Resin film, container, cell container, and method for culturing cells

Abstract

To provide a resin film that exhibits both high oxygen permeability and high toughness, in particular high elongation at break in a heat-sealed area.SOLUTION: This resin film includes a layer containing a 4-methyl-1-pentene copolymer with the content of structural units derived from 4-methyl-1-pentene of 90 mol% or more and 96 mol% or less and the content of structural units derived from an α-olefin having 6 to 20 carbon atoms (excluding 4-methyl-1-pentene) of 4 mol% or more and 10 mol% or less. The resin film has an oxygen permeability at 23°C of 3.0 L / (m2 day am) or more and 100.0 L / (m2 day am) or less.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to a resin film, a container, a cell container, and a method for culturing cells. [Background technology]

[0002] Known cell containers for propagating, growing, storing, and transporting cells include those in which an oxygen-permeable film is heat-sealed to create a sealed state, and a port member attached to a part of the film connects the inside and outside of the container (see, for example, Patent Document 1). Patent Document 1 describes that the cell container is made from a gas-permeable film such as linear low-density polyethylene.

[0003] Also known as cell containers are those configured by attaching a resin sheet or film to the bottom surface of a cylindrical substrate to form a container, or by attaching a resin sheet or film to the top surface of a well to form a sealed container (for example, Patent Document 2). Patent Document 2 states that the attached resin sheet or film is preferably made of a material with high oxygen permeability, such as polyethylene. [Prior art documents] [Patent documents]

[0004] [Patent Document 1] International Publication No. 2022 / 014436 [Patent Document 2] Japanese Patent Application Publication No. 2018-033318 Summary of the Invention [Problem to be solved by the invention]

[0005] The use of a resin film with high oxygen permeability for cell containers can improve cell culture efficiency. Furthermore, films using 4-methyl-1-pentene copolymers are expected to be suitable for use as resin films for cell containers due to their high oxygen permeability.

[0006] On the other hand, resin films for cell containers are sometimes required to have high toughness so as not to break under the weight of the liquid contained in the container. In particular, when the film is heat-sealed to form a container as described in Patent Document 1, the heat-sealed portion is required to have a large breaking elongation.

[0007] The present invention has been made in consideration of the above-mentioned problems of the conventional technology, and aims to provide a resin film that combines high oxygen permeability with high toughness, particularly high breaking elongation in the heat-sealed portion, a container and cell container made from said resin film, and a method for culturing cells using said cell container. [Means for solving the problem]

[0008] One aspect of the present invention for solving the above problems relates to the following resin films [1] to

[12] . [1] A layer containing a 4-methyl-1-pentene copolymer in which the content of structural units derived from 4-methyl-1-pentene is 90 mol % or more and 96 mol % or less, and the content of structural units derived from an α-olefin (excluding 4-methyl-1-pentene) having 6 to 20 carbon atoms is 4 mol % or more and 10 mol % or less, Oxygen permeability at 23°C is 3.0L / (m 2 ·day · am) or more 100.0L / (m 2 ·day·am) or less, Resin film. [2] The layer comprises a mixture of two or more 4-methyl-1-pentene copolymers having different melting points as measured by differential scanning calorimetry (DCS); [1] The resin film according to the present invention. [3] The layer contains a mixture of a 4-methyl-1-pentene copolymer having no melting point observed by differential scanning calorimetry (DCS) and a 4-methyl-1-pentene copolymer having a melting point observed by differential scanning calorimetry (DCS), [1] or [2]. The resin film according to [1] or [2]. [4] The layer is subjected to cross-fractional chromatography (CFC) using o-dichlorobenzene as an eluent, and the elution start temperature (the temperature at which the cumulative elution weight percentage becomes 0.5 weight percent) is set to [T S ], and the dissolution end temperature (the temperature at which the cumulative dissolution weight percentage reaches 99% by weight) is [T E ], the elution temperature T E ~T S The 4-methyl-1-pentene copolymer mixture has two or more elution peaks, which are peaks that appear on a differential elution curve obtained by differentiating a cumulative elution curve obtained in the range of The resin film according to any one of [1] to [3]. [5] Total light transmittance is 70% or more and 100% or less. The resin film according to any one of [1] to [4]. [6] Haze is between 0.1% and 10%. The resin film according to any one of [1] to [5]. [7] The thickness is 50 μm or more and 1 mm or less. The resin film according to any one of [1] to [6]. [8] A multilayer film, the ratio of the thickness of the layer containing the 4-methyl-1-pentene copolymer to the total thickness of the resin film is 50% or more and 90% or less; The resin film according to any one of [1] to [7]. [9] The ratio of the oxygen permeability coefficient to the carbon dioxide permeability coefficient (O2 / CO2) is 1 / 4 or more and 1 / 2.5 or less; The resin film according to any one of [1] to [8].

[10] A film having a layer containing the 4-methyl-1-pentene copolymer and a sealant layer, The resin film according to any one of [1] to [9].

[11] The heat seal strength after heat sealing the sealant layers together at 160 ° C is 10 N / 15 mm or more and 100 N / 15 mm or less,

[10] The resin film according to

[10] .

[12] The elongation at peeling after heat sealing the sealant layers together at 160°C is 100% or more and 500% or less.

[10] or

[11] . The resin film according to

[10] or

[11] .

[0009] Another aspect of the present invention for solving the above problems relates to the following containers

[13] to

[15] .

[13] A container formed into a bag shape by heat-sealing one or more sheets of the resin film according to any one of [1] to

[12] .

[14] A port member that communicates the inside and the outside of the container, The port member is bonded to the resin film by heat sealing.

[13] A container according to the present invention.

[15] Cellular vessels,

[13] or

[14] .

[0010] Another aspect of the present invention for solving the above problems relates to a cell container as described below in

[17] .

[17] A resin film according to any one of [1] to

[12] , a substrate having a through-hole or a recess, the resin film is in close contact with the top surface or bottom surface of the through hole or the top surface of the recess; cell container.

[0011] Another aspect of the present invention for solving the above problems relates to the cell culture methods

[16] and

[18] below.

[16]

[15] , comprising a step of culturing cells inside the container. Cell culture methods.

[18]

[17] , comprising a step of culturing cells inside the cell container. Cell culture method [Effects of the Invention]

[0012] According to the present invention, there are provided a resin film that combines high oxygen permeability and high toughness, particularly high breaking elongation in the heat-sealed portion, a container and cell container made from said resin film, and a method for culturing cells using said cell container. [Brief explanation of the drawings]

[0013] [Figure 1] FIG. 1 is a plan view showing the appearance of a cell container having a port member made from a resin film. [Figure 2] FIG. 2 is a partial cross-sectional view of the cell container shown in FIG. 1 taken along dashed line 1B-1B. [Figure 3] FIG. 3 is a perspective view of a culture vessel having a resin film. [Figure 4] 4A and 4B are cross-sectional views of a portion of the culture vessel shown in FIG. 3, showing another embodiment of the culture vessel. [Figure 5] 5A and 5B are bottom and top perspective views of another culture vessel having a resin film. [Figure 6] FIG. 6 is a cross-sectional view of a portion of the culture vessel. [Figure 7] FIG. 7 is a schematic diagram showing how the cell container sheet is peeled off to collect the nerve tissue. DETAILED DESCRIPTION OF THE INVENTION

[0014] 1. Resin film The first embodiment of the present invention relates to a resin film. The resin film may be a single layer or a multi-layer laminate.

