Composition containing matsutake extract having anti-aging effect and its use

Abstract

The present application provides a composition having an anti-aging effect, comprising matsutake extract, ergothioneine, and bacteriol. The composition has a good repair effect on photoaging damage of the skin induced by UV, and the components are formulated in different proportions, and the medicinal effect is obvious, which is superior to the simple combination of using each component individually or combining two components, and has a remarkable synergistic effect, and can avoid the appearance of damage states such as dryness, erythema, roughness, wrinkles, and epidermal hyperplasia on the skin, and can significantly reduce the levels of oxidative stress and inflammatory factors IL-1β, IL-6, COX-2, TNF-α, avoid causing photo-damage to the skin, effectively improve the oxidation of the skin, repair the skin barrier function, and protect the skin. The composition according to the present application is a natural plant extract, with appropriate raw material costs and preparation costs, easy to prepare, low in toxic and side effects, and easy to obtain.

Description

Technical Field

[0001] The present application belongs to the technical field of cosmetics, and specifically relates to a composition containing matsutake extract having an anti-aging effect and its use.

Background Art

[0002] The skin is the largest organ of the human body, and its most important role is the barrier function, that is, while preventing damage to the skin from external stimuli, it prevents the dissipation of moisture inside the skin. Along with natural aging, when progressive mitochondrial dysfunction occurs during the aging process, it increases the production of ROS (reactive oxygen species), and the latter may further cause deterioration of mitochondrial function and damage to the entire cell. In general circumstances, radicals in skin cells are subject to the corresponding regulatory mechanisms and are maintained within a certain threshold range to maintain a dynamic balance. A certain amount of radicals plays an important role in aspects such as energy transfer, inflammation removal, and necrotic cell removal. However, when the living body is placed in an adverse environment, or the cells are in a hypoxic state for a long time, or in an environment of strong light or high temperature, etc., excessive ROS cannot be immediately removed, and the radicals accumulated in the living body bring various harms to the cells. Excessive activated oxygen radicals are likely to cause peroxidation reactions, destroy the biological membrane structure, directly threaten the internal environment and steady state inside the cell, and at the same time oxidize macromolecular proteins, cause protein cross-linking and aggregation, and ultimately functional proteins may lose their biological activity.

[0003] In addition, due to the fact that excessive DPPH active oxygen radicals are likely to damage mitochondrial DNA, the genetic information of mitochondria cannot be normally expressed, which seriously affects the operation of the energy conversion enzyme system. For the DNA in the cell nucleus, radicals accumulate it, change the gene sequence, and can induce gene mutations. The oxidative stress reaction caused by excessive ROS in cells causes abnormal cell functions, causes varying degrees of damage to the physiological functions of the living body, and further causes human aging and related diseases.

[0004] Among them, in the natural aging process, skin damage caused by ultraviolet radiation (UVB) is most commonly seen. Since UVB can damage the skin up to the dermis layer, collagen is damaged, problems occur in elastic fibers, the skin sags, wrinkles appear, and when photoaging becomes severe, the wrinkles that appear on the skin are difficult to recover with skin care products, expression lines accumulate day by day, the skin shows an aging state, and expression lines are an important factor indicating that the skin is in an aging state.

[0005] Currently, the main components of most anti-photoaging products are those that use a mixture of non-natural components and adjuvants. The non-natural component they use is often retinol. However, for those who use retinol for the first time, there is a possibility of skin peeling, which is one of the most common side effects of retinol. Such adverse effects disappear spontaneously after the skin adapts to the product. Another common adverse reaction is skin dryness and itching. Such problems can be solved by using moisturizers and other moisturizing components, but they may also have a certain impact on the skin. In the prior art, anti-aging products containing natural plant extracts can be obtained, which have the characteristics of being green, safe and having few side effects, and can avoid the impact of non-natural components on the skin. However, since the main function is to supplement vitamins to the skin, its anti-aging effect has limitations. Therefore, it is an urgent task at present to develop, select and use more effective and easily prepared natural plant extracts to effectively delay skin oxidation and resist skin problems such as aging caused by UV irradiation.

Summary of the Invention

Problems to be Solved by the Invention

[0006] This application provides a composition containing matsutake extract having an anti-aging effect and its use. The composition can effectively relieve skin problems such as skin oxidation and anti-aging, repair the skin barrier function, restore the skin to a healthy state, and aims to provide more efficient anti-aging products.

