the group to which the extract of the present invention (the extract of Production Example 1) was added was thicker than that in the stress group, confirming that the amount of transglutaminase-1 synthesized had recovered. Transglutaminase-1 has the function of cross-linking proteins in the stratum corneum, improving skin barrier function. This suggests that the extract of the present invention has the effect of suppressing stress-induced deterioration of barrier function and maintaining skin in a healthy state.

[0074] Test Example 6: Evaluation test of epidermal cell gene expression Normal epidermal cells (NHEK) were seeded onto 24-well plates and cultured under standard epidermal cell culture conditions for 24 hours. The extract from Preparation 1 was added to the culture medium as a sample solution along with 1 μM cortisol and 1.8 mM calcium chloride, and the epidermal cells were cultured under the same conditions. The sample solution was added to the culture medium to a final concentration of 1.0%. A control group was cultured with 1 μM cortisol and 1.8 mM calcium chloride, plus 30% BG solution at the same concentration instead of the sample solution. After 24 hours of culture, cells from each test group were harvested using the commercially available ISOGEN II Reagent (Nippon Gene Co., Ltd.). Total RNA was extracted and purified from the harvested cells according to the instructions provided with the ISOGEN II Reagent. The harvested total RNA was reverse-transcribed using a designated kit (PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time) [Takara Bio Inc.]) to synthesize cDNA. Using the synthesized cDNA as a sample, expression of various genes and expression of the internal standard G3PDH gene were detected using Thermal Cycler Dice® Real Time System Single (manufactured by Takara Bio Inc.) and SYBR® Premix Ex Taq™ II (Perfect Real Time) (manufactured by Takara Bio Inc.). The test results were obtained by comparing the expression levels of each gene in each test group when the expression level of the G3PDH gene was kept constant. In this test system, the expression level of each gene in the control group was set to 100, and the relative expression level of that gene in other test groups was calculated.

[0075] The results of Test Example 6 are shown in Table 7. [Table 7] JPEG2025123203000012.jpg29162

[0076] As shown in Table 7, the extract of the present invention (the extract of Preparation Example 1) was confirmed to significantly enhance the expression of factors related to moisturizing (e.g., improved moisturizing sensation), such as filaggrin, caspase-14, peptidylarginine deiminase 1 (PADI1), claudin-1, and transglutaminase 1 (TGM1), in the presence of the stress substance cortisol. This suggests that the extract of the present invention is effective in maintaining skin moisture and barrier function, even in skin with reduced moisture and barrier function due to stress, by promoting the synthesis of filaggrin, a protein that is the source of amino acids (natural moisturizing factors), and by promoting the expression of caspase-14 and peptidylarginine deiminase 1 (PADI1), enzymes involved in the degradation of filaggrin to amino acids (natural moisturizing factors), as well as claudin-1 and transglutaminase 1 (TGM1).

[0077] Test Example 7: Evaluation test of stratum corneum smoothness Two weeks after application of the sample of Test Example 4, stratum corneum samples were collected from each test site using tape stripping. The collected tapes were immersed in a 0.8% gentian violet / 0.2% brilliant green solution for 5 minutes and then rinsed with running water to stain the stratum corneum. Microscopic observation was performed to evaluate the smoothness of the stratum corneum (whether the stratum corneum peeled evenly or in layers). Figure 5 shows the test results for the stratum corneum in the control lotion application area (a) and the lotion application area containing the sample solution (b). As shown in Figure 5, the stratum corneum in the control lotion application area was stained darkly in some areas, indicating that the stratum corneum peeled in layers, whereas the stratum corneum in the sample lotion application area was stained evenly, indicating that the stratum corneum peeled evenly. This suggests that the extract of the present invention has the effect of smoothing the stratum corneum and homogenizing the reflection of light entering the skin, thereby improving skin brightness.

[0078] Test Example 8: Evaluation test of stratum corneum amino acid content Two weeks after application of the sample in Test Example 4, the stratum corneum of each test site was sampled using the tape stripping method. A 0.1% ninhydrin / ethanol solution was sprayed onto the sampled tape and allowed to stand on a hot plate at approximately 150°C for 10 minutes, and the blue coloring due to amino acids was observed. Figure 6 shows the test results, showing the appearance of the stratum corneum in the control lotion application area (a) and the appearance of the stratum corneum in the lotion application area containing the sample solution (b). As shown in Figure 6, the stratum corneum in the sample lotion application area was stained more deeply than the stratum corneum in the control lotion application area. This suggests that the extract of the present invention has the effect of increasing amino acids, one of the natural moisturizing factors in the stratum corneum, and improving skin to a smoother, more moisturized state.

[0079] The extract of the present invention is expected to have even more significant anti-aging effects when used in combination with other ingredients (niacinamide, tranexamic acid, pantothenyl alcohol, dipotassium glycyrrhizinate, tocopherol acetate, ascorbic acid derivatives, ceramide, collagen, hyaluronic acid, etc.).

