Auxiliary device for fragmenting collected tissue

Abstract

To provide an auxiliary device for fragmenting a collected tissue in which enzyme solution can be efficiently added in a fragmentation step of a living body-derived tissue.SOLUTION: An auxiliary device for fragmenting a collected tissue comprises: a placing body 20 which places a collected tissue 210; a plurality of protruding parts 30 which are arranged so as to oppose the placing body 20, and extend in a direction toward the placing body 20; a pressing body 50 which is arranged between the placing body 20 and the protruding parts 30, has intermediate porous parts 52 which allow the protruding parts 30 to be inserted and is movable so as to change a distance to the placing body 20; and a handle part 60 to which the protruding parts 30 are connected, and which moves the protruding parts 30 toward the placing body 20.SELECTED DRAWING: Figure 4

Description

[Technical Field]

[0001] The present invention relates to a collected tissue pulverization assist device used in a process of pulverizing tissue collected from a living body. [Background technology]

[0002] In recent years, new regenerative medicines have been developed as a solution to the treatment of severe heart failure. One example is a method in which sheet-shaped cell cultures created using temperature-responsive culture dishes using tissue engineering are applied to the heart surface in cases of severe myocardial infarction, etc. The method using sheet-shaped cell cultures makes it possible to safely transplant large amounts of cells over a wide area, and is particularly useful for treating heart diseases (e.g., heart failure, particularly chronic heart failure) accompanied by myocardial infarction (including chronic heart failure associated with myocardial infarction), dilated cardiomyopathy, ischemic cardiomyopathy, and systolic dysfunction (e.g., left ventricular systolic dysfunction).

[0003] The biological tissue that serves as the material for the sheet-shaped cell culture is collected by excising a specific part of the body. Tissue for producing the sheet-shaped cell culture used in the treatment of heart failure is usually thigh muscle tissue. The collected tissue is subjected to a shredding process to separate viable cells. The separated viable cells are cultured to create the sheet-shaped cell culture. The shredding process is disclosed in Patent Document 1. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] Patent Publication No. 2021-132541 Summary of the Invention [Problem to be solved by the invention]

[0005] In the process of shredding biological tissue, the biological tissue is immersed in an enzyme solution. After treatment with the enzyme solution, the tissue is shredded. In the shredding process, the treatment with the enzyme solution must be carried out efficiently. Furthermore, since the biological tissue is transported immersed in the tissue transport solution, it may have a high water content at the start of the shredding process. This reduces the amount of enzyme solution that can be added to the biological tissue, which may affect the number of viable cells obtained.

[0006] The present invention has been made to solve the above-mentioned problems, and aims to provide a collected tissue mincing assist device that enables efficient addition of an enzyme solution in the process of mincing biological tissue. [Means for solving the problem]

[0007] (1) A collected tissue shredding assisting device for achieving the above-mentioned object comprises a holder for placing the collected tissue, a plurality of protrusions arranged opposite the holder and extending in a direction toward the holder, a pressing body arranged between the holder and the protrusions and having an intermediate hole for inserting the protrusions and movable to change the distance from the holder, and a handle to which the protrusions are connected and which moves the protrusions toward the holder. [Effects of the Invention]

[0008] The collected tissue shredding assist device configured as described above can drain the collected tissue by manipulating the handle to puncture the collected tissue placed on the support body with the multiple protrusions and sandwich the collected tissue between the pressing body and the support body. Therefore, the collected tissue shredding assist device can reduce the moisture content of the collected tissue while forming multiple holes in the collected tissue, allowing the enzyme solution to be efficiently added to the collected tissue.

[0009] (2) In the collected tissue mincing assist device of (1) above, the holder may have a lower hole through which the protrusion is inserted. This allows the protrusion to penetrate the holder, and the protrusion to penetrate the collected tissue to form a through-hole, allowing the enzyme solution to be added to the collected tissue more efficiently.

[0010] (3) In the collected tissue slicing assist device of (1) or (2) above, the holder may be fixed to a shaft portion extending toward the handle portion, and the handle portion may have a handle biasing portion between the holder and the shaft portion for biasing the handle portion in a direction away from the holder. This allows the protrusion puncturing the collected tissue to return to its original position when the handle portion is released.

