Method for producing fixed tissue from tissue having joint part and fixation kit for tissue having joint part

Abstract

To provide a method for producing a fixed tissue from a tissue having a joint part to reduce deformation of the joint part.SOLUTION: The method for producing a fixed tissue from a tissue having a joint part according to one embodiment of the present invention includes steps 1-3. The step 1 is a step of surrounding the tissue with a liquid-permeable material. The step 2 is a step of restricting deformation of the joint part by providing a restraining part to the liquid-permeable material. The step 3 is a step of immersing the tissue surrounded by the liquid-permeable material in a fixing solution.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a method for producing fixed tissue from tissue having an articular part and a kit for fixing tissue having an articular part. [Background technology]

[0002] In the fields of medicine and life science, when preparing samples such as pathological specimens from biological tissues, fixation is usually performed using formalin or the like to prevent tissue decay due to autolysis. During fixation, it is preferable that the external structure of the tissue does not deform before and after the tissue hardens. Therefore, various techniques have been proposed to prevent tissue deformation during fixation (see, for example, Patent Document 1). [Prior art documents] [Patent documents]

[0003] [Patent Document 1] Patent Publication No. 2009-092617 Summary of the Invention [Problem to be solved by the invention]

[0004] The manner in which tissues are deformed during fixation varies depending on the type of tissue being fixed. For example, when fixing tissues that include joints, the positional relationship of bones via the joints may change before and after the tissue is hardened. It is difficult to prevent such deformation with the technology disclosed in Patent Document 1.

[0005] An object of one aspect of the present invention is to provide a manufacturing method that can reduce deformation of a joint when manufacturing fixed tissue from tissue having a joint. [Means for solving the problem]

[0006] The present invention includes the following aspects. <1> A method for producing fixed tissue from tissue having an articulation, comprising the steps of: Step 1: Surrounding the tissue with a liquid-permeable material; Step 2: providing a restraint on the liquid-permeable material to limit deformation of the joint; Step 3: Immersing the tissue surrounded by the liquid-permeable material in a fixative. <2> The liquid-permeable material is a soft material. <1> The manufacturing method described in <3> The restraining portion is provided by disposing a restraining member or fusing a portion of the liquid-permeable material. <1> or <2> The manufacturing method described in <4> At least a part of the restraining portion also functions as an attachment portion for an identification tag for identifying the tissue. <1> ~ <3> 1. The manufacturing method according to any one of the preceding claims. <5> At least a part of the restraining portion also functions as a sealing portion to prevent the liquid-permeable material from unravelling. <1> ~ <4> 1. The manufacturing method according to any one of the preceding claims. <6> 1. A fixation kit for tissue having an articulation, comprising: a liquid permeable material for surrounding said tissue; A restraining member for limiting deformation of the joint. <7> Further comprising an identification tag for identifying the tissue. <6> The kit according to claim 1. [Effects of the Invention]

[0007] According to one aspect of the present invention, there is provided a manufacturing method that can reduce deformation of the joint when manufacturing fixed tissue from tissue having a joint. [Brief explanation of the drawings]

[0008] [Figure 1]1 is a flow chart showing a method for manufacturing a fixed tissue according to one embodiment of the present invention. [Figure 2] FIG. 2 is a schematic diagram showing the state of S1 (step 1) in the flow of FIG. [Figure 3] FIG. 2 is a schematic diagram showing the state of S2 (step 2) in the flow of FIG. [Figure 4] 10 is a schematic diagram illustrating an example of an embodiment in which a part of the restricting portion functions as a sealing portion. FIG. [Figure 5] 10A and 10B are schematic diagrams illustrating another example of an embodiment in which a part of the restricting portion functions as a sealing portion. [Figure 6] 10 is a schematic diagram showing an example of an embodiment in which a part of a restraining portion functions as an attachment portion for an identification tag. FIG. DETAILED DESCRIPTION OF THE INVENTION

[0009] However, the present invention is not limited to the following embodiments and various modifications may be made within the scope of the claims. Embodiments that combine technical means described in different embodiments are also included in the technical scope of the present invention.

[0010] Unless otherwise specified in this specification, "A to B" representing a numerical range means "greater than or equal to A and less than or equal to B." Unless otherwise specified in this specification, elements with the same reference numerals have the same or similar functions.