[0015] (Layer composition) When the resin film is a multilayer laminate, it can be a laminate having a first outer layer and a second outer layer disposed on the outermost surfaces of both sides of the film and a core layer sandwiched therebetween. The resin film as a multilayer laminate may be a two-layer laminate having either the first outer layer or the second outer layer and the core layer, or may be a multi-layer laminate having layers other than these.

[0016] In this case, the first and second outer layers can be support layers that sandwich and hold the core layer from above and below. The second outer layer can also be a sealant layer that improves heat sealing properties. The first and second outer layers can also be layers that suppress blocking when a soft, highly oxygen-permeable core layer is layered on top of the resin film, thereby improving the anti-blocking properties of the resin film.

[0017] The thickness of outer layer 1 and outer layer 2 is preferably 5 μm or more and 300 μm or less, more preferably 5 μm or more and 200 μm or less, and even more preferably 5 μm or more and 150 μm or less. The thicker the outer layer, the more the heat-sealing property of the base film can be improved. Furthermore, the thinner the outer layer, the more the oxygen permeability of the resin film can be improved.

[0018] The thickness of the core layer is preferably 10 μm or more and 0.5 mm or less, more preferably 15 μm or more and 0.3 mm or less, and even more preferably 20 μm or more and 100 μm or less. The thicker the core layer, the more the flatness of the film can be improved. By appropriately thinning the thickness of the core layer, the oxygen permeability of the film can be improved.

[0019] (material) The resin film contains a 4-methyl-1-pentene copolymer in at least one of these layers, which can increase the oxygen permeability of the resin film.

[0020] In addition, the resin film preferably contains at least one thermoplastic resin or thermoplastic elastomer selected from the group consisting of ethylene-based (co)polymers, propylene-based (co)polymers, butene-based (co)polymers, and styrene-based elastomers in at least one of these layers. By using an appropriate combination of these thermoplastic resins or thermoplastic elastomers, the transparency, oxygen permeability, and heat seal strength of the resin film can be well balanced.

[0021] The resin film preferably contains 4-methyl-1-pentene-propylene copolymer in at least the outer layer 2 (sealant layer). This improves the heat seal strength and oxygen and carbon dioxide gas permeability of the resin film. The resin film also preferably contains a butene copolymer in at least the outer layer 2 (sealant layer). This improves the heat sealability and transparency of the resin film.

[0022] The resin film preferably contains a thermoplastic elastomer such as a polyolefin elastomer or a styrene elastomer in the core layer. Hydrogenated SEBS, SEPS, and SEEPS are preferably used as the styrene elastomer. Among these, polyolefin elastomers are preferred, and 4-methyl-1-pentene copolymers are more preferred, from the viewpoint of improving the flatness and oxygen permeability of the resin film. In this specification, the term "thermoplastic elastomer" refers to a polymer that exhibits fluidity when heated above its melting point (if crystalline) or above its glass transition point (if amorphous), while exhibiting rubber elasticity at room temperature.

[0023] For example, the resin film may be a multilayer film having outer layers containing a 4-methyl-1-pentene copolymer and a butene copolymer, and a core layer containing a 4-methyl-1-pentene copolymer.

[0024] The ethylene-based (co)polymer may be a homopolymer of ethylene or a copolymer of ethylene and an α-olefin having 3 to 20 carbon atoms. Of these, a copolymer of ethylene and an α-olefin having 3 to 20 carbon atoms is preferred. From the viewpoint of improving gas permeability and heat sealability, the α-olefin having 3 to 20 carbon atoms is preferably propylene, butene, hexene, or octene. The proportion of structural units derived from ethylene to all structural units of the ethylene-based (co)polymer is preferably 80 mol% to 95 mol%, more preferably 85 mol% to 95 mol%, and even more preferably 88 mol% to 93 mol%. The proportion of structural units derived from α-olefins having 3 to 20 carbon atoms to all structural units of the ethylene-based (co)polymer is preferably 5 mol% to 20 mol%, more preferably 5 mol% to 15 mol%, and even more preferably 7 mol% to 12 mol%.

[0025] The ethylene (co)polymer may also be a block polymer of ethylene and silicone.

[0026] The block copolymer may be a (polyethylene)-(silicone) diblock copolymer or a (polyethylene)-(silicone)-(polyethylene) terblock copolymer. The polyethylene may be an ethylene homopolymer or a copolymer of ethylene and an α-olefin having 3 to 20 carbon atoms, but is preferably an ethylene homopolymer. When the polyethylene is a copolymer, the molar ratio of ethylene to the other α-olefin (ethylene:other α-olefin) is preferably 81:19 to 99:1, more preferably 90:10 to 99:1.

[0027] The block copolymer can be obtained by reacting polyethylene having a vinyl group at one end with a silicon-containing compound in the presence of a catalyst, specifically by a method described in WO 2012 / 098865 or the like.

[0028] For example, the block copolymer may have the following structure:

[0029] A-CH2-CH2-Si(CH3)2-O-(Si(CH3)2-O) i -Si(CH3)2-CH2-CH2-A The two As independently represent polyethylene, and i represents an integer of 1 or greater. The polyethylene represented by each As preferably has a number average molecular weight (Mn) of 100 or greater and 500,000 or less, more preferably 500 or greater and 50,000 or less, and even more preferably 700 or greater and 10,000 or less. i is preferably 1 or greater and 1,000 or less, more preferably 1 or greater and 300 or less, and even more preferably 1 or greater and 50 or less.

[0030] The propylene-based (co)polymer may be a homopolymer of propylene, or a copolymer of propylene with ethylene or an α-olefin other than propylene. Of these, a copolymer of propylene with an α-olefin other than propylene is preferred. The α-olefin other than propylene is preferably ethylene or an α-olefin having from 4 to 20 carbon atoms, and from the viewpoint of improving gas permeability and heat sealability, ethylene, butene, hexene, and octene are preferred. The proportion of structural units derived from propylene to all structural units of the propylene-based (co)polymer is preferably 70 mol% to 95 mol%. The proportion of structural units derived from ethylene or an α-olefin other than propylene to all structural units of the propylene-based (co)polymer is preferably 5 mol% to 30 mol%.

[0031] The 4-methyl-1-pentene copolymer is a copolymer of 4-methyl-1-pentene and an α-olefin other than ethylene or 4-methyl-1-pentene. The 4-methyl-1-pentene copolymer is preferably a copolymer of 4-methyl-1-pentene and an α-olefin other than ethylene or 4-methyl-1-pentene, and more preferably a copolymer of 4-methyl-1-pentene and propylene. The proportion of structural units derived from 4-methyl-1-pentene to all structural units of the 4-methyl-1-pentene copolymer is preferably 45 mol% to 95 mol%, more preferably 70 mol% to 93 mol%, and even more preferably 75 mol% to 90 mol%. The proportion of structural units derived from ethylene or an α-olefin other than 4-methyl-1-pentene to all structural units of the 4-methyl-1-pentene copolymer is preferably 5 mol% to 55 mol%, more preferably 7 mol% to 30 mol%, and even more preferably 10 mol% to 25 mol%.

[0032] The butene copolymer is preferably a copolymer of butene and ethylene or an α-olefin other than butene, and is preferably a copolymer of 1-butene and ethylene, or a copolymer of 1-butene and propylene. The butene copolymer preferably contains structural units derived from 1-butene in a proportion of its total structural units of 90 mol% to 99 mol%, more preferably 93 mol% to 98 mol%, and even more preferably 94 mol% to 97 mol%. The butene copolymer preferably contains structural units derived from ethylene or an α-olefin other than butene in a proportion of its total structural units of 1 mol% to 10 mol%, more preferably 2 mol% to 7 mol%, and even more preferably 3 mol% to 6 mol%.