Means for Solving the Problems

[0007] As a first aspect, the present application provides a composition having an anti-aging effect, which contains matsutake extract, ergothioneine, and bacteriol.

[0008] As can be understood from the study of the present application, the composition containing matsutake extract, bacteriol, and ergothioneine has an obviously superior effect than using each component individually or using a simple combination of two components, has a remarkable synergistic effect, can significantly suppress non-enzymatic glycosylation, has a fairly good anti-aging effect, has a fairly good repair effect on photoaging damage of the skin induced by UV, can avoid the appearance of damage states such as dryness, erythema, roughness, wrinkles, and epidermal hyperplasia on the skin, can significantly reduce the levels of oxidative stress and inflammatory factors IL-1β, IL-6, COX-2, TNF-α, reduce skin inflammation, avoid the occurrence of skin photo-damage, effectively improve skin oxidation, repair the skin barrier function, and protect the skin.

[0009] Furthermore, the mass ratio of the matsutake extract, bacteriol, and ergothioneine is (1 to 10):(0.1 to 1):(0.01 to 0.05).

[0010] Preferably, the mass ratio of the matsutake extract, bacteriol, and ergothioneine is (5 to 10):(0.5 to 1):(0.01 to 0.03).

[0011] Preferably, the mass ratio of the matsutake extract, bacteriol, and ergothioneine is (5 to 7.5):(0.5 to 0.75):(0.01 to 0.02).

[0012] More preferably, the mass ratio of the matsutake extract, bacteriol, and ergothioneine is 5:0.5:0.01.

[0013] The composition with anti-aging effect according to the present application is obtained by scientifically combining components of matsutake extract, ergothioneine, and bacitracin, which are natural extracts. Among them, the matsutake extract can remove mitochondrial terminal DPPH radicals, the ergothioneine has effects such as radical removal, detoxification, and maintenance of DNA biosynthesis, and the bacitracin has an antioxidant effect. The composition according to the present application achieves the anti-aging effect by suppressing non-enzymatic glycosylation reaction, removing radicals, strengthening the skin barrier, etc. Through multiple routes, each component acts synergistically and together repairs the skin barrier function.

[0014] The matsutake extract is prepared from matsutake. Matsutake (TMP) is a fungus that can be used both as medicine and food, and contains extremely rich components such as saccharides, amino acids and polypeptides, volatile substances, vitamins and minerals, and lipid substances. Matsutake has pharmacological effects such as anti-radiation, immune regulation, whitening & skin care, anti-photoaging, anti-diabetes, anti-hypertension, gastrointestinal regulation, antioxidant, anti-cancer, and anti-tumor, and has high edible and medicinal values. In recent years, there have been many studies on the use of matsutake or matsutake extract in the research and development of cosmetics, and it has been proven that the active ingredients contained in matsutake extract have a great impact on aspects such as skin anti-aging, antioxidant, whitening, and anti-wrinkle, and the market prospect is broad. Therefore, the use of matsutake extract in cosmetics is in line with the development trend of modern cosmetics that are safe, natural, and environmentally friendly.

[0015] The preparation method of the matsutake extract used in the present application is to use dried matsutake, add a 75% - 85% ethanol solution at a raw material solution ratio of 1:(5 - 15), heat and extract in a water bath at 70 - 80°C for 1 - 3 h, extract 2 - 3 times, then filter, concentrate, and dilute to obtain the matsutake extract.

[0016] More preferably, the preparation method of the matsutake extract is to use dried matsutake, add a 75% ethanol solution at a raw material solution ratio of 1:10, heat and extract in a water bath at 80°C for 2 h, extract 2 times, then filter, concentrate, and dilute to obtain it.

[0017] Ergothioneine (mercaptohistidine (ergothioneine: EGT)) is a safe and non-toxic natural antioxidant. Ergothioneine is mainly derived from plants such as mushrooms, broccoli, and Brussels sprouts, and has multiple physiological functions such as radical removal, detoxification, maintenance of DNA biosynthesis, normal cell growth, and cellular immunity. It can play a role in protecting cells in the human body and is an important active substance in vivo. Natural antioxidants have become a research hotspot.

