Vitamin b12 and bottled beverage containing carnosine

Abstract

To provide a novel food and drink containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitecobalamin.SOLUTION: According to one aspect of the present invention, a bottled beverage is provided containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitecobalamin, wherein the content of carnosine is 0.70 to 15 mg / mL, and the content of the at least one cobalamin is 0.005 to 1 μg / mL.SELECTED DRAWING: None

Description

[Technical Field]

[0001] The present invention is directed to the development of vitamin B 12 The present invention relates to a packaged beverage containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, and carnosine, and a method for producing the same. The present invention also relates to a method for stabilizing cobalamin and a method for improving the aftertaste of carnosine. The present invention also relates to an agent for inhibiting the degradation of cobalamin. [Background technology]

[0002] In recent years, with the rise in health consciousness, foods and beverages containing various nutritional ingredients are being manufactured and sold. For example, there are foods and beverages containing various vitamins and minerals. There are various vitamins added to foods and beverages, and common examples include vitamin A, vitamin B group, vitamin C, vitamin E, and vitamin K. Vitamin B group includes vitamin B1, vitamin B2, vitamin B6, vitamin B 12 , niacin, pantothenic acid, folic acid and biotin are commonly used.

[0003] Each vitamin has a known nutritional function. According to the website of the Consumer Affairs Agency of Japan, vitamin A is listed as a nutrient that helps maintain healthy skin and mucous membranes, vitamin B1 is listed as a nutrient that helps produce energy from carbohydrates and maintain healthy skin and mucous membranes, and vitamin B 12 is described as a nutrient that aids in the formation of red blood cells.

[0004] On the other hand, some vitamins are easily decomposed, and much research is being done to stabilize these vitamins in food and drink. For example, vitamin B 12 can be destabilized by heat or other ingredients in foods and beverages, accelerating their decomposition.

[0005] Japanese Patent Application Laid-Open No. 2019-110827 (Patent Document 1) describes the use of vitamin B in an acidic composition. 12 However, it has been discovered that the presence of ethanol increases the decomposition rate and / or decreases the stability of vitamin B. 12 In Japanese Patent Laid-Open Publication No. 2022-162843 (Patent Document 2), an acidic composition containing vitamin B 12 It has been described that vitamin B decomposes in an acidic aqueous solution, and a concentrate for a water-soluble vitamin drink has been proposed, which contains (1) a water-soluble vitamin, (2) one or more selected from polyoxyethylene hydrogenated castor oil and polyoxyethylene castor oil, (3) one or more selected from the group consisting of polyglycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene glycerin fatty acid ester, sucrose fatty acid ester, and polyoxyethylene alkyl ether, and (4) a polyhydric alcohol. 12 is significantly lost by heating, and is essentially free of glucose, amino acids, vitamin B1, ascorbyl palmitate, and / or vitamin C. 12 Formulations have been proposed. [Prior art documents] [Patent documents]

[0006] [Patent Document 1] Japanese Patent Application Publication No. 2019-110827 [Patent Document 2] Japanese Patent Publication No. 2022-162843 [Patent Document 3] Special Publication No. 2023-542821 Summary of the Invention [Problem to be solved by the invention]

[0007] Under the circumstances described above, there is a need for the development of new foods and beverages containing at least one type of cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. [Means for solving the problem]

[0008] The present inventors have found that adding a predetermined amount of carnosine to a packaged beverage containing a predetermined amount of cobalamin can suppress the decomposition of cobalamin. Furthermore, the present inventors have found that cobalamin can improve the undesirable taste of carnosine. The present invention is based on these findings.

[0009] The present invention includes, for example, the following aspects. [1] Carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin; A packaged beverage comprising: The carnosine content is 0.70 to 15 mg / mL. A packaged beverage, wherein the content of the at least one type of cobalamin is 0.005 to 1 μg / mL. [2] A packaged beverage according to [1], containing chicken extract. [3] The packaged beverage according to [1] or [2], wherein the at least one cobalamin comprises cyanocobalamin. [4] The packaged beverage according to any one of [1] to [3], wherein the carnosine content is 0.75 to 7 mg / mL. [5] The packaged beverage according to any one of [1] to [4], wherein the carnosine content is 0.80 to 4 mg / mL. [6] The packaged beverage according to any one of [1] to [5], wherein the content of the at least one kind of cobalamin is 0.04 to 0.4 μg / mL. [7] The packaged beverage according to any one of [1] to [6], wherein the weight ratio of the content of carnosine to the content of the at least one kind of cobalamin is 500:1 to 50,000:1. [8] The packaged beverage according to any one of [1] to [7], wherein after a retort test at 120°C for 8 minutes, 80% by weight or more of the at least one type of cobalamin is maintained relative to the content of the at least one type of cobalamin before the retort test. [9] The packaged beverage according to any one of [1] to [8], which is a ready-to-drink (RTD) beverage, a soft drink, a chicken soup, a jelly-like beverage, or a functional beverage.

[10] [1] - [9] The method for producing a bottled beverage according to any one of the above, adding at least one of said at least one cobalamin and carnosine to a beverage base.

[11] 1. A method for stabilizing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, comprising: The method further comprises adding carnosine to the solution containing at least one type of cobalamin at a concentration of 0.01 to 15 mg / mL.

[12] A method for improving the aftertaste of carnosine, comprising: A solution containing 0.01 to 15 mg / mL of carnosine was The method comprises containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin at a concentration of 0.005 to 1 μg / mL. [3] Carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin; A packaged beverage comprising: The carnosine content is 0.50 to 15 mg / mL, The content of the at least one cobalamin is 0.005 to 1 μg / mL, and A packaged beverage containing less than 0.3 mg / mL of propylene glycol.

[14] The packaged beverage according to any one of [1] to [9] and

[13] , wherein the ethanol content is less than 0.001 mg / mL.

[15] An agent for inhibiting the degradation of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin and sulfitocobalamin, which comprises carnosine. [Effects of the Invention]

[0010] According to one aspect of the present invention, a packaged beverage can be provided in which the stability of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin is improved. According to another aspect of the present invention, a packaged beverage can be provided in which the undesirable taste of carnosine is improved and which contains at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. [Brief explanation of the drawings]

[0011] [Figure 1] FIG. 1 is a graph showing the retention rate (%) of cyanocobalamin contained in each experimental sample, with the amount of cyanocobalamin contained in the control sample (not retort-heated) set at 100. [Figure 2]Figure 2 shows the mean percent retention of carnosine at various concentrations based on data from experimental samples containing 0.005 μg / mL, 0.04 μg / mL, 0.05 μg / mL, 0.1 μg / mL, and 0.4 μg / mL of cyanocobalamin. DETAILED DESCRIPTION OF THE INVENTION

[0012] The present invention will be described in detail below. The following embodiments are examples for explaining the present invention, and are not intended to limit the present invention to these embodiments. The present invention can be implemented in various forms without departing from the spirit of the present invention. Any paragraph headings used in this specification are for organizational purposes only and should not be construed as limiting the subject matter described. All documents cited in this specification, as well as published patent applications, patent publications and other patent documents, are hereby incorporated by reference.

[0013] 1. Cobalamin and carnosine drinks In one aspect, the present invention provides a beverage containing cobalamin and carnosine (hereinafter also referred to as the "beverage of the present invention"). According to a first aspect of the present invention, Carnosine and A packaged beverage containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.70 to 15 mg / mL. The present invention provides a packaged beverage in which the content of the at least one type of cobalamin is 0.005 to 1 μg / mL.

[0014] According to a second aspect of the present invention, Carnosine and A packaged beverage containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.50 to 15 mg / mL, The content of the at least one cobalamin is 0.005 to 1 μg / mL, A packaged beverage is provided that contains less than 0.3 mg / mL of propylene glycol.

[0015] Specific embodiments of the first and second aspects of the present invention will be described below, but unless otherwise specified, the following embodiments apply to both the first and second aspects.

[0016] The present inventors have surprisingly found that the stability of cobalamin can be improved by adjusting the content of cobalamin and the content of carnosine within a specific range.Without wishing to be bound by theory, in the basic structure of cobalamin, cobalt ions are coordinated to the dimethylbenzimidazole moiety. This dimethylbenzimidazole can be originally replaced with histidine having an imidazole moiety, and it is presumed that carnosine, which also contains an imidazole moiety, chelates the cobalt ions to form a stable carnosine-cobalt complex.As demonstrated in the examples below, the stability of cyanocobalamin (a type of cobalamin) tends to improve with an increase in the concentration of carnosine, but on the other hand, it has been observed that the stability of cyanocobalamin tends to decrease with an increase in the concentration of carnosine. Although we do not wish to be bound by theory on this point either, it is speculated that when the concentration of carnosine becomes too high, the coordination of dimethylbenzimidazole is lost, resulting in an unstable structure of cobalamin.

[0017] Beverages according to preferred embodiments of the present invention can inhibit the decomposition of the at least one cobalamin due to high-temperature heat sterilization treatment such as a retort test. After a retort test at 120°C for 8 minutes, a beverage according to one embodiment of the present invention maintains more than 70% by weight of the at least one cobalamin, relative to the content of the at least one cobalamin before the retort test. After a retort test at 120°C for 8 minutes, a beverage according to preferred embodiments of the present invention maintains 75% by weight or more, 80% by weight or more, 85% by weight or more, 90% by weight or more, 92% by weight or more, 94% by weight or more, 96% by weight or more, 98% by weight or more, or approximately 100% by weight of the at least one cobalamin, relative to the content of the at least one cobalamin before the retort test.

[0018] The inventors have also unexpectedly found that the undesirable taste of carnosine can be suppressed by adjusting the content of carnosine and at least one of the above cobalamins within a specific range.The mechanism of human taste is complex, and it was completely unexpected that the taste of carnosine could be improved by the presence of a very small amount of at least one of the above cobalamins.