[0033] The resin film contains, in at least one layer, a 4-methyl-1-pentene copolymer (hereinafter simply referred to as "rubber-containing 4MP1") having a content of structural units derived from 4-methyl-1-pentene of 90 mol % to 96 mol % and a content of structural units derived from an α-olefin having 6 to 20 carbon atoms (excluding 4-methyl-1-pentene; hereinafter simply referred to as "other α-olefins") of 4 mol % to 10 mol %. The rubber-containing 4MP1 itself exhibits high elongation at break. Therefore, the use of the rubber-containing 4MP1 can increase the elongation at break of the resin film, particularly the elongation at break of the heat-sealed portion.

[0034] The other α-olefin is preferably a linear α-olefin. The other α-olefin is preferably an α-olefin having 10 to 18 carbon atoms. Examples of the other α-olefin include 1-hexene, 1-octene, 1-decene, 1-dodecene, 1-tetradecene, 1-hexadecene, 1-heptadecene, and 1-octadecene. Of these, 1-decene, 1-hexadecene, and 1-octadecene are preferred.

[0035] The rubber-containing 4MP1 may contain structural units derived from monomers other than these in an amount of less than 10 mol % relative to the total number of moles.

[0036] The 4MP1 rubber may be a mixture of multiple 4-methyl-1-pentene copolymers having different compositions or properties.

[0037] For example, the rubber-containing 4MP1 can be a mixture of two or more 4-methyl-1-pentene copolymers with different melting points measured by differential scanning calorimetry (DCS), or a mixture of a 4-methyl-1-pentene copolymer with no melting point measured by DCS and a 4-methyl-1-pentene copolymer with a melting point measured by DCS. The former mixture and layers containing it exhibit two or more melting peaks in the endothermic curve measured by differential scanning calorimetry (DSC measurement device). Such mixtures have a wide compositional distribution and are therefore more likely to be compatible with other resins. As a result, they are less likely to suffer from loss of transparency or haze due to phase separation.

[0038] The rubber-containing 4MP1 preferably has an endothermic peak observed in the endothermic curve obtained by DSC in the range of 180°C to 250°C, more preferably in the range of 200°C to 245°C, and even more preferably in the range of 220°C to 245°C. Furthermore, the rubber-containing 4MP1 preferably has another endothermic peak observed in the endothermic curve obtained by DSC below 180°C, more preferably in the range of 100°C to 175°C, and even more preferably in the range of 100°C to 170°C. 4-methyl-1-pentene copolymers having endothermic peaks in these ranges have high compatibility and similar refractive indices. Therefore, when the 4-methyl-1-pentene copolymer is mixed, a decrease in transparency or haze due to phase separation is unlikely to occur.

[0039] In addition, rubber-containing 4MP1 was analyzed by cross-fractional chromatography (CFC) using o-dichlorobenzene as the eluent, and the elution start temperature (the temperature at which the cumulative elution weight percentage reaches 0.5 weight percent) was [T S ], and the dissolution end temperature (the temperature at which the cumulative dissolution weight percentage reaches 99% by weight) is [T E ], the elution temperature T E ~T S It is preferable that the 4-methyl-1-pentene copolymer mixture contains two or more elution peaks that appear on a differential elution curve obtained by differentiating a cumulative elution curve obtained in the range.

[0040] In these 4-methyl-1-pentene copolymer mixtures, components with high melting points or high elution peak temperatures are thought to impart appropriate hardness (rigidity) to the resin film and improve moldability, while components with low or no melting points or low elution peak temperatures are thought to improve the extensibility of the resin film.

[0041] The rubber-containing 4MP1 can be, for example, a mixture of 4-methyl-1-pentene copolymers as follows:

[0042] 4MP1-(A) A copolymer having a content of structural units derived from 4-methyl-1-pentene of 97.5 mol % or more but less than 100 mol %, and a content of structural units derived from other α-olefins of more than 0 mol % but not more than 2.5 mol %. 4MP1-(B) A copolymer having a content of structural units derived from 4-methyl-1-pentene of 91.0 mol% or more but less than 97.5 mol%, and a content of structural units derived from other α-olefins of more than 2.5 mol% but not more than 9.0 mol%. 4MP1-(C) A copolymer having a content of structural units derived from 4-methyl-1-pentene of less than 91.0 mol % and a content of structural units derived from other α-olefins of more than 9 mol %.

[0043] 4MP1-(A) can impart appropriate hardness to the resin film and also improve the heat resistance and moldability.

[0044] 4MP1-(A) preferably contains 97.5 to 99.5 mol % of structural units derived from 4-methyl-1-pentene, more preferably 97.7 to 99.0 mol %, and preferably contains 0.5 to 2.5 mol %, more preferably 1.0 to 2.3 mol %, of structural units derived from other α-olefins.

[0045] The intrinsic viscosity [η] of 4MP1-(A) measured in decalin at 135°C is preferably 1.0 dl / g or more and 5.0 dl / g or less, more preferably 1.0 dl / g or more and 4.0 dl / g or less, and even more preferably 1.0 dl / g or more and 3.0 dl / g or less.

[0046] 4MP1-(A) preferably has a melting point (Tm) (endothermic peak) measured by differential scanning calorimetry (DSC) in the range of 180°C or higher and 250°C or lower, more preferably in the range of 200°C or higher and 245°C or lower, and even more preferably in the range of 220°C or higher and 245°C or lower.

[0047] 4MP1-(B) improves the elongation while maintaining the formability of the resin film.

[0048] 4MP1-(B) preferably contains 91.5 to 96.0 mol % of structural units derived from 4-methyl-1-pentene, more preferably 92.0 to 95.0 mol %, and preferably contains 4.0 to 8.5 mol %, more preferably 5.0 to 8.0 mol %, of structural units derived from other α-olefins.

[0049] The intrinsic viscosity [η] of 4MP1-(B) measured in decalin at 135°C is preferably 2.0 dl / g or more and 5.0 dl / g or less, more preferably 3.0 dl / g or more and 5.0 dl / g or less, and even more preferably 3.5 dl / g or more and 5.0 dl / g or less.

[0050] 4MP1-(B) preferably has a melting point (Tm) (endothermic peak) measured by differential scanning calorimetry (DSC) of less than 180°C, more preferably in the range of 100°C or higher and 175°C or lower, and even more preferably in the range of 100°C or higher and 170°C or lower.

[0051] 4MP1-(C) increases the elongation of the resin film.

[0052] 4MP1-(C) preferably has a content of structural units derived from 4-methyl-1-pentene of 50.0 mol% or more but less than 91.0 mol%, more preferably 70.0 mol% or more but 90.0 mol% or less, and a content of structural units derived from other α-olefins of preferably more than 9.0 mol% but 50.0 mol% or less, more preferably 10.0 mol% or more but 30.0 mol% or less.

[0053] The intrinsic viscosity [η] of 4MP1-(C) measured in decalin at 135°C is preferably 2.0 dl / g or more and 5.0 dl / g or less, more preferably 3.0 dl / g or more and 5.0 dl / g or less, and even more preferably 3.5 dl / g or more and 5.0 dl / g or less.

[0054] It is preferable that 4MP1-(C) does not exhibit a melting point (Tm) (endothermic peak) when measured by a differential scanning calorimeter (DSC).

[0055] 4MP1-(A) to 4MP1-(C) can all be obtained by polymerizing 4-meryl-1-pentene with other α-olefins in the presence of an olefin polymerization catalyst. The physical properties of each copolymer can be adjusted by adjusting the amount of hydrogen added during polymerization.