[0011] (4) In the tissue mincing assist device of (3) above, the shaft portion may have a pressing body biasing portion that biases the pressing body in a direction away from the holder, so that when the handle portion of the tissue mincing assist device is released, the pressing body holding the collected tissue can return to its original position.

[0012] (5) In the collected tissue collec- tion assist device according to any one of (1) to (4) above, the protrusion may have a cutting edge at its tip, thereby reducing the force required to puncture the collected tissue with the protrusion to form a hole.

[0013] (6) The collected tissue slicing assist device according to any one of (1) to (5) above may further include a needle portion disposed opposite the holder and extending toward the holder, the needle portion having a lumen opening at the tip and a length shorter than the protrusion portion. This allows the collected tissue slicing assist device to puncture the collected tissue with the needle portion and directly inject a liquid such as an enzyme solution.

[0014] (7) In the collected tissue slicing assist device of any of (1) to (6) above, the handle portion may have an injection lumen communicating with the lumen of the needle portion and a port portion to which an injection device for injecting a liquid into the injection lumen can be connected. This allows the collected tissue slicing assist device to directly inject a liquid such as an enzyme solution into the collected tissue from the injection device connected to the port portion via the needle portion.

[0015] (8) The collected tissue slicing assist device of any of (1) to (6) above may further comprise a liquid reservoir communicating with the lumen of the needle, and a second handle connected to a pusher inserted into the handle and configured to deliver the liquid in the liquid reservoir to the lumen of the needle. This allows the collected tissue slicing assist device to inject the liquid in the liquid reservoir directly into the collected tissue via the needle by manipulating the second handle while manipulating the handle to insert the needle into the collected tissue. [Brief explanation of the drawings]

[0016] [Figure 1] FIG. 2 is a perspective view of the tissue harvesting assist device according to the present embodiment. [Figure 2] FIG. 4 is an enlarged view showing the shape of the tip of the protrusion. [Figure 3] FIG. 10 is a perspective view of the collected tissue mincing assist device placed in a centrifuge tube. [Figure 4] 10 is a cross-sectional view of the collected tissue shredding assist device placed in a centrifuge tube and the collected tissue placed on the mounting body. FIG. [Figure 5] 5 is a cross-sectional view showing a state in which the handle portion is pressed down from the state shown in FIG. 4 and the support body comes into contact with the pressing body. [Figure 6] 6 is a cross-sectional view showing a state in which the handle portion has been pushed down from the state shown in FIG. 5 and has reached the lowest end. [Figure 7] FIG. 10 is a cross-sectional view of a state in which a collected tissue shredding assist device according to a first modified example is installed in a centrifuge tube and a collected tissue is placed on a mounting body. [Figure 8] 8 is a cross-sectional view showing a state in which the handle portion is pressed down from the state shown in FIG. 7. FIG. [Figure 9] 9 is a cross-sectional view showing a state in which the second handle portion is pressed down from the state shown in FIG. 8. FIG. [Figure 10] FIG. 10 is a cross-sectional view of a collected tissue mincing assist device according to a second modified example, placed in a centrifuge tube. [Figure 11] 10A and 10B are cross-sectional views of the tissue-collecting assist device according to the third modified example, in which (a) shows the device installed in a centrifuge tube with the collected tissue placed on the support body, and (b) shows the device with the handle portion reaching the lowest end. [Figure 12] FIG. 10 is an enlarged view of the vicinity of the tip when the needle portion is disposed within the protrusion portion. DETAILED DESCRIPTION OF THE INVENTION

[0017] Hereinafter, embodiments of the present invention will be described with reference to the drawings. Note that dimensions in the drawings may be exaggerated for convenience of explanation and may differ from actual dimensions. Furthermore, in this specification and drawings, components having substantially the same functional configurations are designated by the same reference numerals, and redundant explanations will be omitted.