[0018] Preferably, the ergothioneine used in the present application may be prepared by a general method in the art or may be used by directly purchasing a product, and the purity of ergothioneine is 98% or more.

[0019] Bactiol is derived from the mature fruits of the leguminous plant, garden bean, and has the effects of sebum control, acne prevention, antibacterial and anti-inflammatory, and can be used to treat skin yellowing and post-inflammatory pigmentation. Its whitening principle is to achieve a certain whitening effect by suppressing the activities of tyrosinemonophenolase and diphenolase. In addition, bactiol has good antioxidant and anti-aging effects, is a more powerful fat-soluble antioxidant than vitamin E, can prevent skin damage caused by ultraviolet rays while suppressing lipid peroxidation induced by radicals, and can delay cell apoptosis.

[0020] Preferably, the bactiol used in the present application may be prepared by a general method in the art or may be used by directly purchasing a product, and the purity of bactiol is 98% or more.

[0021] As a second aspect, the present application provides the use of the composition described in the first aspect in the preparation of anti-aging and anti-photoaging products.

[0022] As a third aspect, the present application further provides an anti-aging and anti-photoaging product containing the composition and adjuvant described in the first aspect.

[0023] Preferably, the mass percentage of the composition contained in the product is 0.01% to 10%.

[0024] More preferably, the mass percentage of the composition contained in the product is 0.03% to 5%.

[0025] Preferably, the adjuvant contains at least one of a humectant, an antioxidant, an emollient, and a preservative.

[0026] Preferably, the product is a skin care product or a cosmetic, and more preferably, the skin care product is a skin care lotion, emulsion, cream, pack, essence, or the like.

Advantages of the Invention

[0027] The present application has the following beneficial effects.

[0028] The present application provides a composition having an anti-aging effect containing matsutake extract, ergothioneine, and bacteriol. As can be seen from the research of the present application, by blending matsutake extract, bacteriol, and ergothioneine, the effect is clearly superior to using each component individually or a simple combination of blending two components, has a remarkable synergistic effect, can significantly suppress non-enzymatic glycosylation, and has a fairly good anti-aging effect. The composition according to the present application has a fairly good repair effect on the photoaging damage of the skin induced by UV, avoids the appearance of damage states such as dryness, erythema, roughness, wrinkles, and epidermal hyperplasia on the skin, significantly reduces the levels of oxidative stress and inflammatory factors IL-1β, IL-6, COX-2, and TNF-α, reduces skin inflammation, avoids the occurrence of skin photo-damage, effectively improves skin oxidation, repairs the skin barrier function, and can protect the skin. It is the same as the synergistic effect.

[0029] The composition according to the present application is a natural plant extract, with appropriate raw material costs and preparation costs, easy to prepare, low in toxic and side effects, easy to obtain, low in price, and provides more efficient anti-aging products for the field of cosmetics.

Brief Description of the Drawings

[0030]

Figure 1

Modes for Carrying Out the Invention

[0031] Hereinafter, the present application will be further described in conjunction with the drawings and specific examples. However, the examples do not limit the present application in any form. Unless otherwise specified, the reagents, methods, and apparatuses used in the present application are common reagents, methods, and apparatuses in the technical field.

Examples

[0032] Unless otherwise specified, the reagents and materials used in the following examples are all commercially available.

[0033] The dried matsutake, bakuchiol, and ergothioneine used in the following examples according to the present application are all commercially available. The test mice are KM mice (age: 6 - 8 weeks) and are provided by Carrot-Biological Company.

[0034] Example 1: Preparation of the composition Matsutake extract: Take 200 g of dried matsutake, add an 80% ethanol solution at a raw material solution ratio of 1:10, heat and extract in a water bath at 85°C for 3 h, extract 2 - 3 times, then filter, concentrate, and dilute to obtain an alcohol extract of matsutake.

[0035] Bacteriol: Purchased from Hangzhou Grascent Co., Ltd, 98% purity.

[0036] Ergothioneine: Purchased from Macklin Biochemical Technology Co., Ltd, 98% purity.

[0037] The composition according to this example was obtained by mixing three components in a mass ratio of 5:0.5:0.01 after obtaining matsutake extract, bacteriol, and ergothioneine respectively according to the above steps. Specifically, 5 g of matsutake extract, 0.5 g of bacteriol, and 0.01 g of ergothioneine were used.