[0056] The content of 4MP1-(A) relative to the total mass of 4MP1-(A) to 4MP1-(C) is preferably 15% by mass or more and 40% by mass or less, more preferably 20% by mass or more and 40% by mass or less, and even more preferably 24% by mass or more and 36% by mass or less.

[0057] The content of 4MP1-(B) relative to the total mass of 4MP1-(A) to 4MP1-(C) is preferably 40% by mass or more and 80% by mass or less, more preferably 40% by mass or more and 70% by mass or less, and even more preferably 44% by mass or more and 66% by mass or less.

[0058] The content of 4MP1-(C) relative to the total mass of 4MP1-(A) to 4MP1-(C) is preferably 5% by mass or more and 30% by mass or less, more preferably 7% by mass or more and 25% by mass or less, and even more preferably 9% by mass or more and 25% by mass or less.

[0059] In the case of a multilayer film, the thickness of the layer containing rubber-containing 4MP1 is preferably 50% to 90% of the total thickness of the resin film, and more preferably 60% to 90%. The greater the proportion of the layer containing rubber-containing 4MP1, the greater the extensibility of the resin film.

[0060] The resin film preferably contains rubber-containing 4MP1 in the core layer, and preferably the core layer is made of only rubber-containing 4MP1. This allows the resin film to have more sufficient elongation. The resin film also preferably contains rubber-containing 4MP1 in the outer layer 1, and preferably the outer layer 1 contains rubber-containing 4MP1 and a butene copolymer. This allows the resin film to have more sufficient elongation, and also allows the resin film to have improved transparency and antiblocking properties.

[0061] (Physical properties of resin film) From the viewpoint of improving cell observation efficiency, the resin film preferably has high visible light transmittance. Specifically, the total light transmittance measured in accordance with JIS K 7361-1:1997 is preferably 70% to 100%, more preferably 80% to 100%, and even more preferably 90% to 100%. From the same viewpoint, the resin film preferably has a haze measured in accordance with JIS K 7136:2000 of 0.1% to 10%, more preferably 0.1% to 5%, and even more preferably 0.1% to 1%.

[0062] The resin film has an oxygen permeability of 3.0 L / (m at 23°C. 2 ·day · atm) or more 100.0L / (m 2·day·atm) or less, and 4.0L / (m 2 ·day · atm) or more 50.0L / (m 2 ·day·atm) or less is more preferable, and 5.0L / (m 2 ·day · atm) or more 30.0L / (m 2 It is more preferable that the resin film has an oxygen permeability coefficient of 0.3 L·mm / (m 2 ·24hr·atm) or more 10.0L·mm / (m 2 ·24hr·atm) or less, and 0.4Lmm / (m 2 ·24hr·atm) or more 5.0Lmm / (m 2 ·24hr·atm) or less is more preferable, and 0.5L·mm / (m 2 ·24hr·atm) or more 3.0Lmm / (m 2 It is more preferable that the oxygen permeability and oxygen permeability coefficient of the film are not more than 24 hr·atm. By increasing the oxygen permeability and oxygen permeability coefficient of the film, the efficiency of cell culture using the cell container can be improved.

[0063] In addition, the resin film has a carbon dioxide permeability of 10 L / (m at 23°C. 2 ·day · atm) or more 400L / (m 2 ·day·atm) or less, and 10L / (m 2 ·day · atm) or more 300L / (m 2 It is more preferable that the carbon dioxide permeability coefficient of the resin film at 23°C is 0.1 L·mm / (m 2 ·24hr·atm) or more 40.0L·mm / (m 2 ·24hr·atm) or less, and 0.1L·mm / (m 2 ·24hr·atm) or more 30.0L·mm / (m 2 The higher the carbon dioxide permeability and the carbon dioxide permeability coefficient are, the higher the cell culture efficiency of the cell container can be.

[0064] Furthermore, the ratio of the oxygen permeability coefficient to the carbon dioxide permeability coefficient (O2 / CO2) of the resin film is preferably 1 / 4 or more and 1 / 2.5 or less, and more preferably 1 / 3.6 or more and 1 / 2.5 or less. By allowing more oxygen to permeate than carbon dioxide, the efficiency of cell culture in the cell container can be improved. By allowing a certain amount of carbon dioxide permeation, it is easier to optimize the culture environment, for example by controlling the pH of the culture medium.

[0065] The oxygen permeability and carbon dioxide permeability were measured in accordance with JIS K 7126-1:2006 using a differential pressure gas permeability measuring device (manufactured by Toyo Seiki Seisakusho) at a test temperature of 23°C and a test relative humidity of 0%RH, with the film measuring area at 5cm. 2 When the oxygen permeability or carbon dioxide permeability is expected to be large, an aluminum mask is placed on the measurement sample in advance to reduce the actual permeation area to 5.0 cm. 2 It is more preferable to set the following.

[0066] The resin film has a moisture permeability of 10 g / (m) at 40°C and a relative humidity of 90%. 2 ·day) or more 100g / (m 2 ·day) or less, and 12g / (m 2 ·day) or more than 80g / (m 2 ·day) or less is more preferable, and 14g / (m 2 ·day) or more 60g / (m 2 By setting the moisture permeability in this range, the contents are less likely to lose moisture.

[0067] The moisture permeability is calculated under condition B (test temperature 40°C, test relative humidity 90% RH) in accordance with the isobaric method (cup type-gravimetric method) described in JIS Z 0208:2021.

[0068] Furthermore, the resin film preferably has high weld strength and high elongation at the time of peeling after the resin films are heat-sealed together (after the sealant layers, if any, are heat-sealed together). Specifically, the weld strength when the resin films are heat-sealed together is preferably 10 N / 15 mm or more and 100 N / 15 mm or less, more preferably 15 N / 15 mm or more and 100 N / 15 mm or less, and even more preferably 20 N / 15 mm or more and 100 N / 15 mm or less. Furthermore, the elongation at the time of peeling after the resin films are heat-sealed together is preferably 100% or more and 500% or less, more preferably 150% or more and 500% or less, and even more preferably 200% or more and 500% or less.

[0069] To measure the weld strength and peel elongation after heat-sealing resin films together, the resin film was cut with its MD as the machine direction, creating two rectangular heat-seal test pieces measuring 200 mm wide x 60 mm long. These two heat-seal test pieces were then stacked with their outer layers 2 facing each other and heat-sealed using a heat-sealing tester (Tester Sangyo Co., Ltd., Thermal Gradient Heat-Sealing Tester TP-701-G) under the following conditions: upper and lower temperatures (heat-sealing temperature) of 160°C, seal width of 10 mm, seal pressure of 0.1 MPa, and seal time of 2 seconds. The welded multilayer films were then heat-sealed so that the weld line was perpendicular to the MD of the multilayer film. The two welded multilayer films were then removed from the tester, and 15 mm-wide strips were cut out perpendicular to the weld line to serve as heat-sealing test pieces. Using a tensile testing machine (Orientec Co., Ltd., Tensilon universal material testing machine, model RTG-1250), the heat-sealed test piece was pulled in a direction parallel to the longitudinal direction of the test piece and peeled at a test temperature of 23°C, a chuck distance of 50 mm, and a pulling speed of 300 mm / min, and the maximum peel strength and the elongation at peeling relative to the chuck distance were measured, and these were taken as the heat-seal strength (unit: N / 15 mm) and the elongation at peeling (unit: %), respectively. The heat-seal strength and elongation at peeling were measured for 35 heat-sealed test pieces, and the average values ​​were calculated.

