Ionically curable hydrogel composition containing extracellular polysaccharides

Abstract

A hydrogel composition is provided, comprising a first gelling agent and a second gelling agent, wherein the first gelling agent may include alginate, carrageenan, and gellan gum, and the second gelling agent may be succinoglycan.

Description

[Technical Field]

[0001] The present invention relates to an ionically curable hydrogel composition containing an exopolysaccharide, and more specifically, to a hydrogel composition that contains succinoglycan as a second gelling agent in addition to a first gelling agent, thereby preventing syneresis and providing an excellent feel when used. [Background technology]

[0002] Hydrogels are substances in which hydrophilic polymer chains are cross-linked to form a three-dimensional network structure. They contain a large amount of water and have unique properties, such as being thermodynamically stable in aqueous solutions. As a result, they are actively used in a variety of fields, including medicine, cosmetics, and industry.

[0003] These hydrogels contain gelling agents such as carrageenan, cellulose, and xanthan gum to form a hard film. However, conventional hydrogels prepared using only these gelling agents have problems such as syneresis, where water is separated from the gel, or a somewhat heavy feel when used. Summary of the Invention [Problem to be solved by the invention]

[0004] The present invention has been made to solve the above problems, and an object of the present invention is to provide a hydrogel composition that contains a large amount of water but minimizes the syneresis phenomenon, and has an excellent feel when applied to the skin and when used.

[0005] The technical problems of the present invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those skilled in the art from the following description. [Means for solving the problem]

[0006] An embodiment of the present invention provides a hydrogel composition, comprising a first gelling agent and a second gelling agent, wherein the first gelling agent may include alginate, carrageenan, and gellan gum, and the second gelling agent may be succinoglycan.

[0007] The alginate, carrageenan, gellan gum, and succinoglycan may be contained in a weight ratio of 1:0.5-8:0.5-2:0.5-4.

[0008] The alginate may be contained in an amount of 0.01 to 10% by weight based on the total weight of the composition.

[0009] The carrageenan may be contained in an amount of 0.01 to 10% by weight based on the total weight of the composition.

[0010] The gellan gum may be contained in an amount of 0.01 to 10% by weight based on the total weight of the composition.

[0011] The succinoglycan may be contained in an amount of 0.01 to 10% by weight based on the total weight of the composition.

[0012] In addition, the first gelling agent may include at least one selected from the group consisting of glucomannan, xanthan gum, gelatin, chitosan, collagen, hyaluronic acid, gum arabic (Acacia Senegal Gum), pullulan, pectin, cellulose, sclerotium gum, tara gum (Caesalpinia Spinosa Gum), agar, locust bean gum, and guar gum (Cyamopsis Tetragonoloba (Guar) Gum).

[0013] The composition may further include at least one of a hardener and a moisturizer.

[0014] The curing agent may contain at least one of a divalent metal ion, a trivalent metal ion, and a metal salt.

[0015] The metal ions include calcium (Ca 2+ ), magnesium (Mg 2+ ), barium (Ba 2+ ), manganese (Mn 2+ ), Nickel (Ni 2+ ), copper (Cu 2+ ), zinc (Zn 2+ ), iron (Fe 3+ ) and aluminum (Al 3+ ) may contain one or more selected from the group consisting of

[0016] In addition, the metal salt may include at least one selected from the group consisting of calcium chloride, calcium hydroxide, calcium nitrate, and calcium sulfide.

[0017] The curing agent may be contained in an amount of 0.01 to 5% by weight based on the total weight of the composition.

[0018] In addition, the moisturizer may include at least one selected from the group consisting of glycerin, propylene glycol, dipropylene glycol, butylene glycol, pentylene glycol, and sorbitol.

[0019] The moisturizer may be contained in an amount of 0.01 to 35% by weight based on the total weight of the composition.

[0020] According to an embodiment of the present invention, there is provided a method for preparing a hydrogel composition, the method comprising the steps of: mixing a first gelling agent and a second gelling agent with purified water to obtain a mixture; and dropping the mixture onto a curing agent, wherein the first gelling agent may include alginate, carrageenan, and gellan gum, and the second gelling agent may be succinoglycan. [Effects of the Invention]

[0021] The hydrogel composition according to the present invention has excellent physical properties such as swelling, wettability, and strength, and is characterized by minimizing water evaporation and syneresis even though it contains a large amount of water inside the gel.