[0011] [Embodiment 1] A method for producing a fixed tissue according to one embodiment of the present invention will be described with reference to Figures 1 to 3. This method includes three steps: S1 (step 1), S2 (step 2), and S3 (step 3). Each step will be described in detail below.

[0012] [S1 (Process 1)] In S1 (step 1), the tissue 1 is surrounded by a liquid-permeable material 10 (see FIG. 2). In the example of FIG. 2, the tissue 1 is placed in a bag made of a mesh material through an opening P, thereby surrounding the tissue 1 with the liquid-permeable material 10.

[0013] The tissue 1 is a tissue having a joint 5. In the example of Fig. 2, the tissue 1 has only one joint 5. However, the tissue 1 may have two or more joints 5.

[0014] The joint 5 is a site where two or more bones are connected via tendons, ligaments, cartilage, etc. The relative positions of the two or more bones can change at the joint 5. Therefore, the joint 5 in this specification includes not only movable joints but also hemijoints, but does not include immovable joints. The joint 5 can be a uniaxial joint (such as a hinge joint, a spiral joint, or a pivot joint), a biaxial joint (such as a condylar joint, an ellipsoid joint, a saddle joint, or a plane joint), or a polyaxial joint (such as a ball-and-socket joint or a socket joint). In one embodiment, the joint 5 is a joint capable of flexion and extension.

[0015] The liquid-permeable material 10 is a material through which a liquid can flow. In one embodiment, the liquid-permeable material is a container having one or more storage compartments. The liquid-permeable material 10 may be made of a liquid-permeable material such as a mesh material. Alternatively, the liquid-permeable material 10 may be made of a liquid-impermeable material such as a plastic film, and may have openings through which a liquid can flow.

[0016] In one embodiment, the liquid-permeable material 10 is a soft material. A soft material refers to a material that is easily deformed when an external force is applied. Examples of soft materials include bags made of mesh material and bags made of plastic film. By using a soft material for the liquid-permeable material 10, space saving is promoted. For example, the liquid-permeable material 10 can be folded for storage. Alternatively, when immersed in a fixing solution, excess space is reduced by water pressure.

[0017] [S2 (Process 2)] In S2 (step 2), restraining portions 15 are provided on the liquid-permeable material 10 (see FIG. 3). This restricts deformation of the joints 5 included in the tissue 1. In the example of FIG. 3, restraining portions 15 are provided in four locations, restricting deformation (such as flexion and extension) of the joints 5. The number of restraining portions 15 is not particularly limited as long as it is sufficient to restrict deformation of the joints 5.

[0018] The restraint 15 may be provided by placing a restraining member such as a stapler or adhesive, or alternatively, the restraint 15 may be provided by fusing portions of the liquid permeable material 10 together.

[0019] When the joint 5 has multiple movement modes (for example, when the joint 5 can perform flexion-extension movement and abduction-adduction movement), the restricting unit 15 only needs to restrict at least one of the movement modes. In one embodiment, the restricting unit 15 restricts deformation of the joint 5 associated with flexion-extension movement.

[0020] The restraint 15 does not need to completely restrict the deformation of the joint 5. In one embodiment, the deformation of the joint 5 is restricted to 70% or less, 50% or less, 30% or less, or 10% or less of the maximum range of motion.

[0021] [S3 (Process 3)] In S3 (step 3), the tissue 1 surrounded by the liquid-permeable material 10 is immersed in a fixative. This allows the fixative to flow through the liquid-permeable material 10, and the tissue 1 comes into contact with the fixative while the deformation of the joint 5 is limited. Therefore, the tissue 1 is fixed while the deformation of the joint 5 is limited. Fixing the tissue 1 in this state can reduce changes in the positional relationship of the bones via the joint 5 before and after the tissue 1 hardens.

[0022] The fixed tissue obtained by the above-described production method can be used for any purpose, for example, by further processing such as embedding, sectioning, and staining to prepare a tissue specimen.