[0038] Example 2: Preparation of composition Matsutake extract: 200 g of dried matsutake was taken, an 80% ethanol solution was added at a raw material solution ratio of 1:10, heated and extracted in a water bath at 85°C for 3 h, extracted 2 - 3 times, then filtered, concentrated, and diluted to obtain an alcohol extract of matsutake.

[0039] Bacteriol: Purchased from Hangzhou Grascent Co., Ltd, 98% purity.

[0040] Ergothioneine: Purchased from Macklin Biochemical Technology Co., Ltd, 98% purity.

[0041] The composition according to this example was obtained by mixing three components in a mass ratio of 7.5:0.75:0.02 after obtaining matsutake extract, bacteriol, and ergothioneine respectively according to the above steps. Specifically, 7.5 g of matsutake extract, 0.75 g of bacteriol, and 0.02 g of ergothioneine were used.

[0042] Example 3: Preparation of composition Matsutake extract: Take 200 g of dried matsutake, add an 80% ethanol solution at a raw material solution ratio of 1:10, heat and extract in a water bath at 85 °C for 3 h, extract 2 - 3 times, then filter, concentrate, and dilute to obtain an alcohol extract of matsutake.

[0043] Bactiol: Purchased from Hangzhou Grascent Co., Ltd, with a purity of 98%.

[0044] Ergothioneine: Purchased from Macklin Biochemical Technology Co., Ltd, with a purity of 98%.

[0045] The composition according to this example is obtained by mixing three components in a mass ratio of 10:1:0.03 after obtaining matsutake extract, bactiol, and ergothioneine respectively according to the above steps. Specifically, 10 g of matsutake extract, 1.0 g of bactiol, and 0.03 g of ergothioneine were used.

[0046] Example 4: Preparation of compositions with different components In this application, multiple types of compositions with different components and contents were studied and prepared for application in subsequent detection research. The specific components and contents are as shown in Table 1 below. The preparation method of matsutake extract is the same as that in Example 1. Bactiol was purchased from Hangzhou Grascent Co., Ltd with a purity of 98%, and ergothioneine was purchased from Macklin Biochemical Technology Co., Ltd with a purity of 98%.

[0047]

Table 1

[0048] Test Example 1: Inhibitory test on the non - enzymatic glycosylation reaction of different compositions To a 100 mL, pH = 7.4, 0.2 mol / L phosphate buffer solution, 19.82 g of glucose, 0.4024 g of bovine serum albumin and 0.39 g of sodium azide were added. After uniform mixing, a reaction solution was formed.

[0049] The compositions prepared in Examples 1 to 3 and Cases 1 to 11 were each prepared as test solutions with different concentrations using distilled water, and the prepared concentrations were 50 μg / mL, 100 μg / mL, 200 μg / mL, 400 μg / mL, 600 μg / mL, 800 μg / mL, and 1000 μg / mL.

[0050] Fourteen test tubes were taken, and 4 mL of the reaction solution and 1 mL of the test solution with different concentrations were sequentially added to each test tube, and the total volume of the solution in all test tubes was made 5 mL each. After measuring the initial fluorescence values of all samples with a fluorescence spectrophotometer, all test tubes were placed in a 37 °C water bath and stored for 7 days in the dark. The fluorescence values of the samples in each test tube were measured. Furthermore, after continuously storing all test tubes in a 37 °C water bath in the dark for 7 days, the fluorescence values were measured. The measurement conditions were an excitation wavelength of 370 nm, a slit of 5 nm, an emission wavelength range of 380 - 500 nm, a slit of 5 nm, and a light source voltage of 700 V.

