Oral composition and method for visualizing protein in oral cavity

Abstract

To provide an oral composition that includes a surfactant and exhibits superior protein agglomeration effect and visibility of protein agglomerates.SOLUTION: The oral composition contains (A) an organic acid, (B) a nonionic surfactant, and (C) a dye with a sulfonyl group, the pH being at most 6.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention relates to an oral composition and a method for visualizing proteins in the oral cavity. [Background technology]

[0002] Oral proteins are known to be essential substances in the process of oral plaque formation. Oral plaque is composed of a wide variety of bacteria, and their metabolic products cause various oral diseases, including bad breath, gingivitis, oral inflammation, and dental caries. Furthermore, oral proteins are known to function as bacterial adhesion factors and agglutinins during the plaque formation process. Therefore, if oral proteins can be aggregated and efficiently removed, plaque formation will be suppressed, which may result in the prevention and improvement of various oral diseases, including bad breath, gingivitis, oral inflammation, and dental caries.

[0003] Mouthwashes containing ingredients capable of effectively aggregating proteins in the oral cavity have been investigated as a promising method for preventing and improving the above-mentioned oral diseases. For example, Patent Document 1 discloses a mouthwash containing Chinese quince or its extract. Patent Document 2 discloses a mouthwash containing tea leaf extract. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Japanese Patent Application Laid-Open No. 2009-256259 [Patent Document 2] Japanese Patent Application Publication No. 2018-90558 Summary of the Invention [Problem to be solved by the invention]

[0005] In order to remove food residue and other contaminants from the oral cavity, it is desirable to add a surfactant to improve cleansing power. However, in conventional techniques, adding a surfactant reduces protein aggregation, reducing the amount of protein aggregates visible when rinsing the mouth with the mouthwash and then spitting it out. As a result, users may feel that the mouthwash is ineffective and stop using it. Therefore, an object of the present invention is to provide an oral composition that contains a surfactant and yet has excellent protein aggregation effects and visibility of protein aggregates. [Means for solving the problem]

[0006] As a result of extensive research, the inventors discovered that the above problems could be solved by using an oral composition containing an organic acid, a nonionic surfactant, and a specific dye, and having a pH of 6 or less, and thus completed the present invention.

[0007] That is, the present invention is achieved by the following [1] to [4]. [1] An oral composition comprising (A) an organic acid, (B) a nonionic surfactant, and (C) a dye having a sulfonyl group, and having a pH of 6 or less. [2] The oral composition according to [1], wherein (C) is a dye having two or more sulfonyl groups. [3] The oral composition according to [1] or [2], wherein (B) comprises at least one selected from polyoxyethylene hydrogenated castor oil and polyoxyethylene alkyl ether. [4] A step of rinsing the oral cavity with the oral composition according to any one of [1] to [3]; and and discharging the oral composition from the oral cavity. A method for visualizing proteins in the oral cavity that has been washed, in which at least a portion of the proteins in the oral cavity that are excreted together with the oral composition are colored by the oral composition. [Effects of the Invention]

[0008] It is possible to provide an oral composition that contains a surfactant and yet has excellent protein aggregation effects and visibility of protein aggregates. DETAILED DESCRIPTION OF THE INVENTION

[0009] The oral composition of the present invention will be described in detail below.

[0010] [Oral composition] The oral composition of the present invention contains (A) an organic acid, (B) a nonionic surfactant, and (C) a dye having a sulfonyl group, and has a pH of 6 or less.

[0011] <(A) Organic acid> The oral composition of the present invention contains an organic acid (A). The organic acid (A) has the effect of aggregating proteins in the oral cavity. The organic acid (A) also functions as a pH adjuster, adjusting the pH of the oral composition. (A) Organic acids include citric acid, malic acid, lactic acid, benzoic acid, acetic acid, tartaric acid, phytic acid, gluconic acid, fumaric acid, butyric acid, and succinic acid. The (A) organic acid may also be an organic acid salt. Examples of organic acid salts include trisodium citrate, sodium hydrogen citrate, sodium lactate, calcium lactate, sodium benzoate, sodium acetate, sodium malate, and monosodium fumarate. The organic acid and organic acid salt may be used alone or in combination of two or more kinds, as long as they are blended so that the oral composition has an acidic pH of 6 or less. Among these, it is preferable to contain citric acid and trisodium citrate.