[0019] <Cobalamin> Cobalamin is a B vitamin 12 Cobalamin is a general term for a group of vitamins classified as water-soluble. Cobalamin is a complex of cobalt. The most commonly used cobalamin is cyanocobalamin, whose molecular formula is C 63 H 88 CoN 14 O 14 P. Its CAS number is 68-19-9. Cobalamins other than cyanocobalamin include hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. Hydroxocobalamin is a cobalamin that has a hydroxyl group instead of the cyano group of cyanocobalamin, and its molecular formula is C. 62 H 89 CoN 13 O 15P and its CAS number is 13422-51-0. Adenosylcobalamin is a cobalamin that has an adenosyl group instead of the cyano group of cyanocobalamin, and its molecular formula is C 72 H 100 CoN 18 O 17 P and its CAS number is 13870-90-1. Methylcobalamin is a cobalamin with a methyl group instead of the cyano group of cyanocobalamin, and its molecular formula is C 63 H 91 CoN 13 O 14 P and its CAS number is 13422-55-4. Sulfitocobalamin is a cobalamin that has a sulfite group instead of the cyano group of cyanocobalamin, and its molecular formula is C 62 H 89 CoN 13 O 17 It is a lactic acid bacteria, and its CAS number is 15671-27-9. A beverage according to one embodiment of the present invention comprises at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. In one embodiment of the present invention, the at least one cobalamin preferably comprises cyanocobalamin and / or hydroxocobalamin, and more preferably comprises cyanocobalamin.

[0020] The origin of the at least one cobalamin used in the present invention is not particularly limited. Therefore, in the present invention, chemically synthesized cobalamin, cobalamin biosynthesized from a bacterial culture medium, or cobalamin isolated from a natural source containing cobalamin may be used. Alternatively, cobalamin may be added to the beverage by adding a food material containing cobalamin. Examples of food materials rich in cobalamin include seafood (especially clams and short-necked clams) and animal meat (e.g., chicken liver).

[0021] In the beverage according to one aspect of the present invention, the content of at least one of the above cobalamins is 0.005 to 1 μg / mL. The content of at least one of the above cobalamins is, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.005 to 0.8 μg / mL, 0.01 to 0.8 μg / mL, 0.02 to 0.۸ μg / mL, 0.03 to 0.8 μg / mL, 0.04 to 0.8 μg / mL, 0.05 to 0.8 μg / mL, 0.06 to 0.8 μg / mL, 0.07 to 0.8 μg / mL, 0.08 to 0.۸ μg / mL, 0.09 to 0.8 μg / mL, 0.1 to 0.8 μg / mL, 0.۱۵ to ۸ μg / mL, 0.2 to 0.8 μg / mL, 0.25 to 0.8 μg / mL, 0.3 to 0.8 μg / mL, 0.35 to 0.8 μg / mL, 0.4 to 0.8 μg / mL, 0.005 to ۞.6 μg / mL, 0.01 to 0.6 μg / mL, 0.02 to 0.6 μg / mL, 0.03 to 0.6 μg / mL, 0.04 to 0.6 μg / mL, 0.05 to 0.6 μg / mL, 0.06 to 0.6 μg / mL, 0.07 to ۰.۶ μg / mL, 0.08 to 0.6 μg / mL, 0.09 to 0.6 μg / mL, 0.1 to 0.6 μg / mL, 0.15 to 0.6 μg / mL, 0.2 to 0.6 μg / mL, 0.25 to 0.6 μg / mL, 0.3 to 0.6 μg / mL, 0.35 to 0.6 μg / mL, 0.4 to 0.6 μg / mL, 0.005 to 0.5 μg / mL, 0.01 to 0.5 μg / mL, 0.02 to 0.5 μg / mL, 0.03 to 0.5 μg / mL, 0.04 to 0.5 μg / mL, 0.05 to 0.5 μg / mL, 0.06 to 0.5 μg / mL, 0.07 to 0.5 μg / mL, 0.08 to 0.5 μg / mL, 0.09 to 0.5 μg / mL, 0.1 to 0.5 μg / mL, 0.15 to 0.5 μg / mL, 0.2 to 0.5 μg / mL, 0.25 to 0.5 μg / mL, 0.3 to 0.5 μg / mL, 0.35 to 0.5 μg / mL, 0.4 to 0.5 μg / mL, ۞.005 to ۞.4 μg / mL, 0.01 to ۞.4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07 ~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25 ~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0.2μg / mL, 0. 03~0.2μg / mL, 0.04~0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0.2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0.1μg / mL, 0.03~0.1μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0.1μ The content of the at least one cobalamin may be 0.02 to 0.4 μg / mL, 0.09 to 0.1 μg / mL, 0.005 to 0.09 μg / mL, 0.01 to 0.09 μg / mL, 0.02 to 0.09 μg / mL, 0.03 to 0.09 μg / mL, 0.04 to 0.09 μg / mL, 0.05 to 0.09 μg / mL, 0.06 to 0.09 μg / mL, 0.07 to 0.09 μg / mL, or 0.08 to 0.09 μg / mL. The content of the at least one cobalamin is preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL. When the beverage contains only one type of cobalamin, this content refers to the content of the cobalamin contained therein. When the beverage contains two or more types of cobalamin, this content refers to the total content.

[0022] The content of cyanocobalamin in the beverage according to one aspect of the present invention may be 0.005 to 1 μg / mL, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to l μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.005 to 0.8 μg / mL, 0.01 to 0.8 μg / mL, 0.02 to 0.8 μg / mL, 0.03 to 0.8 μg / mL, 0.04 to 0.8 μg / mL, 0.05 to 0.8 μg / mL, 0.06 to 0.8 μg / mL, 0.07 to 0.8 μg / mL, 0.08 to 0.8 μg / mL, 0.09 to 0.8 μg / mL, 0.1 to 0.8 μg / mL, 0.15 to 0.8 μg / mL, 0.2 to 0.8 μg / mL, 0.25 to 0.8 μg / mL, 0.3 to 0.8 μg / mL, 0.35 to 0.8 μg / mL, 0.4 to 0.8 μg / mL, 0.005 to 0.6 μg / mL, 0.01 to 0.6 μg / mL, 0.02 to 0.6 μg / mL, 0.03 to 0.6 μg / mL, 0.04 to 0.6 μg / mL, 0.05 to 0.6 μg / mL, 0.06 to 0.6 μg / mL, 0.07 to 0.6 μg / mL, '0.08 to 0.6 μg / mL, 0.09 to 0.6 μg / mL, 0.1 to 0.6 μg / mL, 0.15 to 0.6 μg / mL, 0.2 to 0.6 μg / mL, 0.25 to 0.6 μg / mL, 0.3 to 0.6 μg / mL, 0.35 to 0.6 μg / mL, 0.4 to 0.6 μg / mL, 0.005 to 0.5 μg / mL, 0.01 to 0.5 μg / mL, 0.02 to 0.5 μg / mL, 0.03 to 0.5 μg / mL, 0.04 to 0.5 μg / mL, 0.05 to 0.5 μg / mL, 0.06 to 0.5 μg / mL, 0.07 to 0.5 μg / mL, 0.08 to 0.5 μg / mL, 0.09 to 0.5 μg / mL, 0.1 to 0.5 μg / mL, 0.15 to 0.5 μg / mL, 0.2 to 0.5 μg / mL, 0.25 to 0.5 μg / mL, 0.3 to 0.5 μg / mL, 0.35 to 0.5 μg / mL, 0.4 to 0.5 μg / mL, 0.005 to 0.4 μg / mL, 0.01 to 0.4 μg / mL, 0.02 to 0.4 μg / mL, 0.03 to 0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07~0.4μg / mL, 0.08~0.4μg / mL, 0 .09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25~0.4μg / mL, 0.3~0.4μg / m L, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0.2μg / mL, 0.03~0.2μg / mL, 0.04~ 0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0.2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0.1μg / mL, 0.03~0.1 μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0.1μg / mL, 0. The cyanocobalamin content may be 0.09 to 0.1 μg / mL, 0.005 to 0.09 μg / mL, 0.01 to 0.09 μg / mL, 0.02 to 0.09 μg / mL, 0.03 to 0.09 μg / mL, 0.04 to 0.09 μg / mL, 0.05 to 0.09 μg / mL, 0.06 to 0.09 μg / mL, 0.07 to 0.09 μg / mL, or 0.08 to 0.09 μg / mL. The cyanocobalamin content is preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL.

[0023] The content of at least one cobalamin can be determined by liquid chromatography-mass spectrometry (LC-MS / MS). However, if the content can be calculated from the amount of cobalamin, it can also be determined from the amount of cobalamin. The measurement conditions for LC-MS / MS can be determined according to the standard measurement method of the analyzer. For LC-MS / MS, an Agilent 1290 Binary LC equipped with an AB Sciex 5600 and a TripleTOF system can be used. For example, a CORTECS T3 Column, 20 Å, 1.6 μm, 2.1 mm × 100 mm (manufactured by Waters Corporation) can be used as the LC column.

[0024] The packaged beverage according to one embodiment of the present invention contains cyanocobalamin and at least one vitamin selected from the group consisting of hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin (hereinafter referred to as "other vitamin B"). 12 Other vitamin B 12 If vitamins are included, the content of cyanocobalamin and other B vitamins 12 The composition ratio of the total amount of vitamin B stearate, vitamin B stearate, and vitamin B stearate may be 99:1 to 90:10, 99:1 to 91:9, 99:1 to 92:8, 99:1 to 93:7, 99:1 to 94:6, 99:1 to 95:5, 99:1 to 96:4, 99:1 to 97:3, or 99:1 to 98:2 by mass. 12 Vitamin B 12 As used herein, the term "consisting essentially of" refers to the vitamin B serotonin used in the present invention. 12 Vitamin B other than cyanocobalamin 12 This means that it may contain small amounts of vitamins such as vitamin B 12 If the product essentially consists of cyanocobalamin, the content of cyanocobalamin is less than that of vitamin B 12 This means that the content of cyanocobalamin is preferably 85 to 100% by weight, more preferably 90 to 100% by weight, and even more preferably 95 to 100% by weight, based on the total weight of the ingredients.12 100% by weight of the total weight of the group means that vitamin B 12 The group consists of cyanocobalamin and any other vitamin B 12 This means that the content of these substances is an impurity amount (less than 1% by weight).

[0025] Vitamin B 12 Cobalamin is known to have health benefits such as hematopoietic and nerve repair effects, so including cobalamin can be expected to improve eye fatigue, stiff shoulders, and neuralgia.

