Topical agent for skin

Abstract

To provide a topical agent for skin that is derived from a natural product, exhibits excellent biosafety, and exerts functions of promoting proliferation and activation of epidermal cells.SOLUTION: At least one selected from a fermented product of Coix lacryma-jobi seed, an extract of Salicornia europaea, or an extract of Aspalathus linearis is used as an active ingredient of an epidermal cell activator.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present invention relates to an external skin preparation that exhibits the function of proliferation and activation of epidermal cells. [Background technology]

[0002] It is known that factors such as ultraviolet rays, air pollutants, and dryness can damage the epidermis, reducing the activity of epidermal cells and disrupting cell turnover. Traditionally, various moisturizers (amino acids, glycerin, polyhydric alcohols, sodium lactate, etc.) have been proposed as ingredients to protect the epidermis, but they have had issues with stability and effectiveness. DISCLOSURE OF THE INVENTION [Problem to be solved by the invention]

[0003] In view of the problems of the conventional techniques, the present inventors have conducted extensive research to find new active ingredients derived from natural products that are safe for the skin. As a result, they have found that one or more of a fermented product of Job's tears seeds, a Salicornia herb extract, and an Aspalathus linearis extract have the effect of promoting the activation of epidermal cells.

[0004] It has been disclosed in Patent Documents 1 to 3, for example, that fermented products of Job's tears seeds, Salicornia herbacea extracts, and Aspalathus linearis extracts each have physiological skin activity, but it was not known that these fermented products or extracts have the effect of promoting the activation of epidermal cells. [Patent Document 1] Japanese Patent Application Laid-Open No. 2010-138139 [Patent Document 2] Japanese Patent Application Laid-Open No. 2005-145878 [Patent Document 3] Japanese Patent Application Laid-Open No. 2009-249371 [Means for solving the problem]

[0005] The present invention relates to an epidermal cell activator comprising any one or more of a fermented product of Job's tears seeds, a Salicornia herb extract, and an Aspalathus linearis extract. [Effects of the Invention]

[0006] The present invention provides an external skin preparation that promotes the activation of epidermal cells and normalizes the skin's barrier function and turnover by using as an active ingredient one or more of an extract of fermented Job's tears seeds, a Salicornia herb extract, and an Aspalathus linearis extract. [Brief explanation of the drawings]

[0007] [Figure 1] FIG. 1 shows the effect of the extract according to the present invention in promoting flattening of epidermal cells. DETAILED DESCRIPTION OF THE INVENTION

[0008] In the present invention, "jobi's tears" refers to a plant of the genus Job's tears in the family Poaceae, and may be any species. The part used for fermentation is preferably the seeds, and either hulled or unhulled seeds may be used. Furthermore, the seeds may be whole grains, powder obtained by crushing or crushing, or grains or powder of Job's tears seeds that have been treated at high temperature and under high pressure.

[0009] Yeast is preferably used as the fungus used for fermenting Job's tears, and preferred yeasts are those of the genus Saccharomyces, such as Saccharomyces cerevisiae, Saccharomyces awamori, Saccharomyces chevalieri, Saccharomyces carlsbergensis, and Saccharomyces bayonus.

[0010] The Salicornia used in the present invention is a plant of the genus Salicornia in the family Chenopodiaceae, and is sometimes called coral grass. Examples include Salicornia europaea, Salicornia bigelowii, and Salicornia dolichostachia ssp. strictissima. The whole plant is preferably used for extraction.

[0011] The Aspalathus linearis used as the raw material for the Aspalathus linearis extract of the present invention is a plant belonging to the genus Aspalathus in the family Fabaceae, and is preferably non-fermented and green (so-called green rooibos).The part used for extraction is preferably the whole plant or leaves.

[0012] The extract is prepared by first washing the part of each plant to remove foreign matter if necessary, leaving it as is or dried, and then shredding or crushing it as necessary, and contacting it with an extracting solvent to carry out extraction. Extraction can be carried out by contacting it with an extracting solvent according to a conventional method such as the immersion method.

[0013] Examples of extraction solvents include water; lower alcohols such as methanol, ethanol, and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol, and glycerin; esters such as ethyl acetate, butyl acetate, and methyl propionate; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; and hydrocarbon solvents such as n-hexane, toluene, and chloroform, and these may be used alone or in combination.