[0012] The content of (A) organic acid is preferably 0.05% by mass or more, more preferably 0.10% by mass or more, and even more preferably 0.15% by mass or more, based on the total amount of the oral composition. By making the content of (A) organic acid 0.05% by mass or more, the effect of aggregating proteins in the oral cavity is easily exerted. In addition, it is easy to adjust the pH of the oral composition to a predetermined range. The content of (A) organic acid is preferably 0.35% by mass or less, more preferably 0.30% by mass or less, even more preferably 0.25% by mass or less, and particularly preferably 0.20% by mass or less, based on the total amount of the oral composition. By setting the content to 0.35% by mass or less, the demineralization state of teeth is less likely to persist when the oral composition is held in the oral cavity.

[0013] When the oral composition of the present invention contains both an organic acid and an organic acid salt as (A) organic acid, the content of the organic acid is preferably greater than the content of the organic acid salt, from the viewpoint of maintaining the pH of the composition at an appropriate value. When the mass ratio of the contents of the two (organic acid content / organic acid salt content) is α, it is preferable that α<10, more preferably 2<α<7, and even more preferably 2<α<5. When the oral composition of the present invention contains citric acid and trisodium citrate as the (A) organic acid, the content of citric acid is preferably greater than the content of trisodium citrate, from the viewpoint of maintaining the pH of the composition at an appropriate value. When the mass ratio of the contents of the two (citric acid content / trisodium citrate content) is α, it is preferable that 1<α<10, more preferably 2<α<7, and even more preferably 2<α<5.

[0014] <(B) Nonionic surfactant> The oral composition of the present invention contains a nonionic surfactant, which has the effect of cleaning away food residue and other stains adhering to the oral cavity.

[0015] Examples of nonionic surfactants used in the present invention include polyoxyethylene (hereinafter also referred to as POE) hydrogenated castor oil, POE alkyl ethers such as POE cetyl ether and POE stearyl ether, POE·POP alkyl ethers such as polyoxyethylene·polyoxypropylene (hereinafter also referred to as POE·POP) cetyl ether, POE sorbitan fatty acid esters such as POE sorbitan coconut oil fatty acid ester (also referred to as POE coconut oil sorbitan), glycerin fatty acid esters such as polyglyceryl monolaurate, polyethylene glycol fatty acid esters such as polyethylene glycol monostearate, POE·POP block polymers, POE alkylphenyl ethers, POE·POP fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters, and POE phytosterols, and these may be used alone or in combination. Furthermore, from the viewpoint of further suppressing discoloration of the oral composition and having little effect on taste, POE hydrogenated castor oil, POE alkyl ethers such as POE cetyl ether and POE stearyl ether, POE-POP alkyl ethers such as POE-POP cetyl ether, POE sorbitan fatty acid esters such as POE sorbitan coconut oil fatty acid ester (also called POE coconut oil sorbitan), and polyethylene glycol fatty acid esters such as polyethylene glycol monostearate are preferred. Among these, POE hydrogenated castor oil and POE alkyl ethers such as POE cetyl ether and POE stearyl ether are more preferred because they have less effect on taste, and POE cetyl ether is even more preferred. That is, it is more preferred that the nonionic surfactant contains at least one selected from POE hydrogenated castor oil and POE alkyl ether, and even more preferred that it contains POE cetyl ether. Here, the influence on the sense of taste refers to, for example, the influence on bitterness, irritation, oily smell (odor), and the like.

[0016] The content of the nonionic surfactant is preferably 0.01% by mass or more, more preferably 0.03% by mass or more, and even more preferably 0.05% by mass or more, based on the total amount of the oral composition. If the content is 0.01% by mass or more, the effect of cleaning oral dirt is easily obtained. The upper limit of the content of the nonionic surfactant is preferably 2% by mass or less, more preferably 1.5% by mass or less, and even more preferably 1% by mass or less. When the content is 2% by mass or less, protein aggregation is less likely to be inhibited by the nonionic surfactant. The content of the nonionic surfactant is preferably 0.01 to 2% by mass based on the total amount of the oral cavity composition.