[0022] Furthermore, the hydrogel composition according to the present invention is easy to apply to the skin and has the advantages of providing an excellent rolling sensation and residual sensation. BEST MODE FOR CARRYING OUT THE INVENTION

[0023] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by a skilled artisan in the art to which the present invention belongs. In general, the nomenclature used herein is well known and commonly used in the art. Furthermore, in describing embodiments of the present invention, if a detailed description of related known structures or functions is deemed to hinder understanding of the embodiments of the present invention, the detailed description will be omitted. Furthermore, although embodiments of the present invention will be described below, the technical concept of the present invention is not limited thereto and may be modified and implemented in various ways by those skilled in the art.

[0024] The present invention relates to a hydrogel composition, and more particularly to a hydrogel composition that has excellent physical properties such as hydrogel swelling, wettability, or strength, and that provides an excellent feeling when used, such as a rolling sensation or residual sensation.

[0025] The present inventors have used extracellular polysaccharides produced by microorganisms as a gelling agent to optimally adjust the hydrogel properties such as wettability, swelling, and strength, and as a result have developed a hydrogel composition that has excellent rolling and residual sensations.

[0026] The hydrogel compositions of the present invention can contain two or more gelling agents.

[0027] The gelling agent can form a film to contain a large amount of water and adjust the strength of the hydrogel. Specifically, the gelling agent can include two types of gelling agents, a first gelling agent and a second gelling agent. When the second gelling agent is included in addition to the first gelling agent, the strength of the gel can be adjusted more stably and the water content of the gel can be increased, providing excellent moisturizing effect and usability.

[0028] According to an embodiment, the first gelling agent may include at least one of alginate, carrageenan, and gellan gum. For example, the first gelling agent may include all of alginate, carrageenan, and gellan gum. When alginate, carrageenan, and gellan gum are used in combination as the first gelling agent, the hydrogel may efficiently absorb water, and evaporation of the absorbed water may be minimized.

[0029] For example, the alginate may be contained in an amount of 0.01 to 10% by weight, specifically 0.01 to 5% by weight, and more specifically 0.01 to 1.2% by weight, relative to the total weight of the composition.

[0030] Furthermore, for example, carrageenan may be contained in an amount of 0.01 to 10% by weight, specifically 0.01 to 5% by weight, and more specifically 0.01 to 2% by weight, relative to the total weight of the composition.

[0031] As another example, gellan gum may be included in an amount of 0.01 to 10% by weight, specifically 0.01 to 5% by weight, and more specifically 0.01 to 1.2% by weight, relative to the total weight of the composition.

[0032] If the alginate, carrageenan or gellan gum is contained in an amount outside the above range, a gel may not be formed, or even if a gel is formed, the feeling in use may be reduced.

[0033] According to an embodiment, the first gelling agent may further include at least one selected from the group consisting of glucomannan, xanthan gum, gelatin, chitosan, collagen, hyaluronic acid, gum arabic (Acacia Senegal Gum), pullulan, pectin, cellulose, sclerotium gum, tara gum (Caesalpinia Spinosa Gum), agar, locust bean gum, and guar gum (Cyamopsis Tetragonoloba (Guar) Gum), but is not limited thereto.

[0034] According to an embodiment, the second gelling agent may be succinoglycan, an extracellular polysaccharide. Succinoglycan is a polysaccharide produced by fermentation of Sinorhizobium meliloti or Agrobacterium tumefaciens. Together with the first gelling agent, succinoglycan may increase the strength of the hydrogel, preventing the gel from collapsing, allowing the hydrogel to contain more water while minimizing water evaporation from the hydrogel.

[0035] Generally, existing hydrogel compositions contain only a first gelling agent to form a hard film, and such hydrogel compositions have problems such as syneresis, where water separates from the gel, or poor usability due to difficulty in application and rolling on the skin. However, the present invention minimizes syneresis by including succinoglycan as a second gelling agent in addition to the first gelling agent typically used as a hydrogel gelling agent, thereby providing an excellent usability, such as a soft application feeling with a rolling sensation upon application to the skin, and a sustained moisturizing effect due to the composition remaining on the skin.