[0026] <Carnosine> In one embodiment of the present invention, the beverage contains carnosine. Carnosine is a type of imidazole peptide, and imidazole dipeptide is a general term for dipeptides whose constituent components are histidine and imidazole rings (Yanagiuchi, Nobuya, Journal of the Japanese Society of Food Chemical Technology, 2014, Vol. 61, No. 1, p. 45). Here, imidazole is a type of five-membered aromatic heterocyclic compound, a nitrogen-containing aromatic heterocyclic compound having nitrogen atoms at the first and third positions.

[0027] In one embodiment of the present invention, the carnosine used in the beverage may be obtained by extraction with an aqueous solvent from a carnosine-containing material (e.g., fish meat) or by chemical synthesis, and its origin and raw materials are not particularly limited. Alternatively, carnosine may be added to the beverage by adding a food ingredient containing carnosine. For example, carnosine is abundant in fish meat such as tuna, bonito, and salmon, as well as beef, pork, and chicken. Mammals such as pigs and cows contain a large amount of carnosine, while aquatic mammals contain carnosine and balenine in a ratio of about 1:2. In birds, carnosine and anserine are present in a ratio of 1:3. Large fish contain almost exclusively anserine (Yanagiuchi, Nobuya, Journal of the Japanese Society of Food and Chemical Technology, 2014, Vol. 61, No. 1, p. 45). In a preferred embodiment of the present invention, chicken extract, described below, can be used as a food ingredient containing carnosine.

[0028] The content of carnosine contained in the beverage in one embodiment of the first aspect of the present invention is 0.70 to 15 mg / mL, for example, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7 to 15 mg / mL, 7.5 to 15 mg / mL, 8 to 15 mg / mL, 0.70 to 12 mg / mL, 0.75 ~12mg / mL, 0.80~12mg / mL, 0.90~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12 mg / mL, 5~12mg / mL, 5.5~12mg / mL, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8~12mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90~10mg / mL, 1~10mg / mL, 1.5~10mg / mL, 2~10mg / mL, 2.5~10mg / mL, 3~10mg / mL, 3.5~10mg / mL, 4~10mg / mL, 4.5~10mg / mL, 5~10mg / mL, 5.5~10mg / mL, 6~10mg / mL, 6.5~10mg / mL, 7~10mg / mL, 7.5~10mg / mL, 8~10mg / mL, 0.70~8mg / mL, 0.75~8mg / mL, 0.80~8mg / mL, 0.90~8mg / mL, 1~8mg / mL, 1.5~8mg / mL, 2~8mg / mL, 2.5~8mg / mL, 3~8mg / mL, 3.5~8mg / mL, 4~8mg / mL, 4.5~8mg / mL, 5~8mg / mL, 5.5~8mg / mL, 6~8mg / mL, 6.5~8mg / mL, 7~8mg / mL, 7.5~8mg / mL, 0.70~7mg / mL, 0.75~7mg / mL, 0.80~7mg / mL, 0.90~7mg / mL, 1~7mg / mL, 1.5~7mg / mL, 2~7mg / mL, 2.5~7mg / mL, 3~7mg / mL, 3.5~7mg / mL, 4~7mg / mL, 4.5~7mg / mL, 5~7mg / mL, 5.5~7mg / mL, 6~7mg / mL, 6.5~7mg / mL, 0.70~6mg / mL, 0.75~6mg / mL, 0.80~6mg / mL, 0.90~6mg / mL, 1~6mg / mL, 1.5~6mg / mL, 2~6mg / mL, 2.5~6mg / mL, 3~6mg / mL, 3.5~6mg / mL, 4~6mg / mL, 4.5~6mg / mL, 5~6mg / mL, 5.5~6mg / mL, 0.70~4mg / mL, 0.75~4mg / mL, 0.80 The carnosine content may be 0.75 to 7 mg / mL, more preferably 0.80 to 4 mg / mL, 0.90 to 4 mg / mL, 1 to 4 mg / mL, 1.5 to 4 mg / mL, 2 to 4 mg / mL, 2.5 to 4 mg / mL, 3 to 4 mg / mL, 3.5 to 4 mg / mL, 0.70 to 3 mg / mL, 0.75 to 3 mg / mL, 0.80 to 3 mg / mL, 0.90 to 3 mg / mL, 1 to 3 mg / mL, 1.5 to 3 mg / mL, 2 to 3 mg / mL, 2.5 to 3 mg / mL, 0.70 to 2 mg / mL, 0.75 to 2 mg / mL, 0.80 to 2 mg / mL, 0.90 to 2 mg / mL, 1 to 2 mg / mL, or 1.5 to 2 mg / mL.

[0029] According to one embodiment of the second aspect of the present invention, the carnosine content is 0.50 to 15 mg / mL, for example, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7 to 15 mg / mL, 7.5 to 15 mg / mL, 8 to 15 mg / mL, g / mL, 0.50~12mg / mL, 0.60~12mg / mL, 0.70~12mg / mL, 0.75~12mg / mL, 0.80~12mg / mL, 0.90~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~ 12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12mg / mL, 5.5~12mg / mL, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8 ~12mg / mL, 0.50~10mg / mL, 0.60~10mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90~10mg / mL, 1~10mg / mL, 1.5~10mg / mL, 2~10mg / mL, 2.5~10mg / mL, 3~10mg / mL, 3.5~10mg / mL, 4~10mg / mL, 4.5~10mg / mL, 5~10mg / mL, 5.5~10mg / mL, 6~10mg / mL, 6.5~10mg / mL, 7~10mg / mL, 7.5~10mg / mL, 8~10mg / mL, 0.50~8mg / mL, 0.60~8mg / mL, 0.70~8mg / mL, 0.75~8mg / mL, 0.80~8mg / mL, 0.90~8mg / mL, 1~8mg / mL, 1.5~8mg / mL, 2~8mg / mL, 2.5~8 mg / mL, 3~8mg / mL, 3.5~8mg / mL, 4~8mg / mL, 4.5~8mg / mL, 5~8mg / mL, 5.5~8mg / mL, 6~8mg / mL, 6.5~8mg / mL, 7~8mg / mL, 7.5~8mg / mL, 0.50~7mg / mL, 0.60~7mg / mL, 0.70~7mg / mL, 0.75~7mg / mL, 0.80~7mg / mL, 0.90~7mg / mL, 1~7mg / mL, 1.5~7mg / mL, 2~7mg / mL , 2.5~7mg / mL, 3~7mg / mL, 3.5~7mg / mL, 4~7mg / mL, 4.5~7mg / mL, 5~7mg / mL, 5.5~7mg / mL, 6~7mg / mL, 6.5~7m g / mL, 0.50~6mg / mL, 0.60~6mg / mL, 0.70~6mg / mL, 0.75~6mg / mL, 0.80~6mg / mL, 0.90~6mg / mL, 1~6mg / mL, 1 .5~6mg / mL, 2~6mg / mL, 2.5~6mg / mL, 3~6mg / mL, 3.5~6mg / mL, 4~6mg / mL, 4.5~6mg / mL, 5~6mg / mL, 5.5~6mg / mL, 0.50~4mg / mL, 0.60~4mg / mL, 0.70~4mg / mL, 0.75~4mg / mL, 0.80~4mg / mL, 0.90~4mg / mL, 1~4mg / mL, 1.5 ~4mg / mL, 2~4mg / mL, 2.5~4mg / mL, 3~4mg / mL, 3.5~4mg / mL, 0.50~3mg / mL, 0.60~3mg / mL, 0.70~3mg / mL, 0.7 The carnosine concentration may be 5-3 mg / mL, 0.80-3 mg / mL, 0.90-3 mg / mL, 1-3 mg / mL, 1.5-3 mg / mL, 2-3 mg / mL, 2.5-3 mg / mL, 0.50-2 mg / mL, 0.60-2 mg / mL, 0.70-2 mg / mL, 0.75-2 mg / mL, 0.80-2 mg / mL, 0.90-2 mg / mL, 1-2 mg / mL, or 1.5-2 mg / mL. The carnosine content is preferably 0.75-7 mg / mL, more preferably 0.80-4 mg / mL.

[0030] The carnosine content can be determined by high-performance liquid chromatography (HPLC), but if the content can be calculated from the compounded amount, it can also be determined from the compounded amount. The measurement conditions for HPLC can be the standard measurement method of the analyzer. When measuring by HPLC, an ion exchange column (e.g., 300SCX (dimensions: 4.6 × 250 mm, particle size: 5 μm)) can be used.

[0031] The ratio of carnosine to the at least one cobalamin is not particularly limited, but in a preferred embodiment of the present invention, the weight ratio of the carnosine content to the at least one cobalamin content is 500:1 to 50,000:1. In another embodiment of the present invention, the weight ratio of the carnosine content to the at least one cobalamin content may be 1,000:1 to 50,000:1, 2,000:1 to 50,000:1, 4,000:1 to 50,000:1, 5,000:1 to 50,000:1, 8,000:1 to 50,000:1, 8,000:1 to 40,000:1, 8,000:1 to 35,000:1, or 8,000:1 to 30,000:1. Furthermore, from the viewpoint of improving the undesirable taste of carnosine, the weight ratio of the content of carnosine to the content of at least one of the above-mentioned cobalamins is preferably 4000:1 to 15000:1, more preferably 6000:1 to 12000:1, and even more preferably 6500:1 to 10000:1.

[0032] <Chicken extract> A beverage according to one embodiment of the present invention contains chicken extract, which is a component obtained by hot water extraction or the like using chicken meat, bones, skin, and other parts as raw materials. Chicken extract generally contains carnosine.

[0033] When the chicken extract used in the beverage of one embodiment of the present invention contains a carnosine salt, the content of carnosine in the chicken extract means the total amount of carnosine and its salt.

[0034] Examples of carnosine salts include, but are not limited to, inorganic salts such as hydroxides, carbonates, and bicarbonates of sodium, lithium, calcium, magnesium, and aluminum and ammonia; and organic salts such as mono-, di-, or tri-alkylamine salts, such as methylamine, dimethylamine, and trimethylamine salts, mono-, di-, or tri-hydroxyalkylamine salts, guanidine salts, and N-methylglucosamine salts.