[0017] <(C) Dye Having a Sulfonyl Group> The oral composition of the present invention contains a dye having a sulfonyl group. The dye having a sulfonyl group is easily adsorbed to protein aggregates. When the oral cavity is rinsed with the oral composition of the present invention, the dye having a sulfonyl group is adsorbed to stains containing protein aggregates in the oral cavity, thereby improving the visibility of proteins in the oral cavity that are expelled together with the oral composition when the oral composition is expelled from the oral cavity.

[0018] The number of sulfonyl groups in the dye may be one or more, but is preferably two or more, and more preferably three or more, because this increases the amount of sulfonyl groups adsorbed to protein aggregates and makes the stains more visible. The upper limit of the number of sulfonyl groups is not particularly limited, but is, for example, four or less.

[0019] Dyes with sulfonyl groups include Red No. 102 (number of sulfonyl groups: 3), Blue No. 1 (number of sulfonyl groups: 3), Yellow No. 4 (number of sulfonyl groups: 2), Green No. 201 (number of sulfonyl groups: 2), Orange No. 205 (number of sulfonyl groups: 1), Red No. 1 (number of sulfonyl groups: 2), Red No. 2 (number of sulfonyl groups: 3), Red No. 4 (number of sulfonyl groups: 2), Red No. 40 (number of sulfonyl groups: 2), Red No. 101 (number of sulfonyl groups: 2), Red No. 106 (number of sulfonyl groups: 2), and Red No. 227 (sulfonyl groups). Examples of suitable sulfonyl groups include dyes such as Red No. 1 (sulfonyl group: 2), Yellow No. 1 (sulfonyl group: 1), Yellow No. 5 (sulfonyl group: 2), Green No. 1 (sulfonyl group: 2), Green No. 2 (sulfonyl group: 3), Green No. 3 (sulfonyl group: 3), Blue No. 2 (sulfonyl group: 2), Orange No. 1 (sulfonyl group: 1), Orange II (sulfonyl group: 1), Purple No. 1 (sulfonyl group: 2), Brilliant Milling Green (sulfonyl group: 2), and Azure Blue VX (sulfonyl group: 2). Among these, Red No. 102, Blue No. 1, Yellow No. 4, and Green No. 201 are preferred, with Red No. 102 and Blue No. 1 being particularly preferred.

[0020] As mentioned above, dyes having a sulfonyl group are available in a variety of colors, and it is possible to select one that matches the flavor and image of the product.

[0021] The content of the sulfonyl group-containing pigment is preferably 0.000005% by mass or more, more preferably 0.0005% by mass or more, based on the total amount of the oral composition. When the content is 0.000005% by mass or more, the effect of improving the visibility of stains is easily obtained. The upper limit of the content of the sulfonyl group-containing pigment is preferably 0.01% by mass or less, more preferably 0.005% by mass or less. If the content is 0.01% by mass or less, the color of the oral composition will not be too dark, resulting in a good appearance. Furthermore, the risk of staining the oral cavity, the area around the mouth, the bathroom, etc., when rinsing the mouth is likely to be reduced. The content of the dye having a sulfonyl group is preferably 0.0005 to 0.005% by mass relative to the total amount of the oral cavity composition.

[0022] <Other ingredients> The oral composition of the present invention may contain a solvent. From the viewpoint of application to the oral cavity, the solvent used in the oral composition of the present invention is preferably an aqueous solvent, and examples of the aqueous solvent include water, ethanol, and mixtures thereof.

[0023] The solvent of the present invention is preferably water, ethanol, or a mixture thereof. Examples of water that can be used include purified water, ion-exchanged water, and distilled water. When preparing a liquid oral composition, it may contain water, ethanol, a humectant, and the like, depending on the form. A solvent consisting primarily of water is preferred for application to the oral cavity, and it is preferable to include other solvents such as ethanol or a humectant in amounts less than that of water. For example, the amount of ethanol contained in the composition may be 0 to 20% by mass. For example, the amount of water contained in the composition may be 50 to 99% by mass. For example, the amount of humectant contained in the composition may be 0 to 30% by mass.

[0024] The oral composition of the present invention has a pH of 6 or less. If the pH exceeds 6, it is difficult to obtain a protein aggregating effect. From the viewpoint of improving the protein aggregating effect, the pH is preferably 5 or less, and more preferably 4 or less. Furthermore, if the pH is too low, demineralization of teeth is likely to occur when the oral composition is taken into the oral cavity. Therefore, it is important to make it easy for the pH in the oral cavity to return to a normal state after mouthrinsing, and from the viewpoint of preventing continued demineralization, the pH is preferably 3 or higher. The pH of the oral composition is preferably 4 or less, and more preferably 3-4.