[0051] As shown in Table 2 below, the inhibitory results of different compositions on the non-enzymatic glycosylation reaction were measured, and it was found that the compositions of Examples 1 to 3 and Cases 1 to 11 all had the activity to inhibit the non-enzymatic glycosylation reaction, but there were differences in the inhibitory ability against the non-enzymatic glycosylation reaction. The compositions of Examples 1 to 3 had good inhibition rates against the non-enzymatic glycosylation reaction at different concentrations, with the inhibition rates ranging from 63.46% to 78.94%. They were significantly superior to the compositions of other cases at the same concentration, and the effect of the composition of Example 1 was optimal according to the blending ratio, and the inhibition rate could reach 78.94%. In Cases 1 to 3 containing only one component, the inhibitory effect on the non-enzymatic glycosylation reaction was relatively poor. In Cases 4 to 9, the inhibitory effect of the compositions using two components on the non-enzymatic glycosylation reaction was slightly improved compared to Cases 1 to 3, but the effect was still significantly lower than that of the compositions of Examples 1 to 3. Even if the effects of two components were simply combined with the effects of a single component, the effect was not as good as that of the compositions of Examples 1 to 3. In Cases 10 to 11, the compositions obtained by adjusting the addition ratio of matsutake extract and blending had a gradually improved inhibitory effect on the non-enzymatic glycosylation reaction compared to Cases 1 to 9, and were better than the effects of a single component and two components, but were not as excellent as the effects of Examples 1 to 3 of the present application.

[0052]

Table 2

[0053] In short, it was found that the composition containing matsutake extract, bacitracin, and ergothioneine had a good inhibitory effect on non-enzymatic glycosylation reaction, had a quite good anti-aging effect, and the medicinal effect was clearly superior to the simple combination of using each component individually or blending two components, and had a significant synergistic effect.

[0054] Test Example 2: Protective effect of the composition against aging induced by UVB The compositions prepared in Example 1, Example 3, Case 1, and Case 6 above were each selected, and their protective effects against UVB-induced skin damage in Kunming mice were evaluated. The effects of the compositions on mouse skin were observed by skin histology and pathological sections. Normal Kunming mice (6-8 weeks old) were selected, their back regions were depilated, and the regions were irradiated with ultraviolet light (UVA irradiation intensity cumulatively reached 148.47 J / cm 2 , and UVB radiation intensity cumulatively reached 48.67 J / cm 2 .), and drug application treatment was performed. Then, the test mice were divided into a blank control group (C), a model control group (MC), a low-dose composition group (Group D), a high-dose composition group (Group F), a composition group without matsutake extract (Group Y), and a group containing only a low dose of matsutake extract (Group G).

[0055] As test drugs, different compositions were each dissolved in a 30% aqueous ethanol solution, and the concentrations of the drugs in each group were controlled to be the same. Among them, 2 mL of a 30% aqueous ethanol solution was directly applied to the blank control group (C) and the model control group (MC). The low-dose composition group (D) was applied with 2 mL of a drug solution formulated using the composition of Example 1 (5% matsutake extract + 0.5% bacteriol + 0.01% ergothioneine). The high-dose composition group (F) was applied with 2 mL of a drug solution formulated using the composition of Example 3 (10% matsutake extract + 1% bacteriol + 0.03% ergothioneine). The drug treatment group (Y) was applied with 2 mL of a solution using the composition of Case 6 (0.5% bacteriol + 0.01% ergothioneine). The drug treatment group (G) was applied with 2 mL of a solution using the composition of Case 1 (5% matsutake extract). Each time, the drug was applied 15 minutes after UVB irradiation. Every week, the mice were treated to observe the condition of the mice, and the evaluation criteria for mouse photoaging were optimized by combining the conditions of the prior art and the test samples. As shown in Table 2, the skin changes of the mice were further evaluated.

[0056]

Table 2

[0057] As shown in Figure 1, in Figure 1(a), by observing the conditions of wrinkles, dryness, and erythema of mouse skin, the following was found. Mice treated locally with the composition drug had these changes significantly improved by UVB radiation. Compared with the healthy back skin in the normal control group (C), the model group (MC) irradiated with ultraviolet light promoted skin dryness, erythema, roughness, and wrinkles, and ultimately obvious flesh-colored skin damage appeared. After applying the composition (D) of Example 1 topically, there was a tendency to suppress large-area erythema and leather-like appearance caused by UVB. Its effect was clearly superior to those of the F and Y groups. Although the G group treated only with the shiitake extract drug had a better repair effect on photoaging damage than the Y group without the shiitake extract, the effect was still not as significant as that of the composition in the D group. As can be clearly seen, by using the composition of Example 1, skin damage caused by UVB can be effectively improved. The results of evaluating the back of the mouse by combining Table 2 are shown in Figure 1(b), indicating that the epidermis of the back of the mouse became hypertrophic.