[0018] The collected tissue shredding assist device 10 of this embodiment is used to create multiple holes in tissue collected from a living body and drain the tissue to reduce the amount of water contained in the tissue in order to produce a sheet-shaped cell culture. The collected tissue processed by the collected tissue shredding assist device 10 of this embodiment is skeletal muscle tissue. However, the collected tissue is not limited to skeletal muscle tissue and may be other tissues such as muscle tissue, adipose tissue, skin tissue, cartilage tissue, tendon tissue, ligament tissue, interstitial parenchyma, brain tissue, circulatory system tissue, digestive system tissue, metabolic system tissue, lymphatic system tissue, and bone marrow tissue.

[0019] 1, the collected tissue mincing assist device 10 of this embodiment includes a mounting body 20, a plurality of protrusions 30 arranged opposite the mounting body 20 and extending in a direction toward the mounting body 20, a disk-shaped support 40 supporting the protrusions 30, a pressing body 50 arranged between the mounting body 20 and the support 40, and a handle portion 60 connected to the protrusions 30 via the support 40. A disk-shaped lid portion 80 is provided at the middle position of the handle portion 60.

[0020] The mounting body 20 is formed in a thin disk shape and has a plurality of lower holes 22 penetrating in the thickness direction. The lower holes 22 are arranged at positions corresponding to the protrusions 30 so that they can be inserted therethrough. The mounting body 20 is fixed to a shaft 70 extending toward the handle 60.

[0021] The pressing body 50 is a thin disk-like shape roughly the same as the mounting body 20, and has a plurality of intermediate holes 52 that penetrate in the thickness direction. The intermediate holes 52 are formed at positions corresponding to the protrusions 30 so that they can be inserted therethrough. The pressing body 50 is movable along the length of the shaft portion 70. The shaft portion 70 is provided with a pressing body biasing portion 72 between the mounting body 20 and the pressing body 50, and when the pressing body 50 moves in a direction approaching the mounting body 20, the pressing body biasing portion 72 biases the pressing body in a direction away from the mounting body 20.

[0022] The protrusion 30 is a long member extending from the lower surface of the support 40 toward the mounting body 20. As shown in FIG. 2(a), the protrusion 30 has a semicircular cross section and a sharp blade surface 31 at its tip. The blade surface 31 enables the protrusion 30 to pierce and penetrate the collected tissue 210. Furthermore, because the protrusion 30 has a semicircular cross section, the surface area of ​​the hole formed in the collected tissue 210 can be increased. The protrusion 30 is thin enough not to cut through the fibrous fibers that make up skeletal muscle.

[0023] The protrusion 32 may have a V-shaped cross section as shown in FIG. 2(b). The protrusion 32 has a blade surface 33 at its tip. The protrusion 34 may have a V-shaped cross section with the tip protruding downward as shown in FIG. 2(c). The protrusion 36 may be plate-shaped with a sharp tip and multiple steps 36a on both sides as shown in FIG. 2(d). All of these can increase the surface area of ​​the hole formed in the collected tissue 210.

[0024] The support 40 that supports the protrusion 30 is formed in a disk shape and is fixed to the lower end of the handle portion 60. The support 40 is movable along the length of the shaft portion 70.

[0025] The handle portion 60 has an operating portion 62 that presses the support body 40 that supports the protrusion portion 36 toward the mounting body 20 , and a long portion 64 that connects the operating portion 62 and the support body 40 .

[0026] As shown in FIG. 3 , the collected tissue slicing assist device 10 can be stored in a centrifuge tube 200. The centrifuge tube 200 has a constant-diameter portion 201, which has the same inner diameter from the top to the bottom, and a tapered portion 202, which has a diameter that decreases toward the bottom. The mounting body 20, pressing body 50, and support body 40 of the collected tissue slicing assist device 10 have the same outer diameter that is close to the inner diameter of the constant-diameter portion 201. The lid portion 80 has an outer diameter that is larger than the outer diameter of the constant-diameter portion 201. Therefore, the mounting body 20 is engaged near the upper end of the tapered portion 202. The lid portion 80 can be positioned to close the centrifuge tube 200. When the collected tissue slicing assist device 10 is stored in the centrifuge tube 200 and the handle portion 60 is not pressed, a space is formed between the mounting body 20 and the pressing body 50, and the collected tissue 210 can be placed in this space. In this state, the tip of the protrusion 30 is located above the pressing body 50 and does not enter the space between the mounting body 20 and the pressing body 50 .