[0080] Prescription example 1. Lotion [Ingredients] Part Extract of Preparation Example 1 0.5 Squalane 0.2 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Tocopherol acetate 0.02 Dipotassium glycyrrhizinate 0.5 Monoammonium glycyrrhizinate 0.5 Isopropylmethylphenol 0.1 1,3-butylene glycol 5.0 Sodium citrate 0.2 Methylparaben 0.1 Purified water (enough to make the total volume 100 parts)

[0081] Prescription example 2: Lotion [Ingredients] Part Extract of Preparation Example 1 1.0 Glyceryl Caprylate 3.0 Polyglyceryl-10 Laurate 3.0 Cetyl alcohol 2.0 Behenyl Alcohol 2.0 Methylparaben 0.1 Niacinamide 5.0 Glycyrrhizic acid 0.5 Resorcinol 0.1 Zinc Oxide 2.0 dl-camphor 0.5 Glycerin 2.0 1,3-butylene glycol 5.0 Potassium hydroxide 0.5 Purified water (enough to make the total volume 100 parts)

[0082] Prescription example 3: Lotion [Ingredients] Part Extract of Preparation Example 1 0.5 Jojoba oil 1.0 Polyoxyethylene (5.5) Cetyl Alcohol 5.0 Methylparaben 0.1 Ascorbic Acid Glucoside 2.0 Niacinamide 5.0 ε-aminocaproic acid 0.1 Sulfur 0.2 Estradiol 0.1 Pyridoxine hydrochloride 0.5 Glycerin 5.0 1,3-butylene glycol 5.0 Sodium citrate 0.2 Sodium metabisulfite 0.2 d-Camphor 0.1 Purified water (enough to make the total volume 100 parts)

[0083] Prescription example 4: Emulsion [Ingredients] Part Extract of Preparation Example 1 1.5 Squalane 5.0 Cyclopentanesiloxane 1.0 Hexalan 3.0 Hexyldecyl Isostearate 1.0 Caprylic / Capric Triglyceride 1.0 Polyglyceryl-10 Laurate 5.0 Polyglyceryl-10 Isostearate 5.0 Ascorbyl dipalmitate 15.0 Hydrogenated soy lecithin 1.5 Ascorbic acid phosphate magnesium salt 3.0 Arbutin 3.0 Potassium hydroxide 0.5 Glycerin 3.0 1,3-butylene glycol 2.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Tremella fuciformis polysaccharide 0.2 Sodium hyaluronate 0.01 Tocopherol acetate 0.3 Tocopherol nicotinate 0.1 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Isopropylmethylphenol 0.1 Water-soluble collagen 1.0 Hydrolyzed Collagen 1.0 Acetyl hyaluronic acid 0.1 Purified water (enough to make the total volume 100 parts)

[0084] Prescription example 5. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of L-ascorbic acid 2-glucoside was used in place of 2.0 parts of ascorbic acid phosphate magnesium salt.

[0085] Prescription example 6. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of tranexamic acid were used in place of 2.0 parts of ascorbic acid phosphate magnesium salt and potassium hydroxide.

[0086] Prescription example 7. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of ascorbic acid phosphate magnesium salt was replaced with 3.0 parts of 3-O-ethyl ascorbic acid.

[0087] Prescription example 8. Emulsion An emulsion was obtained in the same manner as in Formulation Example 4, except that 2.0 parts of ascorbic acid phosphate magnesium salt and 5.0 parts of niacinamide were used in place of potassium hydroxide.

[0088] Prescription example 9. Cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Behenyl Alcohol 1.0 Stearyl Alcohol 1.0 Candelilla Wax 1.0 Lactic acid bacteria fermented rice 2.0 Soybean-derived hydrogenated lecithin 0.5 Extract of Preparation Example 1 1.0 Carboxyvinyl Polymer 1.0 Sodium alginate 1.0 Glycerin 4.0 Preservatives 5.2 pH adjuster (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0089] Prescription example 10. Cream [Ingredients] Part Extract of Preparation Example 1 1.0 Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Hexyldecyl Isostearate 5.0 Dioctyldodecyl / Phytosteryl Lauroyl Glutamate / Behenyl) 5.0 Glyceryl Caprylate 1.0 Glyceryl stearate 1.0 Isostearyl glyceryl 3.0 γ-oryzanol 0.1 Behenyl Alcohol 2.0 Palmitic acid 2.5 D-Pantothenyl Alcohol 3.0 Allantoin 0.1 Riboflavin 0.01 Resorcinol 0.1 Benzalkonium chloride 0.05 Urea 3.0 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Ammonium glycyrrhizinate 0.1 Niacinamide 5.0 Lactic acid bacteria fermented rice 2.0 Hydrogenated lecithin 0.5 Hydrogenated lysolecithin 0.5 Hydrolyzed Collagen 1.0 Xanthan gum 1.0 Zinc oxide 0.5 dl-camphor 0.3 l-menthol 0.5 Purified water (enough to make the total volume 100 parts)