[0014] Among the above-mentioned extraction solvents, hydrophilic solvents such as water, lower alcohols, or polyhydric alcohols are preferred in the present invention from the viewpoints of the efficacy of the resulting extract, skin irritation, and the wide applicability of these solvents to topical skin preparations (cosmetics, quasi-drugs, etc.). Preferred examples of hydrophilic solvents include the use of water, lower alcohols (particularly ethanol), or polyhydric alcohols (particularly 1,3-butylene glycol) alone, or a mixed solvent of water and a lower alcohol (particularly ethanol), or a mixed solvent of water and a polyhydric alcohol (particularly 1,3-butylene glycol, glycerin). Among these, water alone or a mixed solvent of water and 1,3-butylene glycol is particularly preferred.

[0015] When a mixed solvent is used, the mixing ratio is preferably in the range of, for example, a volume ratio (same hereinafter) of 1:99 to 99:1 for a mixed solvent of water and 1,3-butylene glycol, 1:25 to 25:1 for a mixed solvent of water and ethanol, or 1:20 to 20:1 for a mixed solvent of water and glycerin.

[0016] The weight ratio of the part of each plant to be used to the extraction solvent is generally 1:1 to 1:50.

[0017] When preparing the extract solution, the pH is not particularly limited, but is generally preferably in the range of 3 to 9. In this sense, if necessary, the extract solvent may be blended with an alkalinity adjuster such as sodium hydroxide, sodium carbonate, or potassium hydroxide, or an acidity adjuster such as citric acid, hydrochloric acid, phosphoric acid, or sulfuric acid to adjust the pH to the desired level.

[0018] The extraction conditions, such as extraction temperature and extraction time, vary depending on the type and pH of the solvent used. For example, when water, 1,3-butylene glycol, or a mixture of water and 1,3-butylene glycol is used as the solvent, the extraction temperature is preferably in the range of 0°C to 80°C, more preferably in the range of 0°C to 20°C, and the extraction time is preferably in the range of 1 to 168 hours (1 hour to 1 week), more preferably in the range of 1 to 120 hours (1 hour to 5 days).

[0019] The extract solutions prepared as described above are mixed and generally adjusted to a pH of 3 to 8. The mixture may be used as an ingredient in a topical skin preparation as is, or may be used at a desired concentration by vacuum concentration, etc. The extract may also be dried by a conventional method such as spray drying.

[0020] The solid content concentration of the Job's tears fermented solution according to the present invention is preferably 0.1 to 5.0% by weight in the fermented solution. The solid content concentration of the Salicornia extract solution according to the present invention is preferably 0.1 to 5.0% by weight in the extract, and the solid content concentration of the Aspalathus linearis extract solution according to the present invention is preferably 0.1 to 5.0% by weight in the extract.

[0021] When the fermented product and extract according to the present invention are incorporated into an external skin preparation, the solid content concentration is preferably 0.001 to 10% by weight or more.

[0022] Examples of topical skin preparations (cosmetics, quasi-drugs, etc.) containing the composition of the present invention include cleansing cosmetics such as emulsions, creams, lotions, essences, transparent gels, packs, lipsticks, foundations, sheet masks, liquid foundations, makeup press powders, blushers, face powders, facial cleansers, body shampoos, hair shampoos, and soaps.

[0023] When the fermented product and extract according to the present invention are incorporated into topical skin preparations (cosmetics, quasi-drugs, etc.), ingredients used in topical skin preparations, such as oily ingredients, surfactants (synthetic or natural), moisturizers, thickeners, emulsifiers or emulsifier aids, preservatives / bactericides, powder ingredients, UV absorbers, antioxidants, pigments, fragrances, anti-wrinkle agents, and other physiologically active ingredients, can be appropriately incorporated as needed. Furthermore, as long as the efficacy and features of the composition according to the present invention are not impaired, there is no problem in incorporating the fermented product and extract into topical skin preparations in combination with other physiologically active ingredients.