[0070] Furthermore, the resin film preferably has high weld strength to other resin components, such as the port component of a culture bag, and high elongation at the welded portion when peeled off. Specifically, the resin film preferably has a peel strength of 20 N / 15 mm to 100 N / 15 mm, more preferably 25 N / 15 mm to 100 N / 15 mm, and even more preferably 30 N / 15 mm to 100 N / 15 mm, as measured by a 180° peel test under the following measurement conditions. Furthermore, the elongation at the welded portion when peeled off is preferably 50% to 500%, more preferably 100% to 500%, and even more preferably 150% to 500%.

[0071] To measure the weld strength and peel elongation when heat-sealing a resin film to another resin component, a compression molding machine (Shindo Metal Industries, ASF-10, maximum clamping force 10 ton) and a cooling compression molding machine (Shindo Metal Industries, NSF-37, maximum clamping force 37 ton) were used to prepare a press sheet measuring 65 mm wide x 65 mm long x 1 mm thick from a copolymer containing 97.60 mol% 4-methyl-1-pentene-derived structural units, 1.44 mol% 1-hexadecene-derived structural units, and 0.96 mol% 1-octadecene-derived structural units. The heating temperature was 270°C, the preheating time was 10 minutes, the pressurization time was 3 minutes, and the cooling time was 5 minutes.

[0072] The outer layer 2 (sealant layer) of the resin film was then placed on the surface of the press sheet, and the pieces were heat-sealed using a heat seal tester (Tester Sangyo Co., Ltd., thermal gradient heat seal tester, model TP-701-G) under the following conditions: upper heat seal bar temperature (heat seal temperature) 240°C, lower heat seal bar temperature 23°C, seal width 10 mm, seal pressure 0.1 MPa, and seal time 2 seconds. The heat seal was performed so that the weld line was perpendicular to the MD direction of the multilayer film. The welded multilayer film and press sheet were then removed from the tester, and a 15 mm wide strip specimen was cut out perpendicular to the weld line to serve as the heat-sealed specimen. Using a tensile testing machine (Orientec Co., Ltd., Tenshin Universal Material Testing Machine, Model RTG-1250), the welded strip-shaped test pieces were pulled in a direction parallel to the longitudinal direction of the test pieces and peeled under conditions of a test temperature of 23°C, a chuck distance of 50 mm, and a tensile speed of 300 mm / min, and the peel strength and the maximum elongation at peeling relative to the chuck distance were measured and recorded as the peel strength (unit: N / 15 mm) and the elongation at peeling (unit: %), respectively. The peel strength and elongation at peeling were measured for three test pieces and the average values ​​were calculated.

[0073] In addition, the resin film has a sterility assurance level (SAL) of 10 for medical devices measured in accordance with BS EN556-1:2001. -6 It is preferable that:

[0074] The resin film preferably has a thickness of 50 μm to 1 mm, more preferably 50 μm to 300 μm, even more preferably 50 μm to 200 μm, and particularly preferably 70 μm to 200 μm. The greater the thickness, the stronger the resin film can be. The smaller the thickness, the higher the oxygen permeability of the resin film can be.

[0075] 2. Vessel, cell vessel, and method for culturing cells (Culture bag) The resin film can be made into a container by placing two films one on top of the other, or by folding one film and placing the ends on top of each other, and welding the ends to form a bag shape.

[0076] FIG. 1 is a plan view showing the appearance of a cell container having a port member made from a resin film, and FIG. 2 is a partial cross-sectional view of the cell container shown in FIG. 1 taken along the dashed line 1B-1B.

[0077] The cell container 100 refers to a container including a bag-like shape used for culturing cells by introducing a culture medium and cells therein. In this specification, culturing cells means multiplying, growing, or maintaining the cells in a living state.

[0078] The cell container 100 is formed by overlapping two resin films 112 and 114 and welding these films together around the entire periphery of the edge to form a sealed portion 116, thereby forming a substantially sealed bag portion 110. In a portion of the welded sealed portion 116, one or more port members 200 (three in this embodiment) are sandwiched between the resin films 112 and 114. The port members 200 are tubular members that communicate between the inside and outside of the bag portion 110, and are welded to and bonded to the resin films 112 and 114, respectively.

[0079] The resin film 112 and the resin film 114 may be a single layer or a multilayer, but a multilayer film having a sealant layer is preferred.

[0080] When welding is performed using the heat sealing method, the sealing temperature is set to a temperature above the melting point of the resin contained in the port member 200 and the film if the resin is a crystalline resin, or above the glass transition point if either of these resins is amorphous. The heat sealing conditions should be set taking into account the speed of heat transfer from the heat seal bar to the welding interface due to the film thickness, productivity, and heat seal strength. For example, the heat sealing temperature can be set to 100°C or higher and 290°C or lower, preferably 130°C or higher and 280°C or lower. The heat sealing pressure can be set to 0.1 MPa or higher and 1 MPa or lower, preferably 0.1 MPa or higher and 0.5 MPa or lower. The seal time can be set to 1 second or higher and 10 seconds or lower, preferably 1 second or higher and 5 seconds or lower.

[0081] When overlapping and arranging the resin films, the resin films may be shaped into a predetermined shape using methods such as vacuum forming, pressure forming, and vacuum pressure forming. In this case, the edges of the resin films must be overlapped. Instead of a heat-sealing method in which the overlapped films are sandwiched between hot plates, the resin films may be welded using impulse sealing, high-frequency welding, ultrasonic welding, laser welding, or other methods. For example, in the case of laser welding, the edges of the overlapped resin films are irradiated with laser light having a wavelength of 1.8 to 2 μm emitted from a Ho-YAG laser or Tm fiber laser, or laser light having a wavelength of 10.6 μm emitted from a carbon dioxide laser. This allows the resin films to directly absorb the energy of the laser light, welding the edges together.

[0082] Furthermore, the number of resin films used to fabricate the cell container 100 is not limited to two. A single resin film may be folded and arranged with its ends overlapping, and the ends may be heat-sealed to form a bag, or a cell container may be formed using three or more resin films.

[0083] Furthermore, after the cell container is produced, it can be sterilized before use. Sterilization is preferably performed by gamma ray sterilization, since the film material is resistant to gamma ray irradiation. The gamma ray irradiation dose is preferably 10 to 50 kGy. Furthermore, from the viewpoint of preventing contamination and maintaining sterility, the cell container is preferably used as a single-use item.

[0084] The above-described cell container 100 can be used for culturing various types of cells.

[0085] Specifically, an injection port for injecting medicines or the like and a tube for connecting to a Luer port are attached to each port member 200. Then, a culture solution containing cells (cell suspension) is introduced into the cell container 100 from the Luer port connected to the tube. Note that a culture solution not containing cells may be introduced into the cell container 100 first, and then the cell suspension may be introduced.

[0086] Thereafter, the cell container 100 is placed in an incubator to culture the cells. The conditions for culturing the cells are not particularly limited and may be selected depending on the cells to be cultured.

[0087] During the culture, a drug may be injected through the injection port, or a portion of the cells may be sampled to check the culture state. Also, some or all of the cells may be transferred to another cell container 100 via a tube attached to the port member.

[0088] Finally, the cultured cells are collected through a tube attached to the port member.

[0089] The cells targeted by the cell container 100 are not particularly limited and may be differentiated somatic cells or undifferentiated stem cells. The cells may be living cells or dead cells.

[0090] (well plate) Furthermore, the resin film described above can be attached to another member to form a part of the cell container.