[0035] When a chicken extract is used in a beverage according to one embodiment of the present invention, an extract obtained by heating raw materials such as chicken meat in a liquid may be used, or a commercially available chicken extract may be used. Raw materials for the chicken extract may include chicken bones, cartilage, feet (also known as maple), etc.

[0036] Commercially available chicken extracts include "Brand's Essence of Chicken (BEC)" (manufactured by Suntory Beverage & Food Asia Co., Ltd.), "Scotch® Essence of Chicken" (manufactured by Scotch Industrial Co., Ltd., Thailand), "Quaker Essence of chicken" (manufactured by Standard Foods Corporation, Taiwan), "SWANSON® Chicken stock and broth" (manufactured by Campbell Soup Company, Ltd.), "Drip Chicken Essence" (manufactured by Eu Yan Sang International Co., Ltd., Singapore), "Boned Chicken Tonic" (manufactured by Eu Yan Sang International Co., Ltd., Singapore), and "Boiled Essence of Chicken" (manufactured by Lao Xie Zhen Co., Ltd., Taiwan). While any of these commercially available products can be used, it is preferable to use Brand's Essence of Chicken (BEC).

[0037] When the chicken extract used in the beverage of one embodiment of the present invention is produced by hot water extraction of chicken meat, chicken bones, cartilage, feet, etc. (hereinafter referred to as "chicken meat, etc."), it can be produced by a commonly used method. For example, chicken extract can be produced by treating chicken meat, etc. in a solvent at high temperature under normal or increased pressure. When producing chicken extract, fat and cholesterol may be removed after crude extraction with a solvent, and then excess solvent may be removed to increase the concentration.

[0038] As used herein, chicken extract includes the liquid extract, diluted solution, concentrate, or dry powder obtained by the above-mentioned method, as well as purified products thereof. The purified product can be obtained, for example, by subjecting the chicken extract to diatomaceous earth filtration, ultrafiltration, membrane treatment, liquid separation, or fractionation using resins to achieve high purification.

[0039] The amount of chicken extract (solid content equivalent) in the beverage according to one embodiment of the present invention is not particularly limited, but is preferably 5% to 20% by weight. In preferred embodiments of the present invention, the amount of chicken extract (solid content equivalent) may be 6% to 14%, 8% to 12%, or 9% to 11% by weight.

[0040] <Drink Form> The beverage of the present invention is a packaged beverage. The type of container for the beverage is not limited, and the beverage may be packaged in a container such as a can, bottle, PET bottle, pouch, paper carton, or plastic container. According to a preferred embodiment of the present invention, the beverage is packaged in a small-portion pouch for convenient, regular consumption. If heat sterilization is performed after packaging, the type is not particularly limited, and can be carried out using conventional techniques such as inversion sterilization, UHT sterilization, and retort sterilization. The temperature for the heat sterilization step is not particularly limited, and is, for example, 65 to 130°C, preferably 80 to 120°C, for 1 to 40 minutes. However, as long as a sterilization value equivalent to that under the above conditions is obtained, sterilization at an appropriate temperature for a few seconds, for example, 5 to 30 seconds, is acceptable.

[0041] In one embodiment of the present invention, beverages include ready-to-drink (RTD) beverages, soft drinks, soups containing meat extracts (extracts obtained from chicken, beef, pork, etc.), such as chicken soup, jelly-like beverages, powdered beverages, and functional beverages. As used herein, powdered beverages refer to powdered beverages that are diluted with water or hot water to a predetermined concentration before consumption. Beverages containing chicken extract also include compositions that are heated and consumed in a soup-like state, such as pouched soups (e.g., chicken essence or DEOC (Drip Essence of Chicken)). In a preferred embodiment of the present invention, the packaged beverage is a ready-to-drink (RTD) beverage.

[0042] <Propylene glycol> A beverage according to one embodiment of the present invention contains propylene glycol, while a beverage according to another embodiment of the present invention does not contain propylene glycol. When propylene glycol is contained, the content may be, for example, less than 1 mg / mL, less than 0.8 mg / mL, less than 0.6 mg / mL, less than 0.4 mg / mL, less than 0.3 mg / mL, or less than 0.2 mg / mL. A beverage according to a second embodiment of the present invention contains carnosine and at least one of the above cobalamins, and has a propylene glycol content of less than 0.3 mg / mL. The propylene glycol content is not particularly limited as long as it is less than 0.3 mg / mL, and may be 0 mg / mL (or less than the lower limit of measurement), but may also be, for example, 0.15 mg / mL or less, 0.12 mg / mL or less, 0.10 mg / mL or less, or 0.08 mg / mL or less. Preferably, the propylene glycol content is 0 to 0.08 mg / mL. The amount of propylene glycol can be adjusted by selecting the ingredients to be added. For example, the amount of propylene glycol can be reduced by not adding fragrances, and the propylene glycol content can be measured by HS-SPME-GC-MS (headspace solid-phase microextraction gas chromatography-mass spectrometry).

[0043] <Other ingredients and properties> As described in Patent Document 1, since ethanol promotes the decomposition of cobalamin, it is preferable to limit its content. The ethanol content is preferably less than 0.001 mg / mL, and may be less than 0.0008 mg / mL or less than 0.0005 mg / mL. The amount of ethanol can be adjusted by selecting the raw materials to be added. For example, the amount of ethanol can be reduced by not adding flavorings. The ethanol content can be measured by HS-SPME-GC-MS.

[0044] A beverage according to one embodiment of the present invention is substantially free of one or both of copper and vitamin C. As used herein, "substantially free of copper" means that the iron content is less than 1 mM, and "substantially free of vitamin C" means that the vitamin C content is less than 10 mM. According to one embodiment of the present invention, the copper content in the beverage is less than 0.1 mM, and the vitamin C content is less than 1 mM.

[0045] A beverage according to one embodiment of the present invention contains caramel. A beverage according to another embodiment of the present invention does not contain melon juice. A beverage according to yet another embodiment of the present invention does not contain niacinamide, or has a niacinamide content of less than 26 mg / 100 ml, less than 20 mg / 100 ml, or less than 15 mg / 100 ml. A beverage according to yet another embodiment of the present invention does not contain vitamin B2 (riboflavin), or has a vitamin B2 content of less than 3.0 mg / 100 ml, less than 2.0 mg / 100 ml, or less than 1.5 mg / 100 ml. A beverage according to yet another embodiment of the present invention does not contain pyridoxine hydrochloride, or has a pyridoxine hydrochloride content of less than 5.0 mg / 100 ml, less than 4.0 mg / 100 ml, or less than 2.0 mg / 100 ml.

[0046] The beverage according to one embodiment of the present invention is a low-calorie beverage. Here, a low-calorie beverage refers to, for example, a beverage with a calorie content of 20 kcal / bottle or less, preferably 18 kcal / bottle or less, and more preferably 16 kcal / bottle or less. The calorie content of a beverage can be calculated by calculating the content of each ingredient contained in the beverage and multiplying the calculated value by an energy conversion factor, or by measuring the physical heat of combustion using a calorie meter (e.g., a bomb calorie meter) and correcting the calculated value for digestion and absorption rate, excreted heat, etc.

[0047] The beverage according to one embodiment of the present invention contains water. The water is not particularly limited, and any type of water can be used as long as it does not adversely affect the flavor, including tap water, ion-exchanged water, softened water, distilled water, carbonated water, reverse osmosis water (RO water), activated carbon-treated water, purified water, and demineralized water.

[0048] The pH of the beverage according to one embodiment of the present invention may preferably be 4 to 7, 4.5 to 7, 5 to 7, 5.5 to 7, 6 to 7, 4 to 6.8, 4.5 to 6.8, 5 to 6.8, 5.5 to 6.8, 6 to 6.8, 4 to 6.6, 4.5 to 6.6, 5 to 6.6, 5.5 to 6.6, or 6 to 6.6. Adjusting the pH within this range can achieve a pleasant taste with less sourness.

[0049] The Brix (soluble solids content) of the beverage according to one embodiment of the present invention is preferably 5 to 20, more preferably 6 to 14, and even more preferably 8 to 12. The Brix (soluble solids content) of the beverage can be measured using a sugar content refractometer.

[0050] The beverage according to one embodiment of the present invention may contain a sweetener. The sweetener may be a low-intensity sweetener, a high-intensity sweetener, or a combination thereof. Examples of low-intensity sweeteners include glucose, sucrose (e.g., granulated sugar), fructose, maltose, oligosaccharides, high-fructose corn syrup, lactose, psicose, allose, tagatose, xylose, and ribose. Examples of high-intensity sweeteners include aspartame, neotame, advantame, sucralose, acesulfame potassium (acesulfame K), saccharin, saccharin sodium, sodium cyclamate, dulcin, disodium glycyrrhizinate, trisodium glycyrrhizinate, neohesperidin dihydrochalcone, thaumatin, monellin, curculin, mabinlin, brazzein, pentadine, hernandulcin, 4β-hydroxyhernandulcin, miraculin, glycyrrhizin, rubusoside, phyllodulcin, mogroside IV, mogroside V, steviol glycosides (rebaudioside A, rebaudioside D, rebaudioside M), thaumatin, monellin, brazzein, monatin, monk fruit extract, and stevia extract.

[0051] The beverage according to one embodiment of the present invention may contain, in addition to the above-mentioned components, components that can be added to ordinary beverages, as long as the effects of the present invention are not impaired. Examples of such components include acidulants, flavorings, vitamins, colorants, antioxidants, emulsifiers, preservatives, seasonings, extracts, pH adjusters, and quality stabilizers.

[0052] [Examples of beverages of the present invention] Illustrative embodiments of the beverage of the present invention are shown below, but the present invention is not limited to the following embodiments. According to one aspect of the present invention, carnosine and vitamin B 12 A packaged beverage comprising: The content of carnosine is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL; The present invention provides a packaged beverage containing 0.005 to 1 μg / mL of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. According to one aspect of the present invention, carnosine and vitamin B 12 A packaged beverage comprising: The content of carnosine is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL; A packaged beverage is provided having a cyanocobalamin content of 0.005 to 1 μg / mL.