[0036] According to an embodiment, succinoglycan may be contained in an amount of 0.01 to 10 wt % of the total weight of the composition, specifically 0.01 to 5 wt %, and more specifically 0.01 to 1.2 wt %. If succinoglycan is contained in an amount less than 0.01 wt % of the total weight of the composition, syneresis of the hydrogel may occur, whereas if it is contained in an amount greater than 10 wt % of the total weight of the composition, the gel may become too strong and collapse.

[0037] Specifically, in the present invention, for example, the first gelling agent may be alginate, carrageenan, or gellan gum, and the second gelling agent may be succinoglycan. The inventors have confirmed that by using the above-mentioned components for the first gelling agent and the second gelling agent, a synergistic effect is achieved in terms of the physical properties (e.g., swelling property, wettability, or strength) and the feel when used.

[0038] According to an embodiment, alginate, carrageenan, gellan gum, and succinoglycan may be included in a weight ratio of 1:0.5-8:0.5-2:0.5-4, specifically 1:2-6:1-2:1-4. By adjusting the weight ratio of alginate, carrageenan, gellan gum, and succinoglycan within this range, a hydrogel film is tightly formed and does not lose its shape, syneresis is minimized, and usability such as rolling sensation and residual sensation can be improved.

[0039] As described above, the present invention includes succinoglycan as a second gelling agent in addition to a first gelling agent, which is generally used as a gelling agent for hydrogels. This allows for stable adjustment of physical properties such as strength, swelling, and wettability of hydrogels containing a large amount of water, and minimizes syneresis. Furthermore, the hydrogel according to the present invention is characterized by excellent sensations when used, such as rolling sensation and residual sensation.

[0040] According to an embodiment, the hydrogel composition of the present invention may further comprise at least one of a hardening agent and a moisturizing agent.

[0041] The curing agent can control the curing rate of the gel. Specifically, the curing agent (e.g., metal ions) can crosslink the first and second gelling agents as polysaccharides to form a hydrogel film.

[0042] According to an embodiment, the curing agent may include at least one of divalent or higher valent metal ions, specifically, divalent metal ions, trivalent metal ions, and metal salts. For example, the divalent metal ions may include calcium (Ca 2+ ), magnesium (Mg 2+ ), barium (Ba 2+ ), manganese (Mn 2+ ), Nickel (Ni 2+ ), copper (Cu 2+ ), zinc (Zn 2+ ), and the trivalent metal ion may include one or more selected from the group consisting of iron (Fe 3+ ) and aluminum (Al 3+ The present invention may include, but is not limited to, one or more selected from the group consisting of:

[0043] In addition, the metal salt may include, but is not limited to, one or more selected from the group consisting of calcium chloride, calcium hydroxide, calcium nitrate, and calcium sulfide.

[0044] In some embodiments, the curing agent may be present in an amount of 0.01 to 5 wt % based on the total weight of the composition. If the curing agent is present in an amount less than 0.01 wt % based on the total weight of the composition, the gel may take a long time to harden, while if the curing agent is present in an amount greater than 5 wt % based on the total weight of the composition, the gel may harden too quickly, resulting in poor moldability.

[0045] The moisturizer can provide moisturizing effects such as supplying moisture to the skin and minimizing evaporation of moisture.

[0046] According to an embodiment, the moisturizer may include, but is not limited to, one or more selected from the group consisting of glycerin, propylene glycol, dipropylene glycol, butylene glycol, pentylene glycol, and sorbitol.

[0047] In some embodiments, the humectant may be present in an amount of 0.01 to 35 wt %, more specifically, 0.01 to 30 wt %, based on the total weight of the composition. If the humectant is present in an amount less than 0.01 wt %, the moisturizing effect may be insignificant, and if the humectant is present in an amount greater than 35 wt %, the strength or properties of the hydrogel may not be adequately controlled.

[0048] According to embodiments, the hydrogel composition of the present invention may further contain other ingredients commonly used in the art, such as preservatives, thickeners, pH adjusters, tonicity agents, surfactants, stabilizers, preservatives, UV absorbers, bactericides, blocking agents, antioxidants, organic pigments, inorganic pigments, fragrances, vitamins, etc. The amounts of the above ingredients can be easily selected by those skilled in the art within the range that does not impair the objectives and effects of the present invention.

