Skin whitening composition and skin whitening method using the same

Abstract

Provided is a skin whitening composition comprising a compound represented by the following formula 1 as an active ingredient, and a method for whitening the skin of an individual, comprising administering the composition to the individual: [Formula 1] TIFF2025509366000011.tif31155 (wherein each substituent in Formula 1 is as defined in the specification.)

Description

[Technical field]

[0001] This description relates to a skin whitening composition and a method for whitening the skin utilizing the same. [Background technology]

[0002] Skin performs various functions essential for the survival of the human body. The most representative functions of skin are a barrier function to maintain internal homeostasis in response to environmental changes, a sensory function to recognize external changes, and a thermoregulatory function. Among the various functions of skin, the barrier function of skin is mainly manifested by the stratum corneum located at the outermost part of the skin. It has been reported that the stratum corneum not only functions as a barrier but also affects the functions, roles, and structures of the internal living cell layers, i.e., the epidermis and dermis, and its importance is continuously increasing. The stratum corneum is composed of dead keratinocytes and intercellular lipids, and plays a key role as a skin protective film that protects the skin from external stimuli and prevents moisture from evaporating from the inside. In addition, keratinocytes in the stratum corneum create a skin barrier through differentiation and keratinization processes.

[0003] There are many factors that cause aging of human skin, but ultraviolet rays in particular cause wrinkles, loss of elasticity, pigmentation, and loss of moisture in the skin due to damage to the skin barrier. When moisture on the skin surface decreases due to damage to the skin caused by ultraviolet rays, the stratum corneum on the skin surface loses its flexibility and the skin becomes dry, and as a result, the skin cannot function as a barrier. Therefore, maintaining the skin's moisture is very important in order to strengthen the skin barrier.

[0004] However, as aging progresses, the skin barrier weakens, making it more difficult to maintain the skin's moisture content. Therefore, in recent years, active research has been conducted into methods for whitening the skin while enhancing the skin's defense power, regardless of the skin's moisture content. In particular, active research is being conducted into melanin-producing cells, which are closely related to skin pigmentation. [Prior art documents] [Patent documents]

[0005] [Patent Document 1] Korean Patent No. 1700967 Summary of the Invention [Problem to be solved by the invention]

[0006] An object of one embodiment is to provide a cosmetic composition for skin whitening.

[0007] An object of another embodiment is to provide a method for whitening the skin of an individual, comprising administering to the individual said cosmetic composition for whitening the skin. [Means for solving the problem]

[0008] According to one embodiment, there is provided a composition for whitening comprising a compound represented by the following Chemical Formula 1 as an active ingredient:

[0009] [ka]

[0010] In the above Chemical Formula 1, X is CRR' (R and R' are each independently a hydrogen atom or a substituted or unsubstituted C1-C10 alkyl group), an oxygen atom, or a sulfur atom; L 1 is a substituted or unsubstituted C1 to C20 alkylene group, R 1 is a hydroxy group, a substituted or unsubstituted C1 to C20 alkyl group, or a substituted or unsubstituted C1 to C20 alkoxy group.

[0011] In the formula 1, X is an oxygen atom, and R 1 can be a hydroxy group.

[0012] The formula 1 may be represented by the following formula 1-1.

[0013] [ka]

[0014] The compound represented by Chemical Formula 1 may be contained at a concentration of 0.01 μM to 10 mM based on the total amount of the composition.

[0015] The compound represented by Chemical Formula 1 may be derived from lactobacillus ferment.

[0016] The whitening composition may be a cosmetic composition.

[0017] According to another embodiment, there is provided a method for whitening the skin of an individual, comprising administering to the individual a cosmetic composition comprising a compound represented by the following Chemical Formula 1 as an active ingredient:

[0018] According to another embodiment, there is provided a use of the compound represented by Formula 1 for preparing a composition for whitening.

[0019] According to another embodiment, there is provided a method for whitening the skin, comprising applying to the skin a composition containing an effective amount of the compound represented by Chemical Formula 1.