[0025] Examples of pH adjusters that can be used to adjust the pH within the above range include the above-mentioned (A) organic acids, as well as hydrochloric acid, sulfuric acid, nitric acid, phosphoric acid, sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, sodium phosphate, sodium hydrogen phosphate, etc. Adjustment using the above-mentioned (A) organic acids is preferred.

[0026] The oral composition of the present invention may contain various other ingredients as long as they do not impair the effects of the present invention.

[0027] For example, thickeners such as hydroxyethyl cellulose, humectants such as glycerin, ethylene glycol, propylene glycol, 1,3-butylene glycol, polyethylene glycol, and polypropylene glycol, sweeteners such as steviaside, xylitol, erythritol, maltitol, sorbitol, saccharin sodium, sucralose, reduced palatinose, and aspartame, foaming agents such as sodium lauryl sulfate, triclosan, isopropylmethylphenol, cetylpyridinium chloride, benzethonium chloride, and chloroform. Examples of the antiseptic agent include disinfectants such as hexidine, enzymes such as proteases, fluoride ion sources such as sodium fluoride, fluorophosphates, and fluoroborates, calcium sources such as calcium phosphate, hydroxyapatite, and calcium gluconate, preservatives such as parahydroxybenzoic acid esters and sodium benzoate, essential oil components such as peppermint oil, mint oil, menthol, carvacrol, eucalyptus oil, eugenol, anethole, cineole, and hinokitiol, or fragrances containing any of these, and herbal medicines such as Phellodendron Bark extract and Angelica acutiloba extract.

[0028] Other ingredients that may be included include lysozyme chloride, dextranase, mutanase, glycyrrhizinate and its derivatives, glycyrrhetinate and its derivatives, azulene, azulene sulfonic acid, dihydrocholesterol, epidihydrocholesterol, allantoin, allantoin chlorohydroxyaluminum, sodium chloride, dl-α-tocopherol acetate, dl-α-tocopherol nicotinate, epsilon aminocaproic acid, tranexamic acid, polyphosphates, thymol, copper chlorophyllin sodium, vitamins, amino acids, etc.

[0029] <Method of manufacturing oral composition> The oral composition of the present invention can be produced by known means, for example, by mixing and stirring the components, and may be heated if necessary.

[0030] <Uses of oral compositions> The oral composition of the present invention is intended primarily for use in the oral cavity and is preferably a liquid composition. The uses of the oral composition of the present invention are not particularly limited, but it can be used, for example, as a mouthwash by adding an appropriate amount to the oral cavity and rinsing. It can also be used as a liquid dentifrice, including those containing abrasives such as calcium hydrogen phosphate, aluminum hydroxide, anhydrous silicic acid, and calcium carbonate as needed. The oral composition of the present invention can also be used as a mouth freshener by spraying it into the oral cavity, or as a mouthwash.

[0031] [Method for visualizing proteins in the oral cavity] The method of the present invention for visualizing proteins in the oral cavity comprises, in this order, a step of rinsing the oral cavity with the oral composition described above and a step of expelling the oral composition from the oral cavity, and is a method for visualizing proteins in the oral cavity that have been washed by coloring at least a portion of the proteins in the oral cavity that are expelled together with the oral composition with the oral composition.

[0032] The oral composition used for washing the oral cavity may be a composition prepared in advance before being placed in the oral cavity, or the components may be mixed in the oral cavity to form the oral composition described above.

[0033] The oral composition of the present invention has an excellent protein aggregating effect, and the dye contained therein is easily adsorbed to protein aggregates. When the oral cavity is rinsed with the oral composition of the present invention, proteins in the oral cavity aggregate, and the dye having a sulfonyl group is adsorbed to stains containing protein aggregates. Therefore, when the oral composition is expelled from the oral cavity, the visibility of the stains in the oral cavity that are expelled together with the oral composition can be improved. [Example]

[0034] The present invention will be further explained below with reference to examples and comparative examples, but the present invention is not limited to the following examples.

[0035] [Preparation of oral composition] Oral compositions were prepared by mixing the components shown in Tables 1 to 4 below in the proportions shown in Tables 1 to 4. The numerical values ​​for each component in Tables 1 to 4 indicate the blending amount (% by mass) of each component relative to the total amount of the oral composition.