[0091] 3 is a perspective view of a culture vessel 300, which is a cell vessel having a resin film. FIG. 4A is a cross-sectional view of a portion of the culture vessel 300.

[0092] The culture vessel 300 is used, for example, to culture cells derived from a human. Such a culture vessel 300 has a plurality of storage sections 310 (see FIG. 4A) for culturing cells. The culture vessel 300 is housed in a culture space of a culture device (for example, an incubator) with a drug (culture medium) and cells to be cultured (hereinafter referred to as "target cells") stored in the storage sections 310.

[0093] The culture vessel 300 can be suitably used for culturing spheroids (cell aggregates) of target cells. Note that the culture vessel 300 does not need to be used in a state housed in a culture device. The culture vessel 300 may be used in various situations depending on the target cells.

[0094] The culture vessel 300 has a base material 320 and a resin film 330 .

[0095] The substrate 320 has a frame 322 and a plurality of wells 324. The substrate 320 is made of, for example, a synthetic resin and is an integrally molded product produced by injection molding. Examples of synthetic resins that form the substrate 320 include polystyrene and polyolefin. Examples of the polyolefin include cyclic olefin (co)polymers, 4-methyl-1-pentene (co)polymers, polypropylene, and polyethylene. Of these, 4-methyl-1-pentene polymers are preferred from the viewpoint of increasing the gas permeability of the substrate 320. Alternatively, 4-methyl-1-pentene polymers, polystyrene, polypropylene, and cyclic olefin polymers are preferred from the viewpoint of increasing the heat resistance of the substrate 320. As the 4-methyl-1-pentene polymer, the 4-methyl-1-pentene polymer described as the material for the resin film can be used in the same way, but a polymer different from the material for the adhesive layer may also be used.

[0096] The frame 322 is made up of a rectangular frame-shaped member. The well 324 is provided in a space surrounded by the frame 322. The well 324 is provided integrally with the frame 322.

[0097] The wells 324 are cylindrical and open upward. In this embodiment, the shape of the opening of the wells 324 (in other words, the outer shape in a plan view) is circular. The wells 324 are arranged side by side in the left-right and front-rear directions. The number of wells 324 may be, for example, 6, 24, 96, or 384. Of course, the number of wells 324 may be other than 6, 24, 96, and 384.

[0098] Adjacent wells 324 are connected to each other by connecting portions 326. The left-end well 324, the right-end well 324, the front-end well 324, and the rear-end well 324 are connected to the inner circumferential surface of the frame portion 322 via connecting portions (not shown).

[0099] The base material 320 has a bottom plate portion 328 on its bottom surface. The bottom plate portion 328 is a plate-like member that forms the bottom surface of the wells 324 and connects the lower ends of the wells 324 together. The outer edge of the bottom plate portion 328 is connected to the inner circumferential surface of the frame portion 322 along its entire periphery.

[0100] Resin film 330 is a lid material for culture vessel 300. Resin film 330 seals the inside of well 324, which is a recess, and prevents liquid components such as culture fluid from volatilizing and liquid components from leaking from well 324 due to vibration of culture vessel 300, etc.

[0101] The resin film 330 may be a single-layer film or a multi-layer film.

[0102] FIG. 4B is a cross-sectional view of a portion of the culture vessel 300 shown in FIG. 3, showing another embodiment of the culture vessel.

[0103] 4B, the culture vessel 300 has a base material 320 having through-hole-shaped wells 324. A resin film 330a serving as a cover material is adhered to one opening (top surface) of the well 324, and a resin film 330b serving as a base material is adhered to the other opening (bottom surface).

[0104] In the configuration shown in FIG. 4B, the resin film may be adhered to only one of the openings of well 324, and another resin sheet may be adhered or bonded to the other opening.

[0105] (Culture vessel for nerve cells) Fig. 5A is a bottom perspective view showing another culture vessel 500 having a resin film. Fig. 5B is a top perspective view of the culture vessel 500. Fig. 6 is a cross-sectional view of a portion of the culture vessel 500.

[0106] Culture vessel 500 is used, for example, for culturing neurons. Such culture vessel 500 has chambers 524a and 524b in which cell bodies 540 of undeveloped neurons induced to differentiate from stem cells are placed, and a channel 524c that connects chambers 524a and 524b. Culture vessel 500 is placed in the culture space of a culture device (e.g., an incubator) with a drug (culture solution) contained in chambers 524a, 524b, and channel 524c, and with a mass of cell bodies 540 of neurons, which are target cells to be cultured, contained in one of chambers 524a and 524b.

[0107] Cell bodies 540 housed in the chamber grow axons 542 along flow channels 524c, respectively. At this time, axons 542 self-organize and bundle together to form nerve tissue.

[0108] The culture vessel 500 has a base material 520 and a resin film 530 .

[0109] Base material 520 has frame portion 522 and bottom plate portion 526. At positions of bottom plate portion 526 corresponding to chamber 524a, chamber 524b, and flow path 524c, through-holes are formed in the shapes of chamber 524a, chamber 524b, and flow path 524c. Base material 520 is an integrally molded product made by injection molding from the same synthetic resin as base material 320, for example.

[0110] 5A, resin film 530 is placed on the bottom surface of substrate 520 (the bottom surfaces of chambers 524a, 524b, and flow path 524c) and pressed to adhere to the bottom surface of substrate 520. The method of pressing is not particularly limited, and resin film 530 may be pressed against the bottom surface of substrate 520 with a finger, or resin film 530 may be pressed against the bottom surface of substrate 520 using a member such as a roll. The strength of the pressure may be about the same as described above.

[0111] This forms culture vessel 500 having resin film 530 as the base material of chamber 524a, chamber 524b, and channel 524c. At this time, resin film 530 slightly penetrates into the through-hole, and thickness T1 at the periphery of the through-hole is thinner than thickness T2 at the center of the through-hole.

[0112] The resin film 530 may be a single-layer film or a multi-layer film.

[0113] 3. Other embodiments It should be noted that each of the above-described embodiments represents an example of the present invention, and the present invention is not limited to the above-described embodiments. It goes without saying that various other embodiments are possible within the scope of the concept of the present invention.

[0114] For example, the cell container described above is a culture container for growing or propagating cells, but a container for storing or transporting cells or for observing cells may also be a cell container having a resin film. Furthermore, the cells targeted by the cell container are not particularly limited, and may be differentiated somatic cells or undifferentiated stem cells. The cells may be living cells or dead cells.

[0115] Furthermore, the use of the resin film is not limited to cell containers, and it may also be used to produce containers for other purposes. [Example]

[0116] The present invention will be described in detail based on examples, but the present invention is not limited to these examples.

[0117] 1. Prepare ingredients 1-1,4-methyl-1-pentene copolymer The following 4-methyl-1-pentene copolymer was prepared.

[0118] 1-1-1, 4-methyl-1-pentene copolymer 1 (4MP1-1) A copolymer consisting of 85 mol% of structural units derived from 4-methyl-1-pentene and 15 mol% of structural units derived from propylene. The melting point is 130°C.

[0119] 1-1-2,4-methyl-1-pentene copolymer 2 (4MP1-2) A copolymer containing 98.2 mol% of structural units derived from 4-methyl-1-pentene and 1.8 mol% of structural units derived from 1-hexadecene and 1-octadecene. The melting point is 228°C. There is one CFC elution peak.

[0120] 1-1-3, 4-methyl-1-pentene copolymer 3 (4MP1-3) A copolymer consisting of 97.60 mol% of structural units derived from 4-methyl-1-pentene, 1.44 mol% of structural units derived from 1-hexadecene, and 0.96 mol% of structural units derived from 1-octadecene. The melting point is 224°C. There is one CFC elution peak.