[0020] The compound represented by Chemical Formula 1 and its concentration are as described above. Effect of the Invention

[0021] The composition according to one embodiment can achieve skin whitening effects by inhibiting melanin production in melanocytes in human skin without cytotoxicity, thereby suppressing skin pigmentation. [Brief description of the drawings]

[0022] [Figure 1]1 is a photograph showing that a composition according to an embodiment inhibits melanin production in normal human melanocytes (a photograph of a cell suspension in which cells treated with an experimental substance and cultured for 8 days were collected in an e-tube and dissolved in 1N NaOH solution). [Diagram 2] 1 is a graph showing that a composition according to an embodiment inhibits melanin production in normal human melanocytes. [Diagram 3] 1 is a graph showing that a composition according to one embodiment does not cause cytotoxicity. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0023] DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will now be described in detail with reference to the accompanying drawings, in which: FIG. 1 is a block diagram of a semiconductor device according to an embodiment of the present invention;

[0024] In this specification, improvement of skin whitening function means that the color of the stratum corneum located on the outside of the skin changes to lighter and whiter. The stratum corneum, which is the first defense membrane of the skin, is easily damaged by the external environment. In order to impart whitening power to the skin, a general prescription for applying a skin topical agent can solve the temporary problem, but it is difficult to solve the fundamental problem. Furthermore, most skin topical agents do not solve the problem, but only have the effect of hiding dark marks in the skin, so the problem of hyperpigmentation of the skin cannot be fundamentally improved by simply applying a skin topical agent. Therefore, the present inventors have confirmed that a compound represented by a specific chemical formula constituting a composition according to one embodiment can solve the fundamental problem and fundamentally improve the skin whitening effect, and have completed the present invention.

[0025] More specifically, the composition according to one embodiment is expected to have a skin whitening effect by inhibiting melanin synthesis in normal human melanocytes.

[0026] In relation to this, it has been shown that when the intracellular concentration of c-AMP increases, melanin production increases, and when the extracellular signal regulated kinase (ERK) pathway is activated, melanin production decreases. However, a well-known method for activating the extracellular signal regulated kinase pathway is to stimulate the signal transduction pathway that phosphorylates extracellular signal regulated kinase. Recently, it has been shown that when protein phosphatase 2A (PP2A), an enzyme that dephosphorylates extracellular signal regulated kinase, is inhibited, extracellular signal regulated kinase is activated, resulting in skin whitening effects due to the decomposition of microphthalmia-associated transcription factor (MITF). However, an effective method for inhibiting protein phosphatase 2A has not been identified.

[0027] In relation to this, the present inventors have confirmed that when a composition containing a specific compound as an active ingredient (more specifically, a composition containing the specific compound as an active ingredient in a specific concentration range) is treated with normal human melanocytes, it has a melanin synthesis inhibitory effect, and thus completed the present invention.

[0028] Meanwhile, as in conventional methods, substances that directly inhibit melanin-producing enzymes (TRP1, TRP2, tyrosinase) must be used in high concentrations, which can cause side effects such as skin irritation, and kojic acid is prohibited for use due to the possibility of inducing skin cancer. However, the composition according to one embodiment does not directly inhibit melanin-producing enzymes (TRP1, TRP2, tyrosinase), so it does not irritate the skin and has almost no side effects, and can achieve a much safer and more powerful whitening effect by inhibiting melanin production.

[0029] Unless otherwise defined herein, "combination" means blending or copolymerization, "copolymerization" means block copolymerization or random copolymerization, and "copolymer" means block copolymerization or random copolymerization.

[0030] Hereinafter, a skin whitening composition according to one embodiment will be described.

[0031] The composition for skin whitening according to one embodiment includes a compound represented by the following Formula 1 as an active ingredient.

[0032] [ka]

[0033] In the above Chemical Formula 1, X is CRR' (R and R' are each independently a hydrogen atom or a substituted or unsubstituted C1-C10 alkyl group), an oxygen atom, or a sulfur atom; L 1 is a substituted or unsubstituted C1 to C20 alkylene group, R 1 is a hydroxy group, a substituted or unsubstituted C1 to C20 alkyl group, or a substituted or unsubstituted C1 to C20 alkoxy group.

[0034] For example, the compound represented by the formula 1 may be a substance derived from lactobacillus fermentation. Recently, as people's interest in health has increased, the need for skin immune cosmetics has been growing, and efforts have been made to develop microbiome materials that enhance the skin's defense function and have various skin benefits, but there are few materials that can be expected to have a skin whitening effect in this regard. However, the inventors of the present invention have started from the idea that a source of materials that can achieve skin whitening effects can be obtained from microbiome materials, and after several years of research and experimentation on a wide variety of microbiome materials, they have confirmed that the compound represented by the formula 1 derived from lactobacillus fermentation can achieve a skin whitening effect, thereby completing the present invention.

[0035] For example, in Formula 1, X is an oxygen atom, and R 1 can be a hydroxy group.