[0027] As shown in Fig. 4, the harvested tissue 210 can be placed on the mount 20. The handle portion 60 has a handle lumen 61 formed along the length of the long portion 64, and the upper end of the shaft portion 70 is located within the handle lumen 61. The upper end of the shaft portion 70 has an opposing surface portion 73 with an enlarged diameter, and a handle biasing portion 74 is provided between the opposing surface portion 73 and the upper surface of the handle lumen 61. When the handle portion 50 is pressed down by the handle biasing portion 74, it biases the handle portion 50 in a direction returning it to its original position.

[0028] When the operator operates the operating portion 62 of the handle portion 60 so as to press down, the handle portion 60 and the support body 40 fixed to the handle portion 60 move in a direction approaching the mounting body 20, as shown in FIG. 5 . Because the protrusion portion 30 is supported by the support body 40, it moves in a direction approaching the mounting body 20 in conjunction with the operation of the handle portion 60. The protrusion portion 30 penetrates the intermediate hole portion 52 of the pressing body 50 and enters the space between the mounting body 20 and the pressing body 50. The protrusion portion 30 then pierces the collected tissue 210 placed on the mounting body 20. The support body 40 moves toward the mounting body 20 and comes into contact with the pressing body 50.

[0029] When the operator further presses down the operating part 62 of the handle part 60 from the state shown in Fig. 5, the support body 40 moves even closer to the mounting body 20, pressing the pressing body 50 down toward the mounting body 20, as shown in Fig. 6. This reduces the distance between the mounting body 20 and the pressing body 50, causing the collected tissue 210 to be sandwiched between the mounting body 20 and the pressing body 50, squeezing out moisture. Furthermore, the protrusion 30 penetrates the lower hole 22 of the mounting body 20, with its tip positioned below the mounting body 20. This allows the protrusion 30 to form a through-hole in the collected tissue 210.

[0030] When the operator releases the handle portion 60, the handle portion 60 is moved by the handle biasing portion 74 in a direction away from the mounting body 20 relative to the shaft portion 70, and returns to the original position shown in Fig. 4. Also, the pressing body 50 is moved by the pressing body biasing portion 72 in a direction away from the mounting body 20 relative to the shaft portion 70, and returns to the original position shown in Fig. 4. The operator repeats the operation of pressing down the handle portion 60 and releasing his / her hand several times.

[0031] In this way, by pressing the handle portion 60, the protrusions 30 form through-holes in the collected tissue 210, while the collected tissue 210 is sandwiched between the mounting body 20 and the pressing body 50, allowing the collected tissue 210 to be drained. With through-holes formed in the collected tissue 210 and the water drained away to reduce the amount of moisture, the enzyme solution can more easily permeate the collected tissue 210, improving the efficiency of the enzyme reaction. Furthermore, the increased contact area of ​​the collected tissue 210 with the enzyme solution allows the chopping process to be carried out more efficiently, thereby shortening the time required for the chopping process.

[0032] After repeatedly operating the handle portion 60 several times, the operator can remove the collected tissue shredding assist device 10 from the centrifuge tube 200, thereby forming a through-hole and removing the drained collected tissue 210.

[0033] Next, a collected tissue mincing assist device 100 according to a first modified example will be described. As shown in Fig. 7, the collected tissue mincing assist device 100 has a support body 110 provided with a plurality of protrusions 30 and a needle 135. Like the protrusions 30, the needle 135 faces the mounting body 20 and extends in a direction toward the mounting body 20. The needle 135 has an inner cavity (not shown) that opens at the tip, and is shorter in length than the protrusions 30. The needle 135 is arranged on the support body 110 so as to be aligned with the protrusions 30.

[0034] The support 110 has a liquid storage section 112 in the form of a space capable of storing a liquid therein. The liquid storage section 112 is in communication with the inner cavity of the needle section 135. The liquid stored in the liquid storage section 112 is an enzyme solution. A pushing section 114 is disposed in the liquid storage section 112 for pushing the liquid therein into the inner cavity of the needle section 135. The pushing section 114 is connected to a second handle section 120 that is inserted into the handle section 60. The second handle section 120 has a second operating section 122 that can be operated to be pressed down relative to the handle section 60, and a second long section 123 that is inserted into the handle section 60 and connects the second operating section 122 and the pushing section 114.