[0053] According to one aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The content of carnosine is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL; The present invention provides a packaged beverage in which the content of the at least one type of cobalamin is 0.04 to 0.4 μg / mL. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, The content of carnosine is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL; A packaged beverage is provided having a cyanocobalamin content of 0.04 to 0.4 μg / mL.

[0054] According to one aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, Contains chicken extract, The content of carnosine is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL; The present invention provides a packaged beverage in which the content of the at least one type of cobalamin is 0.005 to 1 μg / mL. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, Contains chicken extract, The content of carnosine is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL; A packaged beverage is provided having a cyanocobalamin content of 0.005 to 1 μg / mL.

[0055] According to one aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, Contains chicken extract, The carnosine content is 0.75 to 7 mg / mL. The present invention provides a packaged beverage in which the content of the at least one type of cobalamin is 0.005 to 1 μg / mL, preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL, or 0.005 to 0.09 μg / mL. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, Contains chicken extract, The carnosine content is 0.75 to 7 mg / mL. The present invention provides a packaged beverage having a cyanocobalamin content of 0.005 to 1 μg / mL, preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL, or 0.005 to 0.09 μg / mL.

[0056] According to one aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.50 to 15 mg / mL, The content of the at least one cobalamin is 0.005 to 1 μg / mL, and A packaged beverage is provided having a propylene glycol content of less than 0.3 mg / mL, preferably 0.15 mg / mL or less or 0.08 mg / mL or less. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, The carnosine content is 0.50 to 15 mg / mL, The cyanocobalamin content is 0.005 to 1 μg / mL, and A packaged beverage is provided having a propylene glycol content of less than 0.3 mg / mL, preferably 0.15 mg / mL or less or 0.08 mg / mL or less.

[0057] According to one aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, The carnosine content is 0.50 to 15 mg / mL, The content of the at least one cobalamin is 0.005 to 1 μg / mL, and The propylene glycol content is 0.15 mg / mL or less, The following requirements: (i) Does not contain melon juice; (ii) does not contain niacinamide or contains less than 26 mg / 100 ml of niacinamide; (iii) does not contain vitamin B2 or contains less than 3.0 mg / 100 ml of vitamin B2; and (iv) Does not contain pyridoxine hydrochloride or contains less than 5.0 mg / 100 ml of pyridoxine hydrochloride A packaged beverage that satisfies one or more of the above is provided. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, The carnosine content is 0.50 to 15 mg / mL, The cyanocobalamin content is 0.005 to 1 μg / mL, and The propylene glycol content is 0.15 mg / mL or less, The following requirements: (i) Does not contain melon juice; (ii) does not contain niacinamide or contains less than 26 mg / 100 ml of niacinamide; (iii) does not contain vitamin B2 or contains less than 3.0 mg / 100 ml of vitamin B2; and (iv) Does not contain pyridoxine hydrochloride or contains less than 5.0 mg / 100 ml of pyridoxine hydrochloride A packaged beverage that satisfies one or more of the above is provided.

[0058] 2. Beverage manufacturing method As one aspect, the present invention provides a method for producing a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL and the at least one cobalamin content is 0.005 to 1 μg / mL (hereinafter also referred to as "the method for producing the beverage of the present invention").

[0059] A method for producing a beverage according to one embodiment of the present invention includes adding at least one of the above-described cobalamin and carnosine to a beverage base. A method for producing a beverage according to a preferred embodiment of the present invention includes adding water to the carnosine of the present invention and the above-described at least one cobalamin to obtain a packaged beverage having a carnosine content of 0.70 to 15 mg / mL and a cobalamin content of 0.005 to 1 μg / mL.

[0060] In one embodiment of the present invention, a beverage manufacturing method can add a predetermined amount of carnosine to a beverage by adding ingredients rich in carnosine to the beverage. Regarding ingredients rich in carnosine, those listed in the section "1. Beverages containing cobalamin and carnosine" can be used as appropriate.

[0061] As used herein, the term "beverage base" refers to a liquid that serves as a precursor to a beverage, and is prepared by adding predetermined ingredients to the beverage base and optionally subjecting it to predetermined processing, heat sterilization, etc. Examples of beverage bases include, but are not limited to, water, carbonated water, fruit juice, soup containing meat extract (extract obtained from chicken, beef, pork, etc.), such as chicken soup, sports drinks, or combinations thereof.

[0062] The production method according to one embodiment of the present invention may include a packaging step and a subsequent sterilization step. Packing can be carried out by any known method. When heat sterilization is carried out after packaging, the type of heat sterilization is not particularly limited, and can be carried out using conventional techniques such as UHT sterilization and retort sterilization. The temperature in the heat sterilization step is not particularly limited, but is, for example, 50 to 130°C, preferably 50 to 120°C, for 10 to 40 minutes. However, as long as a sterilization value equivalent to that under the above conditions is obtained, sterilization at an appropriate temperature for a few seconds, for example, 5 to 30 seconds, is also acceptable.

[0063] In the method for producing the beverage of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other ingredients" described in the section "1. Beverages containing cobalamin and carnosine" can be used. The form and characteristics of the beverage can also be determined based on the descriptions in the section "1. Beverages containing cobalamin and carnosine."

[0064] 3. Stabilization method of cobalamin In one aspect, the present invention provides the following method for stabilizing cobalamin (hereinafter also referred to as the "stabilization method of the present invention"). According to one aspect of the present invention, there is provided a method for stabilizing at least one type of cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, in which carnosine is added to a solution containing the at least one type of cobalamin so as to have a concentration of 0.01 to 15 mg / mL.

[0065] The present inventors have surprisingly found that the stability of cobalamin can be improved by adding carnosine to a solution containing cobalamin at a predetermined concentration. For example, the stabilization method according to one embodiment of the present invention can suppress the decomposition of cobalamin during heat treatment such as retort sterilization.

[0066] In one embodiment of the stabilization method of the present invention, the concentration of carnosine in the solution containing at least one type of cobalamin is 0.01 to 15 mg / mL. In another embodiment, the concentration of carnosine is 0.05 to 15 mg / mL, 0.10 to 15 mg / mL, 0.20 to 15 mg / mL, 0.30 to 15 mg / mL, 0.40 to 15 mg / mL, 0.50 to 15 mg / mL, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 5 to 15 mg / mL, 6 to 15 mg / mL, 7 to 15 mg / mL, 8 to 15 mg / mL, 9 to 15 mg / mL, 10 to 15 mg / mL, 11 to 15 mg / mL, 12 to 15 mg / mL, 13 to 15 mg / mL, 14 to 15 mg / mL, 15 to 15 mg / mL, 16 to 16 mg / mL, 17 to 17 mg / mL, 18 to 18 mg / mL, 19 to 20 mg / mL, 21 to 22 mg / mL, 22 to 23 mg / mL, 23 to 24 mg / mL, 24 to 25 mg / mL, 25 to 26 mg / mL, 26 to 27 mg / mL, 27 to 15mg / mL, 4.5~15mg / mL, 5~15mg / mL, 5.5~15mg / mL, 6~15mg / mL, 6.5~15mg / mL, 7~15mg / mL, 7.5~15mg / mL, 8~15mg / mL, 0.01~12mg / mL, 0.05~1 2mg / mL, 0.10~12mg / mL, 0.20~12mg / mL, 0.30~12mg / mL, 0.40~12mg / mL, 0.50~12mg / mL, 0.60~12mg / mL, 0.70~12mg / mL, 0.75~12mg / mL, 0.80~ 12mg / mL, 0.90~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12mg / mL, 5.5~12mg / mL, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8~12mg / mL, 0.01~10mg / mL, 0.05~10mg / mL, 0.10~10mg / mL, 0.20~10 mg / mL, 0.30~10mg / mL, 0.40~10mg / mL, 0.50~10mg / mL, 0.60~10mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90~10mg / mL, 1~10mg / mL, 1.5~10mg / mL, 2~10mg / mL, 2.5~10mg / mL, 3~10mg / mL, 3.5~10mg / mL, 4~10mg / mL, 4.5~10mg / mL, 5~10mg / mL, 5.5~10mg / mL, 6~10mg / mL, 6.5~10mg / mL、7~10mg / mL、7.5~10mg / mL、8~10mg / mL、0.01~8mg / mL、0.05~8mg / mL、0.10~8mg / mL、0.20~8mg / mL、0.30~8mg / mL、0.40~8mg / mL、0.50~8mg / mL、0.60~8mg / mL、0.70~8mg / mL、0.75~8mg / mL、0.80~8mg / mL、0.90~8mg / mL、1~8mg / mL、1.5~8mg / mL、2~8mg / mL、2.5~8mg / mL、3~8mg / mL、3.5~8mg / mL、4~8mg / mL、4.5~8mg / mL、5~8mg / mL、5.5~8mg / mL、6~8mg / mL、6.5~8mg / mL、7~8mg / mL、7.5~8mg / mL、0.01~7mg / mL、0.05~7mg / mL、0.10~7mg / mL、0.20~7mg / mL、0.30~7mg / mL、0.40~7mg / mL、0.50~7mg / mL、0.60~7mg / mL、0.70~7mg / mL、0.75~7mg / mL、0.80~7mg / mL、0.90~7mg / mL、1~7mg / mL、1.5~7mg / mL、2~7mg / mL、2.5~7mg / mL、3~7mg / mL、3.5~7mg / mL、4~7mg / mL、4.5~7mg / mL、5~7mg / mL、5.5~7mg / mL、6~7mg / mL、6.5~7mg / mL、0.01~6mg / mL、0.05~6mg / mL、0.10~6mg / mL、0.20~6mg / mL、0.30~6mg / mL、0.40~6mg / mL、0.50~6mg / mL、0.60~6mg / mL、0.70~6mg / mL、0.75~6mg / mL、0.80~6mg / mL、0.90~6mg / mL、1~6mg / mL、1.5~6mg / mL、2~6mg / mL、2.5~6mg / mL、3~6mg / mL、3.5~6mg / mL、4~6mg / mL、4.5~6mg / mL、5~6mg / mL、5.5~6mg / mL、6~6mg / mL、6.5~6mg / mL、7~6mg / mL、7.5~6mg / mL、8~6mg / mL、0.01~4mg / mL、0.05~4mg / mL、0.10~4mg / mL、0.20~4mg / mL、0.30~4mg / mL、0.40~4mg / mL、0.50~4mg / mL、0.60~4mg / mL、0.70~4mg / mL、0.75~4mg / mL, 0.80~4mg / mL, 0.90~4mg / mL, 1~4mg / mL, 1.5~4mg / mL, 2~4mg / mL, 2.5~4mg / mL, 3~4mg / mL, 3.5~4mg / mL, 0.01~3mg / mL, 0.05~3 mg / mL, 0.10~3mg / mL, 0.20~3mg / mL, 0.30~3mg / mL, 0.40~3mg / mL, 0.50~3mg / mL, 0.60~3mg / mL, 0.70~3mg / mL, 0.75~3mg / mL, 0.80~3mg / mL The carnosine concentration may be 0.90 to 3 mg / mL, 1 to 3 mg / mL, 1.5 to 3 mg / mL, 2 to 3 mg / mL, 2.5 to 3 mg / mL, 0.01 to 2 mg / mL, 0.05 to 2 mg / mL, 0.10 to 2 mg / mL, 0.20 to 2 mg / mL, 0.30 to 2 mg / mL, 0.40 to 2 mg / mL, 0.50 to 2 mg / mL, 0.60 to 2 mg / mL, 0.70 to 2 mg / mL, 0.75 to 2 mg / mL, 0.80 to 2 mg / mL, 0.90 to 2 mg / mL, 1 to 2 mg / mL, or 1.5 to 2 mg / mL. Preferably, the carnosine concentration may be 0.75 to 7 mg / mL or 0.80 to 4 mg / mL. A stabilization method according to one embodiment of the present invention includes adding carnosine to achieve the above concentrations.