[0058] Furthermore, quantitative analysis of the average thickness of the epidermal tissue was performed by HE staining to verify the effect of UV on epidermal hyperplasia. The results are shown in Figure 1(c). The average epidermal density in the D group was significantly improved compared with the model group. The repair effect in the F group was slightly inferior to that in the D group, and the repair effect in the G group was inferior to that in the D group next. It can be easily seen that the repair effect in the Y group without adding the shiitake extract was not significant. The results of evaluating the skin thickness of the mouse by combining Table 2 are shown in Figure 1(d), indicating that the D group and the F group significantly reduced the skin thickness of the epidermis of the mouse.

[0059] As can be seen from the above results, the treatment groups of the compositions of Example 1 and Example 3 can significantly reduce phenomena such as erythema, wrinkles, edema, and epidermal hyperplasia of the skin on the back of mice induced by UVB, and have a protective effect on skin damage in mice induced by UVB radiation.

[0060] Test Example 3: Protective Effect of the Composition against Oxidative Stress and Inflammation Oxidative stress and inflammation are factors that cause photoaging of the skin. Further, it was studied by enzyme-linked immunosorbent assay (ELISA). Skin tissues of mice in the blank group, model group, and the composition treatment group (D) of Example 1 were collected. The vitamin E group was used as a positive control (the control treatment was performed according to the treatment method and conditions of Example 6). Skin tissues of the treated mice were collected. Skin tissues of each group were homogenized to obtain supernatants, and the levels of IL-1β, IL-6, COX-2, and TNF-α in the homogenates of the skin tissues were measured. For the specific operation, strictly refer to the operation manual of the kit.

[0061] As shown in Table 4, due to the induction of UVB, compared with the blank group, the inflammatory factors IL-6 and TNF-α in the skin of the model group were significantly increased (P<0.01), and it was also shown that IL-1β and COX-2 also tended to increase. In addition, compared with the blank group, the composition of Example 1 significantly decreased IL-1β in the skin (P<0.05), significantly decreased IL-6 in the skin to 63.55±42.81 pg / mL (P<0.05), significantly decreased COX-2 to 3.53±2.22 ng / mL (P<0.05), and significantly decreased TNFα to 5.95±3.39 pg / mL (P<0.01). Compared with the model group, vitamin E could significantly decrease the levels of COX-2 and TNF-α (P<0.05). Compared with the vitamin E group, IL-1β, IL-6, TNF-α, and COX-2 in the composition group of Example 1 all tended to decrease, and its effect was superior to that of the vitamin E group.

[0062]

Table 4

[0063] As can be seen from the above results, the composition in Example 1 according to the present application can significantly reduce the levels of oxidative stress and inflammatory factors IL-1β, IL-6, COX-2, and TNF-α, avoid causing skin photo-damage, and has a protective effect against skin damage in mice induced by UVB radiation.

[0064] Example 5 This example was set up in the same way as Example 1. The only difference is that the raw material solution ratio in the extraction method of the matsutake extract is different, and the matsutake alcohol extract was obtained by extraction at a raw material solution ratio of 1:5.

[0065] Example 6 This example was set up in the same way as Example 1. The only difference is that the raw material solution ratio in the extraction method of the matsutake extract is different, and the matsutake alcohol extract was obtained by extraction at a raw material solution ratio of 1:15.

[0066] Example 7 This example was set up in the same way as Example 1. The only difference is that the extraction temperature in the extraction method of the matsutake extract is different, and the matsutake alcohol extract was obtained by extraction in a water bath at 70°C.

[0067] Example 8 This example was set up in the same way as Example 1. The only difference is that the extraction temperature in the extraction method of the matsutake extract is different, and the matsutake alcohol extract was obtained by extraction in a water bath at 80°C.

[0068] Example 9 This example was set up in the same way as Example 1. The only difference is that the ethanol solution used in the extraction method of the matsutake extract is different, and the matsutake alcohol extract was obtained by extraction with a 75% ethanol solution.

[0069] Example 10 This example is provided in the same way as Example 1. The only difference is that the ethanol solution used in the extraction method of matsutake extract is different, and an 85% ethanol solution was used for extraction to obtain an alcohol extract of matsutake.