[0049] Depending on the embodiment, the hydrogel composition of the present invention may be prepared as functional cosmetics such as nourishing creams, essences, and ampoules, or as basic cosmetics such as skin toners and lotions. It may also be prepared as soaps, cleansers, cleansing creams, cleansing waters, or hair cleansing products such as shampoos, rinses, hair conditioners, and hair gels. It may also be prepared as products that are attached to the skin, such as packs and hydrogel slimming patches, or it may be processed into a spherical shape and used in the form of capsules.

[0050] The hydrogel composition of the present invention can be prepared through the following method.

[0051] First, a mixture can be obtained by mixing a first gelling agent and a second gelling agent with purified water. According to an embodiment, the first gelling agent can include at least one of alginate, carrageenan, and gellan gum. For example, the first gelling agent can include all of alginate, carrageenan, and gellan gum. When alginate, carrageenan, and gellan gum are used in combination as the first gelling agent, the hydrogel can efficiently absorb water, and evaporation of the absorbed water can be minimized.

[0052] In some embodiments, the second gelling agent may be succinoglycan. By mixing succinoglycan as a second gelling agent in addition to a first gelling agent, which is generally used as a gelling agent for hydrogels, with purified water, the present invention can produce a composition that minimizes syneresis and provides an excellent feel when applied to the skin, such as a soft rolling sensation upon application and a feeling of the composition remaining on the skin, providing sustained moisturizing effects.

[0053] According to an embodiment, the physical properties of the hydrogel and the usability of the composition can be maximized by appropriately adjusting the mixing ratio of the first gelling agent and the second gelling agent. For example, when the first gelling agent is alginate, carrageenan, and gellan gum and the second gelling agent is succinoglycan, the alginate, carrageenan, gellan gum, and succinoglycan can be mixed in a weight ratio of 1:0.5-8:0.5-2:0.5-4, specifically, 1:2-6:1-2:1-4.

[0054] According to embodiments, in addition to the first and second gelling agents, the purified water may further contain other ingredients commonly used in the art within the scope of the present invention, such as preservatives, thickeners, pH adjusters, tonicity agents, surfactants, stabilizers, preservatives, UV absorbers, disinfectants, blocking agents, antioxidants, organic pigments, inorganic pigments, fragrances, vitamins, etc. The amounts of the ingredients can be easily selected by those skilled in the art within the scope of the present invention.

[0055] The mixture can then be dropped into a hardener, which physically crosslinks the polymer chains of the mixture and hardens it into a gel structure. Specifically, the first gelling agent and the second gelling agent are polysaccharides, and Ca 2+ It can form cross-links with divalent or higher metal ions such as ions to form hydrogel membranes.

[0056] According to an embodiment, the curing agent may include at least one of a divalent metal ion, a trivalent metal ion, and a metal salt. For example, the divalent metal ion may be calcium (Ca 2+ ), magnesium (Mg 2+ ), barium (Ba 2+ ), manganese (Mn 2+ ), Nickel (Ni 2+ ), copper (Cu 2+ ) and zinc (Zn 2+ ), and the trivalent metal ion may include one or more selected from the group consisting of iron (Fe 3+ ) and aluminum (Al 3+The present invention may include, but is not limited to, one or more selected from the group consisting of:

[0057] Furthermore, for example, the metal salt may include one or more selected from the group consisting of calcium chloride, calcium hydroxide, calcium nitrate, and calcium sulfide, but is not limited thereto.

[0058] According to an embodiment, the mixture may be dripped into the aqueous solution containing the curing agent using a syringe pump, but the dripping method is not limited thereto and various other methods may be used, such as dripping using a dropper, pipette, or burette.

[0059] Depending on the embodiment, the method of forming the mixture into a gel structure may be other than the dropping method. For example, the hydrogel composition may be prepared by pouring the mixture into a mold. In this case, the hydrogel composition may be prepared into hydrogel soap, mask packs, patches, etc.

[0060] Alternatively, the hydrogel composition may be prepared in the form of a patch by, for example, applying the mixture to a nonwoven fabric, adhering it to a release film, or subjecting it to a series of processes such as rolling or cutting. In this case, the hydrogel composition may be prepared in the form of a mask pack or the like.