[0067] When adding carnosine to a solution containing at least one of the above-mentioned cobalamins, it may be added by extracting carnosine from a material containing carnosine (e.g., fish or chicken) with an aqueous solvent, or by chemical synthesis. Alternatively, carnosine may be added by adding food ingredients containing carnosine to the beverage.

[0068] In the stabilization method according to one aspect of the present invention, the concentration of the at least one cobalamin in the solution containing the at least one cobalamin is not particularly limited, and may be, for example, 0.005 to 1 μg / mL, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, / mL, 0.3-1μg / mL, 0.35-1μg / mL, 0.4-1μg / mL, 0.005-0.8μg / mL, 0.01-0.8μg / mL, 0.02-0.8μg / mL, 0.03-0.8μg / mL, 0.04-0.8μg / mL, 0.05-0.8μg / mL, 0.06-0.8μg / mL, 0.07-0.8μg / mL, 0.08-0.8μg / mL, 0.09-0.8μg / mL, 0.1-0.8μg / mL, 0.15-0.8μg / mL, 0.2-0.8μg / mL, 0.25-0.8μg / mL, 0.3-0.8μg / mL, 0.35 ~0.8μg / mL, 0.4~0.8μg / mL, 0.005~0.6μg / mL, 0.01~0.6μg / mL, 0.02~0.6μg / mL, 0.03~0.6μg / mL, 0.04~0.6μg / mL, 0.05~0.6μg / mL, 0.06~0.6μg / mL, 0.0 7~0.6μg / mL, 0.08~0.6μg / mL, 0.09~0.6μg / mL, 0.1~0.6μg / mL, 0.15~0.6μg / mL, 0.2~0.6μg / mL, 0.25~0.6μg / mL, 0.3~0.6μg / mL, 0.35~0.6μg / mL, 0.4~0 .6μg / mL, 0.005~0.5μg / mL, 0.01~0.5μg / mL, 0.02~0.5μg / mL, 0.03~0.5μg / mL, 0.04~0.5μg / mL, 0.05~0.5μg / mL, 0.06~0.5μg / mL, 0.07~0.5μg / mL, 0.08 ~0.5μg / mL, 0.09~0.5μg / mL, 0.1~0.5μg / mL, 0.15~0.5μg / mL, 0.2~0.5μg / mL , 0.25~0.5μg / mL, 0.3~0.5μg / mL, 0.35~0.5μg / mL, 0.4~0.5μg / mL, 0.005~0.4μg / mL, 0.01~0.4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0 .4μg / mL, 0.07~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4 μg / mL, 0.25~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0. 2μg / mL, 0.03~0.2μg / mL, 0.04~0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0. 2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0 .1μg / mL, 0.03~0.1μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0 The concentration of the at least one cobalamin may be 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL. When the beverage contains only one type of cobalamin, this concentration refers to the concentration of the cobalamin contained therein. When the beverage contains two or more types of cobalamin, this concentration refers to the total concentration of the cobalamins contained therein.

[0069] In the stabilization method according to one aspect of the present invention, the concentration of cyanocobalamin in the solution containing cyanocobalamin is not particularly limited, and may be, for example, 0.005 to 1 μg / mL, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35~1μg / mL, 0.4~1μg / mL, 0.005~0.8μg / mL, 0.01~0.8μg / mL, 0.02~0.8μg / mL, 0.03~0.8μg / mL, 0.04~0.8μg / mL, 0.05~0.8μg / mL, 0.06~0.8μg / mL, 0.0 7~0.8μg / mL, 0.08~0.8μg / mL, 0.09~0.8μg / mL, 0.1~0.8μg / mL, 0.15~0.8μg / mL, 0.2~0.8μg / mL, 0.25~0.8μg / mL, 0.3~0.8μg / mL, 0.35~0.8μg / mL, 0.4~0 .8μg / mL, 0.005~0.6μg / mL, 0.01~0.6μg / mL, 0.02~0.6μg / mL, 0.03~0.6μg / mL, 0.04~0.6μg / mL, 0.05~0.6μg / mL, 0.06~0.6μg / mL, 0.07~0.6μg / mL, 0.08 ~0.6μg / mL, 0.09~0.6μg / mL, 0.1~0.6μg / mL, 0.15~0.6μg / mL, 0.2~0.6μg / mL, 0.25~0.6μg / mL, 0.3~0.6μg / mL, 0.35~0.6μg / mL, 0.4~0.6μg / mL, 0.005~0 .5μg / mL, 0.01~0.5μg / mL, 0.02~0.5μg / mL, 0.03~0.5μg / mL, 0.04~0.5μg / m L, 0.05~0.5μg / mL, 0.06~0.5μg / mL, 0.07~0.5μg / mL, 0.08~0.5μg / mL, 0.09~ 0.5μg / mL, 0.1~0.5μg / mL, 0.15~0.5μg / mL, 0.2~0.5μg / mL, 0.25~0.5μg / mL , 0.3~0.5μg / mL, 0.35~0.5μg / mL, 0.4~0.5μg / mL, 0.005~0.4μg / mL, 0.01~0.4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07 ~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25 ~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0.4μg / mL, 0.005~0.2μg / mL, 0.01~0.2μg / mL, 0.02~0.2μg / mL, 0. 03~0.2μg / mL, 0.04~0.2μg / mL, 0.05~0.2μg / mL, 0.06~0.2μg / mL, 0.07~0.2μg / mL, 0.08~0.2μg / mL, 0.09~0.2μg / mL, 0.1~0.2μg / mL, 0.15~0.2μg / mL, 0.005~0.1μg / mL, 0.01~0.1μg / mL, 0.02~0.1μg / mL, 0.03~0.1μg / mL, 0.04~0.1μg / mL, 0.05~0.1μg / mL, 0.06~0.1μg / mL, 0.07~0.1μg / mL, 0.08~0.1μ The concentration of cyanocobalamin may be 0.02 to 0.1 μg / mL, 0.005 to 0.09 μg / mL, 0.01 to 0.09 μg / mL, 0.02 to 0.09 μg / mL, 0.03 to 0.09 μg / mL, 0.04 to 0.09 μg / mL, 0.05 to 0.09 μg / mL, 0.06 to 0.09 μg / mL, 0.07 to 0.09 μg / mL, or 0.08 to 0.09 μg / mL. The concentration of cyanocobalamin is preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL.

[0070] In the stabilization method of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other ingredients" described in the section "1. Beverages containing cobalamin and carnosine" can be used.

[0071] 4. How to improve the aftertaste of carnosine As one aspect, the present invention provides the following method for improving the aftertaste of carnosine (hereinafter also referred to as the "aftertaste improving method of the present invention"). According to one aspect of the present invention, there is provided a method for improving the aftertaste of carnosine, which comprises adding at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin to a solution containing 0.01 to 15 mg / mL of carnosine, to give a concentration of 0.005 to 1 μg / mL.

[0072] The present inventors have surprisingly found that the undesirable taste of carnosine can be suppressed by adjusting the content of carnosine and the at least one cobalamin within a specific range.