[0035] As shown in FIG. 8 , when the handle portion 60 is pressed down, the second handle portion 120 can move downward together with the handle portion 60. When the handle portion 60 is pressed down, the protrusion 30 supported by the support 110 penetrates the mounting body 20, and the needle portion 135, which is shorter than the protrusion 30, is punctured into the collected tissue 210. From this state, when the second handle portion 120 is pressed down relative to the handle portion 60 as shown in FIG. 9 , the pusher 114 connected to the second handle portion 120 is pressed down, and the enzyme solution in the liquid reservoir 112 is injected from the needle portion 135 into the collected tissue 210. In this way, the collected tissue slicing assist device 100 can inject the enzyme solution in addition to forming a through hole in the collected tissue 210 and draining it. Therefore, the collected tissue slicing assist device 100 can efficiently proceed with the treatment with the enzyme solution before the slicing step. Furthermore, since the enzyme solution is injected directly into the collected tissue 210 by the needle portion 135, the enzyme solution can function effectively.

[0036] When the operator releases the handle portion 60 from the state in which the handle portion 60 is pressed down, the handle portion 60 is returned to its original position by the handle biasing portion 74, and the pressing body 50 is returned to its original position by the pressing body biasing portion 72. After injecting the enzyme solution into the collected tissue 210, the operator repeats the operation of the handle portion 60 several times, and then removes the collected tissue shredding assist device 100 from the centrifuge tube 200, forms a through-hole, and removes the collected tissue 210 that has been drained.

[0037] Next, a collected tissue collec- tion assist device 150 according to a second modified example will be described. As shown in Fig. 10, the collected tissue collec- tion assist device 150 has a handle portion 170 having an injection lumen 171 along the length and a port portion 172 to which an injection device such as a syringe can be connected for injecting liquid into the injection lumen. The injection lumen 171 communicates with a communication portion 162 provided in the support 160. The communication portion 162 communicates with the inner cavity of a needle portion 185 extending from the support 160 toward the mounting body 20. Therefore, liquid injected from the port portion 172 of the handle portion 170 can pass through the injection lumen 171 and the communication portion 162, and then from the needle portion 185 into the collected tissue 210.

[0038] In the collected tissue mincing assist device 150, similarly to the collected tissue mincing assist device 100, by operating the handle portion 170 so as to press down, the protrusion portion 30 and the needle portion 185 supported by the support body 160 move closer to the mounting body 20, the protrusion portion 30 penetrates the collected tissue 210, and the needle portion 185 punctures the collected tissue 210. In addition, the collected tissue 210 is sandwiched between the pressing body 50 and the mounting body 20. In this state, by injecting an enzyme solution from the port portion 172, the enzyme solution can be injected from the needle portion 185 into the collected tissue 210.

[0039] When the operator releases the handle portion 170 from the state in which the handle portion 170 is pressed down, the handle portion 170 is returned to its original position by the handle biasing portion 74, and the pressing body 50 is returned to its original position by the pressing body biasing portion 72. After injecting the enzyme solution into the collected tissue 210, the operator repeats the operation of the handle portion 60 several times, and then removes the collected tissue shredding assist device 150 from the centrifuge tube 200, forms a through-hole, and removes the collected tissue 210 that has been drained.

[0040] Next, a collected tissue shredding assist device 10 according to a third modified example will be described. As shown in Fig. 11(a), the collected tissue shredding assist device 10 has a shaft portion 70 fixed to a mounting body 20 on which the collected tissue 210 is placed, and the upper end of the shaft portion 70 is located in an inner cavity 76 of an intermediate shaft portion 75 fixed to a pressing body 50. A pressing body biasing portion 77 is disposed in the inner cavity 76 of the intermediate shaft portion 75. The upper end of the intermediate shaft portion 75 is disposed within a handle inner cavity 61 of a handle portion 60. A handle biasing portion 74 is disposed in the handle inner cavity 61.