[0121] 1-1-4, 4-methyl-1-pentene copolymer 4 (4MP1-4) A copolymer containing 92.7 mol% of structural units derived from 4-methyl-1-pentene and 6.8 mol% of structural units derived from 1-decene, produced by the method described in Production Example 1. Melting points are 227°C and 160°C. There are three CFC elution peaks.

[0122] (Production Example 1) In accordance with the polymerization method described in Example 1 (paragraph

[0153] ) of WO 2017 / 150265, copolymers (A-1), (B-1) and (C-1) of 4-methyl-1-pentene and 1-decene were obtained by changing the proportions of 4-methyl-1-pentene, 1-decene and hydrogen used so that the physical properties of the resulting 4-methyl-1-pentene copolymers would be the values ​​listed in Table 1.

[0123] [Table 1]

[0124] 35 parts by mass of the copolymer (A-1), 45 parts by mass of copolymer (B-1), and 20 parts by mass of copolymer (C-1) were added to a twin-screw extruder (manufactured by Ikegai Corporation, PCM43, screw diameter: 43 mm), and then melt-kneaded at 280°C and a rotation speed of 200 rpm to obtain 4-methyl-1-pentene polymer 4 (4MP1-4).

[0125] The composition ratio and melting point of the 4-methyl-1-pentene copolymer were measured by the following methods.

[0126] (composition ratio) A nuclear magnetic resonance spectrometer (ECP500 manufactured by JEOL Ltd.) was used. The solvent was a mixed solvent of o-dichlorobenzene / heavy benzene (80 / 20% by volume), the sample concentration was 55 mg / 0.6 mL, the measurement temperature was 120 °C, the observation nucleus was 13 C (125 MHz), the sequence was single pulse proton decoupling, the pulse width was 4.7 μs (45° pulse), the repetition time was 5.5 s, the number of accumulations was 10,000 or more, and 27.50 ppm was used as the reference value for the chemical shift. 13 A C-NMR spectrum was obtained, and the amounts of structural units derived from 4-methyl-1-pentene and structural units derived from other α-olefins were determined from the spectrum.

[0127] (Melting Point) The exothermic and endothermic curves were measured using a differential scanning calorimeter (DSC measuring device) (Seiko Instruments Inc., DSC220C), and the temperature at the maximum melting peak during heating was taken as the melting point (Tm). Approximately 5 mg of sample was placed in a measurement aluminum pan and heated from 20°C to 280°C at a heating rate of 10°C / min. After holding at 280°C for 5 minutes, the sample was cooled to 20°C at a cooling rate of 10°C / min and held at 20°C for 5 minutes. The sample was then heated again from 20°C to 280°C at a heating rate of 10°C / min and cooled again to 50°C at a cooling rate of 50°C / min. The melting peak that appeared during the second heating was taken as the melting point (Tm). When multiple melting peaks were present, each peak temperature was taken as the melting point (Tm).

[0128] (Cross fractionation chromatography (CFC) measurement) Using orthodichlorobenzene as a solvent, the elution start temperature (temperature at which the cumulative elution weight % becomes 0.5% by weight) measured by cross fractionation chromatography (CFC) under the following conditions is defined as [TS], and the elution end temperature (temperature at which the cumulative elution weight % becomes 99% by weight) is defined as [TE]. The presence of two or more elution peaks CFC, which are peaks that appear on the differential elution curve obtained by differentiating the cumulative elution curve obtained in the elution temperature range from TE to TS, was confirmed. <Condition> Equipment: Cross-fractionation chromatograph CFC2 (Polymer ChAR) Detector (built-in): Infrared spectrophotometer IR4 (Polymer ChAR) Detection wavelength: 3.42 μm (2,920 cm-1); fixed Sample concentration: 30 mg / 30 mL, injection volume: 0.5 mL Cooling time: 1.0℃ / min Elution category: 4.0℃ interval (-20℃~140℃) GPC column: Shodex HT-806M x 3 (product name, manufactured by Showa Denko) GPC column temperature: 145℃ GPC column calibration: Monodisperse polystyrene (Tosoh Corporation) Molecular weight calibration method: General calibration method (polystyrene equivalent) Mobile phase: o-dichlorobenzene (with BHT) Flow rate: 1.0mL / min.

[0129] 1-2.Butene copolymer The following butene copolymers were prepared.

[0130] 1-2-1. Butene-based copolymer 1 (PB1) Mitsui Chemicals, Inc., Toughmer BL2491M (butene-propylene copolymer, density 0.90 kg / cm 3 The melt flow rate (MFR) measured at 230°C under a load of 2.16 kgf was 9 g / 10 min.

[0131] 1-2-2. Butene-based copolymer 2 (PB2) Mitsui Chemicals, Inc., Toughmer BL3450M (butene-ethylene copolymer, density 0.90 kg / cm 3 The melt flow rate (MFR) measured at 230°C under a load of 2.16 kgf was 9 g / 10 min.

[0132] 2. Resin film manufacturing The materials were fed into the hopper of a multilayer film molding machine equipped with a multilayer T-die (lip width 350 mm), two single-screw extruders with a cylinder inner diameter of 20 mm (for outer layer 1, a non-heat-sealable layer, and outer layer 2, a heat-sealable layer), and one single-screw extruder with a cylinder inner diameter of 25 mm (for the core layer). The cylinder temperatures (feed section / compression section to metering section) of each extruder were 270°C / 295°C for the extruder for outer layer 1, 140°C / 250°C for the extruder for outer layer 2, and 240°C / 285°C for the extruder for the core layer. The die temperature was 290°C. Melt-kneaded mixtures of the resin materials for outer layer 1, core layer, and outer layer 2 were coextruded through the T-die to achieve the composition and film thickness of each layer listed in Table 1. The mixtures were cast using a mirror-finished chill roll set at 20°C and then withdrawn to obtain a resin film (total thickness: 100 μm).

[0133] 3. Measurement For each film, oxygen permeability and oxygen permeability coefficient, carbon dioxide permeability and carbon dioxide permeability coefficient, moisture permeability, total light transmittance, haze, and heat sealability were measured by the following methods.

[0134] 3-1. Oxygen permeability, oxygen permeability coefficient, carbon dioxide permeability and carbon dioxide permeability coefficient In accordance with JIS K 7126-1:2006, a differential pressure gas permeability measuring device (manufactured by Toyo Seiki Seisakusho) was used to measure the gas permeability of a multilayer film over a 5cm2 area at a test temperature of 23°C and a test humidity of 0%RH. 2 The oxygen permeability and carbon dioxide permeability were measured as follows. The measurement area was adjusted by preparing two Modern Control Co., Ltd. adhesive aluminum masks with a 25 mm diameter hole in the center, and stacking the resin film to be measured so that it was sandwiched between the center holes of the two masks. The oxygen permeability and carbon dioxide permeability coefficients were calculated by multiplying the measured oxygen permeability and carbon dioxide permeability by the total thickness of the resin film, respectively.

[0135] 3-2. Moisture permeability Measurement was performed under condition B (test temperature 40°C, test humidity 90% RH) in accordance with the isobaric method (cup type gravimetric method) described in JIS Z 0208:2021.

[0136] 3-3. Total light transmittance and haze Using a fully automatic haze meter (TC-HIII DPK, manufactured by Tokyo Denshoku Co., Ltd.), the total light transmittance and haze were measured in accordance with JIS K 7361-1:1997 and JIS K 7136:2000, respectively.