[0036] For example, the formula 1 may be represented by the following formula 1-1, but is not limited thereto.

[0037] [ka]

[0038] For example, the compound represented by Chemical Formula 1 may be contained in a concentration of 0.01 μM to 10 mM based on the total amount of the composition. When the compound represented by Chemical Formula 1 is contained in the above concentration range, it can effectively inhibit melanin production in melanocytes of normal human skin without cytotoxicity, thereby further doubling the whitening efficacy.

[0039] Specifically, the compound represented by Chemical Formula 1 in the composition may be contained at a concentration of 0.01 μM to 10 mM, for example, at a concentration of 0.1 μM to 10 mM, for example, at a concentration of 1 μM to 10 mM, for example, at a concentration of 0.01 mM to 10 mM, for example, at a concentration of 0.1 mM to 10 mM, relative to the total amount of the composition. For example, the compound represented by Chemical Formula 1 in the composition may be contained at a concentration of 0.01 μM or more, for example, at a concentration of 0.1 μM or more, for example, at a concentration of 1 μM or more, for example, at a concentration of 100 μM or more, relative to the total amount of the composition, and may be contained at a concentration of 10 mM or less, for example, at a concentration of 1 mM or less. When the compound represented by Chemical Formula 1 is used at a concentration of less than 0.01 μM, the degree of suppressing the new production of melanin in melanocytes is negligible, and the compound cannot have an effect of improving the skin whitening function. In addition, when the compound represented by Chemical Formula 1 is used at a concentration exceeding 10 mM, it is not preferable because it may cause cytotoxicity and harm to the human body. Furthermore, when the compound represented by formula 1 is a compound derived from lactobacillus ferment, the compound represented by formula 1 (derived from lactobacillus ferment) in the composition may be contained at a concentration of 10 μM to 10 mM, for example, 100 μM to 5 mM. Compared to the case where the compound represented by formula 1 is separately synthesized and used, the case where the compound represented by formula 1 is used in the form of lactobacillus ferment corresponds to the case where a natural product is used, so the concentration range where the compound inhibits the de novo production of melanin in melanocytes without causing cytotoxicity may differ. This is thought to be because, unlike chemically synthesized products, the desired function expression may be restricted or changed due to interactions with many components contained in the composition.

[0040] Hyperpigmentation of the skin can be caused by many factors, including hormonal abnormalities in the body, genetic diseases, and ultraviolet light exposure following an inflammatory reaction of the skin. The main causes include abnormalities in the synthesis and distribution of melanin pigment.

[0041] The main function of melanin is to remove oxygen radicals and protect the skin from damage caused by them. Therefore, having a lot of melanin means that the skin has an effective response system to protect it from physical and chemical toxic substances. This melanin is produced in melanocytes by the action of enzymes and spontaneous oxidation after tyrosine is converted to dapaquinone by melanin-producing enzymes (TRP1, TRP2, tyrosinase).

[0042] The currently known methods for inhibiting melanin production are broadly as follows.

[0043] First, there is a method of blocking ultraviolet rays to eliminate the main cause of melanin production, and good results can be expected from this method by making the cosmetic composition contain a light scattering agent or a light blocking agent.

[0044] Next, melanin production can be suppressed by inhibiting the synthesis of core carbohydrates, such as glucosamine, which are necessary for the activity of melanin-producing enzymes (TRP1, TRP2, tyrosinase).

[0045] In addition, kojic acid or arbutin can block the function of melanin-producing enzyme 1 (TRP1), an enzyme involved in melanin production.

[0046] In addition, it is also possible to use a substance such as hydroquinone that is specifically toxic to melanocytes that produce melanin to inhibit melanocyte division.

[0047] In addition, a method of decolorizing the skin by reducing the melanin produced has also been introduced.

[0048] Currently, most research into finding whitening compositions focuses on substances that directly inhibit melanin-producing enzymes (TRP1, TRP2, tyrosinase) involved in melanin synthesis. However, in addition to directly inhibiting melanin-producing enzymes (TRP1, TRP2, tyrosinase), substances have been proposed that are expected to have whitening effects by regulating the signal transduction involved in the expression of melanin-producing enzymes (TRP1, TRP2, tyrosinase). (Briganti S, Camera E, Picardo M. Pigment Cell Res, 2003, 16(2):101-10)

[0049] Specifically, the composition according to one embodiment has an excellent effect of inhibiting new melanin production in melanocytes, and therefore has an excellent skin whitening effect when used on skin in which hyperpigmentation has been induced by skin pigmentation inducers.