[0070] Using the compositions and their products in Examples 5 to 10 above, the non-enzymatic glycosylation reaction activity was measured. The measurement method is the same as in Test Example 1, and the anti-aging effects of Examples 5 to 10 were evaluated. As a result, in Examples 5 to 10, the inhibitory effects of the matsutake extract prepared by adjusting the extraction conditions of the matsutake extract on the non-enzymatic glycosylation reaction were all lower than those in Example 1, but the inhibition rates on the non-enzymatic glycosylation reaction were 43% to 84%, having a good anti-aging effect, and the effect was still superior to that of the single-component or two-component compositions described in Cases 1 to 8 of Example 4.

[0071] Furthermore, the composition according to the present application is used in skin care products or cosmetics such as creams, emulsions, essences, etc. The skin care products or cosmetics also have the effects of wrinkle removal, antioxidant, and repair of photoaging damage, and the adaptability between the composition according to the present application and the components of other raw materials is good, and the anti-aging effect and function of the composition can be exerted to protect the skin.

[0072] Example 11 The present application provides a cream which is an anti-aging skin care product including Example 1. By mass%, it contains 0.3 wt% of the composition of Example 1, 0.3 wt% of ceramide, 1.0 wt% of lecithin-based, 0.5 wt% of plant sterol, 7.5 wt% of polyol, 0.3 wt% of chelating agent, 3 wt% of thickener, 3.5 wt% of emulsifier, 20.0 wt% of emollient, 5 wt% of aliphatic alcohol, 3 wt% of skin conditioner, 0.01 wt% of antioxidant, 0.01 wt% of preservative, 1.0 wt% of fragrance, and deionized water to make it 100.0 wt%. Among them, the lecithin-based is one or more selected from lecithin, hydrogenated lecithin, and hydroxylated lecithin.

[0073] Example 12 This application provides an emulsion which is an anti-aging skin care product containing Example 1. By weight percentage, it contains 1.0 wt% of the composition of Example 1, 30.0 wt% of glycerin, 4.0 wt% of sorbitol stearate, 3.0 wt% of sucrose coconut fatty acid ester, 5.0 wt% of dioctyl carbonate, 2.0 wt% of paraffin, 3.0 wt% of citric acid, 0.1 wt% of vitamin C, 1.0 wt% of sodium stearate, and deionized water to make up 100.0 wt%.

[0074] Example 13 This application provides an essence which is an anti-aging skin care product containing Example 1. By weight percentage, it contains 5.0 wt% of the composition of Example 1, 40.0 wt% of butanediol, and deionized water to make up 100.0 wt%.

[0075] The above examples are preferred embodiments of this application, but the embodiments of this application are not limited to the above examples. Any changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of this application are all equivalent substitution forms and are all included within the protection scope of this application.

Claims

1. A composition having an anti-aging effect, A composition comprising a matsutake extract, bakuchiol and ergothioneine.

2. The composition of claim 1, wherein the mass ratio of the matsutake extract, bakuchiol and ergothioneine is (1-10):(0.1-1):(0.01-0.05).

3. The composition of claim 2, wherein the mass ratio of the matsutake extract, bakuchiol and ergothioneine is (5-10):(0.5-1):(0.01-0.03).

4. The composition of claim 3, wherein the mass ratio of the matsutake extract, bakuchiol and ergothioneine is (5-7.5):(0.5-0.75):(0.01-0.02).

5. The composition according to any one of claims 1 to 4, wherein the preparation method of the matsutake extract is to use dried matsutake, add 75% to 85% ethanol solution in a raw material solution ratio of 1:(5 to 15), heat extract in a water bath at 70 to 80°C for 1 to 3 hours, extract 2 to 3 times, filter, concentrate and dilute to obtain the matsutake extract.

6. Use of the composition according to any one of claims 1 to 4 in the preparation of an anti-aging and anti-photoaging product.

7. The use according to claim 6, wherein the product is a skin care product or a cosmetic product.

8. An anti-aging and anti-photoaging product comprising the composition according to any one of claims 1 to 4 and an auxiliary agent.

9. The product according to claim 8, wherein the weight percentage of the composition according to any one of claims 1 to 4 contained in said product is 0.01% to 10%.

10. 9. The product of claim 8, wherein the adjuvant comprises at least one of a moisturizer, an antioxidant, an emollient, and a preservative.

11. The product according to any one of claims 8 to 10, wherein the product is a skin care product or a cosmetic product.

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