[0024] Examples of oily components include olive oil, jojoba oil, castor oil, soybean oil, rice oil, rice germ oil, coconut oil, palm oil, cocoa oil, meadowfoam oil, shea butter, tea tree oil, avocado oil, macadamia nut oil, bergamot oil, lavender oil, rose oil, bergamot oil, chamomile oil, and other plant-derived oils and fats such as squalane; vitamin A oil; animal-derived oils and fats such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, petrolatum, paraffin wax, Examples include hydrocarbons such as squalane; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, and cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, pantothenyl alcohol, and stearyl alcohol; and synthetic esters and synthetic triglycerides such as isopropyl myristate, isopropyl palmitate, butyl oleate, 2-ethylhexyl glyceride, and higher fatty acid octyldodecyl (e.g., octyldodecyl stearate).

[0025] Examples of surfactants include nonionic surfactants such as polyoxyethylene alkyl ethers, polyoxyethylene fatty acid esters, polyoxyethylene sorbitan fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, polyoxyethylene glycerin fatty acid esters, polyoxyethylene hydrogenated castor oil, and polyoxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene Examples of surfactants that can be used include anionic surfactants such as ethylene alkyl phenyl ether phosphates; cationic surfactants such as quaternary ammonium salts, primary to tertiary fatty amine salts, trialkylbenzylammonium salts, alkylpyridinium salts, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salts, N,N-dialkylmorpholinium salts, and polyethylene polyamine fatty acid amide salts; and amphoteric surfactants such as N,N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N,N,N-trialkyl-N-alkyleneammoniocarboxybetaine, N-acylamidopropyl-N', N'-dimethyl-N'-β-hydroxypropylammoniosulfobetaine.

[0026] Examples of emulsifiers and / or emulsifying aids that can be blended include stevia derivatives such as enzyme-treated stevia, saponin or derivatives thereof, casein or its salts (sodium, etc.), sugar and protein complexes, sucrose or esters thereof, lactose, soybean-derived water-soluble polysaccharides, soybean-derived protein and polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (enzyme-treated stevia, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.), saponin and derivatives thereof, lecithin and derivatives thereof (hydrogenated lecithin, etc.), lactic acid bacteria-fermented rice, lactic acid bacteria-fermented germinated rice, lactic acid bacteria-fermented grains (wheat, beans, millet, etc.), etc.

[0027] Examples of moisturizing agents include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate, and the like, as well as sugars such as trehalose and raffinose, mucopolysaccharides (e.g., hyaluronic acid or its salts or derivatives, hyaluronic acid hydrolyzate, chondroitin or its derivatives, heparin or its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, lactic acid, urea, and higher fatty acid octyldodecyl.

[0028] Examples of thickeners include components derived from brown algae, green algae, or red algae, such as alginic acid, agar, carrageenan, and fucoidan; polysaccharides such as pectin and aloe polysaccharide; gums such as tragacanth gum, locust bean gum, xanthan gum, and guar gum; cellulose derivatives such as carboxymethyl cellulose, hydroxyethyl cellulose, and hydroxypropyl cellulose; synthetic polymers such as carboxyvinyl polymers, alkyl-modified carboxyvinyl polymers, polyvinyl alcohol, polyvinylpyrrolidone, and acrylic acid-methacrylic acid copolymers; hyaluronic acid and its derivatives; polyglutamic acid and its derivatives, and polyacrylic acid.

[0029] Anti-inflammatory agents include allantoin, dipotassium glycyrrhizinate, monoammonium glycyrrhizinate, β-glycyrrhetinic acid, stearyl glycyrrhetinate, ε-aminocaproic acid, d-camphor, dl-camphor, zinc oxide, panthenol, pyridoxine hydrochloride, and riboflavin or a derivative thereof.

[0030] Examples of antiseptics and disinfectants include urea; benzoic acid or its salts, parahydroxybenzoic acid esters such as methyl parahydroxybenzoate, ethyl parahydroxybenzoate, propyl parahydroxybenzoate, and butyl parahydroxybenzoate; phenoxyethanol, dichlorophene, hexachlorophene, chlorhexidine hydrochloride, benzalkonium chloride, salicylic acid, sodium salicylate, zinc pyrithione, benzalkonium chloride, ethanol, undecylenic acid, phenols, alkylisoquinolinium bromide, resorcinol, jamal (imidazoline urea), isopropylmethylphenol, triclosan, trichlorocarbanide, trichlorohydroxydiphenol ether, hinokitiol, 1,2-pentanediol, propanediol, hexanediol, concentrated benzalkonium chloride solution 50, and essential oils such as peppermint oil and eucalyptus oil.