[0061] The hydrogel composition prepared in this manner contains a first gelling agent, which is commonly used as a gelling agent for hydrogels, and also contains succinoglycan as a second gelling agent, which allows the formation of a firm hydrogel membrane and minimizes syneresis. Furthermore, the hydrogel composition has excellent physical properties such as swelling, wettability, and strength, and is characterized by maximizing the sensation of use, including rolling sensation and residual sensation.

[0062] <Experimental Example>

[0063] The following examples are provided to more specifically explain the present invention, but the present invention is not limited thereto.

[0064] Preparation of Examples and Comparative Examples

[0065] (1) Preparation of Examples 1 to 5

[0066] Examples of compositions according to the present invention were prepared using the components set forth in Table 1 below, where the component contents are in weight percent.

[0067] Examples 1 to 5 are hydrogel compositions in which the weight ratio of the first gelling agent to the second gelling agent was adjusted within the range of the examples of the present invention.

[0068] [Table 1]

[0069] Specifically, a preservative, a moisturizer, and a gelling agent were mixed in water and stirred uniformly. Then, the mixture was dropped into an aqueous solution containing a curing agent to cure the mixture, thereby completing the compositions of Examples 1 to 5. 。

[0070] ( 2) Production of Comparative Examples 1 to 11

[0071] Comparative examples of compositions according to the present invention were prepared using the components listed in Table 2 below. The component contents in Table 2 are in weight percent.

[0072] Comparative Examples 1 to 11 are compositions in which at least one of the four components constituting the first gelling agent and the second gelling agent has been removed.

[0073] [Table 2]

[0074] The specific manufacturing method is the same as in Examples 1 to 5.

[0075] (3) Preparation of Comparative Examples 12 to 15

[0076] Comparative examples of compositions according to the present invention were prepared using the components listed in Table 3 below. The component contents in Table 3 are in weight percent.

[0077] Comparative Examples 12 to 15 are compositions in which the ratio of the first gelling agent to the second gelling agent was adjusted outside the range of the examples of the present invention.

[0078] [Table 3]

[0079] The specific manufacturing method is the same as in Examples 1 to 5.

[0080] Experimental Example

[0081] (1) Experimental Example 1: Evaluation of the physical properties of hydrogels

[0082] The compositions according to Examples 1 to 5 and Comparative Examples 1 to 15 were evaluated for the physical properties of the hydrogels, such as swelling property, wettability, and strength. The results are shown in Table 4.

[0083] The experimental method was as follows. For the swelling property, samples of the Example and Comparative Examples were immersed in water at the same weight, and the change in weight was measured to evaluate the swelling property. The larger the weight after immersion in water, the more water the hydrogel absorbed, and the better the swelling property.

[0084] The wettability of the samples of the Examples and Comparative Examples was evaluated by measuring the degree of water evaporation after exposing them to the outside for 24 hours. The lower the absolute value of the measured (-) value, the less water evaporated from the hydrogel, and the better the wettability.

[0085] The strength of the hydrogel was measured using a RHEOTECH FUDOH RHEO METER RTC-3005 D rheometer. If the strength is too high and exceeds a certain value, the hydrogel breaks easily. Therefore, if the strength is 20 or less, the hydrogel is considered to be hard and has excellent properties.

[0086] [Table 4]

[0087] As a result of the experiment, in the cases of Examples 1 to 5, the swelling was measured at a value of 20 or more, the absolute value of the wettability was measured at a value of 30 or less, and the strength was measured at a value of 20 or less, indicating that the swelling, wettability, and strength of the hydrogels were very excellent.

[0088] On the other hand, in the case of Comparative Examples 1 to 11, in which one or more of the components constituting the first gelling agent and the second gelling agent were omitted, the swelling was measured at a value less than 20, the absolute value of the wettability was measured at a value greater than 30, or the strength was measured at a value greater than 20, confirming that at least one of the physical properties of the hydrogel among swelling, wettability, and strength was poor.

[0089] In addition, in the cases of Comparative Examples 12 to 15, in which the first gelling agent and the second gelling agent were included in weight ratios outside the ranges of the Examples, the swelling was measured at a value less than 20, the absolute value of the wettability was measured at a value greater than 30, or the strength was measured at a value greater than 20, confirming that at least one of the physical properties of the hydrogel among swelling, wettability, and strength was poor.

[0090] (2) Experimental Example 2: Evaluation of Usability

[0091] In order to evaluate the feel of use of the compositions according to Examples 1 to 5 and Comparative Examples 1 to 15, 30 evaluators in their 20s to 40s evaluated the rolling feel and residual feel, and the results are shown in Table 5.