[0041] As shown in FIG. 11( b), by operating the handle portion 60 so as to press down, the support 40 supporting the protrusion 30 approaches the mounting body 20, and the pressing body 50 is also pressed down by the support 40, causing the protrusion 30 to puncture the collected tissue 210 and sandwiching the collected tissue 210 between the pressing body 50 and the mounting body 20. At this time, the handle biasing portion 74 and the pressing body biasing portion 77 are both compressed, biasing the handle portion 60 and the pressing body 50 in directions returning them to their original positions. Therefore, when the operator releases the handle portion 60, the handle portion 60 and the pressing body 50 return to their original positions. The pressing body biasing portion 77 is disposed in the inner cavity 76 of the intermediate shaft portion 75 and is not exposed to the space in which the collected tissue 210 is disposed, so that the pressing body biasing portion 77 does not come into contact with the collected tissue 210.

[0042] In this embodiment, as shown in Fig. 7, the protrusion 30 and the needle 135 extend from different positions on the support 110 toward the mounting body 20, but as shown in Fig. 12, the protrusion 30 and the needle 135 may be arranged coaxially. In this case, the protrusion may have a shape as shown in Fig. 2(b) or 2(c).

[0043] As described above, the (1) collected tissue slicing assist device 10 according to this embodiment includes the mounting body 20 on which the collected tissue 210 is placed, the plurality of protrusions 30 arranged opposite the mounting body 20 and extending in the direction toward the mounting body 20, the pressing body 50 arranged between the mounting body 20 and the protrusions 30, having intermediate holes 52 through which the protrusions 30 are inserted and movable to change the distance from the mounting body 20, and the handle 60 to which the protrusions 30 are connected and which moves the protrusions 30 toward the mounting body 20. The collected tissue slicing assist device 10 configured in this manner can drain the collected tissue 210 by operating the handle 60 to puncture the plurality of protrusions 30 into the collected tissue 210 placed on the mounting body 20 and sandwiching the collected tissue 210 between the pressing body 50 and the mounting body 20. Therefore, the collected tissue pulverization assist device 10 can reduce the moisture content of the collected tissue 210 while forming multiple holes in the collected tissue 210, making it possible to efficiently add an enzyme solution to the collected tissue 210.

[0044] (2) In the collected tissue slicing assist device 10 described above in (1), the mounting body 20 may have a lower hole 22 through which the protrusion 30 is inserted. This allows the protrusion 30 of the collected tissue slicing assist device 10 to penetrate the mounting body 20, and therefore the protrusion 30 can penetrate the collected tissue 210 to form a through-hole, allowing the enzyme solution to be added to the collected tissue 210 more efficiently.

[0045] (3) The collected tissue slicing assist device 10 of (1) or (2) above may include a shaft portion 70 to which the mounting body 20 is fixed and which extends toward the handle portion 60, and the handle portion 60 may have a handle biasing portion 74 between the shaft portion 70 and the mounting body 20 that biases the shaft portion 70 in a direction away from the mounting body 20. This allows the protrusions 30 that have punctured the collected tissue 210 to return to their original positions when the handle portion 60 is released.

[0046] (4) In the collected tissue slicing assist device 10 described above in (3), the shaft portion 70 may have a pressing body biasing portion 72 that biases the pressing body 50 in a direction away from the mounting body 20. This allows the collected tissue slicing assist device 10 to return the pressing body 50, which holds the collected tissue 210, to its original position when the handle portion 60 is released.

[0047] (5) In the collected tissue collec- tion assist device 10 of any of the above (1) to (4), the protrusion 30 may have a cutting edge 37 at its tip. This allows the collected tissue collec- tion assist device 10 to reduce the force required to puncture the collected tissue 210 with the protrusion 30 to form a hole.

[0048] (6) The collected tissue mincing assist device 10 of any of (1) to (5) above is provided with a needle part 135 that is disposed opposite the mounting body 20 and extends in a direction toward the mounting body 20, and the needle part 135 has an inner cavity that opens at the tip and may have a length shorter than the protrusion part 30. This allows the collected tissue mincing assist device 10 to puncture the collected tissue 210 with the needle part 135 and directly inject a liquid such as an enzyme solution.