[0031] Examples of cell activators include pantothenyl alcohol, menthol, dl-menthol, and γ-oryzanol.

[0032] Anti-acne agents include sulfur, salicylic acid or its salts, photosensitizer No. 201, pyridoxine dicaprylate, and the like.

[0033] Examples of powder components include sericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, cellulose-based powder, powder of grains (rice, wheat, corn, millet, etc.), powder of beans (soybean, adzuki bean, etc.), etc.

[0034] Examples of ultraviolet absorbers include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl cinnamate, oxybenzone, 2,4-dihydroxybenzophenone, 2-hydroxy-4-methoxybenzophenone-5-sulfonate, 4-tert-butyl-4-methoxybenzoylmethane, 2-(2-hydroxy-5-methylphenyl)benzotriazole, urocanic acid, ethyl urocanate, and aloe extract.

[0035] Examples of antioxidants include butylhydroxyanisole, butylhydroxytoluene, propyl gallate, carotenoids such as astaxanthin, vitamin E and its derivatives (e.g., tocopherol acetate, tocopherol nicotinate), vitamin A and its derivatives (retinol palmitate, etc.), and the like.

[0036] Further, examples of whitening agents include one or more selected from ellagic acid and its derivatives, resorcinol derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acids, nicotinic acid derivatives, and AMP (adenosine monophosphate, adenosine monophosphate).

[0037] Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol; examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid, and 2-hydroxy-5-propionyloxybenzoic acid; and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid. Examples include one or more selected from kojic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4-methoxysalicylic acid potassium salt, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, alpha-hydroxy acid, AMP (adenosine monophosphate, adenosine monophosphate), t-cycloamino acid derivatives, mulberry bark extract, chamomile extract, hydrolyzed rice bran extract, saxifrage extract, and white mustard extract or hydrolyzates thereof.

[0038] Examples of the kojic acid derivatives include kojic acid esters such as kojic acid monobutyrate, kojic acid monocaprate, kojic acid monopalmitate, and kojic acid dibutyrate, kojic acid ethers, and kojic acid sugar derivatives such as kojic acid glucoside. Examples of the ascorbic acid derivatives include ascorbic acid ester salts such as sodium L-ascorbic acid 2-phosphate, magnesium L-ascorbic acid 2-phosphate, sodium L-ascorbic acid 2-sulfate, and magnesium L-ascorbic acid 2-sulfate; Ascorbic acid sugar derivatives such as ascorbic acid 2-glucoside, L-ascorbic acid 5-glucoside, ascorbyl tocopheryl maleate, ascorbyl tocopheryl phosphate K, myristyl 3-glyceryl ascorbate, caprylyl 2-glyceryl ascorbate, etc.; 6-acylated products of these ascorbic acid sugar derivatives (the acyl group is hexanoyl, octanoyl, decanoyl, etc.); L-ascorbic acid tetraisopalmitate, L-ascorbic acid tetralaurate, etc.; L-ascorbic acid tetrafatty acid esters such as 3-isopalmitate, ... -O-ethyl ascorbic acid, L-ascorbic acid-2-phosphate-6-O-palmitate sodium, glyceryl ascorbic acid or its acylated derivatives, ascorbic acid glycerin derivatives such as bisglyceryl ascorbic acid, L-ascorbic acid aminopropyl phosphate, hyaluronic acid derivatives of L-ascorbic acid, 3-OD lactose-L-ascorbic acid, isostearyl ascorbyl phosphate, etc. Hydroquinone derivatives include arbutin (hydroquinone-β-D-glucopyranoside), α-arbutin (hydroquinone-α-D-glucopyranoside), Examples of tranexamic acid derivatives include tranexamic acid esters (e.g., tranexamic acid lauryl ester, tranexamic acid hexadecyl ester, tranexamic acid cetyl ester or a salt thereof), and tranexamic acid amides (e.g., tranexamic acid methylamide). Examples of resorcinol derivatives include 4-n-butylresorcinol and 4-isoamylresorcinol. Examples of 2,5-dihydroxybenzoic acid derivatives include 2,5-diacetoxybenzoic acid, 2-acetoxy-5-hydroxybenzoic acid,Examples of nicotinic acid derivatives include nicotinamide (niacinamide) and benzyl nicotinate, and examples of α-hydroxy acids include lactic acid, malic acid, succinic acid, citric acid, and α-hydroxyoctanoic acid.