[0023] [Embodiment 2] The restraining portion 15 may also function as a sealing portion to prevent the enclosing by the liquid-permeable material 10 from coming undone. Such a configuration can prevent the tissue 1 from falling off the liquid-permeable material 10. Furthermore, if the restraining portion 15 that also functions as a sealing portion and the restraining portion 15 that does not also function as a sealing portion are the same, there is no need to separately prepare a special member.

[0024] In the example shown in Fig. 4, after the tissue 1 is placed into the liquid-permeable material 10a (small bag-shaped container) through the opening P, the deformation of the joint 5 is restricted by providing the restraining parts 15. Here, of the five restraining parts 15, at least two of the restraining parts 15 that are close to the opening P of the bag also function as sealing parts to prevent the opening P of the bag from opening and unravelling the enclosure of the tissue 1.

[0025] [Variations] In the example shown in FIG. 5, a sheet-like liquid-permeable material 10b is used instead of a bag-like material. Specifically, one sheet of liquid-permeable material 10b is folded and the tissue 1 is sandwiched between the folded sheets to enclose the tissue 1 (upper panel). Then, a restraining portion 15 is provided to maintain the two layers of liquid-permeable material 10b stacked together (lower panel). In this case, the restraining portion 15 is provided at a position that can limit deformation of the joint portion 5 and prevent the tissue 1 from falling off. In the example shown in FIG. 5, the restraining portion 15 also functions as a sealing portion to prevent the tissue 1 from coming undone.

[0026] [Embodiment 3] The restraining portion 15 may also serve as an attachment portion for an identification tag 20 for identifying the tissue 1. Such a configuration makes it possible to identify multiple tissues 1. This embodiment is useful when multiple tissues 1 need to be fixed simultaneously and distinguished from one another, such as when preparing multiple pathological specimens at once.

[0027] 6, one of the restraining portions 15 not only limits the deformation of the joint portion 5 but also serves as an attachment portion for the identification tag 20. When the restraining portion 15 is provided by arranging a restraining member (such as a stapler or adhesive), the identification tag 20 is made of a material and has a shape that allows the restraining member to be used. When the restraining portion 15 is provided by fusing the liquid-permeable material 10, the identification tag 20 is made of a material and has a shape that allows it to be fusing together with the liquid-permeable material 10.

[0028] [Embodiment 4] An embodiment of the present invention also includes a fixation kit for tissue 1 having an articular portion 5. The fixation kit includes a liquid-permeable material 10 and a restraining member. When the restraining member is placed on the liquid-permeable material 10, it functions as a restraining portion 15. The fixation kit may further include an identification tag 20. The fixation kit may include a liquid-permeable material 10a and / or a liquid-permeable material 10b in addition to (or instead of) the liquid-permeable material 10.

[0029] The fixation kit may include one or more elements useful in the kit in addition to the liquid-permeable material 10, the restraining member, and the identification tag 20. An example of an element useful in the kit is a fixative. Other examples of elements useful in the kit include instructions describing how to use the kit (e.g., how to fix the tissue 1) and information (e.g., a URL) for obtaining the instructions. The elements included in the fixation kit may have one or more of any of the features described in embodiment 1. [Explanation of symbols]

[0030] 1 :Organization 5: Joints 10:Liquid permeable material 10a: Liquid permeable material 10b: Liquid permeable material 15: Stopping part S1: Process 1 S2: Process 2 S3: Process 3

Claims

1. A method for producing fixed tissue from tissue having an articular portion, comprising the steps of: Step 1: Surrounding the tissue with a liquid-permeable material; Step 2: providing a restraint on the liquid-permeable material to limit deformation of the joint; Step 3: Immersing the tissue surrounded by the liquid-permeable material in a fixative.

2. The liquid-permeable material is a soft material. The method of claim 1.

3. The restraining portion is provided by disposing a restraining member or fusing a portion of the liquid-permeable material. The method of claim 1.

4. At least a part of the restraining portion also functions as an attachment portion for an identification tag for identifying the tissue. The method of claim 1.

5. At least a part of the restraining portion also functions as a sealing portion to prevent the liquid-permeable material from unravelling. The method of claim 1.

6. 1. A fixation kit for tissue having an articulation, comprising: a liquid permeable material for surrounding said tissue; A restraining member for limiting deformation of the joint.

7. Further comprising an identification tag for identifying the tissue. The kit of claim 6.

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