[0090] Example 11. Pack Department Extract of Preparation Example 1 1.0 Dipropylene Glycol 5.0 Polyoxyethylene (60) hydrogenated castor oil 5.0 Cetyl alcohol 3.0 Behenyl Alcohol 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Ammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.1 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.1 Tocopherol acetate 0.5 Tocopherol nicotinate 0.1 D-Pantothenyl alcohol 0.3 Resorcinol 0.1 Sulfur 2.0 Estradiol 0.002 Water-soluble collagen 1.0 Xanthan gum 2.0 Polyglyceryl-6 Myristate 1.0 Potassium cocoyl glutamate 1.0 Hydrogenated Lecithin 3.0 Hydroxylated Lecithin 3.0 Purified water (enough to make the total volume 100 parts)

[0091] Prescription example 12. Hair shampoo [Ingredients] Part 2.0 of the extract of Preparation Example 1 Sodium Laureth Sulfate 10.0 Glyceryl Monostearate 1.0 Coconut oil fatty acid diethanolamide 2.0 Polyoxyethylene (40) hydrogenated castor oil 0.5 Benzalkonium chloride 1.0 Stearyl Alcohol 2.0 Behenyl Alcohol 2.0 Dimethicone 3.0 Allantoin 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sodium salicylate 0.1 Tocopherol acetate 0.1 Pyrithione Zinc 0.3 Benzoic acid 0.2 Triclosan 0.2 Citric acid 0.1 Propylene Glycol 2.0 Purified water (enough to make the total volume 100 parts)

[0092] Prescription example 13. Hair conditioner [Ingredients] Part Extract of Preparation Example 1 1.0 Polyoxyethylene (10) hydrogenated castor oil 1.0 Distearyldimethylammonium chloride 1.5 Stearyltrimethylammonium chloride 2.0 Glyceryl 2-ethylhexanoate 1.0 Benzalkonium chloride 1.0 Cetyl alcohol 3.0 Stearyl Alcohol 1.0 Allantoin 0.1 Isopropylmethylphenol 0.1 Dipotassium glycyrrhizinate 0.1 Salicylic acid 0.1 Sulfur 0.5 Alkylisoquinolinium bromide solution (75%) 0.06 Pyrithione Zinc 0.3 Methylparaben 0.1 Triclosan 0.2 Resorcinol 0.1 Purified water (enough to make the total volume 100 parts)

[0093] Formulation example 14. Cleansing cosmetics [Ingredients] Part Extract of Preparation Example 1 2.0 Potassium cocoyl glycinate 5.0 Glycerin 10.0 Glyceryl Caprylate 1.0 Sodium lauroyl aspartate 10.0 Water-soluble collagen 5.0 Cetyl alcohol 3.0 Myristyl Alcohol 3.0 Isopropyl methyl alcohol 0.1 Allantoin 0.1 Sulfur 0.5 Glycyrrhizic acid 0.1 Dipotassium glycyrrhizinate 0.1 Monoammonium glycyrrhizinate 0.1 β-Glycyrrhetinic acid 0.05 Stearyl Glycyrrhetinate 0.1 Salicylic acid 0.2 Tocopherol acetate 0.2 Triclosan 0.1 Trichlorocarbanide 0.5 Trichlorohydroxydiphenyl ether 0.2 Concentrated Benzalkonium Chloride Solution 50 0.2 Benzalkonium chloride 0.1 Purified water (enough to make the total volume 100 parts)

[0094] Prescription example 15. Sheet mask A sheet mask is obtained by impregnating a nonwoven fabric with the following ingredients. [Ingredients] Part Extract of Preparation Example 1 1.0 Glycerin 3.0 1,3-butylene glycol 2.0 Niacinamide 5.0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Xanthan gum 1.0 Water-soluble collagen 1.0 Sodium hyaluronate 1.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0095] Prescription example 16. Beauty serum [Ingredients] Part Extract of Preparation Example 1 2.0 Sodium hyaluronate 1.0 Water-soluble collagen 1.0 Tranexamic acid 0.1 Ethanol 2.0 Glycerin 5.0 1,3-butylene glycol 5.0 Methylparaben 0.1 Citric acid 0.3 Sodium citrate 0.6 Eucheuma muscaria extract 5.0 Purified water (enough to make the total volume 100 parts)

[0096] Prescription example 17. Cream [Ingredients] Part Olive oil 5.0 Squalane 5.0 Jojoba oil 5.0 Jojoba Wax 1.0 Shea Butter 2.0 Behenyl Alcohol 1.0 Stearyl alcohol 1.5 Candelilla Wax 0.5 Niacinamide 5.0 Extract of Preparation Example 1 0.5 Lactic acid bacteria fermented rice 3.0 Hydrogenated Lecithin 2.0 Eucheuma muscaria extract 2.0 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Glycerin 4.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

Claims

1. A topical skin preparation containing extract of Tachibana leaves.

2. 2. The external skin preparation according to claim 1, wherein the extract of Tachibana leaves contains polymethoxyflavonoids.

Images