[0039] Anti-wrinkle agents include vitamin A or its derivatives, niacinamide, vitamin E or its derivatives (tocopherol acetate, etc.), vitamin C or its derivatives (ascorbic acid glucoside, 3-O-ethyl ascorbic acid, ascorbic acid phosphate magnesium salt, etc.), pantothenyl alcohol, tranexamic acid, etc.

[0040] Furthermore, it is also possible to use in combination components derived from natural products such as the following plants or microorganisms: collagen or hydrolysates thereof, yeast extracts or hydrolysates, lactic acid bacteria cultures, grasses, cruciferous plants, Theaceae plants, Rosaceae plants, Paeoniaceae plants, Rutaceae plants, Amaranthaceae plants, Zosteraea plants, Leguminosae plants, Asteraceae plants, Fabaceae plants, Malvaceae plants, Gentianaceae plants, Lamiaceae plants, Nelumbosacaceae plants, Cucurbitaceae plants, Araliaceae plants, Solanaceae plants, Bignoniaceae plants, Actinidiaceae plants, Mulberry plants, Iridaceae plants, Campanulaceae plants, Oleaceae plants, Actinidiaceae plants, Mulberry plants, Rhamnaceae plants, Orchidaceae plants, and Anacardiaceae plants. Examples of suitable extracts include extracts of one or more plants selected from the family Garcinia, Valenciaceae, Rutaceae, Myrtaceae, Liliaceae, Crassulaceae, Cupressaceae, Convolvulaceae, and Asparagaceae, or hydrolysates or fermented products thereof; extracts of one or more seaweeds selected from the family Laminaria, Mirrataceae, and Ulvulaceae, or hydrolysates or fermented products thereof; jellyfish (autolyzed products of moon jellyfish, Nomura's jellyfish, etc.); hydrolysates or fermented products of hyaluronic acid; and extracts of royal jelly, or hydrolysates or fermented products thereof.

[0041] The present invention will now be described in more detail with reference to Production Examples, Test Examples, and Formulation Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and all % mean % by weight.

[0042] Production Example 1. Preparation of fermented Job's tears solution 50 g of husk-removed Job's tears seeds were crushed and 950 g of purified water was added to prepare a suspension, which was then heat-sterilized. 7 The cells / mL were inoculated and statically cultured for 3 days at 37° C. After the culture was completed, the mixture was sterilized by heating, cooled to room temperature, and filtered to obtain 500 g of a fermented Job's tears seed solution (solid concentration 1.01%).

[0043] Preparation Example 2: Preparation of Salicornia herb extract solution 200 g of purified water was added to 20 g of dried, shredded Salicornia herbacea whole plant, and the mixture was extracted for 1 hour at 40° C. The resulting extract was filtered to obtain 155 g of a brown, transparent extract solution (solid content: 1.98%).

[0044] Preparation Example 3. Preparation of Aspalathus linearis extract solution 100g of dried whole non-fermented rooibos from Aspalathus linearis, a plant of the Aspalathus genus of the Leguminosae family, was added to 1000g of a mixed solvent of purified water and 1,3-butylene glycol, and extracted at 80°C for 2 hours. After removing insoluble matter by filtration, 1028g of dark brown green rooibos extract (solid concentration 1.52%) was obtained.

[0045] Test Example 1. Effect of promoting epidermal cell proliferation NHEK epidermal cells were seeded onto a 96-well plate and cultured in a culture medium for one day under standard epidermal cell culture conditions. The fermented product of Production Example 1 was then added as a sample solution, and the culture was continued for an additional two days. The concentration of the sample solution was adjusted so that the final concentration in the culture medium was 0.7%. After the culture was completed, each plate was washed once with phosphate-buffered saline, and DNA was fluorescently stained with Hoechst 33342. The fluorescence intensity (Ex = 355 nm, Em = 460 nm) was measured to determine the DNA content. In parallel, a control solution containing 1,3-butylene glycol (30BG) at the same concentration as the sample solution was added instead of the sample solution, and the fluorescence intensity was measured in the same manner. The relative fluorescence intensity of the sample solution-added group relative to the control group was calculated and used as the cell proliferation rate (%).