[0036] [evaluation] The rate of change in absorbance of the protein, the rate of change in absorbance of the dye, and the amount of dye incorporated relative to the amount of protein were calculated according to the following procedures. <1> 10 mL of the prepared oral composition was placed in a 15 mL centrifuge tube, and 0.3 mL of saliva (a mixture of equal amounts of resting saliva collected from four men and women in their 20s and 30s) was added. The mixture was stirred by hand and then left to stand overnight at room temperature (20°C). <2> <1> The mixture was centrifuged at 3,000 rpm for 10 minutes using a tabletop centrifuge (KUBOTA5200, manufactured by Kubota Manufacturing Co., Ltd.), and the supernatant was removed. <3> 0.5 mL of purified water <2> In addition, the precipitate was dispersed using an ultrasonic disintegrator (ULTRASONIC DISRUPTOR UD-201, manufactured by Tomy Seiko Co., Ltd.) (output: 1, output time: approximately 10 seconds). <4> In a 96-well plate <3> The absorbance was measured using an absorption spectrometer (Multiskan GO, manufactured by Thermo Fisher Scientific Co., Ltd.), and this was defined as the absorbance (a) of the dye. For Red No. 102, Blue No. 1, Yellow No. 4, Green No. 201, Orange No. 205, and Red No. 230, the absorbance measurements were taken at 510 nm, 630 nm, 427 nm, 642 nm, 484 nm, and 517 nm, respectively. <5> Each sample in a 96-well plate <3> 25 μL of the above was added, and 200 μL of BCA reagent (Protein Assay BCA Kit Working Solution, Product No. 297-73101, Fujifilm Wako Pure Chemical Industries, Ltd.) was added thereto, and the mixture was allowed to stand at 37° C. for 30 minutes. <6> The plate was returned to room temperature and the absorbance at 562 nm was measured. <7> specimen <3> 200 μL of purified water was added to 25 μL of the sample, and the absorbance at 562 nm was measured as the background. <6> The value obtained by subtracting this from the value obtained in step 1 was taken as the absorbance (b) of the protein.

[0037] The absorbance (a) of each dye and the absorbance (b) of the protein were calculated as follows, with the formulation without surfactant (control; Reference Example) set to 1. The amount of dye incorporated relative to the amount of protein was calculated from the absorbance change rates of the dye and protein obtained. Protein absorbance change rate = absorbance of sample (b) / absorbance of control (reference example) Dye absorbance change rate = Dye absorbance of sample (a) / Dye absorbance of control (reference example) Amount of dye incorporated relative to the amount of protein = Dye absorbance change rate / Protein absorbance change rate

[0038] Each component of the oral composition shown in the table will be explained below. ((A) Organic acid) Citric acid Trisodium citrate (surfactant) BC-25 Polyoxyethylene cetyl ether (25E.O.), non-ionic surfactant, trade name: NIKKOL BC-25, manufactured by Nippon Surfactant Kogyo Co., Ltd. HC-100 Polyoxyethylene hydrogenated castor oil (100E.O.), non-ionic surfactant, product name: Uniox HC-100, manufactured by NOF Corporation SLS sodium lauryl sulfate, anionic surfactant, product name: NIKKOL SLS, manufactured by Nippon Surfactant Kogyo Co., Ltd. (dye) Red No. 102 Number of sulfonyl groups: 3 Blue No. 1 Number of sulfonyl groups: 3 Yellow No. 4 Number of sulfonyl groups: 2 Green No. 201 Number of sulfonyl groups: 2 Orange No. 205 Number of sulfonyl groups: 1 Red No. 230 Number of sulfonyl groups: 0 (others) ·ethanol

[0039] <Examples 1-1 to 1-5, Comparative Examples 1-1 to 1-4, Reference Example 1-1> The components shown in Table 1 below were mixed in the proportions shown in Table 1 to prepare the oral composition of Example 1-1. The oral compositions of Examples 1-2 to 1-5 and Comparative Examples 1-1 to 1-4 were prepared in the same manner as Example 1-1, except that the type and amount of the surfactant was changed as shown in Table 1. The oral composition of Reference Example 1-1 was prepared in the same manner as Example 1-1, except that no surfactant was added.