[0050] In one embodiment, a composition for skin whitening is provided that contains the compound represented by Chemical Formula 1 as an active ingredient, and may contain a pharma- ceutical effective amount of the compound represented by Chemical Formula 1 alone or in combination with one or more pharma- ceutical acceptable carriers, excipients, or diluents.

[0051] The term "pharmacologically effective amount" refers to an amount of the physiologically active ingredient sufficient to exhibit the intended physiological or pharmacological activity when administered to an animal or human. However, the pharmacologic effective amount may vary depending on the severity of symptoms, the patient's age, weight, health condition, sex, administration route, and treatment period.

[0052] In addition, the term "pharmaceutical acceptable" means that it is physiologically acceptable and does not cause allergic reactions such as gastrointestinal disorders and dizziness or similar reactions when administered to humans. Examples of the carrier, excipient, and diluent include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, gum acacia, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil. In addition, fillers, anti-agglomerating agents, lubricants, wetting agents, flavorings, emulsifiers, and preservatives may be further included.

[0053] For example, the composition may be a cosmetic composition.

[0054] In the present specification, the term "cosmetic material" refers to any material that has not only a cosmetic function but also a medical function in addition to the cosmetic function.

[0055] The formulation of the cosmetic composition is not particularly limited and can be appropriately selected depending on the purpose.

[0056] For example, the cosmetic composition may be formulated as, but is not limited to, a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleanser, oil, powder foundation, emulsion foundation, wax foundation, spray, etc. More specifically, the cosmetic composition may be formulated as a cleanser, tonic, hair styling agent, nourishing lotion, essence, serum, treatment, conditioner, shampoo, lotion, hair care agent or hair dye, or as an oil-in-water (O / W) or water-in-oil (W / O) type basic cosmetic. For example, the composition may have one dosage form selected from the group consisting of skin lotion, skin toner, astringent, lotion, milky lotion, moisturizing lotion, massage cream, moisturizing cream, moisturizing cream, hand cream, ointment, foundation, essence, moisturizing essence, pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, body cleanser, lotion, gel, cream, patch and spray. In addition, the composition may have each dosage form in which other ingredients besides the essential ingredients can be appropriately selected and mixed by those skilled in the art according to the type of external preparation or purpose of use. For example, it may further include an ultraviolet blocking agent, a hair conditioning agent, a fragrance, etc.

[0057] Said cosmetic composition may contain a cosmetically acceptable medium or base. It may be in any formulation suitable for topical application, for example in the form of a solution, gel, solid or pasty anhydrous product, emulsion obtained by dispersing an oily phase in an aqueous phase, suspension, microemulsion, microcapsule, microgranule or vesicular dispersion of ionic (liposome) and / or non-ionic type, or in the form of a cream, skin, lotion, powder, ointment, spray or concealer stick. These compositions may be prepared by the usual methods known in the art.

[0058] When the dosage form of the present invention is a solution or emulsion, a solvent, solubilizer or emulsifier is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

[0059] When the dosage form of the present invention is a suspension, the carrier component can be a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar or tragacanth, etc.

[0060] When the dosage form of the present invention is a paste, cream or gel, the carrier component may be an animal oil, a vegetable oil, wax, paraffin, starch, tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide.

[0061] When the dosage form of the present invention is a paste, cream or gel, the carrier component may be an animal oil, a vegetable oil, wax, paraffin, starch, tragacanth, a cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide.

[0062] When the dosage form of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder can be used as a carrier component, and especially when it is a spray, a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether can be additionally contained.

[0063] In one embodiment of the present invention, the cosmetic composition may additionally contain a thickener. The thickener contained in the cosmetic composition of the present invention may be methyl cellulose, carboxymethyl cellulose, carboxymethyl hydroxyguanine, hydroxymethyl cellulose, hydroxyethyl cellulose, carboxyvinyl polymer, polyquaternium, cetearyl alcohol, stearic acid, carrageenan, etc., preferably one or more of carboxymethyl cellulose, carboxyvinyl polymer, and polyquaternium, and most preferably carboxyvinyl polymer.

[0064] In one embodiment of the present invention, the cosmetic composition may contain various suitable bases and additives as necessary, and the types and amounts of these components may be appropriately selected by the inventor. If necessary, acceptable additives may be contained, for example, preservatives, dyes, additives, and other components commonly used in the art may be additionally contained.