[0046] The results of Test Example 1 are shown in Table 1. [Table 1] JPEG2025180829000002.jpg30109

[0047] As shown in Table 1, the fermented product of the present invention (the fermented product of Production Example 1) is suggested to significantly promote epidermal cell proliferation and activate epidermal cells, thereby activating epidermal turnover and leading to healthy skin conditions.

[0048] Test Example 2: Evaluation test of intracellular acidification in epidermal cells NHEK epidermal cells were seeded onto a 96-well plate and cultured in a culture medium under standard epidermal cell culture conditions. After one day of culture, the cells were allowed to incorporate BCECF-AM (3'-O-Acetyl-2',7'-bis(carboxyethyl)-4 or 5-carboxyfluorescein, diacetoxymethyl ester) reagent, and the extract from Preparation Example 2 was then added as a sample solution. BCECF is a reagent whose fluorescence intensity changes in correlation with the intracellular pH; the more acidic the pH, the lower the fluorescence intensity. The concentration of the sample solution in the above procedure was adjusted so that the final concentration of the solution in the culture medium was 0.2%. Fluorescence intensity (Ex = 355 nm, Em = 460 nm) was measured 3 hours after the addition of the sample solution. In parallel, the fluorescence intensity was measured in the same manner for the culture medium to which a 1,3-butylene glycol solution (30BG) of the same concentration as the sample solution was added as a control solution instead of the sample solution, and the relative value (%) of the fluorescence intensity in the sample solution-added area to the fluorescence intensity in the control area was calculated.

[0049] The results of Test Example 2 are shown in Table 2. [Table 2] JPEG2025180829000003.jpg30108

[0050] As shown in Table 2, it was confirmed that intracellular acidification was promoted in epidermal cells cultured in the presence of the extract according to the present invention (extract of Production Example 2). Intracellular acidification is a very important step in the differentiation of the granular layer into the stratum corneum, and there is a series of events in which intracellular acidification leads to the degradation of nuclear DNA and the formation of a normal stratum corneum. This suggests that the extract according to the present invention (extract of Production Example 2) promotes intracellular acidification, thereby activating epidermal cells and contributing to normal keratinization.

[0051] Test Example 3: Evaluation test of the effect of promoting flattening of epidermal cells NHEK epidermal cells were seeded onto a 96-well plate and cultured in culture medium under standard conditions for one day. The extract from Preparation Example 3 was then added as a sample solution, and the cells were cultured for an additional two days. The concentration of the sample solution was adjusted so that the final concentration of the solution in the culture medium was 0.1%. After the culture was completed, the morphology of the epidermal cells was observed. In parallel, the morphology of epidermal cells cultured in a culture medium to which a 1,3-butylene glycol solution (30BG) at the same concentration as the sample solution had been added as a control solution was also observed.

[0052] The results of Test Example 3 are shown in Figure 1. Figure 1(a) shows the morphology of epidermal cells when a control was added, and Figure 1(b) shows the morphology of epidermal cells when an extract according to the present invention (extract of Production Example 3) was added as a sample solution. As shown in Figure 1, it was observed that epidermal cells cultured in the presence of the extract according to the present invention (extract of Production Example 3) were significantly flattened (Figure 1(b)). This morphological change is similar to the change that occurs when epidermal cells transform into keratinocytes in vivo, suggesting that the extract according to the present invention (extract of Production Example 3) activates epidermal cells, resulting in the formation of robust keratinocytes.

[0053] As described above, the fermented product of the present invention and the extract of the present invention have the effect of promoting epidermal cell proliferation, intracellular acidification, and flattening of epidermal cells. Therefore, by combining the fermented product of the present invention with the extract of the present invention, a synergistic effect of epidermal cell activation can be expected, which suggests the effect of maintaining normal skin turnover and epidermal thickness, and of leading to a strong and healthy barrier function.

[0054] Below, examples of formulations for skin external preparations containing the fermented products and extracts according to the present invention, such as lotions, emulsions, and creams, are shown, but the present invention is not limited to these.