[0073] In one embodiment of the aftertaste improving method of the present invention, the concentration of carnosine is 0.01 to 15 mg / mL. According to another embodiment, the concentration of carnosine is 0.05 to 15 mg / mL, 0.10 to 15 mg / mL, 0.20 to 15 mg / mL, 0.30 to 15 mg / mL, 0.40 to 15 mg / mL, 0.50 to 15 mg / mL, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, mL, 4.5~15mg / mL, 5~15mg / mL, 5.5~15mg / mL, 6~15mg / mL, 6.5~15mg / mL, 7~15mg / mL, 7.5~15mg / mL, 8~15mg / mL, 0.01~12mg / mL, 0.05~12mg / mL, 0 .10~12mg / mL, 0.20~12mg / mL, 0.30~12mg / mL, 0.40~12mg / mL, 0.50~12mg / mL, 0.60~12mg / mL, 0.70~12mg / mL, 0.75~12mg / mL, 0.80~12mg / mL, 0.9 0~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12mg / mL, 5.5~12mg / m L, 6~12mg / mL, 6.5~12mg / mL, 7~12mg / mL, 7.5~12mg / mL, 8~12mg / mL, 0.01~10mg / mL, 0.05~10mg / mL, 0.10~10mg / mL, 0.20~10mg / mL, 0.30~10mg / m L, 0.40~10mg / mL, 0.50~10mg / mL, 0.60~10mg / mL, 0.70~10mg / mL, 0.75~10mg / mL, 0.80~10mg / mL, 0.90~10mg / mL, 1~10mg / mL, 1.5~10mg / mL, 2~1 0mg / mL, 2.5~10mg / mL, 3~10mg / mL, 3.5~10mg / mL, 4~10mg / mL, 4.5~10mg / mL, 5~10mg / mL, 5.5~10mg / mL, 6~10mg / mL, 6.5~10mg / mL, 7~10mg / mL, 7.5~10mg / mL、8~10mg / mL、0.01~8mg / mL、0.05~8mg / mL、0.10~8mg / mL、0.20~8mg / mL、0.30~8mg / mL、0.40~8mg / mL、0.50~8mg / mL、0.60~8mg / mL、0.70~8mg / mL、0.75~8mg / mL、0.80~8mg / mL、0.90~8mg / mL、1~8mg / mL、1.5~8mg / mL、2~8mg / mL、2.5~8mg / mL、3~8mg / mL、3.5~8mg / mL、4~8mg / mL、4.5~8mg / mL、5~8mg / mL、5.5~8mg / mL、6~8mg / mL、6.5~8mg / mL、7~8mg / mL、7.5~8mg / mL、0.01~7mg / mL、0.05~7mg / mL、0.10~7mg / mL、0.20~7mg / mL、0.30~7mg / mL、0.40~7mg / mL、0.50~7mg / mL、0.60~7mg / mL、0.70~7mg / mL、0.75~7mg / mL、0.80~7mg / mL、0.90~7mg / mL、1~7mg / mL、1.5~7mg / mL、2~7mg / mL、2.5~7mg / mL、3~7mg / mL、3.5~7mg / mL、4~7mg / mL、4.5~7mg / mL、5~7mg / mL、5.5~7mg / mL、6~7mg / mL、6.5~7mg / mL、0.01~6mg / mL、0.05~6mg / mL、0.10~6mg / mL、0.20~6mg / mL、0.30~6mg / mL、0.40~6mg / mL、0.50~6mg / mL、0.60~6mg / mL、0.70~6mg / mL、0.75~6mg / mL、0.80~6mg / mL、0.90~6mg / mL、1~6mg / mL、1.5~6mg / mL、2~6mg / mL、2.5~6mg / mL、3~6mg / mL、3.5~6mg / mL、4~6mg / mL、4.5~6mg / mL、5~6mg / mL、5.5~6mg / mL、6~6mg / mL、6.5~6mg / mL、7~6mg / mL、7.5~6mg / mL、8~6mg / mL、0.01~4mg / mL、0.05~4mg / mL、0.10~4mg / mL、0.20~4mg / mL、0.30~4mg / mL、0.40~4mg / mL、0.50~4mg / mL、0.60~4mg / mL、0.70~4mg / mL、0.75~4mg / mL、0.80~4mg / mL、0.90~4mg / mL, 1~4mg / mL, 1.5~4mg / mL, 2~4mg / mL, 2.5~4mg / mL, 3~4mg / mL, 3.5~4mg / mL, 0.01~3mg / mL, 0.05~3mg / mL, 0.10~3mg / mL, 0.20~3mg / mL, 0.30~3mg / mL, 0.40~3mg / mL, 0.50~3mg / mL, 0.60~3mg / mL, 0.70~3mg / mL, 0.75~3mg / mL, 0.80~3mg / mL, 0.90~3mg / mL The carnosine concentration may be 1 to 3 mg / mL, 1.5 to 3 mg / mL, 2 to 3 mg / mL, 2.5 to 3 mg / mL, 0.01 to 2 mg / mL, 0.05 to 2 mg / mL, 0.10 to 2 mg / mL, 0.20 to 2 mg / mL, 0.30 to 2 mg / mL, 0.40 to 2 mg / mL, 0.50 to 2 mg / mL, 0.60 to 2 mg / mL, 0.70 to 2 mg / mL, 0.75 to 2 mg / mL, 0.80 to 2 mg / mL, 0.90 to 2 mg / mL, 1 to 2 mg / mL, or 1.5 to 2 mg / mL. Preferably, the carnosine concentration may be 0.40 to 7 mg / mL or 0.50 to 4 mg / mL. A stabilization method according to one embodiment of the present invention includes adding carnosine to achieve the above concentrations.

[0074] When adding at least one type of cobalamin to a solution containing carnosine, the at least one type of cobalamin may be obtained by extraction with an aqueous solvent from a material containing the at least one type of cobalamin (for example, seafood (especially clams and short-necked clams) or animal meat (e.g., chicken liver)), or may be obtained by chemical synthesis. Alternatively, the at least one type of cobalamin may be added by adding a food ingredient containing the at least one type of cobalamin to the beverage.

[0075] In one embodiment of the aftertaste improving method of the present invention, the at least one cobalamin is contained at a concentration of 0.005 to 1 μg / mL. According to another embodiment, the at least one cobalamin is contained at a concentration of, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.005 ~0.8μg / mL, 0.01~0.8μg / mL, 0.02~0.8μg / mL, 0.03~0.8μg / mL, 0.04~0.8μg / mL, 0.05~0.8μg / mL, 0.06~0.8μg / mL, 0.07~0.8μg / mL, 0.08~0.8μ g / mL, 0.09~0.8μg / mL, 0.1~0.8μg / mL, 0.15~0.8μg / mL, 0.2~0.8μg / mL, 0.25~0.8μg / mL, 0.3~0.8μg / mL, 0.35~0.8μg / mL, 0.4~0.8μg / mL, 0.00 5~0.6μg / mL, 0.01~0.6μg / mL, 0.02~0.6μg / mL, 0.03~0.6μg / mL, 0.04~0.6μg / mL, 0.05~0.6μg / mL, 0.06~0.6μg / mL, 0.07~0.6μg / mL, 0.08~0.6 μg / mL, 0.09~0.6μg / mL, 0.1~0.6μg / mL, 0.15~0.6μg / mL, 0.2~0.6μg / mL, 0.25~0.6μg / mL, 0.3~0.6μg / mL, 0.35~0.6μg / mL, 0.4~0.6μg / mL, 0.0 05~0.5μg / mL, 0.01~0.5μg / mL, 0.02~0.5μg / mL, 0.03~0.5μg / mL, 0.04~0.5μg / mL, 0.05~0.5μg / mL, 0.06~0.5μg / mL, 0.07~0.5μg / mL, 0.08~0. 5μg / mL, 0.09~0.5μg / mL, 0.1~0.5μg / mL, 0.15~0.5μg / mL, 0.2~0.5μg / mL, 0.25~0.5μg / mL, 0.3~0.5μg / mL, 0.35~0.5μg / mL, 0.4~0.5μg / mL, 0.005~0.4μg / mL, 0.01~0.4μg / mL, 0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0. 07~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25~0.4μg / mL, 0.3~0 The concentration of the at least one cobalamin may be 0.02 to 0.8 μg / mL, more preferably 0.05 to 0.6 μg / mL, 0.005 to 0.2 μg / mL, 0.01 to 0.2 μg / mL, 0.02 to 0.2 μg / mL, 0.03 to 0.2 μg / mL, 0.04 to 0.2 μg / mL, 0.05 to 0.2 μg / mL, 0.06 to 0.2 μg / mL, 0.07 to 0.2 μg / mL, 0.08 to 0.2 μg / mL, 0.09 to 0.2 μg / mL, 0.1 to 0.2 μg / mL, or 0.15 to 0.2 μg / mL. This concentration refers to the concentration of the cobalamin contained in a beverage containing only one type of cobalamin, and refers to the combined concentration of the cobalamins contained in a beverage containing two or more types of cobalamin.

[0076] In one aspect of the present invention, the concentration of シノノココレラノン, which contains シアノココレラMIN, is not limited, for example, 0.005~1μg / mL, 0.01~1μg / mL, 0.02~1μg / mL, 0.03~1μg / mL, 0.04~1μg / mL, 0.04~1μg / mL. 05~1μg / mL、0.06~1μg / mL、0.07~1μg / mL、0.08~1μg / mL、0.09~1μg / mL、0.1~1μg / mL、0.15~1μg / mL、0.2~1μg / mL、0.25~1μg / mL、0.3~1μg / mL、0.35~1μg / mL L、0.4~1μg / mL、0.005~0.8μg / mL、0.01~0.8μg / mL、0.02~0.8μg / mL、0.03~0.8μg / mL、0.04~0.8μg / mL、0.05~0.8μg / mL、0.06~0.8μg / mL、0.07~0.8μg / mL 、0.08~0.8μg / mL、0.09~0.8μg / mL、0.1~0.8μg / mL、0.15~0.8μg / mL、0.2~0. 8μg / mL、0.25~0.8μg / mL、0.3~0.8μg / mL、0.35~0.8μg / mL、0.4~0.8μg / mL、0. 005~0.6μg / mL、0.01~0.6μg / mL、0.02~0.6μg / mL、0.03~0.6μg / mL、0.04~0.6μg / mL、0.05~0.6μg / mL、0.06~0.6μg / mL、0.07~0.6μg / mL、0.08~0.6μg / mL 、0.09~0.6μg / mL、0.1~0.6μg / mL、0.15~0.6μg / mL、0.2~0.6μg / mL、0.25~0. 6μg / mL、0.3~0.6μg / mL、0.35~0.6μg / mL、0.4~0.6μg / mL、0.005~0.5μg / mL、0 .01~0.5μg / mL、0.02~0.5μg / mL、0.03~0.5μg / mL、0.04~0.5μg / mL、0.05~0. 5μg / mL、0.06~0.5μg / mL、0.07~0.5μg / mL、0.08~0.5μg / mL、0.09~0.5μg / mL、 0.1~0.5μg / mL、0.15~0.5μg / mL、0.2~0.5μg / mL、0.25~0.5μg / mL、0.3~0.5μ g / mL、0.35~0.5μg / mL、0.4~0.5μg / mL、0.005~0.4μg / mL、0.01~0.4μg / mL、0.02~0.4μg / mL, 0.03~0.4μg / mL, 0.04~0.4μg / mL, 0.05~0.4μg / mL, 0.06~0.4μg / mL, 0.07~0.4μg / mL, 0.08~0.4μg / mL, 0.09~0.4μg / mL, 0.1~0.4μg / mL, 0.15~0.4μg / mL, 0.2~0.4μg / mL, 0.25~0.4μg / mL, 0.3~0.4μg / mL, 0.35~0. The concentration of cyanocobalamin may be 0.4 μg / mL, 0.005 to 0.2 μg / mL, 0.01 to 0.2 μg / mL, 0.02 to 0.2 μg / mL, 0.03 to 0.2 μg / mL, 0.04 to 0.2 μg / mL, 0.05 to 0.2 μg / mL, 0.06 to 0.2 μg / mL, 0.07 to 0.2 μg / mL, 0.08 to 0.2 μg / mL, 0.09 to 0.2 μg / mL, 0.1 to 0.2 μg / mL, or 0.15 to 0.2 μg / mL. The concentration of cyanocobalamin is preferably 0.02 to 0.8 μg / mL, more preferably 0.05 to 0.6 μg / mL.