[0137] 3-4. Heat sealing properties (part 1) The resin film was cut with its MD direction as the machine direction, to prepare two rectangular heat-sealable test pieces measuring 200 mm wide x 60 mm long. The two heat-sealable test pieces were stacked with their outer layers 2 facing each other, and then heat-sealed using a heat-sealing tester (Tester Sangyo Co., Ltd., Thermal Gradient Heat-Sealing Tester TP-701-G) under the following conditions: upper and lower temperatures (heat-sealing temperature) of 160°C, seal width of 10 mm, seal pressure of 0.1 MPa, and seal time of 2 seconds. The heat-sealing was performed so that the weld line was perpendicular to the MD direction of the multilayer film. The two welded multilayer films were then removed from the tester, and a 15 mm wide strip of test piece was cut out perpendicular to the weld line to prepare the heat-sealable test piece. Using a tensile testing machine (Orientec Co., Ltd., Tensilon universal material testing machine, model RTG-1250), the heat-sealed test specimen was pulled in a direction parallel to the longitudinal direction of the test specimen and peeled at a test temperature of 23°C, a chuck distance of 50 mm, and a pulling speed of 300 mm / min. The maximum peel strength and the elongation at peeling relative to the chuck distance were measured and recorded as the heat-seal strength (unit: N / 15 mm) and the elongation at peeling (unit: %), respectively. The heat-seal strength and the elongation at peeling were measured for 35 heat-sealed test specimens, and the average values ​​were calculated.

[0138] 3-5. Heat sealing properties (part 2) A compression molding machine (Shinto Metal Industries, ASF-10, maximum clamping force 10 ton) and a cooling compression molding machine (Shinto Metal Industries, NSF-37, maximum clamping force 37 ton) were used to produce a press sheet measuring 65 mm wide x 65 mm long x 1 mm thick from 4MP1-2. The heating temperature was 270°C, the preheating time was 10 minutes, the pressurization time was 3 minutes, and the cooling time was 5 minutes.

[0139] The outer layer 2 (sealant layer) of the resin film was placed on the surface of the press sheet, and the resulting structure was heat-sealed using a heat seal tester (Tester Sangyo Co., Ltd., thermal gradient heat seal tester, model TP-701-G) under the following conditions: upper heat seal bar temperature (heat seal temperature) 240°C, lower heat seal bar temperature 23°C, seal width 10 mm, seal pressure 0.1 MPa, and seal time 2 seconds. The heat seal was performed so that the weld line was perpendicular to the MD direction of the multilayer film. The welded multilayer film and press sheet were then removed from the tester, and 15 mm wide strips were cut out in the direction perpendicular to the weld line to serve as heat-sealed test pieces. Using a tensile testing machine (Orientec Co., Ltd., Tenshin Universal Testing Machine, Model RTG-1250), the welded strip-shaped test pieces were pulled in a direction parallel to the longitudinal direction of the test piece and peeled under conditions of a test temperature of 23°C, a chuck distance of 50 mm, and a pulling speed of 300 mm / min. The peel strength and the maximum elongation at peeling relative to the chuck distance were measured and recorded as the peel strength (unit: N / 15 mm) and the elongation at peeling (unit: %), respectively. Peel strength and elongation at peeling were measured for three test pieces and average values ​​were calculated.

[0140] Table 2 shows the structures and measurement results of Films 1 to 4.

[0141] [Table 2]

[0142] The results shown in Table 2 show that a resin film having a layer containing a 4-methyl-1-pentene copolymer in which the content of structural units derived from 4-methyl-1-pentene is 90 mol% or more and 96 mol% or less and the content of structural units derived from α-olefins (excluding 4-methyl-1-pentene) having 6 to 20 carbon atoms is 4 mol% or more and 10 mol% or less has a large breaking elongation in the welded portion. [Industrial Applicability]

[0143] The resin film according to the present invention can be applied to various types of cell containers and other uses. [Explanation of symbols]

[0144] 100 cell containers 110 Bag section 112, 114 Resin film 116 Adhesion part 200 Port parts 300 Culture vessels 310 Storage unit 320 Base material 322 Frame 324 wells 326 Connection 328 Bottom plate part 330, 330a, 330b resin film 500 culture vessels 520 Base material 522 Frame 526 Bottom plate part 524a, 524b chambers 524c Channel 530 Resin film 540 cell body 542 axons 544 Nervous Tissue

Claims

1. a layer containing a 4-methyl-1-pentene copolymer in which the content of structural units derived from 4-methyl-1-pentene is 90 mol % or more and 96 mol % or less and the content of structural units derived from an α-olefin (excluding 4-methyl-1-pentene) having 6 to 20 carbon atoms is 4 mol % or more and 10 mol % or less; Oxygen permeability at 23°C is 3.0 L / (m ２ ・day・am) or more 100.0L / (m ２ ・day・am) or less, Resin film.

2. The layer comprises a mixture of two or more 4-methyl-1-pentene copolymers having different melting points as measured by differential scanning calorimetry (DCS); The resin film according to claim 1.

3. The layer contains a mixture of a 4-methyl-1-pentene copolymer having no melting point observed by differential scanning calorimetry (DCS) and a 4-methyl-1-pentene copolymer having a melting point observed by differential scanning calorimetry (DCS). The resin film according to claim 1.

4. The layer was analyzed by cross fractionation chromatography (CFC) using o-dichlorobenzene as an eluent, and the elution start temperature (the temperature at which the cumulative elution weight percentage becomes 0.5 weight percent) was set to [T Ｓ ], and the elution end temperature (the temperature at which the cumulative eluted weight percentage reaches 99% by weight) is [T Ｅ ], the elution temperature T Ｅ ~T Ｓ The 4-methyl-1-pentene copolymer mixture has two or more elution peaks, which are peaks that appear on a differential elution curve obtained by differentiating a cumulative elution curve obtained in the range of The resin film according to claim 1.

5. The total light transmittance is 70% or more and 100% or less. The resin film according to claim 1.

6. The haze is 0.1% or more and 10% or less. The resin film according to claim 1.

7. The thickness is 50 μm or more and 1 mm or less. The resin film according to claim 1.

8. A multilayer film, the ratio of the thickness of the layer containing the 4-methyl-1-pentene copolymer to the total thickness of the resin film is 50% or more and 90% or less; The resin film according to claim 1.

9. The ratio of the oxygen permeability coefficient to the carbon dioxide permeability coefficient (O ２ / CO ２ ) is 1 / 4 or more and 1 / 2.5 or less, The resin film according to claim 1.

10. A film having a layer containing the 4-methyl-1-pentene copolymer and a sealant layer. The resin film according to claim 1.

11. The heat seal strength after the sealant layers are heat-sealed at 160°C is 10 N / 15 mm or more and 100 N / 15 mm or less. The resin film according to claim 10.

12. The elongation at peeling after heat sealing the sealant layers together at 160°C is 100% or more and 500% or less. The resin film according to claim 10.

13. A container formed into a bag shape by heat-sealing one or more sheets of the resin film according to any one of claims 1 to 12.

14. a port member that communicates the inside and the outside of the container; The port member is bonded to the resin film by heat sealing.

14. The container of claim 13.

15. The cell container, 14. The container of claim 13.

16. Culturing cells inside the container of claim 15. Cell culture methods.

17. The resin film according to any one of claims 1 to 12, a substrate having a through-hole or a recess, the resin film is in close contact with the top surface or bottom surface of the through hole or the top surface of the recess; cell container.

18. Cultivating cells inside the cell container of claim 17. Cell culture methods.

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