[0055] Prescription example 1. Lotion [Ingredients] Part Jojoba oil 1.0 Fermented product of Production Example 1 0.7 Glycerin 5.0 Niacinamide 5.0 Dipotassium glycyrrhizinate 0.1 1,3-butylene glycol 1.0 Water-soluble collagen 0.01 Propanediol 3.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0056] Prescription example 2: Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 0.2 parts of the extract from Production Example 2 was used instead of the fermented product from Production Example 1 contained in Formulation Example 1.

[0057] Prescription example 3: Lotion A lotion was obtained in the same manner as in Formulation Example 1, except that 0.10 parts of the extract of Production Example 3 was used instead of the fermented product of Production Example 1 contained in Formulation Example 1.

[0058] Prescription example 4: Lotion [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Squalane 1.0 Glycerin 5.0 Niacinamide 5.0 Tranexamic acid 2.0 1,3-butylene glycol 1.0 Hydrolyzed Collagen 0.1 Pentanediol 3.0 Sodium citrate (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0059] Prescription example 5. Lotion [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Hydrogenated castor oil 1.0 Glycerin 5.0 L-Ascorbic Acid 2-Glucosyl 2.0 Tocopheryl acetate 0.3 Water-soluble collagen 0.01 1,3-butylene glycol 1.0 Pentanediol 3.0 Potassium hydroxide (appropriate amount) Purified water (enough to make the total volume 100 parts)

[0060] Prescription example 6. Emulsion [Ingredients] Part Fermented product of Production Example 1 0.7 Squalane 5.0 Jojoba oil 1.0 Orange Peel Oil 1.0 Polyoxyethylene (20) Sorbitan Monostearate 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Niacinamide 5.0 D-Pantothenyl alcohol 0.1 Glycerin 3.0 Carboxymethylcellulose 0.3 Xanthan gum 0.2 Sodium hyaluronate 0.01 1,3-butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water (enough to make the total volume 100 parts)

[0061] Prescription example 7. Emulsion An emulsion was obtained in the same manner as in Formulation Example 6, except that 0.2 parts of the extract from Preparation Example 2 was used instead of the fermented product from Preparation Example 1 contained in Formulation Example 6.

[0062] Prescription example 8. Emulsion An emulsion was obtained in the same manner as in Formulation Example 6, except that 0.1 parts of the extract from Preparation Example 3 was used instead of the fermented product from Preparation Example 1 contained in Formulation Example 6.

[0063] Prescription example 9. Emulsion [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Squalane 5.0 Castor oil 1.0 Lipophilic glyceryl stearate 1.0 Hydrogenated soy lecithin 1.5 Niacinamide 5.0 Tranexamic acid 2.0 Glycerin 3.0 Carboxymethylcellulose 0.3 Sodium hyaluronate 0.01 Hydrolyzed Hyaluronic Acid 0.01 1,3-butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water (enough to make the total volume 100 parts)

[0064] Prescription example 10. Cream [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Olive oil 5.0 Jojoba oil 5.0 Squalane 5.0 Niacinamide 5.0 Tranexamic acid 2.0 Dipotassium glycyrrhizinate 0.1 Lactic acid bacteria fermented rice 2.0 Hydrogenated lecithin 0.5 Carboxyvinyl polymer 0.3 Sodium alginate 0.2 Water-soluble collagen 0.1 1,3-butylene glycol 0.1 Phenoxyethanol 0.5 Pentylene glycol 0.5 Diglycerin 0.3 Purified water (enough to make the total volume 100 parts)

[0065] Prescription example 7. Facial cleanser [Ingredients] Part Fermented product of Production Example 1 0.7 Extract of Preparation Example 2 0.2 Extract of Preparation Example 3 0.1 Dipotassium glycyrrhizinate 0.1 Glyceryl Stearate 2.5 Squalane 3.0 Glycerin 3.0 Polyglyceryl-10 Laurate 3.0 Water-soluble collagen 0.01 Sodium hyaluronate 0.01 Isopropylmethylphenol 0.1 1,3-butylene glycol 2.0 Purified water (enough to make the total volume 100 parts)

Claims

[Claim 1] An epidermal cell activator comprising at least one of a fermented product of Job's tears seeds, a Salicornia herb extract, and an Aspalathus linearis extract.

Images