[0049] (7) In the collected tissue mincing assist device 10 of any of (1) to (6) above, the handle portion 60 may have an injection lumen 171 that communicates with the inner cavity of the needle portion 135, and a port portion 172 to which an injection device that injects liquid into the injection lumen 171 can be connected. This allows the collected tissue mincing assist device 10 to directly inject a liquid such as an enzyme solution into the collected tissue 210 via the needle portion 135 from an injection device connected to the port portion 172.

[0050] (8) The collected tissue slicing assist device 10 of any of (1) to (6) above may further include a liquid reservoir 112 that communicates with the lumen of the needle 135, and a second handle portion 120 that is inserted through the handle portion 60 and connected to a pusher 114 that delivers the liquid in the liquid reservoir 112 to the lumen of the needle 135. In this way, the collected tissue slicing assist device 10 can inject the liquid in the liquid reservoir 112 directly into the collected tissue 210 via the needle 135 by operating the second handle portion 120 while operating the handle portion 60 to insert the needle 135 into the collected tissue 210.

[0051] It should be noted that the present invention is not limited to the above-described embodiment, and various modifications can be made by those skilled in the art within the technical spirit of the present invention. In the above-described embodiment, the protrusions 30 penetrate the mount 20, and therefore the mount 20 is formed with the downward holes 22. However, the protrusions 30 may be long enough not to penetrate the mount 20, in which case the mount 20 does not need to have the downward holes 22. However, the mount 20 has at least holes or gaps that allow the water generated by squeezing the collected tissue 210 to pass toward the bottom of the centrifuge tube 200.

[0052] The mounting body 20 and the pressing body 50 may be formed of a mesh-like material, which allows the protrusions 30 and the needles 135 to penetrate the mounting body 20 and the pressing body 50. [Explanation of symbols]

[0053] 10. Collected tissue mincing assist device 20 Mounting body 22 Lower hole 30 Protrusion 40 Support 50 Pressurizing body 52 Intermediate hole 60 Handle 62 Operation section 64 Long section 70 Shaft 72 Pressing body biasing part 80 Lid 200 centrifuge tube 201 Same diameter part 202 Details First 210 Take the organization

Claims

1. a mounting body on which the collected tissue is mounted; a plurality of protrusions disposed opposite the mounting body and extending in a direction toward the mounting body; a pressing body disposed between the mounting body and the protrusion, the pressing body having an intermediate hole through which the protrusion is inserted, and movable so as to change the distance between the mounting body and the pressing body; a handle portion to which the protrusion portion is connected and which moves the protrusion portion toward the holder.

2. 2. The tissue harvesting and slicing assist device according to claim 1, wherein the mounting body has a lower hole through which the protrusion is inserted.

3. a shaft portion to which the mounting body is fixed and which extends toward the handle portion; 3. The tissue harvesting and slicing assist device according to claim 1, wherein the handle portion has a handle biasing portion between the handle portion and the shaft portion for biasing the handle portion in a direction away from the holder.

4. The medical device according to claim 3 , wherein the shaft portion has a pressing body biasing portion that biases the pressing body in a direction away from the mounting body.

5. 3. The tissue harvesting assist device according to claim 1, wherein the protrusion has a cutting edge at its tip.

6. a needle portion disposed opposite the mounting body and extending in a direction toward the mounting body; 3. The tissue harvesting and slicing assist device according to claim 1, wherein the needle portion has an inner cavity that opens at the tip end and has a length shorter than that of the protrusion portion.

7. 7. The tissue harvesting and pulverizing assist device according to claim 6, wherein the handle portion has an injection lumen communicating with the inner cavity of the needle portion, and a port portion to which an injection device for injecting a liquid into the injection lumen can be connected.

8. a fluid reservoir communicating with the lumen of the needle; 7. The tissue harvesting and slicing assist device according to claim 6, further comprising: a second handle portion connected to a pusher portion that is inserted through the handle portion and that delivers the liquid from the liquid reservoir portion to the lumen of the needle portion.

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