[0077] In the aftertaste improving method of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other ingredients" may be those described in the section "1. Beverages containing cobalamin and carnosine."

[0078] 5. Cobalamin decomposition inhibitor In one aspect, the present invention provides a cobalamin degradation inhibitor (hereinafter also referred to as the "degradation inhibitor of the present invention"), which comprises carnosine and at least one cobalamin degradation inhibitor selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin.

[0079] The degradation inhibitor according to one embodiment of the present invention consists essentially of carnosine. This means that the degradation inhibitor according to one embodiment of the present invention may contain small amounts of components other than carnosine. For example, when the degradation inhibitor consists essentially of carnosine, the carnosine content is preferably 85 to 100 wt %, more preferably 90 to 100 wt %, and even more preferably 95 to 100 wt %, based on the total weight of the degradation inhibitor. A carnosine content of 100 wt % based on the total weight of the degradation inhibitor means that the degradation inhibitor is composed of carnosine, and the content of any other components is an impurity amount (less than 1 wt %).

[0080] The degradation inhibitor according to one embodiment of the present invention may contain, as other components, various components that can be orally ingested by humans, such as a solvent such as water, a bulking agent, or a filler.

[0081] The form of the decomposition inhibitor according to one embodiment of the present invention is not particularly limited, and may be either liquid or solid.

[0082] As used herein, the term "at least" means that the number of a particular item may be equal to or greater than the recited number. Also, as used herein, the term "about" means that the subject lies within a range of ±25%, ±10%, ±5%, ±3%, ±2%, or ±1% of the numerical value following "about." For example, "about 10" means a range of 7.5 to 12.5. [Example]

[0083] The present invention will be described in more detail below with reference to examples, but the present invention is not limited to the following examples.

[0084] Example 1-1: Evaluation of the stability of cyanocobalamin Carnosine (catalog no. 9625, Sigma-Aldrich USA, purity ≥ 99%) and cyanocobalamin (catalog no. 0429155, DSM Netherlands, purity ≥ 96%) were used to prepare 80 mg / mL carnosine standard solutions and 2 mg / mL cyanocobalamin standard solutions, respectively. These standard solutions were diluted to produce 10x stock solutions for experimental sample preparation.

[0085] For each experimental sample, 45 carnosine-cyanocobalamin-containing samples were prepared by mixing a 10x carnosine stock solution with a 10x cyanocobalamin stock solution and diluting appropriately with tap water. Similarly, samples with the same composition as samples 1, 10, 19, 28, and 37 were prepared as control samples (without retort sterilization). Note that the carnosine used in the test was 99% pure or higher. Therefore, assuming that all impurities were propylene glycol, a sample containing 8 mg / mL of carnosine would contain a maximum of approximately 0.08 mg / mL (approximately 80 ppm). Furthermore, since the cyanocobalamin was 96% pure or higher, a sample containing 0.4 μg / mL of cyanocobalamin would contain a maximum of approximately 0.016 μg / mL (approximately 0.016 ppm) of propylene glycol, assuming that all impurities were propylene glycol. [Table 1]

[0086] All experimental samples were subjected to heat treatment in an autoclave at 120°C for 8 minutes, conditions that mimic a retort sterilization process, and then cooled to ambient temperature for 2 hours before analysis.

[0087] The amount of cyanocobalamin contained in each heat-treated sample was determined by LC-MSMS under the following conditions. A known method was used for the measurement, and an Agilent 1290 Binary LC equipped with an AB Sciex 5600 and a triple TOF system was used. A CORTECS T3 column (120 Å, 1.6 μm, 2.1 mm × 100 mm (Waters)) was used as the liquid chromatograph column. Other conditions are as follows: Injected sample volume: 5 μL Mobile phase: Solution A: Water containing 0.1% formic acid Solution B: Acetonitrile containing 0.1% formic acid Column temperature: 40℃ Flow rate: 0.4mL / min Gradient (concentration of solution B) 0-1 min: Solution B concentration 1% 1 to 7.5 minutes: Solution B concentration 1% → 40% 7.5-10 min: Solution B concentration 40% → 100% 10-12.5 min: Solution B concentration 100% → 100% 12.5-13 min: Solution B concentration 100% → 1% 13-15 minutes: Solution B concentration 1% [Table 2]

[0088] The measurement results are shown in Figure 1 and Tables 3 and 4. Table 3 summarizes the actual cyanocobalamin content measured after the retort test in the presence of carnosine. Table 4 shows the percentage of cyanocobalamin contained in each experimental sample, with the amount of cyanocobalamin contained in the control sample (unheated) set at 100. Because the control sample was not subjected to heat treatment, it is assumed that the cyanocobalamin contained in the control sample was not reduced by thermal decomposition. Therefore, the percentage of cyanocobalamin contained in each experimental sample shown in Table 4 can be considered equivalent to the retention of cyanocobalamin after heat treatment. [Table 3]

[0089] [Table 4]

[0090] Furthermore, to verify the stabilizing effect of carnosine on cyanocobalamin, the obtained data are summarized in Figure 2. Figure 2 shows the average data for experimental samples containing cyanocobalamin at 0.005 μg / mL, 0.04 μg / mL, 0.05 μg / mL, 0.1 μg / mL, and 0.4 μg / mL of carnosine concentration.

[0091] Example 1-2: Evaluation of the stability of cyanocobalamin in a chicken extract-containing beverage A sample containing 0.110 μg / mL (11.0 μg / 100 mL) of cyanocobalamin and 0.77 mg / mL of carnosine was prepared using cyanocobalamin (catalog no. 0429155, manufactured by DSM Netherlands, purity ≥96%) and chicken extract (manufactured by Suntory Beverage and Food Thailand, carnosine content: 0.77 g / L). The resulting chicken soup beverage was subjected to heat treatment in the same manner as in Example 1-1. The cyanocobalamin concentrations in the chicken soup beverage before and after heat treatment were measured in the same manner as in Example 1-1. The cyanocobalamin concentration in the chicken soup beverage before heat treatment was 10.9 μg / 100 mL, and the cyanocobalamin concentration after heat treatment was 11.02 μg / 100 mL. This indicates that nearly 100% of the cyanocobalamin was retained before and after heat treatment.

[0092] Example 2: Sensory evaluation of carnosine Samples A to J were prepared by adding carnosine (catalog No. 9625, Sigma-Aldrich USA, purity 99% or higher) and cyanocobalamin (catalog No. 0429155, DSM Netherlands, purity 96% or higher) to water to obtain the compositions shown in Table 5. Control samples, which did not contain cyanocobalamin, were also prepared.

[0093] The taste of the sample beverages obtained using the above procedure was evaluated by four expert panelists based on the following criteria. [Sensory evaluation criteria] Scoring criteria 6. The aftertaste is greatly improved. 5. The aftertaste is greatly improved. 4. Improved aftertaste. 3. The aftertaste is slightly improved. 2. The aftertaste is slightly improved. 1. The aftertaste is slightly improved. 0 No difference from control sample.

[0094] Before conducting the test, the panelists thoroughly discussed the above evaluation criteria. The results are summarized in Table 5. The values ​​for the sensory evaluation results in the table below are the average scores of the four panelists. [Table 5]

Claims

1. Carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin; A packaged beverage comprising: The carnosine content is 0.70 to 15 mg / mL, A packaged beverage, wherein the content of the at least one cobalamin is 0.005 to 1 μg / mL.

2. The packaged beverage according to claim 1, which contains chicken extract.

3. 3. The packaged beverage according to claim 1 or 2, wherein the at least one cobalamin comprises cyanocobalamin.

4. The packaged beverage according to any one of claims 1 to 3, wherein the carnosine content is 0.75 to 7 mg / mL.

5. The packaged beverage according to any one of claims 1 to 4, wherein the carnosine content is 0.80 to 4 mg / mL.

6. The bottled beverage according to any one of claims 1 to 5, wherein the content of the at least one cobalamin is 0.04 to 0.4 µg / mL.

7. The bottled beverage according to any one of claims 1 to 6, wherein the weight ratio of the content of carnosine to the content of the at least one type of cobalamin is 500:1 to 50,000:

1.

8. 8. The bottled beverage according to claim 1, wherein after a retort test at 120°C for 8 minutes, 80% by weight or more of the at least one cobalamin is maintained relative to the content of the at least one cobalamin before the retort test.

9. The packaged beverage according to any one of claims 1 to 8, which is a ready-to-drink (RTD) beverage, a soft drink, a chicken soup, a jelly-like beverage or a functional beverage.

10. The method for producing the container-packed beverage according to any one of claims 1 to 9, adding at least one of said at least one cobalamin and carnosine to a beverage base.

11. 1. A method for stabilizing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, comprising: The method further comprises adding carnosine to the solution containing at least one cobalamin at a concentration of 0.01 to 15 mg / mL.

12. A method for improving the aftertaste of carnosine, comprising: A solution containing 0.01 to 15 mg / mL of carnosine The method of claim 1, further comprising containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin at a concentration of 0.005 to 1 μg / mL.

13. Carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin; A packaged beverage comprising: The carnosine content is 0.50 to 15 mg / mL, The content of the at least one cobalamin is 0.005 to 1 μg / mL; and A packaged beverage having a propylene glycol content of less than 0.3 mg / mL.

14. The packaged beverage according to any one of claims 1 to 9 and 13, wherein the ethanol content is less than 0.001 mg / mL.

15. An agent for inhibiting the degradation of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin and sulfitocobalamin, which comprises carnosine.

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