[0092] The experimental method is as follows: After applying the samples of the Examples and Comparative Examples to the skin of the evaluators, the evaluation items were evaluated on a scale of 5 levels, and scores were given in increments of 1 point for each level out of a maximum of 5. The usability was evaluated by calculating the average value of the total scores and excluding the deviation.

[0093] [Table 5]

[0094] As a result of the experiment, it was found that Examples 1 to 5 all received high marks in the rolling sensation and residual sensation categories, providing an excellent sensation when used.

[0095] On the other hand, in the case of Comparative Examples 1 to 11, in which one or more of the components constituting the first gelling agent and the second gelling agent were omitted, the scores for the rolling sensation and residual sensation items were all low, confirming that the sensation of use was poor.

[0096] In addition, in the cases of Comparative Examples 12 to 15, in which the first gelling agent and the second gelling agent were included in amounts outside the weight ratio range of the Examples, the scores for the rolling sensation and residual sensation items were all low, confirming that the sensation of use was poor.

[0097] Although specific elements of the present invention have been described in detail above, it will be apparent to those skilled in the art that such specific descriptions are merely preferred embodiments and are not intended to limit the scope of the present invention. Therefore, the true scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. 1. A hydrogel composition comprising: a first gelling agent; and a second gelling agent; The first gelling agent includes alginate, carrageenan, and gellan gum, The second gelling agent is succinoglycan. A hydrogel composition comprising:

2. The alginate, carrageenan, gellan gum, and succinoglycan are contained in a weight ratio of 1:0.5-8:0.5-2:0.5-4. The hydrogel composition of claim 1 .

3. The alginate is contained in an amount of 0.01 to 10% by weight based on the total weight of the hydrogel composition. The hydrogel composition of claim 1 .

4. The carrageenan is contained in an amount of 0.01 to 10% by weight based on the total weight of the hydrogel composition. The hydrogel composition of claim 1 .

5. The gellan gum is contained in an amount of 0.01 to 10% by weight based on the total weight of the hydrogel composition. The hydrogel composition of claim 1 .

6. The succinoglycan is contained in an amount of 0.01 to 10% by weight based on the total weight of the hydrogel composition. The hydrogel composition of claim 1 .

7. The first gelling agent may be selected from the group consisting of glucomannan, xanthan gum, gelatin, chitosan, collagen, hyaluronic acid, gum arabic, pullulan, pectin, cellulose, sclerotium gum, tara gum, agar, locust bean gum, and guar gum. Tetragonoloba (Guar) Gum) The hydrogel composition of claim 1 .

8. Further containing at least one of a hardener and a moisturizer. The hydrogel composition of claim 1 .

9. The curing agent contains at least one of a divalent metal ion, a trivalent metal ion, and a metal salt. The hydrogel composition of claim 8.

10. The metal ions are calcium (Ca ２＋ ), magnesium (Mg ２＋ ), barium (Ba ２＋ ), manganese (Mn ２＋ ), nickel (Ni ２＋ ), copper (Cu ２＋ ), zinc (Zn ２＋ ), iron (Fe ３＋ ) and aluminum (Al ３＋ ) and one or more selected from the group consisting of The hydrogel composition of claim 9.

11. The metal salt includes at least one selected from the group consisting of calcium chloride, calcium hydroxide, calcium nitrate, and calcium sulfide. The hydrogel composition of claim 9.

12. The curing agent is contained in an amount of 0.01 to 5% by weight based on the total weight of the hydrogel composition. The hydrogel composition of claim 8.

13. The moisturizer includes at least one selected from the group consisting of glycerin, propylene glycol, dipropylene glycol, butylene glycol, pentylene glycol, and sorbitol. The hydrogel composition of claim 8.

14. The humectant is contained in an amount of 0.01 to 35% by weight based on the total weight of the hydrogel composition. The hydrogel composition of claim 8.

15. 1. A method for producing a hydrogel composition, comprising: Mixing a first gelling agent and a second gelling agent with purified water to obtain a mixture; and dropping the mixture into a curing agent; The first gelling agent includes alginate, carrageenan, and gellan gum, The second gelling agent is succinoglycan. A method characterized by:

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