[0065] Specific examples of preservatives include phenoxyethanol and 1,2-hexanediol, and examples of fragrances include artificial fragrances.

[0066] In one embodiment of the present invention, the cosmetic composition may contain a composition selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, polymer peptides, polymer polysaccharides, sphingolipids, and seaweed extracts. Other ingredients that may be added include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, bactericides, antioxidants, plant extracts, pH adjusters, alcohol, colorants, fragrances, blood circulation promoters, cooling agents, antiperspirants, purified water, etc.

[0067] Furthermore, other ingredients that may be added are not limited to those mentioned above, and any of the ingredients described above can be added within a range that does not impair the objects and effects of the present invention.

[0068] Furthermore, the cosmetic composition according to an embodiment can be used not only as a pharmaceutical composition as described above, but also as a functional health food, for example, it can be easily used as a main ingredient, a secondary ingredient, a food additive, a functional food, or a beverage.

[0069] The term "food" refers to a natural or processed product that contains one or more nutrients, and preferably refers to a product that has been processed to a certain extent to be in a state that can be eaten directly, and in its usual sense includes all foods, food additives, functional foods, and beverages.

[0070] Examples of foods to which the food composition can be added include various foods, beverages, gums, teas, vitamin complexes, functional foods, etc. In addition, special nutritional foods (e.g., infant formula, baby food, etc.), processed meat products, fish products, tofu, jelly, noodles (e.g., ramen, buckwheat, etc.), bread, dietary supplements, seasonings (e.g., soy sauce, miso, gochujang, mixed sauce, etc.), sauces, confectionery (e.g., snacks), candies, chocolates, gums, ice cream, dairy products (e.g., fermented milk, cheese, etc.), other processed foods, kimchi, pickled foods (various kimchi, jangachi, etc.), beverages (e.g., fruit beverages, vegetable beverages, soy milk, fermented beverages, etc.), and natural seasonings (e.g., ramen soup, etc.) are included, but are not limited thereto. The foods, beverages, or food additives can be prepared by a conventional manufacturing method.

[0071] In addition, the term "functional food" or "health functional food" refers to a food group that has been given added value by using physical, biochemical, or biotechnological techniques to act and express the function of the food for a specific purpose, or a food that has been designed and processed to fully express in the body the internal regulatory functions related to the regulation of the biological defense rhythm, prevention and recovery from diseases, etc., possessed by the food composition, and specifically may be a health functional food. The functional food may include food-scientifically acceptable food supplement additives, and may further include suitable carriers, excipients, and diluents that are commonly used in the manufacture of functional foods.

[0072] The type of dietary supplement may be, but is not limited to, in the form of powder, granules, tablets, capsules or beverages.

[0073] Another embodiment provides a method for whitening the skin of an individual, comprising administering to the individual the composition, which is as described above.

[0074] The administration may be by any method known in the art. The administration may be by any means, such as by intravenous, intramuscular, oral, transdermal, mucosal, intranasal, intratracheal or subcutaneous administration, directly to the individual. The administration may be systemic or local. The administration may be localized to the site of skin aging. The administration may be, for example, by application. The application refers to any method of contacting the composition with the skin of an individual in a suitable manner, thereby allowing the composition to be absorbed into the skin.

[0075] The individual may be a mammal, such as a human, cow, horse, pig, dog, sheep, goat, or cat. The individual may be an individual in need of improving, slowing, or inhibiting skin hyperpigmentation. The individual may be an individual in need of inhibiting cell death, inhibiting protein expression of melanin producing enzyme (tyrosinase), or increasing antioxidant activity.

[0076] The administration may be such that the composition according to one embodiment is administered to one individual per day in an amount of 0.01 mg to 10,000 mg, 0.1 mg to 1,000 mg, for example, 0.1 mg to 500 mg, 0.1 mg to 100 mg, 0.1 mg to 50 mg, 0.1 mg to 25 mg, 1 mg to 1,000 mg, 1 mg to 500 mg, 1 mg to 100 mg, 1 mg to 50 mg, 1 mg to 25 mg, 5 mg to 1,000 mg, 5 mg to 500 mg, 5 mg to 100 mg, 5 mg to 50 mg, 5 mg to 25 mg, 10 mg to 1,000 mg, 10 mg to 500 mg, 10 mg to 100 mg, 10 mg to 50 mg, or 10 mg to 25 mg. Alternatively, the administration may be such that the composition according to one embodiment is administered to act at a working concentration of 0.01 to 25 μM, 0.05 to 5 μM, 0.075 to 3.75 μM, 0.1 to 2.5 μM, 0.15 to 2 μM, 0.25 to 1.5 μM, 0.4 to 1.25 μM, 0.5 to 1.2 μM, or 0.75 to 1.15 μM.