[0040] The evaluation results are shown in Table 1. The results in Examples 1-1 to 1-5 and Comparative Examples 1-1 to 1-4 are relative values ​​when the result in Reference Example 1-1 is set to 1.

[0041] [Table 1]

[0042] The results in Table 1 show that the oral compositions of the present invention (Examples 1-1 to 1-5) cause protein aggregation to the same extent as when no surfactant is included (Reference Example 1-1). Furthermore, it was found that the amount of dye incorporated relative to the amount of protein was the same or greater. On the other hand, in the oral composition of the comparative example, which used an anionic surfactant, the amount of protein aggregation was less than when no surfactant was included, and the amount of dye absorbed relative to the amount of protein was also less. Furthermore, it was also confirmed by visual inspection that the visibility of protein aggregates was improved in the Examples compared to the Comparative Examples.

[0043] <Examples 2-1 to 2-4, Comparative Example 2-5, Reference Examples 2-1 to 2-5> The components listed in Table 2 below were mixed in the proportions shown in Table 2 to prepare the oral composition of Example 2-1. The oral compositions of Examples 2-2 to 2-4 and Comparative Example 2-5 were prepared in the same manner as Example 2-1, except that the type of pigment was changed as shown in Table 2. The oral compositions of Reference Examples 2-1 to 2-5 were each prepared in the same manner as Examples 2-1 to 2-4 and Comparative Example 2-5, except that no surfactant was added.

[0044] The evaluation results are shown in Table 2. The results of Examples 2-1 to 2-4 and Comparative Example 2-5 are relative values ​​when the results of Reference Examples 2-1 to 2-5 are set to 1, respectively.

[0045] [Table 2]

[0046] <Example 3-1, Comparative Example 3-1, Reference Example 3-1> The components listed in Table 3 below were mixed in the proportions shown in Table 3 to prepare the oral composition of Example 3-1. The oral composition of Comparative Example 3-1 was prepared in the same manner as Example 3-1, except that the type of surfactant was changed as shown in Table 3. The oral composition of Reference Example 3-1 was prepared in the same manner as Example 3-1, except that no surfactant was added.

[0047] The evaluation results are shown in Table 3. The results of Example 3-1 and Comparative Example 3-1 are relative values ​​when the result of Reference Example 3-1 is set to 1.

[0048] [Table 3]

[0049] <Examples 4-1 to 4-3, Reference Example 4-1> The components shown in Table 4 below were mixed in the proportions shown in Table 4 to prepare the oral composition of Example 4-1. Oral compositions of Examples 4-2 and 4-3 were prepared in the same manner as Example 4-1, except that the type and amount of surfactant were changed as shown in Table 4. An oral composition of Reference Example 4-1 was prepared in the same manner as Example 4-1, except that no surfactant was added.

[0050] The evaluation results are shown in Table 4. The results of Examples 4-1 to 4-3 are relative values ​​when the result of Reference Example 4-1 is set to 1.

[0051] [Table 4]

[0052] The results in Tables 2 to 4 show that the amount of dye incorporated relative to the amount of protein in the oral composition of the present invention is equal to or greater than that in the case where no surfactant is contained. There was also a tendency for the value to increase as the number of sulfonyl groups in the dye increases. On the other hand, in the oral compositions of Comparative Example 2-5, which used a dye without a sulfonyl group, and Comparative Example 3-1, which used an anionic surfactant, the amount of dye absorbed relative to the amount of protein was lower than when no surfactant was included. Furthermore, it was also confirmed by visual inspection that the visibility of protein aggregates was improved in the Examples compared to the Comparative Examples.

Claims

1. An oral composition comprising (A) an organic acid, (B) a nonionic surfactant, and (C) a dye having a sulfonyl group, and having a pH of 6 or less.

2. The oral composition according to claim 1 , wherein (C) is a dye having two or more sulfonyl groups.

3. The oral composition according to claim 1 or 2, wherein (B) comprises at least one selected from polyoxyethylene hydrogenated castor oil and polyoxyethylene alkyl ether.

4. A step of rinsing the oral cavity with the oral composition according to any one of claims 1 to 3; and and discharging the oral composition from the oral cavity. A method for visualizing proteins in the oral cavity that has been washed, in which at least a portion of the proteins in the oral cavity that are excreted together with the oral composition are colored by the oral composition.

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