[0077] The advantages and features of the present invention, and the methods for achieving them, will become clearer with reference to the following detailed examples. The present invention will be described in detail below with reference to the following examples. However, these examples are for the purpose of specifically explaining the present invention, and the scope of the present invention is not limited to these examples.

[0078] (Example) Test example 1: Melanocytes (normal human epidermal melanocytes) isolated from normal human skin were treated with the compound represented by the following chemical formula 1-1 (Christian Hansen) dissolved in DMSO at 1000X and added to culture medium at final concentrations of 0μM (control group), 0.1μM, 1μM, and 10μM. The cells were grown in a 37℃, 5% CO2 incubator for up to 8 days, changing the culture medium containing the experimental substance every two days. After the experiment was completed, the cells were dissolved in 1N NaOH, photographed, and the absorbance was measured at 475nm to compare the relative melanin amounts, and the results are shown in Figures 1 and 2. (**Statistically significant difference P value vs control group<0.01)

[0079] [ka]

[0080] 1 and 2, it can be seen that when the compound represented by Chemical Formula 1-1 is included, melanin production in normal human melanocytes is effectively suppressed. In particular, it can be seen that the compound represented by Chemical Formula 1-1 inhibits melanin production in a concentration-dependent manner.

[0081] Test example 2: Normal human epidermal melanocytes isolated from normal human skin were treated with the compound represented by formula 1-1 (Christian Hansen) dissolved in DMSO at 1000X in culture medium to a final concentration of 0μM (control group), 0.1μM, 1μM, and 10μM. The cells were grown in a 37℃, 5% CO2 incubator for up to 8 days, changing the culture medium containing the experimental substance every two days. After the experiment, the medium was washed off, and the cells were cultured for an additional hour in fresh medium containing CCK8 (Cell counting kit, Sigma product). After the additional culture, the medium was collected and the absorbance was measured at 450nm and 650nm. The 650nm value was subtracted from the 450nm value, and the relative cell viability was compared with the control group. The results are shown in Figure 3.

[0082] From FIG. 3, it can be seen that when the compound represented by Chemical Formula 1-1 is included, there is no cytotoxicity compared to the control group.

[0083] 1 to 3, it can be seen that the compound represented by Chemical Formula 1-1 (Christian Hansen) has a melanin production inhibitory effect without cytotoxicity when the concentration is 0.1 μM, 1 μM, and 10 μM, respectively. Considering that when the concentration of an active ingredient is generally confirmed in a cell experiment, the concentration of the active ingredient in the composition actually used is generally considered to be about 100 to 1000 times the concentration of the active ingredient used in the cell experiment, it can be inferred that the compound represented by Chemical Formula 1, which is an active ingredient in the skin whitening composition according to one embodiment, is optimally contained at a concentration of 10 μM to 10 mM relative to the total amount of the composition to achieve a melanin production inhibitory effect without cytotoxicity.

[0084] Although the preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concept of the present invention defined in the claims also fall within the scope of the present invention.

Claims

1. A whitening composition comprising a compound represented by the following formula 1 as an active ingredient: 【Chemistry 1】 (In the above Chemical Formula 1, X is CRR' (R and R' are each independently a hydrogen atom or a substituted or unsubstituted C1-C10 alkyl group), an oxygen atom, or a sulfur atom; L １ is a substituted or unsubstituted C1 to C20 alkylene group, R １ is a hydroxy group, a substituted or unsubstituted C1 to C20 alkyl group, or a substituted or unsubstituted C1 to C20 alkoxy group.

2. In the above Chemical Formula 1, X is an oxygen atom, and R １ The whitening composition according to claim 1 , wherein is a hydroxy group.

3. The whitening composition according to claim 1, wherein the chemical formula 1 is represented by the following chemical formula 1-1: 【Chemistry 2】

4. The whitening composition according to claim 1, wherein the compound represented by Chemical Formula 1 is contained in a concentration of 0.01 μM to 10 mM based on the total amount of the composition.

5. The whitening composition according to claim 1, wherein the compound represented by Chemical Formula 1 is derived from lactobacillus ferment.

6. The whitening composition according to claim 1 , wherein the whitening composition is a cosmetic composition.

Images